CN101537059B - Anti-enteritis canine parvovirus Chinese medicinal composition and preparation method and applications thereof - Google Patents
Anti-enteritis canine parvovirus Chinese medicinal composition and preparation method and applications thereof Download PDFInfo
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Abstract
The invention discloses an anti-enteritis canine parvovirus Chinese medicinal composition and a preparation method and applications thereof. The Chinese medicinal composition is prepared by the raw materials according to the following weight ratios: 20-150 portions of radix scutellariae, 20-40 portions of radix sophorae falvescentis, 60-100 portions of Chinese pulsatilla root, 25-50 portions of cape jasmine fruit, 20-150 portions of honeysuckle flower, 15-100 portions of bighead atractylodes rhizome, 50-200 portions of cablin potchouli herb and 1-10 portions of astragalus polyose. The preparation method of the Chinese medicinal composition comprises three steps of: extraction of essential oil of the cablin potchouli herb; preparation of the radix scutellariae, the radix sophorae falvescentis, the Chinese pulsatilla root, the cape jasmine fruit, the honeysuckle flower, the bighead atractylodes rhizome and water extract of fcablin potchouli herb residue after the extracting of essential oil; and preparation of the Chinese medicinal composition by mixing the water extract, the astragalus polyose and the essential oil of the cablin potchouli herb totally needs three steps. The Chinese medicinal composition has the efficacies of clearing away heat and toxic materials, cooling blood and stopping dysentery and vomiting, can be prepared into anti-enteritis canine parvovirus drugs, and has the advantages of being high-effective, safe and economical, and the like, thus having good application prospect.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, particularly a kind of Chinese medicine composition of anti-enteritis canine parvovirus also relates to the preparation method and the application of this Chinese medicine composition.
Background technology
Canine parvovirus disease be by Canine Parvovirus (Canine parvovirus, a kind of acute infectious disease that CPV) causes, the sickness rate height, infectiousness is strong, the mortality rate height is that one of the most serious dog infectious disease of dog industry is supported by harm China, can cause great economic loss.This disease is many clinically to be principal character with hemorrhagic enteritis or apyetous myocarditis, so be divided into two kinds on enteritis type and myocarditis type, sees with enteritis type more.
At present, canine parvovirus disease does not still have specific treatment medicine and method.Doctor trained in Western medicine mainly adopts immunotherapy (vaccination, hyper-immune serum etc.), unite symptomatic therapy (giving Bendectin, diarrhea, antipyretic and anti-inflammatory medicine etc.), fluid replacement therapy (replenishing normal saline, glucose solution etc.) and control secondary infection (giving antibiotic etc.) etc. simultaneously and carry out Comprehensive Treatment, but because the genomic variability of Canine Parvovirus is very strong, the immunotherapy effect is undesirable, other householder method also can only be taken stopgap measures, can not effect a permanent cure, and side effect is bigger, easily cause drug resistance, cure rate is lower.
Summary of the invention
In view of this, one of purpose of the present invention is to provide a kind of Chinese medicine composition of anti-enteritis canine parvovirus, the compatibility uniqueness, raw material sources are extensive, with low cost, effect with heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, preventing or arresting vomiting can the high-efficiency prevention and control enteritis canine parvovirus, and toxic and side effects is little.
For reaching this purpose, in a first aspect of the present invention, a kind of Chinese medicine composition of anti-enteritis canine parvovirus is provided, and it is the medicament of being made by the following weight proportion raw material: 20～150 parts of Radix Scutellariaes, 20～40 parts of Radix Sophorae Flavescentiss, 60～100 parts of the Radix Pulsatillaes, 25～50 parts of Fructus Gardeniaes, 20～150 parts of Flos Loniceraes, 15～100 parts of the Rhizoma Atractylodis Macrocephalaes, 50～200 parts of Herba Pogostemonis, 1～10 part of astragalus polysaccharides.
