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CN101983062A - Abca-1 elevating compounds and the use thereof - Google Patents

Abca-1 elevating compounds and the use thereof Download PDF

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CN101983062A
CN101983062A CN200980111878XA CN200980111878A CN101983062A CN 101983062 A CN101983062 A CN 101983062A CN 200980111878X A CN200980111878X A CN 200980111878XA CN 200980111878 A CN200980111878 A CN 200980111878A CN 101983062 A CN101983062 A CN 101983062A
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optional replacement
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阿尔温德·达尔拉
杰弗里·奇泽姆
路易斯·贝拉尔迪内利
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Gilead Palo Alto Inc
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CV Therapeutics Inc
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Abstract

Disclosed are novel compounds of Formula (1) useful for treating various disease states, in particular, insulin resistance, diabetes, dyslipidemia, coronary artery disease, and inflammation. The compounds of the present invention elevate cellular expression of the ABCA-1 gene as well as increasing the level of ABCA-1 protein, which may result in an increase in HDL levels in the plasma of a mammal, in particular humans.

Description

Improve chemical compound of ABCA-1 and uses thereof
The application requires in the U.S. Provisional Patent Application sequence No.61/027 of submission on February 7th, 2008,016 priority, and whole disclosures of this application are incorporated in this by reference.
Technical field
The present invention relates to the useful chemical compound of cell ABCA-1 output in the raising mammal, and relate to the method for in the treatment coronary artery disease, using such chemical compound.The present invention also relates to contain the pharmaceutical composition of such chemical compound.
Background technology
Cholesterol is that higher organism bulk-growth and survival are necessary.It is a lipid of regulating the eukaryotic cell membrane fluidity, and is the precursor of steroid hormone such as progesterone, testosterone etc.Cholesterol can obtain from diet, or can be inner synthetic in liver and intestinal.Cholesterol is transported to particular target by lipoprotein in body fluid, wherein lipoprotein can be classified according to the density that increases.For example, low-density lipoprotein cholesterol (LDL) is responsible for cholesterol transport to liver with transport out liver, and is transported to peripheral tissues's cell, and here ldl receptor is in conjunction with LDL, and mediates it and enter cell.
Although cholesterol is absolutely necessary to the many biological processes in the mammal, but the LDL cholesterol serum levels that raises do not expect because known they promote the formation of the atheromatous plaque in the whole health tremulous pulse, this can cause, for example, the development of coronary artery disease.On the contrary, based on people's clinical data and animal model system, found that HDL-C (HDL-C) level that raises can stop the development of coronary artery disease.
Usually, too much cholesterol is removed from health by the process that high density lipoprotein (HDL) participates in.Cholesterol by one in two processes from cell flow out-or by passive transfer to sophisticated HDL, or by initiatively transferring to aPoA-I.The process in back is by being called the protein mediated of ATP binding cassette transporter body 1 (ABC-1, or replacedly be called ABCA-1).In a back process, poor fat (lipid-poor) HDL precursor obtains phospholipid and cholesterol, and this causes the particulate blood plasma level of sophisticated HDL to increase.The HDL cholesterol finally is transported to liver in the process that is called " cholesterol antiport ", here it otherwise be recycled, or drained as bile.
Being intended to reduce a kind of Therapeutic Method that tremulous pulse medium-sized artery atherosclerotic plaque forms risk relates to and reduces the blood plasma lipide level.Such method comprises the change diet, and/or heal with medicine, medicine wherein is the derivant (clofibrate (clofibrate) of for example Carboxymethylcellulose, gemfibrozil (gemfibrozil) and fenofibrate (fenofibrate)), nicotinic acid and HMG-CoA reductase inhibitor, Mei Feinuolin (mevinolin) for example, mevastatin (mevastatin), pravastatin (pravastatin), simvastatin (simvastatin), fluvastatin (fluvastatin) and lovastatin (lovastatin), or they are by synthetic in the cell that suppresses cholesterol, or by suppressing to reduce blood plasma LDL cholesterol levels via the absorption of ldl receptor.In addition, the bile acid binding resin, for example cholestyramine (cholestyrine), colestipol (colestipol) and probucol (probucol) reduce the LDL-cholesterol level by the catabolism that reduces LDL-cholesterol in intestinal absorption and the increase liver.
Expectation provides and is intended to reduce the replacement therapy that forms the atheromatous plaque risk in the tremulous pulse, especially removes the individuality of cholesterol ability for lacking from arterial wall through the HDL approach.Suppose that the HDL level is general relevant with the expression of ABCA-1, increase the expression of a kind of method increase ABCA-1 of HDL level.Therefore, expectation provides such chemical compound, and it is the active stimulus that ABCA-1 expresses in the mammal, thereby increases the outflow of cholesterol and improve HDL cholesterol level in the blood.This will be that the treatment of various disease states of feature is useful, particularly useful for the treatment of coronary artery disease with the low HDL levels to treatment.
Should be noted that to show that the production that improves ABCA-1 in the macrophage can reduce the deposition of cholesterol in the coronary artery in the part, and does not significantly improve blood plasma HDL cholesterol.In this case, even under the situation that does not increase the HDL cholesterol, it also is favourable improving the ABCA-1 expression.
Summary of the invention
Therefore, representative instance of the present invention as described in this article provides such chemical compound, it improves the cellular expression of ABCA-1 gene and/or improves the ABCA-1 protein expression, thereby increases the level of blood plasma middle-high density lipoprotein cholesterol (HDL-C) and reduce lipid level in the mammal.In concrete embodiment, the present invention relates to the chemical compound of formula I:
Figure BPA00001233335000031
Formula I
Wherein:
R is a hydrogen;
R 1Be the aryl of the cycloalkyl of the alkyl of optional replacement, optional replacement, optional replacement or the heteroaryl of optional replacement; Or
R and YR 1Represent the heterocyclic radical of optional replacement with the nitrogen-atoms that links to each other with them;
R 2Be hydrogen, halogen, trifluoromethyl, acyl group, or cyano group;
R 3Be the aryl of the cycloalkyl of optional replacement, optional replacement, the heteroaryl of optional replacement, or the heterocyclic radical of optional replacement;
R 4And R 5Be hydrogen or acyl group independently; And
X and Y are the alkylidene of covalent bond or optional replacement independently; Prerequisite is to work as R 1Be methyl and Y when being covalent bond, when X is methylene or ethylidene, R 3Can not be phenyl.
In some embodiments, the present invention relates to a kind of formula I compounds for treating that uses can and/or improve the effective mammiferous disease for the treatment of of chemical compound of ABCA-1 protein expression or the method for disease with the cellular expression that improves the ABCA-1 gene, and this method comprises the formula I chemical compound for the treatment of effective dose to its mammal of needs.Such disease includes, but are not limited to, arterial disease, particularly coronary artery disease.In some embodiments, the feature of this disease is low-level HDL cholesterol.In some embodiments, this disease or disease can be one or more in diabetes, insulin resistant, dyslipidemia, coronary artery disease and the inflammation.
In typical embodiment, the method according to this invention comprises that use formula I compounds for treating can and/or improve the proteic expression of ABCA-1 and also improve the chemical compound effectively mammiferous disease of treatment or the method for disease of the serum levels of HDL cholesterol with the cellular expression that improves the ABCA-1 gene, and this method comprises the formula I chemical compound for the treatment of effective dose to its mammal of needs.Such disease includes, but are not limited to, arterial disease, particularly coronary artery disease.In some embodiments, the feature of this disease is low-level HDL cholesterol.In some embodiments, this disease or disease can be one or more in diabetes, insulin resistant, dyslipidemia, coronary artery disease and the inflammation.
The present invention, embodiment particularly, relate to the cellular expression that is used for the treatment of available raising ABCA-1 gene and/or improve the effective mammiferous disease for the treatment of of chemical compound of ABCA-1 protein expression or the pharmaceutical preparation of disease, it comprises the formula I chemical compound and at least a pharmaceutically acceptable excipient for the treatment of effective dose.
In some embodiments, the present invention relates to the method for preparation I compound.
In some embodiments of the present invention, R 3Be the aryl of optional replacement or the heteroaryl of optional replacement, especially at R, R 2, R 4And R 5Complete is under the situation of hydrogen.
In some embodiments, R 3Be the aryl (for example, the phenyl of optional replacement) of optional replacement, R 1Be the cycloalkyl of optional replacement, X is a covalent bond.In some such embodiments, R 3Be halogen, the phenyl that replaces of fluorine especially, and R 1Be the cyclopenta of optional replacement, 2-hydroxycyclopent base especially.
In other embodiments, R 1And R 3All be the phenyl of optional replacement, X is a covalent bond, and Y is the low-grade alkylidene of optional replacement, and especially wherein Y is those chemical compounds of ethylidene, propylidene or the propylidene that replaced by phenyl.
In other embodiments, R 1Be the alkyl of optional replacement or the phenyl of optional replacement, R 3Be the phenyl of optional replacement, and X and Y are covalent bonds.In some such embodiments, R 1Be low alkyl group or 2-fluorophenyl, and R 3Be phenyl or 2-fluorophenyl.
In other embodiments, R 3Be the heteroaryl of optional replacement, for example, 1 of the 1,3-thiazoles of optional replacement-2-base or optional replacement, 3-benzoxazole-2-base.In some such embodiments, R 1Be the cycloalkyl of optional replacement or the phenyl of optional replacement, X is a covalent bond, and Y is covalent bond or alkylidene.In some embodiments, R 1Be bicyclic alkyl, dicyclo [2.2.1] heptan-2-base particularly, and Y is a covalent bond.In some embodiments, R 1Be monocyclic, cyclopropyl especially, and Y is a methylene.In some other embodiments, R 1Be phenyl, and Y is a low-grade alkylidene.
In some embodiments, R 2, R 4And R 5All be hydrogen, and R and YR 1Represent nitrogen heterocycle with the nitrogen that links to each other with them.Some such embodiment comprises such chemical compound, wherein R 3Be the phenyl of optional replacement or the heteroaryl of optional replacement, and X is a covalent bond, especially R and YR 1Represent pyrrolin-1-base with the nitrogen that links to each other with them.
Description of drawings
Fig. 1 shows the time-histories (time-cource) of processing to the influence of the ABCA1 gene expression in the liver of ZDF (Zucker diabetes obesity) rat.Handled rat at 0,2 and 4 hour with the test compound of formula I.With the rat of handling significant difference is arranged with excipient *) p＜0.05, *) p＜0.01[ *Variation 3619 in figure and the experiment *].
Fig. 2 shows the time-histories of processing to the influence of the liver ABCA1 protein expression in the ZDF rat.Handled rat at 0,2 and 4 hour with the test compound of formula I.Along with the time carries out, handle the protein expression that increases ABCA1.With the rat of handling significant difference is arranged with excipient *) p＜0.05, *) p＜0.01.The ABCA1 protein expression detects by Western blotting and is undertaken quantitatively by optical densitometric method, puts excipient service time and contrasts the ABCA1 to each time point to express standardization.
The specific embodiment
Definition and general parameter
As using in this manual, the implication that provides below is provided usually for following term and phrase, unless use their context to indicate in addition.
Term " alkyl " refers to the branching with 1～20 carbon atom or the monoradical of branching saturated hydrocarbon chain not.The group that this term can exemplify is, for example methyl, ethyl, n-pro-pyl, isopropyl, normal-butyl, isobutyl group, the tert-butyl group, n-hexyl, positive decyl, myristyl etc.
Term " alkyl of replacement " refers to:
1) has 1～5 substituent group; preferred 1～3 substituent alkyl as defined above, substituent group wherein is selected from the group of being made up of following group: thiazolinyl; alkynyl; alkoxyl; cycloalkyl; cycloalkenyl group; acyl group; acylamino-; acyloxy; amino; aminocarbonyl; alkoxycarbonyl amino; azido; cyano group; halogen; hydroxyl; ketone group; thiocarbonyl; carboxyl; carboxyalkyl; arylthio; heteroarylthio; the heterocyclic radical sulfenyl; mercaptan; alkylthio group; aryl; aryloxy group; heteroaryl; amino-sulfonyl; amino carbonyl amino; heteroaryl oxygen base; heterocyclic radical; the heterocyclyloxy base; hydroxylamino; alkoxy amino; nitro;-SO-alkyl;-SO-aryl;-SO-heteroaryl;-SO 2-alkyl ,-SO 2-aryl and-SO 2-heteroaryl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1～3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nThe substituent group of R (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces; Or
2) independently be selected from oxygen, sulfur and-NR a-1～5 atom or the alkyl as defined above that interrupts of group, wherein R aBe selected from hydrogen, alkyl, cycloalkyl, thiazolinyl, cycloalkenyl group, alkynyl, aryl, heteroaryl and heterocyclic radical.All substituent groups can be alternatively by alkyl, alkoxyl, halogen, CF 3, amino, the amino, the cyano group that replace, or-S (O) nR (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) further replaces; Or
3) has 1～5 substituent group but also by the 1-5 alkyl as defined above that interrupts of atom or group as defined above as defined above.
Term " low alkyl group " refers to the branching with 1～6 carbon atom or the monoradical of branching saturated hydrocarbon chain not.The group that this term can exemplify is, for example methyl, ethyl, n-pro-pyl, isopropyl, normal-butyl, isobutyl group, the tert-butyl group, n-hexyl etc.
Term " low alkyl group of replacement " refers to has 1～5 substituent group, the low alkyl group as defined above of preferred 1～3 substituent group (as what the alkyl that replaces was limited), perhaps by the low alkyl group as defined above of 1～5 atom (as what the alkyl that replaces was limited) interruption, perhaps has as defined above 1～5 substituent group but also by 1～5 low alkyl group as defined above that atom interrupts as defined above.
Term " alkylidene " refers to the branching or the divalent group of branching saturated hydrocarbon chain not, preferably has 1～20 carbon atom, preferably has 1～10 carbon atom, more preferably has 1～6 carbon atom.The group that this term can exemplify is, for example, and methylene (CH 2-), ethylidene (CH 2CH 2-), the propylidene isomer (for example ,-CH 2CH 2CH 2-and-CH (CH 3) CH 2-) etc.
Term " low-grade alkylidene " refers to the branching with 1～6 carbon atom or two bases (diradical) of branching saturated hydrocarbon chain not.
Term " alkylidene of replacement " refers to:
1) have 1～5 substituent alkylidene as defined above, substituent group wherein is selected from the group of being made up of following group: alkyl; thiazolinyl; alkynyl; alkoxyl; cycloalkyl; cycloalkenyl group; acyl group; acylamino-; acyloxy; amino; aminocarbonyl; alkoxycarbonyl amino; azido; cyano group; halogen; hydroxyl; ketone group; thiocarbonyl; carboxyl; carboxyalkyl; arylthio; the heteroaryl sulfenyl; the heterocyclic radical sulfenyl; mercaptan; alkylthio group; aryl; aryloxy group; heteroaryl; amino-sulfonyl; amino carbonyl amino; heteroaryl oxygen base; heterocyclic radical; the heterocyclyloxy base; hydroxylamino; alkoxy amino; nitro;-SO-alkyl;-SO-aryl;-SO-heteroaryl;-SO 2-alkyl ,-SO 2-aryl and-SO 2-heteroaryl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1～3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nThe substituent group of R (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces; Or
2) independently be selected from oxygen, sulfur and-NR a-1～5 atom or group or be selected from the alkylidene as defined above that the group of carbonyl, carboxylate, Carboxylamide and sulfonyl interrupts, wherein R aBe selected from alkyl, cycloalkyl, cycloalkenyl group, aryl, heteroaryl and the heterocyclic radical of hydrogen, optional replacement; Or
3) has as defined above 1～5 substituent group but also by the alkylidene as defined above of 1-20 atom interruption as defined above.The example of the alkylidene that replaces is chloro methylene (CH (Cl)-), amino ethylidene (CH (NH 2) CH 2-), methylamino ethylidene (CH (NHMe) CH 2-), 2-carboxyl propylidene isomer (CH 2CH (CO 2H) CH 2-), ethoxyethyl group (CH 2CH 2O-CH 2CH 2-), ethyl methylamino ethyl (CH 2CH 2N (CH 3) CH 2CH 2-), 1-ethyoxyl-2-(2-ethyoxyl-ethyoxyl) ethane (CH 2CH 2O-CH 2CH 2-OCH 2CH 2-OCH 2CH 2-) etc.
