CN102093967B - Mink source enterococcus faecium and application thereof - Google Patents
Mink source enterococcus faecium and application thereof Download PDFInfo
- Publication number
- CN102093967B CN102093967B CN2010105696124A CN201010569612A CN102093967B CN 102093967 B CN102093967 B CN 102093967B CN 2010105696124 A CN2010105696124 A CN 2010105696124A CN 201010569612 A CN201010569612 A CN 201010569612A CN 102093967 B CN102093967 B CN 102093967B
- Authority
- CN
- China
- Prior art keywords
- mdf1104
- faecium
- mink
- enterococcus faecium
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000772415 Neovison vison Species 0.000 title claims abstract description 21
- 241000194031 Enterococcus faecium Species 0.000 title claims abstract description 8
- 230000037406 food intake Effects 0.000 claims description 7
- 235000019786 weight gain Nutrition 0.000 claims description 4
- 230000004584 weight gain Effects 0.000 claims description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 21
- 235000012000 cholesterol Nutrition 0.000 abstract description 10
- 206010012735 Diarrhoea Diseases 0.000 abstract description 7
- 150000001720 carbohydrates Chemical class 0.000 abstract description 5
- 230000036039 immunity Effects 0.000 abstract description 4
- 230000000593 degrading effect Effects 0.000 abstract description 3
- 230000001932 seasonal effect Effects 0.000 abstract description 3
- 235000021050 feed intake Nutrition 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 16
- 241000894006 Bacteria Species 0.000 description 13
- 230000002354 daily effect Effects 0.000 description 13
- 241000699666 Mus <mouse, genus> Species 0.000 description 10
- 239000006041 probiotic Substances 0.000 description 10
- 235000018291 probiotics Nutrition 0.000 description 10
- 230000015556 catabolic process Effects 0.000 description 9
- 238000006731 degradation reaction Methods 0.000 description 9
- 230000012010 growth Effects 0.000 description 9
- 235000014655 lactic acid Nutrition 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 210000000056 organ Anatomy 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 230000003203 everyday effect Effects 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 230000000529 probiotic effect Effects 0.000 description 5
- 239000000654 additive Substances 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 208000021017 Weight Gain Diseases 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000036737 immune function Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 238000003794 Gram staining Methods 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 244000005709 gut microbiome Species 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000021238 nutrient digestion Nutrition 0.000 description 2
- 230000010412 perfusion Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- XUCIJNAGGSZNQT-JHSLDZJXSA-N (R)-amygdalin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O[C@@H](C#N)C=2C=CC=CC=2)O1 XUCIJNAGGSZNQT-JHSLDZJXSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 208000021959 Abnormal metabolism Diseases 0.000 description 1
- ATWHGWYKSFRYBN-UHFFFAOYSA-N Amygdaloside Natural products O1C(=O)C2(C)CCCC(C3CC4)(C)C2C1OCC3(C1)CC4C1(O)COC1OC(CO)C(O)C(O)C1O ATWHGWYKSFRYBN-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 208000035150 Hypercholesterolemia Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- SRBFZHDQGSBBOR-QMKXCQHVSA-N alpha-L-arabinopyranose Chemical compound O[C@H]1CO[C@@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-QMKXCQHVSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001669 bursa of fabricius Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 239000012050 conventional carrier Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000004837 gut-associated lymphoid tissue Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000003405 ileum Anatomy 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 230000015788 innate immune response Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- QWIZNVHXZXRPDR-WSCXOGSTSA-N melezitose Chemical compound O([C@@]1(O[C@@H]([C@H]([C@@H]1O[C@@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O)CO)CO)[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O QWIZNVHXZXRPDR-WSCXOGSTSA-N 0.000 description 1
- 230000006371 metabolic abnormality Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 229940054441 o-phthalaldehyde Drugs 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- CQRYARSYNCAZFO-UHFFFAOYSA-N salicyl alcohol Chemical compound OCC1=CC=CC=C1O CQRYARSYNCAZFO-UHFFFAOYSA-N 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000010119 systemic immune function Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229960003487 xylose Drugs 0.000 description 1
Images
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to an enterococcus faecium and application thereof, in particular to an enterococcus faecium MDF1104 with the patent collection number of CGMCC (China General Microbiological Culture Collection) No.4237. The invention also relates to the application of the mink source enterococcus faecium MDF1104 on fermenting carbohydrate and degrading cholesterol, in particular to the single application or combination with the common carrier of the enterococcus faecium MDF1104 to increase mink feed intake and daily gain for the mink, enhance organism immunity performance, and prevent and cure mink seasonal diarrhea.