Further, the weight proportion of described each raw material is: 60～120 parts of Radix Scutellariaes, 25～35 parts of Radix Sophorae Flavescentiss, 70～90 parts of the Radix Pulsatillaes, 30～45 parts of Fructus Gardeniaes, 60～120 parts of Flos Loniceraes, 50～90 parts of the Rhizoma Atractylodis Macrocephalaes, 60～150 parts of Herba Pogostemonis, 2～6 parts of astragalus polysaccharidess.
Further, the weight proportion of described each raw material is: 100 parts of Radix Scutellariaes, 30 parts of Radix Sophorae Flavescentiss, 80 parts of the Radix Pulsatillaes, 40 parts of Fructus Gardeniaes, 100 parts of Flos Loniceraes, 80 parts of the Rhizoma Atractylodis Macrocephalaes, 70 parts of Herba Pogostemonis, 2 parts of astragalus polysaccharidess;
Further, described medicament is a granule.
Two of purpose of the present invention is to provide the preparation method of the Chinese medicine composition of described anti-enteritis canine parvovirus, and easy to operation, production cost is low.
For reaching this purpose, in a second aspect of the present invention, provide the preparation method of the Chinese medicine composition of described anti-enteritis canine parvovirus, may further comprise the steps:
A, get Herba Pogostemonis, extract volatile oil by proportioning, the residue after collecting volatile oil respectively and extracting volatile oil, standby;
B, get Herba Pogostemonis residue after Radix Scutellariae, Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Gardeniae, Flos Lonicerae, the Rhizoma Atractylodis Macrocephalae and step a extract volatile oil by proportioning, extracting in water filters, and filtrate concentrates, and makes the water extract, and is standby;
C, get step b gained water extract, add astragalus polysaccharides by proportioning, mix homogeneously is concentrated into the thick paste shape, and cooling adds step a gained Herba Pogostemonis Volatile oil, and mix homogeneously adds acceptable accessories again, makes medicament according to a conventional method.
Further, adopt steam distillation to extract volatile oil among the step a: to add the water logging that is equivalent to 10～15 times of raw material weights and steep after 1～3 hour, extract volatile oil in 70～90 ℃ of distilling under reduced pressure of temperature;
Further, adopt decocting method to prepare the water extract among the step b: decoct with water altogether and extract three times, adding for the first time is equivalent to the water of 5～10 times of raw material weights, soaks to decoct 1～3 hour after 10～14 hours, filters, and collects filtrate; Each adds the water that is equivalent to 2～3 times of raw material weights for the second time and for the third time, decocts 0.5～1.5 hour, filters, and collects filtrate; Merge three times filtrate, concentrate, promptly make the water extract;
Further, medicament described in the step c is a granule;
Further, acceptable accessories described in the step c is dextrin and sucrose.
Three of purpose of the present invention is to provide the application of the Chinese medicine composition of described anti-enteritis canine parvovirus.
For reaching this purpose,, provide the application of Chinese medicine composition in preparation anti-enteritis canine parvovirus medicine of described anti-enteritis canine parvovirus in a third aspect of the present invention.
Beneficial effect of the present invention is: the invention provides Chinese medicine composition of a kind of anti-enteritis canine parvovirus and its production and application, this Chinese medicine composition is raw material with the natural Chinese medicinal herb, the compatibility uniqueness, raw material sources are extensive, preparation method is simple, low production cost, effect with heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, preventing or arresting vomiting, can make the anti-enteritis canine parvovirus medicine, have efficient, safety, economic dispatch advantage, good prospects for application is arranged in the prevention and the treatment field of enteritis canine parvovirus.
