CN101703057B - Agricultural germicide composite containing famoxadone - Google Patents

Abstract

The invention discloses an agricultural germicide composite containing famoxadone and concretely discloses an agricultural germicide composite with the famoxadone as a first active ingredient and Methyram or propineb as a second active ingredient. In the composite, the weight ratio of the first active ingredient to the second active ingredient is 1:5-1:20, the accumulation quantity of the first active ingredient and the second active ingredient accounts for 10-80% of the total weight of the composite, preference for 40-80%. The germicide composite can be prepared into forms of water dispersible granules or wettable powder. The agricultural germicide composite has reasonable ingredients, treatment and protection actions, good germicidal effect, reduction of germicide-using times and low cost of germicide usage; the activity and the germicidal effect of the germicide composite are not simple superposition of activity of each ingredient, but have obvious synergy, and the germicide composite slows down resistance generation, is beneficial to safety of crops and conforms safety requirements of pesticide preparations.

Description

A kind of agricultural bactericidal composition of Han oxazole bacterium ketone

Technical field

The present invention relates to a kind of bactericidal composition, be specifically related to as the first active component De oxazole bacterium ketone and the bactericidal composition of forming as the Carbatene or the Propineb of second active component.

Background technology

The English common name famoxadone of oxazole bacterium ketone, molecular formula C ₂₂H ₁₈N ₂O ₄, chemical name 3-anilino--5-methyl-5-(4-Phenoxyphenyl)-1,3-azoles quinoline-2,4-diketone.Be a kind of new and effective, wide-spectrum bactericide, its mechanism of action is the mitochondria electron transfer inhibitor for the energy inhibitor; Cromoci oxidoreductase among the complex III there is inhibitory action.The bactericide inhibitory action comparative result that multiple inhibition is breathed is following: (IC50, ppb): JE874 (4.5); ICIA 5504 (170.0); BAS 490F (75.0); SSF-126 (3500.0).It has protection, treat, root out, infiltration, systemic activity, does not have the friendship resistance with the phenyl amide series bactericidal agent, suitable crop such as wheat, barley, pea, beet, rape, grape, potato, melon, capsicum, tomato, fruit tree etc.Be mainly used in the important disease of preventing and treating in Ascomycetes, Basidiomycetes, the oomycetes subclass such as powdery mildew, rust, glume blight, net blotch, downy mildew, late blight etc.It has lipophilicity, spray on the crop leaf after, be prone to adhere to, be difficult for by the rain drop erosion special efficacy, but its use cost is high, use separately to be difficult to large tracts of land and to promote.

The English common name metriam of Carbatene, two (the two thiocarbamate esters) zinc (2+) of chemical name three [ammonia] ethene)] [8-connects dithione for tetrahydrochysene-1,4,7-two thiophene diazocines-3] polymer.Carbatene is a kind of good protective fungicide, belongs to low-toxin farm chemicals.Because wide, the difficult resistance that produces of its bactericidal range, control efficiency obviously is superior to other same series bactericidal agents, so consumption is a large tonnage product in the world always.Be the substitute products of present other protective fungicides; Domestic most composite bactericide is all formulated with mancozeb processing; But the MnZn preparation can cause the crop trace element accumulation and poison; Through field application in recent years, remarkable to preventing and treating effects such as pear scab, alternaria leaf spot of apple, vegetable category eqpidemic disease, downy mildew, field crop rust.

The English common name propineb of Propineb, molecular formula (C ₅H ₈N ₂S ₄Zn) x, chemical name acrylic bisdithiocarbamic zinc.Propineb acts on the synthetic of fungal cell wall and protein, can suppress infecting and sprouting of spore, can suppress mycelial growth simultaneously, causes its distortion, death.And this medicine contains the zinc element that is easy to by the crop absorption, helps promoting plant growth and the quality that improves fruit.

Greengrocery, melon and fruit class, powdery mildew, downy mildew and anthracnose, early blight of tomato, apple marssonina leaf spot and the spot defoliation of grape, citrus scab, black spot of fruit tree are common diseases, usually become crushing disaster.In actual use ， oxazole bacterium ketone have protection, treat, root out, infiltration, systemic activity, the disease on the various plants is had very high bactericidal activity, but control exists pathogen that it is prone to produce resistance, the shortcoming that use cost is high separately.Carbatene, Propineb are difficult for producing resistance, but have only the protection prevention effect, no therapeutic action as a kind of broad spectrum protection property bactericide commonly used.

Summary of the invention

The object of the invention is to provide a kind of component reasonable, and bactericidal effect is good, and drug cost is low, the agricultural bactericidal composition that is difficult for developing immunity to drugs.

The purposes of powdery mildew, downy mildew and anthracnose, early blight of tomato, apple marssonina leaf spot and the spot defoliation that another purpose of the present invention is to provide above-mentioned composition to be used for preventing and treating greengrocery, melon and fruit class, grape, citrus scab, black spot of fruit tree.

In order to overcome the defective of existing single preparation, technical scheme of the present invention is to solve like this:

The present invention contain as the first active component De oxazole bacterium ketone with as the Carbatene or the Propineb of second active component, wherein the weight ratio of first active component and second active component is 1: 5～1: 20.The cumulant of first active component and second active component is 10%～80% of a said composition total weight, is preferably 40%～80%.

According to method known in those skilled in the art, can be mixed with agricultural during bactericidal composition practical application of the present invention and go up any formulation that allows, like water dispersible granules or wetting powder.

