CN101503708B - Culture fermentation method of bacillus coagulans antifungal active substance - Google Patents
Culture fermentation method of bacillus coagulans antifungal active substance Download PDFInfo
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- CN101503708B CN101503708B CN2009100680273A CN200910068027A CN101503708B CN 101503708 B CN101503708 B CN 101503708B CN 2009100680273 A CN2009100680273 A CN 2009100680273A CN 200910068027 A CN200910068027 A CN 200910068027A CN 101503708 B CN101503708 B CN 101503708B
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- 241000193749 Bacillus coagulans Species 0.000 title claims abstract description 31
- 229940054340 bacillus coagulans Drugs 0.000 title claims abstract description 31
- 230000004151 fermentation Effects 0.000 title claims abstract description 20
- 238000000855 fermentation Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000013543 active substance Substances 0.000 title claims abstract description 10
- 230000000843 anti-fungal effect Effects 0.000 title abstract 2
- 229940121375 antifungal agent Drugs 0.000 title abstract 2
- 241000894006 Bacteria Species 0.000 claims abstract description 26
- 239000001888 Peptone Substances 0.000 claims abstract description 9
- 108010080698 Peptones Proteins 0.000 claims abstract description 9
- 235000019319 peptone Nutrition 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 7
- 244000068988 Glycine max Species 0.000 claims abstract description 5
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 5
- 239000002609 medium Substances 0.000 claims description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 239000006071 cream Substances 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 8
- 239000006916 nutrient agar Substances 0.000 claims description 7
- 235000016709 nutrition Nutrition 0.000 claims description 7
- 230000035764 nutrition Effects 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 230000001857 anti-mycotic effect Effects 0.000 claims description 5
- 239000002543 antimycotic Substances 0.000 claims description 5
- 229920002261 Corn starch Polymers 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 239000008120 corn starch Substances 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 238000011218 seed culture Methods 0.000 claims description 4
- 238000012807 shake-flask culturing Methods 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 3
- 239000001393 triammonium citrate Substances 0.000 claims description 3
- 235000011046 triammonium citrate Nutrition 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 239000000047 product Substances 0.000 abstract description 16
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 6
- 239000006041 probiotic Substances 0.000 abstract description 6
- 230000000529 probiotic effect Effects 0.000 abstract description 6
- 235000018291 probiotics Nutrition 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 5
- 239000006228 supernatant Substances 0.000 abstract description 5
- 239000003899 bactericide agent Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
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- 241000196324 Embryophyta Species 0.000 abstract description 3
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- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000002028 Biomass Substances 0.000 abstract 1
- 241001052560 Thallis Species 0.000 abstract 1
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 1
- 235000015278 beef Nutrition 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000000417 fungicide Substances 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 239000000575 pesticide Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 240000003768 Solanum lycopersicum Species 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 239000003429 antifungal agent Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 244000000004 fungal plant pathogen Species 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 230000000452 restraining effect Effects 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 1
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- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
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- 108010046845 tryptones Proteins 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for culturing and fermenting an antifungal active substance of Bacillus coagulans, which takes Bacillus coagulans (CGMCC 1.949) as a material to obtain bacteria with higher biomass and antibacterial activity, and fermentation broth obtained by the fermentation process can be used for preparing a bactericide for preventing and treating plant diseases. The invention has the following three advantages: 1. the bacillus coagulans is a probiotic beneficial to human bodies, and the biological bactericide prepared from metabolites of the bacillus coagulans is different from the conventional bactericide in nature and environmental friendliness. 2. In a set of fermentation process established aiming at the bacteria, the soybean cake powder and the corn steep liquor powder are used for replacing beef extract, peptone and the like, so that the production cost is greatly reduced. 3. After the bacteria are fermented, the supernatant of the fermentation product can be used for preparing biological fungicides, and the thalli obtained by centrifugation can be continuously utilized to produce probiotic products, so that the economic benefit is greatly improved.