The specific embodiment
From the traditional Chinese veterinary medicine theory analysis, the etiology and pathogenesis of enteritis canine parvovirus is the heresy of diseases caused by exogenous pathogenic factor epidemic disease, and heat is moved nutrient blood from the intestinal network, damp and hot gathering in middle Jiao, pass in the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels, and make the gastrointestinal transfer function not normal, cause adverse rising of stomach-QI to be felt sick, damp invasion of lower energizer becomes to rush down; Its control is advisable with heat-clearing and toxic substances removing, the spleen strengthening and damp drying, hemostasis removing heat from blood, stopping nausea and vomiting by lowering the adverse flow of QI.The inventor is according to above-mentioned control principle and traditional Chinese veterinary medicine prescription rule, Radix Scutellariae, Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Gardeniae, Flos Lonicerae, the Rhizoma Atractylodis Macrocephalae, Herba Pogostemonis and the astragalus polysaccharides that selection has heat-clearing and toxic substances removing, dampness removing, dysentery relieving, a preventing or arresting vomiting effect be totally eight flavor medicine reasonable formula: Radix Scutellariae heat clearing and damp drying, eliminating fire and detoxication, hemostasis, Radix Sophorae Flavescentis heat clearing and damp drying, parasite killing, diuresis, Radix Pulsatillae heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery are monarch drug; Fructus Gardeniae pathogenic fire purging relieving restlessness, clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body, Flos Lonicerae heat-clearing and toxic substances removing, wind-heat dissipating are ministerial drug; Herba Pogostemonis eliminating turbid pathogen with aromatics, the preventing or arresting vomiting that whets the appetite, deliver expelling summer-heat, the astragalus polysaccharides raise immunity is an adjuvant drug; Rhizoma Atractylodis Macrocephalae invigorating the spleen and benefiting QI, dampness diuretic are messenger drug; Eight medicines are played the merit of heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, preventing or arresting vomiting altogether, can reach the purpose of effective control enteritis canine parvovirus, have advantages such as good effect, side effect be little.
Each raw material of Chinese medicine composition of the present invention has following character and effect:
Radix Scutellariae is the dry root of labiate Radix Scutellariae Scutellaria baicalensis Georgi; Bitter in the mouth, cold in nature; Return lung, gallbladder, spleen, large intestine, small intestine meridian; The function heat clearing and damp drying, eliminating fire and detoxication, hemostasis, antiabortive; Cure mainly hygropyrexia, fever disease in summer vomiting and nausea uncomfortable in chest, damp and hot feeling of fullness, dysentery, jaundice, the cough due to lung-heat, the hyperpyrexia excessive thirst, heat in blood is told nosebleed, carbuncle sore tumefacting virus, frequent fetal movement.
Radix Sophorae Flavescentis is the dry root of leguminous plant Radix Sophorae Flavescentis Sophora flavescens Ait.; Bitter in the mouth, cold in nature; GUIXIN, liver, stomach, large intestine, urinary bladder channel; The function heat clearing and damp drying, parasite killing, diuresis; Cure mainly hematodiarrhoea, have blood in stool, jaundice urine retention, leucorrhea with red and white discharge, swelling of the vulva pudendal pruritus, eczema, eczema, skin pruritus, scabies leprosy.
The Radix Pulsatillae is the dry root of ranunculaceae plant Radix Pulsatillae Pulsatilla chinensis (Bge.) Regel; Bitter in the mouth, cold in nature; Return stomach, large intestine channel; The function heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery; Cure mainly toxic-heat and blood stasis, pudendal pruritus leukorrhagia, amebic dysentery.
Fructus Gardeniae is the dry mature fruit of Maguireothamnus speciosus Fructus Gardeniae Gardenia jasminoides Ellis; Bitter in the mouth, cold in nature; GUIXIN, lung, tri-jiao channel; Function pathogenic fire purging relieving restlessness, clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body; It is vexed to cure mainly calentura, the jaundice dark coloured urine, and blood strangury and dry pain, heat in blood is told nosebleed, conjunctival congestion and swelling pain, pathogenic fire,toxin and furuncles.
Flos Lonicerae is the dry flower of caprifoliaceae plant Radix Ophiopogonis Lonicera japonica Thund. or the flower that band is just opened; Sweet in the mouth, cold in nature; Return lung, the heart, stomach warp; The function heat-clearing and toxic substances removing, wind-heat dissipating; Cure mainly the carbuncle furuncle, sore throat, erysipelas, toxic-heat and blood stasis, anemopyretic cold, epidemic febrile disease heating.