For water dispersible granules, those skilled in the art are familiar with the use corresponding adjuvants and accomplish the present invention very much.In dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate one or more; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; In disintegrant such as ammonium sulfate, urea, sucrose, the glucose one or more; In binding agent such as diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family one or more; In stabilizing agent such as methenamine, sodium citrate, the citric acid one or more; In filler such as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, the potter's clay one or more.

To wetting powder, spendable auxiliary agent has: one or more in dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; In stabilizing agent such as methenamine, sodium citrate, the citric acid one or more; In filler such as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, the potter's clay one or more.

Component of the present invention is reasonable, and treatment adds protective effect, and bactericidal effect is good; Reduce the medication number of times, drug cost is low, and its active and bactericidal effect is not the simple superposition of each composition activity; But significant synergistic effect is arranged; Slow down the generation of resistance, good to crop safety, meet the security requirement of pesticidal preparations.The present invention has the better prevention effect to greengrocery, melon and fruit class, powdery mildew, downy mildew and anthracnose, early blight of tomato, apple marssonina leaf spot and the spot defoliation of grape, citrus scab, black spot of fruit tree.

Embodiment

In order to prevent and treat diseases such as alternaria leaf spot of apple in the agricultural production, apple marssonina leaf spot, downy mildew of garpe, watermelon anthrax, citrus scab, watermelon powdery mildew, early blight of tomato, banana freckle, powdery mildew of cucumber; Inventor Yi oxazole bacterium ketone and Carbatene or Propineb composition have carried out mutual composite synergy research, and concrete grammar is:

1) the former medicine of alternaria leaf spot of apple bacterium (Alternariaalternataf.sp.mali) test medicament oxazole bacterium ketone adopts the former medicine of du pont company production; The former medicine that the former medicine of Carbatene adopts BASF European Co.,Ltd to produce, the former medicine that the former medicine of Propineb adopts Jiangsu favorable to the people chemical industry Co., Ltd to produce.Test is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy being provided with following 5 variable concentrations gradient: oxazole bacterium ketone respectively is 1.25 μ g/ml, 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml; Carbatene or Propineb are 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml; Oxazole bacterium ketone and Carbatene or the Propineb binary mixture concentration gradient that is mixed is 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml; If clear water contrast; Repeat 3 times.Adopt the mycelial growth rate method,, under aseptic condition, use the card punch of diameter, cut the bacterium cake, the bacterium cake is connected on the culture medium flat plate of different drug concentrations, put in 26 ℃ of incubators and cultivate 72h with transfer needle from colony edge as 5cm with cultured pathogen; The inspection colony diameter calculates the percentage that each chemicals treatment suppresses mycelial growth, and the EC of each medicament is obtained in the linear regression analysis between the probit value through inhibiting rate and the logarithm value of series concentration ₅₀Value, and calculate relative coefficient of synergism.

The Wadley method: estimate the synergistic effect of pharmacy mix according to coefficient of synergism (SR), both SR＜0.5 was an antagonism, and 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect.The coefficient of synergism of mixture (SR value) calculates by following formula:

$\mathrm{SR}=\frac{P_A+P_B}{P_A/A+P_B/B}\×100$

In the formula: X ₁--the EC of-mixture ₅₀Theoretical value, unit be the milligram every liter (mg/L)

P _A--the percentage composition of A in the-mixture, unit are percentage (%)

P _B--the percentage composition of B in the-mixture, unit are percentage (%)

The EC of A in the A---mixture ₅₀Value, unit is every liter (mg/L) of milligram

The EC of B in the B---mixture ₅₀Value, unit is every liter (mg/L) of milligram

$\mathrm{SR}=\frac{X_1}{X_2}........................$

In the formula:

The coefficient of synergism of SR---mixture;

X ₁---mixture EC ₅₀Theoretical value, unit is every liter (mg/L) of milligram;

X ₂---mixture EC ₅₀Measured value, unit are every liter (mg/L) of milligram.

Table 1 oxazole bacterium ketone and Carbatene, Propineb are mixed and make up the Toxicity Determination result to the alternaria leaf spot of apple bacterium

Medicament	Virulence regression equation	Correlation (r)	EC 50. (μg/ml)	EC 5095% confidence interval	Coefficient of synergism (SR)
						Oxazole bacterium ketone (A)	Y＝3.8314+1.4862X	0.9989	6.11	5.1526～ 7.4583	-
Carbatene (B)	Y＝1.6633+1.5493X	0.9995	142.48	106.88～ 196.85	-
						Propineb (C)	Y＝1.7143+1.4431X	0.9979	189.15	106.28～ 392.76	-
A+B (1∶1)	Y＝3.3386+1.5943X	0.9957	11.02	7.5991～ 18.1724	- 1.06
						A+B (1∶2.5)	Y＝3.2423+1.5593X	0.9929	13.40	8.0269～ 29.0660	1.44
A+B (1∶5)	Y＝3.0519+1.5485X	0.9979	18.12	12.8537～ 27.9486	1.67

