CN101248870A - Lowering blood and blood pressure plants extract, preparation and applications thereof - Google Patents
Lowering blood and blood pressure plants extract, preparation and applications thereof Download PDFInfo
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- CN101248870A CN101248870A CNA2008100609226A CN200810060922A CN101248870A CN 101248870 A CN101248870 A CN 101248870A CN A2008100609226 A CNA2008100609226 A CN A2008100609226A CN 200810060922 A CN200810060922 A CN 200810060922A CN 101248870 A CN101248870 A CN 101248870A
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- 230000036772 blood pressure Effects 0.000 title abstract description 36
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- NTDLXWMIWOECHG-YRCFQSNFSA-N apiin Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O[C@H]1[C@@H]([C@@](O)(CO)CO1)O)O)CO)C(C=1)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C=C1 NTDLXWMIWOECHG-YRCFQSNFSA-N 0.000 description 13
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- PEFASEPMJYRQBW-UHFFFAOYSA-N kaempferol 7-O-alpha-L-rhamnopyradoside 3-O-beta-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(OC4C(C(O)C(O)C(C)O4)O)C=C3OC=2C=2C=CC(O)=CC=2)=O)O1 PEFASEPMJYRQBW-UHFFFAOYSA-N 0.000 description 13
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Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides plant extract with blood lipid reducing and blood pressure lowering effects, and a preparation method and an application thereof. The preparation method comprises the steps of mixing the flower and/or the flower bud of Chrysanthemum morifolium, extracting with 50-80% (v/v) aqueous ethanol at 60-100 DEG C for 1-3 hours to obtain extractive solution, adsorbing with macroporous adsorption resin, eluting with 60-90% (v/v) aqueous ethanol, collecting eluate, and drying until water content is lower than 12% to obtain the plant extract. The inventive extract contains more than 50% of effective ingredients such as galuteolin, celereoside, luteolin, apigenin, acacetin-7-rhamnosidgluoside, acacipetalin, flavonoid glycoside, and chlorogenic acid; and has blood lipid reducing and blood pressure lowering effects. Compared with the existing domestic and overseas products, the inventive extract has more complete efficacy, and more ideal effects in restoring normal blood lipid level and blood pressure level for demanded people.
Description
(1) technical field
The present invention relates to hypotensive plant extracts of a kind of reducing blood lipid and preparation method thereof, with and application in preparation reducing blood lipid hypotensive double effects functional food.
(2) background technology
A large amount of crowd have blood fat, blood sugar, the higher physiological phenomenon of blood pressure both at home and abroad, very need be the functional food conditioning of raw material with the natural plants, the functional food long period that particularly with the food resource is development of raw materials is taken conditioning, thereby this type of functional food huge market demand.At present, the higher people of blood fat is often higher or blood pressure is higher with blood sugar, the all higher people of blood fat, blood sugar and blood pressure is also many, but existing product is the simple function product, thereby be starved of development and have the auxiliary antilipemic of multi-efficiency, hypoglycemic, antihypertensive function food, supply the market is to improve the popular general level of the health.
The extract of chrysanthemum rice or chrysanthemum there are some researches show, and it has and strengthens the human body coronary blood flow, improves function such as the rhythm of the heart, and its composition is galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and chlorogenic acid.But purified extract is not developed as the functional food of tool auxiliary antilipemic, hypotensive double effects.
(3) summary of the invention
The present invention seeks at above-mentioned present situation, the functional food of research and development tool auxiliary antilipemic, hypotensive double effects is for improving the health of masses service.
