CN103060362A - Cloning method of antibiotics biosynthetic gene cluster based on site-specific recombination - Google Patents

Abstract

The invention belongs to the field of biotechnology and genetic engineering, and specifically discloses a cloning method of antibiotics biosynthetic gene cluster based on site-specific recombination. The method comprises the following steps of: based on the site-specific recombination reaction mediated by the integrase encoded by the streptomycete bacteriophage Phi BT1, firstly, executing genetic modification on the strains, which hare generated by the antibiotics, by means of homologous recombination mode; respectively inserting the BAC (Bacterial Artificial Chromosome) vector, the resistant gene and the site-specific recombination sites attB and attP identified by the Phi T1 integrase at two ends of the targeted gene cluster, and extracting chromosome genome segments; under the action of the Phi T1 integrase, causing site-specific recombination reaction between the sites attB and attP in vitro; enabling the target gene cluster to cyclize itself, thereby realizing clone of dozens or even hundreds of kb of large-segment antibiotics biosynthetic gene clusters. The cloning method of antibiotics biosynthetic gene cluster based on site-specific recombination lays good foundation for further genetic modification of the biosynthetic gene clusters, and has better application foreground.

Description

A kind of cloning process of the microbiotic biological synthesis gene cluster based on locus specificity restructuring

Technical field

The invention belongs to gene engineering technology field, be specifically related to a kind of method based on φ BT1 intergrase system and homologous recombination technique realization large fragment biological synthesis gene cluster clone.

Background technology

Natural product plays an important role in the discovery of medicine and evolution always.The main medicine of human treatment's disease in the past in decades always.Yet, being widely used along with antibiotic, bacterium has produced resistance gradually, meanwhile becomes more and more difficult with traditional method from occurring in nature screening new type natural product, thereby is difficult to satisfy the demand of disease treatment.Therefore, find new have bioactive natural product and analog thereof, become the study hotspot of biology, chemistry and the world of medicine.Chemosynthesis is as increasing the multifarious traditional method of Structures of Natural Products, and technique is numerous and diverse, and cost is higher.Along with molecular biological development, the theory of Combinatorial biosynthesis is suggested, and becomes just gradually the emphasis of medicament research and development.Combinatorial biosynthesis is by coming the complex compound of biological synthesizing new to the Genetic Control of natural product pathways metabolism: on the one hand, the biosynthetic pathway of genetic modification natural product specifically, obtain the gene recombination bacterial strain with this, produce needed natural product and analog thereof; On the other hand, the natural product biosynthesis gene of different sources is recombinated, set up the novel pathways metabolism of combination in microbe, the new type natural product that produces of recombinant microorganism storehouse has consisted of the analogue storehouse thus, is conducive to find and develop the medicine that has more using value.Compare with chemosynthesis, its target product can be produced by the recombinant bacterial strain fermentation is a large amount of, thereby has reduced production cost and environmental pollution.

The research of microbiotic biosynthetic pathway and biological synthesis gene cluster has had nearly 30 years history, and in the meantime, the microbiotic synthetic gene of many outbalances is bunch all by note.Find that wherein more microbiotic biological synthesis gene cluster has similar structure gene and arrayed feature, particularly PKS, NRPS class microbiotic is all arranged with the order of moudle type.This clone who is characterized as gene cluster lays a good foundation.Yet the microbiotic synthetic gene is bunch larger, can reach tens even hundreds of kb, the large like this gene cluster of clone that the carriers such as Fosmid can not be complete, thereby in the transformation process to microbiotic synthetic gene bunch, only relate to disappearance and the replacement of indivedual genes.In addition, there is considerable microorganism hereditary operation background not known by people, even if the relatively many streptomycetes of research also only have the minority bacterium can carry out easily genetic manipulation.And the rare actinomycete of some important secondary metabolites of numerous generations all lacks effective conversion means, carries out the very loaded down with trivial details and difficult success of genetic modification in former host.

The present invention utilizes the effect of phage phi BT1 intergrase, makes the locus specificity recombination site that is inserted in microbiotic synthetic gene bunch two ends AttBWith AttPThe locus specificity recombining reaction occurs, gene cluster recirculation, and then the clone who finishes target gene bunch.Because in the site AttBWith AttPBetween include BAC carrier and resistance screening mark, can realize very easily the clone operations of large fragment gene cluster.

Summary of the invention

The biological synthesis gene cluster that the objective of the invention is to overcome large fragment is difficult to the problem with the traditional method clone, and the method for a kind of clone's microbiotic synthetic gene based on Streptomyces Phage φ BT1 intergrase system bunch is provided.

The method of the clone's microbiotic synthetic gene that provides bunch at first makes up two homologous recombination vectors of target gene bunch upstream and downstream, the site that φ BT1 intergrase is identified AttBWith AttPBe inserted in respectively the both sides of target gene bunch; By the integrase mediated locus specificity recombining reaction of φ BT1, just can make the gene cluster recirculation, thereby finish the clone of gene cluster again.

The present invention is take the clone of erythromycin gene cluster as example, and concrete steps are as follows:

(1) structure of erythromycin gene cluster upstream homologous recombination vector pEry-up

With red saccharopolyspora Saccharopolyspora erythraeThe genome of a is template, be the erythromycin gene cluster upstream homology arm fragment of 1993bp with primer SEQ ID No. 2 and primer SEQ ID No. 3 amplification sizes, be the T-A clone with pMD19-T carrier (TAKARA company), obtain plasmid T-ery-up, choose the contiguous direction in XbaI site on MunI site and the T carrier.Plasmid pTARBAC2.1[1] and plasmid pDZY101[2] carry out double digestion with NheI, EcoRI and XbaI, EcoRI respectively, producing size is two bar segment of 7478bp and 4178bp, connects the purpose fragment, obtains plasmid pBAC-DZY101.Plasmid pBAC-DZY101 digests with NheI and EcoRI again, and producing size is the purpose fragment of 9500bp; Plasmid T-ery-up digests with MunI and XbaI, and the generation size is 4683 purpose fragment, connects the fragment of two clauses and subclauses, obtains plasmid pEry-up, and sequence is shown in SEQ ID No. 4.

(2) homology of carrier pEry-up is inserted

The carrier pEry-up that builds is converted into intestinal bacteria ET12567, picking positive transformant and red saccharopolyspora Saccharopolyspora erythraeA carries out conjugal transfer, behind 30 ℃ of cultivation 16 ~ 20h, covers the sterilized water that lml contains nalidixic acid (final concentration is 50 mg/ml) and kantlex (final concentration is 30 mg/ml) at planar surface, cultivates picking zygote more than 5 days for 30 ℃.

(3) structure of erythromycin gene cluster downstream homologous recombination vector pEry-down

Take plasmid pSET152 as template, be the fragment of 2283bp with primer SEQ ID No. 5 and primer SEQ No. 6 amplification sizes, be the T-A clone with the pMD19-T carrier and obtain plasmid pT-SET152.Take plasmid pCY104[3] be template, be the fragment of 847bp with primer SEQ ID No. 7 and primer SEQ ID No. 8 amplification sizes, be T-A clone with the pMD19-T carrier, obtain plasmid pT-CY104, select the direction of HindIII site away from the T carrier of NheI site on the PCR product fragment.Respectively plasmid pT-SET152 and plasmid pT-CY104 are carried out double digestion with restriction enzyme HindIII, XbaI and HindIII, NheI again, producing size is the fragment of two clauses and subclauses of 2315bp and 870bp, obtains plasmid pCY104-SET152 after the connection.With red saccharopolyspora Saccharopolyspora erythraeThe genome of a is template, is the erythromycin gene cluster downstream homology arm fragment of 2194bp with primer SEQ ID No. 9 and primer SEQ ID No. 10 amplification sizes, is the T-A clone with the pMD19-T carrier, obtains plasmid pT-ery-down.Plasmid pT-ery-down and pCY104-SET152 further carry out respectively double digestion with restriction enzyme XbaI, MunI and XbaI, EcoRI again, producing size is the fragment of two clauses and subclauses of 2200bp and 3129bp, obtain plasmid pEry-down after the connection, sequence is shown in SEQ ID No. 11.

(4) homology of carrier pEry-down is inserted

The carrier pEry-down that builds is converted into intestinal bacteria ET12567, picking positive transformant and the red saccharopolyspora that has inserted upstream homologous vector pEry-up Saccharopolyspora erythraeA carries out conjugal transfer, behind 30 ℃ of cultivation 16 ~ 20h, covers the sterilized water that lml contains nalidixic acid (final concentration is 50 mg/ml) and apramycin (final concentration is 30 mg/ml) at planar surface, cultivates picking zygote more than 5 days for 30 ℃.

(5) clone of the erythromycin synthetic gene that mediates of the extracting of red saccharopolyspora chromogene group and external φ BT1 intergrase bunch

The red saccharopolyspora genome of homologous vector pEry-up and pEry-down has been inserted in extracting, guarantees that genomic fragment comprises whole erythromycin gene cluster sequences.30 ° of C in specific buffer are incubated overnight with genomic fragment and φ BT1 intergrase.Under the effect of φ BT1 intergrase, erythromycin gene cluster both sides AttBWith AttPThe locus specificity recombining reaction occurs in the site, and then obtains comprising the complete sequence of erythromycin synthetic gene bunch, is designated as plasmid pZB201, its sequence such as SEQ IDNo. shown in 1.

The inventive method is simple and efficient, can be widely used in the clone operations of microbiotic synthetic gene in the streptomycete bunch.In addition, for the important secondary metabolism the production of material bacterium of can producing of other, such as slime bacteria, mould etc., as long as revise accordingly at skeleton carrier, add the required element of corresponding experimental subjects, obtain corresponding homologous recombination vector, can realize the clone operations of corresponding gene bunch, be not subjected to the restriction of host type.

Description of drawings

Fig. 1: the structure iron of plasmid pZB201.

Fig. 2: the structure iron of Erythromycin gene cluster upstream homologous recombination vector pEry-up.

The collection of illustrative plates mark	Corresponding gene or function	The position
			eryK	Cytopigment p450, the erythromycinB/D hydroxylase	668-1861
ori	The replicon of intestinal bacteria pUC19 carrier	3038-2450
			amp	Ammonia benzyl resistant gene	4069-3209
aphII	Kalamycin resistance gene	5817-4893
			oriT	The conjugal transfer element	6274-6385
BattP	The specificity recombination site that φ BT1 intergrase is identified	6594-6602
			BAC	The BAC carrier is with chloramphenicol resistance gene	6720-14183

Fig. 3: the structure iron of Erythromycin gene cluster downstream homologous recombination vector pEry-down

。

Fig. 4: the Erythromycin gene cluster clone process synoptic diagram that φ BT1 integrase mediates

Carrier pEry-up and pEry-down are inserted into the both sides of erythromycin gene cluster by the mode of homologous recombination, be positioned at the BattB of both sides and BattP site at external effect generation locus specificity recombining reaction through φ BT1 integrase, gene cluster recirculation, and then the clone who finishes the erythromycin gene cluster.

Fig. 5: the pulsed field gel electrophoresis result after plasmid pZB201 cuts with restriction enzyme HindIIII enzyme

Lane 1 ~ 7 is the clone of 7 pZB201 of picking, M be λ- EcoT14 I digest Marker.

Embodiment

The clone of the erythromycin biological synthesis gene cluster of the present invention in the red saccharopolyspora is as example, the concrete method of introducing clone's microbiotic synthetic gene bunch, and detailed operating process is as follows:

1. the structure of erythromycin gene cluster upstream homologous recombination vector pEry-up

With red saccharopolyspora Saccharopolyspora erythraeThe genome of a is template, with primer SEQ ID No. 2 and primer SEQ ID No. 3 amplification erythromycin gene cluster upstream homology arm fragments, clip size is 1993bp, SEQ ID No. 2 is designed with MunI restriction enzyme enzyme recognition site, PCR product fragment and pMD19-T carrier (TAKARA company) are done the rear conversion of T-A clone intestinal bacteria, the picking positive transformant also extracts plasmid DNA, choose the plasmid of the direction of the XbaI site vicinity on MunI site and the T carrier, called after T-ery-up.Plasmid pTARBAC2.1 and plasmid pDZY101 carry out double digestion with NheI, EcoRI and XbaI, EcoRI respectively, and producing size is two fragments of 7478bp and 4178bp, carries out agarose gel electrophoresis, reclaim the purpose fragment.Use T ₄Dna ligase (TAKARA company) connects the fragment of two clauses and subclauses after reclaiming, and transforms intestinal bacteria, and the picking positive transformant also extracts plasmid DNA, called after pBAC-DZY101.Plasmid pBAC-DZY101 digests with NheI and EcoRI again, and producing size is the purpose fragment of 9500bp; Plasmid T-ery-up digests with MunI and XbaI, and the generation size is 4683 purpose fragment, carries out agarose gel electrophoresis, reclaims the purpose fragment.Use T ₄Dna ligase connects the fragment that reclaims, and transforms intestinal bacteria, and the picking positive transformant also extracts plasmid DNA, is pEry-up, and sequence is shown in SEQ ID No. 4.

2. the homology of carrier pEry-up is inserted

The carrier pEry-up that builds is converted into intestinal bacteria ET12567, the picking positive transformant.To contain recombinant plasmid E.coliET12567 is inoculated in the 50m1LB liquid nutrient medium and (contains corresponding microbiotic), is cultured to OD600=0.4 ~ 0.6, with bacterium liquid centrifugal 5min of 5000rmp in the 50ml centrifuge tube, outwell supernatant, add the 30mlLB washed twice, then be resuspended among the 2mlLB, as the donor bacterium of conjugal transfer.Get an amount of frozen in-80 ℃ S.erythraeaGlycerine spore suspension 500ml, with isopyknic TES buffer washed twice, be resuspended among the TES buffer of 500ml, make spore germination at 50 ℃ of heat shock 10min, add again the TSB of 500ml, 37 ℃ of incubation 2 ~ 5h.Centrifugally be resuspended in conduct among the 0.5mlLB S.erythraeaRecipient cell.Recipient cell 100 ml of same volume are mixed with the donorcells of different volumes directly to be coated on contain 10mM MgCl ₂The MS flat board on, 30 ℃ cultivate 16 ~ 20h after, cover the sterilized water that lml contains nalidixic acid (final concentration is 50 mg/ml) and kantlex (final concentration is 30 mg/ml) at planar surface, 30 ℃ of cultivations picking zygote more than 5 days.

3. the structure of erythromycin gene cluster downstream homologous recombination vector pEry-down

Take plasmid pSET152 as template, be the fragment of 2283bp with primer SEQ ID No. 5 and primer SEQ No. 6 amplification sizes, SEQ ID No. 5 is designed with that φ BT1 intergrase identifies AttBThe site, SEQ ID No.6 is designed with EcoRI restriction enzyme enzyme recognition site, and PCR product fragment and pMD19-T carrier are done the rear conversion of T-A clone intestinal bacteria, and the picking positive transformant also extracts plasmid DNA, is pT-SET152.Take plasmid pCY104 as template, be the fragment of 847bp with primer SEQ ID No. 7 and primer SEQ ID No. 8 amplification sizes, PCR product fragment and pMD19-T carrier are done the rear conversion of T-A clone intestinal bacteria, the picking positive transformant also extracts plasmid DNA, select NheI site on the PCR product fragment on the T carrier HindIII site than a distant place to plasmid, called after pT-CY104.Respectively plasmid pT-SET152 and plasmid pT-CY104 are carried out double digestion with restriction enzyme HindIII, XbaI and HindIII, NheI again, producing size is two fragments of 2315bp and 870bp, carries out agarose gel electrophoresis, reclaims the purpose fragment.Use T ₄Dna ligase connects the fragment of two clauses and subclauses after reclaiming, and transforms intestinal bacteria, and the picking positive transformant also extracts plasmid DNA, called after pCY104-SET152.With red saccharopolyspora Saccharopolyspora erythraeThe genome of a is template, with primer SEQ ID No. 9 and primer SEQ ID No. 10 amplification erythromycin gene cluster downstream homology arm fragments, clip size is 2194bp, SEQ ID No. 9 and SEQ ID No. 10 are designed with respectively XbaI and MunI restriction enzyme enzyme recognition site, PCR product fragment and pMD19-T carrier are done the rear conversion of T-A clone intestinal bacteria, the picking positive transformant also extracts plasmid DNA, is pT-ery-down.Plasmid pT-ery-down and pCY104-SET152 further carry out respectively double digestion with restriction enzyme XbaI, MunI and XbaI, EcoRI again, and producing size is the fragment of two clauses and subclauses of 2200bp and 3129bp, and electrophoresis is used T after reclaiming ₄Dna ligase connects, and transforms intestinal bacteria, and the picking positive transformant also extracts plasmid DNA, is pEry-down, and sequence is shown in SEQ ID No. 11.

4. the homology of carrier pEry-down is inserted

The carrier pEry-down that builds is converted into intestinal bacteria ET12567, the picking positive transformant.According to the described method of step 2, to contain recombinant plasmid pEry-down's E.coliET12567 is the donor bacterium, to have inserted upstream homologous vector pEry-up's S.erythraeaSpore suspension is that recipient bacterium carries out conjugal transfer, behind 30 ℃ of cultivation 16 ~ 20h, cover the sterilized water that lml contains nalidixic acid (final concentration is 50 mg/ml) and apramycin (final concentration is 30 mg/ml) at planar surface, cultivate picking zygote more than 5 days for 30 ℃.

5. the extracting of red saccharopolyspora chromogene group

The spore suspension 100 μ l that inserted the red saccharopolyspora of homologous vector pEry-up and pEry-down are inoculated in the 3mlYEME liquid nutrient medium cultivate 48h, the centrifugal collection thalline of 12000rpm, sterilized water Eddy diffusion thalline with 200 μ l, adding final concentration is the N,O-Diacetylmuramidase of 3mg/ml, 37 ℃ of water bath heat preservation 30 min.The SDS solution that adds 400 μ l 10%, adding final concentration behind the jog mixing is 20mg/ml Proteinase K solution again, behind the mixing in 55 ° of C water bath heat preservation 30 min.Isopyknic phenol chloroform extracting 2 ~ 3 times, draw the upper strata water in new 1.5mlEP pipe, isopropanol precipitating 30 min that add 0.6 times of volume, centrifugal 10 min of 12000rpm outwell supernatant, and precipitation is with twice of 70% washing with alcohol, centrifugal 2 min of 12000rpm, outwell supernatant, after the ethanol volatilization is done, with the sterilized water dissolving of 30 μ l.

6. the clone of the erythromycin synthetic gene that mediates of external φ BT1 intergrase bunch

Vitro recombination reaction: the genomic fragment, the 1 μ l 0.1M Tris buffer(PH 8.0 that comprise 3 μ l in the 10 μ l reaction systems), 2 μ l 0.5M KCl solution and 0.5 μ l φ BT1 integrase, with ddH ₂O supplies cumulative volume to 10 μ l.For guaranteeing reaction efficiency, 30 ° of C are incubated overnight; For removing macromolecular synaptonemal complex, reaction is processed 30 min with proteinase K in 55 ° of C later, again in 80 ° of C deactivation proteinase K 20 min, obtain cloning the complete sequence that comprises erythromycin synthetic gene bunch, be designated as plasmid pZB201, its sequence is shown in SEQID No. 1.

The recombinant products of reaction is converted in the intestinal bacteria DH10B bacterial strain, coats the solid LB substratum that contains apramycin, 37 ° of C overnight incubation.Select seven and cut checking with restriction enzyme HindIII enzyme in the clone who grows out, it is all correct that enzyme is cut the result.The order-checking of sample of picking is with the exactness of further checking positive colony, and sequencing result has verified that institute's DCRP comprises the complete sequence of erythromycin synthetic gene bunch.

Reference:

1. Osoegawa K, Zhu B, Shu CL, Ren T, Cao Q, et al. (2004) BAC resources for the rat genome project. Genome Res 14: 780-785.

2. Zhang X, Bao Y, Shi X, Ou X, Zhou P, et al. (2012) Efficient transposition of IS204-derived plasmids in Streptomyces coelicolor. J Microbiol Methods 88: 67-72.