The Rhizoma Atractylodis Macrocephalae is the dry rhizome of feverfew Rhizoma Atractylodis Macrocephalae Atractylodes macrocephala Koidz.; Bitter in the mouth, sweet, warm in nature; Return spleen, stomach warp; The function invigorating the spleen and benefiting QI, the dampness diuretic, hidroschesis, antiabortive; Cure mainly insufficiency of the spleen lack of appetite, abdominal distention is had loose bowels, phlegm retention vertigo and palpitation, edema, spontaneous perspiration, frequent fetal movement.
Herba Pogostemonis is the dry aerial parts of labiate Herba Pogostemonis Pogostemon cablin (Blanco) Benth.; Acrid in the mouth, slightly warm in nature; Return spleen, stomach, lung meridian; The function eliminating turbid pathogen with aromatics, the appetizing preventing or arresting vomiting is delivered expelling summer-heat; Cure mainly turbid damp obstructing in middle-JIAO, the vomiting of gastral cavity painful abdominal mass, the heat-damp in summer asthenia, uncomfortable in chest easypro, cold-damp is closed summer-heat, and stomachache is vomited and diarrhoea, nasosinusitis headache.
Astragalus polysaccharides is the polyoses extract of the dry root of leguminous plant Radix Astagali Astragalus memeranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao or Radix Astragali Astragalus membranaceus (Fisch.) Bge.; Radix Astragali sweet in the mouth, slightly warm in nature; Return lung, spleen channel; The function invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus, expelling pus and promoting granulation.The modern pharmacological research result shows: astragalus polysaccharides is the main effective site of the Radix Astragali; have raise immunity, induce body to produce multiple pharmacologically actives such as interferon, protection cardiovascular system, blood sugar regulation, blood pressure lowering, wherein particularly evident to the promotion and the regulating action of immunologic function.
In order to make the purpose, technical solutions and advantages of the present invention clearer, will be described in detail the preferred embodiments of the present invention below.
The preparation of the Chinese medicine composition of embodiment 1, anti-enteritis canine parvovirus
The weight proportion of each raw material is: Radix Scutellariae 20g, Radix Sophorae Flavescentis 20g, Radix Pulsatillae 60g, Fructus Gardeniae 25g, Flos Lonicerae 20g, Rhizoma Atractylodis Macrocephalae 15g, Herba Pogostemonis 50g, astragalus polysaccharides 1g.
Preparation method may further comprise the steps:
A, get Herba Pogostemonis, adopt steam distillation to extract volatile oil, add water 500mL and soak after 3 hours, extract volatile oil in 70 ℃ of distilling under reduced pressure of temperature by proportioning, the residue after collecting volatile oil respectively and extracting volatile oil, standby;
B, get Herba Pogostemonis residue after Radix Scutellariae, Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Gardeniae, Flos Lonicerae, the Rhizoma Atractylodis Macrocephalae and step a extract volatile oil, decoct with water and extract three times, add water 1100mL for the first time, soaks after 14 hours and decocted 1 hour, filter collection filtrate by proportioning; For the second time and respectively add water 450mL for the third time, decocted 1.5 hours, filter collection filtrate; Merge three times filtrate, be concentrated into about 1/4th volumes, make the water extract, standby;
C, get step b gained water extract, add astragalus polysaccharides by proportioning, mix homogeneously is concentrated into the thick paste shape, and cooling adds step a gained Herba Pogostemonis Volatile oil, and mix homogeneously adds dextrin and sucrose again, makes granule according to a conventional method.
The preparation of the Chinese medicine composition of embodiment 2, anti-enteritis canine parvovirus
The weight proportion of each raw material is: Radix Scutellariae 60g, Radix Sophorae Flavescentis 25g, Radix Pulsatillae 70g, Fructus Gardeniae 30g, Flos Lonicerae 60g, Rhizoma Atractylodis Macrocephalae 50g, Herba Pogostemonis 60g, astragalus polysaccharides 4g.