Term " aralkyl " refers to covalently bound aryl to alkylidene, and wherein aryl and alkylidene are to define in this article." aralkyl of optional replacement " refers to the covalently bound aryl that arrives the alkylidene of optional replacement.Such aralkyl can exemplified by benzyl, 3-(4-methoxyphenyl) propyl group etc.
Term " alkoxyl " refers to radicals R-O-, wherein R is the alkyl of optional replacement or the cycloalkyl of optional replacement, or R is-the Y-Z group, wherein Y is the alkylidene of optional replacement, and Z is the thiazolinyl of optional replacement, the alkynyl of optional replacement, or the cycloalkenyl group of optional replacement, wherein alkyl, thiazolinyl, alkynyl, cycloalkyl and cycloalkenyl group are as definition in this article.Preferred alkoxyl is alkyl-O-, and comprises for example methoxyl group, ethyoxyl, positive propoxy, isopropoxy, n-butoxy, tert-butoxy, sec-butoxy, n-pentyloxy, positive hexyloxy, 1,2-dimethyl butoxy etc.
Term " alkylthio group " refers to radicals R-S-, and wherein R is as defining alkoxyl.
Term " thiazolinyl " refers to the branching or the monoradical of branching unsaturated alkyl not, preferably has 2～20 carbon atoms, more preferably has 2～10 carbon atoms, even preferably has 2～6 carbon atoms, and have 1～6, preferred 1 two key (vinyl).Preferred thiazolinyl comprises vinyl (CH=CH 2), 1-acrylic or pi-allyl (CH 2CH=CH 2), isopropenyl (C (CH 3)=CH 2)), dicyclo [2.2.1] heptene etc.Under thiazolinyl and situation that nitrogen links to each other, two keys can not be in the α position of nitrogen.
Term " low-grade alkenyl " refers to the thiazolinyl as defined above with 2～6 carbon atoms.
Term " thiazolinyl of replacement " refers to has 1～5 substituent group; preferred 1～3 substituent thiazolinyl as defined above, substituent group wherein is selected from the group of being made up of following group: alkyl; thiazolinyl; alkynyl; alkoxyl; cycloalkyl; cycloalkenyl group; acyl group; acylamino-; acyloxy; amino; aminocarbonyl; alkoxycarbonyl amino; azido; cyano group; halogen; hydroxyl; ketone group; thiocarbonyl; carboxyl; carboxyalkyl; arylthio; the heteroaryl sulfenyl; the heterocyclic radical sulfenyl; mercaptan; alkylthio group; aryl; aryloxy group; heteroaryl; amino-sulfonyl; amino carbonyl amino; heteroaryl oxygen base; heterocyclic radical; the heterocyclyloxy base; hydroxylamino; alcoxyl amino; nitro;-SO-alkyl;-SO-aryl;-SO-heteroaryl;-SO 2-alkyl ,-SO 2-aryl and-SO 2-heteroaryl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1～3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nThe substituent group of R (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces.
Term " alkynyl " refers to the monoradical of unsaturated hydrocarbons, preferably has 2～20 carbon atoms, more preferably have 2～10 carbon atoms and even more preferably have 2～6 carbon atoms, and have at least 1, the preferred unsaturated position of 1-6 acetylene (triple bond).Preferred alkynyl comprises acetenyl (C ≡ CH), propargyl (or propinyl ,-C ≡ CCH 3) etc.Under alkynyl and situation that nitrogen-atoms links to each other, triple bond can not be in the α position of nitrogen-atoms.
Term " alkynyl of replacement " refers to has 1～5 substituent group; preferred 1～3 substituent alkynyl as defined above, substituent group wherein is selected from the group of being made up of following group: alkyl; thiazolinyl; alkynyl; alkoxyl; cycloalkyl; cycloalkenyl group; acyl group; acylamino-; acyloxy; amino; aminocarbonyl; alkoxycarbonyl amino; azido; cyano group; halogen; hydroxyl; ketone group; thiocarbonyl; carboxyl; carboxyalkyl; arylthio; the heteroaryl sulfenyl; the heterocyclic radical sulfenyl; mercaptan; alkylthio group; aryl; aryloxy group; heteroaryl; amino-sulfonyl; amino carbonyl amino; heteroaryl oxygen base; heterocyclic radical; the heterocyclyloxy base; hydroxylamino; alkoxy amino; nitro;-SO-alkyl;-SO-aryl;-SO-heteroaryl;-SO 2-alkyl ,-SO 2-aryl and-SO 2-heteroaryl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1～3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nThe substituent group of R (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces.
Term " aminocarbonyl " refers to group-C (O) NRR, and wherein each R is hydrogen, alkyl, aryl, heteroaryl, heterocyclic radical independently, or wherein two R groups connect and formation heterocyclic radical (for example, morpholino).All substituent groups can be alternatively further by alkyl, alkoxyl, halogen, CF 3, amino, the amino, the cyano group that replace, or-S (O) nR (wherein R is alkyl, aryl, or heteroaryl and n are 0,1 or 2) replaces.
Term " acylamino-" refers to group-NRC (O) R, and wherein each R can be hydrogen, alkyl, aryl, heteroaryl independently all, or heterocyclic radical.All substituent groups can be alternatively further by alkyl, alkoxyl, halogen, CF 3, amino, the amino, the cyano group that replace, or-S (O) nR (wherein R is alkyl, aryl, or heteroaryl and n are 0,1 or 2) replaces.
Term " acyloxy " refer to group-O (O) C-alkyl ,-O (O) C-cycloalkyl ,-O (O) C-aryl ,-O (O) C-heteroaryl and-O (O) C-heterocyclic radical.All substituent groups can be alternatively further by alkyl, alkoxyl, halogen, CF 3, amino, the amino, the cyano group that replace, or-S (O) nR (wherein R is alkyl, aryl, or heteroaryl and n are 0,1 or 2) replaces.
Term " aryl " refers to has monocycle (for example, phenyl) or multi-ring (for example, biphenylyl), or the aromatic carbocyclic group of 6～20 carbon atoms of a plurality of thick (condensing) ring (for example, naphthyl or anthryl).Preferred aryl groups comprises phenyl, naphthyl etc.
Unless to qualification is arranged in the definition of aryl substituent in addition; such aryl can be alternatively by 1～5 substituent group; preferred 1～3 substituent group replaces, and this substituent group is selected from the group of being made up of following group: alkyl; thiazolinyl; alkynyl; alkoxyl; cycloalkyl; cycloalkenyl group; acyl group; acylamino-; acyloxy; amino; aminocarbonyl; alkoxycarbonyl amino; azido; cyano group; halogen; hydroxyl; ketone group; thiocarbonyl; carboxyl; carboxyalkyl; arylthio; the heteroaryl sulfenyl; the heterocyclic radical sulfenyl; mercaptan; alkylthio group; aryl; aryloxy group; heteroaryl; amino-sulfonyl; amino carbonyl amino; heteroaryl oxygen base; heterocyclic radical; the heterocyclyloxy base; hydroxylamino; alcoxyl amino; nitro;-SO-alkyl;-SO-aryl;-SO-heteroaryl;-SO 2-alkyl ,-SO 2-aryl and-SO 2-heteroaryl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1～3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nThe substituent group of R (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces.
Term " aryloxy group " refers to group aryl-O-, and wherein aryl is as defined above, and comprises also the aryl of optional replacement as defined above.Term " arylthio " refers to radicals R-S-, and wherein R is as defined to aryl.
Term " amino " refers to group-NH 2
Term " amino of replacement " refers to group-NRR, wherein each R all is independently selected from the group of being made up of following group: hydrogen, alkyl, cycloalkyl, carboxyalkyl are (for example, benzyloxycarbonyl), aryl, heteroaryl and heterocyclic radical, prerequisite is that two R groups are not hydrogen simultaneously; Or-the Y-Z group, wherein Y is the alkylidene of optional replacement, and Z is thiazolinyl, cycloalkenyl group, or alkynyl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1～3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nR (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) substituent group replaces.
Term " carboxyalkyl " refer to group-C (O) O-alkyl ,-C (O) O-cycloalkyl, wherein alkyl and cycloalkyl be as defined in this article, and can be alternatively further by alkyl, thiazolinyl, alkynyl, alkoxyl, halogen, CF 3, amino, the amino, the cyano group that replace, or-S (O) nR (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces.
Term " cycloalkyl " refers to the cyclic alkyl of 3～20 carbon atoms with single cyclic rings or a plurality of condensed ring.Such cycloalkyl comprises, for example, single ring architecture such as cyclopropyl, cyclobutyl, cyclopenta, ring octyl group etc., or multiring structure such as adamantyl and dicyclo [2.2.1] heptane, or with aryl-fused cyclic alkyl, for example indane (indan) etc.
Term " cycloalkyl of replacement " refers to has 1～5 substituent group; preferred 1～3 substituent cycloalkyl, this substituent group is selected from the group of being made up of following group: alkyl; thiazolinyl; alkynyl; alkoxyl; cycloalkyl; cycloalkenyl group; acyl group; acylamino-; acyloxy; amino; aminocarbonyl; alkoxycarbonyl amino; azido; cyano group; halogen; hydroxyl; ketone group; thiocarbonyl; carboxyl; carboxyalkyl; arylthio; the heteroaryl sulfenyl; the heterocyclic radical sulfenyl; mercaptan; alkylthio group; aryl; aryloxy group; heteroaryl; amino-sulfonyl; amino carbonyl amino; heteroaryl oxygen base; heterocyclic radical; the heterocyclyloxy base; hydroxylamino; alkoxy amino; nitro;-SO-alkyl;-SO-aryl;-SO-heteroaryl;-SO 2-alkyl ,-SO 2-aryl and-SO 2-heteroaryl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1～3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nThe substituent group of R (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces.
Term " halogen " or " halo " refer to fluoro, bromo, chloro and iodo.
Term " acyl group " expression group-C (O) R, wherein R is the aryl of the heterocyclic radical of the cycloalkyl of the alkyl of hydrogen, optional replacement, optional replacement, optional replacement, optional replacement and the heteroaryl of optional replacement.
Term " heteroaryl " refers at least one ring and comprises 1～15 carbon atom and 1～4 heteroatomic aromatic group (that is, undersaturated) that is selected from oxygen, nitrogen and sulfur.
Unless qualification is arranged in addition for the heteroaryl substituent definition; such heterocyclic radical can be alternatively by 1～5 substituent group; preferred 1～3 substituent group replaces, and the substituent group here is selected from the group of being made up of following group: alkyl; thiazolinyl; alkynyl; alkoxyl; cycloalkyl; cycloalkenyl group; acyl group; acylamino-; acyloxy; amino; aminocarbonyl; alkoxycarbonyl amino; azido; cyano group; halogen; hydroxyl; ketone group; thiocarbonyl; carboxyl; carboxyalkyl; arylthio; the heteroaryl sulfenyl; the heterocyclic radical sulfenyl; mercaptan; alkylthio group; aryl; aryloxy group; heteroaryl; amino-sulfonyl; amino carbonyl amino; heteroaryl oxygen base; heterocyclic radical; the heterocyclyloxy base; hydroxylamino; alkoxy amino; nitro;-SO-alkyl;-SO-aryl;-SO-heteroaryl;-SO 2-alkyl ,-SO 2-aryl and-SO 2-heteroaryl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1-3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nThe substituent group of R (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces.Such heteroaryl can have monocycle (as, pyridine radicals or furyl) or a plurality of condensed ring (as, indyl (indolizinyl), benzothiazole or benzothienyl).The example of azacyclo-and heteroaryl comprises, but be not limited to the pyrroles, imidazoles, pyrazoles, pyridine, pyrazine, pyrimidine, pyridazine, benzazole, iso-indoles, indole, indazole, purine, quinolizine, isoquinolin, quinoline, phthalazines, naphthyl pyridine (naphthylpyridine), quinoxaline, quinazoline, cinnolines (cinnoline), pteridine (pteridine), carbazole, carboline, phenanthridines, acridine, phenanthroline (phenanthroline), isothiazole, azophenlyene isoxazole phenoxazine, phenothiazine, imidazolidine, imidazoline or the like, and the heterocyclyl compounds that contains N-alkoxyl-nitrogen.
Term " heteroaryl oxygen base " refers to group heteroaryl-O-.
Term " heterocyclic radical " refers to the saturated or part unsaturated group of the unit price with monocycle or a plurality of condensed ring, and it has 1～40 carbon atom and 1～10 hetero atom on ring, preferred 1～4 hetero atom, and this hetero atom is selected from nitrogen, sulfur, phosphorus and/or oxygen.
Formula I chemical compound comprises the definition of " heterocyclic radical that R and YR ' and the nitrogen-atoms that links to each other with them are represented optional replacement ".Such definition is included in the heterocycle that only has nitrogen in the ring, for example pyrrolidine and piperidines, and also comprise and contain heteroatomic heterocycle more than in the ring, for example, piperazine, morpholine etc.
Unless the definition for heterocyclic substituent has qualification in addition; such heterocyclic radical is alternatively by 1～5 substituent group; preferred 1～3 substituent group replaces, and this substituent group is selected from the group of being made up of following group: alkyl; thiazolinyl; alkynyl; alkoxyl; cycloalkyl; cycloalkenyl group; acyl group; acylamino-; acyloxy; amino; aminocarbonyl; alkoxycarbonyl amino; azido; cyano group; halogen; hydroxyl; ketone group; thiocarbonyl; carboxyl; carboxyalkyl; arylthio; the heteroaryl sulfenyl; the heterocyclic radical sulfenyl; mercaptan; alkylthio group; aryl; aryloxy group; heteroaryl; amino-sulfonyl; amino carbonyl amino; heteroaryl oxygen base; heterocyclic radical; the heterocyclyloxy base; hydroxylamino; alkoxy amino; nitro;-SO-alkyl;-SO-aryl;-SO-heteroaryl;-SO 2-alkyl ,-SO 2-aryl and-SO 2-heteroaryl.Unless definition has qualification in addition, all substituent groups can further be selected from alkyl, carboxyl, carboxyalkyl, aminocarbonyl, hydroxyl, alkoxyl, halogen, CF by 1-3 alternatively 3, amino, the amino that replaces, cyano group and-S (O) nThe substituent group of R (wherein R is that alkyl, aryl or heteroaryl and n are 0,1 or 2) replaces.Heterocyclic radical can have monocycle or a plurality of condensed ring.Typical heterocycle comprises tetrahydrofuran base, morpholino, piperidyl etc.
