CN102144767A - Method for extracting rebaudiodside A (RA) with content of 80% - Google Patents
Method for extracting rebaudiodside A (RA) with content of 80% Download PDFInfo
- Publication number
- CN102144767A CN102144767A CN2010101081351A CN201010108135A CN102144767A CN 102144767 A CN102144767 A CN 102144767A CN 2010101081351 A CN2010101081351 A CN 2010101081351A CN 201010108135 A CN201010108135 A CN 201010108135A CN 102144767 A CN102144767 A CN 102144767A
- Authority
- CN
- China
- Prior art keywords
- content
- solvent
- solid
- filter
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Saccharide Compounds (AREA)
- Vaporization, Distillation, Condensation, Sublimation, And Cold Traps (AREA)
- Seasonings (AREA)
Abstract
The invention discloses a method for extracting rebaudiodside A (RA) with a content of 80%, belonging to the technical field of food additive production. The RA is a component with the best taste in stevioside; and due to different processes and methods, the traditional production processes generally have the problems of low conversion rate, high energy consumption, high cost, unstable control of effective constituents and the like. The method provided by the invention comprises the steps of: mixing solvents such as methanol and the like according to a certain proportion; then mixing the mixed solvents with RA with low purity according to a certain proportion; carrying out solid-liquid separation after dissolving and agitating; and drying or washing and then drying obtained solid to obtain RA with high purity, wherein the content of the RA can be up to more than 80% and the conversion rate of the RA is up to more than 80%. The method for producing the RA with high purity has the advantages of simple process, low energy consumption, stable RA purity, no lingering bitterness and the like and is suitable for industrialized production.
Description
Technical field
The invention belongs to the food additives production technical field.
Background technology
Stevioside is that a kind of high sugariness that extraction obtains from STEVIA REBAUDIANA is hanged down heat energy, flavor matter is good and the natural low-heat sugar source of safety non-toxic, and its sugariness is 150～300 times of sucrose, can substitute asccharin or sucrose, is applied in various food, the beverage.Stevioside can also prevent diseases such as diabetes, obesity and pediatric saprodontia, is patient's desirable sweetener.But the bitter taste of stevioside influences its popularization and utilization always, and Japan and Korea S adopt the method for transferase and hydrolaseization to improve the bitter aftertaste of stevioside; Not enough is the side effect sugariness multiple decline often that brings, cost up.Cause the difficulty in the application equally.But progressively increase along with the natural sweetener market demand, the back bitter taste that more and more enterprises begins to pay close attention to research and solves stevia rebaudianum, people attempt the stevia rebaudianum product of refining refinement Gao Laibaodi glycosides content, get rid of bad composition steviobioside of mouthfeel and Du Ke glucoside, sugariness can up to 350-400 doubly have been removed the back bitter taste of stevia rebaudianum simultaneously like this.At present, some enterprises of Korea S, Malaysia, the U.S., China etc. can be purified to the Lai Baodi glycosides more than 80%, but because the difference of technology and method separately, the ubiquity conversion ratio is low, the energy resource consumption height, cost height, drawbacks such as active ingredient control instability.
Summary of the invention
In order to overcome the above problems, the invention provides the extracting method of a kind of production 80% content Lai Baodi glycosides A, this method process route is simple, and is less demanding to equipment, and have advantages such as low energy consumption, high production, stable, the thorough removal of RA purity back bitter taste, be fit to suitability for industrialized production.Concrete technical scheme is as follows, the extracting method of a kind of production 80% content Lai Baodi glycosides A, and it comprises following step:
(1) the solvent preparatory stage is methyl alcohol with methyl alcohol, water in the ratio of solvent: water (6～10: 0～4).
(2) mix stages: the low-purity stevioside was mixed than 1: 3～1: 15 by plastid with the methyl alcohol mixed solvent.
(3) agitation phases:, and keep 1～4h with the said mixture stirring and dissolving.
(4) the placement stage: will stir the back mixture and fully separate out at the placement of the temperature below 30 ℃ 0～24h RA.
(5) Separation of Solid and Liquid: carry out Separation of Solid and Liquid with method centrifugal or that filter, filter that aperture of filter material is less than 50 μ m or greater than 500 orders.
(6) liquids of separating is reclaimed 1～4h in boiling temperature, should place 0～96h below 30 ℃.