Preparation method may further comprise the steps:
A, get Herba Pogostemonis, adopt steam distillation to extract volatile oil, add water 700mL and soak after 2.5 hours, extract volatile oil in 75 ℃ of distilling under reduced pressure of temperature by proportioning, the residue after collecting volatile oil respectively and extracting volatile oil, standby;
B, get Herba Pogostemonis residue after Radix Scutellariae, Flos Lonicerae, Radix Sophorae Flavescentis, the Rhizoma Atractylodis Macrocephalae, the Radix Pulsatillae, Fructus Gardeniae and step a extract volatile oil, decoct with water and extract three times, add water 2200mL for the first time, soaks after 13 hours and decocted 1.5 hours, filter collection filtrate by proportioning; For the second time and respectively add water 800mL for the third time, decocted 1.5 hours, filter collection filtrate; Merge three times filtrate, be concentrated into about 1/4th volumes, make the water extract, standby;
C, get step b gained water extract, add astragalus polysaccharides by proportioning, mix homogeneously is concentrated into the thick paste shape, and cooling adds step a gained Herba Pogostemonis Volatile oil, and mix homogeneously adds dextrin and sucrose again, makes granule according to a conventional method.
The preparation of the Chinese medicine composition of embodiment 3, anti-enteritis canine parvovirus
The weight proportion of each raw material is: Radix Scutellariae 100g, Radix Sophorae Flavescentis 30g, Radix Pulsatillae 80g, Fructus Gardeniae 40g, Flos Lonicerae 100g, Rhizoma Atractylodis Macrocephalae 80g, Herba Pogostemonis 70g, astragalus polysaccharides 2g.
Preparation method may further comprise the steps:
A, get Herba Pogostemonis, adopt steam distillation to extract volatile oil, add water 1000mL and soak after 2 hours, extract volatile oil in 80 ℃ of distilling under reduced pressure of temperature by proportioning, the residue after collecting volatile oil respectively and extracting volatile oil, standby;
B, get Herba Pogostemonis residue after Radix Scutellariae, Flos Lonicerae, Radix Sophorae Flavescentis, the Rhizoma Atractylodis Macrocephalae, the Radix Pulsatillae, Fructus Gardeniae and step a extract volatile oil, decoct with water and extract three times, add water 3500mL for the first time, soaks after 12 hours and decocted 2 hours, filter collection filtrate by proportioning; For the second time and respectively add water 1000mL for the third time, decocted 1 hour, filter collection filtrate; Merge three times filtrate, be concentrated into about 1/4th volumes, make the water extract, standby;
C, get step b gained water extract, add astragalus polysaccharides by proportioning, mix homogeneously is concentrated into the thick paste shape, and cooling adds step a gained Herba Pogostemonis Volatile oil, and mix homogeneously adds dextrin and sucrose again, makes granule according to a conventional method.
The preparation of the Chinese medicine composition of embodiment 4, anti-enteritis canine parvovirus
The weight proportion of each raw material is: Radix Scutellariae 120g, Radix Sophorae Flavescentis 35g, Radix Pulsatillae 90g, Fructus Gardeniae 45g, Flos Lonicerae 120g, Rhizoma Atractylodis Macrocephalae 90g, Herba Pogostemonis 150g, astragalus polysaccharides 6g.