Term " mercaptan " refers to group-SH.
Term " alkylthio group of replacement " refers to the alkyl that group-S-replaces.
Term " heteroaryl sulfenyl " refers to group-S-heteroaryl, and wherein heteroaryl comprises also the heteroaryl of optional replacement as defined above as defined above.
Term " sulfoxide " refers to gene-S (O) R, and wherein R is alkyl, aryl, or heteroaryl." sulfoxide of replacement " refers to group-S (O) R, and wherein R is the alkyl that replaces, the aryl of replacement, or the heteroaryl that replaces, as definition in this article.
Term " sulfone " refers to group-S (O) 2R, wherein R is alkyl, aryl, or heteroaryl." sulfone of replacement " refers to group-S (O) 2R, wherein R is the alkyl that replaces, the aryl of replacement, or the heteroaryl that replaces, as definition in this article.
Term " ketone group " refer to group-C (O)-.Term " thiocarbonyl " refer to group-C (S)-.Term " carboxyl " refers to group-C (O)-OH.
" optional " or " alternatively " meaning is that incident or the situation of describing subsequently can take place or can not take place, and this description comprises situation that wherein said incident or situation take place and the situation that does not take place.
Term " formula I chemical compound " is used for containing as disclosed The compounds of this invention, with pharmaceutically acceptable salt, pharmaceutically acceptable solvate, for example, but be not limited to, pharmaceutically acceptable hydrate, pharmaceutically acceptable ester, and the prodrug of such chemical compound (prodrug).In addition, The compounds of this invention can have one or more asymmetric centers, and can be used as racemic mixture or as independent enantiomer or diastereomer production.The stereoisomer number that exists in any formula I chemical compound that provides all depends on the asymmetric center number (when n is the number of asymmetric center, having 2n stereoisomer) of existence.Independent stereoisomer can pass through to split in synthetic certain suitable stage the raceme or the non-racemic mixture of (resolve) intermediate, or by obtaining with traditional approach split-type I chemical compound.The raceme and the non-racemic mixture of independent stereoisomer (comprising independent enantiomer and diastereomer) and stereoisomer all comprise within the scope of the invention, it all will be described by framework of the present invention, indicate unless have specifically in addition.
" isomer " is the different chemical compounds with same molecular formula.
" stereoisomer " be only atom in the different isomer of spatial arrangements mode.
" enantiomer " is a pair of stereoisomer that can not eclipsed mirror image each other.1: 1 mixture of a pair of stereoisomer is " raceme " mixture.Term " (±) " is used for labelling racemic mixture in due course.
" diastereomer " is the stereoisomer with at least two asymmetric atoms, but their mirror images each other not.
The absolute stereo chemical formula is determined according to Cahn-Ingold-Prelog R-S system.When this chemical compound is pure enantiomer, refer to that the spatial chemistry at each chiral carbon place can be expressed as R or S.The fractionation chemical compound of absolute configuration the unknown can make the direction (dextrorotation or left-handed) of the plane rotation of polarized light be labeled as (+) or (-) under the sodium D-line wavelength according to them.
Term " treatment effective dose " refers to the amount of formula I chemical compound, and when needing the mammal of such treatment, it is enough to reach the purpose of effective treatment, as following definition.The treatment effective dose will be according to variations such as the seriousness of subject object and the disease patient's condition, object body weight and age, the disease patient's condition, administering modes, and this is easy to be determined by those skilled in the art.
Term " coronary artery disease " is meant the chronic disease that wherein has coronary artery " hardening " (atherosclerosis).
Term " atherosclerosis " refers to arteriosclerotic a kind of form, and the light yellow speckle that wherein contains cholesterol, lipid material and lipophage forms in the inner membrance of large artery trunks and medium-sized artery and interior media (inner media).
Term " treatment " or " processing " are meant any treatment to mammalian diseases, comprising:
I) prevent disease promptly, does not develop the clinical symptoms of disease;
Ii) suppress disease, that is, and the development of containment clinical symptoms; And/or
Iii) alleviate disease, that is, clinical symptoms is disappeared.
Under many circumstances, owing to have amino and/or carboxyl or group similar with it, The compounds of this invention can form acid/basic salt.Term " pharmaceutically acceptable salt " but refer to the biological efficacy of hold mode I chemical compound and the salt of character, and it is not biology or does not expect in other respects.Pharmaceutically acceptable base addition salts can be by inorganic and organic base preparation.Come from the salt of inorganic base, comprise, only for giving an example sodium, potassium, lithium, ammonium, calcium and magnesium salt.The salt that comes from organic base comprises, but be not limited to primary amine, the salt of secondary amine and tertiary amine, alkylamine for example, dialkylamine, trialkylamine, the alkylamine that replaces, two (alkyl of replacement) amine, three (alkyl of replacement) amine, alkenyl amine, dialkylene amine, trialkenyl amine, the alkenyl amine that replaces, two (thiazolinyl of replacement) amine, three (thiazolinyl of replacement) amine, Cycloalkyl amine, two (cycloalkyl) amine, three (cycloalkyl) amine, the Cycloalkyl amine that replaces, dibasic Cycloalkyl amine, trisubstituted Cycloalkyl amine, cycloalkenyl group amine, two (cycloalkenyl group) amine, three (cycloalkenyl group) amine, the cycloalkenyl group amine that replaces, dibasic cycloalkenyl group amine, trisubstituted cycloalkenyl group amine, arylamine, diaryl amine, triarylamine, heteroaryl amine, two heteroaryl amine, three heteroaryl amine, heterocyclic amine, two heterocyclic amines, three heterocyclic amines, blended diamidogen and triamine, wherein having two differences at least and be selected from the substituent group on the amine by alkyl, the alkyl that replaces, thiazolinyl, the thiazolinyl that replaces, cycloalkyl, the cycloalkyl that replaces, cycloalkenyl group, the cycloalkenyl group that replaces, aryl, heteroaryl, the group that heterocyclic radical etc. are formed.Comprise that also two or three substituent groups wherein form the amine of heterocyclic radical or heteroaryl with amido nitrogen.
The instantiation of suitable amine comprises, only for giving an example 2-aminopropane., trimethylamine, diethylamine, three (isopropyl) amine, three (n-pro-pyl) amine, ethanolamine, 2-dimethylaminoethanol, trometamol (tromethamine), lysine, arginine, histidine, caffeine, procaine, Hai Baming (hydrabamine), choline (choline), betanin (betaine), ethylenediamine, glucamine, N-alkylated glucamine, theobromine (theobromine), purine, piperazine, piperidines, morpholine, N-ethylpiperidine etc.
Pharmaceutically-acceptable acid addition can be by mineral acid and organic acid preparation.The salt that comes from mineral acid comprises, hydrochloric acid, hydrobromic acid, sulphuric acid, nitric acid, phosphoric acid etc.Come from the salt that organic acid salt comprises acetic acid, propanoic acid, hydroxyacetic acid, acetone acid, oxalic acid, malic acid, malonic acid, succinic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethyl sulfonic acid, p-methyl benzenesulfonic acid, salicylic acid etc.
As used in this article, " pharmaceutically acceptable carrier (carrier) " comprise any and all solvents, disperse medium, coating (coating), antibacterium and antifungal, etc. blend and absorb delayer etc.It is well known in the art being used for the such medium of pharmaceutically active substances and the use of medicament.Except any traditional sucrose or medicament are incompatible with active component, their application in therapeutic combination have been considered.The auxiliary activity composition also can be incorporated in the described compositions.
Nomenclature
The naming ﹠ numbering of The compounds of this invention illustrates with the representative compounds of formula I, and wherein R is a hydrogen, R 1Be 2-hydroxyl cycloalkyl, R 2Be hydrogen, R 3Be the 2-fluorophenyl, R 4And R 5All be hydrogen, and X and Y are covalent bonds:
Figure BPA00001233335000171
This chemical compound is named as:
2-{6-[((1R, 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(2-fluorophenyl sulfenyl) methyl] oxolane (oxolane)-3, the 4-glycol.
The synthetic reaction parameter
Term " solvent ", " inert organic solvents " or " atent solvent " are meant that inert solvent [comprises under the reaction condition that is described in conjunction with it, for example, benzene, toluene, acetonitrile, oxolane (" THF "), dimethyl formamide (" DMF "), chloroform, METHYLENE CHLORIDE (or dichloromethane), diethyl ether, methanol, pyridine etc.].Unless regulation is in contrast arranged, the solvent that uses in the present invention's reaction all is an inert organic solvents.
Term " q.s. " is meant to add is enough to realize described function, and for example, regulator solution is to the amount of intended volume (that is, 100%).
Synthesizing of formula I chemical compound
Formula I chemical compound can be from 2, and the 6-dichloropurine begins preparation, as reaction GraphicShown in the I.
Reaction scheme I
Figure BPA00001233335000181
Figure BPA00001233335000191
Formula I
The preparation of step 1-formula (2)
Formula (1) starting compound as before at United States Patent (USP) 5,789, the method preparation of describing in 416, the full content of this patent is incorporated in this by reference.
Formula (2) chemical compound is normally at atent solvent, for example in the dimethyl formamide, acidic catalyst at catalytic amount, under the existence as p-methyl benzenesulfonic acid, at about 40～90 ℃, typically under about 70 ℃, by with 2, the 2-dimethoxy propane reacted about 24～72 hours, typically about 48 hours, prepared routinely from formula (1) chemical compound.When this reaction was finished substantially, formula (2) product separated by traditional approach, and for example removal of solvent under reduced pressure is also by flash chromatography purification residue.
The preparation of step 2-formula (3)
Formula (2) chemical compound transforms an accepted way of doing sth (3) chemical compound then.In the presence of triphenylphosphine and azoethane dicarboxylic ester, at atent solvent, as, ether or oxolane Chinese style (2) chemical compound and formula R 3The mercaptan compound reaction of SH, wherein R 3As defined above.This reaction typically under refluxing about 24-100 hour, typically about 72 hours.When this reaction was finished substantially, the product of formula (3) can separate by traditional approach, and for example removal of solvent under reduced pressure is also by flash chromatography purification residue.
The preparation of step 3-formula (4)
In the presence of alkali such as triethylamine, react then, by with formula RR 1YNH 2Amine, the 2-cl part is substituted in formula (3) chemical compound, wherein Y is covalent bond or alkylidene.This is reflected in inertia proton solvent such as the ethanol, and reaction is about 14-48 hour under the temperature that refluxes approximately, typically about 24 hours.When this reaction was finished substantially, formula (4) product can separate by traditional approach, for example removal of solvent under reduced pressure and then residue of chromatography on silica gel.
The preparation of step 4-formula I
Then by with acid organic acid for example, as acetic acid treatment, to formula (4) chemical compound deprotection.This is reflected in the mixture of acid and water, under about 50～100 ℃, typically carries out about 10～48 hours typically about 16 hours under about 80～90 ℃.When this reaction was finished substantially, formula I product can separate by traditional approach, for example removal of solvent under reduced pressure and then residue of chromatography on silica gel.
It should be noted that the order that step 2 and 3 can be opposite carries out.
The replaceable of formula I chemical compound synthesizes
Replacedly, the chemical compound of formula I can be prepared as shown in reaction scheme II.
Reaction scheme II
Figure BPA00001233335000221
The preparation of step 1-formula (5)
Resin/the chemical compound of formula (5) is through type (a 1) chemical compound in the presence of the acidic catalyst of catalytic amount such as 10-camphorsulfonic acid (camphorsulfonic acid), in atent solvent such as dimethyl acetylamide, under about room temperature,, be prepared in for example about 4 days with dimethylacetal (dimethylacetal) resin reaction about 1-7 days.When this reaction was finished substantially, the resin/chemical compound of formula (5) can for example filter by traditional approach and separate.
The preparation of step 2-formula (6)
Then in the presence of alkali such as diisopropylethylamine by with formula RR 1YNH 2Amine reaction, the 2-cl part in the resin/chemical compound of formula (5) is substituted, wherein Y is covalent bond or alkylidene.This is reflected at the inertia proton solvent, as 1, in the 4-dioxane, carries out typically about 48 hours about 14～96 hours under about 80 ℃ temperature.When this reaction was finished substantially, the resin/product of formula (6) can separate by traditional approach.
The preparation of step 3-formula (7)
The product of formula (6) transforms the resin/chemical compound of an accepted way of doing sth (7) then.Resin/the chemical compound of formula (6) originally with the chemical compound that can form leaving group, mesyl chloride for example is at alkali, under the existence as diisopropylethylamine, in about 0 ℃ of reaction down.Methylated product then with formula R 3The mercaptan compound of XSH reacts in atent solvent such as acetonitrile solution, wherein R 3With X as defined above.This reaction was typically carried out about 24～100 hours under refluxing,, for example about 70 hours.When this reaction was finished substantially, formula (7) product can for example filter by traditional approach and separate.
The preparation of step 4-formula I
By using acid as organic acid, for example 2% trifluoroacetic acid/5% ethanol/methylene is handled, and makes the resin/chemical compound deprotection of formula (7) then.This is reflected at and carries out about 30 minutes～10 hours under about room temperature, for example about 2 hours.When this reaction was finished substantially, the product of formula I can separate by traditional approach, for example used atent solvent such as dichloromethane extraction, and removed solvent in the extract by reduction vaporization.
Initiation material
R wherein 2Formula (1) chemical compound that is not hydrogen can be by method preparation well known in the art.For example, R wherein 2For the preparation of formula (1) chemical compound of trifluoromethyl is prepared as shown in the response diagram formula III.
The response diagram formula III
Figure BPA00001233335000241
R wherein 2For the preparation of formula (4) chemical compound of nitrile is prepared as shown in reaction scheme IV.
Reaction scheme IV
The initiation material of formula (e)
The initiation material of formula (b) by commercially available (Aldrich, Milwaukee).The product of formula (e) transforms an accepted way of doing sth (4) chemical compound, and is as implied above.
R wherein 2For formula (1) chemical compound of acyl group by 2-stannyl (stannyl)-6-chloro-2 ', 3 ', 5 '-tri-tert dimetylsilyl adenosine (people .J.Org.Chem.1997 such as K.Kato, 62,6833-6841) with the acid chloride reaction and obtain.
Formula I chemical compound also can begin preparation from the 6-chloropurine nucleoside, as shown in reaction scheme V, and R wherein 1Be the 2-hydroxy-cyclopentane, R 2With R be hydrogen, and Y is a covalent bond:
Reaction scheme V
Figure BPA00001233335000261
Wherein Ph is a phenyl.
The preparation of step 1-formula (9)
Formula (9) chemical compound is through type (a 8) chemical compound, in the presence of alkali such as triethylamine, in proton solvent such as ethanol, reacts preparation in about 24 hours with 2-(benzyloxy) cyclopenta amine under the temperature that refluxes approximately.When this reaction is finished substantially, the product of formula (9) can separate by traditional approach, and for example removal of solvent under reduced pressure is distributed residue between ethyl acetate and water, remove the solvent in the organic layer, and precipitate the purification residue by for example recrystallization or from ethyl acetate/hexane.
The preparation of step 2-formula (10)
Formula (9) chemical compound transforms an accepted way of doing sth (10) chemical compound then.In the presence of alkali such as pyridine, in the suspension of the formula in atent solvent such as acetonitrile (9) chemical compound, add thionyl chloride.This reaction was typically carried out under about 0 ℃ about 4 hours, allowed it be raised to ambient temperature overnight then.When this reaction is finished substantially, under reduced pressure concentrate the gained suspension, thus acquisition formula (10) chemical compound, this chemical compound is directly used in next step and need not purification.
