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CN101564472A - Method for producing linaloe by aquilaria sinensis trees - Google Patents

Method for producing linaloe by aquilaria sinensis trees Download PDF

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Publication number
CN101564472A
CN101564472A CNA2009100850823A CN200910085082A CN101564472A CN 101564472 A CN101564472 A CN 101564472A CN A2009100850823 A CNA2009100850823 A CN A2009100850823A CN 200910085082 A CN200910085082 A CN 200910085082A CN 101564472 A CN101564472 A CN 101564472A
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CN
China
Prior art keywords
lignum aquilariae
aquilariae resinatum
linaloe
aquilaria sinensis
trunk
Prior art date
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Pending
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CNA2009100850823A
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Chinese (zh)
Inventor
魏建和
张争
杨云
孟慧
冯锦东
隋春
陈伟平
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Institute of Medicinal Plant Development of CAMS and PUMC
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Institute of Medicinal Plant Development of CAMS and PUMC
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Priority to CNA2009100850823A priority Critical patent/CN101564472A/en
Publication of CN101564472A publication Critical patent/CN101564472A/en
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Abstract

The invention discloses a method for using an actualcautery to induce to produce linaloe on aquilaria sinensis trees artificially cultivated or wild aquilaria sinensis trees. The method comprises the following steps of: using red-hot iron drill bit to drill on the aquilaria sinensis trunk with more than five years, burning and scalding plant cells around the drill hole, activating defensive reaction of the aquilaria sinensis trunk, inducing to produce medicinal secondary metabolites, namely linaloe; after 3 to 12 months and the trunk is inosculated with the linaloe, cutting brownish-black eaglewood formed near the drilling hole, drying, crushing, then using alcohol to extract and obtaining the linaloe oil. The method for using the actualcautery to induce aquilaria sinensis to produce linaloe has simple technique, convenient operation and obvious inducing effect, can greatly shorten the production period of the linaloe, is applicable to large-scale standardization and commercialization production, provides a new effective path for protecting, developing and utilizing the aquilaria sinensis tree resources and has important economical, social and ecological benefits.

