CN102115717B - 低核酸酵母产品、其制备方法以及包含该低核酸酵母的减肥产品 - Google Patents
低核酸酵母产品、其制备方法以及包含该低核酸酵母的减肥产品 Download PDFInfo
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Abstract
本发明涉及一种低核酸酵母产品及其制备方法。一种低核酸酵母产品,包含蛋白质45~55%,核酸0.05~1.0%,钙100~5000ppm,铁30~1000ppm,锌30~1000ppm。本发明还涉及该低核酸酵母产品的制备方法:取酵母用水配制成质量百分比浓度为5-10%的溶液;调节酵母溶液至pH=6.0-10.0;将酵母溶液在60-100℃保温1-6小时;再调节酵母溶液至pH=5.0~7.0;分离,洗涤,干燥酵母溶液,获得低核酸酵母产品。本发明的方法简便有效地降低了酵母中核酸含量,即降低食用该酵母及以该酵母为原料生产的相关产品引发痛风的危险,并且保留了原有酵母中的营养价值。本发明还涉及一种包括该低核酸酵母的减肥产品,低热量、高营养同时还具有促进脂肪分解的作用,安全有效。
Description
技术领域
本发明涉及一种低核酸酵母产品及其制备方法,以及一种包括该低核酸酵母的减肥产品。
背景技术
酵母作为一种食品开始走进人们的日常生活,越来越多的酵母产品得以问世。现在市场上大部分酵母食品是以生产啤酒后的啤酒酵母泥作为原料,开发成不同的食品。现有技术主要是通过对啤酒酵母进行脱苦处理然后直接干燥就给人食用,或者在干燥前强化一些矿物质、微量元素,做成营养素补充剂。
近年来很多人反映食用该类产品后容易引发痛风,其中引发痛风的主要成份是核酸。酵母中核酸含量一般在5%左右,食用以这种酵母为主要原料的产品后,容易引发痛风。核酸类保健食品申报与审评规定“核酸类保健食品说明书及标签中的‘不适宜人群’除按保健食品相关规定标注外,应明确标注出‘痛风患者’”。
将啤酒酵母泥直接干燥后给人食用,或以酵母为载体做成各种营养素补充剂。
现有技术中对酵母的核酸及核酸含量没有控制,导致一些人食用此类产品后引发痛风,给消费者带来很大的痛苦。
浓盐法、稀碱法可以将酵母中的核酸提取出来,但是这些方法致使酵母的营养损失严重,生产的酵母没有食用或保健的价值。
发明内容
本发明主要解决的技术问题是降低酵母中的核酸,保留酵母中的蛋白质、钙、铁、锌以及酵母的营养价值。
本发明提供的低核酸酵母产品的制备方法是以面用酵母、酿酒酵母、啤酒酵母(或经脱苦的啤酒酵母泥)为原料,利用酵母在一定浓度、温度和pH下酵母细胞壁有较好的通透性原理将酵母中的核酸溶解在水中,通过分离洗涤除去酵母中的核酸但不损失酵母中的蛋白质。经该工艺处理后酵母中核酸由原来5%左右下降为0.1~1.0%,远远低于现有酵母中核酸,同时保留该酵母中的蛋白质、钙、铁、锌等营养元素。以该酵母生产出来的食品没有较浓的酵母味,产品蛋白质高、对人体安全(不会引发痛风)。具体工艺流程图如图1所示。
本发明所提供的低核酸酵母的主要成分重量百分含量为:蛋白质45~55%,核酸(以RNA计)0.05~1.0%,钙100~5000ppm,铁30~1000ppm,锌30~1000ppm。该酵母是一种干酵母,气味较淡。
本发明所提供的一种低核酸酵母的制备方法,具体步骤包括:
1)取酵母用水配制成质量百分比浓度为5-10%的溶液,优选浓度7~10%,最优8%;
2)调节步骤1)所得酵母溶液至pH=6.0-10.0,优选pH=7.0-9.0,最优选pH=8.5。
3)将步骤2)所得酵母溶液在60-100℃保温1-6小时,优选温度80-100℃,保温时间2-4小时,最优选95℃保温3小时;
4)再调节步骤3)所得酵母溶液至pH=6.0;
5)分离,洗涤,干燥步骤4)所得酵母溶液,获得低核酸酵母。
其中步骤2)、步骤4)中调节pH的酸可选用硫酸、盐酸、硝酸、磷酸等,碱可用氢氧化钠、氢氧化钾等。
具体地,所述步骤5)中的分离步骤可根据具体情况任选现有技术的分离设备进行,如叠片式分离机。
