CN102334680A - Monascus cubilose and preparation method thereof - Google Patents
Monascus cubilose and preparation method thereof Download PDFInfo
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- CN102334680A CN102334680A CN2011102251458A CN201110225145A CN102334680A CN 102334680 A CN102334680 A CN 102334680A CN 2011102251458 A CN2011102251458 A CN 2011102251458A CN 201110225145 A CN201110225145 A CN 201110225145A CN 102334680 A CN102334680 A CN 102334680A
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Abstract
The invention discloses a monascus cubilose and a preparation method thereof. Monascus mother starter is inoculated into a culture medium, the pile is flattened, and sterile air is supplied for culture; the air volume per kilogram of materials is controlled at 4m<3> to 6m<3> per hour, the culture temperature is controlled at 30 DEG C to 32 DEG C, the moisture content of the materials is controlled at 50 to 60 percent by weight, culture takes 7 to 10 days, in the meantime, the monascus is turned and mashed, and thereby ripe monascus is obtained; a cubilose is soaked in sterile water or monascus spore suspension, the soaked cubilose is then buried into the ripe monascus, and the culture conditions are the same as in the previous step; the cubilose is taken out, washed with sterile water and aired, and thereby the monascus cubilose, which has the pharmacological effects of both the monascus and the cubilose, the monascus of which is uniformly distributed and the appearance and color of which are good, is obtained. The preparation method prepares the monascus cubilose on the basis of monascus preparation, and since the monascus has a certain bacteriostatic effect, the whole process cannot be easily polluted by foreign bacteria; since the solid-state fermentation technology is adopted, the monascus cubilose keeps the appearance integrity of the original cubilose, and after being washing and aired, the monascus cubilose does not have peculiar smell.
Description
Technical field
The present invention relates to a kind of bird's nest and preparation method thereof, particularly a kind of red colouring agent for food, also used as a Chinese medicine bird's nest and preparation method thereof belongs to biological technical field.
Background technology
Monascus (Mononscus) is typical small-sized thread saprophytic fungus, belongs to mycota (Eumycophyta), Ascomycota (Ascomycetes), Euascomycetes (Euascomycetes), plectomycete order (Eurotiales), red colouring agent for food, also used as a Chinese medicine Cordycepps (Monascaceae), monascus (Monas.cus).Red colouring agent for food, also used as a Chinese medicine is the culture of monascus; It is with a long history to adopt monascus to make red colouring agent for food, also used as a Chinese medicine; Since ancient times red colouring agent for food, also used as a Chinese medicine be used for wine brewing, make vinegar, brewage fermented bean curd, cured meat goods, food color and Chinese medicine etc., its effect is all on the books in the Exploitation of the Works of Nature and the Compendium of Material Medica of " the daily book on Chinese herbal medicine " in the Yuan Dynasty, the Ming Dynasty.Monascus can produce natural colouring matter with it and gain the name; In recent decades; People find that successively monascus can also produce multiple secondary metabolite with physiologically active, like Mo Na Kelin (Monacolin) compounds, GABA, acetylcholine, ergosterol etc.The discovery of these materials, confirmed red colouring agent for food, also used as a Chinese medicine have anticorrosion, reduce effects such as blood fat, hypotensive, hypoglycemic, tumor-inhibiting anticancer, raising body immunity.
The bird's nest nest nest that to be Apodidae (Apodidae) the esculent swift oozy saliva of multiple birds that belongs to (Collocalia) build up with bonding such as down, weeds, impurity such as warp removal down, weeds are the commodity bird's nest.Modern study shows that bird's nest not only can be used as additional protein, amino acid whose invigorant, and contains some activated proteins, has to suppress blood clotting, promote cell division, delay the brain tissue aging, improve effect such as immunity.Therefore, bird's nest is widely known medicine-food two-purpose high tonic, is usually used in tonifying lung yin-nourishing, tonifying middle-Jiao and Qi, moisturizes withered, the preventing phlegm from forming and stopping coughing in pool, cures mainly diseases such as tuberculosis, cough, spitting of blood, deficient, asthma due to excessive phlegm.