The technical solution used in the present invention is:
The hypotensive plant extracts of a kind of reducing blood lipid, obtained by following method: the mixture with chrysanthemum or chrysanthemum rice or chrysanthemum and chrysanthemum rice is a raw material, pulverizes, and adds the ethanol water of 50～80% volumetric concentrations of 4～7 times of quality, 60～100 ℃ of insulations were extracted 1～3 hour, filtration obtains extract, and extract is added ethanol to ethanol volumetric concentration more than 75%, stirs, left standstill 0.5～2 hour, filter once more, get filtrate recycling ethanol, concentrate paste; Macroporous absorbent resin post on the paste, elder generation's distilled water drip washing, again with the ethanol water drip washing of 10～20% volumetric concentrations, remove impurity, last ethanol water wash-out with 60～90% volumetric concentrations, collect eluent and reclaim ethanol, be dried to water content below 12%, get the hypotensive plant extracts of described reducing blood lipid, galuteolin wherein, apiin, cyanidenon, apiolin, the robinin glucoside, acacipetalin, flavonoid glycoside and eight kinds of material gross masses of chlorogenic acid content are more than 50%, this also is that the present invention will put the place, experimental results show that, only prepare according to the inventive method, eight kinds of material gross mass content that purifying obtained are at the extract more than 50%, just has tangible reducing blood lipid blood pressure reduction effect, and, then can not reach the requirement hypotensive of functions of the state food to reducing blood lipid by above-mentioned eight kinds of extracts that content of material is lower that other method obtains, more do not have tangible reducing blood lipid blood pressure reduction effect.
The detection method of galuteolin, apiin, cyanidenon, apiolin is: precision takes by weighing alkaloidal standard items, with the dissolving of 70% volumetric concentration ethanol, adds 5 H
2SO
4, shake up, promptly get standard liquid.The accurate standard liquid of drawing adds bromocresol green, and the pH4.0 buffer solution is used dichloromethane extraction 2 times, and the 2min that at every turn vibrates leaves standstill 30min, combining extraction liquid, and the carrene constant volume is blank with the carrene, wavelength 414nm place surveys absorbance, makes calibration curve.Take by weighing extract, with the dissolving of 70% volumetric concentration ethanol, as liquid to be measured.Accurately draw liquid to be measured, press the operation of calibration curve detection method, be determined at the absorbance at 414nm place, calculate the total content of galuteolin, apiin, cyanidenon, apiolin in the extract according to calibration curve.
The detection method of robinin glucoside, acacipetalin, flavonoid glycoside and chlorogenic acid is: with the rutin standard items, and 10% volumetric concentration ethanol ultrasonic dissolution, preparing standard solution.Add 5% mass concentration NaNO
2The aqueous solution, leave standstill 6Min, add 10% mass concentration Al (NO
3)
3, the aqueous solution, leave standstill 6Min, add the NaOH of 1mol/l concentration, add the ethanol of 30% volumetric concentration, left standstill 10 minutes.Solution with no rutin is blank, measures trap at the 510nm place, makes calibration curve.Accurately take by weighing extract, be dissolved in the 50% volumetric concentration ethanol, as liquid to be measured.Accurately draw liquid to be measured, press the operation of calibration curve detection method, measure its absorbance, calculate the total content of robinin glucoside, acacipetalin, flavonoid glycoside and chlorogenic acid in the extract according to calibration curve at the 510nm place.
Extract of the present invention be raw material through the specific concentrations alcohol extract, again through macroporous absorbent resin absorption, after the component that the specific concentrations ethanol elution obtains, and active ingredient is basic identical in chrysanthemum and the chrysanthemum rice, therefore can be raw material with chrysanthemum or chrysanthemum rice separately, also can chrysanthemum and the mixture of chrysanthemum rice arbitrary proportion be raw material.After described chrysanthemum rice or chrysanthemum can extract separately, extract utilized respectively, also can mix the back and utilize.Chrysanthemum rice and chrysanthemum also can mix earlier, and co-extracted obtains plant extracts again.
The invention still further relates to the method for the hypotensive plant extracts of the described reducing blood lipid of preparation, described method comprises: the mixture with chrysanthemum or chrysanthemum rice or chrysanthemum and chrysanthemum rice is a raw material, pulverize, add the ethanol water of 50～80% volumetric concentrations of 4～7 times of quality, 60～100 ℃ of temperature were soaked 1～3 hour, filtration obtains extract: adjust ethanol volumetric concentration to 75%～80% in the extract, stir, left standstill 0.5～2 hour, filter once more, get filtrate recycling ethanol, concentrate paste; Macroporous absorbent resin post on the paste, elder generation's distilled water drip washing, remove impurity with the ethanol water drip washing of 10～20% volumetric concentrations again, last ethanol water wash-out with 60～90% volumetric concentrations, collect eluent and reclaim ethanol, be dried to water content below 12%, get the hypotensive plant extracts of described reducing blood lipid.
