CN102618521B - ATP(Adenosine Triphosphate) -dependent metalloproteinase FtsH (Filamentation temperature-sensitive H - Google Patents
ATP(Adenosine Triphosphate) -dependent metalloproteinase FtsH (Filamentation temperature-sensitive H Download PDFInfo
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Abstract
The invention relates to an ATP-dependent metalloproteinase FtsH (Filamentation temperature-sensitive H). The amino acid sequence of the metalloproteinase FtsH is SEQ ID NO:1. The ATP-dependent metalloproteinase FtsH obtained from thermophile bacteria Alicyclobacillus hesperidiums has ATP enzyme activities, potent proteolytic activities and molecular chaperone activities, and can be applicable to genetic modification to crops. The ATP-dependent metalloproteinase FtsH can be applied to the modification to gene engineering crops, better improve the environmental pressure resisting capability of the environmental pressure of the crops, and greatly increase the yield of the crops.
Description
Technical field
The invention belongs to the zymoprotein technical field, be specifically related to the metalloprotease FtsH that a kind of ATP-relies on.
Background technology
Thereby the protein in organism is often because of various forms of damage losses of function, and repairing/remove impaired albumen is a kind of form that protein quality is controlled.Molecular chaperones can assist impaired albumen again folding, and some enzymatic repair processes also can reverse the damage of some albumen, thereby the albumen that can not repair is degraded by the proteolysis system and thoroughly removed.174-263 tPA enzyme (ATPases Associated with a variety of cellular Activities) has proteolytic enzyme and chaperone activity simultaneously, can mediate degraded and the reparation of membranin in bacterium, plastosome, form the quality control system of an embrane-associated protein.
FtsH (Filamentation temperature-sensitive H) belongs to the 174-263 tPA enzyme family, is important embrane-associated protein enzyme.In vivo, FtsH forms one six poly-ring structure by oligomerization, and the proteolytic activity site is embedded in six poly-complex body hole central authorities.The conservative module of FtsH albumen comprises N-end membrane-spanning domain, AAA structure, zine ion binding modules etc.FtsH has atpase activity, and proteolytic activity and chaperone activity participate in the protein quality balancing control, also oozes with heat shock, height, the responses such as light is coerced, low temperature, disease are related.Due to the diversity of FtsH function, thus to cell in many Metabolic activities relevant with developmental process, have great importance.In recent years, its protease activity there has been certain research, but has still existed many problems to remain further to be solved.And in different species, the sequence variations of FtsH is very large, and therefore, the sequence of separation and definite FtsH is an insoluble problem always.
Summary of the invention
The object of the present invention is to provide a kind of metalloprotease FtsH that in thermophile bacteria Alicyclobacillus hesperidium, ATP-relies on that comes from, the research object under extreme life condition is provided, to replenish the deficiencies in the prior art.
The invention provides the metalloprotease FtsH that a kind of ATP-relies on, it is characterized in that: the aminoacid sequence of described enzyme is SEQ ID NO:1.
The present invention also provides a kind of Nucleotide, for the metalloprotease FtsH of the ATP-dependence of encoding above-mentioned.
The sequence of described Nucleotide is SEQ ID NO:2.
The present invention also provides recombinant plasmid, is used for expressing the metalloprotease FtsH that ATP-of the present invention relies on, and the gene order that this recombinant plasmid carries is SEQ ID NO:2.
Above-mentioned proteolytic enzyme is used for the stress reaction of research cell, can be applicable to the genetic modification of farm crop.
The present invention obtains comes from the metalloprotease FtsH that in thermophile bacteria Alicyclobacillus hesperidium, ATP-relies on, and has atpase activity, and proteolytic activity and chaperone activity can be applicable to the genetic modifications of farm crop.The metalloprotease FtsH that ATP-of the present invention relies on can be applicable to the transformation of genetically engineered farm crop, can improve better crop and resist environmental stress, greatly improves the output of farm crop.