Description
Technical field
The invention belongs to technical field of bioengineering, relate in particular to (CGMCC1.949) the cultivation and fermentation method of antifungus active substance of a kind of Bacillus coagulans (Bacillus coagulans).
Background technology
Worldwide, agriculture prodn every year, due to illness the Chinese caterpillar fungus evil brought about great losses, and wherein accounted for 80% by fungus-caused disease.For a long time, people mainly adopt chemical pesticide to control stable yields and the high yield of the disease pest and weed of farm crop with the assurance farm crop.But a large amount of uses of chemical pesticide have brought pollution not only for soil, water body and atmosphere; And chemical pesticide is residual in the agricultural byproducts, with the healthy and existence of direct harm humans.Simultaneously, because the use for a long time, in a large number and repeatedly of chemical pesticide, increasing plant pathogenic fungi has produced resistance to chemosynthesis anti-mycotic agent and microbiotic.Therefore, anti-mycotic agent development of new, natural is very necessary.
Bacillus coagulans (Bacillus coagulans) is claimed lactic acid bacillus in the past, is a kind ofly can carry out the lactic acid fermented genus bacillus of homotype.This bacterium also has following good characteristic except that the general advantage with ordinary lactic acid bacteria: (1) facultative aerobic microbiological is easy to cultivate; (2) owing to form gemma, the keeping quality of viable bacteria product is good, can overcome the existing keeping quality of ordinary lactic acid bacteria product poor, prolong the shortcoming that number of viable seriously descends degradation to be difficult to overcome with the shelf time; (3) high temperature resistant, stomach juice-resistant; (5) can in enteron aisle, breed.This bacterium is mainly as probiotic bacterium, to animal disease resistance and the remarkable effect that has that reduces the animal dead rate at present.About the research of this bacterium resisting pathogenic microbes, reported that this bacterium is inhibited to enteron aisle putrefactive bacteriums such as intestinal bacteria, Shigellaes.But do not see that this bacterium is to the inhibited research of fungi report with utilize this bacterium to produce the report of the biological pesticide of anti-Plant diseases fungi.
In the substratum of milk-acid bacterias such as cultivation Bacillus coagulans; Nitrogenous source commonly used is peptone, soy peptone, Tryptones, yeast extract/powder and Carnis Bovis seu Bubali cream, because these material costs are higher, therefore; If can adopt inexpensive nitrogenous source class material to replace above material; Then can reduce the cost that milk-acid bacteria such as Bacillus coagulans produces probiotic composition and production desirable metabolites, especially concerning big production, its meaning is more important.
We find that Bacillus coagulans can produce multiple meta-bolites; These meta-bolitess not only have the number of chemical structure; And in agricultural and foodstuffs industry, have wide biological activity, like stronger antibacterial vigor, for the exploitation of natural biological agricultural chemicals provides new resource.Correlation technique and the document or the patent of method of application of at present, Bacillus coagulans being produced fermentation condition and the fermented product of antifungus active substance do not appeared in the newspapers.
Summary of the invention
The objective of the invention is with Bacillus coagulans CGMCC 1.949 is material, provides a kind of Bacillus coagulans (Bacillus coagulans) to produce the cultivation and fermentation method of antifungus active substance.
Technical scheme of the present invention is:
A kind of cultivation and fermentation method of Bacillus coagulans antimycotics active substance, the step of cultivation and fermentation method is:
(1) inclined-plane seed culture: get ring bacterium mud streak inoculation on nutrition nutrient agar substratum, cultivate 20h down in 40 ℃;
(2) seed shake-flask culture: get ring inclined-plane access and be equipped with in the 250ml triangular flask of 25ml MRS substratum, 40 ℃, 140r/min cultivates 20h, becomes primary seed solution;
(3) shake flask fermentation is cultivated: with 5% inoculum size primary seed solution is inserted and be equipped with in the 500ml triangular flask of 120ml fermention medium, 40 ℃, 180r/min cultivates 18h, becomes secondary seed solution;
(4) fermentor cultivation: secondary seed solution is equipped with in the automatic fermentor tank of fermention medium with the access of 3-6% inoculum size, and main control parameters is following between yeast phase: dissolved oxygen and rotating speed: mixing speed 100-400r/min, and the control dissolved oxygen is 30%～40%; Temperature: 40 ℃, cultivate 48h, can obtain fermented product, fermented product is filtered, remove cell and promptly obtain fermented liquid.