3. Wensheng Yao YY, Juishen Chiao (1994) Cloning Vector System for Nocardia spp. Current Microbiology. 29:223-227。

＜110〉Fudan University

＜120〉a kind of cloning process of the microbiotic biological synthesis gene cluster based on locus specificity restructuring

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catcagattt tctgatctgg tcaacgaaca gatacagcat acgtttttga tcccgggaga 5040

gactatatgc cgcctcagtg aggtcgtttg actggacgat tcgcgggcta tttttacgtt 5100

tcttgtgatt gataaccgct gtttccgcca tgacagatcc atgtgaagtg tgacaagttt 5160

ttagattgtc acactaaata aaaaagagtc aataagcagg gataactttg tgaaaaaaca 5220

gcttcttctg agggcaattt gtcacagggt taagggcaat ttgtcacaga caggactgtc 5280

atttgagggt gatttgtcac actgaaaggg caatttgtca caacaccttc tctagaacca 5340

gcatggataa aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat 5400

aattataaaa atatccccgt ggataagtgg ataaccccaa gggaagtttt ttcaggcatc 5460

gtgtgtaagc agaatatata agtgctgttc cctggtgctt cctcgctcac tcgagggctt 5520

cgccctgtcg ctcaactgcg gcgagcacta ctggctgtaa aaggacagac cacatcatgg 5580

ttctgtgttc attaggttgt tctgtccatt gctgacataa tccgctccac ttcaacgtaa 5640

caccgcacga agatttctat tgttcctgaa ggcatattca aatcgttttc gttaccgctt 5700

gcaggcatca tgacagaaca ctacttccta taaacgctac acaggctcct gagattaata 5760

atgcggatct ctacgataat gggagatttt cccgactgtt tcgttcgctt ctcagtggat 5820

aacagccagc ttctctgttt aacagacaaa aacagcatat ccactcagtt ccacatttcc 5880

atataaaggc caaggcattt attctcagga taattgtttc agcatcgcaa ccgcatcaga 5940

ctccggcatc gcaaactgca cccggtgccg ggcagccaca tccagcgcaa aaaccttcgt 6000

gtagacttcc gttgaactga tggacttatg tcccatcagg ctttgcagaa ctttcagcgg 6060

tataccggca tacagcatgt gcatcgcata ggaatggcgg aacgtatgtg gtgtgaccgg 6120

aacagagaac gtcacaccgt cagcagcagc ggcggcaacc gcctccccaa tccaggtcct 6180

gaccgttctg tccgtcactt cccagatccg cgctttctct gtccttcctg tgcgacggtt 6240

acgccgctcc atgagcttat cgcgaataaa tacctgtgac ggaagatcac ttcgcagaat 6300

aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat 6360

gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac 6420

cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa 6480

aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 6540

catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 6600

ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 6660

cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt gagctggtga 6720

tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 6780

cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 6840

tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 6900

tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 6960

acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 7020

tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa 7080

tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag 7140

gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga 7200

tgaatggcag aaattcgatg ataagctgtc aaacatgaga attggtcgac ggcgcgccaa 7260

agcttgcatg cctgcagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg 7320

atgccctgcg taagcggggc acatttcatt acctctttct ccgcacccga catagataat 7380

aacttcgtat agtatacatt atacgaagtt atctagtaga cttaattaag gatcgatccg 7440

gcgcgccaat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca 7500

agggcatcgg tcgagcttga cattgtagga ctatattgct ctaataaatt tgcggccgct 7560

aatacgactc actataggga gaggatcttc atagggaatt gctcacgcaa gagccaagct 7620

gagttcgtct gcgtgtcctg cggtttcacc tgcaacgctg acctgaacgc ctcgatcaac 7680

gtcgcggcag gacatgccgg agggacaaca acgacccctg gtcgttcacg tgtccgtgaa 7740

cctcaactcg ccctccggtg agttggaatc ccccgcctcc aggcgaggga ggatgtcaaa 7800

tccagaagac accggtggtc ttcgacgggg agctgacgct gctgcccatg gggcagtacc 7860

tgcaccgcgc cctcggcggc gactacgtcg ccctcgccgc cacgcacacc ggcaccagcg 7920

cccccgagat agagctcgac gacagcagcg acagcggctt cgccgttcgg gaggtggatc 7980

tgcccgcacc ggaggacgga agcatcgagg ccgcggccgt cgcggcgcgc atcggaacgg 8040

gcctcgtcga cctgcgcgcg caagctcccg gcctggaccg gatccgctcc cagagcacgt 8100

ggatgcggac gccgctgcgc gacgcgttcg acgccgtgct cactgttccc accgccaccg 8160

cggaagtctc gacaccccgt ccggcgcgcg actaagctca agatcgcgac gcgtgttcgc 8220

cgacgtggaa acgacctgct gcgcgaggag aacgttgacc accatcgacg aagttcccgg 8280

catggccgac gaaaccgcac tcctcgactg gctgggcacg atgcgcgaga agcagccggt 8340

gtggcaggac cggtacggcg tgtggcacgt cttccgccac gccgacgtcc agacggtgtt 8400

gcgcgacacc gccacgttct cctccgaccc gacccgcgtc atcgagggcg cgagcccgac 8460

gccgggcatg atccacgaga tcgacccgcc ggagcaccgc gccctgcgca aggtcgtcag 8520

cagcgccttc accccgcgca ccatctccga cctcgaaccc cgcatccgcg acgtcacccg 8580

ctcgctgctg gccgatgccg gcgagagctt cgacctcgtc gacgtgctcg cgttcccgct 8640

gccggtcacg atcgtcgcgg agctgctggg cctgccgccg atggaccacg agcagttcgg 8700

cgactggtcg ggtgccctgg tcgacatcca gatggacgac ccgaccgacc cggcgctggc 8760

cgaacggatc gcggatgtgc tcaacccgct caccgcgtac ctgaaggccc gctgcgccga 8820

gcgccgcgcc gatcccggcg acgacctgat ctcccgcctt gtcctggccg aggtcgacgg 8880

gcgcgcgctc gacgacgagg aggcggccaa cttctccacc gcgctgctgc tggccggcca 8940

catcaccacg accgtcctgc tcggcaacat cgtgcgcacc ctcgacgagc acccggccca 9000

ctgggacgcc gccgccgagg accccggccg gatcccggcg atcgtcgagg aggtcctgcg 9060

ctaccggccg ccgttcccgc agatgcagcg cacgacgacc aaggccaccg aggtcgcggg 9120

cgtgcccatc ccggccgacg tcatggtcaa cacctgggtg ctctcggcca accgcgactc 9180

cgacgcccac gacgaccccg accggttcga cccgtcgcgc aagtccggcg gcgccgcgca 9240

gctctccttc ggccacggcg tgcacttctg cctcggcgcg cccctggccc ggctggagaa 9300

ccgcgtggcg ctggaggaga tcatcgcccg gttcgggcgg ctcaccgtcg accgcgacga 9360

cgagcgcctg cgccacttcg aacagatcgt gctcgggacc cgtcacctgc cggtgctggc 9420

gggctcctcg ccgaggcagt cggcgtagtc gggcagcatc cctcgccgcc tggcggtctc 9480

caggtccacg gccaccgtgt cgcggtcgga gagcacgacc tccaggtccg cgggccacgg 9540

cagacccagc gcggggtcga acgggtcgat cgcctgctcg tactcggcca cgtacggcgt 9600

gctgaccagg tacgacatca gggtgtcgtc ctcaagcgcg aggaaggcgt gcgcggtgcc 9660

cctggggaag tagaccgccc ggaagtgctc ggtgtccatc tccaccgcgt cccacttccc 9720

gaacgtcggc gagccgaccc ggatgtcgac gatgacgtcg agcgcccggc cgcgcgcgca 9780

gtagacgtac ttgcactgcc ccggcggggt ggtggtgaag tgcagcccgc gcagcacgcc 9840

ccgggcggag acgatgtggt tcatctgggc gacggggaac cgatggccca ccgcgcccac 9900

gaacgcctcg tcctgcagcg gagacacgaa caggccccgc cggtcctgct tcgggtcggg 9960

ggtgaactcg aaaccgcccg ccaccgccag ctcacgtact cgcaactccg gttcaccttc 10020

cgcgcaggaa gccgcggtgg tcctcccacc cgtcggcgat gtctcgttcg agctggttga 10080

gccgtgcggt caccgactgg tcgaagccgt cgaggaagta ctcgtcgccg gggcggggct 10140

gcacgccctc gttctccagc acgaagttct ccaccacctc gcgcaggctg gtcgacgggg 10200

tgacccggcc cgcgatgtag cgggtggcgc cctccagctc cgggaagccc gcctcgcggt 10260

agaggtagac gtcgccgagc aggtcgatct gcaccgcgac ctgggggtgc gcggtgggac 10320

gcatcgtctc cggccggatg cgcagcagct cggcgtccac accccgccgc aggctctcca 10380

gggcgtagcc caggtcgatg tgcatgcccg gggtccgctc ggcggcgtag tcgacgaacc 10440

gcaccaggcc ctcgcgcagc tcgttgcgct cggagtccga cagccggccg tcgtcgcggc 10500

cgctgtagtc ctcgcgcacc gtcacgaagt ccagcggccg ttgcgggctg gactcgttga 10560

gctcggcgat gaagtcgacg aggtcggtga gccggtcggc ccgtcccggc aggatgatgt 10620

ggttgaagcc gagccggatc ggcgcgtccc gctcggcgcg catccgcagg aagccctgca 10680

ggttcttctt gacgcgttcg aaagcgccgc gcttgccggt ggtcgtctcg tactcgtcgt 10740

tgttcagccc gtagagggac gtgcggatcg cgcccagctc ccacaggccg ggctggcggt 10800

tcagcgtctg ctcggtgagg gcgaaggcgt tggtgtagac ggtgaggtcg aaaccgcgcc 10860

cggcggcgtg cgacaccagc tcgccgagac cggggttggt cagcggctcg agcccgcccg 10920

acatgtacat cgccttcggg ttgtccgtgg gcacctcgtc gatgatcgcg gccagcgtct 10980

cgttgcccgc cgggaccgat gcggcctcgt agcgggcacc ggtcacccgc acgcagaagt 11040

ggcagcggaa catgcacgtc ggcccggggt acaggccgac gctgtagggg aaggcaggct 11100

tgcggtagac cgccgcgtcc agtgcgcctg cggcgtccag cggcttgatc gtgttggacc 11160

agtacttgcc cgccggaccg tgctccaccg cggtgcccag gtccgggatg cggccgaaca 11220

ggtcgagcag gtgggcgaac tccgcgcggt ccaggccgag ccgcgcacgc gtctgctcca 11280

gcggcgtgaa cgggtgcgcc ccgtagtgcg cggcgaggtc ggcgagccgg gcggcctgca 11340

cgtcggcgtc accgtcaccg gcgccggtga cgcgggagac ctccgagctc agcgcccgca 11400

cgaccgcccg ccggtcgacg tccgggcgcg ccgcgtcggc gacccccgct tcctgggcgg 11460

ttgccgtacg agttgtgttc atcggggagc gaacctcctg gcacttgtgg cgcaggtcgt 11520

cacgaaccgt tgcgcgtcct ctggtcccat tgcgcgttga tcagctcgcc gctggtggcg 11580

gcgagccgga tgatgtcgca cacccgccgg atgtcctcgc tggacaccgc ggggccggtg 11640

ggcagcgcga gcacccggtc ggagagctgt tcggtgttct ccagccgcag cggcggctcg 11700

gtccggtacg gctgcatctg gtggcacccg ggggagaagt agggttgtgc cacaaccttc 11760

tccgctcgca ggatcgcctg caactggtcg cggtcgatgc cggtggccgc ggagtccacc 11820

gagatgatca cgtactggta gttgttctgc tcgccaggat cgaacgcgtg cacggatatg 11880

ccgcgcacgt cgcggagctc gtcggagtag agcgcgtggt tgaggcggtt gtgcaccctg 11940

gtctcggcga aggcgtcgag cgaggtgagg cccatcgccg cggcgcactc gctcatcttg 12000

ccgttggtgc cgacatcggt caccagcttg tccggtgcga tcccgaagtt gtgcatggcg 12060

cggatgcggt cggccagcag cccgtcgtcg gtgacgatgg cgccgccctc gaacgaggtg 12120

accgccttcg tggcgtggaa gctgaacacc tcggcgttgc cgaaggcgcc gaccggccgc 12180

ccgccggcgg tgcagcccag cgcgtgcgcg gcgtcgaaga agagtttgac ctggtgctcg 12240

gcggcgatct tctccagcgc ctcgaccgga gcgggcctgc cccacaggtg cacgccgatg 12300

atcgcgcccg tccgcggtgt caccagcgac gcgacgtgct cggggtcgag caggccggtc 12360

tcggggtcca cgtcgcagaa caccggttcc agccccagcc agctcgccgc gtgcgcggtg 12420

gccgcgaacg tcatcgaagg catgacgacc tcgccggaca cgtcgctcgc gcgcagcacc 12480

agttgcagcg cgaccgtcgc gttgcaggtg gccacgcagt ggcggacacc cgccaggtcg 12540

gcgacccggc cctcgaactc gcgcaccagt ggtccgccgt tggtcagcca gttgttgttc 12600

agcgcccact ccaggcgggc gaagaaccgc tcccggtccc cgacggtcgg cctgcccacg 12660

tagagggtgt gcaggaatgc ctcggggccg ccgaagatcg ccaggtcggt cagcgcgcgt 12720

ttcatccggc ggtcctggtg tagacggccg aggcgcacgc gatgatgctg cgcagctgga 12780

tgttgacgta gtggctgtgc ccgagcaggt aggtgatctg gtcgaaggtc atccaccggt 12840

ggtcgggagc ggcactggcg tcgaagtcgg cgggcacctc gatcagcatg taccggttct 12900

cgttgcggta gaaccggccg ccctcctcgg agtgccatgc gtcgtagcgg acctgcgagc 12960

gcggcacgtt caacacgtag tccacatagg ccggtcggaa ctcctcgggc gcgtcggcgt 13020

agttgtcggg ctggcagtgc accgtaggcg ccagctcggc gacgtccatc ccgcccgcct 13080

cggtgcgcag ctgcacgagc gcgtgcaacg tgccgccgat gtccttgacc agcagggcga 13140

gcaggccgtt gttggcgggc gagagcagcg gctgcatcca cgagttgacc tcgcggtcgc 13200

tggtcgccac cgtgacgccg aagacgtcga agtacttctt ctcctcgtgc tcgatgccgt 13260

cgtcgcggcg gatccacccg ctgcgctcga tgtcgggcag cgggcggccg cgctgcacga 13320

actcgcgcct ggcgcgcacg tcggagatcc agctcagcag gctgttcatg ttgttggccg 13380

gggtgcccgc gccgatgaac gagtgcagca gccgggcctg gaacgagccc tcgggcaggc 13440

gcgcgagcac gtcgtcggcc cggtcccgct ccacggcggt cgggacgcag gccagcacgg 13500

tgcgcaggtc catgttcacc acgttgtcgt ggtgcagcat cgcccgcagc tgcgcgacgg 13560

tcagccaccg gaagttcggg tgctcgggca ggtcgtcgaa cacctcgacg accatgttcc 13620

ggttgcgctt gcgcaggaac cacgcgccct gctcggactg gagcacgtcg acgaggatcc 13680

ggctcgggcg cgtgccgttg aagtactcga tgaaccggac cttcgagccg cggtggacgc 13740

cggtgtagtt gctgcgggtc gcctgcaggg tcggggagag ctggacggcg ttgatgttgc 13800

ccggctcggc cttggcctgc gccagcacgt gcagcacacc gtcgaactcc ttgacgatga 13860

ggccgaggaa gccgatctcg ggctgcacga tgatgggctg gatccagtcc cgccgccagc 13920

cgaagttcgt ccgcacgtgc aggccctcga tggagaagaa gcgcccggac tcgtgggcga 13980

gcctgccgtc ctcgggctgg aacgaccagc ggtccatggc gctgaacggc acgcgcgcca 14040

cctcgagccg ctgctcggcg ccgttgcggg tcagccagtc gtgggtttcc gccgtggcgg 14100

tggggattcc gccgtcgccg agcaggaaac gcgttgcggt ctgcgaggat tccggaatcg 14160

tccggtcgcc ggtccgatca cccatcgact ctccctgcgg tgtcgtcgac ggctccgctt 14220

gctgccgccg ggcgttgtcg atcaagaccc gcactcactg taagggtccg gtcacggcgg 14280

ggaaagggcc cgtggagcgg atcacctgtg ccaggaacgg aaaacgcgaa cgcacgggtc 14340

atccgcgcac accgacgaac aacccgcgtc cggagggtcc gccctccagg tactgcacag 14400

cgcaaccggc cgcggtgaag gccttctcgt actgctgccg ctcgaagagc gtgatctcgt 14460

agtgctccac gtggtgccgc ggaccgttca ccgcgtcggc cacgacccag tggatctcca 14520

tccgggtcgc gccgccggcg cgcacggagt gcgagacgcg cgagatcgtc aggtcgccgt 14580

cgcgcaccac gtcaccggcc acgtagccgt cgaggaagtc ctccgggaac caccacggtt 14640

cgaccaccac gacgccgccg ggggcgaggt ggcgggcgaa ggacgccagc gcctggtcca 14700

gctcggcgcc gtcgcgcatg tgcccgatgg agctgaacat gcaggtgacg gcgtcgaact 14760

cgcgatccag cgcgaagtcg cgcatgtcgc cctgcagcac cgggatgccg ccgagctgcg 14820

gccgggcgac ctcgatcatc gccgccgaca gctccagccc ggtcacgtcg tcgaagaggt 14880

cggcgaaccg gcgcaggtgg gtgccggtcc cgcaggccac gtcgagcagc gaggaagccg 14940

agggcaggcg gtctctgacc agccgcgcga cctgcgcggc ctcggccgcg tagtccttgc 15000

cccggccgcg gtagaaccgg tcgtaaagct cggcgaaccc gccctcgtac atccctgctc 15060

cctccggagg tgtgctgtcg gacggacttg tcggtcggaa accgctagcc ggcgtggcgg 15120

cgcgtgagtt cctccagtcg cgggacgatc tcctgcggcg tcgggtcgga cagcgcctcc 15180

tcgcgaagct tcaccgcgtt ctcggtgtag gtggggtcct cgaccacctg ggtgagggcg 15240

ctcgccaatg agtcggcatc gacctcgtcc ggccggaggt agattcccgc gcccagttcc 15300

gcggtctgct ggccgcgtag catgcaatcc cattcatgtg caactgatat ttgcggaatt 15360

ccgtggtgca gtgccgtggc ccaggttccg gccccgccgt ggtggatgat cgccgcgcag 15420

ttctggagca gaacgcccat cggaacgaaa tccaccaaac gaatgttgtc cggtaccgcg 15480

gaggtatccg gaccggaacc cgtaaccacg atttcgccat cgaatcgcgc gagctgcgcg 15540

agcgtccgcg cgaactgatc ggcatcggcg gcgagcccga gtccggagaa ccctccggta 15600

atgcagatgc gtcgagtcgc actgcccttc ttgagccagt ccggaaccac cgacgcgccg 15660

ttgtagggca gggtccgcgc gacaacggtt tccattccgg tgtccagccg gaaactcggc 15720

ggcaactggt cgaccgacca ctgcccgacc gcgaggtcct cgccgaattc gacgccgaag 15780

cgccccgcga cctcggtcag ccacgtgccc agcgggtccg gccggtcctc cggcggccgt 15840

cgcaggcgtt gcgcctggaa ccggccgcgg aagtagccgg tgaggtcgct gccccacagc 15900

agccgggcgt gcgcggctcc gcaggcccgg gccgcgacgg cgccggcgaa ggtgaacggc 15960

tcccagagca ccaggtcagg acgccagtcc cgggcgaagt cgaccagctc ggcgacgaag 16020

gagtcgttgt tgaccaccgg gtatacccac cgcgaggtgg cctcctgcat gccgagcagg 16080

aactcccacg agtgcagctc ctgctcgcgg tggtagaagt ccaggtagaa ggagtagcgg 16140

tgcacctgag cggcgacttc cgggacgatg tcgaacagcc ggtggtcgga gccgacgggt 16200

accgcggtga gaccggcgcc gatgaccgcg tcggtgagcg cgggctgggc ggccacgcgc 16260

acgtcgtgac ccgcggtgcg cagcgcccac gccagcggga ccagtccctg gaagtgcgtg 16320

cggtgcgcga aggacgtcag cagtacccgc actagtgctc ctcggtgggg gtcagggcgg 16380

ccaccgtccg gtcgatgccg tcggtgaggg aaacccgggg gcgccagccg gtccggctgc 16440

ggaactcggt ggagtcgatg tcgtcgctgc ggaagtcgtt ggcctcggcg tgctcgggcg 16500

cgggcacggt gaccacgtcg acggcggggc tgccggtctg ccgggcgacg ctgccggaga 16560

cggcccggaa gatgtcgccg agcggctcgg atcggtcggc gcccagcgcc cacgtgccgc 16620

cggccagcgc gtcgtggtgc tccagcgcgg cggcgaacgc ggtggccacg tcctcgacgt 16680

gcagcaggtc gcggcgcacg ccgccgtcgt gccacatggt gagcggctcg ccggcgaggg 16740

cacgccggat catcgctgcg accacgcccc gccccatggg gccggacggg ccgctctggc 16800