Description
Technical field
The present invention relates to a strain and in the mink body, separate the faecium that obtains, and be particularly related to its application in the furbearer daily ration.
Technical background
Probiotics is the probiotics preparation of bringing into play the work of beneficial effect by improving host's intestinal microflora balance, with its green safety, have no side effect, the advantage of noresidue progressively becomes the main force of substitute antibiotics class additive.The enterococcus spp bacterium distributes very wide at occurring in nature, common in some vegetables and plant, also is a kind of microorganism of normal presence in the humans and animals digestive tube, and main field planting is at ileum and colon position.Faecalis mainly concentrates on enterococcus faecalis and faecium as the application of probiotic bacterium, and faecium belongs to milk-acid bacteria, because its fast growth has the ability of sticking preferably, can produce lactic acid and some antimicrobial substances, is used as widely probiotics preparation.Studies show that the probiotic bacterium that faecium forms can improve the body weight gains of livestock and poultry childhood, improve the nutrient digestion rate, improve the price of deed, reduce the diarrhoea ratio, alleviation stress situation, reduces mortality ratio.Faecalis also can be participated in the metabolism of cholesterol directly except possessing above function, and abnormal metabolism is adjusted, and makes it to recover normal.Faecalis is widely used in broiler chicken, growing and fattening pigs, finished cattle, piglet and the milk cow production as probiotics.In addition, probiotics has obvious immunologic enhancement to body, can promote the growth of newborn infant's intestinal tract immune system, also can promote intestinal mucosa part and systemic immune function, specificity and nonspecific immune response all there are regulating effect, keep the normal level of body immunologic function when state of health.Although probiotic bacterium has above numerous immunoregulation effect, concerning bacterial classification, not only immune effect is variant between the kind, and also has different between bacterial strain and the bacterial strain.In addition, the aspects such as absorption time, supply mode and addition are also influential to the immunomodulatory effect.The host is also because of the difference of kind, age, physiological situation, living environment, and produces different effects.
Faecium extensively exists in environment, and belongs to the enteron aisle fungal component, is in the ascendance in 2 new born～3d of a lot of animals, and is a kind of amphimicrobian milk-acid bacteria, is very suitable for producing and using.Comprise manure enterococcin strain in existing a lot of products in the market, such as " Medilac-Vita " of Korea S, probiotic composition Ventrux, the Bioflorin that Germany and Sweden produce and Fiormil etc., its viable count is approximately 10
8-9Individual/the g product, the goods that contain at present faecium also have been widely used in during animal produces, the fodder additives of producing such as Korea S " ratio of greater inequality he " etc.The Diseases of Cow direct microorganism fodder that contains faecium of feeding can promote that forage digests in cud, increase milk yield.The beef cattle direct microorganism fodder that contains faecium of feeding can promote beef cattle to adapt to high fine fodder daily ration, prevents oxypathy.Using proper faecium not only can promote nutrient digestion to absorb, reduce serum cholesterol content, keep the intestinal microflora balance, can also regulate body's immunity, stimulate the growth of the immune organs such as thymus gland, spleen and the fabricius bursa, strengthen the activate the phagocytic capacity of scavenger cell, activation gut-associated lymphoid tissue, the generation of Promote immunity globulin A, the immunologic function of regulating body.
About lactic bacteria additive, research and application in furbearer produces are studied less both at home and abroad, the present invention is based on the prebiotic effect effect of probiotic additive, proposed a strain and derived from the interior safety of furbearer body, stable, highly active milk-acid bacteria, and its advantage function detected.
