CN1016281B - Optical measurement method and device for automatic analyzer - Google Patents
Optical measurement method and device for automatic analyzerInfo
- Publication number
- CN1016281B CN1016281B CN88100938A CN88100938A CN1016281B CN 1016281 B CN1016281 B CN 1016281B CN 88100938 A CN88100938 A CN 88100938A CN 88100938 A CN88100938 A CN 88100938A CN 1016281 B CN1016281 B CN 1016281B
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- reaction tube
- optical detecting
- light
- analysing apparatus
- automatic analysing
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- 230000003287 optical effect Effects 0.000 title claims abstract description 74
- 238000000691 measurement method Methods 0.000 title abstract 2
- 238000006243 chemical reaction Methods 0.000 claims abstract description 92
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 11
- 230000007246 mechanism Effects 0.000 claims abstract description 7
- 238000005259 measurement Methods 0.000 claims abstract description 6
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 27
- 230000005540 biological transmission Effects 0.000 claims description 25
- 239000000376 reactant Substances 0.000 claims description 21
- 238000002835 absorbance Methods 0.000 claims description 16
- 238000000746 purification Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000004458 analytical method Methods 0.000 abstract description 5
- 238000002834 transmittance Methods 0.000 abstract description 3
- 230000001900 immune effect Effects 0.000 abstract 1
- 238000004611 spectroscopical analysis Methods 0.000 abstract 1
- 230000008901 benefit Effects 0.000 description 5
- 230000001447 compensatory effect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000006303 photolysis reaction Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000013098 chemical test method Methods 0.000 description 1
- RKTYLMNFRDHKIL-UHFFFAOYSA-N copper;5,10,15,20-tetraphenylporphyrin-22,24-diide Chemical compound [Cu+2].C1=CC(C(=C2C=CC([N-]2)=C(C=2C=CC=CC=2)C=2C=CC(N=2)=C(C=2C=CC=CC=2)C2=CC=C3[N-]2)C=2C=CC=CC=2)=NC1=C3C1=CC=CC=C1 RKTYLMNFRDHKIL-UHFFFAOYSA-N 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000015843 photosynthesis, light reaction Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
- G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/12—Generating the spectrum; Monochromators
- G01J3/18—Generating the spectrum; Monochromators using diffraction elements, e.g. grating
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N2021/3129—Determining multicomponents by multiwavelength light
- G01N2021/3133—Determining multicomponents by multiwavelength light with selection of wavelengths before the sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0439—Rotary sample carriers, i.e. carousels
- G01N2035/0444—Rotary sample carriers, i.e. carousels for cuvettes or reaction vessels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0415—Carrusel, sequential
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Engineering & Computer Science (AREA)
- Plasma & Fusion (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
使固定所需数目的反应管(1)的反应管支撑架(2)步进转动，在光学测定位置(c)上，对上述各反应管(1)内的样品和试剂的化学反应状态进行光学测定，像这样构成的进行生物化学和免疫学分析的自动分析装置(A)的光学测定法，是通过由转动机构(24)进行往复转动控制的光■(23)把上述测定光(λ)变成测定项目所对应的单一波长光。并用该单一波长光采用所谓“前分光方式”对空气屋和反应液的光透射率进行光学测定的。
The reaction tube support frame (2) holding a required number of reaction tubes (1) is rotated stepwise, and the chemical reaction state of the sample and the reagent in each reaction tube (1) is optically measured at the optical measurement position (c). The optical measurement method of the automatic analysis device (A) for biochemical and immunological analysis constructed in this way is to convert the measurement light (λ) into a single wavelength light corresponding to the measurement item by the light (23) controlled by the reciprocating rotation of the rotation mechanism (24). The light transmittance of the air chamber and the reaction liquid is optically measured by the so-called "front spectroscopic method" using the single wavelength light.
Description
The present invention relates to a kind of optical detecting method and relative assembly that is applicable to the automatic analysing apparatus that carries out biological chemistry and immune analysis, particularly relate to the optical detecting method and the relative assembly of the complete novelty that adopts so-called preceding beam split mode.
As everyone knows, aspect the automatic analysing apparatus that carries out biological chemistry and immune analysis,, optical detecting method that adopts so-called preceding beam split mode and the optical detecting method that adopts back beam split mode are arranged as the mensuration means of reactant liquor.