cgtagacggc gggcagccgc aggatcacgc cgcgcacccg gccctcgtcg gtggctttgc 16860

gcaggatgcg ctcggcctcg gtcttctgct gcgcgtacct gctggccgcc gacgggttcg 16920

cggcctgtgc ggtgctcgcg tagagcaaca cgggcggcgt cgacctgcgg cgatcgtgca 16980

gcgcgccgac gaggtcgtgc atcaggccga cgttgacccg ctcggcttcc gggtcggagg 17040

tggcgctgcg ccaggtggaa ccgcccgctg cgtgcgccac caggtgcacg atcacgtcgg 17100

cgtcctcgat cgcggcggcg gcccggcccg gttccagcag gtcggcgcgc aggtcctcga 17160

cctccgcggc gccgggcgga accgcgggcg ctccgccgcg ggacaccgcg cgcagccgga 17220

ccgggtggtc gcgcagctcg cgcagaaccg cgctcccgac gaagccggaa gcgcccagaa 17280

gggtgatcaa ttgacgcggg gaatcactga tcccattcac cggagcattt gctcgctttc 17340

caggtcggtg ctacgggcga aattcaaaga atctccccag cgcgatgtgc ggcaacccgt 17400

cactgggcca ccacagtagg tagccgccgt tgatcttgtc aacatgcaga tgttcacagg 17460

ttcgttggct cgacgaggcg atgtcaacct cttgatcctt cctatattgt tcgcccattg 17520

cgtggtcgtc gagtaggggg acgcgtggcg gacctgtcaa agctctccga cagtcggact 17580

gcacaacctg ggaggatcgt tcgtccgtgg cccctgtcgg ggtgcaatga atccgccttg 17640

cgggcccgtg cgcgccaatt gcgtgcacat ctcgatcgat ttcccgatgc cggtgtcgaa 17700

ggtgtcgggg ccgcgctcgc gcacgacgag caggcggacg ccggtccgca tcgcgcggtc 17760

gtcgtcgcct cctcgacctc cgagctgctc gacggcctgg ccgccgtcgc cgacggccgg 17820

ccgcacgcct cggtggtccg cggcgtggcc cggccgtccg cgccggtggt gttcgtcttc 17880

ccgggccagg gcgcgcaatg ggccgggatg gcgggcgaac tcctcggcga gtcaagggtt 17940

ttcgccgccg cgatggacgc gtgcgcgcgg gcgttcgagc ccgtgaccga ctggacgctg 18000

gcgcaggtcc tggactctcc cgagcagtcg cgccgcgtcg aggtcgtcca gcccgccctg 18060

ttcgcggtgc agacgtcgct ggccgcgctc tggcgctcct tcggcgtgac ccccgacgcc 18120

gtggtgggcc acagcatcgg cgagctggcc gccgcgcacg tgtgcggtgc ggccggtgcc 18180

gccgacgccg cgcgcgccgc cgcgctgtgg agccgcgaga tgattccgtt ggtgggcaac 18240

ggcgacatgg cagccgtcgc gctctccgcc gacgagatcg agccgcgcat cgcccggtgg 18300

gacgacgacg tggtgctggc cggggtcaac ggtccgcgct cggttctgct gaccgggtcg 18360

ccggaaccgg tcgcgcgccg ggtccaggag ctctcggccg agggggtccg cgcacaggtc 18420

atcaatgtgt cgatggcggc gcactcggcg caggtcgacg acatcgccga ggggatgcgc 18480

tcggccctgg cgtggttcgc gcccggtggc tcggaggtgc ccttctacgc cagcctcacc 18540

ggaggtgcgg tcgacacgcg ggagctggtg gccgactact ggcgccgcag cttccggctg 18600

ccggtgcgct tcgacgaggc gatccggtcc gccctggagg tcggtcccgg cacgttcgtc 18660

gaagcgagcc cgcacccggt gctggccgcc gcgctccagc agacgctcga cgccgagggc 18720

tcctcggccg cggtggtccc gacgctgcaa cgcgggcagg gcggcatgcg gcggttcctg 18780

ctggccgcgg cccaggcgtt caccggcggc gtggccgtcg actggaccgc cgcctacgac 18840

gacgtggggg ccgaacccgg ctctctgccg gagttcgcgc cggccgagga ggaagacgag 18900

ccggccgagt ccggcgtcga ctggaacgcg ccaccgcacg tgctgcgcga gcggctgctc 18960

gcggtcgtca acggcgagac cgccgcgttg gcgggccgcg aagccgacgc cgaggccacg 19020

ttccgcgagc tggggctgga ctcggtgctg gccgcgcagc tgcgcgccaa ggtgagcgcc 19080

gcgatcgggc gcgaggtcaa catcgccctg ctctacgacc acccgactcc gcgtgcgctc 19140

gcggaagcac tcgcggcggg aaccgaggtc gcacaacggg aaacccgcgc gcggaccaac 19200

gaagcggcgc ccggcgaacc ggtcgcggtc gtcgcgatgg cctgccggct gcccggcggt 19260

gtgagcaccc cggaggagtt ctgggagctg ctgtcggagg gccgcgacgc ggtcgcggga 19320

ctgccgaccg accgcggctg ggacctggac tcgctgttcc accccgaccc cacgcgctcg 19380

ggcaccgcgc accagcgcgg cggcggtttc ctgaccgagg cgaccgcgtt cgacccggcc 19440

ttcttcggca tgtccccgcg cgaggcgctg gccgtcgacc cgcagcagcg gctcatgctc 19500

gagctctcct gggaagtgct ggaacgggcg ggaatcccgc cgacctcgtt gcaggcctcg 19560

cccactggcg tgttcgtcgg cctgatcccg caggagtacg gcccgcggct ggccgagggc 19620

ggcgaaggcg tcgagggcta cctgatgacc ggtacgacca cgagcgtcgc ctccggccgc 19680

atcgcctaca cgctcggcct ggagggcccg gcgatcagcg tggacaccgc gtgctcgtcc 19740

tcgctggtcg cggtgcacct ggcgtgccag tcgctgcggc gcggcgagtc gtcgctggcg 19800

atggcaggcg gtgtcacggt gatgccgacg cccggcatgc tggtggactt cagccggatg 19860

aactcgctgg cgccggacgg ccggtgcaag gctttctccg ccggcgccaa cggtttcggc 19920

atggccgagg gcgccgggat gctcctgctg gagcggcttt cggacgcccg ccgcaacggc 19980

cacccggtgc tcgccgtgct cagggggacg gcggtcaact ccgacggcgc gagcaacggg 20040

ctgtcggcgc ccaacgggcg ggcgcaggtg cgggtcatcc agcaggcgct ggcagagtcc 20100

ggtctcgggc ccgccgacat cgacgccgtc gaggcgcacg gcaccggtac ccgactcggc 20160

gacccgatcg aggcgcgggc gctgttcgag gcgtacgggc gcgaccgcga gcagccgctg 20220

cacctgggct cggtcaagtc caacctcggc cacacccagg cggccgccgg tgttgccggc 20280

gtgatcaaga tggtgctggc gatgcgcgcg ggcacccttc cccgcactct gcacgcatcg 20340

gagcggtcga aggagatcga ctggtcatcc ggtgcgatca gcctgctcga cgagccggag 20400

ccgtggcccg ccggcgcgcg accgcgccgg gcgggggtct cgtcgttcgg catcagcggc 20460

accaacgcgc acgccatcat cgaggaagct ccgcaggtcg tcgaaggcga gcgggtcgag 20520

gccggcgacg tcgtggcgcc ctgggtgctt tcggcgagca gcgcggaagg tctgcgcgcc 20580

caggcggcgc ggctggccgc gcacctgcgc gagcaccccg gtcaggaccc gcgcgacatc 20640

gcgtactcgc tcgcgacggg acgggccgcg ctgccccacc gcgccgcctt cgcccccgtc 20700

gacgagtccg ccgcgctgcg cgtgctcgac ggtctcgcga cgggaaacgc cgacggtgcc 20760

gccgttggaa cgagccgggc gcagcagcgc gccgtcttcg tcttccccgg gcagggttgg 20820

cagtgggcgg gcatggccgt cgacctgctc gacacctccc cggttttcgc agccgcgttg 20880

cgcgagtgcg ccgacgcgct cgaaccgcat ctggacttcg aggtgatccc gttcctgcgc 20940

gcggaagccg cgaggcggga gcaggacgcg gcgctgtcga ccgagcgcgt ggacgtggtg 21000

cagcccgtga tgttcgcggt catggtctcg ctggcgtcga tgtggcgagc ccacggcgtc 21060

gagccggccg cggtcatcgg gcactcccag ggcgagatcg ccgccgcgtg cgtcgcgggc 21120

gcgctctcgc tggacgacgc cgcgcgcgtg gtcgcgctgc gcagccgcgt catcgccacc 21180

atgcccggga acaagggcat ggcctcgatc gccgctccgg ccggcgaagt ccgcgcgcga 21240

atcggtgacc gcgtcgagat cgccgccgtc aacggtccgc gctcggtggt ggtcgccggc 21300

gacagcgacg aactggaccg gctggtcgct tcctgcacca ccgagtgcat ccgcgccaag 21360

cggctggccg tggactacgc gtcgcactcc tcgcacgtcg agacgatccg agacgcactg 21420

cacgccgagc tgggagagga cttccacccg ctgccggggt tcgtgccctt cttctccacc 21480

gtcaccgggc gctggacgca gccggacgag ctcgacgccg ggtactggta ccggaacctg 21540

cgccgcaccg tgcggttcgc ggacgccgtc cgtgcgctcg ccgagcaggg atatcgcacg 21600

ttcctggagg tcagcgcgca cccgatcctc accgccgcga tcgaggagat cggcgacgga 21660

tcgggcgccg acctctccgc catccattcg ctgcgccgcg gtgacggcag cctcgcggac 21720

ttcggcgaag cgctctcccg cgcgttcgcc gccggtgtcg cggtggactg ggagtcggtg 21780

cacctgggca ccggagcacg ccgggtgccc ttgcccacct acccgttcca gcgcgagcgc 21840

gtctggctcg aaccgaagcc ggtggcgcgc cggtccaccg aggtcgacga ggtttccgcg 21900

ctgcgctacc gcatcgagtg gcggcccacc ggtgccggtg aacccgcccg gctcgacggc 21960

acctggctgg tggcgaagta cgccggaacc gcggacgaga cgagcaccgc ggctcgggag 22020

gccctggagt cggccggggc gcgggtccgc gaactggtcg tggacgcccg ctgcggtcgc 22080

gacgaactcg cggagcggct tcgttcggtc ggcgaggtgg caggagtgct gtccctgctc 22140

gcggtggacg aagcggagcc ggaggaggcg ccgctcgcgc tggcttcgct ggcggacacg 22200

ctcagcctcg tgcaggcgat ggtgtcggcc gaactcggat gtccgctgtg gacggtgacg 22260

gaaagcgccg tcgcgacggg gccgttcgaa cgcgtccgca acgccgccca cggcgccctg 22320

tggggcgtcg ggcgggtcat cgcgctggag aaccccgccg tgtggggcgg cctggtcgac 22380

gtgcccgcgg ggtcggtcgc cgagctggcc cggcacctcg cggcggtcgt gtccggcggc 22440

gccggtgagg accagctcgc gctgcgcgcc gacggggtgt acggacgccg gtgggtgcgc 22500

gcggcggccc cggcgaccga tgacgagtgg aaacccaccg gaaccgtgct ggtcaccggt 22560

ggcacgggcg gtgtcggcgg gcagatcgcg cgctggctcg cccggcgggg cgcgccccac 22620

ctgctgctgg tgagccgcag cgggccggac gcggacggcg ccggcgaact ggtcgccgag 22680

ctcgaggcgc tgggcgcccg gacgaccgtc gcggcctgcg acgtgaccga ccgcgagtcg 22740

gttcgcgagc tgctcggcgg catcggtgac gacgtcccgc tctcggcggt gttccacgcc 22800

gccgccacgc tcgacgacgg caccgtggac accctcaccg gcgagcgcat cgagcgggca 22860

agtcgcgcca aggtgctcgg cgcgcgcaac ctgcacgagc tgacgcgcga gctggacctg 22920

accgccttcg tgctgttctc gtccttcgcc tcggccttcg gcgcccccgg gctcggcggc 22980

tacgcgcccg gcaacgccta cctcgacggc ctcgcccagc agcggcggag cgacggactc 23040

cccgcgaccg ccgtggcgtg ggggacgtgg gcgggcagcg ggatggccga aggcccggtg 23100

gccgaccgct tccgcaggca cggcgtcatc gagatgcctc ccgagacggc ctgccgggcg 23160

ttgcagaacg cgctggaccg cgccgaggtc tgcccgatcg tcatcgacgt caggtgggac 23220

cggttcctgc tcgcctacac cgcgcagcgg ccgaccaggc tcttcgacga gatcgacgac 23280

gcgcggcggg ctgcgccgca ggcggcggcc gaaccgcggg tgggcgcgct ggcgtcgctg 23340

cccgcgccgg agcgcgagaa agcgctgttc gagctcgtgc gctcgcacgc ggccgccgtc 23400

ctcggccacg cctcggccga gcgggtgccc gccgaccagg ccttcgcgga actcggcgtc 23460

gactcgctgt cggcgcttga gctgcgcaac cggctcggcg ccgcgaccgg tgtccgcctg 23520

ccgacgacga ccgtcttcga ccaccccgac gtgcggacgc tggcggcgca cctggccgcc 23580

gaactcggcg gtgcgaccgg agccgagcag gcggcaccgg cgaccacggc ccccgtcgac 23640

gagccgatcg cgatcgtcgg catggcgtgc cggctgcccg gggaggtcga ctccccggag 23700

cggctgtggg agctgatcac ctcgggacgc gactccgcgg cggaggtccc cgatgaccgg 23760

ggctgggtcc ccgacgagct gatggcctcc gacgcggcgg gaacccgccg cgcccacggc 23820

aacttcatgg cgggcgccgg tgacttcgac gcggcgttct tcgggatctc gccgcgcgag 23880

gcgctggcga tggacccgca gcagcgccag gcgctggaga cgacgtggga ggcgctggaa 23940

agcgcgggca tcccaccgga gacgttgcgc ggcagcgaca ccggcgtgtt cgtcggcatg 24000

tcccaccagg gctacgcgac cgggcgtccg cgcccggagg acggcgtcga cgggtacctg 24060

ctcaccggca acaccgcgag cgtcgcgtcg ggacgcatcg cctacgtgct ggggctggaa 24120

ggtcccgcgc tgacggtgga cacggcgtgt tcgtcgtcgt tggtggcgtt gcacacggcg 24180

tgtgggtcgt tgcgtgacgg tgactgcggt cttgcggtgg ccggtggtgt gtcggtgatg 24240

gcgggtccgg aggtgttcac cgagttctcc cgccagggcg cgctctcgcc ggacggccgg 24300

tgcaagccct tctcggacga ggccgacgga ttcggtctcg gggagggttc ggcgttcgtc 24360

gtgctccagc ggttgtccga cgccaggcgg gagggccgcc gcgtgctcgg cgtggtggcc 24420

gggtccgcgg tgaaccagga cggcgcgagc aacgggctct ccgctccgag cggcgtcgcg 24480

cagcagcggg tcatccgccg ggcgtgggcg cgtgcgggga tcacgggcgc ggatgtggcc 24540

gtggtggagg cgcatgggac cggtacgcgg ctgggcgatc cggtggaggc gtcggcgttg 24600

ctggctactt acggcaagtc gcgcgggtcg tcgggcccgg tgctgctggg ttcggtgaag 24660

tcgaacatcg gtcacgcgca ggcggccgcg ggtgtcgcgg gcgtgatcaa ggtgctgctc 24720

ggcctggaac gcggtgtggt gcccccgatg ctgtgccggg gcgagaggtc gggcctcatc 24780

gactggtcct ccggcgagat cgagctcgca gacggcgtgc gggagtggtc gcccgccgcg 24840

gacggggtgc gccgggcagg tgtgtcggcg ttcggggtga gcgggacgaa cgcgcacgtg 24900

atcatcgccg agccgccgga accggagccc gtgccgcaac cgcgacgcat gctgcccgcg 24960

accggggtgg tgccggtcgt gctgtcggcc aggaccgggg cggcgttgcg ggcgcaggcc 25020

ggcaggctcg ccgaccacct cgccgcgcat cccgggatcg caccggccga cgtgagctgg 25080

acgatggcgc gggcccgcca gcacttcgag gagcgggccg cggtgctcgc cgccgacacc 25140

gccgaggccg tgcaccggtt gcgggcggtg gccgacggcg cggtggttcc cggtgttgtc 25200

accggcagtg cctccgacgg tggttcagtg ttcgtcttcc ctgggcaggg tgcccagtgg 25260

gaaggcatgg cgcgggagtt gttgccggtt cccgtcttcg ccgagtcgat cgccgagtgc 25320

gatgcggtgt tgtcggaggt ggccggattc tcggtgtccg aggtgctgga gccacgtccg 25380

gacgcgccgt cgctggagcg ggtcgacgtg gtgcagccgg tgctgttcgc ggtgatggtg 25440

tcgctggcgc ggttgtggcg tgcctgcggt gccgttcctt cggccgtcat agggcactcg 25500

cagggtgaga tcgccgccgc ggtggtggcg ggagcgttgt cgctggagga cggcatgcgc 25560

gtcgtcgccc gccggtcgag ggcggtgcgt gcggtcgcgg gccgggggag catgctctcg 25620

gtgcgcggcg gccgctccga cgtcgagaag ctgctcgccg acgacagctg gaccggcagg 25680

ctggaggtcg ccgcggtcaa cggccccgac gccgtggtgg tggccggtga cgcccaggcg 25740

gcgcgcgagt tcctggagta ctgcgagggc gtgggcatcc gcgcccgcgc gatcccggtg 25800

gactacgcct cgcacaccgc gcacgtcgag cccgtgcgcg acgaactggt ccaggcgctg 25860

gccgggatca ccccgcgacg ggccgaggtg ccgttcttct ccaccctgac cggcgacttc 25920

ctcgacggca ccgagctgga cgcgggctac tggtaccgca acctgcgtca cccggtggag 25980

ttccactccg ccgtgcaggc gctgaccgac cagggatacg cgacgttcat cgaggtcagc 26040

ccgcacccgg tgctggcgtc gagcgtccag gagaccctcg acgacgccga gtcggacgcg 26100

gcggtgctcg ggacgctgga acgcgacgcg ggcgacgccg accgcttcct cacggcactc 26160

gccgacgcgc acacgcgcgg tgtcgcggtc gactgggaag cggtgctcgg ccgggccgga 26220

ctggtcgacc tgccgggtta tcctttccag ggcaagcggt tctggctgct gccggaccgc 26280

accacccctc gtgacgagct cgacggctgg ttctaccggg tcgactggac cgaggtgccg 26340

cgctccgaac ctgccgcgct gcgcggccgt tggctcgtgg tggtgcccga ggggcacgag 26400

gaggacggct ggaccgtcga ggtgcggtcc gcgctcgccg aggccggcgc cgaaccggag 26460

gtcacgcgcg gcgtcggcgg gctggtcggt gactgcgcgg gcgtggtgtc gttgctcgcc 26520

ctcgagggcg atggtgcggt gcaaaccctt gtgctggtgc gggaactcga cgccgagggc 26580

atcgacgcgc cactgtggac ggtcaccttc ggcgcggtcg acgcgggcag tccggtggcc 26640

cgcccggacc aggcgaagct gtgggggctg ggccaggtcg cgtccctgga acgcgggccc 26700

cgctggaccg gcctcgtcga cctgccgcac atgccggacc cggaactgcg aggccgtctc 26760

accgcggtgc tggccggctc ggaggaccag gtcgcggtgc gcgccgacgc cgtgcgtgcg 26820

cggcggcttt cccccgccca cgtcaccgcc acctcggagt acgcggtgcc gggcggcaca 26880

atcctggtca ccggtggcac cgccggcctg ggcgcggagg tggcccggtg gctcgccggt 26940

cgcggcgccg aacacctcgc gctggtcagc aggcgaggcc cggacaccga gggcgtcggc 27000

gacctgaccg ccgagctgac ccggctcggc gcgcgggtgt cggtgcacgc gtgcgacgtc 27060

agcagccgcg aaccggtgag ggaactcgtg cacggcctga tcgagcaggg cgacgtcgtc 27120

cgcggtgtgg tgcacgcggc gggactgccg cagcaggtcg cgatcaacga catggacgag 27180

gccgccttcg acgaggtggt cgcggccaag gccgggggcg cggtgcacct ggacgagctg 27240

tgctcggacg ccgagctgtt cctgctgttc tcctccgggg ccggggtgtg gggaagcgcc 27300

cgccagggcg cctacgccgc gggcaacgcg ttcctggacg ccttcgcccg gcaccgccgg 27360

ggccgcggcc tgcccgccac gtcggtggcg tgggggctgt gggcggcggg cggcatgacc 27420

ggcgacgagg aggccgtgtc gttcctgcgc gagcgcggtg tgcgggcgat gcccgtaccg 27480

cgcgccctcg ccgccctgga cagggtgctg gcctccgggg agacggcggt ggtcgtgacg 27540

gacgtggact ggcccgcctt cgccgagtcc tacaccgccg cccggccccg gccgttgctc 27600

gaccgcatcg tcacgaccgc gccgagcgag cgggccggag aaccggagac ggagagcctg 27660

cgcgaccggc tggcgggtct gccgcgtgcc gagcggacgg cggagctggt gcgcctggtc 27720

cgcaccagca ccgcgaccgt gctgggccac gacgacccga aggcggtgcg cgcgaccacg 27780

ccgttcaagg agctcgggtt cgactcgctg gcggccgtcc ggctgcgcaa cctgctcaac 27840

gcggccaccg ggctccgcct gccgtcgacg ctggtcttcg accacccgaa cgcctccgcg 27900

gtcgccggtt tcctcgacgc cgagctcggc accgaggtcc ggggggaggc gccgtcggcc 27960

ctcgccgggc tggacgcgct ggaagcggcc ctgcccgagg tgcccgcaac cgagcgggaa 28020

gagctggtac agcgcttgga acggatgctc gccgcgctac gcccggtcgc ccaggccgcc 28080

gacgcctccg ggaccggcgc caacccgtcc ggcgacgacc tgggcgaggc gggcgtggac 28140

gaactgctcg aagcactcgg ccgggagctc gacggcgatt gaggacccct accccgcagc 28200

cgcagaaggg accgaggaac tttgacttct tccctgccct aaagggcggg gattgccatc 28260

gggctggcgc ccgatggttc ctgcttcacc gccgactgcc gaagggagga cccttgcgga 28320

ctcacgtcag ctccacaggc atcgccgggg gtcttcccgg ctaccgctcg accagcggca 28380

aggatgttct tcgccgcgtt gatgtcccgg tcgtgccggg tgcggcatcc cgggcacgtc 28440

catgcccggg tggaaaggct caacttctcc agcaggtgcc cgcaggccga gcacgtcttg 28500

ctggacgggt accagcggtc gatgacgacc agcgtcttgc ccgcgcgggt ggtcttgtac 28560

tccagttgcc gccggaactc gcccaacgcc gcgtccatca cggcacggtt caatccggat 28620

ttggcgcgca cgttacggcc aggcttggcc acggtgccct tcgcggagcg ggccattccg 28680