Summary of the invention
Separating lactic acid bacterium in the healthy adult mink enteron aisle, get small intestine contents 20g, add in the 100ml sterilized water, shaking table concussion 30min, particulate matter is fully dissolved, gradient dilution, utilize pour plate method to carry out separation and Culture, cultivate the bacterium colony that 72h selects to have molten calcium circle for 37 ℃, adopt the mode purifying of plate streaking, purifying 3-4 generation, microscopy in the access of the milk-acid bacteria behind purifying MRS liquid nutrient medium, detects its Microbiological Characteristics, such as morphology, the ability of physiology characteristic (table 1) and sugar decomposition metabolism (table 2) detects its vitro culture to the degradation function (table 3) of cholesterol.Carry out that mouse security (table 4) detects and on the impact of mink production performance (table 5).
This faecium MDF1104 on October 21st, 2010 in China Committee for Culture Collection of Microorganisms common micro-organisms center (address: No. 3, No. 1 institute in North Star West Road, Chaoyang District, BeiJing, China city, Institute of Microorganism, Academia Sinica) preservation, preserving number CGMCC No.4237.Its Classification And Nomenclature is: faecium, latin name: Enterococcusfaecium.
Description of drawings
To the degraded of cholesterol, X-coordinate is incubation time (unit: hour) to Fig. 1 faecium MDF1104 under condition of in vitro culture, and ordinate zou is degradation rate (%).
Further specify the present invention below in conjunction with embodiment and Tu:
The embodiment I
Faecium MDF1104 morphological observation, microscope inspection and physiological and biochemical index detect
Cultivate the faecium MDF1104 of 18-24h under the aseptic condition with the transfering loop picking, be applied on the clean slide glass, film-making, gramstaining carries out morphology and coloration result is observed.Under the aseptic condition faecium is inoculated under corresponding substratum or the culture condition, carries out the detection of physiological and biochemical index and observe, detected result sees Table 1
Table 1 faecium MDF1104 morphology and physics and chemistry test-results
| Detect index | Characteristic |
| Morphology | Sphere, two chainings or be dispersed in |
| Gramstaining | Positive |
| Form gemma | No |
| Catalase | - |
| Oxydase | - |
| Grow in the air | + |
| 65 ℃ of growths | + |
| 45 ℃ of growths | + |
| 15 ℃ of growths | + |
| PH value (cultivating 24h) | 4.29 |
| The growth of 6.5% sodium-chlor | + |
| The pH9.6 growth | + |
| The pH4.5 growth | + |
The embodiment II
Faecium MDF1104 detects different carbohydrate fermentation activities
Under the aseptic condition, a little is eugonic with the transfering loop picking, cultivate the faecium MDF1104 of 18-24h, inoculation enters in the different sugar fermentation tube of Beijing Luqiao Technology Co., Ltd.'s production, 37 ℃ of constant temperature culture 18-24h, observe the fermented liquid colour-change, to judge the fermentation situation to different carbohydrate.
The different carbohydrate fermentation activities of table 2 faecium MDF1104
| Detect index | Characteristic | Detect index | Characteristic |
| Glucose | + | Raffinose | + |
| Seminose | + | Sorbyl alcohol | + |
| Melizitose | - | Melibiose | + |
| Fructose | + | Semi-lactosi | + |
| Saligenin | + | N.F,USP MANNITOL | + |
| Amygdaloside | + | Pectinose | + |
| Ribose | + | Sunmorl N 60S | - |
| Trehalose | + | Sucrose | + |
| Wood sugar | + | Cellobiose | + |
| Rhamnosyl | + | Polychrom | + |
| Maltose | + | The glucose fermentation aerogenesis | - |
| Lactose | + |
By table 1,2 as can be known, faecium MDF1104, and Gram-positive can be grown in than wide temperature range, and it is strong that 24h cultivates acid producing ability, can extensively utilize the carbohydrate generate energy, is the good milk-acid bacteria of a strain proterties.
The embodiment III
Faecium MDF1104 in Process of in vitro to the Degradation of cholesterol
With the faecium MDF1104 after the purifying cultivation, inoculation enters in the MRS liquid nutrient medium of new preparation and sterilization, cultivates 18-24 hour for 37 ℃, every sampling in four hours, the centrifuging and taking supernatant utilizes o-phthalaldehyde method to measure the interior milk-acid bacteria of 24h to the degradation capability of cholesterol.