The preparation of step 3-formula (11)
Formula (11) chemical compound is undertaken by (10) are dissolved in alkali such as ammonium hydroxide and proton solvent such as the methanol mixture from formula (10) compound.This is reflected at and carries out about 30 minutes under about room temperature.When this reaction was finished substantially, the product of formula (11) can separate by traditional approach, and for example removal of solvent under reduced pressure is distributed residue, and under reduced pressure removed ethyl acetate between ethyl acetate and water.This residue is directly used in next step and need not to be further purified.
The preparation of step 4-formula (12)
By in the presence of catalyst such as palladium dydroxide, handle then, make formula (11) chemical compound deprotection with undersaturated compound cycloalkyl of part such as cyclohexene.Replacedly, ammonium formate can replace undersaturated compound cycloalkyl to use.This is reflected in proton solvent such as the ethanol, typically carries out about 18 hours under refluxing approximately.When this reaction was finished substantially, the product of formula (12) can separate by traditional approach, for example by removal of solvent under reduced pressure, then ground residue.
The preparation of step 5-formula I
Formula (12) chemical compound then with formula R 3The chemical compound of SH reacts as the 2-fluoro thiophenol.This is reflected at polar solvent, typically at N, in the dinethylformamide, in the presence of alkali such as sodium hydroxide, carries out under about 100 ℃ about 3～5 hours.When this reaction was finished substantially, formula I chemical compound can separate by traditional approach, ground residue for example by removal of solvent under reduced pressure, and with diethyl ether.
The preparation of initiation material
2-(benzyloxy)-Aminocyclopentane is as the initiation material in the step 1 of reaction scheme V.This chemical compound as racemic mixture or as independent isomer, or commercially available, or is produced by the well-known method of those skilled in the art.For example, produce (1R, 2R)-a kind of method of 2-(benzyloxy)-Aminocyclopentane is illustrated among the following reaction scheme VI.
Reaction scheme VI
Figure BPA00001233335000281
In first step, for example by in atent solvent, in the presence of the 4-dimethylamino naphthyridine, reacting, use (BOC) by traditional approach 2O (di-tert-butyl dicarbonic acid ester) to the chemical compound of formula (f) ((1R, 2R)-2-Aminocyclopentane-1-alcohol) carries out N-protected.Protected cyclopentanol (g) derivant is reacted in the presence of alkali such as sodium hydride with benzyl bromide a-bromotoluene then, thereby forms (h), then in a conventional manner, for example in dioxane with hydrochloric acid to (h) deprotection.
From (1S 2S)-2-Aminocyclopentane-1-alcohol sets out, provides to have opposite stereochemical chemical compound with formula (i), and from (1RS, 2RS)-2-Aminocyclopentane-1-alcohol sets out, and the raceme analog of formula (i) chemical compound is provided.
Those skilled in the art should be appreciated that and add R in core texture (core structure) 3The SY part can removed R 1Carry out before or after the protecting group on the part, for example the protecting group of the 2-hydroxyl on the 6N cyclopenta shown in the reaction scheme V.A kind of replaceable method has been shown among the reaction scheme VII has utilized different protecting groups and counter-rotating R 3The addition and the R of SY part 1The deprotection of base comes preparation I compound, wherein R 1Be the 2-hydroxy-cyclopentane, R 2With R be hydrogen, Y is a covalent bond.
Reaction scheme VII
Figure BPA00001233335000301
Initial shielded cyclopentyl derivates can derive from (1R, 2R)-2-Aminocyclopentane-1-alcohol, (1S, 2S)-2-Aminocyclopentane-1-alcohol, or (1RS, 2RS)-2-Aminocyclopentane-1-alcohol.Hydroxyl is by method well known in the art, for example by in methanol with NH 4The F reaction, protected as t-butyldimethylsilyl.
Replacedly, synthetic compound of formula i need not to use any protecting group easily, as shown in reaction scheme VIII, and R wherein 1Be the 2-hydroxy-cyclopentane, R 2With R be hydrogen, and Y is a covalent bond.
Reaction scheme VIII
Figure BPA00001233335000311
Need not to use any protecting group, or need not to separate and/or the situation of purify intermediates under, a kind of method of preparation I compound is illustrated among the reaction scheme IV, wherein R 1Be the 2-hydroxy-cyclopentane, R 2With R be hydrogen, and Y is a covalent bond.
Reaction scheme IV
Figure BPA00001233335000321
The preparation of step 1-formula (19)
Formula (8) chemical compound is by transforming an accepted way of doing sth (19) chemical compound with the thionyl chloride reaction.Usually, in the presence of the alkali such as pyridine of about 2～2.5 molar equivalents, formula (8) chemical compound is suspended in atent solvent such as the acetonitrile, and in about 1 hour, slowly adds the thionyl chloride of about 5～5.5 molar equivalents.This reaction was typically carried out under about 0 ℃ about 3 hours, allowed it be raised to ambient temperature overnight then.When this reaction is finished substantially, thus concentrating under reduced pressure gained solution production (19) chemical compound, and this chemical compound is directly used in next step and need not purification.
The preparation of step 3-formula (20)
Formula (20) chemical compound is from formula (19) compound, is dissolved in by the crude product with step 1 in the mixture of proton solvent such as methanol aqueous solution and alkali such as ammonia to carry out.This is reflected at and carried out under about 0 ℃ about 1 hour, then at room temperature carries out about 3 hours.When this reaction was finished substantially, the product of formula (20) can separate by traditional approach, and was directly used in next step and is not further purified.
The preparation of step 4-formula (18)
Formula (18) chemical compound is the crude product (formula (20) chemical compound) by step 3, in the presence of the alkali such as triethylamine of about 3 molar equivalents, in proton solvent such as isopropyl alcohol, under the temperature that refluxes approximately, react preparation in about 24 hours with the 2-hydroxycyclopent amine of about 1～1.1 molar equivalent.When this reaction was finished substantially, the product of formula (18) can separate by traditional approach, for example stirred residue by removal of solvent under reduced pressure and in water.
The preparation of step 5-formula I
The product of step 4 (formula (18) chemical compound) then with the formula R of about 3～5 molar equivalents 3The for example 2-fluoro thiophenol reaction of SH chemical compound.This is reflected at polar solvent, N typically, and in the dinethylformamide, at the alkali of about 5～6 molar equivalents for example sodium hydride, sodium hydroxide, or triethylamine, exist down as triethylamine, under about room temperature, carried out typically about 3 days about 1～5 day.When this reaction was finished substantially, the product of formula I can separate by traditional approach.This product can come other purification as recrystallization in methanol, ethanol, isopropyl alcohol or methanol and the alcoholic acid mixture by from all kinds of solvents again.Replacedly, this product can be by recrystallization from ethyl acetate or by coming purification with ethyl acetate pulp (slurrying).
Effectiveness, test and administration
General utility
Formula I chemical compound is effectively useful in the disease in the mammal of treatment or the disease at the cellular expression of the available raising of treatment ABCA-1 gene and/or the chemical compound that improves the ABCA-1 protein expression.Such disease includes, but are not limited to, arterial disease, particularly coronary artery disease.In some embodiments, the feature of this disease is that the HDL cholesterol is low.In some embodiments, this disease or disease can be one or more in diabetes, insulin resistant, dyslipidemia, coronary artery disease and the inflammation.
Test
By above-cited those patents and list of references, and described in the following example, and by being that conspicuous method is carried out active testing to those skilled in the art.
Pharmaceutical composition
The chemical compound of formula I is usually with the pharmaceutical compositions administration.Therefore the invention provides pharmaceutical composition, it contains as in the formula I chemical compound of active component one or more, or its pharmaceutically acceptable salt or ester, and one or more pharmaceutically acceptable excipient (excipient), carrier (comprising inert solid diluent and filler), diluent (comprising aseptic aqueous solution and various organic solvent), penetration enhancer, solubilizing agent and adjuvant.Formula I chemical compound can be individually dosed or with other treatment agent administering drug combinations.Such compositions can pharmaceutical field in well-known mode prepare (referring to, for example, Remington ' sPharmaceutical Sciences, Mace Publishing Co., Philadelphia, PA 17 ThEd. (1985) and " Modern Pharmaceutics ", Marcel Dekker, Inc.3 RdEd.G.S.Banker﹠amp; C.T.Rhodes, Eds.).
Administration
Formula I chemical compound can be by having similar effectiveness the accepted administering mode of medicament in any, with single dose or multiple dose administration, for example be incorporated in by reference herein those patents and patent application described in, comprise rectum, buccal, nasal cavity and transdermal path, by intra-arterial injection, intravenous administration, intraperitoneal administration, parenteral, intramuscular administration, subcutaneous administration, oral, administration partly, as inhalant, or via dipping or apparatus for coating, support for example is as inserting the cylindrical polymeric of tremulous pulse.
A kind of mode of administration is a parenteral, particularly by injection.The form that is used for drug administration by injection that the novel compositions of the present invention can be integrated, comprise water or oil suspension or emulsion, utilize Oleum sesami, Semen Maydis oil, Oleum Gossypii semen or Oleum Arachidis hypogaeae semen, and elixir, mannitol, glucose, or aseptic aqueous solution, with similar pharmaceutical carrier (vehicle).Saline solution also is generally used for injection.Also can adopt (and suitable mixture), cyclodextrin derivative such as ethanol, glycol, propylene glycol, liquid polyethylene glycol, and vegetable oil.For example, under dispersive situation, use surfactant, can keep suitable flowability by keeping required granular size and passing through by using coating such as lecithin.The prevention of microbial action can be passed through various antibacterial agents and antifungal, for example P-hydroxybenzoic acid lipid (parabens), chlorobutanol, phenol, sorbic acid, thimerosal generations such as (thimerosal).
Sterile injectable liquid can by as required will be in appropriate solvent aequum formula I chemical compound and above-named various other compositions are mixed together and subsequent filtration sterilization preparation.Usually, dispersion liquid can prepare by various active component through sterilization are incorporated in the sterile carrier that contains basic disperse medium and above-named required other compositions.Under the situation of the sterilized powder that is used to prepare aseptic parenteral solution, the typical method of preparation is vacuum drying and Freeze Drying Technique, and this technology can produce active component powder and any other desired constituents from its previous sterilising filtration solution.
Oral administration is another path of formula I compound administration.Can be via administrations such as capsule or enteric coatel tablets.In producing the pharmaceutical composition that comprises at least a formula I chemical compound, active component is diluted by excipient usually and/or is encapsulated in such carrier, to present the form of capsule, wafer (sachet), paper or other containers.When excipient was used as diluent, it can be solid, semisolid or fluent material (as above), and it serves as excipient, carrier or the medium of active component.Thereby, the form of said composition can be tablet, pill, powder, lozenge, wafer, cachet (cachets), elixir, suspension, emulsion, solution, syrup, aerosol (as solid or in liquid medium), for example contain nearly unguentum, soft hard capsule, the aseptic parenteral solution of the reactive compound of 10 weight %, and aseptic packaging powder.
Some examples of suitable excipient comprise lactose, glucose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, Tragacanth, gelatin, calcium silicates, microcrystalline Cellulose, polyvinylpyrrolidone, cellulose, sterilized water, syrup, and methylcellulose.Preparation can comprise in addition: lubricant such as Pulvis Talci, magnesium stearate and mineral oil; Wetting agent; Emulsifying agent and suspending agent; Antiseptic such as essence of Niobe and benzoic acid hydroxypropyl acrylate; Sweetener; And flavoring agent.
By adopting method as known in the art, prepare compositions of the present invention, so that after patient's administration, providing quick, lasting or delaying release of active ingredients.The controlled-release administrating system that is used for oral administration comprises the osmotic pump system and the dissolution system that contain polymer-coated container or drug-polymer matrix formulations.The example of controlled-release administrating system is in U.S. Patent No. 3,845, provides in 770,4,326,525,4,902514 and 5,616,345.The another kind of preparation that uses in the inventive method has adopted transdermal drug delivery systems (" patch ").Such transdermal patch can be used to provide the The compounds of this invention in the continuous or discrete perfusion controlled quatity.The structure and the purposes that are used to pass the transdermal patch of medicine are well-known in the art.Referring to, as U.S. Patent No. 5,023,252,4,992,445 and 5,001,139.Such patch can make up and be used for continuous, pulse, or sends medicament when needed.
Compositions typically is mixed with unit dosage forms.Term " unit dosage forms " refers to physically separated unit, be suitable as and be used for people's object and other mammiferous single doses, each unit contains as calculated the active material with the scheduled volume that produces the desired therapeutic effect, and suitable drug excipient (as, tablet, capsule and injection).Formula I chemical compound is effectively in wide dosage range, and usually with pharmaceutically effective dose administration.Typically, for oral administration, each dosage unit contains 10mg～2g, more typically contain the formula I chemical compound of 10mg～700mg, and for parenteral, every dosage unit contains 10mg～700mg, more typically contains the formula I chemical compound of 50mg～200mg.Yet, be to be understood that, the amount of the actual formula I chemical compound that gives should be determined according to relevant situation by the doctor, comprises the seriousness of the disease that will treat, the administration path of selection, the pragmatize compound that gives and relative activity, each patient's age, body weight and reaction, patient's symptom etc.
In order to prepare solid composite, tablet for example, thus main active mixed the solid preformulation composite that forms the homogeneous mixture that contains The compounds of this invention with drug excipient.When mentioning these compositions formulated when even, the meaning is meant that active component is dispersed in the whole compositions fifty-fifty, so that said composition can be subdivided into the unit dosage forms of equivalence at an easy rate, as tablet, pill and capsule.
Tablet of the present invention or pill can carry out coating or prepare by other modes again, thereby the dosage form that prolongs action time is provided, or protect it to avoid the influence of the acid condition of stomach.For example, tablet or pill can comprise internal dose component and outside dosage component, and the latter covers the former with the form of sealing.These two kinds of components can separate with enteric layer, and wherein enteric layer is used for stoping component disintegrate and make internal composition intactly send into duodenum or delay to discharge under one's belt.Various materials all can be used for such enteric layer or coating, and such material comprises many polymeric acids and polymeric acid and the such material such as the mixture of lac, hexadecanol and cellulose acetate.
The compositions that is used to suck or be blown into is included in pharmaceutically acceptable water or organic solvent, or the solution in its mixture, suspension, and powder.Liquid and solid composite can contain suitable pharmaceutically acceptable excipient, and be described above.In some embodiments, but compositions through port or nasal respiration path give, to produce part or systemic effect.Compositions in pharmaceutically acceptable solvent can atomize by using noble gas.The solution of atomizing can directly suck from atomising device, perhaps atomising device can with face tent, or the batch (-type) positive pressure respirator is connected.Solution, suspension, or powder composition can be from the device of delivery formulation in a suitable manner, and typically oral or intranasal carries out administration.
Following example is used for example explanation typical embodiment more of the present invention.It will be appreciated by those skilled in the art that disclosed technology in the example of abideing by the representative technology that the present inventor finds, in practice of the present invention, be able to fine playing a role, thereby can think that it is configured for the example of its practice.Yet those skilled in the art should be appreciated that according to present disclosure, under situation without departing from the spirit and scope of the present invention, can also still can obtain same or analogous result to making many changes in the disclosed specific embodiment.