A+B (1∶10)	Y＝2.9333+1.5403X	0.9995	21.97	18.0800～ 27.3500	2.14
						A+B (1∶20)	Y＝2.8081+1.3680X	0.9992	40.02	30.4926～ 54.8200	1.73
A+B (1∶40)	Y＝2.6556+1.3276X	0.9983	58.34	51.0761～ 67.2202	1.57
						A+B (1∶80)	Y＝2.3885+1.3485X	0.9973	86.43	53.1400～ 159.5200	1.29
A+C (1∶1)	Y＝3.5754+1.4966X	0.9963	8.95	6.5657～ 13.3742	1.32
						A+C (1∶2.5)	Y＝3.4616+1.4091X	0.9933	12.35	7.5418～ 25.8657	1.60
A+C (1∶5)	Y＝3.1888+1.4024X	0.9941	19.56	11.1357～ 45.6684	1.61
						A+C (1∶10)	Y＝2.7996+1.5152X	0.9984	28.33	19.9151～ 43.7883	1.79
A+C (1∶20)	Y＝2.3964+1.4106X	0.9989	70.11	50.4542～ 103.0267	1.56
						A+C (1∶40)	Y＝2.1172+1.3979X	0.9998	115.42	102.06～ 131.37	1.19
A+C (1∶80)	Y＝2.5752+1.4668X	0.9998	144.99	38.9842～ 52.4787	1.03

2) marssonina coronaria (Marssonina mali (P.Henn) Ito) test method is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first with single agent and each Mixed Pharmacy be provided with respectively following 5 variable concentrations gradient: oxazole bacterium ketone be 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml, 40 μ g/ml; Carbatene or Propineb are 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml; Oxazole bacterium ketone and Carbatene or the Propineb binary mixture concentration gradient that is mixed is 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml; If clear water contrast; Repeat 3 times.Adopt the mycelial growth rate method to obtain the EC of each medicament ₅₀Value, and calculate relative coefficient of synergism.

Table 2 oxazole bacterium ketone and Carbatene, Propineb are mixed and make up the Toxicity Determination result to marssonina coronaria

Medicament	Virulence regression equation	Correlation (r)	EC 50. (μg/ml)	EC 5095% confidence interval	Coefficient of synergism SR)
						Oxazole bacterium ketone (A)	Y＝3.2708+1.5056X	0.9977	14.08	10.1306～ 21.3979	-
Carbatene (B)	Y＝2.0841+1.4043X	0.9979	119.22	68.4480～ 243.06	-
						Propineb (C)	Y＝2.3502+1.2137X	0.9995	152.47	112.4313～ 215.2066	-
A+B (1∶1)	Y＝3.1867+1.4198X	0.9964	18.93	12.0562～ 35.1410	1.33
						A+B (1∶2.5)	Y＝2.9714+1.4829X	0.9989	23.33	17.8351～ 32.0475	1.63
A+B (1∶5)	Y＝3.0058+1.3519X	0.9991	29.86	22.5985～ 41.4425	1.78
						A+B (1∶10)	Y＝2.7515+1.4783X	0.9997	33.18	27.8000～ 40.3346	2.14
A+B (1∶20)	Y＝2.6372+1.3875X	0.9998	50.45	43.6207～ 58.9908	1.74
						A+B (1∶40)	Y＝2.4295+1.4084X	0.9993	66.86	51.3042～ 90.2730	1.51
A+B (1∶80)	Y＝2.3583+1.4016X	0.9984	76.71	52.3261～ 121.3436	1.42
						A+C (1∶1)	Y＝3.1917+1.3252X	0.9992	23.14	18.2162～ 30.5534	1.11
A+C (1∶2.5)	Y＝2.8941+1.5036X	0.9966	25.15	15.6611～ 47.8977	1.13
						A+C (1∶5)	Y＝2.6423+1.5218X	0.9986	35.43	24.9999～ 54.1722	1.63
A+C (1∶10)	Y＝2.7037+1.4631X	0.9975	37.11	23.5537～ 67.0255	2.17
						A+C (1∶20)	Y＝2.5303+1.4011X	0.9998	57.90	50.5771～ 66.8848	1.79
A+C (1∶40)	Y＝2.4643+1.3301X	0.9992	80.61	61.3624～ 109.7969	1.53
						A+C (1∶80)	Y＝2.2631+1.3777X	0.9993	96.97	75.0109～ 129.2600	1.40

3) downy mildew of garpe bacterium (Plasmopara viticola) test method is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy being provided with following 5 variable concentrations gradient: oxazole bacterium ketone respectively is 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml, 40 μ g/ml; Carbatene, Propineb are 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml; Oxazole bacterium ketone and Carbatene or the Propineb binary mixture concentration gradient that is mixed is 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml; If clear water contrast; Repeat 3 times.Adopt the mycelial growth rate method to obtain the EC of each medicament ₅₀Value, and calculate relative coefficient of synergism.

Table 3 oxazole bacterium ketone and Carbatene, Propineb are mixed and make up the Toxicity Determination result to downy mildew of garpe

Medicament	Virulence regression equation	Correlation (r)	EC 50. (μg/ml)	EC 5095% confidence interval	Coefficient of synergism SR)
						Oxazole bacterium ketone (A)	Y＝3.2555+1.3524X	0.9998	19.49	17.3167～ 22.1475	-
Carbatene (B)	Y＝1.8443+1.3806X	0.9988	193.10	125.1624～ 323.2077	-
						Propineb (C)	Y＝1.8125+1.3299X	0.9987	249.36	155.1877～ 440.2210	-
A+B (1∶1)	Y＝2.9008+1.4154X	0.9975	30.42	19.9047～ 52.6113	1.16
						A+B (1∶2.5)	Y＝2.5682+1.5053X	0.9989	41.26	29.8136～ 60.7657	1.32
A+B (1∶5)	Y＝2.7051+1.3484X	0.9989	50.34	35.7739～ 75.6818	1.54
						A+B (1∶10)	Y＝2.6374+1.4287X	0.9988	45.05	32.0083～ 67.8732	2.37
A+B (1∶20)	Y＝2.1966+1.5119X	0.9982	71.47	45.1877～ 126.9614	1.89
						A+B (1∶40)	Y＝2.1323+1.4257X	0.9995	102.65	80.0357～ 135.4259	1.55
A+B (1∶80)	Y＝1.7754+1.4850X	0.9983	148.39	94.9613～ 254.1769	1.17