Table 3 faecium MDF1104 is to the degradation rate of cholesterol
| Sample time | Degrading rate of cholesterol % |
| 4 | 38.45 |
| 8 | 32.05 |
| 12 | 32.05 |
| 16 | 32.55 |
| 24 | 31.07 |
By table 3 and accompanying drawing 1 as can be known, faecium MDF1104, the cholesterol of under condition of in vitro culture, can degrading, but and in the equal efficient degradation of whole incubation period, average degradation rate reaches 33.23%, reaches in the logarithm division stage to be up to 38.45%, illustrates that thus mink source faecium MDF1104 has preferably degradation property to cholesterol, and vitro culture in the phase its degradation efficiency descend not quite, illustrate also that thus MDF1104 has its unique advantage to the degraded of hypercholesterolemia food or feed.
The embodiment IV
Faecium MDF1104 is on the impact of small white mouse security and production performance index
In faecium MDF1104 access 10ml liquid nutrient medium, 1%, 37 ℃ of cultivation of inoculum size 18h, centrifugal 10 minutes of 5000rmp/min is 10 with normal saline dilution
7Cfu/ml and 10
9Cfu/ml.Choose healthy adult Kunming white mouse, 15 every group, the MDF1104 diluent 0.3ml of experimental group every per os perfusion every day different concns, control group pours into the physiological saline of equivalent every day, and continous pouring 20 days was observed 10 days, observe every day, records the situation of searching for food, the body weight situation.Routine weighing, monitor the situation of searching for food every day, butchers experiment during 30d, weighing thymus gland, spleen weight calculates Immune Organs Index.
Table 4. faecium MDF1104 is to small white mouse security and production performance Index Influence
| Group | Dead number of elements | Average daily gain g | Average daily ingestion amount g | Immune Organs Index |
| Control group | 2 | 9.90 | 12.98 | 0.57％ |
| MDF1104-10 7 | 0 | 10.80 | 18.19 | 0.56％ |
| MDF1104-10 9 | 0 | 11.63 | 18.27 | 0.59％ |
As shown in Table 4, the faecium MDF1104 of mink source, with various dose perfusion mouse, dead mouse is not arranged, and daily ingestion amount and the average daily gain of adding MDF1104 group mouse all are significantly higher than control group, the Immune Organs Index low dose group is lower than control group, and high dose group is a little more than control group.Hence one can see that, faecium MDF1104, to small white mouse without lethality.And day weight gain and average daily ingestion amount to small white mouse have promoter action, and high dose group has the effect that promotes immune function of mice.
The embodiment V
Faecium MDF1104 is on the impact of mink production performance
By 1% inoculum size, in the access liquid nutrient medium, 37 ℃ of fermentation 24h choose healthy finishing period mink with faecium, and 20 every group, every of test group every day is raised 10 with feed
9The faecium MDF1104 of cfu/g dry-matter, control group does not add, routine weighing, monitor the situation of searching for food every day, follows the tracks of every group of mink diarrhoea situation, fed continuously 30 days, butchers, and the weighing immune organ weight is calculated Immune Organs Index.
Table 5 faecium MDF1104 is on the impact of mink production performance
| Group | The diarrhoea number of elements | Dead number of elements | Average daily gain g | Average daily ingestion amount g | Immune Organs Index g |
| Control group | 11 | 4 | 22.28 | 368.81 | 0.36 |
| MDF1104-10 9cfu | 4 | 1 | 23.33 | 384.38 | 0.40 |
As shown in Table 5, in finishing period mink daily ration, add faecium MDF1104, the sickness rate of the seasonal diarrhoea of mink during can being reduced at the beginning of 8 the end of month to 9 month, compare with control group, diarrhea rate descends 40%, and mortality ratio descends 10%, can obviously improve the mink daily ingestion amount more than 4%, it is about 5% that average daily gain speed increases, and Immune Organs Index slightly increases.
Conclusion
The above results shows, faecium MDF1104 involved in the present invention, can increase daily ingestion amount and the day weight gain of mouse and mink, strengthen the immunity function of animal, faecium MDF1104 use separately or with conventional carrier combinations, can prevent the generation of the seasonal diarrhoea of mink, improve the production performance of animal.
Claims (3)
1. a faecium (Enterococcus faecium) MDF1104 preserves numbering CGMCC No.4237.
2. faecium MDF1104 claimed in claim 1 is improving the mink daily ingestion amount, the application of day weight gain aspect.