Example 1
The preparation of formula (2) chemical compound
A. the preparation of formula (2) chemical compound, wherein R 3 Be hydrogen
Figure BPA00001233335000381
To 2-(6-chloropurine-9-yl)-5-hydroxymethyl tetrahydrofuran-3, (4.9g is 17.1mmol) with 2 for 4-glycol (formula (1) chemical compound), 2-dimethoxy propane (10.5mL, 84.7mmol) solution in dimethyl formamide (100mL), add p-methyl benzenesulfonic acid (325mg, 1.71mmol).After 70 ℃ are stirred 24 hours down, the vacuum concentration reactant liquor also passes through flash column chromatography (70%EtOAc/ hexane) purification residue, thereby the formula of obtaining (2) chemical compound, 6-(6-chloropurine-9-yl)-2,2-dimethyl-tetrahydrofuran [3,4-d] [1,3] dioxo-4-yl] methanol, be pale solid.(3.8g，68％) 1HNMR(CDCl 3)δ1.4(s，3H)，1.65(s，3H)，3.8-4.0(dd，2H)，4.6(s，1H)，5.1-5.3(m，2H)，6.0(d，1H)，8.25(s，1H)，8.8(s，1H)。
B. the preparation of formula (2) chemical compound changes R2
Similarly, according to the method step of top 1A, but replace 2-(6-chloropurine-9-yl)-5-hydroxymethyl tetrahydrofuran-3 with other formula (1) chemical compound, the 4-glycol prepares other formula (2) chemical compound.
Example 2
The preparation of formula (3) chemical compound
A. the preparation of formula (3) chemical compound, wherein R 2 Be hydrogen, R 3 Be the 2-fluorophenyl, and X It is covalent bond
Figure BPA00001233335000391
To formula (2) chemical compound, 6-(6-chloropurine-9 base)-2,2-dimethyl-tetrahydrofuran [3,4-d] [1,3] dioxo-4-yl] methanol (0.48g, 1.47mmol) solution in the 20mL oxolane, add triphenylphosphine (0.77g, 2.94mmol) and the azoethane dicarboxylic ester (0.47mL 2.94mmol), and stirred this mixture 5 minutes.(0.31mL 2.94mmol), refluxes and stirs this mixture down to add the 2-fluoro thiophenol then.After refluxing 72 hours, the vacuum concentration reactant also passes through flash column chromatography (20%EtOAc/ hexane) purification residue, thus the formula of obtaining (3) chemical compound, 1-{[(2S, 1R, 4R, 5R)-4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 2-fluorobenzene, be limpid viscous oil (3) (0.25g,～40%)
1H?NMR(CDCl 3)δ1.4(s，3H)，1.6(s，3H)，3.2(m，2H)，4.6(t，1H)，5.1(m，1H)，5.5(m，1H)，6.0(d，1H)，7.0(m，2H)，7.2(m，1H)，7.4(m，1H)，8.25(s，1H)，8.75(s，1H)。
B. the preparation of formula (3) chemical compound changes R 2 And R 3
Similarly,, but use other formula (2) chemical compound to replace 6-(6-chloropurine-9 base)-2,2-dimethyl-tetrahydrofuran [3,4-d] [1,3] dioxo-4-yl alternatively according to the method step of top 2A] methanol, and use other formula R alternatively 3The XH chemical compound replaces the 2-fluoro thiophenol, has prepared following formula (3) chemical compound.
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9-yl)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto } benzene;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-2, the 6-dichloro-benzenes;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9-yl)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-2,4 difluorobenzene;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 4-fluorobenzene;
2-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 4-methyl isophthalic acid, the 3-thiazole;
2-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-1, the 3-benzoxazole;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-2-toluene;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto)-the 2-chlorobenzene;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 4-chlorobenzene;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 2-fluorobenzene;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 3-fluorobenzene;
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 2-thiophene; With
1-{[(2S, 1R, 4R, 5R)-and 4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methoxyl group }-the 2-fluorobenzene.
B. the preparation of formula (3) chemical compound changes R 2 And R 3
Similarly,, but use other formula (2) chemical compound to replace 6-(6-chloropurine-9 base)-2,2-dimethyl-tetrahydrofuran [3,4-d] [1,3] dioxo-4-yl alternatively according to the method step of top 2A] methanol, and use other formula R alternatively 3The XH chemical compound replaces the 2-fluoro thiophenol, has prepared other formula (3) chemical compound.
Example 3
The preparation of formula (4) chemical compound
A. the preparation of formula (4) chemical compound, wherein R is a hydrogen, R 1 Be cyclopenta, R 2 Be hydrogen, R 3 Be the 2-fluorophenyl, and X and Y are covalent bonds
Figure BPA00001233335000411
Chemical compound to formula 3,1-{[(2S, 1R, 4R, 5R)-4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-(0.125g is 2.86mmol) in the solution in 10mL ethanol and 1mL triethylamine for the 2-fluorobenzene, add excessive Aminocyclopentane, this mixture was refluxed under nitrogen 24 hours.Decompression removes down and desolvates, and by with 1: 1 EtOAc: hexane is by preparation property TLC purification residue, thereby the formula of obtaining (4) chemical compound (9-{ (4S, 1R, 2R, 5R)-and 4-[(2-fluorophenyl sulfenyl) methyl]-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl } purine-6-yl) Aminocyclopentane, be xanchromatic grease (80mg, 56%).
1H?NMR(CDCl 3)δ1.4(s，3H)，1.6(s，3H)，1.6-2.4(m，6H)，3.15-3.25(m，2H)，4.1(bs，1H)，4.4(t，1H)，5.1(m，1H)，5.5(m，1H)，6.0(d，1H)，6.2(bs，1H)，7.0(m，2H)，7.2(m，1H)，7.4(m，1H)，7.8(s，1H)，8.25(s，1H)。
B. the preparation of formula (4) chemical compound changes R 1 , R 2 , R 3 , and Y
Similarly, according to the method step of top 3A, but use other formula (3) chemical compound to replace 1-{[(2S, 1R alternatively, 4R, 5R)-4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 2-fluorobenzene, and use other formula R alternatively 1YNH 2Chemical compound replaces Aminocyclopentane, has also prepared following formula (4) chemical compound, and wherein R is a methyl, R 1Be 2-(3, the 4-Dimethoxyphenyl) ethyl, R 2Be hydrogen, and X and Y are covalent bonds:
R 3Be 2, the 6-Dichlorobenzene base;
R 3It is 4-methylthiazol-2-base;
R 3Be 1,3-benzoxazole-2-base;
R 3It is the 2-aminomethyl phenyl;
R 3It is the 2-chlorphenyl; With
R 3It is the 4-chlorphenyl.
C. the preparation of formula (4) chemical compound changes R 1 , R 2 , R 3 And Y
Similarly, according to the method step of top 3A, but use other formula (3) chemical compound to replace 1-{[(2S, 1R alternatively, 4R, 5R)-4-(6-chloropurine-9 base)-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl] methyl mercapto }-the 2-fluorobenzene, and use other formula R alternatively 1YNH 2Chemical compound replaces Aminocyclopentane, has also prepared other formula (4) chemical compound.
Example 4
The preparation of formula I chemical compound
A. the preparation of formula I chemical compound, wherein R is a hydrogen, R 1 Be cyclopenta, R 2 Be hydrogen, R 3 Be The 2-fluorophenyl, and X and Y are covalent bonds
Formula I
Chemical compound (9-{ (4S with formula (4), 1R, 2R, 5R)-and 4-[(2-fluorobenzene sulfenyl) methyl]-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl } purine-6-yl) Aminocyclopentane (50mg) is dissolved in the mixture of acetic acid (8mL) and water (2mL), and 90 ℃ of heating 16 hours down.Decompression removes down and desolvates, and with preparation property TLC[methanol-dichloromethane (1: 9)] the purification residue, thereby make formula I chemical compound (4S, 5S, 3R)-2-[6-(Aminocyclopentane base) purine-9-yl]-5-[(2-fluorobenzene sulfenyl) methyl] oxolane-3, the 4-glycol,
1H?NMR(CDCl 3)δ1.6-2.4(m，6H)，3.15-3.25(m，2H)，4.1(bs，1H)，4.4-4.65(m，4H)，6.0(d，1H)，6.8(bs，1H)，7.05(m，2H)，7.2(m，1H)，7.4(m，1H)，7.8(s，1H)，8.25(s，1H)。
B. the preparation of formula I chemical compound changes R 1
Similarly, method step according to top 4A, but formula (4) chemical compound with other replaces (9-{ (4S, 1R, 2R, 5R)-and 4-[(2-fluorobenzene sulfenyl) methyl]-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl } purine-6-yl) Aminocyclopentane, produced following formula I chemical compound, wherein R, R 2, R 4And R 5Be hydrogen, R 3Be the 2-fluorophenyl, X and Y are covalent bonds, and R 1Be:
Cyclopenta;
(R, R)-2-hydroxycyclopent base;
(R, S)-2-hydroxycyclopent base;
Dicyclo [2.2.1] heptan-2-base;
7,7-dimethyl dicyclo [2.2.1] heptan-2-base;
Dicyclo [2.2.1] heptan-2-base-3-carboxylic acid, ethyl ester;
Dicyclo [2.2.1] heptan-2-base-3-carboxylic acid;
Dicyclo [2.2.1] heptan-2-base-3-methanol;
Cyclopenta-2-carboxylic acid, ethyl ester;
Cyclopenta-2-carboxylic acid;
(R) 2-hydroxy-cyclohexyl;
(S) 2-hydroxy-cyclohexyl;
(R)-the 1-phenylethyl;
(S)-the 1-phenylethyl;
(4-fluorophenyl) methyl;
4-Trifluoromethoxyphen-l methyl;
2,6-difluorophenyl methyl;
(3-methoxyphenyl) methyl;
(4-methoxyphenyl) methyl;
2-benzyloxy cyclopenta;
(4-aminomethyl phenyl) ethyl;
Furan-2-base;
Phenycyclopropyl;
Ethyl 3--propanoate;
Cyclohexyl;
1-(4-methoxyphenyl) ethyl;
3-trifluoromethylbenzene ylmethyl;
3, the 5-dichlorophenylmethyl;
(3-fluorophenyl) methyl;
(2-trifluoromethyl) methyl;
(4-chlorphenyl) methyl;
(2-fluorophenyl) methyl;
2-chloro-4-fluorophenyl methyl;
2-fluoro-4-trifluoromethylbenzene ylmethyl;
2,4-Dichlorobenzene base ethyl;
(R)-the 2-phenyl propyl;
(S)-the 2-phenyl propyl;
2-(3-fluorophenyl) ethyl;
2-(2-chlorphenyl) ethyl;
6,6-dimethyl dicyclo [3.3.1] heptan-3-base;
4-(tert-butyl group) cyclohexyl;
The 2-Chlorophenylmethyl;
1-(4-tolyl) ethyl;
(3-tolyl) methyl;
(4-tolyl) methyl;
2-trifluoromethyl-5-fluorophenyl methyl;
2-chloro-3-trifluoromethylbenzene ylmethyl;
2,6,6-trimethyl dicyclo [3.3.1] heptan-3-base;
The 1-naphthyl methyl;
Dicyclo [3.1.1] heptan-3-base;
2-isopropyl-4-methylcyclohexyl;
2-carboxamido cyclohexyl;
(R)-2-carboxyl cyclohexyl;
(S)-2-carboxyl cyclohexyl;
2-methylol cyclohexyl;
2-carboxylic acid cyclohexyl ethyl ester;
2-carboxyl-4-benzyl ring hexyl;
2-carboxyl dicyclo [2.2.1] heptan-5-alkene-3-base; With
2-carboxylic acid dicyclo [2.2.1] heptan-3-base ethyl ester.
Similarly, prepared following formula I chemical compound, wherein R, R 2, R 4And R 5Be hydrogen, and X and Y are covalent bonds:
R 3Be 4-fluorophenyl and R 1It is cyclopenta;
R 3Be 2-aminomethyl phenyl and R 1It is cyclopenta; With
R 3Be 2,4 difluorobenzene base and R 1It is cyclopenta.
C. the preparation of formula I chemical compound changes R 1 , R 2 , R 3 , R 4 , R 5 , X and Y
Similarly, method according to top 4A, or the combination of use-case 5-8 is synthetic, but uses other formula (4) chemical compound to replace (9-{ (4S, 1R alternatively, 2R, 5R)-and 4-[(2-fluorobenzene sulfenyl) methyl]-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl } purine-6-yl) Aminocyclopentane, produced following formula I chemical compound.