A+C (1∶1)	Y＝2.5052+1.6597X	0.9966	31.86	19.6572～ 60.8906	1.13
						A+C (1∶2.5)	Y＝2.5371+1.5221X	0.9993	41.51	31.7835～ 56.4556	1.39
A+C (1∶5)	Y＝2.5754+1.3925X	0.9988	55.10	38.0085～ 86.3380	1.53
						A+C (1∶10)	Y＝2.4853+1.4241X	0.9968	58.32	32.9907～ 126.1933	2.06
A+C (1∶20)	Y＝2.4291+1.3359X	0.9994	84.02	62.7683～ 117.2208	1.88
						A+C (1∶40)	Y＝2.1419+1.3464X	0.9988	132.66	88.8976～ 213.2033	1.46
A+C (1∶80)	Y＝1.8446+1.3857X	0.9992	189.33	139.6904～ 266.5782	1.16

4) watermelon anthrax bacteria (colletotrichum orbiculare) test method is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy being provided with following 5 variable concentrations gradient: oxazole bacterium ketone respectively is 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml, 40 μ g/ml; Carbatene, Propineb are 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml; Oxazole bacterium ketone and Carbatene or the Propineb binary mixture concentration gradient that is mixed is 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, establishes the clear water contrast; Repeat 3 times.Adopt the mycelial growth rate method to obtain the EC of each medicament ₅₀Value, and calculate relative coefficient of synergism.

Table 4 oxazole bacterium ketone and Carbatene, Propineb are mixed and make up the Toxicity Determination result to watermelon anthrax

Medicament	Virulence regression equation	Correlation (r)	EC 50 (μg/ml)	EC 5095% confidence interval	Coefficient of synergism (SR)
						Oxazole bacterium ketone (A)	Y＝3.6980+1.4091X	0.9991	8.39	7.0054～ 10.3504	-
Carbatene (B)	Y＝2.1194+1.3758X	0.9994	124.12	91.1389～ 176.4657	-
						Propineb (C)	Y＝2.1459+1.3137X	0.9995	148.79	109.0700～ 211.6658	-

A+B (1∶1)	Y＝2.9559+1.6545X	0.9942	17.19	10.2701～ 36.5925	0.91
						A+B (1∶2.5)	Y＝3.1345+1.5474X	0.9986	16.05	12.4349～ 21.7591	1.57
A+B (1∶5)	Y＝3.1091+1.3978X	0.9959	22.53	13.6839～ 45.2924	1.67
						A+B (1∶10)	Y＝3.0030+1.3977X	0.9981	26.84	18.3936～ 43.2353	2.05
A+B (1∶20)	Y＝2.6775+1.4464X	0.9972	40.34	25.0687～ 75.2514	1.86
						A+B (1∶40)	Y＝2.6881+1.2929X	0.9994	61.40	48.1235～ 80.7559	1.51
A+B (1∶80)	Y＝2.6319+1.2025X	0.9974	93.17	56.0492～ 174.5922	1.14
						A+C (1∶1)	Y＝3.3167+1.4175X	0.9976	15.40	10.9081～ 23.9437	1.03
A+C (1∶2.5)	Y＝3.3050+1.3789X	0.9983	16.95	12.4443～ 24.8295	1.52
						A+C (1∶5)	Y＝2.8634+1.5371X	0.9976	24.55	16.4806～ 41.0107	1.60
A+C (1∶10)	Y＝2.7266+1.5358X	0.9993	30.22	23.7195～ 39.9178	1.95
						A+C (1∶20)	Y＝2.4455+1.5237X	0.9995	47.49	38.0859～ 60.7761	1.74
A+C (1∶40)	Y＝2.5616+1.3289X	0.9995	68.35	54.4772～ 87.9785	1.55
						A+C (1∶80)	Y＝2.4783+1.2561X	0.9973	101.76	62.1550～ 188.7966	1.21

5) citrus scab bacterium (Sphaceloma fawcettii Jenkins) test method is with reference to " the agricultural industry criteria NY/T1156.3-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy being provided with following 5 variable concentrations gradient: oxazole bacterium ketone respectively is 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml, 40 μ g/ml; It is 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml that Carbatene, Propineb are; Oxazole bacterium ketone and Carbatene or the Propineb binary mixture concentration gradient that is mixed is 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, establishes the clear water contrast; Repeat 3 times.Adopt the mycelial growth rate method to obtain the EC of each medicament ₅₀Value, and calculate relative coefficient of synergism.

