CN101411920B - Multichannel micro-current stimulator in vitro - Google Patents
Multichannel micro-current stimulator in vitro Download PDFInfo
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- CN101411920B CN101411920B CN2008102034454A CN200810203445A CN101411920B CN 101411920 B CN101411920 B CN 101411920B CN 2008102034454 A CN2008102034454 A CN 2008102034454A CN 200810203445 A CN200810203445 A CN 200810203445A CN 101411920 B CN101411920 B CN 101411920B
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Abstract
The invention discloses an in vitro multi-channel micro-current stimulator used in the field of an artificial nerve repair technology, which comprises a pulse signal generator, voltage-current conversion circuits, and a multi-channel connector, wherein the output end of the pulse signal generator is connected with input ends of the voltage-current conversion circuits, output ends of the voltage-current conversion circuits are all connected with the multi-channel connector, the pulse signal generator generates a single-phase pulse square-wave voltage signal and outputs the signal to the voltage-current conversion circuits, and the voltage-current conversion circuits convert the single-phase pulse square-wave voltage signal into a single-phase pulse current signal; and when the voltage-current conversion circuits convert the single-phase pulse square-wave voltage signal into the single-phase pulse current signal, the frequency and the pulse width of pulse are adjusted through the pulse signal generator, and the pulse amplitude is adjusted through a variable resistor Rref. The frequency, the pulse width, and the amplitude output by the in vitro multi-channel micro-current stimulator have larger adjustment range, and a device is easy to manufacture and has lower cost.
Description
Technical field
The present invention relates to a kind of device that is used for artificial neuron recovery technique field, particularly a kind of multichannel micro-current stimulator in vitro.
Background technology
Neural reparation is an importance in the neural engineering research field, mainly comprises sensory function (audition, vision etc.) reparation and motor function reparation, has begun to be applied to clinical research, and has obtained some gratifying progress.In deep brain stimulation (Deep Brain Stimulation:DBS) research, stimulate the corresponding site of brain, can suppress abnormal neural activity, make parkinsonism (Pakinson Disease:PD) patient can control their behavior as the normal person; Adopt implanted neural microelectrode effective stimulus vagus nerve, can treat epileptics, reduce induced seizures cycle, intensity of attack and persistent period greatly.In artificial hearing reparation research, stimulate remaining acoustic fibers by micro-current stimulator, provide electroneurographic signal by pathway to the brain auditory center, produce auditory response.Commercial artificial cochlea's product has made thousands upon thousands deafness recover audition.The artificial vision repairs by vision prosthesis and makes those because disease or wound cause the people who loses one's sight to produce vision, and the blind person can produce light sensation at present, and can recognize simple alphabetical figure.The motor function reparation is biological nerve-musculature to be applied electrical stimulation signal substitute the control command of being sent by brain or spinal cord originally.Medically adopt the Therapeutic Method of functional electrical stimulation (Functional Electrical Stimulation:FES) at present, recover paralytic's neuromuscular function thereby utilize body surface or Endermic implantating microelectrode that muscle is applied electricity irritation, successfully be used to help the paralytic to recover grasped, urinate, stand even the function of walking.The nerve reparation in above-mentioned each field research is based on the electricity irritation to biological tissue, so multichannel micro-current stimulator is to carry out the neural critical component of repairing, and has important researching value and clinical meaning.
Find by prior art documents, Chinese patent application number is 200510053427.9, the open date is: 2006.09.13, patent name is: " a kind of little function of current is in the method and apparatus of eyeball ", this invention provides a kind of little function of current in the method and apparatus of eyeball, and uses the encapsulating material of the epoxy resin of modification as electrode.The device of this invention has: a holding wire, a media units couple this holding wire, a main frame couples this holding wire, exports this little electric current.The frequency range of the output frequency of this device is between 8Hz to 27Hz, and the amplitude of this little electric current is between 10 μ A to 2000 μ A.Its deficiency is: 1, Shu Chu stimulating current is a single channel, fails to stimulate fully peripheral neurocyte; 2, the little power frequency adjustable extent of output is narrower, is not suitable for the stimulation work of other neurocytes.
Summary of the invention
The present invention is directed to above-mentioned the deficiencies in the prior art, a kind of multichannel micro-current stimulator in vitro has been proposed, the present invention can be used in the microelectrode that the artificial neuron reparation is used and carries out the fatigability test, and this multichannel micro-current stimulator has characteristics such as circuit structure is simple, easy to adjust, precision is high, application is wide.
The present invention is achieved through the following technical solutions, the present invention includes: pulse signal generator, voltage-current converter circuit, multichannel adapter, wherein:
The outfan of pulse signal generator links to each other with the input of several voltage-current converter circuits, the outfan of several voltage-current converter circuits all links to each other with the multichannel adapter, pulse signal generator produces the monophasic pulses if square wave voltage signal, and export to voltage-current converter circuit, voltage-current converter circuit is converted to the monophasic pulses if current signal with the monophasic pulses if square wave voltage signal.