Figure BPA00001233335000471
Figure BPA00001233335000481
Figure BPA00001233335000491
Figure BPA00001233335000501
Figure BPA00001233335000511
Figure BPA00001233335000521
Figure BPA00001233335000531
Figure BPA00001233335000541
Figure BPA00001233335000551
Figure BPA00001233335000561
Figure BPA00001233335000571
Figure BPA00001233335000581
Figure BPA00001233335000591
Figure BPA00001233335000601
Figure BPA00001233335000611
Figure BPA00001233335000621
Figure BPA00001233335000631
R 3 R、R 1Combination with the N atom that links to each other with them
2, the 4-Dichlorobenzene base Piperidines-1-base
2, the 4-Dichlorobenzene base 2-ethyl piperidine-1-base
2, the 4-Dichlorobenzene base 4-(piperidines-1-yl) piperidines-1-base
2, the 4-Dichlorobenzene base 1,2,3,4-tetrahydroisoquinoline-2-base
2, the 4-Dichlorobenzene base Morpholine-4-base
The 2,4 difluorobenzene base 4-methyl piperazine-1-base
The 2,4 difluorobenzene base Pyrrolidine-1-base
The 2,4 difluorobenzene base 4-benzyl diethylenediamine-1-base
The 2,4 difluorobenzene base Piperidines-1-base
The 2,4 difluorobenzene base 4-(piperidines-1-yl) piperidines-1-base
The 2,4 difluorobenzene base 1,2,3,4-tetrahydroisoquinoline-2-base
The 2,4 difluorobenzene base Morpholine-4-base
The 2,4 difluorobenzene base 4-methyl piperazine-1-base
The 4-fluorophenyl 4-benzyl diethylenediamine-1-base
The 4-fluorophenyl Piperidines-1-base
The 4-fluorophenyl 2-ethyl piperidine-1-base
The 4-fluorophenyl 4-benzyl piepridine-1-base
The 4-fluorophenyl 4-(piperidines-1-yl) piperidines-1-base
The 4-fluorophenyl 1,2,3,4-tetrahydroisoquinoline-2-base
The 4-fluorophenyl Morpholine-4-base
The 4-fluorophenyl 4-phenylpiperazine-1-base
The 4-methyl isophthalic acid, the 3-thiazol-2-yl Pyrrolidine-1-base
The 4-methyl isophthalic acid, 3 thiazol-2-yls 4-benzyl diethylenediamine-1-base
The 4-methyl isophthalic acid, the 3-thiazol-2-yl Piperidines-1-base
The 4-methyl isophthalic acid, the 3-thiazol-2-yl 4-benzyl piepridine-1-base
The 4-methyl isophthalic acid, 3 thiazol-2-yls 4-(piperidines-1-yl) piperidines-1-base
The 4-methyl isophthalic acid, the 3-thiazol-2-yl 1,2,3,4-tetrahydroisoquinoline-2-base
The 4-methyl isophthalic acid, the 3-thiazol-2-yl Morpholine-4-base
The 4-methyl isophthalic acid, the 3-thiazol-2-yl 4-methyl piperazine-1-base
The 4-methyl isophthalic acid, the 3-thiazol-2-yl 4-phenylpiperazine-1-base
1,3-benzoxazole-2-base Pyrrolidine-1-base
1,3-benzoxazole-2-base 2-ethyl piperidine-1-base
1,3-benzoxazole-2-base 4-benzyl piepridine-1-base
1,3-benzoxazole-2-base Morpholine-4-base
1,3-benzoxazole-2-base 4-methyl piperazine-1-base
The 2-tolyl Pyrrolidine-1-base
The 2-tolyl Piperidines-1-base
The 2-tolyl 2-ethyl piperidine-1-base
The 2-tolyl 4-benzyl piepridine-1-base
The 2-tolyl 4-(piperidines-1-yl) piperidines-1-base
The 2-tolyl 1,2,3,4-tetrahydrochysene-isoquinolin-2-base
The 2-tolyl Morpholine-4-base
The 2-tolyl 4-(3, the 4-Dichlorobenzene base) piperazine-1-base
The 2-tolyl 4-methyl piperazine-1-base
The 2-tolyl 4-phenylpiperazine-1-base
The 2-tolyl Pyrrolidine-1-base
The 2-chlorphenyl 4-benzyl diethylenediamine-1-base
The 2-chlorphenyl Piperidines-1-base
The 2-chlorphenyl 2-ethyl piperidine-1-base
The 2-chlorphenyl 4-benzyl piepridine-1-base
The 2-chlorphenyl 4-(piperidines-1-yl) piperidines-1-base
The 2-chlorphenyl 1,2,3,4-tetrahydroisoquinoline-2-base
The 2-chlorphenyl Morpholine-4-base
The 2-chlorphenyl 4-(3, the 4-Dichlorobenzene base) piperazine-1-base
The 2-chlorphenyl 4-methyl piperazine-1-base
The 2-chlorphenyl 4-phenylpiperazine-1-base
The 4-chlorphenyl Pyrrolidine-1-base
The 4-chlorphenyl 4-benzyl diethylenediamine-1-base
The 4-chlorphenyl Piperidines-1-base
The 4-chlorphenyl 2-ethyl piperidine-1-base
The 4-chlorphenyl 4-(piperidines-1-yl) piperidines-1-base
The 4-chlorphenyl 1,2,3,4 ,-tetrahydroisoquinoline-2-base
The 4-chlorphenyl Morpholine-4-base
The 4-chlorphenyl 4-phenylpiperazine-1-base
The 2-fluorophenyl Pyrrolidine-1-base
The 2-fluorophenyl 4-benzyl diethylenediamine-1-base
The 2-fluorophenyl Piperidines-1-base
The 2-fluorophenyl 2-ethyl piperidine-1-base
The 2-fluorophenyl Morpholine-4-base
The 2-fluorophenyl 4-phenylpiperazine-1-base
The 2-fluorophenyl Pyrrolidine-1-base
The 2-fluorophenyl 4-benzyl diethylenediamine-1-base
The 3-fluorophenyl Piperidines-1-base
The 3-fluorophenyl 4-benzyl piepridine-1-base
The 3-fluorophenyl 1,2,3,4-tetrahydrochysene-isoquinolin-2-base
The 3-fluorophenyl Morpholine-4-base
The 3-fluorophenyl 4-methyl piperazine-1-base
The 3-fluorophenyl 4-(piperidines-1-yl) piperidines-1-base
Thiophene-2-base 4-phenylpiperazine-1-base
Thiophene-2-base 2-ethyl piperidine-1-base
Phenyl Pyrrolidine-1-base
Phenyl 4-benzyl diethylenediamine-1-base
Phenyl Piperidines-1-base
Phenyl. 2-ethyl piperidine-1-base
Phenyl 4-Phenylpiperidine-1-base
Phenyl 4-(piperidines-1-yl) piperidines-1-base
Phenyl Morpholine-4-base
Phenyl 4-(3, the 4-Dichlorobenzene base) piperazine-1-base
Also prepared following formula I chemical compound, wherein R is a methyl, R 1Be 2-(3, the 4-Dimethoxyphenyl) ethyl, R 2Be hydrogen, and X and Y are covalent bonds:
R 3Be 2, the 6-Dichlorobenzene base;
R 3It is 4-methylthiazol-2-base;
R 3Be 1,3-benzoxazole-2-base;
The 2-tolyl;
R 3It is the 2-chlorphenyl; With
R 3It is the 4-chlorphenyl.
D. the preparation of formula I chemical compound changes R 1 , R 2 , R 3 , R 4 , R 5 , X and Y
Similarly, method step according to top 4A, but use other formula (4) chemical compound to replace (9-{ (4S alternatively, 1R, 2R, 5R)-and 4-[(2-fluorobenzene sulfenyl) methyl]-7,7-dimethyl-3,6,8-trioxa-l-phosphabicyclo [3.3.0] suffering-2-yl } purine-6-yl) Aminocyclopentane, produced following formula I chemical compound.
Formula I chemical compound is replacedly produced with compound mode, as mentioned shown in the reaction scheme II.Example 5-8 describes this technology of use in detail and prepares individualized compound, and adopts this process to provide the parallel synthetic of a plurality of formula I chemical compounds with compound mode.
Example 5
The preparation of formula (5) chemical compound
A. the preparation of formula (5) chemical compound, wherein R 2 Be hydrogen
Figure BPA00001233335000681
(100g, 3.0mmol/g, 0.3mol, 150-300 μ m, Polymer Labs) is suspended in the exsiccant trimethyl orthoformate (1L) with P-benzyloxybenzaldehyde polystyrene resin (1).Add the p-methyl benzenesulfonic acid monohydrate (5.70g, 0.03mol, 0.1eq), and this suspension 48 hours of at room temperature vibrating.Add triethylamine (60mL), and filter resin rapidly, with the washed with dichloromethane that contains 1% triethylamine 4 times, and vacuum drying 24 hours, thereby obtain the dimethylacetal resin.
(20.0g, 3mmol/g 60.0mmol) are suspended in anhydrous N with the dimethylacetal resin, in the N-dimethyl acetylamide (300mL), and use nucleoside (34.4g, the 120mmol of formula (1) in succession, 2eq) (2.78g, 12.0mmol 0.2eq.) handle with the 10-camphorsulfonic acid.Under the room temperature under 200rpm vibration this mixture 96 hours.(4.2mL, 30.0mmol 0.5eq) and rapidly filter resin, wash once with N,N-dimethylacetamide, with containing 1%Et to add triethylamine then 3The dichloromethane of N and contain the MeOH alternate cycles washing four times of 1% triethylamine, and last with the washed with dichloromethane that contains 1% triethylamine three times.The resin that vacuum drying reclaims 48 hours, thereby the nucleoside of the resin-bonded of the formula of providing (5).
Example 6
The preparation of formula (6) chemical compound
A. the preparation of formula (6) chemical compound, wherein R and R 2 Be hydrogen, Y is a covalent bond, and R 1 It is cyclopenta
In reaction vessel, put into be suspended in anhydrous 1, nucleoside (the 30mg resin of the resin-bonded of the formula (5) that suspends in the 4-dioxane (30mL); Resin loads 1.5mmol/g).(2.4mL, 13.5mmol is 20eq) with excessive Aminocyclopentane to add diisopropylethylamine then.Under the situation that does not stir or do not stir in 80 ℃ of following reacting by heating containers 48 hours.After being cooled to room temperature, remove and desolvate, and adding containing the methanol of 1% triethylamine (50mL) with shrinkage resin.Product is with the MeOH that contains 1% triethylamine and contain the dichloromethane alternate cycles washing four times of 1% triethylamine, thus and the spend the night chemical compound of resin-bonded that formula (6) is provided of vacuum drying.
Example 7
The preparation of formula (7) chemical compound
A. the preparation of formula (7) chemical compound, wherein R and R 2 Be hydrogen, Y is a covalent bond, R 1 Be Cyclopenta, and R 3 It is the 2-fluorophenyl
Figure BPA00001233335000701
The product of example 6 is suspended in the anhydrous pyridine (2mL) also with diisopropylethylamine (0.13mL) processing.Be cooled to after 0 ℃, dropwise add mesyl chloride (0.035mL, 337mmol).During dripping, pass through hands stirred reaction mixture regularly.After 90 minutes, allow reactant mixture be raised to room temperature and vibrated 24 hours.After removing reactant mixture, wash this product with the anhydrous methylene chloride that contains 1% triethylamine, thus the methylsulfonyl derivant of the chemical compound of the formula of providing (6) resin-bonded.
(mesylate mesylate) is suspended in the acetonitrile (1.5mL) also with excessive diisopropylethylamine (0.16mL) processing and water (0.7mL) and 2-fluoro thiophenol (45mmol) processing subsequently with this methanesulfonates then.Under the condition of stirring this reaction vessel is not warmed up to nearly 80 ℃, is continuing 65 hours.Product is with the MeOH that contains 1% triethylamine and contain the dichloromethane alternate cycles washing four times of 1% triethylamine, and vacuum drying spends the night, thus the chemical compound of the formula of providing (7) resin-bonded.
Example 8
The preparation of formula I chemical compound
A. the preparation of formula I chemical compound, wherein R is a hydrogen, R 1 Be cyclopenta, R 2 Be hydrogen, R 3 Be The 2-fluorophenyl, and X and Y are covalent bonds
The chemical compound of the resin-bonded of formula (7) is suspended in the solution of 2% trifluoroacetic acid/5% ethanol/methylene and vibration (200rpm) at room temperature 2 hours.Remove after the solution, with dichloromethane rinse residue (3 * 0.5mL), and decompression concentrates the filtrate that merges down, thereby make the chemical compound (4S of formula I, 5S, 3R)-2-[6-(Aminocyclopentane base) purine-9-yl]-5-[(2-fluorobenzene sulfenyl) methyl] oxolane-3, the 4-glycol.
Example 9
The preparation of formula (9) chemical compound
Figure BPA00001233335000721
To the 6-chloropurine nucleoside (10.0g, 35mmol) in the solution in ethanol (350mL), add triethylamine (10.0mL, 100mmol) and (1R, 2R)-2-(benzyloxy)-Aminocyclopentane (5.2g, 52mmol).This mixture was refluxed 24 hours, and during this period, this reactant becomes settled solution by suspension.Remove ethanol under the decompression, and residue is distributed in ethyl acetate and water (100mL: 200mL).Separate organic layer and wash this organic layer twice (2 * 75mL) with ethyl acetate.The organic layer that dry (sodium sulfate) merges, and decompression is down except that desolvating.Residue is dissolved in the ethyl acetate (150mL), makes the product precipitation by adding hexane, thereby make 2-(6-{[(1R, 2R)-and 2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 3R, 5R)-5-(methylol) oxolane-3, the 4-glycol is white solid.
1HNMR(CD 3OD)δ1.62-2.16(m，6H)，3.26-3.29(m，1H，NHCH)，3.68-3.85(m，2H，CH 2-5′)，4.03-4.10(m，1H，CH-4′)，4.12-4.16(m，1H，CHOBn)，4.16-4.19(m，1H，3′CH)，4.71(s，2H，OCH 2Ph)，4.83-4.92(m，1H，2′CH)，5.98(d，J＝6Hz，1H，H-1′)，7.23-7.35(m，5H，PhH)，8.15(S，1H，C-2H)。
B. the preparation of formula (9) chemical compound
Similarly, according to the step of top 9A, but with other isomers of 2-(benzyloxy) Aminocyclopentane replace (1R 2R)-2-(benzyloxy)-Aminocyclopentane, has prepared following chemical compound:
2-(6-{[(1S, 2S)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 3R, 5R)-and 5-(methylol) oxolane-3, the 4-glycol;
2-(6-{[(1R, 2S)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 3R, 5R)-and 5-(methylol) oxolane-3, the 4-glycol;
2-(6-{[(1S, 2R)-2-(benzene first hydrogen base) cyclopenta] amino } purine-9-yl) (4S, 3R, 5R)-and 5-(methylol) oxolane-3, the 4-glycol;
2-(6-{[(1RS, 2RS)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 3R, 5R)-and 5-(methylol) oxolane-3, the 4-glycol.
Example 10
The preparation of formula (10) chemical compound
Figure BPA00001233335000731
Under 0 ℃, to the 2-(6-{[(1R that stirs, 2R)-and 2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 3R, 5R)-5-(methylol) oxolane-3,4-glycol (2.0g, 4.5mmol) acetonitrile (15mL) and pyridine (0.728mL, 9mmol) in the suspension, dropwise add thionyl chloride (1.7mL, 22.5mmol).After 0 ℃ is stirred 4 hours down, allow this reactant liquor be raised to room temperature, stir then and spend the night.The solvent in the gained suspension is removed in decompression down, thereby make 4-(6-{[(1R, 2R)-2-(benzyloxy) cyclopenta] amino purine-9-yl) (6S, 3aR, 6aR)-6-(chloromethyl)-4H, 6H, 3aH, 6aH-oxolane also [3,4-d] 1,3,2-sulphur dioxide heterocycle penta (dioxathiolan)-2-ketone, this material need not to be further purified and are directly used in next step.
B. the preparation of formula (10) chemical compound
Similarly, method step according to top 10A, but with 2-(6-{[2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 3R, 5R)-5-(methylol) oxolane-3, other isomers of 4-glycol replace 2-(6-{[(1R, 2R)-and 2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 3R, 5R)-5-(methylol) oxolane-3, the 4-glycol has prepared following compounds:
4-(6-{[(1S, 2S)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (6S, 3aR, 6aR)-and 6-(chloromethyl)-4H, 6H, 3aH, 6aH-oxolane be [3,4-d] 1,3 also, 2-two oxa-s Tetramethylene sulfide-2-ketone;
4-(6-{[(1R, 2S)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (6S, 3aR, 6aR)-and 6-(chloromethyl)-4H, 6H, 3aH, 6aH-oxolane be [3,4-d] 1,3 also, 2-sulphur dioxide heterocycle penta-2-ketone;
4-(6-{[(1S, 2R)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (6S, 3aR, 6aR)-and 6-(chloromethyl)-4H, 6H, 3aH, 6aH-oxolane be [3,4-d] 1,3 also, 2-sulphur dioxide heterocycle penta-2-ketone; With
4-(6-{[(1RS, 2RS)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (6S, 3aR, 6aR)-and 6-(chloromethyl)-4H, 6H, 3aH, 6aH-oxolane be [3,4-d] 1,3 also, 2-sulphur dioxide heterocycle penta-2-ketone.
Example 11
The preparation of formula (11) chemical compound
Will be from the 4-(6-{[(1R of example 10,2R)-and 2-(benzyloxy) cyclopenta] amino } purine-9-yl) (6S, 3aR, 6aR)-6-(chloromethyl)-4H, 6H, 3aH, 6aH-oxolane be [3,4-d] 1 also, 3,2-sulphur dioxide heterocycle penta-2-ketone is dissolved in the mixture of first alcohol and water (40mL/2mL), dropwise adds dense ammonium hydroxide (2.2mL, 28%) then in this solution.After 23 ℃ were stirred 30 minutes down, decompression removes down desolvated and water (15mL) dilution residue.(3 * 75mL) extraction mixture aqueous solutions are used MgSO with ethyl acetate 4Drying, and decompression removes down and desolvate, thus provide 2-(6-{[(1R, 2R)-2-(benzyloxy) cyclopenta] amino purine-9-yl) (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, the 4-glycol, it need not to be further purified and is directly used in next step.