And said Bacillus coagulans (Bacillus coagulans) is preserved in Chinese common micro-organisms preservation administrative center CGMCC, deposit number 1.949.
And, said nutrition nutrient agar substratum: peptone 10.0g, Carnis Bovis seu Bubali cream 3.0g, NaCl 5.0g, agar 15.0g, water 1.0L, pH 7.0; Said MRS substratum: peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract paste 5g, tween-80 1ml, Triammonium citrate 2g, K
2HPO
42g, glucose 20g, sodium acetate 5g, MgSO
47H
2O 0.1g, MnSO
40.05g, water 1.0L, pH7.0; Said fermention medium: soybean cake powder 10.0g, corn starch 10.0g, glucose 6.0g, MgSO
47H
2O 1.0g, K
2HPO
4, MnSO
450ppm, water 1.0L, pH7.0.
Advantage of the present invention and positively effect are:
1. Bacillus coagulans involved in the present invention is one type of probiotic bacterium to the human body beneficial, and the biological bactericide that its meta-bolites is processed is its natural sex and the feature of environmental protection with the maximum difference of sterilant in the past.
2. the present invention is directed in the cover zymotechnique that this bacterium sets up and utilize soybean cake powder and corn starch replacement Carnis Bovis seu Bubali cream, peptone etc., greatly reduce production cost.
3. product bacterium of the present invention by fermentation after, fermentation-product supernatant can be used for preparing biological mycocide, the thalline of centrifugal acquisition can continue utilize to produce probiotic composition, has improved economic interests greatly.
4. technology of the present invention can stably obtain the Bacillus coagulans fermented product, and resulting bacterium has higher living weight and anti-microbial activity, can be used for preparing the controlling plant diseases sterilant, and its production cost is relatively low, is suitable for producing in batches.
Embodiment
Below in conjunction with embodiment the present invention is further specified; Following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
Bacillus coagulans involved in the present invention (Bacillus coagulans) is preserved in Chinese common micro-organisms preservation administrative center (CGMCC), and deposit number is 1.949.
Embodiment 1:
A kind of cultivation and fermentation method of Bacillus coagulans antimycotics active substance, step is:
(1) inclined-plane seed culture: get ring bacterium mud streak inoculation on nutrition nutrient agar substratum, cultivate 20h down in 40 ℃;
(2) seed shake-flask culture: get ring inclined-plane access and be equipped with in the 250ml triangular flask of 25ml MRS substratum, 40 ℃, 140r/min cultivates 20h, becomes primary seed solution;
(3) shake flask fermentation is cultivated: with 5% inoculum size primary seed solution is inserted and be equipped with in the 500ml triangular flask of 120ml fermention medium, 40 ℃, 180r/min cultivates 18h, becomes secondary seed solution;
(4) 5L fermentor cultivation: secondary seed solution is equipped with in the automatic fermentor tank of 5L of 3.5L fermention medium with the access of 5% inoculum size, and setting liquid amount is 70%, and initial mixing speed is 100r/min, and air flow is 1: 1, and decide dissolved oxygen this moment is 100%; Reduce to 30% when following when dissolved oxygen, improve rotating speed with the control dissolved oxygen more than 30%, temperature is cultivated 48h for 40 ℃, can obtain fermented product, and fermented product is filtered, and removes cell, can obtain fermented liquid.
