CN102078799B - Preparation method of bacteria-bran composite granular material used for adsorbing metal ion - Google Patents
Preparation method of bacteria-bran composite granular material used for adsorbing metal ion Download PDFInfo
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- CN102078799B CN102078799B CN 201010244019 CN201010244019A CN102078799B CN 102078799 B CN102078799 B CN 102078799B CN 201010244019 CN201010244019 CN 201010244019 CN 201010244019 A CN201010244019 A CN 201010244019A CN 102078799 B CN102078799 B CN 102078799B
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Abstract
The invention relates to a method for preparing a metal ion adsorbing material in a water body, in particular for preparing bacteria liquid of composite bacteria by utilizing an improved JFM II culture medium and then preparing a bacteria-bran composite granular material. The preparation method is characterized in that (1) the improved JFM II culture medium comprises the following components of 0.5g of NH4NO3, 0.5g of NaNO3, 0.5g of K2HPO4, 0.5g of MgSO4.7H2O, 0.2g of CaCl2, 2g of citric acid, 8g of trisodium citrate and 0.013g of ammonium ferric citrate; and (2) bran is sterilized at high temperature; the bacteria liquid of the composite bacteria is added to be primarily and uniformly mixed; then carboxymethyl cellulose is added to be stirred; pelletizing is carried out in a pelletizer; and a mixture is naturally dried to prepare the bacteria-bran composite granular material. In the invention, the bran with favorable adsorbing effect and rich nutrient components is utilized as a supplementary material of the composite granular material, has the advantages of large specific surface area, favorable water retention and moisture retention and is easily stored; because the CMC (Carboxymethyl Cellulose ) is adopted, no biohazard or secondary pollution is generated; and the prepared granular material has favorable ferro-manganese removing capacity, is used for biological treatment on high concentration ferrimanganic ions in ground water in water treatment filtering and comes up to the corresponding emission standard.
Description
Technical field
The present invention relates to the preparation method of metal ion adsorption material in a kind of water body, is bacterium, wheat bran to be carried out compound specifically, is prepared into the preparation method of particulate material.Prepared particulate material is mainly used in the processing of underground water high concentration ferromanganese ion, reduces the ferromanganese ion concentration in the underground water, reaches corresponding discharge standard.
Background technology
Along with application and the development of microbial technique, increasing function bacterium be it is found that utilization.Has certain special ability from the bacterium of particular locality separation and purification, often the high metal of this mining area content is had higher resistance such as the microorganism that in a certain mining area, survives, can be by certain mechanism accumulation or by the special metal of chemical action precipitation.In addition, at occurring in nature, restrict each other between the microorganism, interact, may relate between the different bacterium take the synergy of common metabolism as the basis, mutually provide growth factor and energy, degraded the other side metabolic antagonist, balance pH value, keep suitable oxidation-reduction potential, elimination intermediate product etc.Many results of study show that the adaptive capacity of Mixed Microbes is strong than single culture, and the complementation between Mixed Microbes makes oxidation stronger than single bacterium.Although every kind of bacterium has certain growth rhythm, generally speaking, when bacterium and other bacterium symbiosis, it is a lot of than its fashion of growing separately to grow, and the growth adaptation phase is also shorter.The biological deferrization demanganization filtering layer itself is exactly the microecosystem of a complexity take the ferrimanganic bacterium as core, the at present research about the biological iron-removal from ground water demanganization mainly is conceived to single kind of microorganism, seldom the synergy that may exist between bacterium that common function is arranged and the bacterium is kept a close eye on.
As the wheat bran of agricultural product accessory substance, wide material sources, cheap and easy to get, contain the organic principles such as abundant crude fibre, lignin and crude protein, the nutrition that can be used as the bacterial reproduction growth provides.Simultaneously, owing to also contain a large amount of hydroxyls, carboxyl and some aldehyde, ketone, alcohol, ester on the cell membrane of wheat bran, complexing can occur with the surface of metal ion in these functional groups, in conjunction with heavy metal ion.Wheat bran is used as the existing application of metal biosorption material, but with wheat bran and the compound preparation granular materials of microorganism, utilizes its nutrition and absorption property, and the research that is used for underground water metal biosorption aspect yet there are no report.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of bacterium that can efficiently reduce ferromanganese ion concentration in the underground water-wheat bran composite particles material.
For realizing that the technical scheme that purpose of the present invention adopts is:
1, the preparation of compound bacteria bacterium liquid
JFM II culture medium (1000mL) prescription of improvement:
NH
4NO
30.5g, NaNO
30.5g, K
2HPO
40.5g, MgSO
47H
2O 0.5g, CaCl
20.2g, citric acid 2g, trisodium citrate 8g, ferric citrate 0.013g, after the dissolving, it is for subsequent use to regulate 6.8～7.2,121 ℃ of sterilizations of pH 20min.
