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CN101892151B - Light controlled cell culture device - Google Patents

Light controlled cell culture device Download PDF

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Publication number
CN101892151B
CN101892151B CN 201010196313 CN201010196313A CN101892151B CN 101892151 B CN101892151 B CN 101892151B CN 201010196313 CN201010196313 CN 201010196313 CN 201010196313 A CN201010196313 A CN 201010196313A CN 101892151 B CN101892151 B CN 101892151B
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China
Prior art keywords
led
cell culture
drive circuit
control signal
culture device
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CN 201010196313
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CN101892151A (en
Inventor
屠洁
祁晖
王立平
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a light controlled cell culture device, comprising a singlechip, a first LED drive circuit, a second LED drive circuit, at least one first LED and at least one second LED, wherein the singlechip is used for receiving a wireless control instruction transmitted by a remote controller and processing the wireless control instruction to generate a first control signal or a second control signal; the first LED drive circuit is used for receiving the first control signal and controlling the work state of a first LED according to the first control signal; and the second LED drive circuit is used for receiving the second control signal and controlling the work state of a second LED according to the second control signal. The light controlled cell culture device has a light control function.

Description

Light controlled cell culture device
Technical field
The present invention relates to a kind of cell culture apparatus, relate in particular to a kind of light controlled cell culture device.
Background technology
The equipment (for example culturing bottle, culture dish, culture plate, chamber slide) that existing various types of cells is cultivated usefulness all can only satisfy simple cell cultures usefulness, does not have the light adjusting function.
Summary of the invention
In view of this, be necessary not have for traditional cell co-culture tool the problem of light adjusting function, a kind of light controlled cell culture device with light adjusting function is provided.
A kind of light controlled cell culture device, comprise micro-chip, the first led drive circuit, the second led drive circuit, at least one LED, at least one the 2nd LED, described micro-chip is used for receiving the controlled in wireless instruction of telepilot emission, and to controlled in wireless instruction is processed, produce the first control signal or the second control signal; Described the first led drive circuit is used for receiving the first control signal, and controls the working order of a LED according to the first control signal; Described the second led drive circuit is used for receiving the second control signal, and controls the working order of the 2nd LED according to the second control signal; Described device also comprises telepilot, and described telepilot is used for to the instruction of single chip microcomputers transmit controlled in wireless; Described device also comprises shell and led array plate, the setting of described led array plate in the enclosure, a described LED and the 2nd LED are arranged on the led array plate, described micro-chip, the first led drive circuit and the second led drive circuit are arranged on the surface of shell; Described led array plate comprises the array silver strip, and described array silver strip is used for fixing a LED and the 2nd LED; Described array silver strip also is used for as the wire that connects a LED and the 2nd LED.
Preferably, described controlled in wireless instruction comprises luminous intensity, the illumination frequency that increases or reduce a LED, the luminous intensity that increases or reduce the 2nd LED of opening or closing a LED, open or closing the 2nd LED, increase or reducing a LED, the illumination frequency that increases or reduce the 2nd LED.
Preferably, a described LED is blue-ray LED, and described the 2nd LED is yellow light LED.
Preferably, described led array plate also comprises the glass outer cover, and described glass outer cover is used for covering described array silver strip.
Preferably, also comprise display module, described display module is arranged on the surface of shell.
Preferably, also comprise Tissue Culture Plate, described Tissue Culture Plate setting and arranges with led array plate interval in the enclosure.
Preferably, at least one LED and at least one the 2nd LED are corresponding with a cell cultures hole on the Tissue Culture Plate on the described led array plate.
Preferably, a LED or the 2nd LED are corresponding with a cell cultures hole on the Tissue Culture Plate on the described led array plate.
Above-mentioned light controlled cell culture device is controlled the working order of a LED and the 2nd LED by micro-chip, the first led drive circuit, the second led drive circuit, thereby realizes the light adjusting function.
Description of drawings
Fig. 1 is the schematic diagram of light controlled cell culture device.
Fig. 2 is the schematic appearance of light controlled cell culture device.
Fig. 3 is the schematic diagram of the led array plate corresponding with 6 porocyte culture plates.
Fig. 4 is the schematic diagram of the led array plate corresponding with 12 porocyte culture plates.
Fig. 5 is the schematic diagram of the led array plate corresponding with 96 porocyte culture plates.