The formation of each substratum is in the present embodiment:
Nutrition nutrient agar substratum: peptone 10.0g, Carnis Bovis seu Bubali cream 3.0g, NaCl 5.0g, agar 15.0g, water 1.0L, pH 7.0;
MRS substratum: peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract paste 5g, tween-80 1ml, Triammonium citrate 2g, K
2HPO
42g, glucose 20g, sodium acetate 5g, MgSO
47H
2O 0.1g, MnSO
40.05g, water 1.0L, pH7.0;
Fermention medium: soybean cake powder 10.0g, corn starch 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
4, MnSO
450ppm, water 1.0L, pH7.0.
Embodiment 2:
(1) inclined-plane seed culture: get ring bacterium mud streak inoculation on nutrition nutrient agar substratum, cultivate 20h down in 40 ℃;
(2) seed shake-flask culture: get ring inclined-plane access and be equipped with in the 250ml triangular flask of 25mlMRS substratum, 40 ℃, 140r/min cultivates 20h, becomes primary seed solution;
(3) shake flask fermentation is cultivated: with 5% inoculum size primary seed solution is inserted and be equipped with in the 500ml triangular flask of 120ml fermention medium, 40 ℃, 180r/min cultivates 18h, becomes secondary seed solution;
(4) 30L fermentor cultivation: secondary seed solution is inserted in the automatic fermentor tank of 30L that the 21L fermention medium is housed (liquid amount is 70%) with 3.6% inoculum size, and initial mixing speed is 100r/min, and air flow is 1: 0.3, and decide dissolved oxygen this moment is 100%.Reduce to 30% when following when dissolved oxygen, improve rotating speed with the control dissolved oxygen more than 30%, temperature is cultivated 48h for 40 ℃, can obtain fermented product, and fermented product is filtered, and removes cell and promptly obtains fermented liquid.
The formation of each substratum is identical with embodiment 1 in the present embodiment.
Narrate stripped bacteriostatic test of the present invention (growth velocity inhibition method) below, the application of Bacillus coagulans fermented liquid is on the basis of embodiment 1,2, to carry out:
1, with the fermented liquid that obtains in the technology further at 14000r/min, 4 ℃ of centrifugal 10min, collect supernatant.
2, in the 9ml PDA substratum that melts, adding the 1ml supernatant, the 10ml substratum that obtains is poured in the sterilization petridish of diameter 9.0cm, process flat board, is contrast with the PDA that adds aseptic culture medium.From cultivating the plant pathogenic fungi colony edge of 3～6d, be cut into the bacterium piece of diameter 5mm with punch tool, place dull and stereotyped central authorities, cultivate 6d in 24 ℃ of incubators, measure the pathogenic bacteria colony diameter;
3, from following table, can see: Bacillus coagulans fermented product supernatant 100～200ml (fermented liquid)/L to tomato early the mycelial growth of vaccine, tomato gray mould, cucumber phytophthora, muskmelon Fusarium oxysporum, apple anthrax-bacilus stronger restraining effect is arranged; Inhibiting rate is 60～100%, and wherein fermented product is the highest to the restraining effect of tomato gray mould mycelial growth.
Claims (2)
1. the cultivation and fermentation method of a Bacillus coagulans antimycotics active substance, it is characterized in that: the step of cultivation and fermentation method is:
(1) inclined-plane seed culture: get ring bacterium mud streak inoculation on nutrition nutrient agar substratum, cultivate 20h down in 40 ℃;
(2) seed shake-flask culture: get ring inclined-plane access and be equipped with in the 250ml triangular flask of 25ml MRS substratum, 40 ℃, 140r/min cultivates 20h, becomes primary seed solution;
(3) shake flask fermentation is cultivated: with 5% inoculum size primary seed solution is inserted and be equipped with in the 500ml triangular flask of 120ml fermention medium, 40 ℃, 180r/min cultivates 18h, becomes secondary seed solution;
(4) fermentor cultivation: secondary seed solution is equipped with in the automatic fermentor tank of fermention medium with the access of 3-6% inoculum size, and main control parameters is following between yeast phase: dissolved oxygen and rotating speed: mixing speed 100-400r/min, and the control dissolved oxygen is 30%～40%; Temperature: 40 ℃, cultivate 48h, can obtain fermented product, fermented product is filtered, remove cell and promptly obtain fermented liquid,
Said Bacillus coagulans (Bacillus coagulans) is preserved in Chinese common micro-organisms preservation administrative center CGMCC, deposit number 1.949.