Respectively pseudomonad (Pseudomonas sp.) and acinetobacter calcoaceticus (Acinetobacter sp.) slant culture are moved and connect 1 and encircle in the seed bottle, in 26 ℃, 130r/min cultivates 24h, obtain the exponential phase seed liquor, seed liquor be inoculated into enlarge in the JFMII culture medium of improvement cultivate 40h after, pseudomonad bacterium liquid and acinetobacter calcoaceticus bacterium liquid are concentrated into bacterial concentration OD
600nmAbout 2.0, in the ratio preparation composite bacterial bacterium liquid of volume (L) than 1: 3 of pseudomonad bacterium liquid and acinetobacter calcoaceticus bacterium liquid.
2, the preparation of bacterium-wheat bran composite particles material
With the wheat bran high-temperature sterilization; in the ratio that every kg wheat bran adds 30ml～65ml composite bacterial bacterium liquid, add composite bacterial bacterium liquid, preliminary mixing; add again the ratio adding carboxymethyl cellulose (CMC) of 0.045kg～0.065kg in every kg wheat bran; stir, make liquid and solid-state fine powder closely and fully contact granulator granulation; according to the requirement of using; particle diameter is controlled between 1.5mm～2.5mm, and natural air drying namely is prepared into bacterium-wheat bran composite particles material.
It is composite particles material auxiliary material that the present invention adopts advantages of good adsorption effect and the abundant wheat bran of nutritional labeling, and specific area is large, and the water conservation moisture retention is good, easily preserves; Select pseudomonad (Pseudomonas sp) and acinetobacter calcoaceticus (Acinetobacter sp.) to mix, bring into play its synergy; The CMC inanimate object is poisoned, and does not produce secondary pollution, the preparation process mild condition, and CMC has improved the mechanical strength of granular materials, and the material of preparing has preferably biocompatibility and certain mechanical anti-pressure ability.The main purposes of this granular materials is to have good ferrimanganic to remove ability, is used for the biological treating of the ferromanganese ion in the Water treatment filtration underground water.In addition, in the engineering of water treatment metal ion is used, usually filter post and reach the maturity period, namely can need at least 2～3 months by the steady decrease concentration of metal ions, this particulate material can reduce the maturity period of filter post, makes the maturity period foreshorten to 1 month.
The specific embodiment
Embodiment 1
1, the preparation of compound bacteria bacterium liquid
Raw material: pseudomonad (Pseudomonas sp.), acinetobacter calcoaceticus (Acinetobacter sp.) is available from Chinese industrial microorganism fungus kind preservation administrative center; Wheat bran is available from Minhou County Jianping, Foochow, Fujian village.
The JFMII culture medium (1000mL) of preparation improvement: NH
4NO
31.0g, NaNO
31.0g, K
2HPO
41.0g, MgSO
47H
2O 1.0g, CaCl
20.4g, citric acid 4.0g, trisodium citrate 8.0g, ferric citrate 0.026g after being dissolved in water, regulates pH to 7.2,121 ℃ of sterilization 20min.
(1) will have respectively pseudomonad and acinetobacter calcoaceticus slant culture and move and connect 1 and encircle in the seed bottle, in 26 ℃, 130r/min cultivates 24h, obtains the exponential phase seed liquor, and seed liquor is inoculated into respectively in the 5 liter culture tank again and enlarges cultivation.After cultivating 40h, be concentrated into bacterial concentration OD with enlarging pseudomonad and the acinetobacter calcoaceticus bacterium liquid cultivated
600nmAbout 2.0, in the ratio preparation composite bacterial bacterium liquid of volume (L) than 1: 3 of pseudomonad bacterium liquid and acinetobacter calcoaceticus bacterium liquid.
2, the preparation of bacterium-wheat bran composite particles material
The wheat bran of getting 10kg carries out high-temperature sterilization, in the ratio that every kg wheat bran adds 30ml composite bacterial bacterium liquid, adds composite bacterial bacterium liquid 300ml, mixing.Add again the CMC of the ratio adding 0.6kg of 0.06kg in every kg wheat bran, stir, make the close and abundant mixing of liquid and solid-state fine powder, stirring-granulating, particle diameter is controlled at 1.5mm, and natural air drying namely is prepared into bacterium-wheat bran composite particles material.