At present, not relevant for the report that adopts monascus that bird's nest is fermented.
Summary of the invention
The shortcoming that primary and foremost purpose of the present invention is to overcome prior art provides a kind of preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest with not enough.This preparation method is a process for solid state fermentation, monascus is grown on bird's nest and forms the red colouring agent for food, also used as a Chinese medicine bird's nest.
Another goal of the invention of the present invention is to provide the red colouring agent for food, also used as a Chinese medicine that obtains through above-mentioned preparation method bird's nest.This red colouring agent for food, also used as a Chinese medicine bird's nest not only outward appearance is good, and has the pharmaceutical component of monascus and bird's nest concurrently, and commodity value is high.
The object of the invention is realized through following technical proposals: a kind of preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest comprises following steps:
(1) preparation of culture medium: raw material after will pulverizing and water mixing, 121～125 ℃ of sterilizations obtain culture medium; Raw material comprises rice, soybean, wheat, corn, wheat bran or dregs of beans etc. for the nutriment of monascus growth can be provided;
(2) preparation of ripe red colouring agent for food, also used as a Chinese medicine: after treating culture medium cooling that step (1) prepares, it is bent in culture medium, to insert the monascus kind, mixes, and shakeouts the heap layer, feeds filtrated air and cultivates; Per kilogram material ventilation hourly is controlled to be 4～6m
3, cultivation temperature is controlled to be 30～32 ℃, material water ratio is controlled to be 50%～60% (mass percent), cultivated 7～10 days, during turn over bent with smash song, obtain ripe red colouring agent for food, also used as a Chinese medicine;
(3) preparation of red colouring agent for food, also used as a Chinese medicine bird's nest: soak into bird's nest with sterilized water or monascus spore suspension, the bird's nest after will soaking into is then imbedded in the ripe red colouring agent for food, also used as a Chinese medicine, feeds filtrated air and cultivates; Per kilogram material ventilation hourly is controlled to be 4～6m
3, cultivation temperature is controlled to be 30～32 ℃, material water ratio is controlled to be 50%～60% (mass percent), cultivated 7～10 days, during turn over bent with smash song, the monascus ruber filament can be grown on bird's nest surface and inner equably; Take out bird's nest, air-dry with the sterilized water washing, obtain the red colouring agent for food, also used as a Chinese medicine bird's nest that monascus and bird's nest are combined closely.
It is 60～80 purpose raw material powders that raw material after the pulverizing described in the step (1) is preferably particle diameter;
Raw material described in the step (1) is preferably by rice and dregs of beans and obtains by 2: 1 proportionings of mass ratio, or for wheat bran, soybean and rice obtain by 5: 3: 2 proportionings of mass ratio, or be that wheat, soybean and corn obtain by 1: 1: 1 proportioning of mass ratio;
1: 1.2～1.5 proportionings that raw material after the pulverizing described in the step (1) and described water are preferably pressed mass ratio;
The condition of the sterilization described in the step (1) is preferably 121～125 ℃ of sterilization 90～120min;
Monascus specie described in the step (2) derives from Chinese industrial microorganism fungus kind preservation administrative center, or derives from Chinese common micro-organisms culture presevation administrative center, like CICC5038, CGMCC3.991, but is not limited to said bacterial classification;
Monascus kind song described in the step (2) is preferably and presents red spore and gather, and spore count is 5 * 10
9More than individual/g (in butt);
Monascus kind Qu Youxuan described in the step (2) prepares through following steps: the monascus slant strains is shaken a bottle enlarged culture; Shaking bottle condition of culture is 30～32 ℃, 200～250rpm; Cultivated 7～10 days; Turn over song during this time, keeping the solid medium moisture is 50%～60% (mass percent);
The described bottle culture medium that shakes is for providing the nutriment of monascus growth; Comprise rice, soybean, wheat, corn, wheat bran, dregs of beans and brewer's wort etc.; Being preferably brewer's wort, rice and dregs of beans obtains by 3.6: 2: 1 proportionings of mass ratio; Or for brewer's wort, wheat bran and soybean obtain by 3.6: 2: 1 proportionings of mass ratio, or be brewer's wort, wheat bran, rice and soybean by mass ratio 4.5: 1: 1: 1 proportioning obtains;