本发明的方法简便可行,成本低,同时利用本发明的方法有效地降低了酵母中核酸含量,即降低食用该酵母及以该酵母为原料生产的相关产品引发痛风的危险,并且保留了原有酵母中的营养价值。本发明制备的低核酸酵母产品仍是完整的酵母细胞,以有效保留了细胞中的营养物质。
本发明解决的另一个技术问题是提供了一种安全、有效的减肥产品,
本发明所涉及的减肥产品,是以低核酸酵母为原料辅以丙酮酸钙、L-肉碱,同时强化维生素B1、B2、B6、B12、叶酸、烟酸、泛酸、维生素A、维生素C、维生素D等混合而成。
该减肥产品的主要成分重量百分含量为:低核酸酵母含量为70~99%,丙酮酸钙含量0.1~10%,L-肉碱含量0.1~15%,营养元素的含量为:维生素B110~1000ppm,维生素B210~1000ppm,维生素B610~1000ppm,维生素B120.1~50ppm,叶酸10~1000ppm,烟酸10~5000ppm,泛酸50~5000ppm,维生素A10~500ppm,维生素C1000~5000ppm,维生素D0.1~10ppm。
本发明的减肥食品是一种低热量、高营养同时还具有促进脂肪分解的作用,是一款安全、有效的减肥食品。
附图说明
图1为本发明制备低核酸酵母产品的主要工艺流程图。
具体实施方式
以下通过实施例来详细描述本发明。
一、制备低核酸酵母产品
实施例1
取安琪酵母股份有限公司生产的安琪酿酒酵母,加入去离子水,配制成质量百分比浓度为5%的溶液;用硫酸和氢氧化钠调节该酵母溶液至pH=6.0;接着,将该酵母溶液在60℃保温1小时;再调节该酵母溶液至pH=5.0;用叠片式分离机分离,收集分离的重相,然后在所得重相内加水,加水量与所得重相体积相同,搅拌30分钟后再用分离机进行分离,将所得重相进行喷雾干燥,获得低核酸酵母样品1#。
实施例2
取安琪酵母股份有限公司生产的安琪啤酒酵母,加入去离子水,配制成质量百分比浓度为7%的溶液;用硫酸和氢氧化钠调节该酵母溶液至pH=9;接着,将该酵母溶液在80℃保温4小时;再调节该酵母溶液至pH=6.0;用叠片式分离机分离,收集分离的重相,然后在所得重相内加水,加水量与所得重相体积相同,搅拌30分钟后再用分离机进行分离,将所得重相进行滚筒干燥,获得低核酸酵母样品2#。
实施例3
取安琪酵母股份有限公司生产的安琪面用酵母,加入去离子水,配制成质量百分比浓度为5%的溶液;用硫酸和氢氧化钠调节该酵母溶液至pH=6.0;接着,将该酵母溶液在60℃保温1小时;再调节该酵母溶液至pH=7.0;用叠片式分离机分离,收集分离的重相,然后在所得重相内加水,加水量与所得重相体积相同,搅拌30分钟后再用分离机进行分离,将所得重相进行喷雾干燥,获得低核酸酵母样品1#。
实施例4
取安琪酵母股份有限公司生产的安琪面用酵母,加入去离子水,配制成质量百分比浓度为8%的溶液;用硫酸和氢氧化钠调节该酵母溶液至pH=8.5;接着,将该酵母溶液在95℃保温3小时;再调节该酵母溶液至pH=6.5;用叠片式分离机分离,收集分离的重相,然后在所得重相内加水,加水量与所得重相体积相同,搅拌30分钟后再用分离机进行分离,将所得重相进行喷雾干燥,获得低核酸酵母样品4#。
实施例5
取安琪酵母股份有限公司生产的安琪面用酵母,加入去离子水,配制成质量百分比浓度为10%的溶液;用硫酸和氢氧化钠调节该酵母溶液至pH=10;接着,将该酵母溶液在100℃保温6小时;再调节该酵母溶液至pH=6.2;用叠片式分离机分离,收集分离的重相,然后在所得重相内加水,加水量与所得重相体积相同,搅拌30分钟后再用分离机进行分离,将所得重相进行喷雾干燥,获得低核酸酵母样品5#。
实施例6
取安琪酵母股份有限公司生产的经脱苦后的啤酒酵母泥,加入去离子水,配制成质量百分比浓度为9%的溶液;用硫酸和氢氧化钠调节该酵母溶液至pH=7;接着,将该酵母溶液在85℃保温5小时;再调节该酵母溶液至pH=5.8;用叠片式分离机分离,收集分离的重相,然后在所得重相内加水,加水量与所得重相体积相同,搅拌30分钟后再用分离机进行分离,将所得重相进行喷雾干燥,获得低核酸酵母样品6#。
各实施例所得样品中各成分质量百分含量如下表所示:
核酸(以RNA计) | 蛋白质 | 钙(ppm) | 铁(ppm) | 锌(ppm) | |
样品1# | 1.0% | 45% | 1500 | 100 | 200 |