Table 5 oxazole bacterium ketone and Carbatene, Propineb are mixed and make up the Toxicity Determination result to citrus scab

Medicament	Virulence regression equation	Correlation (r)	EC 50. (μg/ml)	EC 5095% confidence interval	Coefficient of synergism (SR)
						Oxazole bacterium ketone (A)	Y＝2.9347+2.2651X	0.9940	9.37	5.7162～ 18.1456	-
Carbatene (B)	Y＝0.4234+2.1710X	0.9970	147.03	58.0846～ 558.5270	-
						Propineb (C)	Y＝1.0052+1.3137X	0.9996	148.79	52.2341～ 351.7527	-
A+B (1∶1)	Y＝2.5770+1.8729X	0.9964	22.76	11.7023～ 59.8625	0.77
						A+B (1∶2.5)	Y＝2.2174+1.6632X	0.9920	21.84	9.9406～ 83.4171	1.29
A+B (1∶5)	Y＝3.1075+1.4998X	0.9930	21.44	9.9024～ 78.3719	1.99
						A+B (1∶10)	Y＝3.0901+1.5687X	0.9992	19.31	11.0549～ 39.9650	3.26
A+B (1∶20)	Y＝2.4975+1.7699X	0.9903	30.11	12.3218～ 150.1414	2.87
						A+B (1∶40)	Y＝2.5530+1.5184X	0.9997	47.88	24.5751～ 114.7252	2.26
A+B (1∶80)	Y＝2.5647+1.3632X	0.9956	74.61	31.5223～ 266.9543	1.67
						A+C (1∶1)	Y＝2.8804+1.7170X	0.9986	19.96	11.2095～ 43.0152	0.88
A+C (1∶2.5)	Y＝3.1363+1.3740X	0.9900	26.81	10.8327～ 147.5280	1.06
						A+C (1∶5)	Y＝2.1334+1.9851X	0.9977	27.80	18.9192～ 45.2347	1.54
A+C (1∶10)	Y＝1.9074+1.9652X	0.9987	37.47	27.7071～ 54.3268	1.69

A+C (1∶20)	Y＝2.1173+1.7188X	0.9995	47.55	43.8655～ 51.7028	1.83
						A+C (1∶40)	Y＝1.8081+1.7272X	0.9961	70.47	37.9539～ 165.1447	1.55
A+C (1∶80)	Y＝2.5405+1.2296X	0.9998	118.92	58.7261～ 285.1162	1.06

6) tomato early blight bacterium (Alternaria solani) test method is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy being provided with following 5 variable concentrations gradient: oxazole bacterium ketone respectively is 1.25 μ g/ml, 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml; Carbatene, Propineb are 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml; Oxazole bacterium ketone and Carbatene or the Propineb binary mixture concentration gradient that is mixed is 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, establishes the clear water contrast; Repeat 3 times.Adopt the mycelial growth rate method to obtain the EC of each medicament ₅₀Value, and calculate relative coefficient of synergism.

Table 6 oxazole bacterium ketone and Carbatene, Propineb are mixed and make up the Toxicity Determination result to early blight of tomato

Medicament	Virulence regression equation	Correlation (r)	EC 50. (μg/ml)	EC 5095% confidence interval	Coefficient of synergism (SR)
						Oxazole bacterium ketone (A)	Y＝3.7384+1.7787X	0.9967	5.95	3.8651～ 10.4008	-
Carbatene (B)	Y＝1.4344+1.7856X	0.9993	115.02	49.5768～ 368.1006	-
						Propineb (C)	Y＝1.3494+1.8479X	0.9988	109.28	46.3422～ 365.5917	-
A+B (1∶1)	Y＝3.3490+1.5540X	0.9913	13.52	6.9088～ 40.3443	0.84
						A+B (1∶2.5)	Y＝2.8168+1.8247X	0.9919	18.22	8.8696～ 58.8213	1.01
A+B (1∶5)	Y＝2.8486+1.7963X	0.9983	18.29	10.4574～ 38.2375	1.55
						A+B (1∶10)	Y＝2.9308+1.7487X	0.9958	17.72	9.4734～ 43.8790	2.43

A+B (1∶20)	Y＝2.7952+1.8148X	0.9982	19.02	10.7406～ 40.7295	3.23
						A+B (1∶40)	Y＝2.7643+1.2889X	0.9992	64.30	30.7847～ 173.8757	1.24
A+B (1∶80)	Y＝2.6835+1.2315X	0.9989	90.40	41.8082～ 254.1404	1.03
						A+C (1∶1)	Y＝2.7842+1.9849X	0.9990	15.09	9.4068～ 26.6359	0.74
A+C (1∶2.5)	Y2.7018+2.0135X	0.9992	15.97	9.9439～ 28.1555	1.15
						A+C (1∶5)	Y＝2.5919+2.0381X	0.9992	17.51	10.6388～ 32.1985	1.60
A+C (1∶10)	Y＝2.3540+1.5012X	0.9962	18.66	10.2042～ 43.2506	2.27
						A+C (1∶20)	Y＝2.6066+1.9385X	0.9983	19.84	11.2371～ 41.9463	3.01
A+C (1∶40)	Y＝2.0234+1.8063X	0.9996	51.50	27.0494～ 116.7349	1.49
						A+C (1∶80)	Y＝1.8306+1.8070X	0.9942	65.74	28.7370～ 220.1715	1.37

7) banana freckle (Macrophoma muscle) test method is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy being provided with following 5 variable concentrations gradient: oxazole bacterium ketone respectively is 1.25 μ g/ml, 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml; Carbatene, Propineb are 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml; Oxazole bacterium ketone and Carbatene or the Propineb binary mixture concentration gradient that is mixed is 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, establishes the clear water contrast; Repeat 3 times.Adopt spore suspension to sprout method, with deionized water wash-out, filtration from the medium, centrifugal (1000r/min) 5min removes supernatant, adds deionized water, and is centrifugal again with cultured pathogen spore.At last with deionized water with the spore resuspension to every milliliter 1 * 10 ⁵Individual～1 * 10 ⁷Individual spore, and add 0.5% glucose solution., from the low concentration to the high concentration, draw soup 0.5mL successively and add respectively in the small test tube with pipette, draw the spore suspension 0.5mL for preparing then, soup and spore suspension mixed in equal amounts are shaken up; Getting above-mentioned mixed liquor with micro sample adding appliance drips on the concave slide; Frame is put in the culture dish that has shoaling layer then; Adding a cover preserves moisture is incubated in the incubator of preference temperature, and when the blank spore germination rate reached 90%, the spore germination situation was respectively handled in inspection; Calculate and suppress preventive effect relatively, obtain the EC of each medicament ₅₀Value, and calculate relative coefficient of synergism.