Macroporous absorbent resin claims polymer absorbant again, mostly is white spheric granules greatly, has the high molecular polymer of three-dimensional space net structure, physicochemical property is stable, be insoluble to acid, alkali and organic solvent, better to organic selectivity, be not subjected to the influence of inorganic salts and strong ion low molecular compound.Macroporous absorbent resin is for absorption and screen the parting material that principle combines.Its main feature is porous and higher specific surface area.Can be divided into nonpolar, Semi-polarity, polarity and strong polarity several types at present polymeric adsorbent by its surface nature, commonly used is styrene type, acrylonitrile type and acrylic ester type etc.
Among the present invention, described macroporous absorbent resin is preferably styrene tyle macroporous adsorption resin, and model is preferably AB-8.
Described macroporous absorbent resin is with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours, again with the distilled water washing, drain back dress post the medicinal extract thing adsorbed.
The ethanol water volumetric usage of described 60～90% volumetric concentrations is 3～5 times of macroporous absorbent resin volume, elution flow rate 1.5～4L/min.
Preferably, described method is as follows: the mixture with chrysanthemum or chrysanthemum rice or chrysanthemum and chrysanthemum rice is a raw material, pulverizes, the ethanol water that adds 50～70% volumetric concentrations of 4～7 times of quality, 60～100 ℃ of insulations were extracted 1～3 hour, filtered and obtained extract, regulated ethanol to ethanol volumetric concentration 75%～80%, stir, left standstill 0.5～2 hour, and filtered once more, get filtrate recycling ethanol, being concentrated into relative density is 1.10～1.15, gets paste; Get macroporous absorbent resin with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours, again with distilled water washing three times, drain, get the amount dress post of 1L macroporous absorbent resin (with wet resin) in 2.0～4.0g medicinal extract thing, macroporous absorbent resin post on the paste, elder generation is with the distilled water drip washing of 2 times of volumes of macroporous absorbent resin, remove impurity with the ethanol water drip washing of 10～20% volumetric concentrations of 1 times of volume of macroporous absorbent resin again, at last with the ethanol water wash-out of 60～90% volumetric concentrations of 3.5～4.5 times of volumes of macroporous absorbent resin, collect the eluent vacuum and reclaim ethanol for 50 ℃, be dried to water content below 12%, get the hypotensive plant extracts of described reducing blood lipid.
The present invention is with chrysanthemum rice and/or the contained galuteolin of chrysanthemum, apiin, cyanidenon, apiolin, the robinin glucoside, acacipetalin, functional component such as flavonoid glycoside and chlorogenic acid extracts and is purified to total content more than 50%, have desirable auxiliary antilipemic and hypotensive double effects, auxiliary antilipemic, hypotensive effect reaches the standard of functions of the state food, chrysanthemum rice or chrysanthemum extract are become have the desirable feedstock of auxiliary antilipemic and hypotensive double effects functional food, significantly improve the value of chrysanthemum rice and chrysanthemum, do not had research to carry out and production so far.The functional food with auxiliary antilipemic and hypotensive double effects that chrysanthemum rice and chrysanthemum extract are made compares with domestic and international existing like product, and effect is more outstanding, and is better to demand crowd's recovery normal lipid, blood pressure effect.
By the test comparative measurements, the hypotensive plant extracts of reducing blood lipid of the present invention on average descends than control group and reaches more than 10% feeding hyperlipemia rat every day the test group serum total cholesterol of raising product, serum triglyceride on average descends and reaches more than 15%, shows to have the auxiliary antilipemic effect.
By the test comparative measurements, the hypotensive plant extracts of reducing blood lipid of the present invention does not have influence to the heart rate of spontaneous hypertension rat, and the blood pressure and the heart rate of normal rat do not had influence.Feed spontaneous hypertension rat every day the test group blood pressure of raising product and on average reduce than control group and reach more than the 10mmHg, show that it has the effect of aided blood pressure-lowering.