gcgatgttga ggtcttcgat cgcgatggcg tcgtgctcgc gcaccaggtt cgtggtggtg 28740

cggtgcagga agtcggtgcg ggcacaccgg atttcgcggt gtgcccgtgc gaccttggtc 28800

ttggctttgc ggcggttgtt cgagcccttc tgcttgcggg ccatttgccg ctggtagcgg 28860

gcgaggttgc gggccttgcg gtccagatgc ttcgggttgg cgatcttctc gcctgtcgac 28920

aggacagcaa agtccttcac acccaggtcc accccgaccg cgctgccggt ctcgggctgc 28980

ggttcggggt cgtcggtgtc cacggccaag gtcacatacc agcggccatc cggctcgcgg 29040

gacacgatca ccatcgtcgg attcagcgtt gcgagggttt cgtcgtccca ggaccacacg 29100

aactcaagcg gcgcggtgtg cttcgccaga gtcagcttgc cgccgcgcag gcggaaagcg 29160

ctgcgcgtgt agtgcgccga ctggcggccg ttgcggggct tgaaacgcgg gtagcgcgca 29220

cgcccggcga agaagttcgc atacgcggtg tgctggtgcc gaagtgtctg ctgcaacggc 29280

acgctcgaca cctcagacag gaacgccggc tcgtcggtct tcttccatgc cgtcagcgcg 29340

gcatcggcct ccctacagga ggtctttctg ccgtgcgcgt ggtaggcgcg gtgccgctcg 29400

gcgagggtct tgttccacac caggcgcacg cacccgaagg tgcggcccaa ctgggccgcc 29460

tgttcggggg ccgggtaagc ccgaaccctg tacgccgtcc tcacaaagat cattcaaccc 29520

gattgccgat cacgagtaag cgcgggtacg tggtcactga ggagcgctcg ggatcggctc 29580

cttccctgcg tgaacgccgg cgcttccgcc gattggagaa aaggtgactg acagcgagaa 29640

ggtggcggag tacctccgtc gggcgacgct cgacctgcgt gccgcccggc agcgcatccg 29700

cgagctggaa tccgacccga tcgccatcgt cagcatggcc tgccgcctgc cgggcggggt 29760

gaacaccccg cagcggctgt gggagctgct gcgcgagggc ggtgagacgc tgtcgggctt 29820

ccccaccgac cggggctggg acctggcgcg gctgcaccac cccgacccgg acaaccccgg 29880

taccagctac gtcgacaagg gcgggttcct cgacgacgcg gcgggcttcg acgcggagtt 29940

cttcggcgtc tcgccgcgcg aggccgcggc catggacccg cagcagcggc tgctgctgga 30000

gacgagctgg gagctggtgg agaacgccgg catcgacccg cactcgctgc gcggtaccgc 30060

gaccggcgtc ttcctcggag tggcgaagtt cggctacggc gaggacaccg ccgcggcgga 30120

ggacgtcgag ggctactcgg tcaccggtgt ggcgcccgcg gtcgcctccg gccgcatctc 30180

ctacaccatg ggcctggagg ggccgtcgat cagcgtcgac accgcgtgct cgtcgtcgct 30240

ggtggcgctg cacctggcgg tcgagtcgct gcgcaagggc gagtcgtcga tggcggtcgt 30300

cggcggtgcc gcggtgatgg cgaccccggg ggtgttcgtc gacttcagcc ggcagcgcgc 30360

gctcgccgcc gacgggcggt cgaaggcgtt cggtgccggc gccgacgggt tcggcttctc 30420

cgaaggcgtc accctggtcc tgctcgagcg gctgtcggag gcgcggcgaa acgggcacga 30480

ggtgctggcg gtggttcgcg gctcggcgct caaccaggac ggggccagca acgggctttc 30540

cgcgccgagc gggcccgcgc agcgcagggt catccggcag gccctcgaga gctgcggtct 30600

ggagcccggc gacgtcgacg cggtggaggc gcacggcacc ggtacggcgc tcggcgaccc 30660

gatcgaggcg aacgcgctgc tggacaccta cggccgcgac cgcgacgccg accggccgct 30720

ctggctgggc tcggtgaagt ccaacatcgg ccacacccag gcggcagcgg gcgtcaccgg 30780

cctgctgaag gtggtcctgg cgctgcgcaa cggggaactg cccgcgaccc tgcacgtcga 30840

ggagcccacg ccgcacgtcg actggtcgtc cggcggcgtg gcgctgctgg cgggcaacca 30900

gccgtggcgg cgcggcgagc ggactcggcg cgccgctgtt tccgcgttcg ggatcagcgg 30960

gacgaatgcg cacgtgatcg tcgaggaagc tcctgagcgc gagcaccggg agaccaccgc 31020

gcacgacggc cgaccggttc cgctggtggt gtccgcgcgc agcacggcgg cgttgcgggc 31080

gcaggccgcc cagatcgccg agctgctcga acgcccggac gccgacctcg ccggggtcgg 31140

gctgggcctg gccacgaccc gcgcccgcca cgagcaccgc gccgccgtgg tggcatcgac 31200

ccgcgaggaa gcggtgcgcg gactgcggga gatcgccgcc ggtgccgcga cggccgacgc 31260

cgtggtcgag ggcgtcaccg aggtggacgg gcgcaacgtc gtcttcctgt tcccggggca 31320

gggttcgcaa tgggccggca tgggtgccga gctgctgtcg tcgtcgccgg tgttcgccgg 31380

gaagatccgg gcctgcgacg agtcgatggc cccgatgcag gactggaagg tctccgacgt 31440

gctgcgtcag gcgccggggg cgccgggcct ggaccgggtc gacgtggtgc agccggtgtt 31500

gttcgcggtg atggtgtcgc tggcggagct gtggcgctcg tacggcgtgg agcccgcggc 31560

ggtcgtgggg cactcgcagg gcgagatcgc cgccgcgcac gtcgccgggg cgctcacgtt 31620

ggaggacgcg gcgaagctcg tcgtgggccg cagccgcctg atgcggtcgc tctccgggga 31680

gggcggcatg gccgccgtcg cgctgggcga ggccgcggtg cgcgagcgcc tgcggccgtg 31740

gcaggaccgg ctctcggtgg ccgcggtcaa cggtccccgg tcggtcgtgg tctccggcga 31800

gcccggcgcg ctgcgggcgt tttccgagga ctgcgcggcc gagggcatcc gcgtccgcga 31860

catcgacgtg gactacgcct cgcactcgcc gcagatcgag cgggtccgcg aggaactcct 31920

cgaaacgacc ggcgacatcg cgccgcgccc ggcgcgggtg acgttccact ccactgtgga 31980

gtcgcggtct atggacggca ccgagctgga tgcccggtac tggtaccgca acctgcgcga 32040

gacggtgcgc ttcgccgacg ccgtgacgcg gctggcggag tcgggatacg acgcgttcat 32100

cgaggtcagc ccgcatccgg tcgtggtcca ggccgtcgag gaggcggtcg aagaggctga 32160

cggtgccgaa gacgcggtcg tagtcggctc gctgcaccgc gacggcggtg acctctcggc 32220

cttcctgcgg tcgatggcca ccgcgcacgt gtccggtgtg gacatcaggt gggacgtcgc 32280

tctgcccggc gccgcgccct tcgcgctgcc gacgtatccg ttccagcgca agcgctactg 32340

gctccagccc gccgcacccg ccgccgcctc cgacgagctg gcctaccgcg tttcctggac 32400

tccgatcgaa aagccggagt cgggaaacct ggacggcgac tggttggttg tcacacccct 32460

catcagtccg gagtggacgg aaatgctgtg cgaggccatc aacgccaacg gtggcagggc 32520

gttgcgctgc gaggtggaca cgtccgcttc gcgcactgag atggcccagg ccgtcgcaca 32580

ggccggaacg ggattccggg gcgtgctctc gttgctgtcg tcggacgaat ccgcctgccg 32640

tccgggggtt cctgccggtg cggtcggcct gctcaccctg gtccaggcgc tgggcgatgc 32700

cggggtcgac gcaccggtgt ggtgcctgac ccagggtgcg gtccgcactc ccgccgacga 32760

cgacctcgcc cggcctgcgc agaccaccgc gcacggcttc gcgcaggtcg ccgggctgga 32820

gctgccgggc cgctggggcg gtgtggtcga cctgcccgaa tcggtcgacg acgcggcgct 32880

gcgtctgctc gtggcagtcc tgcgcggcgg cggccgtgcc gaggaccacc tcgcggtccg 32940

ggacggccgc ctccacggcc gtcgcgtcgt ccgcgcaagc ctgccgcagt ccggctcgcg 33000

gagctggacc ccgcacggga ccgtgctggt caccggcgcg gcgagccccg tcggcgacca 33060

actggtgcgg tggctcgccg accggggagc cgagcggctg gtgctggccg gagcctgtcc 33120

gggcgacgac ctgctggccg cggtcgagga agcgggcgca tcggccgtcg tgtgcgccca 33180

ggacgcggcg gcgctgcgcg aggcgctcgg cgacgagccg gtgaccgcgc tcgtgcacgc 33240

cggaaccctg acgaacttcg gcagcatcag cgaagtcgca ccggaggagt tcgccgagac 33300

gatcgcggcc aagaccgcgt tgctcgccgt gctggacgaa gtcctcggcg accgggccgt 33360

cgagcgggag gtctactgct cgtcggtcgc cgggatctgg ggcggcgccg ggatggccgc 33420

ctacgcggca ggcagcgcct acctcgacgc gctggccgag caccaccgcg cgcggggccg 33480

ctcgtgcacc tcggtcgcct ggacgccgtg ggcgctgccg ggcggggcgg tggacgacgg 33540

ctacctgcgg gaacgcggac tgcgcagcct ctccgccgac agggcgatgc gcacctggga 33600

gcgggtgctg gccgccgggc cggtgtcggt cgcggtggcc gacgtggact ggccggtgct 33660

cagcgaaggc ttcgccgcca cccggccgac cgcgctgttc gccgaactcg ccggccgcgg 33720

cggacaggcg gaggccgagc cggacagcgg accgaccggc gagccggcac aacggctcgc 33780

ggggctttcc ccggacgagc agcaggaaaa cctgctcgaa ctcgtcgcga acgcggttgc 33840

cgaggtgctt ggccacgagt ccgccgccga gatcaacgtg cgccgcgcgt tcagcgagct 33900

cggactcgac tcgctcaacg cgatggccct gcgcaagcgc ctgtcggcga gcaccggcct 33960

gcggctgccc gcgtcgctgg tgttcgacca ccccaccgtc accgcgctcg cgcagcacct 34020

gcgcgcccgg ctcgtcggtg acgccgacca ggccgcggtg cgcgtcgtcg gcgcggccga 34080

cgagtccgag cccatcgcca tcgtcggcat cggctgccgt ttccccggcg gcatcggctc 34140

gcccgagcag ttgtggcggg tgctggccga gggcgcgaac ctcaccaccg gcttcccggc 34200

cgaccggggc tgggacatcg ggcggctcta ccacccggac ccggacaacc ccggcaccag 34260

ctacgtggac aagggcgggt tcctcaccga cgcggcggat ttcgacccgg gcttcttcgg 34320

catcacgccc cgcgaagcgc tggcgatgga cccgcagcag cgcctcatgc tggagacggc 34380

gtgggaggca gtggaacgcg cgggcatcga ccccgacgcc ctgcgaggca ccgacaccgg 34440

cgtcttcgtc ggcatgaacg gccagtccta catgcagctg ctggccggtg aggccgaacg 34500

cgtcgacggc taccagggcc tcggaaactc cgcgagcgtg ctctccgggc gcatcgccta 34560

caccttcggc tgggagggcc cggcgctgac ggtggacacc gcgtgctcgt cctcgctggt 34620

cggcatccac ctcgcgatgc aggcgctgcg gcgcggtgag tgctccctgg cgctggccgg 34680

cggcgtcacg gtcatgtccg acccgtacac cttcgtcgac ttcagcacgc agcgcgggct 34740

cgcctccgac ggtcgctgca aggcgttctc cgcgcgggcc gacggcttcg cgctgtcgga 34800

aggcgtcgcc gcgctggtgc tggagccgct ttcccgggcg cgcgccaacg ggcaccaggt 34860

gctggccgtg ctgcgcggca gcgcggtcaa ccaggacggt gccagcaacg gtctcgccgc 34920

tcccaacggc ccgtcgcagg agcgggtgat ccggcaggcg ctcgccgctt cgggcgtgcc 34980

ggccgcggac gtcgacgtcg tggaggcgca cgggacgggc accgagctcg gcgacccgat 35040

cgaggccggc gcgctcatcg cgacctacgg ccaggaccgc gaccggccgc tgcggctcgg 35100

ctcggtgaag accaacatcg gccacaccca ggccgcggcg ggcgccgcgg gcgtgatcaa 35160

ggtcgtgctg gcgatgcggc acgggatgct gccccggtcg ttgcacgccg acgagctgtc 35220

cccgcacatc gactgggagt cgggggccgt ggaggtgctg cgcgaggagg tgccgtggcc 35280

ggcgggtgag cgcccccggc gggcgggggt gtcgtccttc ggcgtcagcg gaaccaacgc 35340

gcacgtgatc gtcgaagagg caccagcaga gcaggaggcc gcccgcaccg agcgcggtcc 35400

gctgccgttc gtgctgtccg gccgcagcga agccgtggtc gcggcccagg cccgcgcgct 35460

cgccgagcac ctgcgcgaca ccccggagct cggcctgacc gacgcggcgt ggacgctcgc 35520

gaccggcagg gcgcggttcg acgtgcgagc cgccgtgctc ggcgacgacc gcgcgggcgt 35580

gtgcgcggag ctggacgcgc tggccgaggg ccgcccgtcg gccgacgccg tcgcgccggt 35640

gacctccgcg ccgcgcaagc cggtcctggt cttccccggc cagggcgcgc agtgggtcgg 35700

catggcacgc gatctgctgg aatcctccga ggtgttcgcc gagtcgatga gccggtgcgc 35760

cgaggcgctc tcgccgcaca ccgactggaa gttgctcgac gtcgtccgcg gcgacggcgg 35820

tcccgacccg cacgagcgcg tcgacgtgct ccagccggtg ctcttctcga tcatggtctc 35880

gctggccgag ctgtggcgcg cgcacggcgt gaccccggcc gccgtcgtcg gccactcgca 35940

gggcgagatc gccgcggcgc acgtggcggg cgcgctgtcg ctggaagccg ccgcgaaggt 36000

ggtggccctg cgcagccagg tgttgcgcga gctcgacgac cagggcggca tggtgtcggt 36060

cggcgcgtcc cgcgacgagc tggagaccgt gctcgcgcgc tgggacggcc gtgtcgcggt 36120

ggccgccgtg aacgggcctg gcaccagcgt cgttgccggg ccgaccgcgg agctggacga 36180

gttcttcgcc gaggccgagg cgcgggagat gaagccgcgc cggatcgccg tgcgctacgc 36240

ctcccactcc ccggaggtgg cgcgcatcga ggaccggctc gcggccgagc tgggcaccat 36300

caccgccgtg cggggctcgg tgccgctgca ctccacggtg accggcgagg tcatcgacac 36360

ctccgcgatg gacgcctcct actggtaccg caacctgcgc cgaccagtgc tcttcgagca 36420

ggcggtgcgc ggtctggtcg agcagggctt cgacaccttc gtcgaggtga gcccgcaccc 36480

ggtgctgctg atggcggtcg aggagaccgc cgagcacgcg ggcgcggaag tcacctgcgt 36540

gccgacgctg cgccgcgagc agagcggacc gcacgagttc ctgcgcaacc tgctgcgggc 36600

tcacgtgcac ggcgtcggcg ccgacctgcg tccggcggtg gccgggggac ggccggccga 36660

gctgcccacc tacccgttcg aacaccagcg cttctggccg cggccgcacc ggcccgccga 36720

cgtctcggcg ctgggcgtgc gcggcgcgga gcacccgctg ctgctcgccg cggtcgacgt 36780

gccgggccac ggcggtgcgg tgttcaccgg aaggctttcc accgacgagc agccgtggct 36840

ggccgaacac gtcgtgggcg gccggacgct ggtgccgggc agcgtcctgg tcgatctcgc 36900

gctcgccgcg ggtgaggacg tcgggctgcc ggtcctggag gaactggtgt tgcaacggcc 36960

gctggtgctg gccggggcgg gggcgctgct gcgcatgtcg gtcggcgcgc ccgacgagtc 37020

ggggcggcgg acgatcgacg tccacgccgc cgaagacgtg gccgacctcg ccgacgcgca 37080

gtggtcgcag cacgccaccg ggacgctcgc gcagggcgtc gccgcgggtc cgagggatac 37140

cgagcagtgg ccgccggagg acgccgtccg catcccgctc gacgaccact acgacggcct 37200

cgccgagcag ggctacgagt acggaccgtc gttccaggcc ctgcgagccg cgtggcgcaa 37260

ggacgactcg gtctacgccg aggtgtccat cgcggcggac gaggaaggtt acgcgttcca 37320

cccggtgctg ctcgacgccg tggcgcagac gctcagcctg ggcgccctcg gcgagccggg 37380

cgggggaaag ctgccgttcg cgtggaacac cgtgaccctg cacgcctccg gggcgacctc 37440

ggtgcgggtc gtggcgacgc ccgccggggc ggacgcgatg gccctgcggg tcaccgaccc 37500

ggcaggccac ctggtcgcca cggtcgactc gctggtcgtc cgcagcaccg gggagaagtg 37560

ggagcagccc gaaccgcgcg gtggcgaggg cgagctgcac gctctggact gggtacggct 37620

agccgagccc ggctcgaccg gtcgtgtggt cgcggccgat gcctcggacc tcgacgccgt 37680

cctgcggtcc ggtgaacccg aacccgacgc ggtcctggtc cgctacgaac ccgaaggcga 37740

cgacccccgc gccgcggccc gccacggcgt cctctgggcc gccgcgctcg tgcgccgctg 37800

gctcgaacag gaggagctgc cgggcgcgac gctggtcatc gccacgtccg gcgcggtcac 37860

cgtgtccgac gacgacagcg ttcccgaacc cggcgccgcc gcgatgtggg gcgtgatccg 37920

ctgtgcgcag gccgagtcgc cggaccggtt cgtgctcctc gacaccgacg cggaacctgg 37980

gatgctgcct gcggttccgg acaacccgca gctcgcgttg cgcggcgacg acgtcttcgt 38040

gccgcgcctc tcgccgctcg caccttccgc gctgacgctt ccggcaggca cccaacgtct 38100

cgtgccgggt gacggggcga tcgactccgt ggccttcgag cccgcacccg acgtcgagca 38160

gccgctccgg gcgggcgagg tccgggtgga cgtgcgcgcc accggagtca acttccgcga 38220

cgtcctcctc gcactcggca tgtatccgca gaaggcggac atgggcaccg aggccgccgg 38280

tgtcgtcacg gcggtcggac cggacgtgga cgccttcgcg ccgggagacc gggtgctcgg 38340

cctgttccag ggagccttcg cgccgatcgc ggtcaccgat caccggctcc tcgcacgagt 38400

gccggacggc tggagcgacg ccgacgccgc ggccgtgccc atcgcctaca ccacggcgca 38460

ttacgcgctg cacgatctcg cggggctgcg cgcgggtcag tcggtgctca tccacgcagc 38520

ggcaggcggt gtcggcatgg cggccgtcgc gctggcccgc cgagcggggg cggaggtgtt 38580

ggccaccgcc ggcccggcca agcacgggac gctgcgggcg ctcggtctcg acgacgagca 38640

catcgcttcc tcccgggaga ccggtttcgc ccggaagttc cgggagcgca ccggaggccg 38700

cggcgtggac gtggtgctca actcgctcac cggggaactg ctcgacgagt ccgcggatct 38760

gctcgccgag gacggcgtct tcgtcgagat gggcaagacc gacctgcggg acgccgggga 38820

cttccggggc cgatacgccc cgttcgacct cggcgaggcg ggtgacgacc ggctcgggga 38880

gatcctgcgc gaggtcgtcg gcctgctggg cgccggggag ctcgaccggc tcccggtatc 38940

ggcgtgggag ctgggatccg cgcccgcggc gttgcagcac atgagccggg gcaggcacgt 39000

cggcaagctc gtgctgaccc agcccgcgcc ggtggacccg gacggcacgg tgctgatcac 39060

gggtggcacc ggcacgctcg gacggctgct cgcgcgccac ctcgtcaccg agcacggcgt 39120

gcggcacctg ctgctggtca gcaggcgcgg cgcggacgcg ccgggttccg acgagctgcg 39180

cgcggagatc gaggacttgg gcgcgtccgc ggagatcgcg gcttgcgaca ccgccgaccg 39240

cgacgcgctt tcggcgctgc tggacgggct gccccggccg ctgaccggtg tcgtgcacgc 39300

ggcgggtgtg ctggccgacg ggctggtcac ctccatcgac gagccggcgg tggagcaggt 39360

gctgcgcgcc aaggtcgacg cggcgtggaa cctgcacgag ctgaccgcga acaccggtct 39420

gagcttcttc gtgctgttct cgtccgcggc gtcggtgcta gccggcccgg ggcagggcgt 39480

gtacgcggcc gcgaacgagt cgctcaacgc gctggctgcc ctccggagga cgcgcggcct 39540

tcccgcgaag gcgctcggat ggggactgtg ggcgcaggcc agcgagatga ccagcggact 39600

cggcgaccgc atcgcccgga ccggggtcgc cgcgctgccg accgagcggg cgctcgcact 39660

gttcgacagc gccctgcgcc gcggcggtga ggtcgtgttc ccgctgtcca tcaaccgttc 39720

cgcgctgcgc agggccgagt tcgtgccgga ggtcctgcgc ggcatggtca gggcgaagct 39780

gcgcgccgcc gggcaggccg aggcggcagg gccgaacgtg gtcgaccggc tcgccggtcg 39840

gtccgagtcc gaccaggtcg ccgggctggc cgaactggtg cgttcacacg cggcggcggt 39900

ctccgggtac ggctcggccg accagctccc cgagcgcaag gcgttcaagg acctcggttt 39960

cgactcgctg gccgcggtgg agctgcgcaa ccgcctcggt accgcgaccg gcgtgcggct 40020

gcccagcacg ttggtgttcg accacccgac tccgctggcg gtggccgaac acctgcggga 40080

caggctgttc gcggcctcac cggcggtgga catcggcgac cggctggacg agctggagaa 40140

ggcgctcgaa gccctgtccg ccgaggacgg gcacgacgac gtgggccagc gcctggagtc 40200

gctgctgcgc cggtggaaca gcaggcgggc ggacgccccg agcacgtccg cgatcagcga 40260

ggacgccagt gacgacgagc tgttctcgat gctcgaccag cggttcggcg ggggagagga 40320

cctgtagatg agcggtgaca acggcatgac cgaggaaaag ctccggcgct acctcaagcg 40380

caccgtcacc gagctcgact cggtgaccgc gcgcctgcgt gaagtcgagc accgggccgg 40440

tgagccgatc gcgatcgtcg gcatggcgtg ccggttcccc ggcgacgtgg actcgccgga 40500

gtcgttctgg gagttcgtgt ccggcggcgg ggacgccatc gcggaggccc ccgccgaccg 40560

