CN102425039A - Method for preparing water-soluble chitosan fiber membrane - Google Patents
Method for preparing water-soluble chitosan fiber membrane Download PDFInfo
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- CN102425039A CN102425039A CN2011102876488A CN201110287648A CN102425039A CN 102425039 A CN102425039 A CN 102425039A CN 2011102876488 A CN2011102876488 A CN 2011102876488A CN 201110287648 A CN201110287648 A CN 201110287648A CN 102425039 A CN102425039 A CN 102425039A
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000012528 membrane Substances 0.000 title abstract description 28
- 239000000835 fiber Substances 0.000 title abstract description 9
- 238000009987 spinning Methods 0.000 claims abstract description 68
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 57
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 40
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 24
- 239000002121 nanofiber Substances 0.000 claims abstract description 11
- 208000012886 Vertigo Diseases 0.000 claims description 80
- 239000000843 powder Substances 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000004745 nonwoven fabric Substances 0.000 claims description 3
- 239000003125 aqueous solvent Substances 0.000 claims description 2
- 239000003021 water soluble solvent Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 119
- 239000002904 solvent Substances 0.000 abstract description 3
- 239000012046 mixed solvent Substances 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 abstract 1
- 229910052782 aluminium Inorganic materials 0.000 abstract 1
- 239000011888 foil Substances 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 239000002085 irritant Substances 0.000 abstract 1
- 231100000021 irritant Toxicity 0.000 abstract 1
- 230000037314 wound repair Effects 0.000 description 18
- 239000003814 drug Substances 0.000 description 17
- 231100000252 nontoxic Toxicity 0.000 description 16
- 230000003000 nontoxic effect Effects 0.000 description 16
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 15
- 239000007864 aqueous solution Substances 0.000 description 15
- 150000001875 compounds Chemical class 0.000 description 15
- 229920002674 hyaluronan Polymers 0.000 description 15
- 229960003160 hyaluronic acid Drugs 0.000 description 15
- 238000003756 stirring Methods 0.000 description 15
- 239000000463 material Substances 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 230000005611 electricity Effects 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- 239000006184 cosolvent Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920005615 natural polymer Polymers 0.000 description 2
- 229920000867 polyelectrolyte Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- Nonwoven Fabrics (AREA)
Abstract
The invention relates to a method for preparing a water-soluble chitosan fiber membrane. Dissolving water-soluble chitosan in a mixed solvent of water and ethanol to prepare a solution, standing the solution for ultrasonic waves for 1 hour to completely separate out bubbles to obtain a water-soluble chitosan solution; adding the prepared water-soluble chitosan electrostatic spinning solution into an injection pump, adjusting proper spinning voltage, distance from a spinning nozzle to a collecting plate and flow of the spinning solution of the spinning nozzle, and performing electrostatic spinning on the aluminum foil within the temperature range of 10-40 ℃ to obtain the water-soluble chitosan nanofiber membrane. The invention adopts water-soluble chitosan as a raw material, avoids the use of an irritant solvent, increases the biocompatibility of the obtained nanofiber membrane, and expands the application range of the chitosan nanofiber membrane.
Description
Technical field
The present invention relates to a kind of method for preparing the water soluble chitosan tunica fibrosa.
Background technology
Shitosan [CS, chitosan, (1,4)-2-amino-2-deoxidation-β-D-glucose] is to take off a kind of linear macromolecule material that obtains after acetyl is handled by the natural polymer chitin, is a kind of natural biological macromolecular material with many advantageous properties.CS is owing to but characteristics such as its good antibiotic property and biocompatibility, inanimate object toxicity and Natural Degradation are widely used in biomedicine field.But CS can only be dissolved in the dilute acid soln of minority, can't be directly soluble in water, this big limitations the Application of Chitosan scope, people carry out chemical modification to amino or hydroxyl on the chitosan molecule chain and have obtained water soluble chitosan for this reason.Water soluble chitosan can directly be dissolved in the system of neutral and weakly acidic water environment, has greatly promoted CS as tissue engineering bracket material, drug carrier material and the application of wound repair material under the human body fluid environment.