Preparation method may further comprise the steps:
A, get Herba Pogostemonis, adopt steam distillation to extract volatile oil, add water 2000mL and soak after 1.5 hours, extract volatile oil in 85 ℃ of distilling under reduced pressure of temperature by proportioning, the residue after collecting volatile oil respectively and extracting volatile oil, standby;
B, get Herba Pogostemonis residue after Radix Scutellariae, Flos Lonicerae, Radix Sophorae Flavescentis, the Rhizoma Atractylodis Macrocephalae, the Radix Pulsatillae, Fructus Gardeniae and step a extract volatile oil, decoct with water and extract three times, add water 5500mL for the first time, soaks after 11 hours and decocted 2.5 hours, filter collection filtrate by proportioning; For the second time and respectively add water 1500mL for the third time, decocted 0.5 hour, filter collection filtrate; Merge three times filtrate, be concentrated into about 1/4th volumes, make the water extract, standby;
C, get step b gained water extract, add astragalus polysaccharides by proportioning, mix homogeneously is concentrated into the thick paste shape, and cooling adds step a gained Herba Pogostemonis Volatile oil, and mix homogeneously adds dextrin and sucrose again, makes granule according to a conventional method.
The preparation of the Chinese medicine composition of embodiment 5, anti-enteritis canine parvovirus
The weight proportion of each raw material is: Radix Scutellariae 150g, Radix Sophorae Flavescentis 40g, Radix Pulsatillae 100g, Fructus Gardeniae 50g, Flos Lonicerae 150g, Rhizoma Atractylodis Macrocephalae 100g, Herba Pogostemonis 200g, astragalus polysaccharides 10g.
Preparation method may further comprise the steps:
A, get Herba Pogostemonis, adopt steam distillation to extract volatile oil, add water 3000mL and soak after 1 hour, extract volatile oil in 90 ℃ of distilling under reduced pressure of temperature by proportioning, the residue after collecting volatile oil respectively and extracting volatile oil, standby;
B, get Herba Pogostemonis residue after Radix Scutellariae, Flos Lonicerae, Radix Sophorae Flavescentis, the Rhizoma Atractylodis Macrocephalae, the Radix Pulsatillae, Fructus Gardeniae and step a extract volatile oil, decoct with water and extract three times, add water 7900mL for the first time, soaks after 10 hours and decocted 3 hours, filter collection filtrate by proportioning; For the second time and respectively add water 2300mL for the third time, decocted 0.5 hour, filter collection filtrate; Merge three times filtrate, be concentrated into about 1/4th volumes, make the water extract, standby;
C, get step b gained water extract, add astragalus polysaccharides by proportioning, mix homogeneously is concentrated into the thick paste shape, and cooling adds step a gained Herba Pogostemonis Volatile oil, and mix homogeneously adds dextrin and sucrose again, makes granule according to a conventional method.
With reference to embodiment 1 to 5, Chinese medicine composition of the present invention as active component, is added acceptable accessories, conventional formulation method according to pharmaceutical field, can be made into the medicament of various dosage forms,, can reach the object of the invention as tablet, capsule, granule and injection etc.
Chinese medicine composition of the present invention detects through thin layer chromatography, confirms to contain effective ingredient such as baicalin, matrine and Anemonin.
Chinese medicine composition of the present invention is to the prevention effect research of enteritis canine parvovirus
1, Chinese medicine composition of the present invention is to the preventive effect research of enteritis canine parvovirus
Method: the dog (being provided by experimental animal room, school district, Southwestern University Rongchang County) at 30 2～6 monthly ages is divided into 3 groups at random: blank group, model group and administration group, 10 every group; Blank group double injection normal saline every day, each 0.5ml/kg (body weight); Model group is in the preceding not administration of infected dogs parvovirus (being provided by Yunnan University's clinical medicine laboratory); The administration group is from the first seven sky of infected dogs parvovirus, and twice filling every day stomach gives the Chinese medicine composition that embodiment 3 makes, each 1g/kg (body weight); Statistics is respectively organized mortality rate and Chinese medicine composition of the present invention prevention rate to enteritis canine parvovirus.
Result: see Table 1, as seen from table, blank group does not have 1 death at experimental session, model group the 6th day mortality rate behind the infected dogs parvovirus reaches 100%, and administration group the 6th day mortality rate behind the infected dogs parvovirus only is 30%, illustrate that Chinese medicine composition of the present invention can effectively prevent enteritis canine parvovirus, prevention rate reaches 70%.