Description

Produce the method for Lignum Aquilariae Resinatum by Rhizoma Atractylodis macrocephalae's XIANGSHU
Technical field
The present invention relates to a kind of fire that on artificial culture Rhizoma Atractylodis macrocephalae XIANGSHU or wild Rhizoma Atractylodis macrocephalae's XIANGSHU, utilizes and iron the method for inducing the production Lignum Aquilariae Resinatum of sending out.
Background technology
Lignum Aquilariae Resinatum Aquilaria sinensis (Lour.) Gilg claims TUCHENXIANG again, belongs to the thymelaeceae eaglewood, is the distinctive torrid zone of China and subtropical evergreen arbor, is unique plant origin that China produces famous and precious aromatics medical material Lignum Aquilariae Resinatum.The history that Lignum Aquilariae Resinatum is used as medicine and had in China's Chinese medicine, Tibetanmedicine and Ayurvedic medicine several thousand.Hong Kong also abounds with in history and exports Lignum Aquilariae Resinatum because of it gains the name.Because high value of Lignum Aquilariae Resinatum (the international knock-down price of present superfine LIGNUM AQUILARIAE RESINATUM MOSS is up to 10000 dollars/kilogram) and international market demand are very big, Lignum Aquilariae Resinatum is robbed the formula overcut for a long time, and its primary forest destroys totally substantially.Lignum Aquilariae Resinatum in 1999 is listed in national secondary endangered protective plant and records in " Chinese Plants Red Data Book ".Lignum Aquilariae Resinatum was listed in " the red register of the compromised plant of World Conservation Union " by World Conservation Union (IUCN) in 2000.Lignum Aquilariae Resinatum had been listed " Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) " (ITES, i.e. Washington pact) appendix in 2004, and the Lignum Aquilariae Resinatum that originates in various countries' production Lignum Aquilariae Resinatum such as Southeast Asia simultaneously belongs to all plants and all listed the pact register in.Be protection and the rare Lignum Aquilariae Resinatum resource in imminent danger of sustainable use, some scale Lignum Aquilariae Resinatum artificial growth bases have been built up in Hainan, Guangdong.Therefore, the Lignum Aquilariae Resinatum Edgeworthia chrysantha Lindl. that how fully to develop artificial growth becomes key issue in the current production to alleviate the Lignum Aquilariae Resinatum imbalance between supply and demand.The Lignum Aquilariae Resinatum of self-sow can not Edgeworthia chrysantha Lindl., have only the Lignum Aquilariae Resinatum trunk to be injured or fungal infection after just produce Lignum Aquilariae Resinatum.The Lignum Aquilariae Resinatum of artificial growth must apply artificial processing could Edgeworthia chrysantha Lindl..For quickening and improving Lignum Aquilariae Resinatum Edgeworthia chrysantha Lindl. amount, just carried out mechanical wounding, chemistry injury and the research of fungal infection acceleration Lignum Aquilariae Resinatum Edgeworthia chrysantha Lindl. both at home and abroad the seventies from eighties of last century, but Edgeworthia chrysantha Lindl. efficient is not high and unstable.
Summary of the invention
For solving problem and the defective that exists in above-mentioned, the invention provides a kind of by adopting fiery cauterization to induce to make the method for Lignum Aquilariae Resinatum tree to produce agilawood.
The present invention is achieved by the following technical solutions:
A kind of method by Rhizoma Atractylodis macrocephalae's XIANGSHU production Lignum Aquilariae Resinatum involved in the present invention comprises:
Ironing drill bit by fire holes to the Lignum Aquilariae Resinatum trunk;
Extract near the brownish black Lignum Aquilariae Resinatum that forms the boring;
Remove not resiniferous woody part in the brownish black Lignum Aquilariae Resinatum, become Lignum Aquilariae Resinatum.
The time that extracts between the Lignum Aquilariae Resinatum that is bored into that described white XIANGSHU is done was at least more than 3 months.
Described height to Lignum Aquilariae Resinatum tree boring is at least 5cm apart from ground, and upwards bores a row every 5cm at least successively, and the distance between every gang drill hole is at least 2cm.
Boring on the described Lignum Aquilariae Resinatum trunk comprises 10 rows at least, and every gang drill has 2-3 hole.
The temperature that described fire to the boring of Lignum Aquilariae Resinatum trunk irons drill bit is 600-800 degree centigrade.
The beneficial effect of technical scheme provided by the invention is:
Greatly shorten the cycle that Lignum Aquilariae Resinatum produces, be useful for the large-scale standardized commercialization and produce, protection, the development and utilization of Lignum Aquilariae Resinatum being set resource provide valid approach, have important economy, society and ecological benefits.
Description of drawings
Fig. 1 is a method flow diagram of the present invention;
Fig. 2 is a drill bit sketch map used in the present invention;
Fig. 3 is the sketch map that the inventive method is handled the Lignum Aquilariae Resinatum trunk.
The specific embodiment
For making the purpose, technical solutions and advantages of the present invention clearer, embodiment of the present invention is described further in detail below in conjunction with accompanying drawing:
Present embodiment provides a kind of method by Rhizoma Atractylodis macrocephalae's XIANGSHU production Lignum Aquilariae Resinatum, wherein,
Referring to Fig. 1, this method realizes by utilize fiery cauterization on artificial culture Rhizoma Atractylodis macrocephalae XIANGSHU or wild Rhizoma Atractylodis macrocephalae's XIANGSHU, may further comprise the steps:
Step 101 irons drill bit by fire the Lignum Aquilariae Resinatum trunk is holed;
With special drill bit with tip (referring to Fig. 2, Ф=1.5cm, L=15cm) be heated to 600-800 degree centigrade, present dark red to orange red, to be heated to pyritous drill bit then and wedge the Lignum Aquilariae Resinatum trunk rapidly, take out after cooling, and repeat wedging and take out 1-2 time, the boring of described wedging Lignum Aquilariae Resinatum trunk and the distance 4 on ground are at least 5cm, and it is preferably 10cm; And upwards bore a row 3 every 5cm at least successively, be at least 2cm, be preferably 2cm with 2 distance between the gang drill hole; And boring includes 10 rows (referring to Fig. 3) at least.It is vertical with the stem stalk to hole, and aperture, hole 1 is 1.5cm, and the degree of depth is 5-8cm.
Step 102 extracts near the brownish black Lignum Aquilariae Resinatum that forms the boring, and removes not resiniferous woody part.
Lignum Aquilariae Resinatum trunk boring self-sow after at least 6 months or after 1 year, is adopted the perusal hole thickness of rufous Lignum Aquilariae Resinatum layer on every side.Perhaps make the section of slipping, adopt the form size and the distributed quantity of yellowish-brown resin (being Lignum Aquilariae Resinatum) in observation by light microscope statistics ray cell and the vessel cell, tentatively conclude Lignum Aquilariae Resinatum Edgeworthia chrysantha Lindl. effect; Extract near the brownish black Lignum Aquilariae Resinatum that forms the boring then.
Step 103 is removed not resiniferous woody part with cutter and it is dried, and becomes Lignum Aquilariae Resinatum.
Produce linaloe oil with reference to the hot dipping under the contained ethanol-soluble extractives algoscopy of the Pharmacopoeia of the People's Republic of China item in 2005, and the alcohol extract content of above-mentioned Lignum Aquilariae Resinatum sample is measured.Precision takes by weighing Lignum Aquilariae Resinatum medicinal powder 2.0g, puts in the conical flask of 250ml, the accurate dehydrated alcohol 50ml that adds, jam-pack claims decide weight, leave standstill 1 hour after, the connection reflux condensing tube is heated to boiling, and keeps little and boiled 1 hour.Put coldly, take off round-bottomed flask, jam-pack claims decide weight, and water is supplied the weight that subtracts mistake, shakes up, and with dry filter filtration, is linaloe oil.Precision is measured filtrate 25ml, puts in the evaporating dish that is dried to constant weight, behind evaporate to dryness in the water-bath, in 105 ℃ of dryings 3 hours, moves in the exsiccator, cools off 30 minutes, accurately rapidly claims decide weight, with the content (%) of ethanol-soluble extractives in the dry product calculating medical material.5 parts of same batch sample parallel tests.
Present embodiment scalds extremely, scalds boring plant cell on every side by using red Rhizoma Atractylodis macrocephalae's XIANGSHU bar drill hole of anvil head more than life in 5 years of burning, activates the Lignum Aquilariae Resinatum defense reaction, induces the medicinal secondary metabolites-Lignum Aquilariae Resinatum of generation.
The above; only for the preferable specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claim.

