CN102286573A - Method for hydrolyzing dextranin in syrup with enzyme - Google Patents
Method for hydrolyzing dextranin in syrup with enzyme Download PDFInfo
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- CN102286573A CN102286573A CN201110261294XA CN201110261294A CN102286573A CN 102286573 A CN102286573 A CN 102286573A CN 201110261294X A CN201110261294X A CN 201110261294XA CN 201110261294 A CN201110261294 A CN 201110261294A CN 102286573 A CN102286573 A CN 102286573A
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- Prior art keywords
- syrup
- dextran
- add
- dextranase
- 50ppm
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- 238000000034 method Methods 0.000 title claims abstract description 66
- 239000006188 syrup Substances 0.000 title claims abstract description 62
- 235000020357 syrup Nutrition 0.000 title claims abstract description 62
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 5
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 5
- 230000003301 hydrolyzing effect Effects 0.000 title abstract 2
- 108010001682 Dextranase Proteins 0.000 claims abstract description 29
- 229930006000 Sucrose Natural products 0.000 claims abstract description 15
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 15
- 229960004793 sucrose Drugs 0.000 claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 claims abstract description 13
- 229920002307 Dextran Polymers 0.000 claims description 61
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 34
- 230000007062 hydrolysis Effects 0.000 claims description 18
- 238000006460 hydrolysis reaction Methods 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 15
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 238000005352 clarification Methods 0.000 claims description 12
- 238000005086 pumping Methods 0.000 claims description 12
- 230000035484 reaction time Effects 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 10
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 7
- 240000000111 Saccharum officinarum Species 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000002002 slurry Substances 0.000 claims description 7
- 238000001704 evaporation Methods 0.000 claims description 6
- 230000008020 evaporation Effects 0.000 claims description 6
- 238000003801 milling Methods 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 239000002253 acid Substances 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 abstract 1
- 239000005720 sucrose Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 241000192130 Leuconostoc mesenteroides Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- CZMRCDWAGMRECN-UHFFFAOYSA-N Rohrzucker Natural products OCC1OC(CO)(OC2OC(CO)C(O)C(O)C2O)C(O)C1O CZMRCDWAGMRECN-UHFFFAOYSA-N 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- -1 small molecules carbohydrate Chemical class 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000009923 sugaring Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Landscapes
- Jellies, Jams, And Syrups (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for hydrolyzing the dextranin in syrup with enzyme. In the process of cane sugar manufacture by the sulfurous acid method, the method comprises the step: adding dextranase to process the syrup, the amount of the added dextranase depends on the content of the dextranin in the syrup, and is determined to be 10ppm to 50ppm of the weight of the syrup in the method, and the total usage of the dextranase is not larger than 100ppm of the total weight of various processed syrups. By using the method, the dextranin in syrup can be effectively hydrolyzed, the quality of syrup can be improved, the content of acid flocculates in products can be reduced and the product quality and the recovery rate can be increased.
Description
Technical field
The present invention relates to reduce in the sugar manufacturing process method of dextran content, specifically is a kind of method with dextran in the enzymic hydrolysis syrup.
Technical background
Microorganism mainly is because certain micro-organisms excretory invertase makes sucrose inversion to the harm of sugaring process, causes the sucrose loss, and wherein the most serious is the microorganism of Leuconostoc mesenteroides one class, consumes sucrose and generates dextran.The formation of dextran, approximately 7 tons of sucrose of per 10000 tons of sugarcane Direct Loss.Dextran is not only the main component of the acid throw out of white sugar, it is present in the sugarcane juice, also can obscure the pol reading, make false rising of purity of syrup, influence the production analysis management, and increase the viscosity of liquid glucose, sugar and purging operation are boiled in influence, residual sucrose amount increases in the molasses, and sugar producing rate descends, and these indirect losses are 3～5 times that sucrose inversion becomes the dextran loss.
Dextran can not be removed with the purification techniques of routine such as active carbon filtration and ion exchange resin, even ultra-filtration technique also can't be removed fully, the most effective at present removal method adds dextranase exactly, dextran is resolved into the small molecules carbohydrate, thereby reduce the influence of dextran production process and quality product.
Summary of the invention
The purpose of this invention is to provide a kind of method with dextran in the enzymic hydrolysis syrup, this method is with dextranase dextran in the syrup to be resolved into the small molecules carbohydrate, reduces dextran content, thereby reduces its influence to production process and quality product.
In sugar refinery produced, Leuconostoc mesenteroides consumed the sucrose fermentation and produces dextran.Dextranase can be α-D-1 with main chain, and the dextran hydrolysis of 6 key straight chain types again can be with α-D-1,3 keys or α-D-1, the dextran hydrolysis of 4 key branched chain types.