The optical detecting method of beam split mode before adopting, be reactant liquor to be housed (corresponding to the reagent of the sample and the project of mensuration, be a kind of liquid that is under the chemical reaction state, below identical) reaction tube arrive before the optical detecting position, with a kind of mensuration rayed it, this mensuration only earlier is transformed into single wavelength light corresponding to the project of mensuration, the absorbance of assaying reaction pipe then with optical filter.
So, adopting the problem that exists in the optical detecting method of above-mentioned like that preceding beam split mode in the past is because must be before reaction tube arrives the said determination position, change optical filter corresponding to the said determination project, therefore make the translational speed of reaction tube realize that high speed is very difficult, and the number of packages of per hour handling is less than above-mentioned back beam split mode greatly.
Adopt above-mentioned in the past like that before another problem in the optical detecting method of beam split mode be, the mensuration project is subjected to the restricted number of optical filter, and since the timeliness of above-mentioned optical filter make and measure precision and reduce.
On the other hand, adopt the optical detecting method of above-mentioned back beam split mode, when the reaction tube in the bracing frame of the reaction tube that is fixed in rotation passes through the optical detecting position, arrive the reaction tube of this position with the long mensuration rayed of all-wave, to its transmitted light grating beam splitting, measure the necessary wavelengths absorbance then.
Adopt the advantage of the optical detecting method of this back beam split mode to be, can measure the chemical reaction state of the total overall reaction liquid that is fixed on the reaction tube bracing frame at a high speed, in addition, in the time course that can measure each reactant liquor, can also measure the timeliness that compensates light-source brightness when measuring and with respect to the blank value of the transmitted light of reaction tube with dual wavelength, therefore, the reliability of mensuration precision is very high.
But, adopt the optical detecting method of above-mentioned such in the past back beam split mode, except above-mentioned many advantages are arranged, also has a fatal shortcoming, promptly by the long rayed reactant liquor of all-wave, for example, because in the composition of an anaphylaxis corpse or other object for laboratory examination and chemical testing etc. and reagent, therefore the disperse of measured value between each reactant liquor takes place by measuring the photolysis reactions that wavelength wavelength in addition causes in generation, not high enough to the reliability of measuring precision.
In addition, adopting above-mentionedly in the past like that in the optical detecting method of back beam split mode, existing the amplitude of photosensor of accepting to carry out each wavelength light that the wavelength beam split becomes limited, can not select problem near this wavelength light below amplitude with grating.
And, in the optical detecting method that adopts above-mentioned such in the past back beam split mode, another problem that exists is or not single wavelength because see through the mensuration light of reaction tube, the photocurrent voltage conversion circuit thus need be measure wavelength number (for example, under the situation of measuring 12 wavelength is 12), therefore device is very complicated, and control is difficulty also, and this device price is higher.
The present invention releases in view of this present situation, its purpose is to provide a kind of and has the optical detecting method of carrying out on the automatic analysing apparatus of beam split mode and the back complete novelty of beam split mode advantage separately before so-called, promptly first, as a kind of optical detecting method of on automatic analysing apparatus, carrying out, what adopt is preceding beam split mode, it can not measure the photodecomposition that the wavelength light beyond the wavelength light causes, does not adopt optical filter yet; The second, timeliness that can compensatory light brightness and corresponding to the deviation of the transmitted light of each reaction tube; The 3rd, can simplify optical detecting device significantly, reduce cost, increase substantially the reliability of measuring precision, can be with all wavelengths light METHOD FOR CONTINUOUS DETERMINATION, finding speed is also the same with the method for optically measuring that adopts the sort of in the past back beam split mode.
To achieve these goals, the invention is characterized in, method of optically measuring with automatic analysing apparatus is a prerequisite, the formation of this automatic analysing apparatus is that the reaction tube bracing frame of fixing required number reaction tube is rotated, chemical reaction state to sample and reagent in above-mentioned each reaction tube on the optical detecting position carries out optical detecting, grating by back and forth being rotated control by rotating mechanism becomes single wavelength light corresponding to the project of mensuration to above-mentioned mensuration light, carries out the optical detecting of the light transmission of air layer and reactant liquor with this single wavelength light.