Embodiment
Below in conjunction with example, method of the present invention is described further.But example only limits to explanation, is not limited to this.The experimental technique of unreceipted actual conditions in the following example, condition routinely usually, the condition described in " the molecular cloning experiment guide " write as J. Pehanorm Brooker (Sambrook) etc., or the condition operation of advising according to manufacturer.
Embodiment 1: the metalloprotease FtsH full length gene cDNA that ATP-of the present invention relies on obtains
The present invention at first utilize two generation sequencing technologies carry out genome sequencing, splicing and note.The gene that will have a FtsH conserved regions is caught to terminator codon UGG from initiator codon AUG.Concrete steps are:
1, the genome sequencing of Alicyclobacillus hesperidium:
The extraction of total DNA and the structure in library: the centrifugal Alicyclobacillus hesperidium thalline that obtains, carry out total DNA extraction with reference to the Fermentas GeneJET Genomic DNA Purification Kit of company.Build sequencing library with reference to KAPA NGS Library Preparation Kit.Carry out genome sequencing with the state-of-the-art Solexa paired-end of Illumina HiSeq2000, produce the data of 160 times of fraction of coverage.Use Velvet (Sequence assembler for very short reads) joining method, obtain 130 contigs.And tentatively carry out genome annotation, found approximately 1800 encoding egg white genes.
2, the metalloprotease FtsH full length gene cDNA prediction of ATP-dependence:
Bioinformatic analysis in 1800 encoding egg white genes, finds the protein sequence of the metalloprotease FtsH conservative region of ATP-dependences such as having N-end membrane-spanning domain, AAA structure, zine ion binding modules.Be the cDNA sequence of prediction to terminator codon UAA from initiator codon AUG, the long 1809bp of full-length cDNA reading frame, predict that its of coding contains 602 amino-acid residues, nucleotide sequence is as described in SEQ ID NO:1 in sequence table, and the nucleotides sequence of its corresponding gene is classified SEQ ID NO:2 as.
3, the metalloprotease FtsH full length gene cDNA of ATP-dependence obtains:
The extraction of total RNA and cDNA are synthetic: the centrifugal Alicyclobacillus hesperidium thalline that obtains, carry out total RNA with reference to the Qiagen RNeasy Mini Kit of company test kit specification sheets and extract.The amplification of cDNA full-length gene fragment adopts the Invitrogen M-MLV Reverse transcriptase of company to carry out the synthetic of the first chain, obtains 10 μ l cDNA the first chain products.
CDNA total length PCR reaction: according to the sequences Design Auele Specific Primer P1 that predicts the metalloprotease FtsH gene fragment that ATP-relies on, P2 (P1:5 '-ATGAACCGTTTTTACCGGAGC-3 ' SEQ IDNO:3; P2:5 '-TCAGCCACAGCTTTCCATGATC-3 ' SEQ ID NO:4).Get cDNA 2 μ l and be used for PCR reaction, system 50 μ l.Reaction conditions is 94 ℃ of 3min; 94 ℃ of 30sec, 60 ℃ of 30sec, 72 ℃ of 2min totally 30 circulations; 72 ℃ of 10min.Amplification obtains the fragment 1809bp of the metalloprotease FtsH gene reading frame of ATP-dependence, cuts glue recovery test kit with Omega and carries out fragment purification.PEASY-Blunt Cloning Kit according to TransGen company carries out ligation and Transformed E .coli competent cell DH5 α.Get 200 μ l conversion fluid coated plates, overnight incubation.10 positive colonies of random choose identify, deliver to the order-checking of Invitrogen company after activation and identify.
The heterogenous expression of the metalloprotease FtsH full-length cDNA that embodiment 2:Alicyclobacillus hesperidium ATP-relies on.