The technical scheme that the present invention solves the problems of the technologies described above is:
1. method with dextran in the enzymic hydrolysis syrup, this method is in cane sugar manufacture sulfitation process craft process, add dextran in the enzymic hydrolysis syrup, to reduce the content of dextran in the syrup, described enzyme is a dextranase, its addition is the 10ppm～50ppm of syrup weight, and its total addition level is not more than the 100ppm of processed all kinds of syrup gross weights.
2. method with dextran in the enzymic hydrolysis syrup, described syrup is the mixing juice that cane milling workshop section comes out, in the mixing juice of milling shop, add dextranase, its consumption is decided according to dextran content in the mixing juice, add-on is 10ppm～50ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times from be added to stove drying and before, totally 20 minutes, the dextran rate of removing was 45%, and filtration velocity and settling velocity have all improved about 40%, the clear juice clarification that obtains is bright, and turbidity reduces by 60%.
3. method with dextran in the enzymic hydrolysis syrup, described syrup is the clear juice that comes out from clarification workshop section, add dextranase in the clear juice after the clarification of sugarcane sulfurous method, its addition is decided according to dextran content in the clear juice, and add-on is 10ppm～50ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times finishes from being added to evaporative process, and totally 60 minutes, the dextran rate of removing was 23%.
4. method with dextran in the enzymic hydrolysis syrup, described syrup is the raw sugar slurry that comes out from evaporation section, in the raw sugar slurry that evaporation section comes out, add dextranase, its addition is decided according to dextran content in the syrup, and add-on is 10ppm～50ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times from be added to enter boiling pan before, totally 30 minutes, the dextran rate of removing was 15%.
Above-mentioned syrup refers to any two or three in mixing juice, clear juice, the syrup, add the dextranase in the syrup to amount be the 10ppm～50ppm of all kinds of syrup weight, its total addition level is not more than the 100ppm of processed all kinds of syrup gross weights.
The present invention has following effect in sugar manufacturing process:
1. improve syrup filtration velocity and settling velocity.Add crude enzyme liquid in mixing juice, carry out clarifying treatment with conventional sulfitation process craft, filtration velocity and settling velocity have all improved more than 40%.
2. reduce dextran content.Add dextranase and handle syrup, the rate of removing of dextran reaches 15～45%, has reduced the influence of dextran to the optically-active reading, and the adding of dextranase does not have influence to sucrose.
3. add dextranase in mixing juice, carry out clarifying treatment with conventional sulfitation process craft, the clear juice clarification that obtains is bright, and turbidity reduces by 60%.
4. technology is simple, need not to improve original technology, consumption seldom, cost is low, the efficient height can effectively improve the syrup quality, improves product hierarchy and recovery, and reduces the consumption of other finings (lime, sulphur, phosphoric acid), remarkable in economical benefits.
Embodiment
A kind of with dextran enzymic hydrolysis dextran, thereby improve the syrup method for quality, this method is in the clarification process of sugarcane or beet sugar manufacture process, dextranase is added in the syrup, dextran in the hydrolysis syrup, reduce dextran content, thereby reduce its influence production process and quality product.
Below in conjunction with embodiment content of the present invention is described further.
Embodiment 1
In the mixing juice of milling shop, add dextranase, its consumption is decided according to dextran content in the mixing juice, add-on is 10ppm, need not increase whipping appts, mixes in the pumping process and reaction, reaction times from be added to stove drying and before, totally 20 minutes, the dextran rate of removing was 45%, and filtration velocity and settling velocity have all improved about 40%, the clear juice clarification that obtains is bright, and turbidity reduces by 60%.
Embodiment 2
In the mixing juice of milling shop, add dextranase, its consumption is decided according to dextran content in the mixing juice, add-on is 50ppm, need not increase whipping appts, mixes in the pumping process and reaction, reaction times from be added to stove drying and before, totally 20 minutes, the dextran rate of removing was 60%, and filtration velocity and settling velocity have all improved about 50%, the clear juice clarification that obtains is bright, and turbidity reduces by 70%.
Embodiment 3
Add dextranase in the clear juice after the clarification of sugarcane sulfurous method, its consumption is decided according to dextran content in the clear juice, add-on is 10ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times finishes from being added to evaporative process, and totally 60 minutes, the dextran rate of removing was 23%.
Embodiment 4
Add dextranase in the clear juice after the clarification of sugarcane sulfurous method, its consumption is decided according to dextran content in the clear juice, add-on is 50ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times finishes from being added to evaporative process, and totally 60 minutes, the dextran rate of removing was 35%.
Embodiment 5
In the raw sugar slurry that evaporation section comes out, add dextranase, its consumption is decided according to dextran content in the syrup, add-on is 10ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times from be added to enter boiling pan before, totally 30 minutes, the dextran rate of removing was 15%.