The electricity spining technology is to utilize high-pressure electrostatic with polymer or have a kind of process technology that viscoelastic formulations prepared from solutions becomes the nanometer grade diameter fiber.Spinning solution receives the high-pressure electrostatic effect and has electric charge at spinning nozzle, and the mutual repulsion of surface charge makes liquid jet penetrate and obtain to stretch from spinning nozzle, is accompanied by the volatilization of solvent and solidifies, and finally on gatherer, obtains fiber.Initial electric spining technology is mainly used in preparation synthetic polymer nanofiber.Can a large amount of natural polymers be prepared into nanofiber through electrostatic spinning technique now.The electricity spinning fibre film is because its high-specific surface area, good bionic performance, carry at bioengineered tissue support, medicine release, there is higher using value aspect such as wound repair.CS is a kind of polycation natural polyelectrolyte, and strand is rigidity and owing to repels each other between the polyelectrolyte characteristic strand.Thereby be difficult to prepare into nano fibrous membrane owing to lacking necessary chain entanglement property through electrical spinning method for pure CS system.Through blending method with CS and synthetic high polymer such as polyethylene glycol oxide (PEO) or polyvinyl alcohol blend such as (PVA) after, prepare into nano fibrous membrane (Chinese patent CN1569254A) through electrical spinning method.Or through the cosolvent method regulate the spinning system parameter such as surface tension, electrical conductivity, viscosity and spinning technology parameter such as voltage, extruded velocity, put flight lead and leave etc. and to obtain CS nano fibrous membrane (Carbohydrate Polymers 77 (2009) 656-661; Macromol.Rapid Commun.2004,25,1600-1605).These methods are dissolved CS mostly in acid system, especially the cosolvent method must add concentration formic acid and high to reduce surface tension.Can there be small amount of residual in these acid in the CS tunica fibrosa of preparation, and influence the CS tunica fibrosa biocompatibility, increase its bio-toxicity, and then limited the range of application of CS.
The water-soluble CS that the present invention passes through to adopt modification through selecting suitable spinning process condition, has prepared good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane as raw material.This tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Summary of the invention
To the water soluble chitosan nano fibrous membrane of present preparation all in acid system electrostatic spinning obtain, the object of the present invention is to provide a kind of method for preparing the water soluble chitosan nano fibrous membrane.This method operating process mild condition, be easy to control, step is simple, with low cost.
A kind of method for preparing the water soluble chitosan nano fibrous membrane provided by the present invention may further comprise the steps:
(1) preparation of water soluble chitosan electricity spinning solution: in the water soluble chitosan powder is water-soluble, that ethanol the is formed mixed solvent; Be made into the solution that percentage by weight is 1～5wt%; Then solution is fully stirred; So that dissolving made bubble all separate out in 1 hour the solution left standstill ultrasonic wave and promptly obtains transparent water soluble chitosan solution fully.Described water soluble chitosan weight average molecular weight is 100,000～200,000, and the volume ratio of aqueous solvent, ethanol is 80/20～60/40.
(2) electrostatic spinning prepares the water soluble chitosan nanofiber: the water soluble chitosan electrostatic spinning solution of preparation in the step (1) is added syringe pump, and adjustment voltage is 15～30kV; Spinning head is to the distance 8～20cm of collecting board; The flow of spinning head spinning solution is 0.5～2.0mL/h; The spinning environment temperature is 10～40 ℃; Starting drive carries out the electrostatic spinning of water soluble chitosan, on collecting board, has obtained the water soluble chitosan nano fiber non-woven fabric, and distribution of fiber diameters is even, and fibre diameter is between 200nm～600nm.
Compared with prior art, the inventive method has the following advantages:
1) nanofiber that obtains of the inventive method, the tiny (200nm～600nm) of fibre diameter;
2) the inventive method is low for equipment requirements, and gathering-device, postprocessing working procedures are simple, have reduced cost;
3) the present invention adopts water-soluble CS system, does not contain the excitant solvent in the system, and gained nanometer fiber membrane biological compatibility is good.
Description of drawings
Fig. 1 is the nanofiber sem photograph by embodiment 1 preparation gained water soluble chitosan;
The specific embodiment
Embodiment 1
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 100,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 1wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is that the flow of 8cm spinning head spinning solution is 1mL/h to the distance of collecting board; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 2
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 1wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 3
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 200,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 1wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 4
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 5
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 5wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 6
1) compound concentration is the aqueous solution of the ethanol of 30wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 7
1) compound concentration is the aqueous solution of the ethanol of 40wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 8
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is that the flow of 8cm spinning head spinning solution is 0.5mL/h to the distance of collecting board; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 9
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 2mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 10
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 25kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 11
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 30kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 12
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 15cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 13
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 20cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 30 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 14
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is 8cm to the distance of collecting board; The flow of spinning head spinning solution is 1mL/h; The spinning environment temperature is 20 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Embodiment 15
1) compound concentration is the aqueous solution of the ethanol of 20wt%;
2) be that 150,000 water soluble chitosan powder are distributed in the step 1) solution with weight average molecular weight, be made into the solution that percentage by weight is 3wt%;
3) then with step 2) in solution fully stir so that fully the dissolving, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtains transparent hyaluronic acid solution;
4) solution in the step 3) is added syringe pump, adjustment voltage is 15kV; Spinning head is that the flow of 8cm spinning head spinning solution is 1mL/h to the distance of collecting board; The spinning environment temperature is 40 ℃;
5) starting drive carries out the electrostatic spinning of water soluble chitosan; Can on device, obtain good biocompatibility, nontoxic and biodegradable CS nano fibrous membrane on the collecting board, this tunica fibrosa all has higher using value at aspects such as organizational project, wound repair, medicament transport.