B. the preparation of formula (11) chemical compound
Similarly, according to the method for top 11A, but with 4-(6-{[2-(benzyloxy) cyclopenta] amino } purine-9-yl) (6S, 3aR, 6aR)-and 6-(methyl)-4H, 6H, 3aH, 6aH-oxolane also [3,4-d] 1,3, other isomers of 2-sulphur dioxide heterocycle penta-2-ketone replace 4-(6-{[(1R, 2R)-and 2-(benzyloxy) cyclopenta] amino } purine-9-yl) (6S, 3aR, 6aR)-6-(chloromethyl)-4H, 6H, 3aH, the 6aH-oxolane is [3,4-d] 1,3 also, 2-sulphur dioxide heterocycle penta-2-ketone has made following compounds:
2-(6-{[(1S, 2S)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol;
2-(6-{[(1R, 2S)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol;
2-(6-{[(1S, 2R)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol; With
2-(6-{[(1RS, 2RS)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol.
Example 12
The preparation of formula (12) chemical compound
Figure BPA00001233335000761
The 2-that will in example 11, obtain (6-{[(1R, 2R)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3,4-glycol (22g) is dissolved in ethanol (450mL) and the bad hexane (200mL).In this solution, add palladium dydroxide (20mol% begins to add 1g, adds 1g after 6 hours, and adds 1g after 14 hours), and with this reaction mixture refluxed 18 hours.Pass through diatomite filtration while hot, and the solvent in the filtrate is removed in decompression down.Product grinds with ethanol (20mL), and filter, and use washing with alcohol, thereby make 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol is white powder.
The following recovery of other materials: the palladium dydroxide of regaining is dissolved in the methanol (200mL), and under 90 ℃, this mixture is heated up, continue 1 hour.By diatomite filtration should heat mixture, further wash this kieselguhr with hot methanol.Decompression is concentrated filtrate down, and residue grinds with ethanol (20mL), thereby makes the 2-{6-[((1R of other 8.6g, 2R)-2-hydroxycyclopent base) amino] purine-9 base } (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol.
1H?NMR(DMSO-d6)δ1.64-2.18(m，6H)，3.26-3.29(m，1H，NHCH)，3.83-3.97(m，2H，CH2C15′)，4.03-4.09(m，1H，CH-4′)，4.12-4.17(m，1H，CHOH)，4.16-4.19(m，1H，3′CH)，4.84-4.92(m，1H，2′CH)，5.96(d，J-6Hz，1H，H-1′)，7.23-7.35(m，5H，PhH)，8.15(S，1H，C-2H)，8.39(s，1H，C-8H)。
B. the preparation of formula (12) chemical compound
Similarly, method step according to top 12A, but with 2-(6-{[2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, other isomers of 4-glycol replace 2-(6-{[(1R, 2R)-and 2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, the 4-glycol has made following compounds:
2-(6-{[(1S, 2S)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol;
2-(6-{[(1R, 2S)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol;
2-(6-{[(1S, 2R)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol; With
2-(6-{[(1RS, 2RS)-2-(benzyloxy) cyclopenta] amino } purine-9-yl) (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol.
Example 13
The preparation of formula I chemical compound, wherein R is the 2-fluorophenyl
Figure BPA00001233335000781
To 2-fluoro thiophenol (38mL, 406mmol) in the solution in 2N sodium hydroxide (100mL), add 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3,4-glycol (15.0g, 40.6mmol) N, dinethylformamide (120mL) solution.This mixture is warmed up to 100 ℃, continues 4 hours, follow the tracks of the reaction progress with TLC.Remove N under the decompression, dinethylformamide, and water (200mL) dilution remaining mixture, with the acetic acid neutralization, (MgSO is used in 3 * 125mL) extractions with ethyl acetate 4The dry organic layer that merges.After decompression removed down and desolvates, residue ground and filters with diethyl ether, thereby made the 2-{6-[((1R of 16g, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorophenyl sulfenyl) methyl] oxolane-3, the 4-glycol is white powder.
1H?NMR(DMSO-d6)δ1.66-2.27(m，6H)，3.42-3.59(m，1H，NHC H)，4.05-4.14(m，2H)，4.03-4.09(m，1H，CH-4′)，4.14-4.19(m，1H)，4.16-4.19(m，1H，3′CH)，4.84-4.92(m，1H，2′CH)，5.97(d，J＝6Hz，1H，H-1′)，7.05-7.55(m，4H，PhH)，8.10(S，1H，C-2H)，8.15(s，1H，C-8H)。
B. the preparation of formula I chemical compound, wherein R is the 2-fluorophenyl
Similarly, method according to top 13A, but with 2-{6-[(-2-hydroxycyclopent base) amino] purine-9 base } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, other isomers of 4-glycol replace 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, the 4-glycol makes following compounds:
2-{6-[((1S, 2S)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl] oxolane-3, the 4-glycol;
2-{6-[((1R, 2S)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl] oxolane-3, the 4-glycol;
2-{6-[((1S, 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl] oxolane-3, the 4-glycol; With
2-{6-[((1RS, 2RS)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorophenyl sulfenyl) methyl] oxolane-3, the 4-glycol.
C. change the preparation of the formula I chemical compound of R
Similarly,, but replace the 2-fluoro thiophenol, prepared other formula I chemical compound with other phenylmercaptan .s of formula RSH according to the method for top 13A.
Example 14
The preparation of formula (19) chemical compound
Figure BPA00001233335000791
Preparation 1
In 55 fens clock times with thionyl chloride (SOCl 2, 66.0mL, (50.0g, 174.4mmol) (30mL is 370mmol) in cold (0 ℃, the ice bath) suspension at dry acetonitrile (600mL) and distilled pyridine 907mmol) dropwise to join the 6-chloropurine nucleoside.0 ℃ is stirred this reactant mixture down and stirred 18 hours under the room temperature then in 3 hours.At 40 ℃ of these yellow solutions of following concentrating under reduced pressure, under fine vacuum dry 6 hours then.Residue (6S, 4R, 3aR, 6aR)-6-(chloromethyl)-4-(6-chloropurine-9-yl)-4H, and 6H, 3aH, 6aH-oxolane be [3,4-d] 1,3 also, and 2-two oxa-s Tetramethylene sulfide-2-ketone (12) need not to be further purified and can be used for the next step reaction.
2. the replaceable preparation of formula (19) chemical compound
With thionyl chloride (SOCl 2, 530mL) dropwise be added in the mixture of 6-chloropurine nucleoside (1Kg) in the dry methylene chloride (15 liters) and distilled pyridine (850mL), in 30～60 minutes, temperature is remained on below 30 ℃.Stir this reactant mixture 4 hours down at 30 ℃, be cooled to 20 ℃ then.Add dehydrated alcohol (1700mL), temperature maintenance at 20 ℃, was stirred this mixture 15 minutes.Add water (3.5 liters) then lentamente, stirred this mixture 30 minutes, separable after this these inclusions.Phase-splitting, organic layer washs with 4 liters of saturated sodium bicarbonate.This biphase after separating, organic layer separates with 2.6 liters of saturated sodium chloride washings, and under reduced pressure reach nearly 4 liters until volume except that desolvating, (6S, 4R are provided, 3aR, 6aR)-6-(chloromethyl)-4-(6-chloropurine-9-yl)-4H, 6H, 3aH, the 6aH-oxolane is [3,4-d] 1,3 also, 2-sulphur dioxide heterocycle penta-2-ketone (12) solution, it need not to be further purified and can be used for the reaction of next step.
Example 15
The preparation of formula (20) chemical compound
Formula (19) compound dissolution that will obtain from example 14 (preparation 1) is methanol (1000mL) and distilled water (50mL).With this solution be cooled to 0 ℃ and in 25 minutes, dropwise add strong aqua ammonia (28%, 56mL).0 ℃ is continued down to stir 1 hour, stirred 3 hours under the room temperature then.During this period, add other 10mL strong aqua ammonia (28%) and (use TLC, CH 2Cl 2/ MeOH follows the tracks of reaction makes progress at 9: 1).This reactant mixture of concentrating under reduced pressure, residue are at room temperature used 5% aqueous citric acid solution (1000mL) hydrolysis.(1 * 900mL, 1 * 400mL, 1 * 200mL, 2 * 100mL) aqueous layer extracted, the organic layer of merging washs with saturated sodium bicarbonate (450mL) with ethyl acetate.(3 * 50mL) extract the sodium bicarbonate aqueous solution layer with ethyl acetate.The organic layer that merges washs with saline (400mL), and the sodium-chloride water solution layer is also used ethyl acetate (3 * 50mL) extractions.The organic layer dried over sodium sulfate that merges is filtered, thereby and formula (13) chemical compound of concentrating under reduced pressure filtrate acquisition 41.8g (4S, 5S, 2R, 3R)-5-(chloromethyl)-2-(6-chloropurine-9-yl) oxolane-3, the 4-glycol.Do not carry out further purification.
Preparation 2
Replacedly, ammonium hydroxide (500mL) is added in (6S, 4R, the 3aR that obtain in the preparation 2 of example 14,6aR)-6-(chloromethyl)-4-(6-chloropurine-9-yl)-4H, 6H, 3aH, 6aH-oxolane also [3,4-d] 1,3, in 2-sulphur dioxide heterocycle penta-2-ketone (12) solution, and under 25 ℃, stirred this mixture 12 hours.Filter solid, and wash with dichloromethane (500mL).Merging filtrate and cleaning mixture, vacuum reduces to about 6 liters with this volume.Be not further purified.
Example 16
The preparation of formula (18) chemical compound
Figure BPA00001233335000821
Preparation 1
With (4S, 5S, 2R, 3R)-5-(chloromethyl)-2-(6-chloropurine-9-yl) oxolane-3,4-glycol (36.3g, 118.7mmol) solution in the 400mL isopropyl alcohol dropwise adds (R, R)-(34.2g 249mmol) (uses calcium hydride exsiccant, 95mL at the isopropyl alcohol (100mL) and the distilled triethylamine of the degassing to the amino amylalcohol hydrochlorate of 2-, 69g is 226mmol) in the suspension in.Stirred this reactant mixture 30 minutes under the room temperature, backflow (oil bath temperature :～80 ℃) is 20 hours then.After the reactant mixture cool to room temperature, removal of solvent under reduced pressure, and with in the 1000mL water adding residue.Stir this suspension 3.5 hours under the room temperature, leach solid matter, and water (1 * 60mL and 1 * 90mL) washing, and use P under the vacuum 2O 5Dry 3 days, thus (81%) 2-{6-[((1R of generation 68.0g, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-(chloromethyl) oxolane-3, the 4-glycol is light brown powder.
Preparation 2
Replacedly, the solution that will obtain in the preparation 2 of example 15 is cooled to 20～25 ℃, adds triethylamine (1000mL), then add (R, R)-the amino amylalcohol (530g) of 2-.This mixture was refluxed 8 hours, under atmospheric pressure arrive about 4 liters until volume then except that desolvating.Mixture is cooled to 55～60 ℃, adds entry (15 liters), and mixture is cooled to 20～25 ℃.Stirred this mixture about 1 hour, and filtered then, with dehydrated alcohol (1.25 liters) washing solid, drying under reduced pressure solid, allowable temperature is not above 60 ℃.
B. similarly, method according to top 16A (preparation 1 or prepare 2), but with (S, S)-and the amino amylalcohol hydrochlorate replacement of 2-(R, R)-the amino amylalcohol hydrochlorate of 2-, make 2-{6-[((1S, 2S)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, the 4-glycol.
C. similarly, method according to top 16A (preparation 1 or prepare 2), but with (1R, 2S)-and the amino amylalcohol hydrochlorate replacement of 2-(R, R)-the amino amylalcohol hydrochlorate of 2-, make 2-{6-[((1R, 2S)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, the 4-glycol.
D. similarly, method according to top 16A (preparation 1 or prepare 2), but with (1S, 2R)-and the amino amylalcohol hydrochlorate replacement of 2-(R, R)-the amino amylalcohol hydrochlorate of 2-, make 2-{6-[((1S, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, the 4-glycol.
Example 17
The preparation of formula I chemical compound, wherein R is the 2-fluorophenyl
Figure BPA00001233335000831
Preparation 1
Per 2～3 hours will be with 2-fluoro thiophenol (190mL, 228g, the 1.78mol in the 38.5mL part, 4 equivalents) add 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3,4-glycol (166.5g, 0.457mol) and from the distillatory triethylamine of calcium hydride (352mL, 256g, 2.53mol, 4 equivalents) at the anhydrous N that outgases, in the solution in the dinethylformamide (1.8 liters).In this solution, blasted under the situation of nitrogen this mixture of stirring at room continuously 4 days (by 1The HNMR monitoring reaction).After reaction is finished, will pour in 7 liters of ethyl acetate with the washed reactant mixture of 3 premium on currency.(2 * 500mL) aqueous layer extracted, and the organic layer of water (3 * 2 liters) washing merging reduce to volume about 1.8 liters then, and the suspension of white solid is provided with ethyl acetate.Stirred this suspension 9 hours under the room temperature, leach white precipitate, with diethyl ether (3 * 200mL) washings, high vacuum dry 24 hours, thereby the 2-{6-[((1R of acquisition 131g (63% productive rate), 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol is white powder (93% purity).
1H?NMR(DMSO-d6)δ1.66-2.27(m，6H)，3.42-3.59(m，1H，NHC H)，4.05-4.14(m，2H)，4.03-4.09(m，1H，CH-4′)，4.14-4.19(m，1H)，4.16-4.19(m，1H，3′CH)，4.84-4.92(m，1H，2′CH)，5.97(d，J＝6Hz，1H，H-1′)，7.05-7.55(m，4H，PhH)，8.10(S，1H，C-2H)，8.15(s，1H，C-8H)。
Be further purified product by in 1 liter of ether (50: 1), stirring to spend the night, thereby obtain the pure 2-{6-[((1R of 127g, 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol.
Preparation 2
Product (1Kg) in the preparation 2 of example 16 is dissolved in the N,N-dimethylacetamide (2.7 liters), and adds potassium carbonate (560g).In this mixture that maintains below 25 ℃, add 2-fluoro thiophenol (380g), and heated this mixture about 6 hours down at 60～65 ℃.Then this mixture is cooled to 25～30 ℃, adds ethyl acetate (10 liters), then add water (4.9 liters) solution of sodium chloride (260g), and stirred this mixture 15 minutes.After with this two separate, organic facies is used water (4.9 liters) solution washing of sodium chloride (260g) once more, stirs this mixture 15 minutes.After separating, under atmospheric pressure concentrating organic layer to volume is about 5 liters, and adds methanol (10 liters).Under atmospheric pressure enriched mixture is cooled to about 35～40 ℃ to extremely about 2.8 liters of volumes with gained solution once more.Add dichloromethane (5 liters) then, mixture is maintained about 35～40 ℃, continue 1 hour, then be cooled to 0～5 ℃, continue 30 minutes.Leach solid product, with dichloromethane (2.8 liters) washing, drying under reduced pressure is to constant weight, allowable temperature does not surpass 50 ℃, thereby 2-{6-[((1R is provided, 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol.