Described voltage-current converter circuit comprises: first resistance R 1, second resistance R 2, the 3rd resistance R 3, the 4th resistance R 4, variable resistance R
Ref, the first operational amplifier A P1, the second operational amplifier A P2, load resistance Ri, wherein,
One end ground connection of first resistance R 1, the other end links to each other with the negative pole of the first operational amplifier A P1, one end of second resistance R 2 links to each other with the outfan of pulse signal generator, the other end links to each other with anodal and the 4th resistance R 4 of the first operational amplifier A P1 respectively, be connected to outfan and the variable resistance R of the 3rd resistance R 3, the first operational amplifier A P1 between the outfan of the first operational amplifier A P1 and the negative pole
RefAn end link to each other, the 4th resistance R 4 links to each other with the outfan of the second operational amplifier A P2, the negative pole of the second operational amplifier A P2 links to each other with outfan, positive pole and variable resistance R
RefThe other end link to each other, and by load resistance Ri ground connection, variable resistance R
RefAnd between the load resistance Ri current output terminal.
Described variable resistance R
Ref, its change in resistance scope is 0-100k Ω.
Described first resistance R 1 is identical with second resistance R, 2 resistances, and the 3rd resistance R 3 is identical with the 4th resistance R 4 resistances.
When the present invention works, pulse signal generator produces the monophasic pulses if square wave voltage signal, and export to voltage-current converter circuit, voltage-current converter circuit is converted to the monophasic pulses if current signal with the monophasic pulses if square wave voltage signal, thereby produce the adjustable single-phase little current impulse of multichannel parameter, frequency, the pulsewidth of pulse are regulated by pulse signal generator, and pulse amplitude is by variable resistance R
RefRegulate.
Compared with prior art, the present invention has following beneficial effect: (1) apparatus of the present invention combine with the microelectrode device to be mainly used in the nerve stimulation microelectrode are carried out external electricity fatigability test; (2) each passage can stimulate simultaneously, and output parameter is relatively independent, and is easy to adjust: compare with the control of existing large program, this device can be directly manually in the amplitude of pulse signal generator adjusted pulse frequency, pulse width and pulse; (3) output frequency, pulsewidth, amplitude adjusted scope are bigger: compare with existing invention, the output pulse frequency of this invention is at 500 μ Hz to 50MHz, and pulse width is adjustable at 8.0ns to 1999.9ns, and pulse amplitude is adjustable at 10mVpp to 10Vpp; (4) the present invention is easy to making, cost is lower.Do not need to comprise host computer, connecting line is simple; Than a large amount of control sequences, all adjustings of apparatus of the present invention all can manually be controlled, and are easy to operate; The real-time waveform result of utilization oscillograph observation is clear simple.
Description of drawings
Fig. 1 is a system architecture diagram of the present invention;
Fig. 2 is the circuit diagram of voltage-current converter circuit of the present invention;
The current waveform sketch map of Fig. 3 for exporting in the embodiments of the invention.
The specific embodiment
Below in conjunction with accompanying drawing embodiments of the invention are elaborated: present embodiment has provided detailed embodiment and process being to implement under the prerequisite with the technical solution of the present invention, but protection scope of the present invention is not limited to following embodiment.
Present embodiment is used to implant under the interior retina of animal body, the retina or the external fatigability test of the multicenter film membrane electrode of epichoroidal space.
As shown in Figure 1, present embodiment comprises: pulse signal generator, voltage-current converter circuit, multichannel adapter, wherein:
The outfan of pulse signal generator links to each other with the input of several voltage-current converter circuits, the outfan of several voltage-current converter circuits all links to each other with the multichannel adapter, pulse signal generator produces the monophasic pulses if square wave voltage signal, and export to voltage-current converter circuit, voltage-current converter circuit is converted to the monophasic pulses if current signal with the monophasic pulses if square wave voltage signal.
As shown in Figure 2, described voltage-current converter circuit comprises: first resistance R 1, second resistance R 2, the 3rd resistance R 3, the 4th resistance R 4, variable resistance R
Ref, the first operational amplifier A P1, the second operational amplifier A P2, load resistance Ri, wherein,
One end ground connection of first resistance R 1, the other end links to each other with the negative pole of the first operational amplifier A P1, one end of second resistance R 2 links to each other with the outfan of pulse signal generator, the other end links to each other with anodal and the 4th resistance R 4 of the first operational amplifier A P1 respectively, be connected to outfan and the variable resistance R of the 3rd resistance R 3, the first operational amplifier A P1 between the outfan of the first operational amplifier A P1 and the negative pole
RefAn end link to each other, the 4th resistance R 4 links to each other with the outfan of the second operational amplifier A P2, the negative pole of the second operational amplifier A P2 links to each other with outfan, positive pole and variable resistance R
RefThe other end link to each other, and by load resistance Ri ground connection, variable resistance R
RefAnd between the load resistance Ri current output terminal.
Described variable resistance R
Ref,, its change in resistance scope is 0-100k Ω.
Described first resistance R 1 is identical with second resistance R, 2 resistances, and the 3rd resistance R 3 is identical with the 4th resistance R 4 resistances, and resistance is respectively: R1=R2=1k Ω, R3=R4=3.1k Ω.