The bent inoculum concentration of above-mentioned monascus kind is preferably mass percent 1%～10%;
Thickness after material described in the step (2) shakeouts is preferably 5～20cm;
Material described in the step (2) preferably places koji-making through heavy layer ventilation equipment system song;
Described in the step (2) turn over bent with smash song and carry out simultaneously, turn over song, smash and be preferably 12～24 hours bent blanking time;
Ripe red colouring agent for food, also used as a Chinese medicine described in the step (2) is that bent material is loose, and mycelia is healthy and strong, and red spore is grown thickly, and does not evenly have sandwichly, and spore count is at least 5 * 10
9Individual/g (in butt);
Bird's nest described in the step (3) is official swallow bird's nest commodity;
The time of the infiltration described in the step (3) is preferably 20～30min;
Washing with sterilized water described in the step (3) should be purpose with the solid impurity of cleaning red colouring agent for food, also used as a Chinese medicine bird's nest surface;
Air-dry temperature described in the step (3) is preferably 30～40 ℃;
Air-dry described in the step (3), should reduce to below 8% with moisture in the red colouring agent for food, also used as a Chinese medicine bird's nest is purpose;
A kind of red colouring agent for food, also used as a Chinese medicine bird's nest obtains through above-mentioned preparation method.
The present invention has following advantage and effect with respect to prior art:
(1) the present invention prepares at red colouring agent for food, also used as a Chinese medicine and prepares the red colouring agent for food, also used as a Chinese medicine bird's nest on the basis, because red colouring agent for food, also used as a Chinese medicine has certain bacteriostasis, thereby whole process is difficult for pollution microbes.
(2) the present invention adopts solid-state fermentation technology, and the red colouring agent for food, also used as a Chinese medicine bird's nest can keep the outward appearance integrality of original bird's nest, through the washing, air-dry after free from extraneous odour.
(3) the prepared red colouring agent for food, also used as a Chinese medicine bird's nest of the present invention is the body of combining closely of monascus thalline and bird's nest, has both the pharmacological effect of monascus and bird's nest, and monascus is evenly distributed, and appearance color is good.
Description of drawings
Fig. 1 is preparation method's schematic flow sheet of the present invention.
The specific embodiment
Below in conjunction with embodiment and accompanying drawing the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
The process of preparation red colouring agent for food, also used as a Chinese medicine bird's nest is as shown in Figure 1:
(1) preparation of solid-state fermentation culture medium
Rice, dregs of beans are pulverized respectively, sieved, obtain the powder between 60～80 orders.Take by weighing 2kg rice meal, 1kg bean cake powder, add 3.6kg water, mix thoroughly, place 121～125 ℃ of sterilization 90min, the cooling back is subsequent use.
(2) preparation of red colouring agent for food, also used as a Chinese medicine
The red colouring agent for food, also used as a Chinese medicine preparation flow is: monascus slant strains → kind of song shakes the ventilation of bottle cultivation → red colouring agent for food, also used as a Chinese medicine thick-layer and cultivates
Plant song and shake a bottle culture medium: brewer's wort (10 ° of Brix) 36g, rice meal (60～80 order) 20g, bean cake powder (60～80 order) 10g mixes thoroughly, and the 1000mL triangular flask of packing into places 121～125 ℃ of sterilization 90min, and the cooling back is subsequent use.
(the monascus slant strains (CICC5038 purchases in Chinese industrial microorganism fungus kind preservation administrative center) of 18mm * 180mm) all inserts kind of songs and shakes a bottle culture medium with 1 test tube; Mix thoroughly, place shaken cultivation on 30 ℃, the shaking table of 200r/min, whenever turn over kind of song 1 time at a distance from 24 h; Make it loose evenly, and suitably replenish sterilized water, make its moisture remain 50%～60% (mass percent); Cultivated 7 days, it is bent promptly to get the monascus kind.The red spore of this monascus kind Qu Chengxian gathers, and spore count is 5.5 * 10
9Individual/g (in butt).
