CN101715894A - Fish protein oligopeptide for lefteye flounder - Google Patents
Fish protein oligopeptide for lefteye flounder Download PDFInfo
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- CN101715894A CN101715894A CN200910223443A CN200910223443A CN101715894A CN 101715894 A CN101715894 A CN 101715894A CN 200910223443 A CN200910223443 A CN 200910223443A CN 200910223443 A CN200910223443 A CN 200910223443A CN 101715894 A CN101715894 A CN 101715894A
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Abstract
The invention relates to fish protein oligopeptide for lefteye flounder, which is a compound feed additive for marine fish. The fish protein oligopeptide is made of wastes of codfish and is added into feed for lefteye flounder in a certain proportion to substitute protein in the feed, thereby enhancing the growth and survival rate of the lefteye flounder and the protein deposition and feed conversion rate, wherein the molecular weight of the fish protein oligopeptide is not more than 1000Da (not less than 85 percent). In addition, The fish protein oligopeptide with the optimal additive amount being 3.7 percent can obviously enhance the growth rate of lefteye flounder and the feed efficiency, increase the net utilization and deposition of protein and obviously enhance the protein contain of fish bodies.
Description
Technical field:
The invention belongs to the seawater fish additive for fodder, is a kind of growth accelerator that promotes the lefteye flounder growth.
Background technology:
For a long time owing to be extensive use of growth promotion health-care agents such as antibiotic, chemical synthetic drug, hormone in aquaculture and the aquatic feeds, the aquatic products Chinese traditional medicine is residual serious, not only directly be detrimental to health, and the decline of aquatic products quality, cause lacking the market competitiveness and prestige; Bacterial drug resistance strengthens, and antibody-resistant bacterium increases, and destroys ecological environment etc.Along with the be obstructed generation of incident of product water outlets such as eel, shrimp, the consumer is to problem growing interests such as the safety of food, health, health, and is more and more higher to the requirement of aquatic products quality.Accelerate development, the promotion and application new type of safe is feedstuff efficiently, and the safety and the quality that improve feed and food are extremely urgent.Theoretical according to modern times digestion, few skin can directly be absorbed by alimentary canal, and it is fast to have transport speed, consume energy low and is difficult for characteristics such as saturated, can eliminate with the absorption of free amino acid and compete, and has improved the absorption rate of albumen widely.Because it is very approaching that the composition of amino acid of fish albumen and human muscle form, the absorption rate height, thereby the exploitation fish protein oligopeptide causes people's extensive interest.But the research of at present relevant fish protein oligopeptide seldom, and the report that applies it in the aquatic feeds is very limited.Wang Jialin etc. (2006) substitute in the feed 12.6% protein with fish protein oligopeptide and are mixed with the forage feed perch, find that its specific growth rate and proteinosis rate are the highest.Liu Feng etc. (2006) substitute 25% fish meal protein with fish protein oligopeptide, make the throw something and feed postlarva of large yellow croaker of micro-granulated feed, can improve its survival rate significantly.
The Paralichthys Pleuronectiformes, the flounder suborder, Bothidae, Paralichthys are predacious fishes that dwell of a kind of end in the Pleuronectiformes, because tender, the delicious flavour, nutritious of its meat is loved by the people, the market demand is vigorous, economic worth is higher.The mixed feed of lefteye flounder mainly uses hard pellet diet and expanded pellet diet at present, exists palatability poor, and the speed of growth waits problem slowly.The growth accelerator of the environmental protection of relevant lefteye flounder mainly comprised enzyme preparation, probiotic, soyabean oligosaccharides etc. in the past, can be subjected to the influence of aspects such as cost, technology in the application process.Utilize the fish offal material to produce the growth accelerator of fish protein oligopeptide, both made full use of resource, reduced the consumption of fish meal again, can obviously promote the lefteye flounder growth, improved the utilization rate of protein as lefteye flounder.
Summary of the invention:
The objective of the invention is to substitute the protein in the feed,, improve proteins deposited rate and feed conversion rate to improve the growth and the survival rate of lefteye flounder by in the lefteye flounder feed, adding the fish protein oligopeptide of certain proportion, molecular weight ranges≤1000Da (〉=85%).
