CN102680290B - A kind of flaking method of automatic making liquid basal cell - Google Patents

Abstract

The present invention relates on a kind of pathology slide making apparatus used in pathological examination, for automatically making the flaking method of liquid basal cell slide.By isolated cell, filter sedimentation, extract cell liquid, centrifugal flaking step, can carry out automatic film-making on an equipment, its automaticity is high, save time, saving of labor, fast, efficient.Film-making quantity is flexible, multiple slide of can producing once, and applied widely, determine cell extraction amount automatically and accurately, cellular layer is thin, and consistance is good, clean background, ensure that the cell transfer uniform amount on slide and consistance.

Description

A kind of flaking method of automatic making liquid basal cell

Technical field

The present invention relates on a kind of pathology slide making apparatus used in pathological examination, for automatically making the flaking method of liquid basal cell slide.

Background technology

In prior art, liquid-based cell sample manufacturing has many methods.As multiple methods such as membrane type transfer printing, centrifugal smear method, natural sedimentations.For realizing these methods, useful various equipment carries out matching used, after first carrying out cell liquid centrifugal settling with hydro-extractor, then is placed into and pelleter carries out cell breaks up separation, carrying out centrifugal settling by manually taking out cell liquid again, needing the manual operation carried out repeatedly.Also have carry out on hydro-extractor centrifugal after, carry out cell by manual operation and break up separation, and then carry out film-making through natural subsidence.Have plenty of with the film-making of cell filtration transfer modes.These flaking method quality are very large by artificial affecting, are easy to because the excessive cellular morphology that causes of normal pressure is subject to squeeze distortion, not easily inspections and examinations; Have plenty of equipment composition more, consumptive material composition is many, cost is also high; And film-making can only the making of monolithic, work consuming consuming time, work efficiency is low.

Summary of the invention

Object of the present invention, will provide a kind of flaking method that automatically can make liquid basal cell slide quickly and easily on an equipment exactly.Technical scheme of the present invention is such: a kind of flaking method automatically making liquid basal cell slide in production facility, it is characterized in that carrying out according to the following steps:

A isolated cell, repeatedly to extract cell liquid with Cell Disruptor and discharges, being carried out breaing up separation by cell agglomerating for adhesion;

B filters sedimentation, is undertaken centrifugal by cell extract, makes sedimentation after cell filtration;

C extracts cell liquid, is extracted by the cell liquid after sedimentation, on the pathology slide in instillation preparation bin;

The centrifugal film-making of d, again centrifugal, make cell precipitation in preparation bin on pathology slide, form cellular layer, be liquid basal cell slide.

During film-making, liquid basal cell extract is put into the liquid basal cell extract container that fiter membrance cylinder and cell-preservation liquid are housed, then liquid basal cell extract container is put on the centrifugal pan of production facility, under program control, the Cell Disruptor that production facility is loaded runs downwards automatically, enter into fiter membrance cylinder top in pathological cells extract container, cell-preservation liquid is pumped in Cell Disruptor, then be discharged in pathological cells extract container.So repeated multiple times, make to be wrapped up agglomerating cell by mucus and broken up out in narrow and small Cell Disruptor needle tubing; Under program, equipment starts centrifugal motor automatically, and under the influence of centrifugal force, cell, by the filter membrane of fiter membrance cylinder, is deposited to the bottom of pathological cells extract container; Cell Disruptor runs downwards again, enters into fiter membrance cylinder bottom in pathological cells extract container, extracts the cell liquid of sedimentation, on the pathology slide automatically in instillation preparation bin; Again automatically start centrifugal mechanism, make the cell in preparation bin by the effect of centrifugal gravity, the larger positive cell preferential precipitation of proportion, on pathology slide, forms uniform thin-layer cell layer, is liquid basal cell slide.The beneficial effect using method of the present invention to carry out the film-making of liquid basal cell pathology is, all programs automatically can be carried out on an equipment, and automaticity is high, save time, saving of labor, fast, efficient.Film-making quantity is flexible, multiple slide of can producing once, and applied widely, determine cell extraction amount automatically and accurately, cellular layer is thin, and consistance is good, clean background, and ensure that the cell transfer uniform amount on slide and consistance, the positive rate of institute's film-making is high.

Embodiment 1

A isolated cell, liquid basal cell extract is put into the liquid basal cell extract container that fiter membrance cylinder and cell-preservation liquid are housed, then liquid basal cell extract container is put on the centrifugal pan of production facility, under program control, the Cell Disruptor that production facility is loaded runs downwards automatically, enter into fiter membrance cylinder top in pathological cells extract container, cell-preservation liquid is pumped in Cell Disruptor, then be discharged in pathological cells extract container.So repeated multiple times, make to be wrapped up agglomerating cell by mucus and broken up separation in narrow and small Cell Disruptor needle tubing;

B filters sedimentation, and under program, automatically start centrifugal motor, under the influence of centrifugal force, cell, by the filter membrane of fiter membrance cylinder, is deposited to the bottom of pathological cells extract container;

C extracts cell liquid, Cell Disruptor runs downwards automatically again, penetrate the filter membrane of fiter membrance cylinder, enter into fiter membrance cylinder bottom in pathological cells extract container, extract the cell liquid of sedimentation, then the up return of Cell Disruptor, the automatic rotational angle of centrifugal pan, cell liquid instillation card is placed on the pathology slide in the preparation bin on centrifugal pan by Cell Disruptor;

The centrifugal film-making of d, starts centrifugal mechanism again automatically, makes the cell in preparation bin by the effect of centrifugal gravity, and the larger positive cell preferential precipitation of proportion, on pathology slide, forms uniform thin-layer cell layer, is liquid basal cell slide.

Claims (1)

1. automatically make a flaking method for liquid basal cell slide, it is characterized in that, carry out according to the following steps:

A isolated cell: liquid basal cell extract is put into the liquid basal cell extract container that fiter membrance cylinder and cell-preservation liquid are housed, then liquid basal cell extract container is put on the centrifugal pan of production facility, under program control, the Cell Disruptor that production facility is loaded runs downwards automatically, enter into fiter membrance cylinder top in pathological cells extract container, cell-preservation liquid is pumped in Cell Disruptor, be discharged in pathological cells extract container again, so repeated multiple times, make to be wrapped up agglomerating cell by mucus and broken up separation in narrow and small Cell Disruptor needle tubing,

B filters sedimentation: under program, and automatically start centrifugal motor, under the influence of centrifugal force, cell, by the filter membrane of fiter membrance cylinder, is deposited to the bottom of pathological cells extract container;

C extracts cell liquid: Cell Disruptor runs downwards automatically again, penetrate the filter membrane of fiter membrance cylinder, enter into fiter membrance cylinder bottom in pathological cells extract container, extract the cell liquid of sedimentation, then the up return of Cell Disruptor, the automatic rotational angle of centrifugal pan, cell liquid instillation card is placed on the pathology slide in the preparation bin on centrifugal pan by Cell Disruptor;

The centrifugal film-making of d, starts centrifugal mechanism again automatically, makes the cell in preparation bin by the effect of centrifugal gravity, and the larger positive cell preferential precipitation of proportion, on pathology slide, forms uniform thin-layer cell layer, is liquid basal cell slide.