With the solid-state fermentation culture medium that the bent all access steps (1) of above-mentioned monascus kind prepare, inoculum concentration is 1% (mass percent), mixes thoroughly; The stand is gone in the koji-making through heavy layer ventilation equipment; Material thickness is about 5cm, feeds filtrated air then and cultivates, and per hour throughput is 31.7m
3, cultivation temperature is controlled to be 30～32 ℃, and it is 50%～60% (mass percent) that incubation keeps moisture content of material, whenever turns over song, smashes bent 1 time at a distance from 24 h, makes it loose even, cultivates 9 days, promptly gets ripe red colouring agent for food, also used as a Chinese medicine.This maturation red colouring agent for food, also used as a Chinese medicine presents that bent material is loose, and mycelia is healthy and strong, and red spore is grown thickly, and does not evenly have sandwichly, and spore count is 5.2 * 10
9Individual/g (in butt).
(3) fermentation of red colouring agent for food, also used as a Chinese medicine bird's nest
Select official swallow bird's nest commodity (A level swallow small cup for use; Originate from Malaysia, white), its hydrolysising amino acid content is 48.8% (mass percent); Sialic acid content is 10.9% (mass percent), does not detect Monacolin K (Monacolin K), GABA, ergosterol.
Soak into commodity bird's nest 20min with sterilized water, then bird's nest is imbedded in the heap layer of ripe red colouring agent for food, also used as a Chinese medicine, continue to ventilate and cultivate, per hour throughput is 26.7m
3, cultivation temperature is controlled to be 30～32 ℃, and it is 50%～60% (mass percent) that incubation keeps moisture content of material; Whenever turn over song, smash bent 1 time at a distance from 24 h, make it loose evenly, cultivate after 10 days; The monascus ruber filament is evenly grown on bird's nest surface and inner, takes out bird's nest then, cleans bird's nest surface solid impurity with sterilized water; Place carry out under 30 ℃ of conditions air-dry to water content be 7.9%, can obtain red red colouring agent for food, also used as a Chinese medicine bird's nest.Monascus is evenly distributed in the red colouring agent for food, also used as a Chinese medicine bird's nest, and monascus and bird's nest are combined closely, and does not break away from through washing; Red colouring agent for food, also used as a Chinese medicine bird's nest free from extraneous odour, storage requirement and time limit and former bird's nest are as good as.
Through detecting; The hydrolysising amino acid content of gained red colouring agent for food, also used as a Chinese medicine bird's nest is 48.2% (mass percent), and sialic acid content is 10.8% (mass percent), and Monacolin K (Monacolin K) content is 4.12 μ g/g; Alpha-aminobutyric acid content is 1.63 μ g/g, and ergosterol content is 0.59 μ g/g.
It is thus clear that, using monascus fermentation bird's nest, monascus does not consume the active component of bird's nest.The red colouring agent for food, also used as a Chinese medicine bird's nest of present embodiment preparation has had both the functional components of bird's nest and monascus.
Embodiment 2
The process of preparation red colouring agent for food, also used as a Chinese medicine bird's nest is as shown in Figure 1:
(1) preparation of solid-state fermentation culture medium
Wheat bran, soybean, rice are pulverized respectively, sieved, obtain the powder between 60～80 orders, take by weighing 6kg wheat bran, 3.5kg soy meal, the 2.5kg rice meal adds 14.4kg water, mixes thoroughly, places 121～125 ℃ of sterilization 120min, and the cooling back is subsequent use.