Embodiment
Fig. 1 is the schematic diagram of light controlled cell culture device.Light controlled cell culture device comprises telepilot, micro-chip, the first led drive circuit, the second led drive circuit, several LED, several the 2nd LED, telepilot is used for to the instruction of single chip microcomputers transmit controlled in wireless, micro-chip is used for to controlled in wireless instruction to be processed, and produces the first control signal or the second control signal; The first led drive circuit is used for controlling according to the first control signal the working order of a LED; The second led drive circuit is used for controlling according to the second control signal the working order of the 2nd LED.
The controlled in wireless instruction comprises luminous intensity, the illumination frequency that increases or reduce a LED, the luminous intensity that increases or reduce the 2nd LED of opening or closing a LED, open or closing the 2nd LED, increase or reducing a LED, the illumination frequency that increases or reduce the 2nd LED.
The one LED is blue-ray LED (wavelength is 460nm), and the 2nd LED is yellow light LED (wavelength is 590nm).
Fig. 2 is the schematic appearance of light controlled cell culture device.Light controlled cell culture device is except part above-mentioned, also comprise shell 10, Tissue Culture Plate 13, led array plate 15, led array plate 15 is arranged in the shell 10, the one LED and the 2nd LED are arranged on the led array plate 15, and micro-chip, the first led drive circuit and the second led drive circuit are arranged on the surface of shell 10.
Shell 10 is cubes or rectangular structure, is dark panel all around, without the panel of front one side, so that be placed on normal ventilation and replacing Tissue Culture Plate 13 and led array plate 15 in the common incubator.
Led array plate 15 is placed for extraction-type, can independently increase and decrease as required.
Be provided with the array silver strip on the led array plate 15, the array silver strip is used for fixing a LED and the 2nd LED, simultaneously also as the wire that connects a LED and the 2nd LED.Silver strip divides several specifications, LED that the above implants size, arrange different, with the time should select according to the size in the cell cultures hole on the Tissue Culture Plate of different size the led array silver strip of different size.
But there is the device of connecting or disconnecting circuit the both sides of led array plate 15, can link to each other with the electric wire buried underground on this both sides panel, and then be connected with the first led drive circuit and the second led drive circuit.
Also be provided with the glass outer cover on the led array plate 15, the glass outer cover is used for covering the array silver strip.The effect of glass outer cover is to prevent that silver strip from producing excessive temperature, thereby affects the normal life condition of device inner cell.
In addition, light controlled cell culture device also comprises display module 11 (for example LCD), and display module 11 is arranged on the surface of shell 10.Can intuitively manifest the related data parameter by display module 11.
Tissue Culture Plate 13 is that the interval arranges with led array plate 15, and the cell cultures hole on the LED on the led array plate 15 and the 2nd LED and the Tissue Culture Plate 13 has specific corresponding relation.
Fig. 3 is the schematic diagram of the led array plate corresponding with 6 porocyte culture plates.For 6 porocyte culture plates 31, two LED 321 and two the 2nd LED 322 on the led array plate 32 are corresponding with a cell cultures hole 310 on the 6 porocyte culture plates 31.
Fig. 4 is the schematic diagram of the led array plate corresponding with 12 porocyte culture plates.For 12 porocyte culture plates 41, a LED 421 and the 2nd LED 422 on the led array plate 42 are corresponding with a cell cultures hole 410 on the 12 porocyte culture plates 41.This corresponding relation also is applicable to 24 porocyte culture plates.
Fig. 5 is the schematic diagram of the led array plate corresponding with 96 porocyte culture plates.For 96 porocyte culture plates 51, a LED 521 or the 2nd LED 522 on the led array plate 52 are corresponding with a cell cultures hole 510 on the 96 porocyte culture plates 51.
Above-mentioned light controlled cell culture device can be used for common CO 2Incubator, this device can freely split as required, make up, outward appearance is dark, can prevent this device of light transmission and affects the function of other non-photosensitivity sense type cells incubator in, therefore can not affect the normal growth condition of interior other non-" photaesthesia type " cell of same incubator.This device can be used for studying the various physiological functions variations of cell under different frequency, wavelength laser stimulate of light sensation genetic modification, cooperates the effects such as various kinds of drug, cytokine, is further used for the clinical medicine research and development.
Above-mentioned light controlled cell culture device adopts yellow led or blue led to replace the LASER Light Source of respective wavelength, can realize cost advantage.
Above-mentioned light controlled cell culture device utilizes telepilot to control, and can pass through powered battery simultaneously, and therefore real realization is wireless, portable.
Above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Claims (8)