样品2# | 0.41% | 53% | 1607 | 300 | 390 |
样品3# | 0.22% | 51% | 1721 | 500 | 400 |
样品4# | 0.05% | 54% | 3827 | 700 | 930 |
样品5# | 0.6% | 55% | 5000 | 1000 | 1000 |
样品6# | 0.8% | 49% | 100 | 30 | 30 |
二、动物实验
将制备实施例中的低核酸酵母产品样品1#、2#、3#、4#、5#、6#、安琪面用酵母、安琪酿酒酵母、安琪啤酒酵母、啤酒酵母泥同时进行动物实验,经过30天的喂养实验发现,低核酸酵母组对实验大白鼠血清中尿酸影响不显著,而普通酵母对大白鼠血清尿酸水平影响显著,能显著升高大白鼠血清中尿酸。
本发明的低核酸酵母与普通酵母进行动物实验时,大白鼠体内血清中尿酸含量的变化如下表:
初始血清中尿酸 | 30天后血清中 |
含量(μmol/L) | 尿酸含量 | |
空白组 | 90.28±2.6 | 91.98±2.6 |
样品1# | 90.21±2.0 | 92.21±2.4 |
样品2# | 90.32±2.2 | 91.87±2.1 |
样品3# | 90.40±2.4 | 92.43±2.7 |
样品4# | 90.36±2.3 | 91.34±2.3 |
样品5# | 90.38±2.5 | 92.18±2.8 |
样品6# | 90.41±2.7 | 92.24±2.4 |
面用酵母 | 90.29±2.2 | 105.70±3.8 |
酿酒酵母 | 90.52±2.1 | 106.27±4.5 |
啤酒酵母 | 90.64±2.8 | 118.67±4.9 |
啤酒酵母泥 | 90.68±2.9 | 121.42±5.0 |
因此,根据本发明方法生产的低核酸酵母产品组相比安琪面用酵母、安琪酿酒酵母、安琪啤酒酵母、啤酒酵母泥组对高尿酸患者或痛风患者的影响要小的多,因此更安全。
本发明经过试制及生产,现已有符合企业标准的合格产品。
三、制备减肥产品
实施例1
产品配方(千克/吨)
低核酸酵母 90.049
丙酮酸钙 4.0
L-肉碱 4.0
维生素B1 0.1
维生素B2 0.1
维生素B6 0.1
维生素B12 0.0005
叶酸 0.1
烟酸 0.5
泛酸 0.5
维生素A 0.05
维生素C 0.5
维生素D 0.0005
生产工艺:
将以上配方中的原料按配方称量,接着一起投入三维混合机中混合40分钟,然后用粉末包装机包装成300g/瓶,然后进行大包入库。
Claims (8)
1.一种低核酸酵母,其特征在于,蛋白质含量为45~55%,核酸含量为0.05~1.0%,钙含量为100~5000ppm,铁含量为30~1000ppm,锌含量为30~1000ppm;所述酵母为酿酒酵母。
2.一种如权利要求1所述的低核酸酵母的制备方法,包括:
1)酵母用水配制成质量百分比浓度为5-10%的溶液;
2)调节步骤1)所得酵母溶液至pH=6.0-10.0;
3)将步骤2)所得酵母溶液在60-100℃保温1-6小时;
4)再调节步骤3)所得酵母溶液至pH=5.0~7.0;
5)分离,洗涤,干燥步骤4)所得酵母溶液,获得低核酸酵母产品。
3.一种如权利要求2所述的低核酸酵母的制备方法,其特征在于,所述步骤1)中用水配制成质量百分比浓度为7~10%。
4.一种如权利要求2所述的低核酸酵母的制备方法,其特征在于,所述步骤1)中用水配制成质量百分比浓度为8%。
5.一种如权利要求2所述的低核酸酵母的制备方法,其特征在于,所述步骤2)中调节步骤1)所得酵母溶液至pH=7.0-9.0,
6.一种如权利要求5所述的低核酸酵母的制备方法,其特征在于,所述步骤2)中调节步骤1)所得酵母溶液至pH=8.5。
7.一种如权利要求2所述的低核酸酵母的制备方法,其特征在于,所述步骤3)将步骤2)所得酵母溶液在80-100℃保温2-4小时。
8.一种如权利要求7所述的低核酸酵母的制备方法,其特征在于,所述步骤3)将步骤2)所得酵母溶液在95℃保温3小时。
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