In order to solve existing problem in the background technology, the present invention by the following technical solutions: at first prepare fish protein oligopeptide.Get FF Pacific Ocean wall pollack leftover bits and pieces, be warming up to 10 ℃ of min sterilizations of 95 ℃ of insulations after the rubbing, add Alcalase protease and Flavourzyme protease, pH transferred to 8.5--9.0,55 ℃ of reactions 4 hours.Be warming up to 95 ℃ and be incubated 10min and carry out the protease deactivation then, coarse filtration is separated spur, centrifugal soluble protein oligopeptides water, insoluble protein precipitation and the fish oil of obtaining respectively of the further three-phase of enzymolysis liquid, soluble protein oligopeptides water is purified through 1KDa and the ultrafiltration of 5Ka milipore filter respectively, obtains the fish protein oligopeptide powder of different molecular weight ranges after the freeze drying.
Fish protein oligopeptide is added in the feed, and it is by with fish meal 21～30%, peanut meal 50%, high-strength flour 10～12.5%, choline 0.5%, lecithin 0.5%, calcium dihydrogen phosphate 1%, vitamin premix 1%, mineral substance premix 1%.After various raw materials are pressed the abundant mixing of consumption, add fish protein oligopeptide 0～6.0%, mixing once more, the coefficient of variation≤10% adds the fish oil of 60g/kg again, and fully mixing is made adhesive with 2% sodium alginate, the lefteye flounder of feeding after the granulation.
The present invention has following beneficial effect: utilize the leftover bits and pieces of fish to produce fish protein oligopeptide, change its nutritive effect, change thing leftover bits and pieces is the peptide albumen of biologically active, be a kind of aboundresources, peptide protein product of high quality and at a reasonable price, it not only deposits for nitrogenous source and utilization provides origin of amino acid, and contained little peptide can be absorbed rapidly, alleviated the competition that the intestines parietal cell is taken in different free amino acids, improves the combined coefficient of protein; The fish protein oligopeptide of development is added in the lefteye flounder mixed feed, not only can promote its growth, and improved the palatability of feed, improve food conversion ratio.
Description of drawings:
Fig. 1 is the schematic diagram that the present invention tests feed formula;
Fig. 2 is that the growth of experimental session lefteye flounder of the present invention reaches the schematic diagram to the utilization of feed;
Fig. 3 is the schematic diagram of lefteye flounder of the present invention to digestibility and the albumen net utilization and the deposition of four kinds of feeds;
Fig. 4 is the influence schematic diagrames of four kinds of feeds of the present invention to lefteye flounder fish body chemical composition.
The specific embodiment:
This specific embodiment is by the following technical solutions:
The making of fish protein oligopeptide: the leftover bits and pieces of FF Pacific Ocean wall pollack fish is dropped into enzyme reactor after meat grinder rubs, be warming up to 10 ℃ of min sterilizations of 95 ℃ of insulations, adding water and protease again mixes, material-water ratio 1: 1, adopt Alcalase enzyme E/S=90AU+Flavourzyme enzyme E/S=15000LAPU complex enzyme zymohydrolysis method, 55 ℃ of hydrolysis temperatures, initial pH=8.5, enzymolysis 3h, enzymolysis liquid heats up and makes the protease deactivation then, coarse filtration is separated spur, the centrifugal soluble protein oligopeptides water that obtains respectively of the further three-phase of enzymolysis liquid, insoluble protein precipitation and fish oil.Part soluble protein oligopeptides water is purified through the ultrafiltration of 1K milipore filter, obtain the protein oligopeptide powder after the spray-drying, molecular weight≤1000Da, another part soluble protein oligopeptides water is purified through the ultrafiltration of 5K milipore filter, obtain protein oligopeptide powder, molecular weight 1000-5000Da after the spray-drying.
The screening of fish protein oligopeptide for lefteye flounder: the primary raw material that will satisfy the lefteye flounder nutritional need earlier mixes, add the fish protein oligopeptide powder that above-mentioned two kinds of different molecular weights are formed then, adding proportion is 3%, mixing once more, the coefficient of variation≤10% adds the fish oil of 60g/kg again, fully mixing, sodium alginate with 2% is made adhesive, the lefteye flounder of feeding after the granulation.Through bimestrial growth experiment, when finishing, experiment, carries out Comprehensive Assessment by comparing the result of growth rate, survival rate and feed efficiency, find that the growth promotion effect of the fish protein oligopeptide of molecular weight≤1000Da is better than another kind of fish protein oligopeptide.