cggctgggag ccggaccccg acgcgcggct gggcgggatg ctcgcggccg cgggcgactt 40620

cgacgcgggc ttcttcggga tctcgccgcg cgaggcgctg gcgatggacc cgcagcagcg 40680

gatcatgctg gagatctcgt gggaggcgct ggagcgcgcc ggccacgatc cggtgtccct 40740

gcgcggcagc gcgaccgggg tgttcaccgg tgtcggcacc gtggactacg gcccgcgacc 40800

cgacgaggcc ccggacgagg tcctgggcta cgtcggcacc ggcaccgcct ccagcgtcgc 40860

ctccggccgg gtcgcctact gcctgggcct ggaaggcccg gcgatgaccg tcgacaccgc 40920

ctgttcctcc gggctcaccg ccctgcacct ggcgatggag tcgctgcgcc gggacgagtg 40980

cggcctggcg ctggccggcg gcgtgacggt gatgagcagt cccggggcgt tcaccgagtt 41040

ccgcagccag ggcgggctcg ccgccgacgg ccgctgcaag ccgttctcga aggccgccga 41100

cgggttcggc ctggccgagg gtgccggggt cctggtgctg caacggctgt cggccgcgcg 41160

gcgggagggc agaccggtgc tggccgtgct gcggggctcg gcggtcaacc aggacggcgc 41220

cagcaacggg ctgaccgcgc cgagcggacc cgcgcagcag cgggtcatcc gccgggcgct 41280

ggagaacgcc ggtgtccggg cgggcgacgt cgactacgtg gaggcccacg gcaccggcac 41340

caggctgggc gaccccatcg aggtgcacgc gctgctctcg acctacggcg cggaacgcga 41400

cccggacgat ccactgtgga tcggttcggt caagtccaac atcggccaca cccaggccgc 41460

cgccggcgtc gccggggtga tgaaggcggt gctggcgctg cggcacggcg agatgccgcg 41520

cacgctgcac ttcgacgagc cctcgccgca gatcgagtgg gacctgggcg cggtgtcggt 41580

ggtgtcgcag gcgcggtcgt ggcccgccgg cgagaggccc cgcagggcgg gcgtctcctc 41640

gttcggcatc agcggcacca acgcgcacgt catcgtcgaa gaggcgcccg aggccgacga 41700

gcccgagccg gcacccgact cgggtccggt cccgctggtg ttgtccggcc gcgacgagca 41760

ggcgatgcgg gcgcaggcgg gacggctggc agaccacctc gcccgcgagc cgcggaactc 41820

gttgcgcgac accggtttca cgctggccac ccgccgcagc gcgtgggagc accgcgcggt 41880

ggtggtcggc gaccgcgacg acgccctcgc cgggctgcgc gcggtggccg acggccgcat 41940

cgccgaccgg acggccaccg ggcaggcccg aactcgccgc ggcgtcgcga tggtgttccc 42000

cggccagggc gcgcagtggc aggggatggc ccgcgacctg ctgcgggagt cgcaggtatt 42060

cgccgactcg atccgcgact gcgagcgggc gctggccccg cacgtcgact ggtcgctgac 42120

cgacctgctc agcggcgcgc gaccgctgga ccgggtcgac gtcgtccagc ccgcgctctt 42180

cgccgtcatg gtgtcgctgg cggcgctgtg gcgctcccac ggcgtcgagc ccgccgcggt 42240

cgtcggccac tcgcagggcg agatcgccgc cgcgcacgtc gccggcgcgc tcaccctgga 42300

ggacgccgcc aagctcgtcg cggtccggag ccgggtcctg cgccggctcg gcggccaggg 42360

cggcatggcg tcgttcgggc tgggcaccga gcaggcggcc gaacggatcg ggcgcttcgc 42420

gggcgcgctc tccatcgcct cggtcaacgg cccccggtcg gtcgtcgtcg cgggggagag 42480

cgggccgctg gacgagctga tcgccgagtg cgaggccgaa ggcataacgg cgcgccgcat 42540

ccccgtcgac tacgcctccc actcaccgca ggtggagtcg ctgcgcgagg agctgctgac 42600

cgagctggcg ggcatctccc cggtgtcggc ggacgtggcg ctctactcga ccacgaccgg 42660

gcagcccatc gacaccgcca cgatggacac cgcctactgg tacgcgaacc tgcgcgagca 42720

ggtccgcttc caggacgcga cgcggcagct cgccgaggcg gggttcgacg cgttcgtcga 42780

ggtcagcccg catccggtgc tgaccgtcgg catcgaggcc acgctggact ccgcgctccc 42840

ggccgacgcc ggcgcctgcg tcgtgggcac cctgcggcgg gaccgcggcg gcctggccga 42900

cttccacacc gcgctcggcg aggcgtacgc gcagggcgtg gaggtcgact ggagccccgc 42960

cttcgccgac gcgcggccgg tcgagctgcc cgtctacccg ttccagcggc agcggtactg 43020

gctgcccatc cccaccggcg ggcgcgcacg ggacgaggac gacgactggc gctaccaggt 43080

cgtatggcgg gaagccgagt gggagagcgc ttcgctggcc ggacgcgtgc tgctggtgac 43140

cggaccgggc gtgccgtccg agttgtcgga cgccatccga agtggactgg agcagagcgg 43200

tgcgacggtc ctgacctgcg acgtggaatc ccgttcgacc atcggcaccg cactggaggc 43260

cgccgacacc gacgctctgt ccactgtggt gtcgctgctg tcccgcgacg gcgaggccgt 43320

cgatccgtcg ctggacgcgc tcgccctggt ccaggccctc ggagcggccg gggtcgaagc 43380

accgctgtgg gtgctgaccc gcaacgccgt gcaggtggcc gacggcgaac tggtcgatcc 43440

ggcgcaggcc atggtgggcg gtctcggccg cgtggtcggc atcgagcagc cggggcgctg 43500

gggcggtctg gtggacctgg tcgacgccga tgccgcgtcg atccggtcgc tggccgcggt 43560

gctggcggac ccgcgcggcg aggagcaggt cgcgatccgg gcggacggga tcaaggtggc 43620

gaggctcgtg cccgcccccg cccgcgccgc acgcacccgc tggagccctc gcggcaccgt 43680

gctggtcacc ggcggcaccg gagggatcgg cgcgcacgtc gcccgctggc tggcccgctc 43740

gggcgccgag cacctggtgc tgctgggcag gcgcggtgcc gacgcacccg gcgcgtccga 43800

gctgagggag gagctgaccg cgctcggcac gggcgtgacc atcgccgcct gcgacgtcgc 43860

cgaccgggcg cggctcgaag cggtgctcgc cgcggagcgc gccgagggac gcacggtcag 43920

cgccgtgatg cacgcggcgg gggtttccac gtccacgccc ctcgacgacc tcaccgaagc 43980

cgagttcacc gagatcgccg acgtgaaggt gcgcggcacc gtcaacctgg acgagctctg 44040

cccggacctc gacgcgttcg tgttgttctc ctccaacgcg ggcgtgtggg gcagtccggg 44100

gctcgcctcc tacgcggcgg ccaacgcctt cctcgacggc ttcgcgcggc ggcgccggag 44160

cgagggcgcg ccggtgacgt ccatcgcctg ggggctctgg gccgggcaga acatggccgg 44220

ggacgagggc ggcgagtacc tgcgcagcca gggcctgcgg gccatggacc cggatcgggc 44280

cgtcgaggaa ctgcacatca ccctcgacca cggtcagacg tccgtgtcgg tcgtggacat 44340

ggatcgcagg cggttcgtcg agctgttcac cgcggcccgg caccggccgc tgttcgacga 44400

gatcgccggt gcccgggcgg aagcccggca gagcgaggag ggcccggcgc tcgcccagcg 44460

gctcgcggcg ctgtcgacgg ccgagaggcg cgagcacctc gcccacctga tccgcgccga 44520

ggtcgccgcg gtgctcggcc acggcgacga cgcggcgatc gaccgcgacc gcgccttccg 44580

cgacctcggc ttcgactcca tgaccgccgt cgacctgcgg aaccggctcg ccgcggtgac 44640

cggggtgcgg gaagccgcga cggtggtctt cgaccacccg accatcaccc ggctcgccga 44700

ccactacctg gagcggctcg tcggcgcagc agaggcggag caagccccgg cgctcgtgcg 44760

cgaggtgccg aaggatgccg acgacccgat cgcgatcgtc ggcatggcct gccgcttccc 44820

cggcggcgtg cacaaccccg gtgagctgtg ggagttcatc gtcggcggcg gagacgccgt 44880

gacggagatg cccaccgacc gcggctggga cctcgacgcg ctgttcgacc ccgacccgca 44940

gcgccacgga accagctact cgcgacacgg cgcgttcctc gacggggccg ccgacttcga 45000

cgcggcgttc ttcgggatct cgccgcgcga ggcgctggcg atggacccgc agcagcgcca 45060

ggtcctggaa acgacgtggg agctgttcga gaacgccggc atcgacccgc actcgctgcg 45120

gggcagcgac accggcgtct tcctcggcgc cgcgtaccag ggctacggcc aggacgcggt 45180

ggtgcccgag gacagcgagg gctacctgct caccggcaac tcctccgccg tggtgtccgg 45240

ccgggtcgcc tacgtgctgg ggctggaagg ccccgcggtc acggtggaca cggcgtgttc 45300

gtcgtcgttg gtggccttgc attcggcgtg tgggtcgttg cgtgacggtg actgcggtct 45360

tgcggtggcc ggtggtgtgt cggtgatggc gggcccggag gtgttcaccg agttctcccg 45420

ccagggcggc ttggccgtgg acgggcgctg caaggcgttc tccgcggagg ccgacggctt 45480

cggtttcgcc gagggcgtcg cggtggtcct gctccagcgg ttgtccgacg cccgcagggc 45540

gggtcgccag gtgctcggcg tggtcgcggg ctcggcgatc aaccaggacg gcgcgagcaa 45600

cggtctcgcg gcgccgagcg gcgtcgccca gcagcgcgtg atccgcaagg cgtgggcgcg 45660

tgcggggatc acgggcgcgg atgtggccgt ggtggaggcg catgggaccg gtacgcggct 45720

gggcgatccg gtggaggcgt cggcgttgct ggctacttac ggcaagtcgc gcgggtcgtc 45780

gggcccggtg ctgctgggtt cggtgaagtc gaacatcggt cacgcgcagg cggccgcggg 45840

tgtcgcgggc gtgatcaagg tggtcctggg gttgaaccgc ggcctggtgc cgccgatgct 45900

ctgccgcggc gagcggtcgc cgctgatcga atggtcctcg ggtggtgtgg aacttgccga 45960

ggccgtgagc ccgtggcctc cggccgcgga cggggtgcgc cgggccggtg tgtcggcgtt 46020

cggggtgagc gggacgaacg cgcacgtgat catcgccgag cccccggagc ccgagccgct 46080

gccggaaccc ggaccggtgg gcgtgctggc cgctgcgaac tcggtgcccg tactgctgtc 46140

ggccaggacc gagaccgcgt tggcagcgca ggcgcggctc ctggagtccg cagtggacga 46200

ctcggttccg ttgacggcat tggcttccgc gctggccacc ggacgcgccc acctgccgcg 46260

tcgtgcggcg ttgctggcag gcgaccacga acagctccgc gggcagttgc gagcggtcgc 46320

cgagggcgtt gcggctcccg gtgccaccac cggaaccgcc tccgccggcg gcgtggtttt 46380

cgtcttccca ggtcagggtg ctcagtggga gggcatggcc cggggcttgc tctcggtccc 46440

cgtcttcgcc gagtcgatcg ccgagtgcga tgcggtgttg tcggaggtgg ccgggttctc 46500

ggcctccgaa gtgctggagc agcgtccgga cgcgccgtcg ctggagcggg tcgacgtcgt 46560

acagccggtg ttgttctccg tgatggtgtc gctggcgcgg ctgtggggcg cttgcggagt 46620

cagcccctcg gccgtcatcg gccattcgca gggcgagatc gccgccgcgg tggtggccgg 46680

ggtgttgtcg ctggaggacg gcgtgcgcgt cgtggccctg cgcgcgaagg cgttgcgtgc 46740

gctggcgggc aagggcggca tggtctcgtt ggcggctccc ggtgaacgcg cccgcgcgct 46800

gatcgcaccg tgggaggacc ggatctccgt cgcggcggtc aactccccgt cctcggtcgt 46860

ggtctccggc gatccggagg cgctggccga actcgtcgca cgttgcgagg acgagggcgt 46920

gcgcgccaag acgctcccgg tggactacgc ctcgcactcc cgccacgtcg aggagatccg 46980

cgagacgatc ctcgccgacc tcgacggcat ctccgcgcgg cgtgccgcca tcccgctcta 47040

ctccacgctg cacggcgaac ggcgcgacgg cgccgacatg ggtccgcggt actggtacga 47100

caacctgcgc tcccaggtgc gcttcgacga ggcggtctcg gccgccgtcg ccgacggtca 47160

cgccaccttc gtcgagatga gcccgcaccc ggtgctcacc gcggcggtgc aggagatcgc 47220

cgcggacgcc gtggccatcg ggtcgctgca ccgcgacacc gcggaggagc acctgatcgc 47280

cgagctcgcc cgggcgcacg tgcacggcgt ggccgtggac tggcggaacg tcttcccggc 47340

ggcacctccg gtggcgctgc ccaactaccc gttcgagccc cagcggtact ggctcgcgcc 47400

ggaggtgtcc gaccagctcg ccgacagccg ctaccgcgtc gactggcgac cgctggccac 47460

cacgccggtg gacctggaag gcggcttcct ggtccacggg tccgcaccgg agtcgctgac 47520

cagcgcagtc gagaaggccg gaggccgcgt cgtgccggtc gcctcggccg accgcgaagc 47580

gctcgcggcg gccctgcggg aggtgccggg cgaggtcgcc ggcgtgctct cggtccacac 47640

cggcgccgca acgcacctcg ccctgcacca gtcgctgggt gaggccggcg tgcgggcccc 47700

gctctggctg gtcaccagcc gagcggtcgc gctcggggag tccgagccgg tcgatcccga 47760

gcaggcgatg gtgtggggtc tcgggcgcgt catgggcctg gagaccccgg aacggtgggg 47820

cggtctggtg gacctgcccg ccgaacccgc gccgggggac ggcgaggcgt tcgtcgcctg 47880

cctcggcgcg gacggccacg aggaccaggt cgcgatccgt gaccacgccc gctacggccg 47940

ccgcctcgtc cgcgccccgc tgggcacccg cgagtcgagc tgggagccgg cgggcacggc 48000

gctggtcacc ggcggcaccg gtgcgctcgg cggccacgtc gcccgccacc tcgccaggtg 48060

cggggtggag gacctggtgc tggtcagcag gcgcggcgtc gacgctcccg gcgcggccga 48120

gctggaagcc gaactggtcg ccctcggcgc gaagacgacc atcaccgcct gcgacgtggc 48180

cgaccgcgag cagctctcca agctgctgga agaactgcgc gggcagggac gtccggtgcg 48240

gaccgtcgtg cacaccgccg gggtgcccga atcgaggccg ctgcacgaga tcggcgagct 48300

ggagtcggtc tgcgcggcga aggtgaccgg ggcccggctg ctcgacgagc tgtgcccgga 48360

cgccgagacc ttcgtcctgt tctcgtccgg agcgggggtg tggggcagtg cgaacctcgg 48420

cgcctactcc gcggccaacg cctacctcga cgcgctggcc caccgccgcc gtgcggaagg 48480

ccgtgcggcg acgtccgtcg cgtggggcgc ctgggcgggc gagggcatgg ccaccggcga 48540

cctcgagggg ctcacccggc gcggcctgcg cccgatggcg cccgagcgcg cgatccgcgc 48600

gctgcaccag gcgctggaca acggcgacac gtgcgtttcg atcgccgacg tcgactggga 48660

gcgcttcgcg gtcggcttca ccgccgcccg gccgcgtccg ctgctggacg agctcgtcac 48720

gccggcggtg ggggccgtcc ccgcggtgca ggcggccccg gcgcgggaga tgacgtcgca 48780

ggagttgctg gagttcacgc actcgcacgt cgcggcgatc ctcgggcatt ccagcccgga 48840

cgcggtcggg caggaccagc cgttcaccga gctcggcttc gactcgctga ccgcggtcgg 48900

gctgcgcaac cagctccagc aggccaccgg gctcgcgctg cccgcgaccc tggtgttcga 48960

gcaccccacg gtccgcaggt tggccgacca cataggacag cagctcgaca gcgggactcc 49020

cgcccgggaa gcgagcagcg ctcttcgcga cggctaccgg caggcgggcg tgtcgggcag 49080

ggtccggtcc tacctcgacc tgctggcggg gctgtcggac ttccgcgagc acttcgacgg 49140

ctccgacggg ttctccctcg atctcgtgga catggccgac ggtcccggag aggtcacggt 49200

gatctgctgc gcgggaacgg cggcgatctc cggtccgcac gagttcaccc ggctcgccgg 49260

ggcgctgcgc ggaatcgctc cggttcgggc cgtgccccag cccggctacg aggagggcga 49320

acctctgccg tcgtcgatgg cggcggtggc ggcggtgcag gccgatgcgg tcatcaggac 49380

acagggggac aagccgttcg tggtggccgg tcactccgcg ggggcactga tggcctacgc 49440

gctggcgacc gaactgctcg atcgcgggca cccgccacgc ggtgtcgtcc tgatcgacgt 49500

ctacccgccc ggtcaccagg acgcgatgaa cgcctggctg gaggagctga ccgccacgct 49560

gttcgaccgc gagacggtgc ggatggacga caccaggctc accgccctgg gcgcctacga 49620

ccgcctcacc ggtcagtggc gaccccggga aaccgggctg ccgacgctgc tggtcagcgc 49680

cggcgagccg atgggtccgt ggcccgacga cagctggaag ccgacgtggc ccttcgagca 49740

cgacaccgtc gccgtccccg gcgaccactt cacgatggtg caggaacacg ccgacgcgat 49800

cgcgcggcac atcgacgcct ggctgggcgg agggaattca tgaccacgac cgatcgcgcc 49860

gggctgggca ggcagctcca gatgatccgc ggcctgcact ggggttacgg cagcaacggc 49920

gacccttacc cgatgctgct gtgcggacac gacgacgacc cgcagcgccg gtaccgctcg 49980

atgcgcgagt ccggtgtgcg gcgcagccgc accgagacgt gggtggtggc cgaccacgcc 50040

accgcccggc aggtgctcga cgaccccgcg ttcacccgcg ccaccggacg cacaccggaa 50100

tggatgcggg ccgcgggcgc gccacccgcc gagtgggccc agccgttccg ggacgtgcac 50160

gccgcgtcct gggaaggcga ggtccccgac gtcggggaac tggcggagag cttcgccggt 50220

ctgctccccg gcgcgggcgc gcggctggac ctggtcggcg acttcgcctg gcaggtaccg 50280

gtgcagggca tgaccgccgt gctcggcgca gccggagtgc tgcgcggcgc cgcgtgggac 50340

gcccgcgtca gcctggacgc ccagctcagc ccgcagcagc tcgcggtgac cgaagcagcg 50400

gtcgcggcac tgcccgccga ccccgcactg cgcgccctgt tcgccggggc cgagatgacc 50460

gcgaacaccg tggtcgacgc ggtcctggcc gtctcggccg aaccggggct ggccgaacgg 50520

atcgccgacg accccgccgc cgcgcagcga accgtcgccg aggtgctgcg cctgcacccg 50580

gcattgcacc tggagcggcg cacggccacc gcagaggtgc ggctcggcga gcacgtgatc 50640

ggcgaaggcg aggaggtcgt ggtcgtcgtc gcggcggcca accgcgaccc ggaggtcttc 50700

gccgagcccg accgcctcga cgtggaccgc cccgacgccg accgcgcgct gtcggcacat 50760

cgcggccacc ccggcaggct ggaggagctg gtcaccgcgc tcgccaccgc cgcactgcgg 50820

gccgcggcca aggcgctgcc cggactcacg cccagcggcc cggtcgtccg gcgccgccga 50880

tcacccgtcc tgcggggaac caaccgctgc cccgtcgagc tctgaggtaa ccgcgatgcg 50940

cgtcgtcttc tcctccatgg ccagcaagag ccacctcttc ggcctcgtcc ccctcgcatg 51000

ggcgttccgc gcggcggggc acgaggtccg cgtggtcgcg tccccggcgc tcaccgagga 51060

catcaccgcg gccgggctga ccgccgtccc ggtcggcacc gacgtcgacc tcgtggactt 51120

catgacccac gcgggccacg acatcatcga ctacgtccgg agcctggact tcagcgagcg 51180

ggaccccgcc accttgacct gggagcacct gctgggcatg cagaccgtgc tcaccccgac 51240

cttctacgcc ctgatgagcc cggacacgct catcgaaggc atggtctcgt tctgccggaa 51300

gtggcggccc gacctggtca tctgggagcc gctcaccttc gccgcgccca tcgcggcggc 51360

ggtgaccgga acgccgcacg cgcggctgct gtggggaccc gacatcacca cccgggcgcg 51420

gcagaacttc ctcggcctgc tgcccgacca gccggaggag caccgggagg acccgctcgc 51480

cgagtggctc acctggacgc tggagaagta cggcggcccg gccttcgacg aggaggtggt 51540

cgtcgggcag tggacgatcg accccgcccc ggccgcgatc aggctcgaca ccggcctgaa 51600

gaccgtcggg atgcgctacg tcgactacaa cgggccgtcc gtggtgccgg aatggctgca 51660

cgacgagccc gagcgccgcc gcgtgtgcct cacgctcggg atctccagcc gcgagaacag 51720

catcgggcag gtctccatcg aggagctgct gggtgccgtc ggcgacgtcg acgccgagat 51780

catcgcgacc ttcgacgcgc agcagctaga aggcgtcgcg aacatcccgg acaacgtccg 51840

cacggtcggc ttcgtcccga tgcacgcgct gctgccgacc tgcgcggcga cggtgcacca 51900

cggcggaccc gggagctggc acaccgcggc gatccacggc gtgccgcagg tgatcctgcc 51960

cgacggctgg gacaccggcg tgcgcgcgca gcgcacgcag gaattcgggg cggggatcgc 52020

gctgcccgtg cccgagctga cccccgacca gctccgggag tcggtgaagc gggtcctcga 52080

cgacccggcc caccgcgccg gcgcggcgcg gatgcgcgac gacatgctcg cggagccgtc 52140

accggccgag gtcgtcggca tctgcgagga actggccgca ggaaggagag aaccacgatg 52200

accaccgacg ccgcgacgca cgtgcggctc gggcgttccg cgctgctcac cagcaggctc 52260

tggctcggca cggtgaactt cagcggacgc gtcgaggacg acgacgcgct gcgcctgatg 52320

gaccacgccc gggaccgcgg catcaactgc ctcgacaccg ccgacatgta cggctggcgg 52380

ctctacaagg gccacaccga ggagctggtg ggcaggtggc tggcccaggg cggcggacgg 52440

cgcgaggaca ccgtgctggc gaccaaggtc ggcggcgaga tgagcgagcg cgtcaacgac 52500