(2) preparation of red colouring agent for food, also used as a Chinese medicine
The red colouring agent for food, also used as a Chinese medicine preparation flow is: monascus slant strains → kind of song shakes the ventilation of bottle cultivation → red colouring agent for food, also used as a Chinese medicine thick-layer and cultivates
Plant song and shake a bottle culture medium: brewer's wort (12 ° of Brix) 1440g, wheat bran (60～80 order) 800g, soy meal (60～80 order) 400g mixes thoroughly, divides 20 the 1000mL triangular flasks of packing into, places 121～125 ℃ of sterilization 60min, and the cooling back is subsequent use.
(the monascus slant strains of 18mm * 180mm) (CGMCC3.991 purchases in Chinese common micro-organisms culture presevation administrative center) inserts 20 kind songs respectively and shakes a bottle culture medium, mixes thoroughly respectively with 20 test tubes; Place shaken cultivation on 30 ℃, the shaking table of 200r/min, whenever turn over kind of song 1 time, make it loose evenly at a distance from 12h; And suitably additional sterilized water; Make its moisture remain 50%～60% (mass percent), cultivated 7 days, it is bent promptly to get the monascus kind.The red spore of this monascus kind Qu Chengxian gathers, and spore count is 5.7 * 10
9Individual/g (in butt).
With the bent solid-state fermentation culture medium that all inserts of above-mentioned 20 bottles of monascus kinds, inoculum concentration is 10% (mass percent), mixes thoroughly, and the stand is gone in the koji-making through heavy layer ventilation equipment, and material thickness is about 20cm, feeds filtrated air then and cultivates, and per hour throughput is 174.2m
3, cultivation temperature is controlled to be 30～32 ℃, and it is 50%～60% (mass percent) that incubation keeps moisture content of material, and every separated 12h turns over song, smashes bent 1 time, makes it loose even, cultivates 10 days, promptly gets ripe red colouring agent for food, also used as a Chinese medicine.This maturation red colouring agent for food, also used as a Chinese medicine presents that bent material is loose, and mycelia is healthy and strong, and red spore is grown thickly, and does not evenly have sandwichly, and spore count is 5.4 * 10
9Individual/g (in butt).
(3) fermentation of red colouring agent for food, also used as a Chinese medicine bird's nest
Select official swallow bird's nest commodity (A level swallow small cup for use; Originate from Indonesia, white), its hydrolysising amino acid content is 49.1% (mass percent); Sialic acid content is 10.6% (mass percent), does not detect Monacolin K (Monacolin K), GABA, ergosterol.
(spore count is 4 * 10 with the monascus spore suspension
8Individual/as mL) to soak into commodity bird's nest 30min, then bird's nest is imbedded in the heap layer of ripe red colouring agent for food, also used as a Chinese medicine, continue to ventilate and cultivate, per hour throughput is 174.2m
3, cultivation temperature is controlled to be 30～32 ℃, and it is 50%～60% (mass percent) that incubation keeps moisture content of material; Every separated 12h turns over song, smashes bent 1 time, makes it loose evenly, cultivates after 7 days; The monascus ruber filament is evenly grown on bird's nest surface and inner, takes out bird's nest then, cleans bird's nest surface solid impurity with sterilized water; Place carry out under 40 ℃ of conditions air-dry to water content be 7.2%, can obtain red red colouring agent for food, also used as a Chinese medicine bird's nest.Monascus is evenly distributed in the red colouring agent for food, also used as a Chinese medicine bird's nest, and monascus and bird's nest are combined closely, and does not break away from through washing; Red colouring agent for food, also used as a Chinese medicine bird's nest free from extraneous odour, storage requirement and time limit and former bird's nest are as good as.Through detecting; The hydrolysising amino acid content of gained red colouring agent for food, also used as a Chinese medicine bird's nest is 48.7% (mass percent), and sialic acid content is 10.4% (mass percent), and Monacolin K (Monacolin K) content is 3.75 μ g/g; Alpha-aminobutyric acid content is 1.52 μ g/g, and ergosterol content is 0.49 μ g/g.
It is thus clear that, using monascus fermentation bird's nest, monascus does not consume the active component of bird's nest.The red colouring agent for food, also used as a Chinese medicine bird's nest of present embodiment preparation has had both the functional components of bird's nest and monascus.