The selection of fish protein oligopeptide addition: by in the mixed feed of lefteye flounder, adding the fish protein oligopeptide of the molecular weight≤1000Da of different content, the feed formula of experiment is seen Fig. 1, make 4 groups of isonitrogenous diets, carried out 3 months feeding trial, when throwing something and feeding off-test, measure growing state, feed conversion situation and the protein utilization of lefteye flounder.
Referring to finding out among Fig. 2 that No. 3 feed gaining effects that add 3.7% fish protein oligopeptide in the lefteye flounder feed are the most obvious, be higher than other three groups significantly, but survival rate difference between each group is not remarkable.The feed coefficient that adds 3.7% fish protein oligopeptide group is minimum, significantly is lower than other three groups.The liver body is than do not have significant difference between each group.This shows that the addition of fish protein oligopeptide is 3.7%, can improve fish body weight gains rate and efficiency of feed utilization significantly.
Referring to Fig. 3 is digestibility and net protein utilization and the deposition of lefteye flounder to four kinds of experiment feeds.As can be seen from the table, adding fish protein oligopeptide lefteye flounder is improved the digestibility of feed, is 3.7% with addition wherein, and effect is the most obvious.Same add fish protein oligopeptide make lefteye flounder to the net protein utilization in the feed apparently higher than not interpolation group, the net protein utilization that adds 3.7% and 6% liang of group in the fish protein oligopeptide group is apparently higher than 1.2% group.The proteins deposited rate of lefteye flounder is the highest with 3.7% group, is significantly higher than other three groups.
Referring to Fig. 4, difference was not remarkable between the moisture of lefteye flounder, fat and content of ashes were respectively organized during off-test.Use to add the forage feed lefteye flounder of fish protein oligopeptide, its protein content is higher than and does not add group, does not add group but only there is 3.7% group body protein content to be significantly higher than.
The effect of No. 3 forage feed lefteye flounders of this specific embodiment is best, what it added fish protein oligopeptide consists of molecular weight≤1000Da (＞85%), optimum addition is 3.7% in the lefteye flounder feed, can improve growth rate, the feed efficiency of lefteye flounder significantly, increase the net utilization and the deposition of protein, significantly improve with macrura reevesii body protein content.
Claims (1)
1. fish protein oligopeptide for lefteye flounder, it is characterized in that fish protein oligopeptide is to utilize the leftover bits and pieces of cod to produce, in the lefteye flounder feed, add the fish protein oligopeptide of certain proportion, molecular weight ranges≤1000Da (〉=85%), substitute the protein in the feed, to improve the growth and the survival rate of lefteye flounder, improve proteins deposited rate and feed conversion rate, optimum addition is 3.7% in the lefteye flounder feed, can improve growth rate, the feed efficiency of lefteye flounder significantly, increase the net utilization and the deposition of protein, significantly improve with macrura reevesii body protein content.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910223443A CN101715894A (en) | 2009-11-16 | 2009-11-16 | Fish protein oligopeptide for lefteye flounder |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910223443A CN101715894A (en) | 2009-11-16 | 2009-11-16 | Fish protein oligopeptide for lefteye flounder |
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| CN101715894A true CN101715894A (en) | 2010-06-02 |
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| CN200910223443A Pending CN101715894A (en) | 2009-11-16 | 2009-11-16 | Fish protein oligopeptide for lefteye flounder |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689795A (en) * | 2015-11-13 | 2017-05-24 | 江苏银宝生物科技有限公司 | Feed formula for artificially breeding saltwater fish and processing method of feed formula |
| CN111454105A (en) * | 2020-04-23 | 2020-07-28 | 青岛海力源生物科技有限公司 | Fertilizer synergist containing fish protein oligopeptide and preparation method thereof |
-
2009
- 2009-11-16 CN CN200910223443A patent/CN101715894A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689795A (en) * | 2015-11-13 | 2017-05-24 | 江苏银宝生物科技有限公司 | Feed formula for artificially breeding saltwater fish and processing method of feed formula |
| CN111454105A (en) * | 2020-04-23 | 2020-07-28 | 青岛海力源生物科技有限公司 | Fertilizer synergist containing fish protein oligopeptide and preparation method thereof |
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Application publication date: 20100602 |