agcgggctgt cggcgcggca catcatcgcc tcctgcgagg gatcgctgcg caggctgggc 52560

gtcgaccaca tcgacgtcta ccagatgcac cacatcgacc ggtccgcgcc gtgggacgag 52620

gtgtggcagg ccatggacag cctcgtcgcc agcggcaagg tctcctacgt cggctcgtcg 52680

aacttcgcgg gctggcacat cgccgccgcg caggagaacg ccgcccgccg ccactccctg 52740

ggcatggtct cccaccagtg cctgtacaac ctggcggtcc ggcacgccga gctggaggtg 52800

ctgcccgccg cgcaggccta cgggctcggc gtcttcgcct ggtcgccgct gcacggcggc 52860

ctgctcagcg gagcgctgga gaagctggcc gcgggcaccg cggtgaagtc ggcgcagggc 52920

cgtgcgcagg tgctgttgcc gtccctgcgc ccggcgatcg aggcctacga gaagttctgc 52980

cgcaacctcg gcgaagaccc ggccgaggtg gggctcgcat gggtgctgtc ccggcccggc 53040

atcgccggcg ccgtcatcgg cccgcgaacc cccgagcagc tcgactccgc gctgaaggcg 53100

tccgcgatga ccctggacga gcaggcgctg tccgaactgg acgagatctt ccccgcggtg 53160

gcctccggcg gcgcggcgcc ggaagcctgg ttgcagtgag cacaagagga accgagaaag 53220

gatacggctg gtgagcgtga agcagaagtc agcgttgcag gacctggtcg acttcgccaa 53280

gtggcacgtg tggaccaggg tgcggccgtc cagccgtgcg cgcctggcct acgagctgtt 53340

cgccgacgac cacgaggcca cgaccgaggg cgcctacatc aacctcggct actggaagcc 53400

cgggtgcgcc ggcctggagg aggccaacca ggagctggcg aaccagctcg ccgaggccgc 53460

ggggatcagc gagggcgacg aggtgctcga cgtcgggttc gggctcggcg cgcaggactt 53520

cttctggctc gagacgcgca agccggccag gatcgtcggc gtcgacctga ccccgagcca 53580

cgtccgcatc gcctccgagc gcgcggagcg cgagaacgtg caggaccgcc tgcagttcaa 53640

ggagggctcg gcgaccgacc tgcccttcgg cgcggagacc ttcgaccgcg tcacctccct 53700

ggagtcggcc ctgcactacg agccgcggac cgacttcttc aagggcgcgt tcgaggtgct 53760

caagcccgga ggcgtgctgg ccatcggcga catcatcccg ctcgacctgc gcgagcccgg 53820

ttccgacgga ccgccgaagc tggcgccgca gcggtcggga tcgctgtccg gcggcatccc 53880

ggtggagaac tgggtgcccc gcgagaccta cgccaagcag ctccgcgaag cgggcttcgt 53940

cgacgtcgag gtgaagtcgg tccgcgacaa cgtgatggag ccctggctgg actactggct 54000

gcgcaagctg caggacgagt cgttcaagaa gagcgtgagc aggctcttct acagccaggt 54060

caagcggtcg ctgaccagcg actcgggcat gaagggcgag ctgcccgcgc tcgacttcgt 54120

gatcgcctca gcccgcaagc ccggcgcgta gcaggtcccg gcgcacggca ccggccacct 54180

cggtgacctg ttcacccagg tagaagtgcc cgccggggaa ggtcctggtc cggccgggga 54240

tgaccgagtg ctgcaaccag cgctcggcgt ccccggtcgc ggtcagcggg tcggcgtcgc 54300

cgcacagcgc ggtgatgccg gcccgcagcg gtggtccgtc cgcccaggcg taggagcgca 54360

gcacgcggta gtcggcgcgc agcaccggca gcgacatggc gagcagggcc tcgtcggcca 54420

gcgccgcctc gctggtgccg agccggcgca gctcgtccac cagaccgtcg tcgccgggca 54480

ggtcggtgcg gcgctcgtgc acgcgcgggg cggtctgccc ggacacgaac agccgcagcg 54540

gcccgccgcc gggctcgcgt tcgagcctgc gcgccgtctc gtaggcgatc aacgcgccca 54600

tgctgtgccc gaacagggcg aacgggccgt cgccgcccga cgcgcgcagc acggcggcca 54660

cctcgtcggc gatctcgccg gcggtgccca ggggctcctc gtcgcggcgg tcctggcgcc 54720

ccgggtactg cacggcgtgc acgtcgatct cgggcgccaa ggcgcgcgcg aggtcgaggt 54780

aggagtcggc cgcggctccc gcgtgcggga agcacaccag ccgcgcccgg tgctcgacgg 54840

gaggaccgaa ccgccgcagc caggtgctca tccgtcgagc cgcaccggta ggtggtcgat 54900

gccccgcagc agcagcgaac gccgccacac cacgtcgtcg gcgtcgattc ccagcgacag 54960

agcggggaag cggccgaaca gcgcccgcag cgccacctcg ccctccagct tggccagcgg 55020

ccggcccatg cagaagtgga tgccctgccc gaacgacagg tggccgcggg tgtcgcgggt 55080

gacgtcgaag cggtgggggt ccgggaactg cttcgggtcg cggttggccg cgccgttcgc 55140

gaccagcacc gtgctgtact gggggatcgc gacaccgccg atctccacct cctccgcggc 55200

gaagcgcgtg gtggtctccg gcggagcgat gtagcgcagg atctcctcga cggcgttggg 55260

cagcgccgac gggtcccgcc gcaccagcgc gagctggtcc gggtgggtga gcagcaggta 55320

ggtgccgatc ccgatgaggc tcaccgacgc ctcgaaaccg gccagcagca gcaccagcgc 55380

gatggaggtc agctcgtcgg cgctgagccg accgtcatcg tcgtcctgga ccctgatcag 55440

cgcggacagc aggtcgtcgc cgggctcggt gcggcggcgc tcgaccaggt cgaggatgaa 55500

gttgacgacc tccctggccg cctgcccgcg ctgttcggcc cgctccgggt ccatgaccag 55560

gatctccgag ctccaccgcc cgaactcccc gcggtacttc tcgtcgacgc cgagcagctc 55620

gcagatgacc ttgatgggca gcgggtgggc gaagcggtcg acgatgtcga ccacgccgga 55680

gtcgcccacc tcgtcgagca gctccgcggt gatctgctcg acgcggggcc gcatcgcctc 55740

cacgcggcgg acggtgaact cctgcgacac cagcttgcgc agccgggtgt gggtcggcgg 55800

gtcgctggtg cccatgttgg tggcgaagta gttccgcacg tcctcgggga aaccgaggta 55860

tgccgggaac tcgacctcca cgcccgggta cttcttcttc gggtcgctgc tcaggcgcag 55920

gtcgctcagc gcggccttcg cctcgtcgta gccggtgacc agccacgcgt cctggccgag 55980

gaagcgcacc ggcgtcaccg gcgcggtctc gcgcagctcg gcgtaggtgc ggtaccagtc 56040

gacgtggaag gagtcgcttt cgagatcggg aacggtcgtc atacgacttc cagtcggggt 56100

agggggaaga tcagctttcc gccgccagcg aggaactcct gctcgcgttc gacgaacccg 56160

tcgcggtaga tccagggcag caccagcagc tggtcggggc ggcgcgactt ggcctcttcc 56220

tccgacacga tcgggatccc ggtgcccggg gtgtagcaac cggacttctc cgggctgacc 56280

tcgccgatgc agggcaggtc ctgctcggtg agcccgcagt actggaggat gacgttgccc 56340

ttggtcgagg cgccgtagcc gagcgtcagc ttgccctcgg cgcgggactt ggccaggaac 56400

tccagcagtt ggtcgcgctg gcgctcggtt tcgcgcgcga acgcctcgta gggggccatg 56460

gtgtcgagct tcgcggcggc ctcgcgggcg cggatgcgct ccaggcccgc ctcgtcgacc 56520

gggtggccgc tgccttgctt ggccagcacg gcgcacaggc tgccgccgta gacgtcggtg 56580

atctcggcgt cgacgacctt cagcccgacc cgctgcgcca tccactcgat ctgcgcgagc 56640

gcgtagtact cgaggtgctc gtggcacacg acgtcgtagg cgccggcgtc gagcatggag 56700

ggcaggtagc tctgctccat cagccagagc ccgtcgtcgg ccaggatgtc gtgcacgtcc 56760

cgcatgaacc gcatcggctc gggcaggtcg tagaacatcg cgatggaggt gacgaccttt 56820

gcgcggcgct cgccgtagcg ctcctcgaag gcgtcggccg agaagaaccc ggtgaccagg 56880

tcggcctccg gcgggtagag gtcgcggaac ttctcgccca ccaggtcgaa gccggccagc 56940

ttcggggcgt cgggcaggta tccgcgcagc agcgtggagt cgttgctgcc gatgtcgacc 57000

accaggtcgt ccgggcccac cggcaccatg ccccgcagct tggccacctt gccgtgcagg 57060

tggttgatca tgaacgggcg gatgccggac cggtagccgt atccctcgtt gtacatcagc 57120

ccgaaatcgg cgctctcgcg cagctggacc agtccgcagc cgggcggcgc gcacttgacc 57180

agttcgagcg ggaccgaggg cacgatttcg tcgcggttcc gggggaacac tccggtgagg 57240

gcctgatctc ccaggtgcag cacggactcg aggtcatggt tcccgcatat gcggcatttg 57300

cctagtccca caaggaagat cataacgagc gcggcgccgc ggccaacccg tcgagctttg 57360

tgtcctggat cacatcagcc ggtttacgac tcccgttgcg tccattgtgg atcgcgcttt 57420

ccgctgcgat gtccggccga ggagcggcgt ccgaaaagtc cgtgtcggca ccgcgcgccg 57480

gtgttaggtt gatcaagctg aaaattgttt gcatgactgg gggtgagcgc gtgaaacggc 57540

tggtcatccg catcgcgccg ctgttgttgg tagttccgct gctcgtggcg gccgtatcgc 57600

cggtacgcca ttcgcagcgg gtggatgagc tgatcggaca gctgaccctg gacgagaagc 57660

tctccttcgt ctactgggac tacaacgaga aggacccgct ggcgaagctc tggctgcccg 57720

gagtaccgcg cctgggcatc ccgcagatcc gcggcaccga cgggcccgcc ggggtcacca 57780

tccaccagcc ggcgatcgcc atgcccgcgc cggtcgcgct ggcgtcggcg ttcgacgacc 57840

ggctggccca cgagtacggc acggtgctcg gcagggaggg ccgcgcgttc gagcaggaca 57900

tcatcctggg cccgatggtc aacaacatcc gcgtcccgca ggcgggacgc aacttcgaga 57960

ccttcagcga ggacccgctg gtcaccgccc gcaccgcggc cgcccagatc cgcggcatcc 58020

acagccaggg gctgatgacc agcgccaagc actacgcggc caacacccag gagaccgacc 58080

ggttcaccat cgacgtcgac gtggaccagc gcaccctgcg ggagctggag ctgcccggct 58140

tcgaggccgc ggtcgccgcc ggcgcgacat cggtgatgtg cgcctacccc aaggtcaacg 58200

gcacgcacgc ctgcggccac cggcagctgc tcaccgagat cctcaaggag cagtggggtt 58260

tcaagggctg ggtgatgtcg gactggaccg ccacccacgc caccgaggac ctcgtcgccg 58320

ggctcgacca ggagatgggc gtcgaggtcc gcgaggacgg cagcctgttc aggggcaagt 58380

acctgggcga ggcgctgaag aaggccatcc gggagggccg cattcccgag tccgcgctcg 58440

acgcgtcggt gcgccgcatc ctcacccagt tcgagcggtt cggcctgctc gacgagacca 58500

agccgccgcg gccggagcgc gacgtcgcgg gcggcacccg catcgcccag gaggtcgccg 58560

agtcgggcgc ggtgctgctg cgcaacgagg gcggcgtgct gccgctggat cccgcggcgg 58620

gccaggacat cgcggtcatc gggccgtcgg cccaacagcc gaaggtcacc gggctgggca 58680

gctcctacgt cgagcccgac ttcgccaacg cgccgctgga caccatcacc cagcgcgtcg 58740

gcagcggcgg tcgcgtcggg tactccgtcg gcgaggagct gaagggcgct ccgatcccgg 58800

aaaccgcgct gcaaccggcg ttcgtgcccg gcgaggtcac gccgccccca tcaggcggcg 58860

tgatctacga cggcaggctg aaggtggacg ccgacgggct ctaccgcatc gcggccagga 58920

tcgacggagg caacgggagc ctgcagatcg acggcggcgc cccgatcggc gtcggcgacg 58980

tcttcggccc gctcaccagc gtgccggtct ggctcaccaa gggcgagcac accatccaga 59040

tgaccggagc ggcgccggtg ggcggcggtt cgctcgacgt cgacctgacc tgggtcaccc 59100

cggggcacgc ccagcgcgag ttcgacgcgg cggtggagcg ggcgcgcgac tccgacgtcg 59160

cggtggtttt cgcctacgac gacggcgcgg agaccgccga ccgcacgtcg ctgagcctgc 59220

ccggcaccca ggacaagctg atcgacgccg tcgcctcggt caacccgaac accgtcgtgg 59280

tgctcaacac cggctcgtcg gtcacgatgc cgtggctgga caagacccgc gcggtgctcg 59340

acatgtggta cccgggacag gccggggcgg aggccaccac ggcgctgctg ttcggcgacg 59400

ccgagcccgg cggccggctc acccagacct tcccggtgtc gcaggaacgc acgcccgtcg 59460

gcggcgaccc ggcgcgcttc cccggcgtgg acgggaaggt gcactactcg gagggcatct 59520

tctcgggcta ccgctggtac gaccgggagg gtgtggaccc gcttttcccg ttcgggcacg 59580

ggctttccta caccacgttc gagcgcaccg acccggtcgt cgagcgcacc cgcgacggcc 59640

tcgacgtgac ggtgaccgtg cgcaacaccg gtcagcggcg cggcagcgac gtcgtgcagg 59700

tctacctcgg ccccagcccg caggttccgc tggaccaggc accgcggcag ctcgccgggt 59760

accagaaggt cgaactcgca ccgggcgaga ccaagcgcgt ccgggtgcac gtcgcggagc 59820

gggccctcca gcactgggac gaagccgcgg gcggctggaa gctcgggggc ggcaagcgag 59880

cggtggagat cggctcgtcg tcgcgcgaca tcgacctccg ggccgacatc aacctctgat 59940

tcctctcgac caacaggccg ctcgacggcc tgccccgttc cgcgtgccgg ggactaccgc 60000

tgcccgggtc cgccgcgtcg ccctggacgt ccgccggtcc gtccaccccc ggacggcccc 60060

cgccgcccgc cttggtcccc ctgctcgcgc tcgccggttc gtccgtcgtc gcgattgcgt 60120

ccgctgctgc gacgatcgcc gtggccgctg gcttgccggt ccctgtggtc gcggccgccg 60180

gttcgccgat cgccgcgatc ctgcccgccg gttcgccggt ccccgtggtc ccgtccgccg 60240

gttcgcctgc cccgctgggg ctgcccaccc ctcggttcgt tcttctgatc cagccgctcg 60300

aacagccgga gccactgctc cggccggacg taggcgacca cggcgccgcc cccgaccccc 60360

gcgtggtcga gcgccgcctc gacgcggcgc ctcgggtact tgcgcagaag cgacgcctgg 60420

atgttgccgc cgacgccggt gaagcacagc tcgaccatcg actcgtagcg ttcgagcgcc 60480

gcgccttcca gcagcggttc ggcgcgcctg cgcagccgca tgatcgccga gtcgaccttg 60540

ggcaccggct tgaacagccg gcggtcgacc ttctcgacga actcccactc gaacagcggc 60600

caggtcatca ccgtgaggcg gctccagcgg ccgtaatcgc cggtccgctt ccgggcgaac 60660

tccagctgcg tgaccatcgt cgccgtctcg atcgtcggcg cctccaggca ccagtccacg 60720

atcgccgagg tgatgccgta ggggatcgcg ccgacgaagg cgaacggctc gggcggcggt 60780

tcggcggtga ggaagtcggc gttgacgact tcgatgttcg ggtggccgga aagcttctcc 60840

cgcaacgact tcgccagccg ggggtcgatc tcgtacgacg tcacctgacg cgcgcggtcg 60900

gcgagttccc tggtgagcag cccttcgccg gggccggctt ccagcaccgg cagatcgggc 60960

cgcagctcgg ctgtctcggc gatgcgcgcg atggtcttgc ggtcgcggag gaagttctgc 61020

ccgaactggc gccggttgcg gtcccgctcg gtacggccca gcaccggccg gttctggttg 61080

gggtgctgcc gatcctggtt gcggcgacgc gggcgcggct gctcgtccga actgctcacc 61140

gctggatcct accaaccggc acgattgtcc agcccacaac agcatcgcgg tgccacgtgt 61200

ggaccgcgtc ggtcagatcc tccccgcacc tctcgccagc cgtcaagatc gaccgcgtgc 61260

acctgcgatc gccgatcaac cgcgactagc atcgggcgca agccgccact cgaacggaca 61320

ctcgcatgga cgtccccttc ctggacctgc aagccgcgta cctcgaactc cggtccgaca 61380

tcgaccaggc gtgccggcgc gtgctcgggt cgggctggta cctgcacggg ccggagaacg 61440

aggcgttcga ggcggagttc gccgcctact gcgagaacgc gcactgcgtg acggtcggca 61500

gcggatgcga cgcgctggag ctgagcctgc tggcgctggg cgtcggtcag ggcgacgagg 61560

tgatcgtgcc gtcccacacc ttcatcgcga cgtggctcgc ggtgtcccgc gtgggcgcgg 61620

tgccggtgcc agtcgaaccg gaaggcgtga gccacacgct cgatccggcc ctggtggaac 61680

aggccatcac gccgcggacg gccgccatcc tgccggtgca cctctacggc caccctgcgg 61740

acctcgacgc gctgcgagcg atcgccgacc gccacggcct ggcactggtg gaggacgtcg 61800

cacaggcggt cggcgcccgc caccgcggcc accgcgtcgg cgccggctcg aacgccgcgg 61860

ccttcagctt ctacccgggc aagaacctcg gagcgctggg cgacggcggc gcggtcgtca 61920

ccaccgaccc ggccctggcc gagcgcatcc gcctgctgcg caactacggc tcgaagcaga 61980

agtacgtgca cgaggtccgc ggcaccaacg cccgcctcga cgaactccag gcggcggtcc 62040

tgcgcgtcaa gctccgccac ctcgacgact ggaacgcccg ccgcacaacg ctggcgcagc 62100

actaccagac ggaactgaag gacgtccccg gcatcacgct cccggaaacc cacccctggg 62160

ccgactcagc ctggcacctc ttcgtcctcc gctgcgagaa ccgcgaccac ctccaacgcc 62220

acctgaccga cgcgggcgtc cagaccctca tccactaccc gaccccggtc cacctctcac 62280

ccgcctacgc cgacctcggc ctgcccccag gctccttccc cgttgcggag tctctggcgg 62340

gggaggtcct cagcctcccc atcggccccc acctctccag ggaggcggca gaccacgtca 62400

tcgcgaccct caaggctggg gcttgatctg cgttcggctc acgcgcagtg gaagcacacc 62460

cgccgcgcaa tccgcgcccg gtccgctccc gcgttcaccg ggatcgcttc cccggtcttg 62520

atccgctccc aagcgttggg ttcgtcgtgg atttcgcccc actcatcgaa gctctccccg 62580

tagtggttgc acttcccggg caggtgggcc gtgtccgctc gatacggtcc cgcggagatc 62640

gacacgtggg gagggttcga tgacgtcgcc gggcggagac gacggcggtg gcaggcaccc 62700

ggtcgcggag accggtggtg aaccggtcgc ggaggtgttc atcccggctc aagcgggtgc 62760

ggacggcagg caggcccagt tggagctgcg ccagacgacc gggggcgcgc tcgcgctggt 62820

gctctacacg agcctggaac gactcgtgga gtcaattcgg cgaccctacg cccccaactg 62880

agagaactca aaggttaccc cagttggggc actactcccg aaaaccgctt ctgacctggg 62940

aaaacgtgaa gccccggggc atccgctgag ggttgccgcc ggggcttcgg tgtgtccgtc 63000

agtacgggcc atagaggggc gtcgtgtaac ccacccaggg cgattgctcc ggcacgggga 63060

agccgcgcca cgccttcggg acgtctggaa tcgctagagc ttgcatgcct gcaggtcgac 63120

ggatcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc 63180

catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt 63240

gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt 63300

ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag 63360

gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc 63420

caaccaggaa gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga 63480

ttgaggaaaa ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc 63540

agggctacaa aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca 63600

atggcgacct gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca 63660

cggcgcggtt cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg 63720

acgagcttgg caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt 63780

agccgctaaa acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc 63840

aagaagagcg acttcgcgga gctggtgaag tacatcaccg acgagcaagg caagaccgat 63900

ccccggggac ctgcaggtcg actctagcta gctggccagg gcccgatacg tcgcggtgag 63960

ttcaggcttt ttcatatctc attgcccccg gacgagcgtc tgctccgcca ttcgccgtcc 64020

gccgtgccaa tcggatcagc cgtccaaatg cgggattttc gttagtcgga ggccaaacgg 64080

cattgagcgt cagcatatca tcagcgagct gaagaaagac aatccccgat ccgctccacg 64140

tgttgcccca gcaatcagcg cgaccttgcc cctccaacgt catctcgttc tccgctcatg 64200

agctcagcca atcgactggc gagcggcatc gcattcttcg catcccgcct ctggcggatg 64260

caggaagatc aacggatctc ggcccagttg acccagggct gtcgccacaa tgtcgcggga 64320

gcggatcaac cgagcaaagg catgaccgac tggaccttcc ttctgaaggc tcttctcctt 64380