And, the invention is characterized in, automatic analysing apparatus consists of the following components, promptly fixed the reaction tube bracing frame of requisite number purpose reaction tube, the driving mechanism that this reaction tube bracing frame is rotated, mensuration makes the optical detecting device of this reaction tube bracing frame by the reaction tube absorbance of optical detecting position by rotation: in order to realize said method, optical detecting device with automatic analysing apparatus is a prerequisite, before the mensuration light that shines above-mentioned optical detecting device to reaction tube, with the single wavelength light of the grating selection of back and forth rotating control by rotating mechanism corresponding to the project of mensuration, with this selecteed single wavelength rayed air layer and reaction tube, and the light transmission of this air layer and reactant liquor relatively, to carry out the colorimetric optical detecting.
Therefore, in the optical detecting that on relating to automatic analysing apparatus of the present invention, is carried out, adopt so-called before the beam split mode can prevent to occur in the so-called back beam split mode, promptly owing to measuring the photodecomposition that wavelength light wavelength light transmission in addition causes, advantage according to back beam split mode, promptly before measuring chemical state on the optical detecting position, use the light transmission of measuring the reaction tube of dress water purification in advance corresponding to the single wavelength light of measuring project to compensate the deviation of the transmitted light of each reaction tube, the air blank value of simultaneously can be before the optical detecting position crossing the light of air layer with transmission is a benchmark, come the brightness of compensatory light to change, therefore can obtain high precision, the reactant liquor measured value of high reliability.
Also have, in the present invention since adopt so-called before the beam split mode, and the transmission mensuration single wavelength light only of crossing reaction tube, therefore a photocurrent voltage conversion circuit is just much of that, thereby can simplify optical detecting device significantly.
Following simple declaration drawing.
Fig. 1 is the body profile key diagram that automatic analysing apparatus example of the present invention is used in expression, Fig. 2 is the circuit diagram of the signal processing system of the optical detecting device in the expression automatic analysing apparatus, Fig. 3 is the key diagram of expression according to the irradiating state of the single wavelength light of the inventive method selection, Fig. 4 (A) to (C) is the key diagram of representing in turn corresponding to the reaction tube mobile status of above-mentioned single wavelength light, and Fig. 5 is illustrated in the curve map that the reaction tube of the water purification of packing into and transmission each absorbance when crossing air layer is crossed in above-mentioned single wavelength transmittance.
Below, an embodiment of middle expression describes the present invention in detail with reference to the accompanying drawings.
Relevant with present embodiment as shown in Figure 1 automatic analysing apparatus A is made up of following each several part, the reaction tube bracing frame 2 that to be fixed with 40 bottoms be tetragonal tubular reaction tube 1, make this reaction tube bracing frame 2 counterclockwise rotate the impulse motor 3 of 39 container unit towards Fig. 1, on sample dispensing position a, the sampling receptacle 5 interior sample suction pipes 6 that take out the sample of requirement and inject above-mentioned reaction tube 1 from sampling thief 4, on reagent dispensing position b, take out the corresponding required reagent of mensuration project in the reagent container 7 and inject the reagent suction pipe 8 of above-mentioned reaction tube 1, be arranged on the optical detecting device 9 on the c of optical detecting position and discharge reactant liquor after optical detecting finishes and the cleaning device 10 that cleans.
As mentioned above, the structure of reaction tube bracing frame 2 is by impulse motor 3, make the reaction tube 1 of the required sample of on sample dispensing position a dispensing counterclockwise rotate 351 degree (39 container unit) by Fig. 1, the result moves a container to above-mentioned reaction tube 1 by Fig. 1 clockwise direction.Certainly the present invention also is applicable to and makes reaction tube 1 rotate the automatic analysing apparatus of 369 degree (41 container unit) to Fig. 1 clockwise direction.
In addition, at the interval of each reaction tube supported hole 11,11 of above-mentioned reaction tube bracing frame 2, offered the transmittance hole 12 that horizontal direction runs through.Certainly, on above-mentioned each reaction tube supported hole 11, also offered the mensuration optical transmission mensuration hole 14 that horizontal direction is crossed.
The impulse motor 3 that the reaction tube bracing frame 2 of this structure is rotated is driving circuit 15 by control device CPU operation and motor control circuit 16 with certain speed drive control and also by carrying out scrambler 17, the scrambler control circuit 18 of location are controlled rotation direction and angle exactly at a high speed.Can certainly replace above-mentioned impulse motor 3 with servo motor.
