CN102172250A - Preparation method and application of eauveria-spinosad suspending agent - Google Patents

Abstract

The invention relates to a preparation method and application of a beauveria bassiana-spinosyn oil suspension. The invention is an oil suspension which takes Beauveria bassiana and spinosad as active ingredients and is mainly used for the prevention and control of agricultural and forestry pests such as potato beetles and locusts. (1) Containing active ingredients Beauveria bassiana and spinosad as biological pesticides, the rest are permitted and acceptable auxiliary components in pesticides; (2) The mass ratio of Beauveria bassiana and spinosad is 30 : 1 to 30: 2, the mass percent content of the active ingredient in the preparation is 3.1% to 10%. Advantages: This product can be miscible with oil or water, the persistent effect of Beauveria bassiana and the relative quick-acting effect of spinosad are complementary, and it can also treat a variety of crop pests; it has synergistic effect, good permeability, sticky Strong adhesion, anti-evaporation and other advantages.

Description

Preparation method and application of beauveria bassiana-spinosyn oil suspension

technical field

The invention relates to a microbial oil suspension, specifically an oil suspension with Beauveria bassiana and spinosad as active ingredients, which is mainly used for the prevention and control of potato beetles, locusts and other pests.

Background technique

Beauveria is the most common entomopathogenic fungus, which can infect more than 700 kinds of insects and mites, reproduce in large numbers in the body of the host, change the body fluid discovery function, and cause metabolic disorders and even death. The distribution range of Beauveria bassiana is very wide. Beauveria bassiana has been found in mountains ranging from a few meters above sea level to more than 2000 meters. Beauveria bassiana has strong pathogenicity and is safe for human and animal crops. One of the entomopathogenic fungi.

Beauveria bassiana has long been used by researchers as an important means of pest control because of its wide host range, strong prevalence, and safety to humans and animals. Slow speed, control effect is greatly affected by environmental conditions and other limiting factors.

At present, the conidia preparations of Beauveria bassiana that have been successfully developed in China include powder, wettable powder, granule and oil suspension. 14 kinds of preparation products have been registered in foreign countries, but in practical applications, most of the conidia preparations of Beauveria bassiana still use solid preparations such as powders, which have low content of active ingredients and large volume, which poses problems for transportation, storage and use. and due to the hydrophobicity of the Beauveria bassiana conidia, the amount of bacterial powder in use is large, the adhesion is not good, and the control effect is very unstable.

Spinosad is a low-toxic, high-efficiency, broad-spectrum, and low-residue insecticide. It has rapid contact and stomach toxicity to pests, and has a strong penetration effect on leaves, which can effectively control scale. Pests of the orders Diptera, Diptera and Thysanoptera. Suitable for use on vegetables, fruit trees, gardens, and crops.

Oil suspension concentrate is a highly dispersed and stable suspension system formed by one or more active ingredients of pesticides in a non-aqueous dispersion. Compared with water suspension concentrates, wettable powders, water dispersible granules and other dosage forms, it has the advantages of good permeability, strong adhesion, anti-evaporation, and storage resistance.

Contents of the invention

The object of the present invention is to provide a kind of Beauveria bassiana-spinosyn oil suspension. This oil suspension has integrated the respective advantages of the existing fungal oil suspension (such as the Beauveria bassiana products obtained from the Ministry of Agriculture's pesticide temporary registration number: LS20091079) and the water emulsion, and is a low-cost, easy-to-use Beauveria bassiana-spinosyn oil suspension.

The object of the present invention is achieved like this: the composition ratio of a kind of Beauveria bassiana-spinosyn oil suspension concentrate is that Beauveria bassiana high sporopollen accounts for 1%～50%, and spinosad accounts for 0.1% by mass percent. % to 1%, surfactants account for 0.5% to 8%, and suspended oils account for 40% to 70%.

The mass percent of each component is:

The above-mentioned high sporopollen of Beauveria bassiana is high sporopollen of Beauveria bassiana, the spore content is greater than 1× ¹⁰¹¹ /g, and the viable spore rate is greater than 85%; the above-mentioned surfactant is polyglycerol ricinoleate; the above-mentioned solvent The oil is polymerized linseed oil with an active content greater than 85%.