Table 7 oxazole bacterium ketone and Carbatene, Propineb are mixed and make up the Toxicity Determination result to banana freckle

Medicament	Virulence regression equation	Correlation (r)	EC 50 (μg/ml)	EC 5095% confidence interval	Coefficient of synergism (SR)
						Oxazole bacterium ketone (A)	Y＝3.3784+1.8910X	0.9970	8.34	5.2878～ 14.9474	-
Carbatene (B)	Y＝1.5639+1.7141X	0.9992	117.35	49.4743～ 394.1601	-
						Propineb (C)	Y＝1.6009+1.7110X	0.9988	112.60	46.8554～ 392.3254	-
A+B (1∶1)	Y＝2.9072+1.7659X	0.9932	17.79	8.8802～ 53.3458	0.88
						A+B (1∶2.5)	Y＝2.8515+1.7926X	0.9937	18.33	9.2041～ 53.6929	1.35
A+B (1∶5)	Y＝2.7448+1.7642X	0.9989	22.04	12.2873～ 47.7821	1.68
						A+B (1∶10)	Y＝2.8581+1.7402X	0.9991	19.78	11.3852～ 40.3818	2.71
A+B (1∶20)	Y＝2.4572+1.9292X	0.9980	24.03	12.9084～ 56.2319	3.01
						A+B (1∶40)	Y＝1.9484+1.9693X	0.9999	40.89	22.2493～ 87.9191	2.18
A+B (1∶80)	Y＝2.7035+1.2695X	0.9908	76.40	27.1046～ 454.1632	1.32
						A+C (1∶1)	Y＝3.4121+1.4445X	0.9915	14.79	7.3818～ 46.4919	1.05

A+C (1∶2.5)	Y＝3.2215+1.6333X	0.9954	14.32	7.9185～ 33.5962	1.72
						A+C (1∶5)	Y＝3.2890+1.5594X	0.9911	14.64	7.2924～ 46.4999	2.49
A+C (1∶10)	Y＝3.3389+1.4816X	0.9929	15.53	7.8204～ 46.8292	3.39
						A+C (1∶20)	Y＝2.8612+1.4961X	0.9996	31.54	16.7158～ 73.6169	2.24
A+C (1∶40)	Y＝2.5272+1.5076X	0.9980	51.17	24.2441～ 145.9674	1.69
						A+C (1∶80)	Y＝2.7165+1.2810X	0.9995	71.84	34.6340～ 189.0030	1.36

8) powdery mildew of cucumber bacterium (Sphaerotheca fuliginea (Schlecht) Poll) test method is with reference to " the agricultural industry criteria NY/T1156.1-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy being provided with following 5 variable concentrations gradient: oxazole bacterium ketone respectively is 1.25 μ g/ml, 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml; Carbatene, Propineb are 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml; Oxazole bacterium ketone and Carbatene or the Propineb binary mixture concentration gradient that is mixed is 6.25 μ g/ml, 12.5 μ g/ml, 25 μ g/ml, 50 μ g/ml, 100 μ g/m, establishes the clear water contrast; Repeat 3 times.Adopt spore suspension sprouting method to obtain the EC of each medicament ₅₀Value, and calculate relative coefficient of synergism.

Table 8 oxazole bacterium ketone and Carbatene, Propineb are mixed and make up the Toxicity Determination result to powdery mildew of strawberry

Medicament	Virulence regression equation	Correlation (r)	EC 50. (μg/ml)	EC 5095% confidence interval	Coefficient of synergism (SR)
						Oxazole bacterium ketone (A)	Y＝3.9080+1.6245X	0.9967	5.50	3.5895～9.5327	-
Carbatene (B)	Y＝1.9327+1.5799X	0.9985	102.00	41.9513～ 371.1227	-
						Propineb (C)	Y＝1.8698+1.6500X	0.9977	91.87	37.2827～ 351.8411	-

A+B (1∶1)	Y＝2.9448+1.7847X	0.9990	16.46	9.8096～ 31.7015	0.63
						A+B (1∶2.5)	Y＝2.9858+1.7997X	0.9995	15.27	9.4340～ 27.4264	1.11
A+B (1∶5)	Y＝2.9060+1.7879X	0.9997	17.22	10.4103～ 32.0298	1.51
						A+B (1∶10)	Y＝2.7354+1.8979X	0.9995	18.05	10.8249～ 34.0156	2.18
A+B (1∶20)	Y＝2.6932+1.8123X	0.9996	21.73	12.4743～ 44.2218	2.56
						A+B (1∶40)	Y＝2.7138+1.4798X	0.9988	41.15	20.3312～ 109.4111	1.74
A+B (1∶80)	Y＝2.7997+1.2892X	0.9985	60.30	27.9940～ 176.5112	1.39
						A+C (1∶1)	Y＝3.4924+1.5374X	0.9974	11.21	6.8931～ 20.9013	0.93
A+C (1∶2.5)	Y＝3.3526+1.5719X	0.9903	13.07	6.6407～ 40.0903	1.28
						A+C (1∶5)	Y＝2.9107+1.7876X	0.9974	14.75	10.4590～ 22.9168	1.72
A+C (1∶10)	Y＝2.9370+1.7341X	0.9995	17.99	10.6907～ 34.5987	2.10
						A+C (1∶20)	Y＝2.9805+1.6675X	0.9999	18.95	11.2578～ 36.2743	2.77
A+C (1∶40)	Y＝3.1015+1.2666X	0.9929	37.45	15.0363～ 184.7745	1.77
						A+C (1∶80)	Y＝2.5266+1.4687X	0.9917	56.71	21.5220～ 295.9223	1.36