By between 60～70 ℃ with 1Kg product 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol is dissolved in the methanol (20 liters) and is further purified this product, keeps this temperature about 1 hour, is cooled to 45～50 ℃, by 1 micron filter filtering solution, solution temperature is maintained more than 40 ℃ then.Solution temperature is maintained more than 40 ℃, concentrates this solution to about 7 liters, and with gained solution maintain 50～55 ℃ 1 hour.In 2 hours, this solution is cooled to-5 ℃ then, keep this temperature-5 ℃ 1 hour.Under-5 ℃, leach product, wash solid, thereby pure 2-{6-[((1R is provided with filter liquor, 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol.
B. the preparation of formula I chemical compound, wherein R is the stupid base of 2-fluorine
Similarly, method according to top 17A (step 1 or 2), but with 2-{6-[(2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, other isomers of 4-glycol replace 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-5-(chloromethyl) oxolane-3, the 4-glycol makes following compounds:
(2-{6-[((1S, 2S)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol);
(2-{6-[((1R, 2S)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol);
(2-{6-[((1S, 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol); With
(2-{6-[((1RS, 2RS)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl]-oxolane-3, the 4-glycol);
C. the preparation of formula I chemical compound changes R
Similarly,, but replace the 2-fluoro thiophenol, prepared other chemical compounds with other phenylmercaptan .s of formula RSH according to the method for top 17A (step 1 or 2).
Example 18
Prepared the hard capsule that contains following ingredients:
Figure BPA00001233335000861
Mix above composition and fill and enter in the hard capsule.
Example 19
Use following ingredients to prepare tablet:
Example 20
Preparation contains the dry powder inhaler formulations of following composition:
Composition Weight %
Active component 5
Lactose 95
Mix this active component and lactose and this mixture is added powder inhaler.
Example 21
Each preparation tablets that contains the 30mg active component is as follows:
Figure BPA00001233335000871
Active component, starch and cellulose are also fully mixed by 20# U.S. sieve (No.20 mesh U.S.sieve).The powder mixes of polyvinylpyrrolidonesolution solution and gained is passed through 16 order U.S. sieves (No.16 mesh U.S.sieve) with it then.The granule of so preparation is descended drying at 50 ℃～60 ℃, and it is passed through 16 order U.S. sieves.Previous carboxymethyl starch sodium, magnesium stearate and the Pulvis Talci by 30 order U.S. sieves joined in the granule, after the mixing, obtain the tablet that each weight is 120mg thereby on tablet machine, it is suppressed.
Example 22
Each suppository that contains the 25mg active component is prepared as follows:
Composition Amount
Active component 25mg
Saturated fatty acid glyceride 2,000mg
Active component is passed through 60 order U.S. sieves, and it is suspended in the saturated fatty acid glyceride that uses the previous fusing of required minimum heat.Then this mixture is poured in the suppository mould of specified 2.0g capacity and allowed its cooling.
Example 23
Each suspension that contains 50mg active component/5.0mL dosage is prepared as follows:
Composition Amount
Active component 50.0mg
Xanthan gum 4.0mg
Sodium carboxymethyl cellulose (11%)
Microcrystalline Cellulose (89%) 50.0mg
Sucrose 1.75g
Sodium benzoate 10.0mg
Spice and coloring agent q.v.
Pure water is to 5.0mL
Mixed active composition, sucrose and xanthan gum, and with it by 10 order U.S. sieves, then with the aqueous solution of the microcrystalline Cellulose and the sodium carboxymethyl cellulose of previous preparation.Under agitation add with a part of water dilution sodium benzoate, spice and coloring agent and with them.Thereby add enough water then and generate needed volume.
Example 24
Subcutaneous preparations can be prepared as follows:
Composition Amount
Active component 5.0mg
Semen Maydis oil 1.0mL
Example 25
Prepared ejection preparation with following composition:
Composition Amount
Active component 2.0mg/mL
Mannitol, USP 50mg/mL
Gluconic acid, USP q.s. (pH 5～6)
Water (distillation, aseptic) q.s. to 1.0mL
Nitrogen, NF q.s.
Example 26
Prepared topical formulations (topical preparation) with following composition:
Composition Gram
Active component 0.2-10
Sorbester p18 (Span 60) 2.0
Tween (tween) 60 2.0
Mineral oil 5.0
Vaseline (petrolatum) 0.10
Methyl hydroxybenzoate (methyl paraben) 0.15
Propylparaben (propyl paraben) 0.05
BHA (butylated hydroxyanisol) 0.01
Water q.s. to 100
Merge all the above components outside dewatering, and under agitation be heated to 60 ℃.The water that adds capacity then under 60 ℃ of vigorous stirring, thereby these compositions of emulsifying add the water of q.s. to 100g then.
Example 27
Slow releasing composition
Figure BPA00001233335000901
Slow release formulation of the present invention is prepared as follows: chemical compound and pH dependent binder and any optional excipient are closely mixed (do and mix).Then, granulation should be done the mixture that mixes under the situation that strong alkali aqueous solution exists, and strong alkali aqueous solution is injected in the blended powder.Particle drying, screening mixes with optional lubricant (for example Pulvis Talci or magnesium stearate), and it is pressed into tablet.Preferred strong alkali aqueous solution is an alkali hydroxide soln, for example sodium hydroxide or potassium hydroxide aqueous solution, preferred sodium hydrate aqueous solution (contain water-miscible solvent alternatively, as lower alcohol up to 25% 〉.
The tablet of gained can carry out coating with optional film former, the easness so that discriminating, taste masking purpose and improvement are swallowed.The amount of film former typically be tablet weight 2% and 4% between.Suitable film former is known in this area, and comprise hydroxypropyl emthylcellulose, cation methacrylate copolymer (dimethylaminoethyl methacrylate/methacrylic acid methyl-butyl ester copolymer-
Figure BPA00001233335000911
E-Rohm.Pharma) etc.These film former can contain coloring agent, plasticizer and other auxiliary element alternatively.
The tablet of compacting preferably has the hardness that is enough to bear the 8Kp compression.The big young pathbreaker of tablet depends primarily on the chemical compound amount in the tablet.Tablet will comprise the compound free alkali (compound free base) of 300～1100mg.Preferably, the scope of the amount that comprises of tablet is at the compound free alkali of 400-600mg, 650-850mg and 900-1100mg.
In order to influence rate of dissolution, the chemical compound that contains powder is carried out the time of wet mixing (wet mixed) and control.Preferably, the whole powder mixes time, promptly to be exposed to the time of sodium hydroxide solution be 1～10 minute to powder, is preferably 2～5 minutes.After granulation, granule is shifted out from granulating machine and is placed in the fluidized bed dryer, under about 60 ℃, to carry out drying.
Example 28
By the quantitative PCR analysis rna expression
According to the description of manufacturer, use the RNAeasy test kit (Qiagen, USA) and DNAse handle (Qiagen, USA), from cell, refrigerated tissue or be stored in the total RNA of separate tissue the RNALater (Qiagen).(ABI, Foster City CA) transcribe total RNA of 1 μ g to use random hexamer to utilize Taqman reverse transcription test kit in 50 μ L reaction.Diluted cDNA and in MX300xP QPCR system (Stratagene), used SYBR chemistry (Applied Biosystems, CA) enforcement QPCR with 1: 5.Use Beacon designer software (Premier Biosoft, CA) and the suitable sequential design of from the NCBI sequence library, introducing be used for the primer of rat and people ABCA-1 and rat and people's beta-actin.ABCA-1 expresses the beta-actin standardization.Use described mensuration, determine to handle influence ABCA-1 gene expression in the liver of ZDF (Zucker diabetes obesity) rat.0,2 and 4 hour formula I compound treatment rat with test.The result provides in Fig. 1.The formula I chemical compound of the test of using in Fig. 1 experiment is 2-{6-[((1R, 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl] oxolane-3, the 4-glycol.
Example 29
The measurement of ABCA-1 protein expression:
Come the expression of quantitative cell ABCA-1 albumen cell or tissue from homogenate, wherein this homogenate is to prepare from the tissue of homogenization during containing the lysis buffer of protease inhibitor or cell.Lysate albumen (lysate protein) separates by SDS-PAGE and carries out Western blotting, is used for ABCA1 and house keeping protein.Detect ABCA-1 with mouse monoclonal antibody with people, rat and mice cross reaction.With suitable commercialization antibody test beta-actin (or other normally used standardization albumen (normalizationprotein)).Use described mensuration, determine to handle influence liver ABCAI protein expression in the ZDF rat.0,2 and 4 hour formula I compound treatment rat with test.The results are shown among Fig. 2.This processing increases the ABCA-1 protein expression in time.The ABCA1 that some service time (time-point) vehicle Control is standardized in each time point expresses.The formula I chemical compound of the test of using in the experiment of Fig. 2 is 2-{6-[((1R, 2R)-2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(2-fluorobenzene sulfenyl) methyl] oxolane-3, the 4-glycol.
Example 30-expection
Determined the relation between ABCA-1 expression and the HDL level in lower body, measuring.
Feed by intraperitoneal injection or by 10%Cremaphore (BASF)/brinish filling, every day to 7 weeks big male C57B1/6 mices increase with predetermined close that external ABCA-1 expresses and be candidate compound pharmaceutically active and that can obtain in vivo.3～4 hours after the injection in the end, collect on an empty stomach edta plasma (fastedEDTA-plasma) and suitably tissue be used for analysis.By phosphotungstic acid precipitation (Sigma) separated plasma HDL, and use test kit (Roche Diagnostics) enzymatic assays HDL cholesterol, T-CHOL and triacylglycerol.Use two Superose 6/30 posts that cholesterol is connected in series in the elution fraction that detects with enzyme process, further analyzed the variation of HDL cholesterol and size by FPLC.RT-PCR by isolating RNA from candidate's tissue analyzes, and has further confirmed interior variation of body of ABCA-1 gene expression.
This mensuration is used for the relation between definite ABCA-1 expression and the HDL level.
Although the present invention is illustrated with reference to its specific embodiment,, it will be understood by those of skill in the art that under the situation that does not depart from true spirit of the present invention and scope, can make various changes and carry out the equivalence replacement.In addition, can carry out many modifications is so that compositions, technology, processing step or a plurality of processing step of concrete situation, material, material adapt to purpose of the present invention, spirit and scope.All such changes all fall within the scope of the appended claims.All patents above quoted and publication all are incorporated in this by reference.

Claims (21)

  1. One kind the treatment mammiferous morbid state method, described mammiferous morbid state can be by with can increasing the pharmaceutical treatment that ABCA-1 expresses and be alleviated, and described method comprises the chemical compound for the treatment of the formula I of effective dose to its mammal of needs:
    Figure FPA00001233334900011
    Formula I
    Wherein:
    R is hydrogen or low alkyl group;
    R 1Be the aryl of the cycloalkyl of the alkyl of optional replacement, optional replacement, optional replacement or the heteroaryl of optional replacement; Or
    R and YR 1Represent the heterocyclic radical of optional replacement with the nitrogen-atoms that links to each other with them;
    R 2Be hydrogen, halogen, trifluoromethyl, acyl group or cyano group;
    R 3Be the heteroaryl of the aryl of the cycloalkyl of optional replacement, optional replacement, optional replacement or the heterocyclic radical of optional replacement;
    R 4And R 5Be hydrogen or acyl group independently; And
    X and Y are the alkylidene of covalent bond or optional replacement independently;
    Precondition is to work as R 1Be methyl and Y when being covalent bond, when X is methylene or ethylidene, R 3Can not be phenyl.
  2. 2. method according to claim 1, wherein, R 3Be the aryl of optional replacement or the heteroaryl of optional replacement.
  3. 3. method according to claim 2, wherein, R, R 2, R 4And R 5All be hydrogen.
  4. 4. method according to claim 3, wherein, R 3It is the aryl of optional replacement.
  5. 5. method according to claim 4, wherein, R 1Be the cycloalkyl of optional replacement, X is a covalent bond, and R 3It is the phenyl of optional replacement.
  6. 6. method according to claim 5, wherein, Y is a covalent bond, R 1Be the cyclopenta of optional replacement and R 3By the phenyl of halogen or alkyl replacement.
  7. 7. method according to claim 6, wherein, R 1Be 2-hydroxycyclopent base and R 3Be the 2-fluorophenyl, promptly (4S, 5S, 2R, 3R)-and 5-[(2-fluorophenyl sulfenyl) methyl]-2-{6-[(2-hydroxycyclopent base) amino]-purine-9-yl } oxolane-3, the 4-glycol.
  8. 8. method according to claim 6, wherein, R 1Be 2-hydroxycyclopent base and R 3Be the 3-fluorophenyl, i.e. 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino]-purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(3-fluorophenyl sulfenyl) methyl] oxolane-3, the 4-glycol.
  9. 9. method according to claim 6, wherein, R 1Be 2-hydroxycyclopent base and R 3Be the 2-chlorphenyl, i.e. 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(2-chlorphenyl sulfenyl) methyl] oxolane-3, the 4-glycol.
  10. 10. method according to claim 6, wherein, R 1Be 2-hydroxycyclopent base and R 3Be the 2,4 difluorobenzene base, i.e. 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(2,4-difluorophenyl sulfenyl) methyl] oxolane-3, the 4-glycol.
  11. 11. method according to claim 6, wherein, R 1Be 2-hydroxycyclopent base and R 3Be the 4-chlorphenyl, i.e. 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(4-chlorphenyl sulfenyl) methyl] oxolane-3, the 4-glycol.
  12. 12. method according to claim 6, wherein, R 1Be 2-hydroxycyclopent base and R 3Be the 4-fluorophenyl, i.e. 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(4-fluorophenyl sulfenyl) methyl] oxolane-3, the 4-glycol.
  13. 13. method according to claim 6, wherein, R 1Be 2-hydroxycyclopent base and R 3Be 2, the 6-3,5-dimethylphenyl, i.e. 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(2,6-3,5-dimethylphenyl sulfenyl) methyl] oxolane-3, the 4-glycol.
  14. 14. method according to claim 6, wherein, R 1Be 2-hydroxycyclopent base and R 3Be the 2-tolyl, i.e. 2-{6-[((1R, 2R)-and 2-hydroxycyclopent base) amino] purine-9-yl } (4S, 5S, 2R, 3R)-and 5-[(2-aminomethyl phenyl sulfenyl) methyl] oxolane-3, the 4-glycol.
  15. 15. method according to claim 4, wherein, Y is the low-grade alkylidene of optional replacement, R 1And R 3All be the phenyl of optional replacement, and X is a covalent bond.
  16. 16. method according to claim 4, wherein, X and Y are covalent bonds, R 1Be the alkyl of optional replacement or the phenyl of optional replacement, and R 3It is the phenyl of optional replacement.
  17. 17. method according to claim 3, wherein, R 3It is the heteroaryl of optional replacement.
  18. 18. method according to claim 17, wherein, X and Y are covalent bonds, R 1Be the cycloalkyl of optional replacement, and R 3It is the 1,3-thiazoles-2-base of optional replacement.
  19. 19. method according to claim 17, wherein, Y is a low-grade alkylidene, R 1Be the cycloalkyl of optional replacement or the phenyl of optional replacement, and R 3It is the 1,3-thiazoles-2-base of optional replacement.
  20. 20. method according to claim 1, wherein, described morbid state is at least a disease that is selected from diabetes, insulin resistant, dyslipidemia, coronary artery disease and the inflammation.
  21. 21. method according to claim 20, wherein, described morbid state is a coronary artery disease.
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