1. Preparation of high sporopollen of Beauveria bassiana

Get the Beauveria bassiana strain (ACCC32445, obtained from the China Agricultural Microorganism Culture Collection Management Center) and insert it into the commonly used sporulation medium for sterilization (YPDCA, YPDCA is the abbreviation of the main component, Y: yeast powder, P: peptone, D: glucose, C: corn extract powder A: agar) solid plate for activation treatment; scrape the activated strains to make a suspension and add liquid sporulation medium (YPDC) to expand the strain culture After 48 hours, connect it to the sterilized liquid medium in the fermenter for liquid fermentation; after 24 hours of cultivation, connect it to the sterilized solid culture material, mix well, and then enter the culture room for solid fermentation After the solid culture is spore-produced, it is sent to the drying room for drying treatment. After the solid culture is completely dried, the high sporopollen is extracted on a closed extraction machine, and finally the high sporopollen of Beauveria bassiana is obtained. The process flow is shown in Figure 1 .

2. Preparation of Beauveria bassiana-spinosyn oil suspension

According to the formula ratio, add Beauveria bassiana high sporopollen and emulsified oil into the mixing kettle, and carry out high-shear emulsification stirring (8000r/min) with a high-shear emulsifier for 10-15 minutes to fully mix the materials evenly; The product, add thickener and surfactant and continue high-shear emulsification stirring (8000r/min) in the mixing kettle, 10～15min, the product obtained adds the original drug of spinosad (produced by Dow Agricultural Science and Technology Company) , purity > 90%) carry out final high-shear emulsification stirring (8000r/min), 15～20min, obtain Beauveria bassiana-spinosad oil suspension concentrate, technological process is shown in accompanying drawing 2.

3. Application of Beauveria bassiana-spinosad oil suspension concentrate

The Beauveria bassiana-Multiple oil-killing suspension of the present invention is an oil suspension formulation. Because of the addition of a surfactant with good compatibility, adding water can form the state of oil-in-water and oil-wrapped spores during use, and sufficient water can promote the growth of spores. After germination, the oil-coated spores contact the host epidermis after the water evaporates, and have the characteristics of an oil suspension. Therefore, the Beauveria bassiana spore oil suspension concentrate has the characteristics that the oil suspension concentrate is less affected by the environment.

The above-mentioned Beauveria bassiana spore oil suspension is easy to use, it can be diluted with water, or oil can be used according to the needs, and a variety of application methods can be used; the specific method is: dilute 1 to 10 times with oil or water and spray with ultra-low volume technology ; Or use low-dose spray after diluting 10-100 times with water; or use constant spray after diluting 500-1000 times with water. When spraying with ultra-low volume technology, the dilution ratio with water is preferably 3 to 7 times; when spraying with low volume technology, the dilution ratio with water is preferably 10 to 30 times; when spraying with conventional technology, the dilution ratio with water is preferably 500 to 800 times.

The present invention has the following advantages:

(1) The product of the present invention has overcome the shortcoming that single use of Beauveria bassiana is slow in efficacy due to the adoption of these two single agents of spinosad and Beauveria bassiana, and has combined the original advantages of each single agent. It has obvious toxicity synergies to potato beetles, locusts and other pests, which can greatly improve the insecticidal effect and significantly shorten the onset time.

(2) In the compounded components of the product of the present invention, the dosage ratio of spinosad is less than that used alone, which not only reduces the toxicity and residual rate of the compound preparation, is beneficial to the protection of the ecological environment, but also reduces its production cost , has broad market prospects.

(3) The Beauveria bassiana-spinosad oil suspension of the present invention has a stable control effect and is less affected by the environment. Because the preparation contains surfactant, water emulsion is formed after adding water in the field, and the spores are wrapped in oil droplets, which has the characteristics that the oil suspension is less affected by the environment. At the same time, a large amount of water around can promote the germination of spores, thereby reducing the preparation time. Dependence on ambient humidity.

(4) The surfactant of the product of the present invention is non-ionic, has good compatibility with fungal spores in the oil suspension, has no obvious impact on the storage period of the oil suspension, and retains the characteristics of a long storage period of the oil suspension.