Optical detecting device 9 is to be made of light source 20, monochromator 21, photosensor 22 as shown in Figure 1.Above-mentioned monochromator 21 is can be by impulse motor 24 with decided back and forth to rotate synchronously predetermined angular.
Thereby, from above-mentioned light source 20 penetrate the full wavelength light of coming by the lens combination light harvesting after, from the entrance slit 26 directive gratings 23 of monochromator 21.23 handles of above-mentioned grating input to photosensor 22 with the mensuration light that angle was reflected of reflection corresponding to the single wavelength light of the project of mensuration from exit slit 27.
In addition as shown in Figure 2, the single wavelength light that inputs to above-mentioned photosensor 22 particularly is transfused to behind the photocurrent voltage conversion circuit 36, import in the amplifier that amplifies each more than two of one group of wavelength light (being 4 in the present embodiment) that measure wavelength again, until being amplified to assigned voltage, the mensuration magnitude of voltage that is amplified by this amplifier then is through multiplexer 38, from A/D transducer 39, input to LOG transducer 40, carry out required calculation by control device CPU and handle.
Also have, the foregoing description has illustrated the mensuration light that inputs in the photocurrent voltage transformation loop 36, connects each and measures wavelength and be divided into 4 groups situation, but in the present invention, and this grouping is to be divided into more than one group for well.Under the situation that is divided into many groups, can divide into groups according to the size of absorbance.
For example
By amplifier 37a amplify 340～380nm wave band,
By amplifier 37b amplify 410～500nm wave band,
By amplifier 37c amplify 500～600nm wave band,
Amplify 600～750nm wave band by amplifier 37d, selected by multiplexer 38, preferably the angle of resolution is set out.
That is to say,, for example constitute under the situation of output of amplifier 37, when being 10 volts, can controlling to about 0.15 millivolt absorbance variation to every group, and can obtain the high data of reliability with 100% light quantity by 16 A/D transducers 39 by four groups of above-mentioned branches.
Also have, the impulse motor 24 that above-mentioned grating 23 is rotated is driving circuit 28 and the motor control circuits 29 by above-mentioned control device CPU operation, back and forth rotate control with certain speed, and scrambler 30 and the scrambler control circuit 31 of location are controlled its sense of rotation and rotational angle exactly at a high speed by carrying out.Moreover symbol 32 is that accept key, 33 is that CRT, 34 is that memory circuit, 35 is printer among Fig. 1.
With above-mentioned is prerequisite, according to Fig. 3 selection, irradiation operating principle corresponding to the single wavelength light of measuring project is described below.
If 2 rotation times of reaction tube bracing frame are:
T (=T * 10 second
3Microsecond)
The time of passing through of air layer measurement range and reaction tube measurement range and distance are:
T ' second, the d millimeter
Wavelength is switching time: t
1Second
Distance is the P millimeter between the reaction tube 1
The number n of reaction tube 1.
The once rotation of reaction tube bracing frame 2, promptly the take of reaction tube center circle girth P * n(mm) is T second, that is:
t
1=(T * 10
3* d)/(microsecond of P * n)
t
2=(T * 10
3((P)/2-d) microsecond of)/(P * n)
As shown in Figure 5, as at t
2Within the microsecond, can carry out the wavelength switching controls of wavelength switching part with grating 23.
Because required wavelength light is probably between 340～750nm in the biological chemistry automatic analysing apparatus, so available grating 23 is that the wavelength coverage of 410nm is carried out beam split to maximum chromatic dispersion.
For example, under the situation of the concave grating 23 that adopts above-mentioned monochromator 21,
mnλ＝sinα+sinβ
(λ: wavelength (nm), m: number of times, n: the groove number of average 1mm, α: incident angle, β: angle of emergence) calculate from exit slit 27 the α angle and the β angle of the necessary wavelength that takes out with above-mentioned relational expression, control the velocity of rotation of above-mentioned impulse motor 24 with this, and carry out and positioning control with scrambler 29.
In a single day the like above-mentioned mensuration project wavelength light λ that has selected like this with respect to the reaction tube 1 that arrives above-mentioned optical detecting position C
1, then measure project wavelength light λ with this
1The mensuration of carrying out reactant liquor is as following.