Table 1 Chinese medicine composition of the present invention is to the preventive effect of enteritis canine parvovirus
2, Chinese medicine composition of the present invention is to the therapeutic effect research of enteritis canine parvovirus
Method: the dog at 30 2～6 monthly ages is divided into 3 groups at random: blank group, model group and administration group, 10 every group; Blank group double injection normal saline every day, each 0.5ml/kg (body weight); Model group not administration behind the infected dogs parvovirus; The administration group is behind the infected dogs parvovirus, and twice filling every day stomach gives the Chinese medicine composition that embodiment 3 makes, each 1g/kg (body weight); Statistics is respectively organized mortality rate and Chinese medicine composition of the present invention treatment effective percentage to enteritis canine parvovirus.
Result: see Table 2, as seen from table, blank group does not have 1 death at experimental session, model group the 6th day mortality rate behind the infected dogs parvovirus reaches 100%, and administration group the 6th day mortality rate behind the infected dogs parvovirus only is 40%, illustrate that Chinese medicine composition of the present invention can effectively treat enteritis canine parvovirus, the treatment effective percentage reaches 60%.
Table 2 Chinese medicine composition of the present invention is to the therapeutic effect of enteritis canine parvovirus
Based on above-mentioned experimental result, can draw as drawing a conclusion: Chinese medicine composition of the present invention can the high-efficiency prevention and control enteritis canine parvovirus, prevention rate reaches 70%, the treatment effective percentage reaches 60%, and make by natural Chinese medicinal herb, toxic and side effects is little, can be used to prepare the anti-enteritis canine parvovirus medicine, the purpose that reaches efficiently, prevents and treats enteritis canine parvovirus safely, economically.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (10)
1. the Chinese medicine composition of anti-enteritis canine parvovirus, it is characterized in that: it is the medicament of being made by the following weight proportion raw material: 20～150 parts of Radix Scutellariaes, 20～40 parts of Radix Sophorae Flavescentiss, 60～100 parts of the Radix Pulsatillaes, 25～50 parts of Fructus Gardeniaes, 20～150 parts of Flos Loniceraes, 15～100 parts of the Rhizoma Atractylodis Macrocephalaes, 50～200 parts of Herba Pogostemonis, 1～10 part of astragalus polysaccharides;
Its preparation method may further comprise the steps:
A, get Herba Pogostemonis, extract volatile oil by proportioning, the residue after collecting volatile oil respectively and extracting volatile oil, standby;
B, get Herba Pogostemonis residue after Radix Scutellariae, Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Gardeniae, Flos Lonicerae, the Rhizoma Atractylodis Macrocephalae and step a extract volatile oil by proportioning, extracting in water filters, and filtrate concentrates, and makes the water extract, and is standby;
C, get step b gained water extract, add astragalus polysaccharides, mix homogeneously by proportioning, be concentrated into the thick paste shape, cooling adds step a gained Herba Pogostemonis Volatile oil, mix homogeneously adds acceptable accessories again, makes medicament by the conventional formulation method of pharmaceutical field.
2. the Chinese medicine composition of anti-enteritis canine parvovirus according to claim 1, it is characterized in that: the weight proportion of described raw material is: 60～120 parts of Radix Scutellariaes, 25～35 parts of Radix Sophorae Flavescentiss, 70～90 parts of the Radix Pulsatillaes, 30～45 parts of Fructus Gardeniaes, 60～120 parts of Flos Loniceraes, 50～90 parts of the Rhizoma Atractylodis Macrocephalaes, 60～150 parts of Herba Pogostemonis, 2～6 parts of astragalus polysaccharidess.
3. the Chinese medicine composition of anti-enteritis canine parvovirus according to claim 2, it is characterized in that: the weight proportion of described raw material is: 100 parts of Radix Scutellariaes, 30 parts of Radix Sophorae Flavescentiss, 80 parts of the Radix Pulsatillaes, 40 parts of Fructus Gardeniaes, 100 parts of Flos Loniceraes, 80 parts of the Rhizoma Atractylodis Macrocephalaes, 70 parts of Herba Pogostemonis, 2 parts of astragalus polysaccharidess.