Above-mentioned evidence, the auxiliary antilipemic that the extract tool of chrysanthemum rice of the present invention and/or chrysanthemum is good, hypotensive effect can be prepared into a kind of high-quality functional food.
Beneficial effect of the present invention is mainly reflected in: providing a kind of is feedstock production with chrysanthemum rice and chrysanthemum, wherein functional component total contents such as galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and chlorogenic acid have the plant extracts of auxiliary antilipemic and hypotensive double effects more than 50%.And the lower chrysanthemum rice of functional component total contents such as galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and chlorogenic acid and the extract result of the test of chrysanthemum do not have auxiliary antilipemic and blood pressure reduction effect.This extract compares with domestic and international existing product, and effect more comprehensively, and is better to demand crowd's recovery normal lipid, blood pressure effect.
(4) specific embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
Get chrysanthemum rice (Suichang County, Zhejiang Province product) 8000 and restrain the pulverizing of milling, add the ethanol water of 32 kilograms of volumetric concentration 80% volumetric concentrations, extracted 2 hours 80 ℃ of insulations, filter extract, the extract vacuum reclaims ethanol, and being concentrated into relative density is about 1.15, gets paste 1856 grams.Get macroporous absorbent resin (model is AB-8, Tianjin Nankai Hecheng S﹠T Co., Ltd. produce),, and wash three times, drain away the water, get 320 liters of wet resins dress posts with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours.With distilled water submergence resin, add paste, with 640 liters of distilled water drip washing, discard leacheate, with the ethanol water drip washing of 320 liter of 20% volumetric concentration, leacheate discards after ethanol is reclaimed in vacuum distillation.Carry out wash-out with 1280 liters of drip washing of volumetric concentration 80% ethanol water again, collect eluent, rate of flow in rinse is 4L/min, whole eluent vacuum of collecting reclaim ethanol for following 50 ℃, and removal moisture, it is moisture below 12% that 50 ℃ in vacuum is dried to, and obtains galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and eight kinds of material total contents of chlorogenic acid and be 53.6% extract 465g.
Embodiment 2:
Get chrysanthemum rice (Suichang County, Zhejiang Province product) 3000 grams, the pulverizing of milling adds the ethanol water of 6021 kilograms of volumetric concentration 50% volumetric concentrations, extracts 3 hours 60 ℃ of insulations, filter extract, extract is added 6.7 liters of 95% ethanol, stirs, static 2 hours, filter once more, the filtrate vacuum reclaims ethanol, and being concentrated into relative density is about 1.10, gets paste 583 grams.Get macroporous absorbent resin (model is AB-8, Tianjin Nankai Hecheng S﹠T Co., Ltd. produce),, and wash three times, drain away the water, get 420 liters of wet resins dress posts with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours.With distilled water submergence resin, add paste, with 840 liters of distilled water drip washing, discard leacheate, with the ethanol water drip washing of 420 liter of 10% volumetric concentration, leacheate discards after ethanol is reclaimed in vacuum distillation.Again with 820 liters of drip washing of volumetric concentration 60% ethanol water, collect eluent, rate of flow in rinse is 1.5L/min, whole eluent vacuum of collecting reclaim ethanol for following 50 ℃, and removal moisture, it is moisture below 12% that 50 ℃ in vacuum is dried to, and obtains galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and eight kinds of material total contents of chlorogenic acid and be 51.3% extract 187g.