(7) mixed liquor is placed to separate out and is finished the back and carry out Separation of Solid and Liquid with method centrifugal or that filter, and the filtration aperture of filter material is less than 50 μ m or more than 500 orders.
(8) method that the solid content that separates is done with oven dry or spray is carried out drying.
The specific embodiment
Embodiment 1
(1) the solvent preparatory stage is methyl alcohol with methyl alcohol, water in the ratio of solvent: water (9: 1).
(2) mix stages: the low-purity stevioside was mixed than 1: 15 by plastid with the methyl alcohol mixed solvent.
(3) agitation phases:, and keep 1h with the said mixture stirring and dissolving.
(4) the placement stage: will stir the back mixture and place 6h in the temperature below 30 ℃.
(5) Separation of Solid and Liquid: carry out Separation of Solid and Liquid with the method for filtering, filter filter material 500 purpose filter clothes.
(6) liquids of separating is reclaimed 6h in boiling temperature, reclaim liquid and should fully separate out at the 72h of placement below 30 ℃ A.
(7) carry out Separation of Solid and Liquid with the method for filtering, filter filter material 500 purpose filter clothes.
(8) solid content that separates is dried with infrared drying oven.
Embodiment 2
(1) the solvent preparatory stage is methyl alcohol with methyl alcohol, water in the ratio of solvent: water (7: 3).
(2) mix stages: the low-purity stevioside was mixed than 1: 6 by plastid with the methyl alcohol mixed solvent.
(3) agitation phases:, and keep 2h with the said mixture stirring and dissolving.
(4) the placement stage: will stir the back mixture and place 12h in the temperature below 30 ℃.
(5) Separation of Solid and Liquid: carry out Separation of Solid and Liquid with the method for filtering, filter filter material 500 purpose filter clothes.
(6) liquids of separating is reclaimed 3h in boiling temperature, reclaim liquid and should fully separate out at the 48h of placement below 15 ℃ A.
(7) carry out Separation of Solid and Liquid with the method for filtering, filter filter material 500 purpose filter clothes.
(8) with behind the solid content deionized water dissolving that separates, volatilize organic solvent, the dried machine drying of spray dryer.
Claims (1)
1. the extracting method of a content Lai Baodi glycosides A is characterized in that it comprises the following aspects:
(1) raw material: the raw material that this technology is used can be that RA content is at the steviol glycoside more than 20%.
(2) solvent: the solvent that this technology is used is a methyl alcohol, water etc.
(3) ratio with the described solvent of claim (2) is a methyl alcohol: water (6～10: 0～4).
(4) be 3～10: 1 with described mixed solvent of claim (3) and the described material rate of claim (1), after raw material and the solvent, at stirring at normal temperature 1～4h.
(5) finish with the stirring under the claim (3), (4) after, mixed liquor should placed 0～24h below 30 ℃.
(6) with the mixed liquor under the claim (5), to place to separate out and finish the back and carry out Separation of Solid and Liquid with method centrifugal or that filter, the filtration aperture of filter material is less than 50 μ m or more than 500 orders.
(7) liquids of separating is reclaimed 1～8h in boiling temperature, reclaim liquid and should place 0～96h below 30 ℃.
(8) with the mixed liquor under the claim (7), to place to separate out and finish the back and carry out Separation of Solid and Liquid with method centrifugal or that filter, the filtration aperture of filter material is less than 50 μ m or more than 500 orders.