Embodiment 3
The process of preparation red colouring agent for food, also used as a Chinese medicine bird's nest is as shown in Figure 1:
(1) preparation of solid-state fermentation culture medium
Wheat, soybean, corn are pulverized respectively, sieved, obtain the powder between 60～80 orders, take by weighing 2kg wheat flour, 2kg soy meal, the 2kg corn flour adds 9kg water, mixes thoroughly, places 121～125 ℃ of sterilization 100min, and the cooling back is subsequent use.
(2) preparation of red colouring agent for food, also used as a Chinese medicine
The red colouring agent for food, also used as a Chinese medicine preparation flow is: monascus slant strains → kind of song shakes the ventilation of bottle cultivation → red colouring agent for food, also used as a Chinese medicine thick-layer and cultivates
Plant song and shake a bottle culture medium: brewer's wort (10 ° of Brix) 720g, wheat bran (60～80 order) 160g, soy meal (60～80 order) 160g; Rice meal (60～80 order) 160g mixes thoroughly, divides 16 the 1000mL triangular flasks of packing into; Place 121～125 ℃ of sterilization 120min, the cooling back is subsequent use.
(the monascus slant strains of 18mm * 180mm) (CGMCC3.991 purchases in Chinese common micro-organisms culture presevation administrative center) inserts 16 kind songs respectively and shakes a bottle culture medium, mixes thoroughly respectively with 16 test tubes; Place shaken cultivation on 30 ℃, the shaking table of 200r/min, whenever turn over kind of song 1 time, make it loose evenly at a distance from 16 h; And suitably additional sterilized water; Make its moisture remain 550%～60% (mass percent), cultivated 7 days, it is bent promptly to get the monascus kind.The red spore of this monascus kind Qu Chengxian gathers, and spore count is 5.3 * 10
9Individual/g (in butt).
With the bent solid-state fermentation culture medium that all inserts of above-mentioned 16 bottles of monascus kinds, inoculum concentration is 8% (mass percent), mixes thoroughly, and the stand is gone in the koji-making through heavy layer ventilation equipment, and material thickness is about 12cm, feeds filtrated air then and cultivates, and per hour throughput is 90m
3, cultivation temperature is controlled to be 30～32 ℃, and it is 50%～60% (mass percent) that incubation keeps moisture content of material, and every separated 16h turns over song, smashes bent 1 time, makes it loose even, cultivates 7 days, promptly gets ripe red colouring agent for food, also used as a Chinese medicine.This maturation red colouring agent for food, also used as a Chinese medicine presents that bent material is loose, and mycelia is healthy and strong, and red spore is grown thickly, and does not evenly have sandwichly, and spore count is 5.1 * 10
9Individual/g (in butt).
(3) fermentation of red colouring agent for food, also used as a Chinese medicine bird's nest
Select official swallow bird's nest commodity (A level swallow small cup for use; Originate from Malaysia, white), its hydrolysising amino acid content is 47.4% (mass percent); Sialic acid content is 10.5% (mass percent), does not detect Monacolin K (Monacolin K), GABA, ergosterol.
(spore count is 1 * 10 with the monascus spore suspension
8Individual/as mL) to soak into commodity bird's nest 25min, then bird's nest is imbedded in the heap layer of ripe red colouring agent for food, also used as a Chinese medicine, continue to ventilate and cultivate, per hour throughput is 90m
3, cultivation temperature is controlled to be 30～32 ℃, and it is 50%～60% (mass percent) that incubation keeps moisture content of material; Every separated 16h turns over song, smashes bent 1 time, makes it loose evenly, cultivates after 8 days; The monascus ruber filament is evenly grown on bird's nest surface and inner, takes out bird's nest then, cleans bird's nest surface solid impurity with sterilized water; Place carry out under 36 ℃ of conditions air-dry to water content be 7.5%, can obtain red red colouring agent for food, also used as a Chinese medicine bird's nest.Monascus is evenly distributed in the red colouring agent for food, also used as a Chinese medicine bird's nest, and monascus and bird's nest are combined closely, and does not break away from through washing; Red colouring agent for food, also used as a Chinese medicine bird's nest free from extraneous odour, storage requirement and time limit and former bird's nest are as good as.Through detecting; The hydrolysising amino acid content of gained red colouring agent for food, also used as a Chinese medicine bird's nest is 46.8% (mass percent); Sialic acid content is 10.2% (mass percent), and Monacolin K (Monacolin K) content is 3.66 μ g/g, and alpha-aminobutyric acid content is 1.61 μ g/g; Ergosterol content is 0.45 μ g/g, has had both the functional components of bird's nest and monascus.