Claims (5)

1, produce the method for Lignum Aquilariae Resinatum by Rhizoma Atractylodis macrocephalae's XIANGSHU, it is characterized in that this method comprises:
Ironing drill bit by fire holes to the Lignum Aquilariae Resinatum trunk;
Extract near the brownish black Lignum Aquilariae Resinatum that forms the boring;
Remove not resiniferous woody part in the brownish black Lignum Aquilariae Resinatum, become Lignum Aquilariae Resinatum.
2, the method by Rhizoma Atractylodis macrocephalae's XIANGSHU production Lignum Aquilariae Resinatum according to claim 1 is characterized in that the time that extracts between the Lignum Aquilariae Resinatum that is bored into that described white XIANGSHU is done was at least more than 3 months.
3, according to claim 1ly produce the method for Lignum Aquilariae Resinatum, it is characterized in that described height to the boring of Lignum Aquilariae Resinatum tree is at least 5cm apart from ground, and upwards bore a row successively, and the distance between every gang drill hole is at least 2cm every 5cm at least by Rhizoma Atractylodis macrocephalae's XIANGSHU.
4, according to claim 1 or 3 described methods, it is characterized in that the boring on the described Lignum Aquilariae Resinatum trunk comprises 10 rows at least, and every gang drill has 2-3 hole by Rhizoma Atractylodis macrocephalae's XIANGSHU production Lignum Aquilariae Resinatum.
5, the method by Rhizoma Atractylodis macrocephalae's XIANGSHU production Lignum Aquilariae Resinatum according to claim 1 is characterized in that, the temperature that described fire to the boring of Lignum Aquilariae Resinatum trunk irons drill bit is 600-800 degree centigrade.
CNA2009100850823A 2009-06-01 2009-06-01 Method for producing linaloe by aquilaria sinensis trees Pending CN101564472A (en)