gagccacctg tccgccaagg caaagcgctc acagcagtgg tcattctcga gataatcgac 64440

gcgtaccaac ttgccatcct gaagaatggt gcagtgtctc ggcaccccat agggaacctt 64500

tgccatcaac tcggcaagat gcagcgtcgt gttggcatcg tgtcccacgc cgaggagaag 64560

tacctgccca tcgagttcat ggacacgggc gaccgggctt gcaggcgagt gaggtggcag 64620

gggcaatgga tcagagatga tctgctctgc ctgtggcccc gctgccgcaa aggcaaatgg 64680

atgggcgctg cgctttacat ttggcaggcg ccagaatgtg tcagagacaa ctccaaggtc 64740

cggtgtaacg ggcgacgtgg caggatcgaa cggctcgtcg tccagacctg accacgaggg 64800

catgacgagc gtccctcccg gacccagcgc agcacgcagg gcctcgatca gtccaagtgg 64860

cccatcttcg aggggccgga cgctacggaa ggagctgtgg accagcagca caccgccggg 64920

ggtaacccca aggttgagaa gctgaccgat gagctcggct tttcgccatt cgtattgcac 64980

gacattgcac tccaccgctg atgacatcag tcgatcatag cacgatcaac ggcactgttg 65040

caaatagtcg gtggtgataa acttatcatc cccttttgct gatggagctg cacatgacca 65100

ggtttttgac gaaagt 65116

<210> 2

<211> 26

<212> DNA

<213>

<400> 2

caattgctca cgcaagagcc aagctg 26

<210> 3

<211> 20

<212> DNA

<213>

<400> 3

tacgtggccg agtacgagca 20

<210> 4

<211> 14183

<212> DNA

<213>

<400> 4

ttcataggga attgctcacg caagagccaa gctgagttcg tctgcgtgtc ctgcggtttc 60

acctgcaacg ctgacctgaa cgcctcgatc aacgtcgcgg caggacatgc cggagggaca 120

acaacgaccc ctggtcgttc acgtgtccgt gaacctcaac tcgccctccg gtgagttgga 180

atcccccgcc tccaggcgag ggaggatgtc aaatccagaa gacaccggtg gtcttcgacg 240

gggagctgac gctgctgccc atggggcagt acctgcaccg cgccctcggc ggcgactacg 300

tcgccctcgc cgccacgcac accggcacca gcgcccccga gatagagctc gacgacagca 360

gcgacagcgg cttcgccgtt cgggaggtgg atctgcccgc accggaggac ggaagcatcg 420

aggccgcggc cgtcgcggcg cgcatcggaa cgggcctcgt cgacctgcgc gcgcaagctc 480

ccggcctgga ccggatccgc tcccagagca cgtggatgcg gacgccgctg cgcgacgcgt 540

tcgacgccgt gctcactgtt cccaccgcca ccgcggaagt ctcgacaccc cgtccggcgc 600

gcgactaagc tcaagatcgc gacgcgtgtt cgccgacgtg gaaacgacct gctgcgcgag 660

gagaacgttg accaccatcg acgaagttcc cggcatggcc gacgaaaccg cactcctcga 720

ctggctgggc acgatgcgcg agaagcagcc ggtgtggcag gaccggtacg gcgtgtggca 780

cgtcttccgc cacgccgacg tccagacggt gttgcgcgac accgccacgt tctcctccga 840

cccgacccgc gtcatcgagg gcgcgagccc gacgccgggc atgatccacg agatcgaccc 900

gccggagcac cgcgccctgc gcaaggtcgt cagcagcgcc ttcaccccgc gcaccatctc 960

cgacctcgaa ccccgcatcc gcgacgtcac ccgctcgctg ctggccgatg ccggcgagag 1020

cttcgacctc gtcgacgtgc tcgcgttccc gctgccggtc acgatcgtcg cggagctgct 1080

gggcctgccg ccgatggacc acgagcagtt cggcgactgg tcgggtgccc tggtcgacat 1140

ccagatggac gacccgaccg acccggcgct ggccgaacgg atcgcggatg tgctcaaccc 1200

gctcaccgcg tacctgaagg cccgctgcgc cgagcgccgc gccgatcccg gcgacgacct 1260

gatctcccgc cttgtcctgg ccgaggtcga cgggcgcgcg ctcgacgacg aggaggcggc 1320

caacttctcc accgcgctgc tgctggccgg ccacatcacc acgaccgtcc tgctcggcaa 1380

catcgtgcgc accctcgacg agcacccggc ccactgggac gccgccgccg aggaccccgg 1440

ccggatcccg gcgatcgtcg aggaggtcct gcgctaccgg ccgccgttcc cgcagatgca 1500

gcgcacgacg accaaggcca ccgaggtcgc gggcgtgccc atcccggccg acgtcatggt 1560

caacacctgg gtgctctcgg ccaaccgcga ctccgacgcc cacgacgacc ccgaccggtt 1620

cgacccgtcg cgcaagtccg gcggcgccgc gcagctctcc ttcggccacg gcgtgcactt 1680

ctgcctcggc gcgcccctgg cccggctgga gaaccgcgtg gcgctggagg agatcatcgc 1740

ccggttcggg cggctcaccg tcgaccgcga cgacgagcgc ctgcgccact tcgaacagat 1800

cgtgctcggg acccgtcacc tgccggtgct ggcgggctcc tcgccgaggc agtcggcgta 1860

gtcgggcagc atccctcgcc gcctggcggt ctccaggtcc acggccaccg tgtcgcggtc 1920

ggagagcacg acctccaggt ccgcgggcca cggcagaccc agcgcggggt cgaacgggtc 1980

gatcgcctgc tcgtactcgg ccacgtaaat cgtcgacctg caggcatgca agcttggcgt 2040

aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 2100

tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 2160

taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 2220

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 2280

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 2340

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 2400

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 2460

tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 2520

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 2580

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 2640

ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 2700

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 2760

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 2820

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 2880

acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 2940

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3000

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3060

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3120

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 3180

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 3240

cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 3300

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 3360

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 3420

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 3480

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 3540

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 3600

catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 3660

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 3720

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 3780

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 3840

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 3900

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 3960

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 4020

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 4080

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 4140

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 4200

acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 4260

cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 4320

agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 4380

cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 4440

tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 4500

tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 4560

cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 4620

cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta 4680

cccggggatc ctctagctag agtcgacctg caggtccccg gggtgggcga agaactccag 4740

catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc 4800

caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc 4860

ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag 4920

aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc 4980

cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg 5040

tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg 5100

atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc 5160

gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca 5220

tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct 5280

tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc 5340

atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact 5400

tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa 5460

ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg 5520

gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac 5580

acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc 5640

acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct 5700

catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa 5760

gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat 5820

tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg 5880

caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg 5940

acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt 6000

tagcagccct tgcgccctga gtgcttgcgg cagcgtcgcc attgatgcgg gccagctcgc 6060

ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt 6120

aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg 6180

gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca 6240

tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc 6300

cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg 6360

ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac 6420

gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg 6480

ctataatgac cccgaagcag ggttatgcag cggaaaagat ccgtcgacct gcaggcatgc 6540

tggcgccgga cggggcttca gacgtttcgg gtgctgggtt gttgtctctg gacagtgatc 6600

catgggaaac tactcagcac caccaatgtt cccaaaagaa agcgcaggtc agcgcccatg 6660

agccaagatc taggcatgtc gcccttcatc gctcccgacg tccctgagca ccttctagca 6720

gaattaccct ccacgttgat tgtctgcgag gcaagaatga tcatcaccgt agtgagagtg 6780

cgttcaaggc tcttgcggtt gccataagag aagccacctc gcccaatggt accaacgatg 6840

ttccctccac caaaggtgtt cttatgtagt gacaccgatt atttaaagct gcagcatacg 6900

atatatatac atgtgtatat atgtatacct atgaatgtca gtaagtatgt atacgaacag 6960

tatgatactg aagatgacaa ggtaatgcat cattctatac gtgtcattct gaacgaggcg 7020

cgctttcctt ttttcttttt gctttttctt tttttttctc ttgaactcga cggatctatg 7080

cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg 7140

ttaatctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa 7200

gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc 7260

gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt 7320

taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt 7380

gatccggaac ccttaatata actccgtata atggatgcta tacgaggtta ttaggtccct 7440

cgactatagg gtcaccgtcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg 7500

ccggcacggc ctgggtaacc aggtattttg tccacataac cgtgcgcaaa atgttgtgga 7560

taagcaggac acagcagcaa tccacagcag gcatacaacc gcacaccgag gttactccgt 7620

tctacaggtt acgacgacat gtcaatactt gcccttgaca ggcattgatg gaatcgtagt 7680

ctcacgctga tagtctgatc gacaatacaa gtgggaccgt ggtcccagac cgataatcag 7740

accgacaaca cgagtgggat cgtggtccca gactaataat cagaccgacg atacgagtgg 7800

gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gttccagact 7860

aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc agaccgacga 7920

tacgagtggg accatggtcc cagactaata atcagaccga cgatacgagt gggaccgtgg 7980

tcccagtctg attatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca 8040

gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg 8100

ggaccgtggt cccagtctga ttatcagacc gacgatacaa gtggaacagt gggcccagag 8160

agaatattca ggccagttat gctttctggc ctgtaacaaa ggacattaag taaagacaga 8220

taaacgtaga ctaaaacgtg gtcgcatcag ggtgctggct tttcaagttc cttaagaatg 8280

gcctcaattt tctctataca ctcagttgga acacgagacc tgtccaggtt aagcaccatt 8340

ttatcgccct tatacaatac tgtcgctcca ggagcaaact gatgtcgtga gcttaaacta 8400

gttcttgatg cagatgacgt tttaagcaca gaagttaaaa gagtgataac ttcttcagct 8460

tcaaatatca ccccagcttt tttctgctca tgaaggttag atgcctgctg cttaagtaat 8520

tcctctttat ctgtaaaggc tttttgaagt gcatcacctg accgggcaga tagttcaccg 8580

gggtgagaaa aaagagcaac aactgattta ggcaatttgg cggtgttgat acagcgggta 8640

ataatcttac gtgaaatatt ttccgcatca gccagcgcag aaatatttcc agcaaattca 8700

ttctgcaatc ggcttgcata acgctgacca cgttcataag cacttgttgg gcgataatcg 8760

ttacccaatc tggataatgc agccatctgc tcatcatcca gctcgccaac cagaacacga 8820

taatcacttt cggtaagtgc agcagcttta cgacggcgac tcccatcggc aatttctatg 8880

acaccagata ctcttcgacc gaacgccggt gtctgttgac cagtcagtag aaaagaaggg 8940

atgagatcat ccagtgcgtc ctcagtaagc agctcctggt cacgttcatt acctgaccat 9000

acccgagagg tcttctcaac actatcaccc cggagcactt caagagtaaa cttcacatcc 9060

cgaccacata caggcaaagt aatggcatta ccgcgagcca ttactcctac gcgcgcaatt 9120

aacgaatcca ccatcggggc agctggtgtc gataacgaag tatcttcaac cggttgagta 9180

ttgagcgtat gttttggaat aacaggcgca cgcttcatta tctaatctcc cagcgtggtt 9240

taatcagacg atcgaaaatt tcattgcaga caggttccca aatagaaaga gcatttctcc 9300

aggcaccagt tgaagagcgt tgatcaatgg cctgttcaaa aacagttctc atccggatct 9360

gacctttacc aacttcatcc gtttcacgta caacattttt tagaaccatg cttccccagg 9420

catcccgaat ttgctcctcc atccacgggg actgagagcc attactattg ctgtatttgg 9480

taagcaaaat acgtacatca ggctcgaacc ctttaagatc aacgttcttg agcagatcac 9540

gaagcatatc gaaaaactgc agtgcggagg tgtagtcaaa caactcagca ggcgtgggaa 9600

caatcagcac atcagcagca catacgacat taatcgtgcc gatacccagg ttaggcgcgc 9660

tgtcaataac tatgacatca tagtcatgag caacagtttc aatggccagt cggagcatca 9720

ggtgtggatc ggtgggcagt ttaccttcat caaatttgcc cattaactca gtttcaatac 9780

ggtgcagagc cagacaggaa ggaataatgt caagccccgg ccagcaagtg ggctttattg 9840

cataagtgac atcgtccttt tccccaagat agaaaggcag gagagtgtct tctgcatgaa 9900

tatgaagatc tggtacccat ccgtgataca ttgaggctgt tccctggggg tcgttacctt 9960

ccacgagcaa aacacgtagc cccttcagag ccagatcctg agcaagatga acagaaactg 10020

aggttttgta aacgccgcct ttatgggcag caaccccgat caccggtgga aatacgtctt 10080

cagcacgtcg caatcgcgta ccaaacacat cacgcatatg attaatttgt tcaattgtat 10140

aaccaacacg ttgctcaacc cgtcctcgaa tttccatatc cgggtgcggt agtcgccctg 10200

ctttctcggc atctctgata gcctgagaag aaaccccaac taaatccgct gcttcaccta 10260

ttctccagcg ccgggttatt ttcctcgctt ccgggctgtc atcattaaac tgtgcaatgg 10320

cgatagcctt cgtcatttca tgaccagcgt ttatgcactg gttaagtgtt tccatgagtt 10380

tcattctgaa catcctttaa tcattgcttt gcgttttttt attaaatctt gcaatttact 10440

gcaaagcaac aacaaaatcg caaagtcatc aaaaaaccgc aaagttgttt aaaataagag 10500

caacactaca aaaggagata agaagagcac atacctcagt cacttattat cactagcgct 10560

cgccgcagcc gtgtaaccga gcatagcgag cgaactggcg aggaagcaaa gaagaactgt 10620

tctgtcagat agctcttacg ctcagcgcaa gaagaaatat ccaccgtggg aaaaactcca 10680

ggtagaggta cacacgcgga tagccaattc agagtaataa actgtgataa tcaaccctca 10740

tcaatgatga cgaactaacc cccgatatca ggtcacatga cgaagggaaa gagaaggaaa 10800

tcaactgtga caaactgccc tcaaatttgg cttccttaaa aattacagtt caaaaagtat 10860

gagaaaatcc atgcaggctg aaggaaacag caaaactgtg acaaattacc ctcagtaggt 10920

cagaacaaat gtgacgaacc accctcaaat ctgtgacaga taaccctcag actatcctgt 10980

cgtcatggaa gtgatatcgc ggaaggaaaa tacgatatga gtcgtctggc ggcctttctt 11040

tttctcaatg tatgagaggc gcattggagt tctgctgttg atctcattaa cacagacctg 11100

caggaagcgg cggcggaagt caggcatacg ctggtaactt tgaggcagct ggtaacgctc 11160

tatgatccag tcgattttca gagagacgat gcctgagcca tccggcttac gatactgaca 11220

cagggattcg tataaacgca tggcatacgg attggtgatt tcttttgttt cactaagccg 11280

aaactgcgta aaccggttct gtaacccgat aaagaaggga atgagatatg ggttgatatg 11340

tacactgtaa agccctctgg atggactgtg cgcacgtttg ataaaccaag gaaaagattc 11400

atagcctttt tcatcgccgg catcctcttc agggcgataa aaaaccactt ccttccccgc 11460

gaaactcttc aatgcctgcc gtatatcctt actggcttcc gcagaggtca atccgaatat 11520

ttcagcatat ttagcaacat ggatctcgca gataccgtca tgttcctgta gggtgccatc 11580

agattttctg atctggtcaa cgaacagata cagcatacgt ttttgatccc gggagagact 11640

atatgccgcc tcagtgaggt cgtttgactg gacgattcgc gggctatttt tacgtttctt 11700

gtgattgata accgctgttt ccgccatgac agatccatgt gaagtgtgac aagtttttag 11760

attgtcacac taaataaaaa agagtcaata agcagggata actttgtgaa aaaacagctt 11820