It is thus clear that, using monascus fermentation bird's nest, monascus does not consume the active component of bird's nest.The red colouring agent for food, also used as a Chinese medicine bird's nest of present embodiment preparation has had both the functional components of bird's nest and monascus.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. the preparation method of a red colouring agent for food, also used as a Chinese medicine bird's nest is characterized in that comprising following steps:
(1) preparation of culture medium: raw material after will pulverizing and water mixing, 121～125 ℃ of sterilizations obtain culture medium; Raw material is for providing the nutriment of monascus growth;
(2) preparation of ripe red colouring agent for food, also used as a Chinese medicine: after treating culture medium cooling that step (1) prepares, it is bent in culture medium, to insert the monascus kind, mixes, and shakeouts the heap layer, feeds filtrated air and cultivates; Per kilogram material ventilation hourly is controlled to be 4～6m
3, cultivation temperature is controlled to be 30～32 ℃, material water ratio is controlled to be mass percent 50%～60%, cultivated 7～10 days, during turn over bent with smash song, obtain ripe red colouring agent for food, also used as a Chinese medicine;
(3) preparation of red colouring agent for food, also used as a Chinese medicine bird's nest: soak into bird's nest with sterilized water or monascus spore suspension, the bird's nest after will soaking into is then imbedded in the ripe red colouring agent for food, also used as a Chinese medicine, feeds filtrated air and cultivates; Per kilogram material ventilation hourly is controlled to be 4～6m
3, cultivation temperature is controlled to be 30～32 ℃, material water ratio is controlled to be mass percent 50%～60%, cultivated 7～10 days, during turn over bent with smash song; Take out bird's nest, air-dry with the sterilized water washing, obtain the red colouring agent for food, also used as a Chinese medicine bird's nest.
2. the preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest according to claim 1 is characterized in that: the raw material after the pulverizing described in the step (1) is that particle diameter is 60～80 purpose raw material powders;
Raw material described in the step (1) is at least a in rice, soybean, wheat, corn, wheat bran or the dregs of beans;
Raw material after the pulverizing described in the step (1) and described water carry out proportioning by mass ratio 1: 1.2～1.5;
The condition of the sterilization described in the step (1) is 121～125 ℃ of sterilization 90～120min.
3. the preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest according to claim 2; It is characterized in that: the raw material described in the step (1) is for to be obtained by 2: 1 proportionings of mass ratio by rice and dregs of beans; Or for wheat bran, soybean and rice obtain by 5: 3: 2 proportionings of mass ratio, or be that wheat, soybean and corn obtain by 1: 1: 1 proportioning of mass ratio.
4. the preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest according to claim 1 is characterized in that: the monascus described in the step (2) is CICC5038 or CGMCC3.991.
5. the preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest according to claim 1 is characterized in that: the monascus kind described in the step (2) is bent to be that spore count is at least 5 * 10 in butt
9The monascus kind of individual/g is bent;
Thickness after material described in the step (2) shakeouts is 5～20cm;
Material described in the step (2) places koji-making through heavy layer ventilation equipment system song;
Described in the step (2) turn over bent with smash song and carry out simultaneously, turning over song, smashing bent blanking time is 12～24 hours.