With the metalloprotease FtsH recombinant protein that prokaryotic expression system abduction delivering Alicyclobacillus hesperidium ATP-relies on, concrete steps are as follows:
1, the Construction and identification of recombinant plasmid FtsH-pET32a: the full length cDNA sequence of the metalloprotease FtsH gene that relies on according to Alicyclobacillus hesperidium ATP-, introduce respectively restriction endonuclease sites Sac I and Sal I site at its two ends, the metalloprotease FtsH gene that the ATP-of the present invention that increases relies on, subclone advances prokaryotic expression plasmid pET32a, and order-checking is defined as correct insertion genophore.
2, recombinant plasmid FtsH-pET32a transforms intestinal bacteria E.coli BL21 (DE3) competence, and 37 ℃ of overnight incubation in LA (containing 100 μ g/m1) flat board are by indigo plant screening transformant in vain.Choose white transformant list colony inoculation dull and stereotyped in LA, 37 ℃ of overnight incubation.Be stained with a little bacterium colony with toothpick and be PCR.PCR condition: 94 ℃ of 10min; 94 ℃ of 30sec, 60 ℃ of 30sec, 72 ℃ of 2min, totally 30 circulations; Complete amplification after 72 ℃ of 10min.1.0% agarose gel electrophoresis detects pcr amplification product.
3, metalloprotease FtsH abduction delivering and the purifying of ATP-dependence: the E.coli BL21 with recombinant plasmid FtsH-pET32a of empirical tests rules on the LA flat board, 37 ℃ of cultivations.Picking list bacterium colony access liquid LA test tube, 37 ℃ of shaking table overnight incubation.By 1% inoculum size access 100ml LA substratum, 37 ℃ of shaking table 210r/min are cultured to the dense OD of bacterium
600Reach 0.6～0.8, add IPTG to final concentration 1mM, 37 ℃ of shaking tables were cultivated 4 hours, induced the FtsH protein expression, centrifugal collection supernatant liquor.Supernatant liquor is collected the albumen elution peak through nickel ion chelating affinity chromatography column purification, and SDS-PAGE detects the protein purification situation.The elutriant that will contain target protein carries out desalination and concentration, the metalloprotease FtsH that the ATP-that acquisition Alicyclobacillus hesperidium originates relies on.
4, metalloprotease FtsH activity and the thermal stability analysis of ATP-dependence: the metalloprotease FtsH that the ATP-after purifying of the present invention is relied on is dissolved in 50mM Tris-acetate (pH8.0), and 70 ℃ of temperature were bathed 2 hours; The metalloprotease FtsH that ATP-after the metalloprotease FtsH that 2 μ g ATP-of the present invention rely on or temperature are bathed relies on respectively with 1 μ g beta-casein (Sigma), final concentration 50mM Tris-acetic acid (pH8.0), the 5mM magnesium acetate, 12.5 μ M zinc acetate, 80mM sodium-chlor, 100 μ g/mL BSA, 1.4mM beta-mercaptoethanol solution mixes, 20 μ l reaction systems add 5mMATP before reaction, 38 ℃ were reacted 2 hours.Add sample-loading buffer (0.25MTris, 1.92M glycine, 1%SDS, pH8.4-8.9) termination reaction, ice bath.Sample carries out electrophoretic separation with 12%SDS-PAGE, coomassie brilliant blue R_250 dyeing.Not add the metalloprotease FtsH that ATP-of the present invention relies on to organize as blank.Found that the reaction system that adds the metalloprotease FtsH that ATP-of the present invention relies on, the brightness of beta-casein band obviously is weaker than blank group, and the protein-active of 70 ℃ of temperature after bathing is constant.
With the metalloprotease FtsH recombinant protein that eukaryotic expression system abduction delivering Alicyclobacillus hesperidium ATP-relies on, concrete steps are as follows:
1, the Construction and identification of recombinant plasmid pROK2-FtsH: the full length cDNA sequence of the metalloprotease FtsH gene that relies on according to Alicyclobacillus hesperidium ATP-, introduce respectively restriction endonuclease sites Kpn I and Sac I site at its two ends, the metalloprotease FtsH gene that the ATP-of the present invention that increases relies on, subclone advances eukaryon expression plasmid pROK2, and order-checking is defined as correct insertion genophore.