Embodiment 3:
Get chrysanthemum rice (Suichang County, Zhejiang Province product) 6000 grams, the pulverizing of milling adds the ethanol water of 33 kilograms of volumetric concentration 70% volumetric concentrations, extracts 1 hour 100 ℃ of insulations, filter extract, extract is added 3.5 liters of 95% ethanol, stirs, static 0.5 hour, filter once more, the filtrate vacuum reclaims ethanol, and being concentrated into relative density is about 1.15, gets paste 1183 grams.Get macroporous absorbent resin (model is AB-8, Tianjin Nankai Hecheng S﹠T Co., Ltd. produce),, and wash three times, drain away the water, get 600 liters of wet resins dress posts with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours.With distilled water submergence resin, add paste, with 1200 liters of distilled water drip washing, discard leacheate, with the ethanol water drip washing of 600 liter of 15% volumetric concentration, leacheate discards after ethanol is reclaimed in vacuum distillation.Again with 650 liters of drip washing of volumetric concentration 90% ethanol water, collect eluent, rate of flow in rinse is 2L/min, whole eluent vacuum of collecting reclaim ethanol for following 50 ℃, and removal moisture, it is moisture below 12% that 50 ℃ in vacuum is dried to, and obtains galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and eight kinds of material total contents of chlorogenic acid and be 52.7% extract 348g.
Get chrysanthemum (Tongxiang, Zhejiang Province city product) 6000 grams, the pulverizing of milling adds the ethanol water of 36 kilograms of volumetric concentration 70% volumetric concentrations, extracts 1 hour 100 ℃ of insulations, filter extract, extract is added 3.8 liters of 95% ethanol, stirs, static 0.5 hour, filter once more, the filtrate vacuum reclaims ethanol, and being concentrated into relative density is about 1.10, gets paste 1312 grams.Get macroporous absorbent resin (model is AB-8, Tianjin Nankai Hecheng S﹠T Co., Ltd. produce),, and wash three times, drain away the water, get 360 liters of wet resins dress posts with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours.With distilled water submergence resin, add paste, with 720 liters of distilled water drip washing, discard leacheate, with the ethanol water drip washing of 360 liter of 15% volumetric concentration, leacheate discards after ethanol is reclaimed in vacuum distillation.Again with 1440 liters of drip washing of volumetric concentration 90% ethanol water, collect eluent, rate of flow in rinse is 3L/min, whole eluent vacuum of collecting reclaim ethanol for following 50 ℃, and removal moisture, it is moisture below 12% that 50 ℃ in vacuum is dried to, and obtains galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and eight kinds of material total contents of chlorogenic acid and be 52.4% extract 368g.
Merge above-mentioned extracted twice thing, get plant extracts 716g.
Embodiment 4:
Get chrysanthemum (Tongxiang, Zhejiang Province city product) 4000 gram, the pulverizing of milling adds the ethanol water of 16 kilograms of volumetric concentration 80% volumetric concentrations, extracted 2 hours 80 ℃ of insulations, filter extract, the extract vacuum reclaims ethanol, being concentrated into relative density is about 1.10, gets paste 746 grams.Get macroporous absorbent resin (model is AB-8, Tianjin Nankai Hecheng S﹠T Co., Ltd. produce),, and wash three times, drain away the water, get 180 liters of wet resins dress posts with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours.With distilled water submergence resin, add paste, with 360 liters of distilled water drip washing, discard leacheate, with the ethanol water drip washing of 180 liter of 20% volumetric concentration, leacheate discards after ethanol is reclaimed in vacuum distillation.Again with 810 liters of drip washing of volumetric concentration 80% ethanol water, collect eluent, rate of flow in rinse is 1.5L/min, whole eluent vacuum of collecting reclaim ethanol for following 50 ℃, and removal moisture, it is moisture below 12% that 50 ℃ in vacuum is dried to, and obtains galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and eight kinds of material total contents of chlorogenic acid and be 54.5% extract 233g.