The bacterium of above-mentioned preparation-wheat bran composite particles material is packed in the shaking flask of 50mL finite concentration ferromanganese ion solution, in 26 ℃, the 130r/min shaking table is cultivated, behind the 12h, the concentration that detects iron ion drops to 6.68mg/L by the 22.89mg/L before processing, the concentration of manganese ion drops to 0.37mg/L by the 0.88mg/L before processing, and clearance is respectively 70.82% and 57.95%.
Embodiment 2
1, the preparation of compound bacteria bacterium liquid
Raw material: with embodiment 1.
The JFM II culture medium (1000mL) of preparation improvement: NH
4NO
31.0g, NaNO
31.0g, K
2HPO
41.0g, MgSO
47H
2O 1.0g, CaCl
20.4g, citric acid 4.0g, trisodium citrate 8.0g, ferric citrate 0.026g after being dissolved in water, regulates pH to 7.2,121 ℃ of sterilization 20min.
(1) respectively pseudomonad and acinetobacter calcoaceticus slant culture are moved and connect 1 and encircle in the seed bottle, in 26 ℃, 130r/min cultivates 24h, obtains the exponential phase seed liquor, and seed liquor is inoculated into respectively in the 5 liter culture tank again and enlarges cultivation.After cultivating 40h, be concentrated into bacterial concentration OD with enlarging pseudomonad and the acinetobacter calcoaceticus bacterium liquid cultivated
600nmAbout 2.0, in the ratio preparation composite bacterial bacterium liquid of volume (L) than 1: 3 of pseudomonad bacterium liquid and acinetobacter calcoaceticus bacterium liquid.
2, the preparation of bacterium-wheat bran composite particles material
The wheat bran of getting 10kg carries out high-temperature sterilization, add the ratio of 30ml composite bacterial bacterium liquid in every kg wheat bran, add composite bacterial bacterium liquid 300ml, preliminary mixing adds the carboxyl methyl cellulose of the ratio adding 0.5kg of 0.05kg again by every kg wheat bran, stir, make the close and abundant mixing of liquid and solid-state fine powder, stirring-granulating, particle diameter is controlled at 2.0mm, natural air drying namely is prepared into bacterium-wheat bran composite particles material.
The bacterium of above-mentioned preparation-wheat bran composite particles material is packed in the shaking flask of 50mL finite concentration ferromanganese ion solution, in 26 ℃, the 130r/min shaking table is cultivated, behind the 24h, the concentration that detects iron ion drops to 3.12mg/L by the 22.89mg/L before processing, the concentration of manganese ion drops to 0.32mg/L by 0.88 before processing, and clearance is respectively 86.37% and 63.64%.
Embodiment 3
1, the preparation of compound bacteria bacterium liquid
Raw material: with embodiment 1.
The JFM II culture medium (1000mL) of preparation improvement: NH
4NO
31.0g, NaNO
31.0g, K
2HPO
41.0g, MgSO
47H
2O 1.0g, CaCl
20.4g, citric acid 4.0g, trisodium citrate 8.0g, ferric citrate 0.026g after being dissolved in water, regulates pH to 7.2,121 ℃ of sterilization 20min.
(1) respectively pseudomonad and acinetobacter calcoaceticus slant culture are moved and connect 1 and encircle in the seed bottle, in 26 ℃, 130r/min cultivates 24h, obtains the exponential phase seed liquor, and seed liquor is inoculated into respectively in the 5 liter culture tank again and enlarges cultivation.After cultivating 40h, be concentrated into bacterial concentration OD with enlarging pseudomonad and the acinetobacter calcoaceticus bacterium liquid cultivated
600nmAbout 2.0, in the ratio preparation composite bacterial bacterium liquid of volume (L) than 1: 3 of pseudomonad bacterium liquid and acinetobacter calcoaceticus bacterium liquid.
2, the preparation of the preparation composite bacteria agent capable granular materials of bacterium-wheat bran composite particles material
The wheat bran of getting 10kg carries out high-temperature sterilization, add the ratio of 50ml composite bacterial bacterium liquid in every kg wheat bran, add composite bacterial bacterium liquid 500ml, preliminary mixing adds the CMC of the ratio adding 0.45kg of 0.045kg again by every kg wheat bran, stir, make the close and abundant mixing of liquid and solid-state fine powder, stirring-granulating, particle diameter is controlled at 2.5mm, natural air drying namely is prepared into bacterium-wheat bran composite particles material.
The bacterium of above-mentioned preparation-wheat bran composite particles material is packed in the glass column, with the flow of 110ml/h the underground water that is rich in ferrimanganic is carried out filtration treatment.After the suction-operated of filtrate reaches capacity the 30th day, after testing, the concentration of iron ion drops to 4.27mg/L by the 98.73mg/L before processing, and the concentration of manganese ion drops to 2.19mg/L by the 38.12mg/L before processing, and clearance is respectively 95.68% and 94.25%.