2. the cultivation and fermentation method of a kind of Bacillus coagulans antimycotics active substance according to claim 1 is characterized in that: said nutrition nutrient agar substratum: peptone 10.0g, Carnis Bovis seu Bubali cream 3.0g; NaCl 5.0g; Agar 15.0g, water 1.0L, pH 7.0; Said MRS substratum: peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract paste 5g, tween-80 1ml, Triammonium citrate 2g, K
2HPO
42g, glucose 20g, sodium acetate 5g, MgSO
47H
2O 0.1g, MnSO
40.05g, water 1.0L, pH7.0; Said fermention medium: soybean cake powder 10.0g, corn starch 10.0g, glucose 6.0g, MgSO
47H
2O 1.0g, K
2HPO
4, MnSO
450ppm, water 1.0L, pH7.0.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101775360B (en) * | 2009-09-04 | 2012-02-01 | 武汉科诺生物科技股份有限公司 | Preparation method of Bacillus licheniformis and raw materials |
| CN101948783B (en) * | 2010-08-31 | 2012-03-21 | 广州广牧丰生物技术有限公司 | Culture medium capable of improving viable count of lactic acid bacillus |
| CN103300126B (en) * | 2013-06-07 | 2014-11-26 | 浙江工商大学 | Application of bacillus coagulans serving as biological preservative in preservation and fresh keeping of pseudosciaena crocea |
| US9596861B2 (en) * | 2013-12-24 | 2017-03-21 | Sami Labs Limited | Method of producing partially purified extracellular metabolite products from Bacillus coagulans and biological applications thereof |
| CN104738093A (en) * | 2015-03-25 | 2015-07-01 | 南京工业大学 | Preparation method of bacillus coagulans bacterial suspension |
| CN106191178B (en) * | 2016-08-25 | 2019-05-10 | 江南大学 | Method for producing bacteriostatic active substance by using bacillus coagulans |
| JP2020509749A (en) * | 2017-03-08 | 2020-04-02 | シンバイオシス インターナショナル ユニバーシティーＳｙｍｂｉｏｓｉｓ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｕｎｉｖｅｒｓｉｔｙ | Methods for inducing spore formation in Bacillus coagulans |
| CN108102967A (en) * | 2018-01-11 | 2018-06-01 | 天津生机集团股份有限公司 | One breeder source coagulating bacillus strain and its production spore method |
| CN108721336A (en) * | 2018-06-15 | 2018-11-02 | 江苏远山生物技术有限公司 | The preparation method and application of bacillus coagulans and bacillus licheniformis cocktail spray |
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| CN1472327A (en) * | 2003-06-25 | 2004-02-04 | 中国科学院沈阳应用生态研究所 | Fermentation of antifungal antibiotic produced by oceanic bacillus |
| CN1952117A (en) * | 2006-04-18 | 2007-04-25 | 兰州大学 | Bacillus subtilis strain and application thereof |
| CN101173242A (en) * | 2007-10-18 | 2008-05-07 | 中国科学院微生物研究所 | Method for producing L-lactic acid and coagulate bacillus cereus special for the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1472327A (en) * | 2003-06-25 | 2004-02-04 | 中国科学院沈阳应用生态研究所 | Fermentation of antifungal antibiotic produced by oceanic bacillus |
| CN1952117A (en) * | 2006-04-18 | 2007-04-25 | 兰州大学 | Bacillus subtilis strain and application thereof |
| CN101173242A (en) * | 2007-10-18 | 2008-05-07 | 中国科学院微生物研究所 | Method for producing L-lactic acid and coagulate bacillus cereus special for the same |
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