1. light controlled cell culture device, it is characterized in that: comprise micro-chip, the first led drive circuit, the second led drive circuit, at least one LED, at least one the 2nd LED, described micro-chip is used for receiving the controlled in wireless instruction of telepilot emission, and to controlled in wireless instruction is processed, produce the first control signal or the second control signal; Described the first led drive circuit is used for receiving the first control signal, and controls the working order of a LED according to the first control signal; Described the second led drive circuit is used for receiving the second control signal, and controls the working order of the 2nd LED according to the second control signal; Described device also comprises telepilot, and described telepilot is used for to the instruction of single chip microcomputers transmit controlled in wireless; Described device also comprises shell and led array plate, the setting of described led array plate in the enclosure, a described LED and the 2nd LED are arranged on the led array plate, described micro-chip, the first led drive circuit and the second led drive circuit are arranged on the surface of shell; Described led array plate comprises the array silver strip, and described array silver strip is used for fixing a LED and the 2nd LED; Described array silver strip also is used for as the wire that connects a LED and the 2nd LED.
2. light controlled cell culture device according to claim 1 is characterized in that: described controlled in wireless instruction comprises luminous intensity, the illumination frequency that increases or reduce a LED, the luminous intensity that increases or reduce the 2nd LED of opening or closing a LED, open or closing the 2nd LED, increase or reducing a LED, the illumination frequency that increases or reduce the 2nd LED.
3. light controlled cell culture device according to claim 1, it is characterized in that: a described LED is blue-ray LED, described the 2nd LED is yellow light LED.
4. light controlled cell culture device according to claim 1, it is characterized in that: described led array plate also comprises the glass outer cover, described glass outer cover is used for covering described array silver strip.
5. light controlled cell culture device according to claim 1, it is characterized in that: also comprise display module, described display module is arranged on the surface of shell.
6. light controlled cell culture device according to claim 1 is characterized in that: also comprise Tissue Culture Plate, described Tissue Culture Plate setting and arranges with led array plate interval in the enclosure.
7. light controlled cell culture device according to claim 6 is characterized in that: at least one LED and at least one the 2nd LED are corresponding with a cell cultures hole on the Tissue Culture Plate on the described led array plate.
8. light controlled cell culture device according to claim 6, it is characterized in that: a LED or the 2nd LED are corresponding with a cell cultures hole on the Tissue Culture Plate on the described led array plate.
CN 201010196313 2010-06-09 2010-06-09 Light controlled cell culture device Active CN101892151B (en)

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103074202B (en) * 2011-10-25 2014-10-08 中国科学院深圳先进技术研究院 In vitro array photo-stimulation system
CN103074210B (en) * 2013-01-25 2015-01-07 深圳先进技术研究院 Monitoring system for in-vitro cell photostimulation array device
CN109735450A (en) * 2019-03-11 2019-05-10 中国医学科学院生物医学工程研究所 A kind of low energy light source shelf for cell incubator

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2299443Y (en) * 1997-06-13 1998-12-09 中国农业大学 Thermostat culture box
DE202006003878U1 (en) * 2005-03-16 2006-06-14 Sartorius Ag Lighting device for cultivating phototropic cell cultures in incubators, comprises controllable light emitting diodes arranged on light elements, which forms a lighting matrix
CN201268702Y (en) * 2008-06-18 2009-07-08 中国科学技术大学 High evenness light irradiation biochemical cultivation case

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090303706A1 (en) * 2008-06-09 2009-12-10 Lucia Atehortua Wave length light optimizer for human driven biological processes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2299443Y (en) * 1997-06-13 1998-12-09 中国农业大学 Thermostat culture box
DE202006003878U1 (en) * 2005-03-16 2006-06-14 Sartorius Ag Lighting device for cultivating phototropic cell cultures in incubators, comprises controllable light emitting diodes arranged on light elements, which forms a lighting matrix
CN201268702Y (en) * 2008-06-18 2009-07-08 中国科学技术大学 High evenness light irradiation biochemical cultivation case

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
任克强.新型光照培养箱控制系统的设计与实现.《设计新苑》.2000,31,34. *
陈一立.光感基因神经调控技术的开发及对神经精神系统疾病的干预治疗.《互联网文章》.2009,1. *

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