Claims (4)
1. the preparation method of the bacterium for adsorbing metal ions-wheat bran composite particles material at first is the JFM II culture medium that utilizes improvement, and the bacterium liquid of preparation compound bacteria then is prepared into bacterium-wheat bran composite particles material again, it is characterized in that
1) the JFM II culture medium of improvement, in 1000mL, its prescription:
NH
4NO
30.5g, NaNO
30.5g, K
2HPO
40.5g, MgSO
47H
2O 0.5g, CaCl
20.2g, citric acid 2g, trisodium citrate 8g, ferric citrate 0.013g; PH 6.8～7.2,121 ℃ of sterilizations;
2) preparation of compound bacteria bacterium liquid
Respectively pseudomonad and acinetobacter calcoaceticus slant culture are moved and connect 1 and encircle in the seed bottle, in 26 ℃, 130r/min cultivates 24h, obtain the exponential phase seed liquor, seed liquor be inoculated into enlarge in the JFMII culture medium of improvement cultivate 40h after, pseudomonad bacterium liquid and acinetobacter calcoaceticus bacterium liquid are concentrated into bacterial concentration OD
600nm2.0, in 1: 3 ratio of the volume ratio preparation composite bacterial bacterium liquid of pseudomonad bacterium liquid and acinetobacter calcoaceticus bacterium liquid;
3) preparation of bacterium-wheat bran composite particles material
With the wheat bran high-temperature sterilization, add composite bacterial bacterium liquid, preliminary mixing adds carboxymethyl cellulose again, stirs, and granulator granulation, natural air drying namely are prepared into bacterium-wheat bran composite particles material.
2. the preparation method of a kind of bacterium for adsorbing metal ions according to claim 1-wheat bran composite particles material is characterized in that the amount of the composite bacterial bacterium liquid that adds is that every kg wheat bran adds 30ml～65ml.
3. the preparation method of a kind of bacterium for adsorbing metal ions according to claim 1-wheat bran composite particles material is characterized in that the amount of the carboxymethyl cellulose that adds is that every kg wheat bran adds 0.045kg～0.065kg.
4. the preparation method of a kind of bacterium for adsorbing metal ions according to claim 1-wheat bran composite particles material is characterized in that described granulation, and its particle diameter is controlled between 1.5mm～2.5mm.
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| CN105618008B (en) * | 2016-01-20 | 2018-02-09 | 中南大学 | A kind of biological composite membrane material and its methods for making and using same |
| CN105521769B (en) * | 2016-01-20 | 2017-08-29 | 中南大学 | A kind of application process of biological composite membrane material |
| CN111675445A (en) * | 2016-07-20 | 2020-09-18 | 张哲夫 | Treatment method for removing cellulose in papermaking wastewater |
| JP2018196853A (en) * | 2017-05-23 | 2018-12-13 | 清水建設株式会社 | Elution suppression method of heavy metals and elution suppression material thereof |
| CN112427018A (en) * | 2020-11-03 | 2021-03-02 | 常州市和润环保科技有限公司 | Heavy metal wastewater biomass adsorbent and preparation method thereof |
| CN113080464B (en) * | 2021-04-15 | 2023-06-16 | 河南工业大学 | Preparation method of selenium-enriched material |
Citations (3)
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|---|---|---|---|---|
| US5569596A (en) * | 1995-01-04 | 1996-10-29 | The Board Of Regents Of The University Of Oklahoma | Method for bacterial reduction of chromium (VI) |
| CN1850636A (en) * | 2006-05-19 | 2006-10-25 | 东华大学 | Method for preparing bacterial cellulose suspension for efficient rapid heavy metal ion adsorption |
| CN1891642A (en) * | 2005-07-01 | 2007-01-10 | 中国辐射防护研究院 | Method for treating mercury and divalent metal ion-containing sewage by using pseudomonas cepacia |
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2010
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5569596A (en) * | 1995-01-04 | 1996-10-29 | The Board Of Regents Of The University Of Oklahoma | Method for bacterial reduction of chromium (VI) |
| CN1891642A (en) * | 2005-07-01 | 2007-01-10 | 中国辐射防护研究院 | Method for treating mercury and divalent metal ion-containing sewage by using pseudomonas cepacia |
| CN1850636A (en) * | 2006-05-19 | 2006-10-25 | 东华大学 | Method for preparing bacterial cellulose suspension for efficient rapid heavy metal ion adsorption |
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