At first, shown in Fig. 4 (a),, inject water purification to the reaction tube No3 that reactant liquor should be housed in the final stage of cleaning device 10.At this moment just on the optical detecting position, carry out the optical detecting of reaction tube No2.
The reaction tube No3 of water purification is housed like this, press shown in Fig. 4 (b) like that, to counterclockwise rotating.Before this reaction tube No3 arrived optical position, optical detecting device was measured the optical transmission rate (T of transmitted light beam orifice 12 earlier
3B), then as Fig. 4 (c) shown in like that, the light transmission when assaying reaction pipe No3 passes through above-mentioned optical detecting position C (T '
3B) be benchmark with both light transmission, obtain the reaction tube sky that is about to measure corresponding to reaction tube No3 from absorbance OD from following formula
3, and memory is in control device CPU.
OD
3=log 1/(T
3B) -log 1/(T
3B)
=log (T
3B)/(T
3B)
Then, if on above-mentioned optical detecting position C, with by said determination project wavelength light λ, the reactant liquor behind working sample and first kind of reagent and the second kind of reagent chemical reaction, then its absorbance OD
3oFor
OD
30=log (T′
30)/(T
30)
Thus, measure the blank value absorbance OD ' afterwards of light-source brightness and each reaction tube
3oBe OD
' 3o=OD
3o-OD
3o
If obtain absorbance OD ' like this
3o, so just can carry out absorbance OD to standard substance
Ng' calculation handle, try to achieve final reactant liquor measured value.
The absorbance OD of standard substance
Ng' be to measure with said determination project wavelength light λ, available following formula is obtained, promptly
OD
ng′＝OD
ng-OD
nb
When getting its concentration when being f, OD
3oConcentration f
30For
f
30= (OD
30-OD
38)/(OD
ns-OD
n8) ×f
= (OD
30′)/(OD
ns′) ×f
Obtain the chemical reaction value (reactant liquor measured value) of reactant liquor, after then this reactant liquor measured value input control device CPU being remembered, for example come out with printer prints.
As mentioned above, among the present invention with the reaction tube absorbance of injecting water purification (T '
3B) and air layer absorbance (T
3B) be benchmark, calculate the reasons are as follows of reactant liquor measured value.
In other words, in the automatic analysing apparatus of reaction tube stepping rotation formula, during with single wavelength assaying reaction liquid, measure the optical detecting method of mode with dual wavelength and compare, very difficult to the compensation of light transmission deviation between the timeliness of light-source brightness (Wavelength strength) and the reaction tube.
The present invention is in order to address this problem, with the extinction of reaction tube that water purification is housed bright (T '
3B) be benchmark, the measured value of compensatory reaction liquid is with the deviation of light transmission between this compensatory reaction pipe, by trying to achieve the absorbance (T of air layer
3B) carry out timeliness compensation to light-source brightness (Wavelength strength), thus guarantee to measure the reliability of precision.
Relating to the optical detecting method of carrying out on the automatic analysing apparatus of the present invention, be to adopt above-mentioned version, therefore can prevent to be seen in the so-called back beam split mode by beam split mode before so-called, the photolysis that is produced because of the wavelength light transmission of measuring beyond the wavelength light, and can increase substantially the reliability of measuring precision.
Secondly, the present invention is by adopting the advantage of the sort of in the past back beam split mode, promptly before measuring the locational chemical state of optical detecting,, compensate the transmitted light deviation of each reaction tube by measure the method for the reaction tube light transmission that water purification is housed in advance corresponding to the single wavelength of the project of mensuration.
Moreover because the air blank value that the present invention can cross the light of air layer with transmission before the optical detecting position is a benchmark, the brightness of compensatory light changes, thereby can in automatically analyzing, try to achieve the reactant liquor measured value of high precision high reliability, simultaneously, such in the past automatic analysing apparatus is such, with full wavelength light the situation that all reaction tubes carry out also doing to remember after blank value is measured is compared when device brings into operation.The memory capacity of control device can reduce significantly.For example, when measuring the blank value of 30 by-reaction pipes with 15 kinds of wavelength light, automatic analysing apparatus must be remembered 450 data like that in the past, in contrast in the present invention, as long as a data unit of memory, the memory capacity that therefore can reduce control device reduces cost significantly.