1-8 can find out that oxazole bacterium ketone and Carbatene or Propineb are composite from table; Weight ratio is 1: 5～1: 20, and germs such as powdery mildew, downy mildew and anthracnose, early blight of tomato, apple marssonina leaf spot and the spot defoliation of greengrocery, melon and fruit class, grape, citrus scab, black spot of fruit tree are all had synergistic effect.

Below in conjunction with embodiment content of the present invention is described further.

FORMULATION EXAMPLE 1:

Take by weighing 5% oxazole bacterium ketone, 55% Carbatene, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 3% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 2% methenamine, 3% diatomite, 5% glucose, kaolin adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 60% oxazole bacterium ketone Carbatene water dispersible granules.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Carbatene can change between 1: 5～1: 20, and both total weight items still are 60%, form new embodiment.

FORMULATION EXAMPLE 2

Take by weighing 6% oxazole bacterium ketone, 64% Carbatene, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 1%K-12 (lauryl sodium sulfate), 2% methenamine, 1% carboxymethyl cellulose, 3% urea, diatomite adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 70% oxazole bacterium ketone Carbatene water dispersible granules.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Carbatene can change between 1: 5～1: 20, and both total weight items still are 70%, form new embodiment.

FORMULATION EXAMPLE 3:

Take by weighing 3.5% oxazole bacterium ketone, 70% Carbatene, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% corn starch, 4% sulphur ammonium, 1% sodium citrate, attapulgite adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 73.5% oxazole bacterium ketone Carbatene water dispersible granules.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Carbatene can change between 1: 5～1: 20, and both total weight items still are 73.5%, form new embodiment.

FORMULATION EXAMPLE 4:

Take by weighing 10% oxazole bacterium ketone, 50% Carbatene, 4%Morwet D-425 (alkyl naphthalene sulfonic acid condensation polymer sodium salt, Akzo Nobel N.V. produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% corn starch, 5% sucrose, 2% methenamine, kaolin adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 60% oxazole bacterium ketone Carbatene water dispersible granules.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Carbatene can change between 1: 5～1: 20, and both total weight items still are 60%, form new embodiment.

FORMULATION EXAMPLE 5

Take by weighing 6% oxazole bacterium ketone, 36% Propineb, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 2% methenamine, 1% carboxymethyl cellulose, 5% urea, diatomite adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 42% oxazole bacterium ketone Propineb water dispersible granules.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Propineb can change between 1: 5～1: 20, and both total weight items still are 42%, form new embodiment.

FORMULATION EXAMPLE 6:

Take by weighing 5% oxazole bacterium ketone, 50% Propineb, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 3% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 2% methenamine, 5% glucose, diatomite soil adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 55% oxazole bacterium ketone Propineb water dispersible granules.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Propineb can change between 1: 5～1: 20, and both total weight items still are 55%, form new embodiment.

FORMULATION EXAMPLE 7:

Take by weighing 3% oxazole bacterium ketone, 60% Propineb, 4%Morwet D-425 (alkyl naphthalene sulfonic acid condensation polymer sodium salt, Akzo Nobel N.V. produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% corn starch, 5% sulphur ammonium, 2% methenamine, fine particle calcium carbonate adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 63% oxazole bacterium ketone Propineb water dispersible granules.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Propineb can change between 1: 5～1: 20, and both total weight items still are 63%, form new embodiment.

FORMULATION EXAMPLE 8:

Take by weighing 4% oxazole bacterium ketone, 60% Propineb, 4%Morwet D-425 (alkyl naphthalene sulfonic acid condensation polymer sodium salt, Akzo Nobel N.V. produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2%K-12 (lauryl sodium sulfate), 3% corn starch, 5% sucrose, 2% methenamine, kaolin adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 64% oxazole bacterium ketone Propineb water dispersible granules.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Propineb can change between 1: 5～1: 20, and both total weight items still are 64%, form new embodiment.

FORMULATION EXAMPLE 9

Take by weighing 5% oxazole bacterium ketone, 65% Carbatene, 5% calcium lignosulfonate, 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 5% white carbon black, 2% methenamine, kaolin and add to 100% weight portion.Above-mentioned raw materials makes 70% oxazole bacterium ketone thioneb wettable powder through mixing behind the comminution by gas stream.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Carbatene can change between 1: 5～1: 20, and both total weight items still are 70%, form new embodiment.

FORMULATION EXAMPLE 10

Take by weighing 8% oxazole bacterium ketone, 64% Propineb, 4% calcium lignosulfonate, 3%TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% white carbon black, 2% sodium citrate, fine particle calcium carbonate adds to 100% weight portion.Above-mentioned raw materials makes 72% oxazole bacterium ketone Wettable propineb powder through mixing behind the comminution by gas stream.

The part by weight of present embodiment Zhong oxazole bacterium ketone and Propineb can change between 1: 5～1: 20, and both total weight items still are 72%, form new embodiment.