6. the preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest according to claim 5; It is characterized in that: the monascus kind triton described in the step (2) is crossed following steps and is prepared: the monascus slant strains is shaken a bottle enlarged culture; Shaking bottle condition of culture is 30～32 ℃, 200～250rpm; Cultivated 7～10 days, during turn over song, keeping the solid medium moisture is mass percent 50%～60%;
The bent inoculum concentration of described monascus kind is a mass percent 1%～10%.
7. the preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest according to claim 6 is characterized in that: the described culture medium that shakes bottle enlarged culture is rice, soybean, wheat, corn, wheat bran, dregs of beans or brewer's wort.
8. the preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest according to claim 7; It is characterized in that: the described culture medium that shakes bottle enlarged culture is that brewer's wort, rice and dregs of beans obtain by 3.6: 2: 1 proportionings of mass ratio; Or for brewer's wort, wheat bran and soybean obtain by 3.6: 2: 1 proportionings of mass ratio, or be brewer's wort, wheat bran, rice and soybean by mass ratio 4.5: 1: 1: 1 proportioning obtains.
9. the preparation method of red colouring agent for food, also used as a Chinese medicine bird's nest according to claim 1 is characterized in that:
The time of the infiltration described in the step (3) is 20～30min;
Air-dry temperature described in the step (3) is 30～40 ℃;
Air-dry degree described in the step (3) is that moisture is reduced to below 8% in the red colouring agent for food, also used as a Chinese medicine bird's nest.
10. red colouring agent for food, also used as a Chinese medicine bird's nest, the preparation method through each described red colouring agent for food, also used as a Chinese medicine bird's nest of claim 1～9 obtains.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103284060A (en) * | 2013-06-28 | 2013-09-11 | 安徽燕之坊食品有限公司 | Coarse cereal food containing monascus bacteria and fermented glutinous rice yeasts and preparation method thereof |
| CN107334100A (en) * | 2017-06-29 | 2017-11-10 | 张萌 | Bird's nest composite enzyme and preparation method thereof |
| CN107405365A (en) * | 2015-02-03 | 2017-11-28 | 森田敦子 | The preparation method of the material, component and the material that are made using esculent swift bird's nest as raw material |
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| CN101209096A (en) * | 2006-12-31 | 2008-07-02 | 中国科学院过程工程研究所 | Edible porridge and preparation method thereof |
| JP2009234980A (en) * | 2008-03-27 | 2009-10-15 | Japan Natural Laboratory Co Ltd | Cosmetic composition incorporating cell growth factor blended with neuraminic acids and placenta |
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| CN101209096A (en) * | 2006-12-31 | 2008-07-02 | 中国科学院过程工程研究所 | Edible porridge and preparation method thereof |
| JP2009234980A (en) * | 2008-03-27 | 2009-10-15 | Japan Natural Laboratory Co Ltd | Cosmetic composition incorporating cell growth factor blended with neuraminic acids and placenta |
Non-Patent Citations (1)
| Title |
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| 《湖北农学院学报》 20000531 陈义光 等 红曲及红曲霉的研究与应用 第188-191页 1-10 第20卷, 第02期 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103284060A (en) * | 2013-06-28 | 2013-09-11 | 安徽燕之坊食品有限公司 | Coarse cereal food containing monascus bacteria and fermented glutinous rice yeasts and preparation method thereof |
| CN103284060B (en) * | 2013-06-28 | 2014-05-07 | 安徽燕之坊食品有限公司 | Coarse cereal food containing monascus bacteria and fermented glutinous rice yeasts and preparation method thereof |
| CN107405365A (en) * | 2015-02-03 | 2017-11-28 | 森田敦子 | The preparation method of the material, component and the material that are made using esculent swift bird's nest as raw material |
| CN107405365B (en) * | 2015-02-03 | 2021-06-01 | 森田敦子 | Material prepared from swiftlet nest as raw material, components and preparation method of material |
| CN107334100A (en) * | 2017-06-29 | 2017-11-10 | 张萌 | Bird's nest composite enzyme and preparation method thereof |
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