In addition because adopted so-called preceding beam split mode among the present invention, the mensuration that reaction tube is crossed in transmission is single wavelength light only, therefore a photocurrent voltage conversion circuit is just much of that, thereby can simplify optical detecting device etc. significantly, will realize many good effects.
Claims (6)
1, a kind of optical detecting device in automatic analysing apparatus, it is characterized in that, automatic analysing apparatus has following a few part to constitute, the i.e. fixing reaction tube bracing frame of requisite number purpose reaction tube, the rotating mechanism that this reaction tube bracing frame is rotated, make the optical detecting device of this reaction tube bracing frame by rotation through optical detecting position finding reaction tube absorbance, on the optical detecting device of this automatic analysing apparatus, by back and forth rotate the grating of control by rotating mechanism, select single wavelength light before with the mensuration rayed reaction tube of above-mentioned optical detecting device corresponding to the project of mensuration, single wavelength rayed air layer and reaction tube with this selection, the light transmission of this air layer and reactant liquor relatively is so that carry out the colorimetric optical detecting.
2, the optical detecting device in the automatic analysing apparatus described in claim 1, it is characterized in that, to the compensation of light transmission deviation between the compensation of the light-source brightness of the measured value of previous reaction liquid and reaction tube, be that benchmark carries out with air layer light transmission of being measured with above-mentioned single wavelength light before the optical detecting position and the same reaction tube light transmission that water purification is housed.
3, the optical detecting device in the automatic analysing apparatus as claimed in claim 1 or 2, it is characterized in that, the said determination wavelength is divided into several groups, amplifies the mensuration light that inputs to the photocurrent voltage changer and input to multiplexer by the amplifier that sets by each set of wavelengths.
The optical detecting method of 4, on automatic analysing apparatus as claimed in claim 1, carrying out, it is characterized in that, the reaction tube bracing frame of requisite number purpose reaction tube is rotated, chemical reaction state to sample and reagent in above-mentioned each reaction tube on the optical detecting position carries out optical detecting, in the optical detecting method of automatic analysing apparatus with this structure, with the grating that back and forth rotates control by rotating mechanism, said determination light is become corresponding to the single wavelength light of measuring project, the light transmission of air layer and reactant liquor is carried out optical detecting with this single wavelength light.
The optical detecting method of 5, on automatic analysing apparatus as claimed in claim 4, carrying out, it is characterized in that, be that benchmark carry out with the air layer light transmission with above-mentioned single wavelength light measurement before the optical detecting position with the same reaction tube light transmission that is incorporated with water purification to the compensation of light transmission deviation between the compensation of the light-source brightness of the measured value of above-mentioned reactant liquor and the reaction tube.
The optical detecting method of 6, on the automatic analysing apparatus described in claim 4 or 5, carrying out, it is characterized in that, the said determination wavelength is divided into several groups, the mensuration light of using the amplifier that sets by each set of wavelengths to amplify to input to the photocurrent voltage changer, and amplify and input to multiplexer.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP35877/87 | 1987-02-20 | ||
| JP3587787 | 1987-02-20 | ||
| JP62323774A JPS64451A (en) | 1987-02-20 | 1987-12-23 | Method and instrument for optical measurement with automatic analysis device |
| JP323774/87 | 1987-12-23 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN88100938A CN88100938A (en) | 1988-09-14 |