3. feed pre-mixing material, feed pre-mixing material contains the described faecium MDF1104 of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105696124A CN102093967B (en) | 2010-12-02 | 2010-12-02 | Mink source enterococcus faecium and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105696124A CN102093967B (en) | 2010-12-02 | 2010-12-02 | Mink source enterococcus faecium and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102093967A CN102093967A (en) | 2011-06-15 |
| CN102093967B true CN102093967B (en) | 2013-01-30 |
Family
ID=44127234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105696124A Expired - Fee Related CN102093967B (en) | 2010-12-02 | 2010-12-02 | Mink source enterococcus faecium and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102093967B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10322151B2 (en) | 2015-06-15 | 2019-06-18 | 4D Pharma Research Limited | Compositions comprising bacterial strains |
| US11779613B2 (en) | 2017-06-14 | 2023-10-10 | Cj Bioscience, Inc. | Compositions comprising a bacterial strain of the genus Megasphera and uses thereof |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201112091D0 (en) | 2011-07-14 | 2011-08-31 | Gt Biolog Ltd | Bacterial strains isolated from pigs |
| GB201117313D0 (en) | 2011-10-07 | 2011-11-16 | Gt Biolog Ltd | Bacterium for use in medicine |
| GB201306536D0 (en) | 2013-04-10 | 2013-05-22 | Gt Biolog Ltd | Polypeptide and immune modulation |
| SI3065748T1 (en) | 2014-12-23 | 2018-05-31 | 4D Pharma Research Limited | A bacteroides thetaiotaomicron strain and its use in reducing inflammation |
| KR20170091157A (en) | 2014-12-23 | 2017-08-08 | 4디 파마 리서치 리미티드 | Pirin polypeptide and immune modulation |
| CN104862254B (en) * | 2015-05-26 | 2018-02-16 | 北京好实沃生物技术有限公司 | One Enterococcus faecalis HEW A588 and its application |
| MA41060B1 (en) | 2015-06-15 | 2019-11-29 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
| MA41010B1 (en) | 2015-06-15 | 2020-01-31 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
| KR20180012849A (en) | 2015-06-15 | 2018-02-06 | 4디 파마 리서치 리미티드 | Composition Containing Bacterial Strain |
| ES2742514T3 (en) | 2015-06-15 | 2020-02-14 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
| PT3209310T (en) | 2015-11-20 | 2018-04-20 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
| GB201520497D0 (en) | 2015-11-20 | 2016-01-06 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
| GB201520631D0 (en) | 2015-11-23 | 2016-01-06 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
| GB201520638D0 (en) | 2015-11-23 | 2016-01-06 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
| CN105733977B (en) * | 2016-01-27 | 2019-06-14 | 南昌大学 | Probiotic feed additive |
| CN105733978B (en) * | 2016-01-27 | 2019-04-12 | 南昌大学 | One Enterococcus faecalis WEFA23 |
| CA3016179C (en) | 2016-03-04 | 2019-08-13 | 4D Pharma Plc | Compositions comprising bacterial blautia strains for treating visceral hypersensitivity |
| GB201612191D0 (en) | 2016-07-13 | 2016-08-24 | 4D Pharma Plc | Compositions comprising bacterial strains |
| TWI802545B (en) | 2016-07-13 | 2023-05-21 | 英商４Ｄ製藥有限公司 | Compositions comprising bacterial strains |
| GB201621123D0 (en) | 2016-12-12 | 2017-01-25 | 4D Pharma Plc | Compositions comprising bacterial strains |
| EP3630137B1 (en) | 2017-05-22 | 2023-05-17 | 4D Pharma Research Limited | Compositions comprising bacterial strains |
| MA41708A (en) | 2017-05-24 | 2020-04-08 | 4D Pharma Res Ltd | COMPOSITIONS CONTAINING BACTERIAL STRAINS |
| ES2855701T3 (en) | 2017-06-14 | 2021-09-24 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
| ES2841902T3 (en) | 2017-06-14 | 2021-07-12 | 4D Pharma Res Ltd | Compositions comprising bacterial strains |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005026269A1 (en) * | 2003-09-04 | 2005-03-24 | Mcdaniel C Steven | Microorganism coating components, coatings, and coated surfaces |
| CN101531986A (en) * | 2008-07-23 | 2009-09-16 | 中国农业科学院特产研究所 | Recombinant strain of mink IFN-Gamma gene |
| CN101601442A (en) * | 2008-11-05 | 2009-12-16 | 中国农业科学院特产研究所 | 1% mink compound premix special |