(9) method that the solid content that separates is done with oven dry or spray is carried out drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010108135A CN102144767B (en) | 2010-02-10 | 2010-02-10 | Method for extracting rebaudiodside A (RA) with content of 80% |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010108135A CN102144767B (en) | 2010-02-10 | 2010-02-10 | Method for extracting rebaudiodside A (RA) with content of 80% |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102144767A true CN102144767A (en) | 2011-08-10 |
| CN102144767B CN102144767B (en) | 2012-10-10 |
Family
ID=44419403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010108135A Active CN102144767B (en) | 2010-02-10 | 2010-02-10 | Method for extracting rebaudiodside A (RA) with content of 80% |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102144767B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56121453A (en) * | 1980-02-27 | 1981-09-24 | Ajinomoto Co Inc | Separation of stevioside and rebaudioside a |
| US5962678A (en) * | 1996-09-13 | 1999-10-05 | Alberta Research Council | Method of extracting selected sweet glycosides from the Stevia rebaudiana plant |
| CN1243835A (en) * | 1998-08-05 | 2000-02-09 | 黑龙江垦区海林甜菊糖有限责任公司 | Process for extracting rebandioside A |
| US20060083838A1 (en) * | 2004-10-18 | 2006-04-20 | Sweet Aloha Farms, L.L.C. | High yield method of producing pure rebaudioside A |
| CN101200480A (en) * | 2006-12-15 | 2008-06-18 | 成都华高药业有限公司 | Rebaudioside A and extraction method thereof |
| CN101591366A (en) * | 2009-07-07 | 2009-12-02 | 天津美伦医药集团有限公司 | The technology of separating high-purity rebaudioside A |
-
2010
- 2010-02-10 CN CN201010108135A patent/CN102144767B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56121453A (en) * | 1980-02-27 | 1981-09-24 | Ajinomoto Co Inc | Separation of stevioside and rebaudioside a |
| US5962678A (en) * | 1996-09-13 | 1999-10-05 | Alberta Research Council | Method of extracting selected sweet glycosides from the Stevia rebaudiana plant |
| CN1243835A (en) * | 1998-08-05 | 2000-02-09 | 黑龙江垦区海林甜菊糖有限责任公司 | Process for extracting rebandioside A |
| US20060083838A1 (en) * | 2004-10-18 | 2006-04-20 | Sweet Aloha Farms, L.L.C. | High yield method of producing pure rebaudioside A |
| CN101200480A (en) * | 2006-12-15 | 2008-06-18 | 成都华高药业有限公司 | Rebaudioside A and extraction method thereof |
| CN101591366A (en) * | 2009-07-07 | 2009-12-02 | 天津美伦医药集团有限公司 | The technology of separating high-purity rebaudioside A |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102144767B (en) | 2012-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101690573B (en) | Production method of fructus monordicae extract with over 60% of mogroside V content | |
| CN101633676B (en) | Method for preparing high-purity stachyose by using plant chromatographic separation technology | |
| CN106146574B (en) | A kind of method that microwave-ULTRASONIC COMPLEX extracts steviol glycoside in stevioside leaf | |
| CN108752231B (en) | Method for extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenol | |
| CN102924544B (en) | Method for preparing stevioside and chlorogenic acid from stevia step by step | |
| CN102321128B (en) | Combined extraction and purification technique for multiple active ingredients of polygonum multiflorum | |
| CN102702284A (en) | Production process of high-purity sweet tea glycosides | |
| CN108516997A (en) | A method of extracting Rubusoside from sweet tea | |
| CN106810622B (en) | A method of it extracting neohesperidin from the dried immature fruit of citron orange and the dried immature fruit of citron orange is comprehensively utilized | |
| CN106046075A (en) | Method for extracting stevioside from stevia rebaudiana leaves | |
| CN102161689A (en) | Method for extracting tea saponin from oil-tea-cake | |
| CN104388498A (en) | Method for producing total glycoside 80% enzymes for improving stevioside taste quality | |
| CN109674843A (en) | A kind of method for extraction and purification of dried fructus momordicae comprehensive utilization | |
| CN101891781A (en) | Method for preparing high-purity gardenoside | |
| CN101407535B (en) | Preparation of high-purity Momordica grosvenori mogroside V | |
| CN109180755A (en) | A method of extracting Rubusoside from sweet tea | |
| CN105399795B (en) | Method for extracting astragaloside from radix astragali | |
| CN102286058A (en) | Method for preparing glycyrrhizic acid with high purity | |
| CN102146106A (en) | Method for extracting 95 percent of rebaudioside A | |
| CN104725444A (en) | Method for efficiently preparing high-purity rebaudioside A | |
| CN102827306A (en) | Method for preparing pectin from capsicum residue | |
| CN101759731B (en) | Extraction method of linseed gum and secoisolariciresin-ol diglucoside | |
| CN105153252A (en) | Method for extracting rubusoside from strigose hydrangea leaves | |
| CN110229201B (en) | Process method for preparing high-purity stevioside RM | |
| CN103059078A (en) | Method for extracting rebaudioside-A with 99 percent of content from stevioside |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20110810 Assignee: QUFU SHENG REN PHARMACEUTICAL CO., LTD. Assignor: Saint-Ouen Shandong Pharmaceutical Co., Ltd. Contract record no.: 2014370000081 Denomination of invention: A method for extracting 80% content of rebaudioside A Granted publication date: 20121010 License type: Exclusive License Record date: 20140508 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model |