Embodiment 6
In the raw sugar slurry that evaporation section comes out, add dextranase, its consumption is decided according to dextran content in the syrup, add-on is 50ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times from be added to enter boiling pan before, totally 30 minutes, the dextran rate of removing was 20%.
A kind of conversion as the embodiment of the invention, the syrup that also can be used for beet-sugar factory, and as required, add dextranase in mixing juice, clear juice, the raw sugar slurry any two or three respectively, consumption is decided according to the height of dextran content in the syrup, the dextranase amount of being added in the syrup is the 10ppm～50ppm of all kinds of syrup weight, and its total consumption is not more than the 100ppm of processed all kinds of syrup gross weights.
The embodiment of the invention is that dextranase can directly add in the corresponding syrup.
Claims (5)
1. method with dextran in the enzymic hydrolysis syrup, it is characterized in that: this method is in cane sugar manufacture sulfitation process craft process, add dextran in the enzymic hydrolysis syrup, to reduce the content of dextran in the syrup, described enzyme is a dextranase, its addition is the 10ppm～50ppm of syrup weight, and its total addition level is not more than the 100ppm of processed all kinds of syrup gross weights.
2. method with dextran in the enzymic hydrolysis syrup, it is characterized in that: this method is in cane sugar manufacture sulfitation process craft process, described syrup is the mixing juice that cane milling workshop section comes out, in the mixing juice of milling shop, add dextranase, its consumption is decided according to dextran content in the mixing juice, add-on is 10ppm～50ppm, need not increase whipping appts, in the pumping process, mix and reaction, the reaction times from be added to stove drying and before, totally 20 minutes, the dextran rate of removing is 45%, filtration velocity and settling velocity have all improved about 40%, and the clear juice clarification that obtains is bright, and turbidity reduces by 60%.
3. method with dextran in the enzymic hydrolysis syrup, it is characterized in that: this method is in cane sugar manufacture sulfitation process craft process, described syrup is the clear juice that comes out from clarification workshop section, add dextranase in the clear juice after the clarification of sugarcane sulfurous method, its addition is decided according to dextran content in the clear juice, add-on is 10ppm～50ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times finishes from being added to evaporative process, totally 60 minutes, the dextran rate of removing was 23%.
4. method with dextran in the enzymic hydrolysis syrup, it is characterized in that: this method is in cane sugar manufacture sulfitation process craft process, described syrup is the raw sugar slurry that comes out from evaporation section, in the raw sugar slurry that evaporation section comes out, add dextranase, its addition is decided according to dextran content in the syrup, add-on is 10ppm～50ppm, need not increase whipping appts, in the pumping process, mix and reaction, reaction times from be added to enter boiling pan before, totally 30 minutes, the dextran rate of removing was 15%.
5. according to claim 2 or claim 3 or the described a kind of method of claim 4 with dextran in the enzymic hydrolysis syrup, it is characterized in that: described syrup refers to any two or three in mixing juice, clear juice, the syrup, add the dextranase in the syrup to amount be the 10ppm～50ppm of all kinds of syrup weight, its total addition level is not more than the 100ppm of processed all kinds of syrup gross weights.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110261294XA CN102286573A (en) | 2011-09-06 | 2011-09-06 | Method for hydrolyzing dextranin in syrup with enzyme |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110261294XA CN102286573A (en) | 2011-09-06 | 2011-09-06 | Method for hydrolyzing dextranin in syrup with enzyme |
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| CN201110261294XA Pending CN102286573A (en) | 2011-09-06 | 2011-09-06 | Method for hydrolyzing dextranin in syrup with enzyme |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357426A (en) * | 2014-10-13 | 2015-02-18 | 华南理工大学 | Compound enzyme for cane molasses fermentation as well as preparation method and application thereof |
-
2011
- 2011-09-06 CN CN201110261294XA patent/CN102286573A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| 《2011年中国崇左蔗糖业发展大会》 20110805 陆海勤等 alpha-葡聚糖酶在甘蔗制糖中的应用研究进展 3-122-3-127 1-5 , * |
| 《2011年中国崇左蔗糖业发展大会》 20110805 麻少莹等 alpha-葡聚糖酶发酵工艺与酶活影响因素研究 4-31－4-38 1-5 , * |
| 梁达奉等: "葡聚糖对制糖工业的影响及对策(下)", 《甘蔗糖业》 * |
| 陆海勤等: "α-葡聚糖酶在甘蔗制糖中的应用研究进展", 《2011年中国崇左蔗糖业发展大会》 * |
| 麻少莹等: "α-葡聚糖酶发酵工艺与酶活影响因素研究", 《2011年中国崇左蔗糖业发展大会》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357426A (en) * | 2014-10-13 | 2015-02-18 | 华南理工大学 | Compound enzyme for cane molasses fermentation as well as preparation method and application thereof |
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Application publication date: 20111221 |