-
2010
- 2010-12-02 CN CN2010105696124A patent/CN102093967B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005026269A1 (en) * | 2003-09-04 | 2005-03-24 | Mcdaniel C Steven | Microorganism coating components, coatings, and coated surfaces |
| CN101531986A (en) * | 2008-07-23 | 2009-09-16 | 中国农业科学院特产研究所 | Recombinant strain of mink IFN-Gamma gene |
| CN101601442A (en) * | 2008-11-05 | 2009-12-16 | 中国农业科学院特产研究所 | 1% mink compound premix special |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10322151B2 (en) | 2015-06-15 | 2019-06-18 | 4D Pharma Research Limited | Compositions comprising bacterial strains |
| US11779613B2 (en) | 2017-06-14 | 2023-10-10 | Cj Bioscience, Inc. | Compositions comprising a bacterial strain of the genus Megasphera and uses thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102093967A (en) | 2011-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102093967B (en) | Mink source enterococcus faecium and application thereof | |
| CN101671638B (en) | New strain of bifidobacterium and fermentative preparation method and application thereof | |
| CN102876614B (en) | Bacillus licheniformis and application of bacillus licheniformis | |
| CN106260540B (en) | Biological feed for creep feed and creep feed | |
| CN102399733B (en) | Lactobacillus johnsonii, microbial inoculum, application and premix thereof | |
| CN102356816B (en) | Animal health care probiotics leavening capable of improving meat quality and application thereof | |
| CN107047934A (en) | Using bacillus subtilis strain to strengthen the method for animal health | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN102226162A (en) | Preparation method and application of composite microbial feed additive | |
| CN104293696A (en) | Enterococcus faecalis HEW-A131 and application thereof | |
| CN104862254A (en) | Enterococcus faecium HEW-A588 and application thereof | |
| CN115094012B (en) | Preparation method and application of bacillus coagulans BC-HYC strain microbial inoculum | |
| CN102391962B (en) | Enterococcus faecium preparation agent and manufacturing technology thereof | |
| CN101974463A (en) | Lactobacillus reuteri and composite viable bacteria preparation thereof | |
| CN105368755A (en) | Acid-yielding Enterococcus faecium, bacteriostatic microecological preparation and application thereof | |
| CN103289910A (en) | Solid fermentation production method of bacillus coagulans | |
| CN104673726A (en) | Porcine lactobacillus acidophilus freeze-drying preparation and application thereof | |
| CN107312732A (en) | A kind of probiotic feed additive | |
| CN101392223A (en) | Breeding method of microbial feed additive strain | |
| CN101971920A (en) | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof | |
| CN105132321A (en) | Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method | |
| CN102210454B (en) | Microbial compound preparation and preparation method thereof | |
| CN102907585A (en) | Probiotics and vitamin premix and mixture for middle pig | |
| CN105199978A (en) | Bacillus coagulans preparation for livestock breeding and preparation method thereof | |
| CN103550401A (en) | Method for preparing composite microbial ecological agent effervescent tablet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 130112 Jilin province Changchun Jingyue Economic Development Zone Industry Street No. 4899 Patentee after: INSTITUTE OF SPECIAL ANIMAL AND PLANT SCIENCES OF CAAS Address before: 132109 Jilin Province, Jilin city Changyi district left town Luming Street No. 15 Patentee before: INSTITUTE OF SPECIAL ANIMAL AND PLANT SCIENCES OF CAAS |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200821 Address after: No.860, Weijia village, laodafang Town, Liaozhong District, Shenyang City, Liaoning Province, 110200 Patentee after: SHENYANG BOYANG FEED Co.,Ltd. Address before: 130112 Jilin province Changchun Jingyue Economic Development Zone Industry Street No. 4899 Patentee before: INSTITUTE OF SPECIAL ANIMAL AND PLANT SCIENCES OF CAAS |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130130 |