4. according to the Chinese medicine composition of claim 1,2 or 3 described anti-enteritis canine parvovirus, it is characterized in that: described medicament is a granule.
5. the preparation method of the Chinese medicine composition of the described anti-enteritis canine parvovirus of claim 1 is characterized in that: may further comprise the steps:
A, get Herba Pogostemonis, extract volatile oil by proportioning, the residue after collecting volatile oil respectively and extracting volatile oil, standby;
B, get Herba Pogostemonis residue after Radix Scutellariae, Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Gardeniae, Flos Lonicerae, the Rhizoma Atractylodis Macrocephalae and step a extract volatile oil by proportioning, extracting in water filters, and filtrate concentrates, and makes the water extract, and is standby;
C, get step b gained water extract, add astragalus polysaccharides, mix homogeneously by proportioning, be concentrated into the thick paste shape, cooling adds step a gained Herba Pogostemonis Volatile oil, mix homogeneously adds acceptable accessories again, makes medicament by the conventional formulation method of pharmaceutical field.
6. the preparation method of the Chinese medicine composition of anti-enteritis canine parvovirus according to claim 5, it is characterized in that: adopt steam distillation to extract volatile oil among the step a: add the water logging that is equivalent to 10～15 times of raw material weights and steep after 1～3 hour, extract volatile oil in 70～90 ℃ of distilling under reduced pressure of temperature.
7. the preparation method of the Chinese medicine composition of anti-enteritis canine parvovirus according to claim 5, it is characterized in that: adopt decocting method to prepare the water extract among the step b: decoct with water altogether and extract three times, add the water that is equivalent to 5～10 times of raw material weights for the first time, soak and decocted 1～3 hour after 10～14 hours, filter, collect filtrate; Each adds the water that is equivalent to 2～3 times of raw material weights for the second time and for the third time, decocts 0.5～1.5 hour, filters, and collects filtrate; Merge three times filtrate, concentrate, promptly make the water extract.
8. the preparation method of the Chinese medicine composition of anti-enteritis canine parvovirus according to claim 5, it is characterized in that: medicament described in the step c is a granule.
9. the preparation method of the Chinese medicine composition of anti-enteritis canine parvovirus according to claim 8, it is characterized in that: acceptable accessories described in the step c is dextrin and sucrose.
10. the application of the Chinese medicine composition of the described anti-enteritis canine parvovirus of claim 1 in preparation anti-enteritis canine parvovirus medicine.
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| CN101804140B (en) * | 2010-04-29 | 2011-08-17 | 公安部警犬技术学校 | Oral medicine for preventing and curing canine enteric bacterial disease |
| CN103070949B (en) * | 2013-02-25 | 2014-05-28 | 西南大学 | Traditional Chinese medicament composition and preparation and preparation method thereof |
| CN108030815A (en) * | 2017-12-08 | 2018-05-15 | 重庆昇顺科技有限公司 | The composition, freeze-dried powder and preparation method of a kind of anti-parvovirus of fowl |
| ES2964618T3 (en) * | 2018-01-31 | 2024-04-08 | Sichuan Inlu Weite Pharmaceutical Tech Co Ltd | Saponin B4 from pulsatilla for the treatment of mastitis in dairy cows |
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| CN101057943A (en) * | 2007-05-18 | 2007-10-24 | 赵代良 | Medicine for treating dog parvovirus disease |
| CN101337033A (en) * | 2007-07-08 | 2009-01-07 | 宫兆荣 | Canine parvovirus resisting preparation |
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| CN101057943A (en) * | 2007-05-18 | 2007-10-24 | 赵代良 | Medicine for treating dog parvovirus disease |
| CN101337033A (en) * | 2007-07-08 | 2009-01-07 | 宫兆荣 | Canine parvovirus resisting preparation |
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