| CN1016281B true CN1016281B (en) | 1992-04-15 |
Family
ID=26374883
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN88100938A Expired CN1016281B (en) | 1987-02-20 | 1988-02-15 | Optical measurement method and device for automatic analyzer |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0303707A4 (en) |
| JP (1) | JPS64451A (en) |
| KR (1) | KR890700820A (en) |
| CN (1) | CN1016281B (en) |
| AU (1) | AU605840B2 (en) |
| FI (1) | FI884609A7 (en) |
| WO (1) | WO1988006286A1 (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01145552A (en) * | 1987-12-02 | 1989-06-07 | Olympus Optical Co Ltd | Automatic apparatus for analysis |
| US5116122A (en) * | 1991-03-14 | 1992-05-26 | Shimadzu Corporation | Spectrophotometer |
| US5345395A (en) * | 1991-10-31 | 1994-09-06 | Baxter Diagnostics Inc. | Specimen processing and analyzing systems and methods using photometry |
| AUPN825796A0 (en) * | 1996-02-26 | 1996-03-14 | Ashdown, Martin | The application of infrared (ir) spectrometry to the investigations of components of blood and other body fluids |
| JP2006125953A (en) * | 2004-10-28 | 2006-05-18 | Jeol Ltd | Biochemical automatic analyzer |
| JP4598682B2 (en) | 2006-01-23 | 2010-12-15 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP5134862B2 (en) * | 2007-05-16 | 2013-01-30 | 株式会社日立ハイテクノロジーズ | Analysis equipment |
| JP5743558B2 (en) * | 2011-01-12 | 2015-07-01 | 株式会社東芝 | Analysis equipment |
| JP6129568B2 (en) * | 2013-01-31 | 2017-05-17 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP6276087B2 (en) * | 2014-03-28 | 2018-02-07 | シスメックス株式会社 | Sample analyzer and sample analysis method |
| JP6312313B2 (en) * | 2014-04-17 | 2018-04-18 | 日本電子株式会社 | Automatic analyzer and automatic analysis method |
| JP6745397B2 (en) * | 2016-07-21 | 2020-08-26 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッドＳｉｅｍｅｎｓ Ｈｅａｌｔｈｃａｒｅ Ｄｉａｇｎｏｓｔｉｃｓ Ｉｎｃ． | Eliminating intensity drift effects in power ramps using reference measurements between cuvettes |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1648841A1 (en) * | 1967-10-18 | 1971-07-22 | Bodenseewerk Perkin Elmer Co | Single-beam photometer |
| US3609047A (en) * | 1970-03-02 | 1971-09-28 | Xerox Corp | Single beam photometer system wherein the absorbance of a sample is determined relative to a reference |
| DE2408646A1 (en) * | 1974-02-22 | 1975-08-28 | Max Planck Gesellschaft | REACTION KINETIC MEASURING DEVICE |
| JPS5630650A (en) * | 1979-08-22 | 1981-03-27 | Hitachi Ltd | Automatic chemical analyzer |
| FR2470962A1 (en) * | 1979-11-30 | 1981-06-12 | Instruments Sa | Optical measurement device for liq. sample concentration - has photocells mounted on mobile support and surrounding sample, in path of light source and opaque screen |
| JPS5772047A (en) * | 1980-10-24 | 1982-05-06 | Olympus Optical Co Ltd | Component analyzing method |
| JPS60142234A (en) * | 1983-12-28 | 1985-07-27 | Shimadzu Corp | Automatic analysis device |
| JPS6166950A (en) * | 1984-09-11 | 1986-04-05 | Nippon Tectron Co Ltd | Data processing unit for automatic blood analysis instrument |
| JPH0518090A (en) * | 1991-07-10 | 1993-01-26 | Asahi Chem Ind Co Ltd | Thin stone-composite panel structure |
| JP2957797B2 (en) * | 1992-03-18 | 1999-10-06 | 三洋電機株式会社 | Multiple recording method of optical recording medium |
-
1987
- 1987-12-23 JP JP62323774A patent/JPS64451A/en active Pending
-
1988
- 1988-02-15 CN CN88100938A patent/CN1016281B/en not_active Expired
- 1988-02-17 EP EP19880901914 patent/EP0303707A4/en not_active Withdrawn
- 1988-02-17 WO PCT/JP1988/000161 patent/WO1988006286A1/en not_active Application Discontinuation
- 1988-02-17 AU AU12977/88A patent/AU605840B2/en not_active Ceased
- 1988-09-12 KR KR1019880701102A patent/KR890700820A/en not_active Ceased
- 1988-10-07 FI FI884609A patent/FI884609A7/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| WO1988006286A1 (en) | 1988-08-25 |
| EP0303707A4 (en) | 1990-05-14 |
| JPS64451A (en) | 1989-01-05 |
| KR890700820A (en) | 1989-04-27 |
| FI884609A0 (en) | 1988-10-07 |
| AU1297788A (en) | 1988-09-14 |
| EP0303707A1 (en) | 1989-02-22 |
| AU605840B2 (en) | 1991-01-24 |
| FI884609A7 (en) | 1988-10-07 |
| CN88100938A (en) | 1988-09-14 |
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