The beneficial effects of the present invention are

The invention relates to a preparation method and application of a beauveria bassiana-spinosyn oil suspension. The invention is an oil suspension which takes Beauveria bassiana and spinosad as active ingredients and is mainly used for the prevention and control of agricultural and forestry pests such as potato beetles and locusts. (1) Containing active ingredients Beauveria bassiana and spinosad as biological pesticides, the rest are permitted and acceptable auxiliary components in pesticides; (2) The mass ratio of Beauveria bassiana and spinosad is 30 : 1 to 30: 2, the mass percent content of the active ingredient in the preparation is 3.1% to 10%. The product involved in the present invention can be miscible with oil or water, and the persistent effect of Beauveria bassiana and the relative quick-acting property of spinosad have obtained complementary advantages, and one medicament can prevent and treat various crop pests; Good performance, strong adhesion, anti-evaporation and other advantages.

Description of drawings

Fig. 1 Process flow chart for the preparation of high sporopollen of Beauveria bassiana.

Fig. 2 is a flow chart of the preparation of Beauveria bassiana-spinosad oil suspension concentrate.

Detailed ways

In order to better understand the essence of the invention, the technical content of the invention is described in detail below with examples, but the prevention and treatment application of the present invention is not limited thereto. Percentages in the following examples are percentages in the formulation.

Take 3.2% Beauveria bassiana-spinosad oil suspension concentrate as an example, using examples of different concentrations

According to the formula requirements, add Beauveria bassiana high sporopollen and emulsified oil into the mixing kettle, and stir in a high-shear emulsifier (Shanghai Weiguang Machinery Manufacturing Co., Ltd. high-shear mixing emulsifier 100LX) (8000r/min) for 10~ 15min, to make the materials fully mixed evenly; add the thickener and surfactant to the mixing kettle for the mixed product, stir in a high-shear emulsifier (8000r/min) for 10-15min, and add more Carry out final high-shear emulsification and stirring (8000r/min) for 15 to 20 minutes to obtain the oil suspension concentrate, and after passing the inspection, carry out metering and packaging to obtain the finished product.

B-101 (Beijing Zhonghuan Yida Agricultural Science and Technology Development Co., Ltd.) can be used as surfactant to replace polyglycerol ricinoleate; thickener can be used to replace sodium octenyl starch with carboxymethyl cellulose sodium; solvent oil can be used as rapeseed oil or Soybean oil is substituted for polymerized linseed oil.

Use 500 g/mu of this mixture to spray evenly with water to prevent and control potato beetles, the control effect reaches 92% after 5 days of application, and reaches 94.2% on the 15th day.

Use 250 g/mu of this mixture to spray evenly with water to prevent and control potato beetles. The control effect reaches 87.3% after 5 days of spraying, and reaches 90% on the 15th day.

Water was used as blank control, and the control effect was 0.

The experimental results show that Beauveria bassiana can quickly kill a large number of potato beetle larvae after the application of the compound oil suspension, and can form a cadaveric rate of 20%. After one month of application, the infection rate of the vest in the soil was investigated. The rate of zombies in the soil was 19%. It has good quick-kill and continuous control effect.

Claims (5)

1. white muscardine fungi-multiple killing teichomycin oil-suspending agent preparation method is characterized in that:

(1) contain active ingredient biopesticide beauveria bassiana and multiple killing teichomycin, all the other are to allow in the agricultural chemicals to use and acceptable auxiliary element surfactant, thickener and solvent naphtha;

(2) mass ratio of beauveria bassiana and multiple killing teichomycin was at 30: 1～30: 2, and the mass percentage content of active ingredient in preparation is 2%～10%.

2. a kind of white muscardine fungi according to claim 1-multiple killing teichomycin oil-suspending agent is characterized in that the mass percent of each component is:

3. according to claim 1 and 2 described a kind of white muscardine fungi-multiple killing teichomycin oil-suspending agents, it is characterized in that its preferred ingredient and mass percent thereof are:

4. according to the described a kind of white muscardine fungi of claim 1-3-multiple killing teichomycin oil-suspending agent, it is characterized in that surfactant can substitute polyglycereol castor-oil plant alcohol ester with B-101; Thickener can substitute the octenyl Starch Sodium with sodium carboxymethylcellulose; Solvent naphtha can substitute the polymerization linseed oil with rapeseed oil or soybean oil.

5. the application of white muscardine fungi-multiple killing teichomycin oil-suspending agent is characterized in that this oil-suspending agent is mainly used in various pests such as control colorado potato beetle, locust.

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