2, freeze-thaw method is converted into agrobacterium tumefaciens lba4404 with recombinant plasmid pROK2-FtsH, cultivates 2-3d for 28 ℃ in YEB (containing 50mg/L kantlex and 125mg/L Rifampin) flat board, the screening transformant.The single colony inoculation of the Agrobacterium that picking transforms is in YEB liquid nutrient medium (containing 50mg/L kantlex and 125mg/L Rifampin), and 28 ℃ of 220rpm shake training 16 hours, are PCR with bacterium liquid.PCR condition: 94 ℃ of 5min; 94 ℃ of 30sec, 60 ℃ of 1min, 72 ℃ of 2min, totally 35 circulations; Complete amplification after 72 ℃ of 10min.1.0% agarose gel electrophoresis detects pcr amplification product.
3, the genetic transformation of paddy rice: get positive Agrobacterium and be seeded on the YEB solid medium, cultivated 2 days for 28 ℃.Single bacterium colony access 5ml YEB liquid nutrient medium, 220rpm shakes training 24 hours, and centrifugal 10 minutes of 5000rpm abandons supernatant.It is resuspended that 20ml contains the AAM substratum of 100 μ M Syringylethanones, acutely shakes, and adjusts bacterial concentration OD
600Be 0.1-1.0 left and right, static 1 hour.The prematurity fringe of 15-20 days after water intaking rice pollination, the seed rear cleaning and sterilizing of peeling off.Choose rataria and be inoculated in inducing culture, 27 ℃ of dark cultivations 10-14 days.Wait for that callus grows up follow-up generation, select the embryo callus after the third generation, add the Agrobacterium bacterium liquid of above-mentioned processing, slightly shook rear static 30 minutes, be inoculated in common substratum after drying callus on aseptic filter paper, 25 ℃ of dark cultivations 3 days.The callus that picking is cultivated altogether is in wide-mouth culturing bottle, sterile water wash.Use at last standing one hour of the sterilized water of 250mg/L penbritin, dry.Second day goes to selects substratum (the 500mg/L piperazine draws for 250mg/L penbritin, 1mg/L ZT) screening kanamycin-resistant callus tissue.
4, the cultivation of transgenic paddy rice and observation: every two weeks are transferred to callus on new selection substratum, and approximately to need for three weeks be visible warty resistant calli grows from the shrivelled callus of brownization.The resistant calli of color cadmium yellow is transferred on division culture medium 3-5 days.Select a part and go on division culture medium, put out new shoots and root after 3 weeks.Seedling is moved on root media, and seedling is used for the analyses such as thermotolerance.
5, transgenic paddy rice and blank group Analysis of Heat Tolerance: the transgenic paddy rice group and the blank group of the transgenosis plant while in 42 ℃ of pre-treatment 2 hours, further 50 ℃ of illumination box heat shocks were processed 8 hours, the seedling of heat stress is moved to recover growth under the normal growth condition subsequently; Recover to observe the plant phenotype after 2 weeks.After result showed for 2 weeks, containing the blank group of metalloprotease FtsH transgenic paddy rice group that the ATP-in Alicyclobacillus hesperidium of the present invention source relies on restore normal growth substantially, and blank group damage is heavier, blank group plant be described more easily come to harm than the metalloprotease FtsH transgenic paddy rice group plant of the ATP-dependence that contains Alicyclobacillus hesperidium of the present invention and originate.