Claims (5)
1. method for preparing the water soluble chitosan nano fiber non-woven fabric is characterized in that may further comprise the steps:
(1) with in the water-soluble solvent of forming with ethanol of water soluble chitosan powder; Be made into the solution that percentage by weight is 1～5wt%; Then solution is fully stirred, so that dissolving fully, bubble is all separated out the solution left standstill ultrasonic wave and promptly obtain water soluble chitosan solution.
(2) the water soluble chitosan electrostatic spinning solution with preparation in the step (1) adds syringe pump, and the adjustment spinning voltage does; Spinning head is to the distance of collecting board; The flow of spinning head spinning solution does; The spinning environment temperature.Starting drive carries out the electrostatic spinning of water soluble chitosan, on collecting board, has obtained the water soluble chitosan nano fiber non-woven fabric;
2. according to the preparation method of claim 1, it is characterized in that the water soluble chitosan weight average molecular weight described in the step (1) is 100,000～200,000;
3. according to the preparation method of claim 1, the volume ratio that it is characterized in that aqueous solvent described in the step (1) and ethanol is 80/20～60/40, and the percentage by weight of water soluble chitosan solution is 1wt%～5wt%;
4. according to the preparation method of claim 1, it is characterized in that the voltage described in the step (2) is 15～30kV; Spinning head is 8～20cm to the distance of collecting board;
5. according to the preparation method of claim 1, it is characterized in that the spinning environment temperature described in the step (2) is 10～40 ℃.
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Cited By (8)
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|---|---|---|---|---|
| CN102677391A (en) * | 2012-05-07 | 2012-09-19 | 东华大学 | Preparation method of high-selectivity nano fiber membrane |
| CN103061037A (en) * | 2013-01-11 | 2013-04-24 | 东华大学 | Method for manufacturing polyaspartic acid nano-fiber mat by electrostatic spinning |
| CN105605339A (en) * | 2016-03-16 | 2016-05-25 | 刘志光 | High-strength antibacterial oxygen-resistant PP-RCT composite pipe |
| CN105926053A (en) * | 2016-06-08 | 2016-09-07 | 海南大学 | Electrostatic spinning method of chitosan |
| CN106757788A (en) * | 2016-12-23 | 2017-05-31 | 杭州安诺过滤器材有限公司 | A kind of filter for infusion filtering nano fibrous membrane and preparation method thereof |
| CN106963973A (en) * | 2017-03-17 | 2017-07-21 | 广州润虹医药科技有限公司 | A kind of chitosan combine dressing and preparation method thereof |
| CN111020745A (en) * | 2019-12-31 | 2020-04-17 | 泰州市榕兴医疗用品股份有限公司 | Preparation method of sodium alginate and chitosan composite nanofiber |
| CN113186649A (en) * | 2021-04-30 | 2021-07-30 | 河北宁纺集团有限责任公司 | Colorful antibacterial nanofiber membrane and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102677391A (en) * | 2012-05-07 | 2012-09-19 | 东华大学 | Preparation method of high-selectivity nano fiber membrane |
| CN103061037A (en) * | 2013-01-11 | 2013-04-24 | 东华大学 | Method for manufacturing polyaspartic acid nano-fiber mat by electrostatic spinning |
| CN105605339A (en) * | 2016-03-16 | 2016-05-25 | 刘志光 | High-strength antibacterial oxygen-resistant PP-RCT composite pipe |
| CN105926053A (en) * | 2016-06-08 | 2016-09-07 | 海南大学 | Electrostatic spinning method of chitosan |
| CN106757788A (en) * | 2016-12-23 | 2017-05-31 | 杭州安诺过滤器材有限公司 | A kind of filter for infusion filtering nano fibrous membrane and preparation method thereof |
| CN106757788B (en) * | 2016-12-23 | 2019-07-30 | 杭州安诺过滤器材有限公司 | A kind of filter for infusion filtering nano fibrous membrane and preparation method thereof |
| CN106963973A (en) * | 2017-03-17 | 2017-07-21 | 广州润虹医药科技有限公司 | A kind of chitosan combine dressing and preparation method thereof |
| CN111020745A (en) * | 2019-12-31 | 2020-04-17 | 泰州市榕兴医疗用品股份有限公司 | Preparation method of sodium alginate and chitosan composite nanofiber |
| CN113186649A (en) * | 2021-04-30 | 2021-07-30 | 河北宁纺集团有限责任公司 | Colorful antibacterial nanofiber membrane and preparation method and application thereof |
| CN113186649B (en) * | 2021-04-30 | 2022-11-08 | 河北宁纺集团有限责任公司 | Colorful antibacterial nanofiber membrane and preparation method and application thereof |
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