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101731282B (en) * 2009-12-02 2012-03-21 中国医学科学院药用植物研究所 Tambac inducer and preparation method thereof
CN102499264A (en) * 2011-10-31 2012-06-20 黄汉 Agarwood promoter and method for producing agarwood in Aquilaria sinensis
CN102599015A (en) * 2012-03-22 2012-07-25 蓝均炽 Production method of plate-type Chinese eaglewood
CN102812858A (en) * 2012-07-18 2012-12-12 中山市沉香黄农林发展有限公司 Method for promoting formation of heartwood of aquilaria sinensis tree
CN103039283A (en) * 2013-01-08 2013-04-17 东莞香市莞香沉香研发有限公司 Method for producing agilawood by burning aquilaria trees
CN103063773A (en) * 2012-12-26 2013-04-24 广东省微生物研究所 Detection method for generation of aquilaria resin in aquilaria sinensis
CN103181304A (en) * 2013-01-14 2013-07-03 中山市国林沉香科学研究所 In vivo agilawood formation method for Aquilaria plants
CN108307849A (en) * 2017-01-17 2018-07-24 昱达国际生化科技有限公司 Promote the method for agallochum secretion agalloch eaglewood resin
CN110250211A (en) * 2019-07-08 2019-09-20 中国林业科学研究院热带林业研究所 It is a kind of induce buta-buta Edgeworthia chrysantha bio-physical method and its application
CN110427048A (en) * 2019-08-20 2019-11-08 昆明科晔生物技术有限公司 A kind of new chemical agalloch eaglewood expedites the emergence of device
CN110447416A (en) * 2019-09-12 2019-11-15 文昌琼香神韵沉香专业合作社 A kind of minimally invasive biology of whitewood's incense wood is stale-proof the research method of Edgeworthia chrysantha
CN110692425A (en) * 2019-11-16 2020-01-17 海南迪葳生物科技有限公司 Method for artificially promoting aquilaria sinensis trees to generate agilawood
CN111990102A (en) * 2019-05-27 2020-11-27 东莞市象力液压元件制造有限公司 Environmental protection agalloch eaglewood punches and uses integrative automation tool of hydraulic pressure electromechanics
CN113273427A (en) * 2021-06-07 2021-08-20 国森天香(海南)科技有限公司 Agilawood rotating and forming method
CN113383667A (en) * 2021-07-12 2021-09-14 中国林业科学研究院热带林业研究所 Sal-added aquilaria wood edgeworthia chrysantha method and application thereof

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101731282B (en) * 2009-12-02 2012-03-21 中国医学科学院药用植物研究所 Tambac inducer and preparation method thereof
CN102499264A (en) * 2011-10-31 2012-06-20 黄汉 Agarwood promoter and method for producing agarwood in Aquilaria sinensis
CN102499264B (en) * 2011-10-31 2013-11-06 黄汉 Agarwood promoter and method for producing agarwood in Aquilaria sinensis
CN102599015A (en) * 2012-03-22 2012-07-25 蓝均炽 Production method of plate-type Chinese eaglewood
CN102812858A (en) * 2012-07-18 2012-12-12 中山市沉香黄农林发展有限公司 Method for promoting formation of heartwood of aquilaria sinensis tree
CN103063773A (en) * 2012-12-26 2013-04-24 广东省微生物研究所 Detection method for generation of aquilaria resin in aquilaria sinensis
CN103039283A (en) * 2013-01-08 2013-04-17 东莞香市莞香沉香研发有限公司 Method for producing agilawood by burning aquilaria trees
CN103181304A (en) * 2013-01-14 2013-07-03 中山市国林沉香科学研究所 In vivo agilawood formation method for Aquilaria plants
CN108307849A (en) * 2017-01-17 2018-07-24 昱达国际生化科技有限公司 Promote the method for agallochum secretion agalloch eaglewood resin
CN111990102A (en) * 2019-05-27 2020-11-27 东莞市象力液压元件制造有限公司 Environmental protection agalloch eaglewood punches and uses integrative automation tool of hydraulic pressure electromechanics
CN110250211A (en) * 2019-07-08 2019-09-20 中国林业科学研究院热带林业研究所 It is a kind of induce buta-buta Edgeworthia chrysantha bio-physical method and its application
CN110250211B (en) * 2019-07-08 2021-08-31 中国林业科学研究院热带林业研究所 Biophysical method for inducing aquilaria sinensis to knot fragrance and application thereof
CN110427048A (en) * 2019-08-20 2019-11-08 昆明科晔生物技术有限公司 A kind of new chemical agalloch eaglewood expedites the emergence of device
CN110427048B (en) * 2019-08-20 2022-06-14 山东太樾生物技术有限公司 Novel chemical agilawood growth promoting device
CN110447416A (en) * 2019-09-12 2019-11-15 文昌琼香神韵沉香专业合作社 A kind of minimally invasive biology of whitewood's incense wood is stale-proof the research method of Edgeworthia chrysantha
CN110692425A (en) * 2019-11-16 2020-01-17 海南迪葳生物科技有限公司 Method for artificially promoting aquilaria sinensis trees to generate agilawood
CN113273427A (en) * 2021-06-07 2021-08-20 国森天香(海南)科技有限公司 Agilawood rotating and forming method
CN113383667A (en) * 2021-07-12 2021-09-14 中国林业科学研究院热带林业研究所 Sal-added aquilaria wood edgeworthia chrysantha method and application thereof

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Open date: 20091028