6, contain Chlorophyll Fluorescence analysis under metalloprotease FtsH transgenic paddy rice that the ATP-in Alicyclobacillus hesperidium of the present invention source relies on and blank group high temperature stress: the resistance seedling is after 6 weeks of growth, carry out 48 ℃, 50 ℃ high temperature stresss in the constant temperature illumination box and process, carry out subsequently renewal cultivation under the normal growth condition.Before processing respectively at heat shock, heat shock process after, recover 1 day, recover to detect 1 maximum chlorophyll fluorescence parameters of launching leaf with the Portable fluorescence determinator in 2 days, comprise initial fluorescence (F
0), maximum fluorescence (F
m), variable fluorescence (F
v), the Photochemical Efficiency (F of chloroplast(id)
v/ F
m).Before measuring, dark adatpation is 20 minutes, first irradiating and detecting light (＜0.05 μ mol/m during mensuration
2/ s), then shine saturation pulse light (12000 μ mol/m
2/ s).Every kind of transgenic line is selected respectively resistant plant 5 strains, selects the blank strain of equivalent amount, carries out various heat shocks and processes.Experimental result shows, through 2 day decubation, the F of transgenic rice plant
v/
mThe basic recovery normally, and in blank group plant, F
v/ F
mStill be in lower level, show under high temperature stress, blank group plant has suffered the more serious injury of metalloprotease FtsH transfer-gen plant than the ATP-dependence that contains Alicyclobacillus hesperidium of the present invention source.
Claims (5)
1. the metalloprotease FtsH that relies on of an ATP-, it is characterized in that: the aminoacid sequence of described enzyme is SEQ ID NO:1.
2. Nucleotide, the metalloprotease FtsH that the ATP-claimed in claim 1 that encodes relies on.
3. Nucleotide as claimed in claim 2, its sequence is SEQ ID NO:2.
4. a recombinant plasmid, is characterized in that, the metalloprotease FtsH that described expression of recombinant plasmid ATP-claimed in claim 1 relies on.
5. recombinant plasmid as claimed in claim 4, is characterized in that, the nucleotides sequence of the gene that described recombinant plasmid carries is classified SEQ ID NO:2 as.
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| CN111088202B (en) * | 2019-12-25 | 2022-01-04 | 南京工业大学 | Recombinant corynebacterium glutamicum for producing lysine through biological film-forming continuous fermentation and construction method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0801132A2 (en) * | 1996-03-14 | 1997-10-15 | Smithkline Beecham Plc | Staphylococcal FtsH protein |
| CN1328053A (en) * | 2000-06-14 | 2001-12-26 | 上海博德基因开发有限公司 | Polypeptide-human ftsH protein 16.83 and polynucleotide for coding it |
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2012
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0801132A2 (en) * | 1996-03-14 | 1997-10-15 | Smithkline Beecham Plc | Staphylococcal FtsH protein |
| CN1328053A (en) * | 2000-06-14 | 2001-12-26 | 上海博德基因开发有限公司 | Polypeptide-human ftsH protein 16.83 and polynucleotide for coding it |
Non-Patent Citations (8)
| Title |
|---|
| ATP-Dependent proteinases in bacteria;O. Hlavácek et al.;《Folia Microbiologica》;20020630;第47卷(第3期);203-212 * |
| Chen,Y. et al..GenBank: AEJ42239.1.《Genbank》.2011,1-2. |
| GenBank: ACV57263.1;Mavromatis,K. et al.;《Genbank》;20100617;1-2 * |
| GenBank: AEJ42239.1;Chen,Y. et al.;《Genbank》;20110920;1-2 * |
| Mavromatis,K. et al..GenBank: ACV57263.1.《Genbank》.2010,1-2. |
| O. Hlavácek et al..ATP-Dependent proteinases in bacteria.《Folia Microbiologica》.2002,第47卷(第3期),203-212. |
| 孙爱清 等.植物中的金属蛋白酶FtsH.《植物生理学通讯》.2006,第42卷(第1期),148-154. |
| 植物中的金属蛋白酶FtsH;孙爱清 等;《植物生理学通讯》;20060228;第42卷(第1期);148-154 * |
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