FORMULATION EXAMPLE 11

Take by weighing 10% oxazole bacterium ketone, 50% Propineb, 4% calcium lignosulfonate, 4% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 5% white carbon black, 2% methenamine, attapulgite and add to 100% weight portion.Above-mentioned raw materials makes 60% oxazole bacterium ketone Wettable propineb powder through mixing behind the comminution by gas stream.

The part by weight of embodiment Zhong oxazole bacterium ketone and Propineb can change between 1: 5～1: 20, and both total weight items still are 60%, form new embodiment.

FORMULATION EXAMPLE 12

Take by weighing 7% oxazole bacterium ketone, 70% Carbatene, 5% calcium lignosulfonate, 2% Nekal BX (nekal), 1%K-12 (lauryl sodium sulfate), 3% white carbon black, 1% citric acid, kaolin and add to 100% weight portion.Above-mentioned raw materials makes 77% oxazole bacterium ketone thioneb wettable powder through mixing behind the comminution by gas stream.

The part by weight of embodiment Zhong oxazole bacterium ketone and Carbatene can change between 1: 5～1: 20, and both total weight items still are 77%, form new embodiment.

Biological instance 1 control cucumber downy mildew field control effectiveness test

Control cucumber downy mildew field control effectiveness test carries out field control effectiveness test with reference to " field control effectiveness test criterion () GB/T17980.26-2000 bactericidal agent for preventing and treating cucumber downy mildew ".The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 30m ², repeat investigation state of an illness radix before the dispenser, dispenser twice altogether, 7 days investigation prevention effects before each dispenser and after the last dispenser for 4 times.Every sub-district is got 4 investigation at random, looks into two strains at every, and whole sheet leaves are investigated in every strain.Stage division (is unit with the blade):

0 grade: no scab;

1 grade: lesion area accounts for below 5% of whole leaf area;

3 grades: lesion area accounts for the 6%-10% of whole leaf area;

5 grades: lesion area accounts for the 11%-25% of whole leaf area;

7 grades: lesion area accounts for the 26%-50% of whole leaf area;

9 grades: lesion area accounts for more than 50% of whole leaf area.

The drug effect computational methods:

In the formula: CK ₀, CK ₁Be respectively the forward and backward disease index of blank district dispenser;

PT ₀, PT ₁Be respectively the forward and backward disease index of chemicals treatment district dispenser.

Table 9 control cucumber downy mildew field control effectiveness test

After can finding out that from table 9 ， oxazole bacterium ketone and Carbatene or Propineb are mixed according to a certain percentage, have synergistic effect preferably, under certain application dosage, cucumber downy mildew is had better control efficiency, and can significantly reduce the wherein amount of application of each active ingredient.Testing all processing does not all have obvious harmful effect to the growth of cucumber, and leaf look, melon type etc. are all normal, and safety is better.

Biological instance 2 is prevented and treated the powdery mildew of cucumber field control effectiveness test

Prevent and treat powdery mildew of cucumber and adopt district's group arrangement at random, sub-district area 30m with reference to the sub-district processing of " field control effectiveness test criterion () GB/T 17980.30-2000 bactericidal agent for preventing and treating powdery mildew of cucumber " test medicine, contrast medicament and blank ², repeat investigation state of an illness radix before the dispenser, 7 days investigation prevention effects before the next dispenser and after the last dispenser for 4 times.Every sub-district is got 4 investigation at random, looks into two strains at every, and whole sheet leaves are investigated in every strain.

Table 10 is prevented and treated the powdery mildew of cucumber field control effectiveness test

After can finding out that from table 10 ， oxazole bacterium ketone and zineb and Propineb are mixed according to a certain percentage, have synergistic effect preferably, under certain application dosage, powdery mildew of cucumber is had better control efficiency, and can significantly reduce the wherein amount of application of each active ingredient.Testing all processing does not all have obvious harmful effect to the growth of cucumber, and leaf look, melon type etc. are all normal, and safety is better.

Comprehensively above-mentioned, the present invention prevents and treats greengrocery, melon and fruit class, powdery mildew, downy mildew and anthracnose, early blight of tomato, apple marssonina leaf spot and the spot defoliation of grape, citrus scab, black spot of fruit tree, and component is reasonable; Treatment adds protective effect, and bactericidal effect is good, reduces the medication number of times; Drug cost is low; And its activity and bactericidal effect are not the simple superposition of each composition activity, but significant synergistic effect is arranged, and slow down the generation of resistance; Good to crop safety, meet the security requirement of pesticidal preparations.

Claims (5)

1. agricultural bactericidal composition that contains oxazole bacterium ketone is characterized in that comprising:

A) a property one-tenth alive divides oxazole bacterium ketone;

B) second active component Carbatene or the Propineb;

The weight ratio of first active component and second active component is 1:5 ~ 1:20.

2. according to the agricultural bactericidal composition of the said Han oxazole of claim 1 bacterium ketone, it is characterized in that: the cumulant of first active component and second active component is 10%～80% of a said composition total weight.

3. according to the agricultural bactericidal composition of the said Han oxazole of claim 2 bacterium ketone, it is characterized in that: the cumulant of first active component and second active component is 40%～80% of a said composition total weight.

4. according to the agricultural bactericidal composition of the said Han oxazole of claim 1 bacterium ketone, it is characterized in that: said composition is water dispersible granules or wetting powder.

5. the agricultural bactericidal composition of the said Han oxazole of claim 1 bacterium ketone is in control greengrocery, melon and fruit class powdery mildew, downy mildew, anthracnose, early blight of tomato, apple marssonina leaf spot, spot defoliation, citrus scab, the application in the black spot of fruit tree.