During present embodiment work, pulse signal generator produces the monophasic pulses if square wave voltage signal, and export to voltage-current converter circuit, voltage-current converter circuit is converted to the monophasic pulses if current signal with the monophasic pulses if square wave voltage signal, thereby produce the adjustable single-phase little current impulse of multichannel parameter, frequency, the pulsewidth of pulse are regulated by pulse signal generator, and pulse amplitude is by variable resistance R
RefRegulate.
Pulse signal generator model in the present embodiment is 33250A, and the model of the first operational amplifier A P1, the second operational amplifier A P2 is TL084.
The monophasic pulses if square-wave signal v of pulse signal generator output frequency 1kHz
0, amplitude is 1V, is frequency 1kHz for making output signal, amplitude is the monophasic pulses if current signal of 100 μ A, according to formula:
Wherein, v
0Be the output voltage signal of pulse signal generator, choose adjustable resistance R
RefResistance be 33k Ω, then amplitude output signal approximately is adjusted to about 100 μ A, satisfies the requirement that vision is repaired.
As shown in Figure 3, be the output current wave sketch map of present embodiment, the little electric current among the figure is a monophasic pulses if, and amplitude is 100 μ A, and pulsewidth is 0.5ms, and frequency is 1kHz.Just can carry out secular fatigability electricity irritation to multichannel microelectrode array with this multichannel micro-current stimulator, the long-term work situation in vivo of assessment microelectrode array.
In the present embodiment, the pulse frequency of pulse-pattern generator is between 500 μ Hz to 50MHz, thereby above-mentioned little current pulse frequency scope is adjustable at 500 μ Hz to 50MH, regulate little electric current output waveform at 1kHz, output waveform is stable, can carry out fatigability test experiments steady in a long-term to multichannel microelectrode.
In the present embodiment, the pulse amplitude of pulse-pattern generator is adjustable at 10mVpp to 10Vpp, pass through voltage-current converter circuit, by regulating slide rheostat, available current range is adjustable at 0.31 μ A to 3A, for satisfy vision repair on the retina, retina down and epichoroidal space to the requirement of microelectric current, regulate little electric current continually and steadily at 100 μ A, microelectrode is carried out fatigability steady in a long-term to stimulate.
Claims (3)
1. a multichannel micro-current stimulator in vitro is characterized in that, comprising: pulse signal generator, voltage-current converter circuit, multichannel adapter, wherein:
The outfan of pulse signal generator links to each other with the input of several voltage-current converter circuits, the outfan of several voltage-current converter circuits all links to each other with the multichannel adapter, pulse signal generator produces the monophasic pulses if square wave voltage signal, and export to voltage-current converter circuit, voltage-current converter circuit is converted to the monophasic pulses if current signal with the monophasic pulses if square wave voltage signal;
Described voltage-current converter circuit comprises: first resistance (R1), second resistance (R2), the 3rd resistance (R3), the 4th resistance (R4), variable resistance (R
Ref), first operational amplifier (AP1), second operational amplifier (AP2), load resistance (Ri), wherein,
One end ground connection of first resistance (R1), the other end links to each other with the negative pole of first operational amplifier (AP1), one end of second resistance (R2) links to each other with the outfan of pulse signal generator, the other end links to each other with anodal and the 4th resistance (R4) of first operational amplifier (AP1) respectively, be connected to the 3rd resistance (R3) between the outfan of first operational amplifier (AP1) and the negative pole, the outfan of first operational amplifier (AP1) and variable resistance (R
Ref) an end link to each other, the 4th resistance (R4) links to each other with the outfan of second operational amplifier (AP2), the negative pole of second operational amplifier (AP2) links to each other with the outfan of second operational amplifier (AP2), the positive pole of second operational amplifier (AP2) and variable resistance (R
Ref) the other end link to each other, and by load resistance (Ri) ground connection, variable resistance (R
Ref) and load resistance (Ri) between be current output terminal.
2. multichannel micro-current stimulator in vitro according to claim 1 is characterized in that, described variable resistance (R
Ref), its change in resistance scope is 0-100k Ω.
3. multichannel micro-current stimulator in vitro according to claim 1 is characterized in that, described first resistance (R1) is identical with second resistance (R2) resistance, and the 3rd resistance (R3) is identical with the 4th resistance (R4) resistance.
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| JP6258958B2 (en) * | 2012-11-27 | 2018-01-10 | コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． | Electrical multi-channel system, especially electrical multi-channel system for nerve stimulation |
| CN103479449B (en) * | 2013-09-17 | 2016-02-24 | 深圳先进技术研究院 | Acquired blindness human brain marine origin is as the system and method in the perception external world |
| CN109173056B (en) * | 2018-10-19 | 2022-03-22 | 南通大学 | Rat spinal cord neural signal detection and evaluation system and method |
| WO2020227990A1 (en) * | 2019-05-15 | 2020-11-19 | 张东升 | Multi-dimensional meridian balancing instrument |
| CN114733067B (en) * | 2022-05-18 | 2023-08-18 | 首都医科大学宣武医院 | Non-implanted multi-target feedback type electric stimulator |
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