cttctgaggg caatttgtca cagggttaag ggcaatttgt cacagacagg actgtcattt 11880

gagggtgatt tgtcacactg aaagggcaat ttgtcacaac accttctcta gaaccagcat 11940

ggataaaggc ctacaaggcg ctctaaaaaa gaagatctaa aaactataaa aaaaataatt 12000

ataaaaatat ccccgtggat aagtggataa ccccaaggga agttttttca ggcatcgtgt 12060

gtaagcagaa tatataagtg ctgttccctg gtgcttcctc gctcactcga gggcttcgcc 12120

ctgtcgctca actgcggcga gcactactgg ctgtaaaagg acagaccaca tcatggttct 12180

gtgttcatta ggttgttctg tccattgctg acataatccg ctccacttca acgtaacacc 12240

gcacgaagat ttctattgtt cctgaaggca tattcaaatc gttttcgtta ccgcttgcag 12300

gcatcatgac agaacactac ttcctataaa cgctacacag gctcctgaga ttaataatgc 12360

ggatctctac gataatggga gattttcccg actgtttcgt tcgcttctca gtggataaca 12420

gccagcttct ctgtttaaca gacaaaaaca gcatatccac tcagttccac atttccatat 12480

aaaggccaag gcatttattc tcaggataat tgtttcagca tcgcaaccgc atcagactcc 12540

ggcatcgcaa actgcacccg gtgccgggca gccacatcca gcgcaaaaac cttcgtgtag 12600

acttccgttg aactgatgga cttatgtccc atcaggcttt gcagaacttt cagcggtata 12660

ccggcataca gcatgtgcat cgcataggaa tggcggaacg tatgtggtgt gaccggaaca 12720

gagaacgtca caccgtcagc agcagcggcg gcaaccgcct ccccaatcca ggtcctgacc 12780

gttctgtccg tcacttccca gatccgcgct ttctctgtcc ttcctgtgcg acggttacgc 12840

cgctccatga gcttatcgcg aataaatacc tgtgacggaa gatcacttcg cagaataaat 12900

aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga 12960

cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 13020

cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 13080

cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 13140

tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt 13200

aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg 13260

cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc tggtgatatg 13320

ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct 13380

ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc 13440

gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt 13500

ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa 13560

cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat 13620

gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct 13680

taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag 13740

ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa 13800

tggcagaaat tcgatgataa gctgtcaaac atgagaattg gtcgacggcg cgccaaagct 13860

tgcatgcctg cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc 13920

cctgcgtaag cggggcacat ttcattacct ctttctccgc acccgacata gataataact 13980

tcgtatagta tacattatac gaagttatct agtagactta attaaggatc gatccggcgc 14040

gccaatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg 14100

catcggtcga gcttgacatt gtaggactat attgctctaa taaatttgcg gccgctaata 14160

cgactcacta tagggagagg atc 14183

<210> 5

<211> 58

<212> DNA

<213>

<400> 5

gagctggatc atctggatca ctttcgtcaa aaacctggtc atgtgcagct ccatcagc 58

<210> 6

<211> 26

<212> DNA

<213>

<400> 6

gaattcggcg accctacgcc cccaac 26

<210> 7

<211> 24

<212> DNA

<213>

<400> 7

gctagcggag tgtatactgg ctta 24

<210> 8

<211> 19

<212> DNA

<213>

<400> 8

ctagaaatat tttatctga 19

<210> 9

<211> 26

<212> DNA

<213>

<400> 9

tctagaactc cagctgcgtg accatc 26

<210> 10

<211> 26

<212> DNA

<213>

<400> 10

caattgactc cacgagtcgt tccagg 26

<210> 11

<211> 5329

<212> DNA

<213>

<400> 11

ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt 60

catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat 120

attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa 180

tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag 240

tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga 300

aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt 360

tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat 420

tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag 480

ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat 540

ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag 600

ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact 660

gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg 720

gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa 780

gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat 840

ttctagaact ccagctgcgt gaccatcgtc gccgtctcga tcgtcggcgc ctccaggcac 900

cagtccacga tcgccgaggt gatgccgtag gggatcgcgc cgacgaaggc gaacggctcg 960

ggcggcggtt cggcggtgag gaagtcggcg ttgacgactt cgatgttcgg gtggccggaa 1020

agcttctccc gcaacgactt cgccagccgg gggtcgatct cgtacgacgt cacctgacgc 1080

gcgcggtcgg cgagttccct ggtgagcagc ccttcgccgg ggccggcttc cagcaccggc 1140

agatcgggcc gcagctcggc tgtctcggcg atgcgcgcga tggtcttgcg gtcgcggagg 1200

aagttctgcc cgaactggcg ccggttgcgg tcccgctcgg tacggcccag caccggccgg 1260

ttctggttgg ggtgctgccg atcctggttg cggcgacgcg ggcgcggctg ctcgtccgaa 1320

ctgctcaccg ctggatccta ccaaccggca cgattgtcca gcccacaaca gcatcgcggt 1380

gccacgtgtg gaccgcgtcg gtcagatcct ccccgcacct ctcgccagcc gtcaagatcg 1440

accgcgtgca cctgcgatcg ccgatcaacc gcgactagca tcgggcgcaa gccgccactc 1500

gaacggacac tcgcatggac gtccccttcc tggacctgca agccgcgtac ctcgaactcc 1560

ggtccgacat cgaccaggcg tgccggcgcg tgctcgggtc gggctggtac ctgcacgggc 1620

cggagaacga ggcgttcgag gcggagttcg ccgcctactg cgagaacgcg cactgcgtga 1680

cggtcggcag cggatgcgac gcgctggagc tgagcctgct ggcgctgggc gtcggtcagg 1740

gcgacgaggt gatcgtgccg tcccacacct tcatcgcgac gtggctcgcg gtgtcccgcg 1800

tgggcgcggt gccggtgcca gtcgaaccgg aaggcgtgag ccacacgctc gatccggccc 1860

tggtggaaca ggccatcacg ccgcggacgg ccgccatcct gccggtgcac ctctacggcc 1920

accctgcgga cctcgacgcg ctgcgagcga tcgccgaccg ccacggcctg gcactggtgg 1980

aggacgtcgc acaggcggtc ggcgcccgcc accgcggcca ccgcgtcggc gccggctcga 2040

acgccgcggc cttcagcttc tacccgggca agaacctcgg agcgctgggc gacggcggcg 2100

cggtcgtcac caccgacccg gccctggccg agcgcatccg cctgctgcgc aactacggct 2160

cgaagcagaa gtacgtgcac gaggtccgcg gcaccaacgc ccgcctcgac gaactccagg 2220

cggcggtcct gcgcgtcaag ctccgccacc tcgacgactg gaacgcccgc cgcacaacgc 2280

tggcgcagca ctaccagacg gaactgaagg acgtccccgg catcacgctc ccggaaaccc 2340

acccctgggc cgactcagcc tggcacctct tcgtcctccg ctgcgagaac cgcgaccacc 2400

tccaacgcca cctgaccgac gcgggcgtcc agaccctcat ccactacccg accccggtcc 2460

acctctcacc cgcctacgcc gacctcggcc tgcccccagg ctccttcccc gttgcggagt 2520

ctctggcggg ggaggtcctc agcctcccca tcggccccca cctctccagg gaggcggcag 2580

accacgtcat cgcgaccctc aaggctgggg cttgatctgc gttcggctca cgcgcagtgg 2640

aagcacaccc gccgcgcaat ccgcgcccgg tccgctcccg cgttcaccgg gatcgcttcc 2700

ccggtcttga tccgctccca agcgttgggt tcgtcgtgga tttcgcccca ctcatcgaag 2760

ctctccccgt agtggttgca cttcccgggc aggtgggccg tgtccgctcg atacggtccc 2820

gcggagatcg acacgtgggg agggttcgat gacgtcgccg ggcggagacg acggcggtgg 2880

caggcacccg gtcgcggaga ccggtggtga accggtcgcg gaggtgttca tcccggctca 2940

agcgggtgcg gacggcaggc aggcccagtt ggagctgcgc cagacgaccg ggggcgcgct 3000

cgcgctggtg ctctacacga gcctggaacg actcgtggag tcaattcggc gaccctacgc 3060

ccccaactga gagaactcaa aggttacccc agttggggca ctactcccga aaaccgcttc 3120

tgacctggga aaacgtgaag ccccggggca tccgctgagg gttgccgccg gggcttcggt 3180

gtgtccgtca gtacgggcca tagaggggcg tcgtgtaacc cacccagggc gattgctccg 3240

gcacggggaa gccgcgccac gccttcggga cgtctggaat cgctagagct tgcatgcctg 3300

caggtcgacg gatcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt 3360

cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact 3420

ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga 3480

gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg 3540

ctctgcgagg ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg 3600

aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac 3660

gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg 3720

ccgtcggcca gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg 3780

cccgcatcaa tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg 3840

acccgcgcac ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag 3900

agaagcagga cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat 3960

gactttttta gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg 4020

cgctccatca agaagagcga cttcgcggag ctggtgaagt acatcaccga cgagcaaggc 4080

aagaccgatc cccggggacc tgcaggtcga ctctagctag ctggccaggg cccgatacgt 4140

cgcggtgagt tcaggctttt tcatatctca ttgcccccgg acgagcgtct gctccgccat 4200

tcgccgtccg ccgtgccaat cggatcagcc gtccaaatgc gggattttcg ttagtcggag 4260

gccaaacggc attgagcgtc agcatatcat cagcgagctg aagaaagaca atccccgatc 4320

cgctccacgt gttgccccag caatcagcgc gaccttgccc ctccaacgtc atctcgttct 4380

ccgctcatga gctcagccaa tcgactggcg agcggcatcg cattcttcgc atcccgcctc 4440

tggcggatgc aggaagatca acggatctcg gcccagttga cccagggctg tcgccacaat 4500

gtcgcgggag cggatcaacc gagcaaaggc atgaccgact ggaccttcct tctgaaggct 4560

cttctccttg agccacctgt ccgccaaggc aaagcgctca cagcagtggt cattctcgag 4620

ataatcgacg cgtaccaact tgccatcctg aagaatggtg cagtgtctcg gcaccccata 4680

gggaaccttt gccatcaact cggcaagatg cagcgtcgtg ttggcatcgt gtcccacgcc 4740

gaggagaagt acctgcccat cgagttcatg gacacgggcg accgggcttg caggcgagtg 4800

aggtggcagg ggcaatggat cagagatgat ctgctctgcc tgtggccccg ctgccgcaaa 4860

ggcaaatgga tgggcgctgc gctttacatt tggcaggcgc cagaatgtgt cagagacaac 4920

tccaaggtcc ggtgtaacgg gcgacgtggc aggatcgaac ggctcgtcgt ccagacctga 4980

ccacgagggc atgacgagcg tccctcccgg acccagcgca gcacgcaggg cctcgatcag 5040

tccaagtggc ccatcttcga ggggccggac gctacggaag gagctgtgga ccagcagcac 5100

accgccgggg gtaaccccaa ggttgagaag ctgaccgatg agctcggctt ttcgccattc 5160

gtattgcacg acattgcact ccaccgctga tgacatcagt cgatcatagc acgatcaacg 5220

gcactgttgc aaatagtcgg tggtgataaa cttatcatcc ccttttgctg atggagctgc 5280

acatgaccag gtttttgacg aaagtgatcc agatgatcca gctcaatct 5329

Claims (3)

1. plasmid pZB201, its sequence such as SEQ IDNo. shown in 1.

2. the cloning process of a microbiotic synthetic gene bunch is characterized in that concrete steps are: at first make up two homologous recombination vectors of target gene bunch upstream and downstream, the site that φ BT1 intergrase is identified AttBWith AttPBe inserted in respectively the both sides of target gene bunch; By the integrase mediated locus specificity recombining reaction of φ BT1, make the gene cluster recirculation, thereby finish the clone of gene cluster again.

3. the cloning process of microbiotic synthetic gene according to claim 2 bunch is characterized in that, the microbiotic synthetic gene bunch is erythromycin, and concrete steps are as follows:

(1) structure of erythromycin gene cluster upstream homologous recombination vector pEry-up

With red saccharopolyspora Saccharopolyspora erythraeThe genome of a is template, be the erythromycin gene cluster upstream homology arm fragment of 1993bp with primer SEQ ID No. 2 and primer SEQ ID No. 3 amplification sizes, be the T-A clone with the pMD19-T carrier, obtain plasmid T-ery-up, choose the contiguous direction in XbaI site on MunI site and the T carrier, plasmid pTARBAC2.1 and plasmid pDZY101 carry out double digestion with NheI, EcoRI and XbaI, EcoRI respectively, producing size is two bar segment of 7478bp and 4178bp, connect the purpose fragment, obtain plasmid pBAC-DZY101; Plasmid pBAC-DZY101 digests with NheI and EcoRI again, and producing size is the purpose fragment of 9500bp; Plasmid T-ery-up digests with MunI and XbaI, and the generation size is 4683 purpose fragment, connects the fragment of two clauses and subclauses, obtains plasmid pEry-up, and sequence is shown in SEQ ID No. 4;

(2) homology of carrier pEry-up is inserted

The carrier pEry-up that builds is converted into intestinal bacteria ET12567, picking positive transformant and red saccharopolyspora Saccharopolyspora erythraeA carries out conjugal transfer, behind 30 ℃ of cultivation 16 ~ 20h, covers the sterilized water that lml contains nalidixic acid and kantlex at planar surface, cultivates picking zygote more than 5 days for 30 ℃;

(3) structure of erythromycin gene cluster downstream homologous recombination vector pEry-down

Take plasmid pSET152 as template, be the fragment of 2283bp with primer SEQ ID No. 5 and primer SEQ No. 6 amplification sizes, be the T-A clone with the pMD19-T carrier and obtain plasmid pT-SET152; Take plasmid pCY104 as template, be the fragment of 847bp with primer SEQ ID No. 7 and primer SEQ ID No. 8 amplification sizes, be T-A clone with the pMD19-T carrier, obtain plasmid pT-CY104, select the direction of HindIII site away from the T carrier of NheI site on the PCR product fragment; Respectively plasmid pT-SET152 and plasmid pT-CY104 are carried out double digestion with restriction enzyme HindIII, XbaI and HindIII, NheI again, producing size is the fragment of two clauses and subclauses of 2315bp and 870bp, obtains plasmid pCY104-SET152 after the connection; With red saccharopolyspora Saccharopolyspora erythraeThe genome of a is template, is the erythromycin gene cluster downstream homology arm fragment of 2194bp with primer SEQ ID No. 9 and primer SEQ ID No. 10 amplification sizes, is the T-A clone with the pMD19-T carrier, obtains plasmid pT-ery-down; Plasmid pT-ery-down and pCY104-SET152 further carry out respectively double digestion with restriction enzyme XbaI, MunI and XbaI, EcoRI again, producing size is the fragment of two clauses and subclauses of 2200bp and 3129bp, obtain plasmid pEry-down after the connection, sequence is shown in SEQ ID No. 11;

(4) homology of carrier pEry-down is inserted

The carrier pEry-down that builds is converted into intestinal bacteria ET12567, picking positive transformant and the red saccharopolyspora that has inserted upstream homologous vector pEry-up Saccharopolyspora erythraeA carries out conjugal transfer, behind 30 ℃ of cultivation 16 ~ 20h, covers the sterilized water that lml contains nalidixic acid and apramycin at planar surface, cultivates picking zygote more than 5 days for 30 ℃;

(5) clone of the erythromycin synthetic gene that mediates of the extracting of red saccharopolyspora chromogene group and external φ BT1 intergrase bunch

The red saccharopolyspora genome of homologous vector pEry-up and pEry-down has been inserted in extracting, guarantees that genomic fragment comprises whole erythromycin gene cluster sequences; 30 ° of C in specific buffer are incubated overnight with genomic fragment and φ BT1 intergrase; Under the effect of φ BT1 intergrase, erythromycin gene cluster both sides AttBWith AttPThe locus specificity recombining reaction occurs in the site, and then obtains comprising the complete sequence of erythromycin synthetic gene bunch, is designated as plasmid pZB201, its sequence such as SEQ IDNo. shown in 1.

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