Embodiment 5:
Get chrysanthemum (Tongxiang, Zhejiang Province city product) 7000 grams, the pulverizing of milling adds the ethanol water of 49 kilograms of volumetric concentration 50% volumetric concentrations, extracts 3 hours 60 ℃ of insulations, filter extract, extract is added 15.5 liters of 95% ethanol, stirs, static 2 hours, filter once more, the filtrate vacuum reclaims ethanol, and being concentrated into relative density is about 1.10, gets paste 1423 grams.Get macroporous absorbent resin (model is AB-8, Tianjin Nankai Hecheng S﹠T Co., Ltd. produce),, and wash three times, drain away the water, get 240 liters of wet resins dress posts with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours.With distilled water submergence resin, add paste, with 480 liters of distilled water drip washing, discard leacheate, with the ethanol water drip washing of 240 liter of 10% volumetric concentration, leacheate discards after ethanol is reclaimed in vacuum distillation.Again with 1080 liters of drip washing of volumetric concentration 60% ethanol water, collect eluent, rate of flow in rinse is 4L/min, whole eluent vacuum of collecting reclaim ethanol for following 50 ℃, and removal moisture, it is moisture below 12% that 50 ℃ in vacuum is dried to, and obtains galuteolin, apiin, cyanidenon, apiolin, robinin glucoside, acacipetalin, flavonoid glycoside and eight kinds of material total contents of chlorogenic acid and be 51.6% extract 437g.
Embodiment 6: auxiliary antilipemic, blood pressure reduction effect test
Get 40 of the healthy adult male rats (purchase of Zhejiang Academy of Medical Sciences animal center) of 150～200g body weight, divide four groups, 10 every group, one group is the high fat of blood control group, and three groups is test group.Four groups of rats all feed raises the feed 7～10 days that contains mass fraction 1% cholesterol, 10% yolk powder, 10% lard, and its serum total cholesterol value is all reached more than the 7.0mmol/L, and serum triglyceride all reaches more than the 2.0mmol/L.Three groups of rats of test, first group of per os irritated stomach, feed every day and raise 5 gram embodiment, 1 extract, second group of per os irritated stomach, feeds every day and raises 3 gram embodiment, 1 extract, the 3rd group of per os irritated stomach, feed every day and raise 4 gram embodiment, 1 extract, high fat of blood group per os is irritated stomach, feeds every day and raises 5 gram water, four groups of rats all continue to feed to be raised and preceding identical feed, tests altogether 30 days.After measured, compare with control group, feed every day and raise 5 gram embodiment, 1 extract group rat blood serum T-CHOL decline average out to 18.6%, serum triglyceride decline average out to 22.3%, feed every day and raise 4 gram embodiment, 1 extract group rat blood serum T-CHOL decline average out to 14.8%, serum triglyceride decline average out to 19.2%, feed every day and raise 3 gram embodiment, 1 extract group rat blood serum T-CHOL decline average out to 11.0%, serum triglyceride decline average out to 16.7%, good auxiliary antilipemic effect is arranged.
Take from and send out 40 of Hypertensive Rats (purchase of Zhejiang Academy of Medical Sciences animal center), male and female half and half, body weight 180～220 grams, measuring blood pressure and heart rate divide four groups, 10 every group.One group is control group, and three groups is test group.First test group per os every day filling stomach is fed and is raised embodiment 1 extract 6 grams, second test group per os every day is irritated stomach and is fed and raise embodiment 1 extract 4 grams, feeds for the 3rd group and raises embodiment 1 extract 5 grams, and control group is fed and raised 6 gram water, four groups of rats all feed normal diet, long run test 30 days simultaneously.Duration of test is twice of measuring blood pressure, heart rate weekly.Off-test, measuring blood pressure and heart rate are 3～5 times in 10 days.
Get 30～40 of normal rats (purchase of Zhejiang Academy of Medical Sciences animal center), requirement same as described above divides three groups, per os filling stomach is fed and is raised product, feeding three groups of every days and raising embodiment 1 extract amount still is one group of one group of 6 gram, one group of 5 gram and 4 gram, and feeds and raise identical feed, continuous 30 days.
By the comparative measurements result, product does not have influence to the heart rate of spontaneous hypertension rat, and the blood pressure and the heart rate of normal rat do not had influence.Feed spontaneous hypertension rat every day and raise 6 gram embodiment, 1 extract group blood pressure and on average reduce 23.4mmHg than control group, feed every day and raise 5 gram embodiment, 1 extract group blood pressure and on average reduce 18.7mmHg, feed every day and raise 4 gram embodiment, 1 extract group blood pressure and on average reduce 16.2mmHg than control group than control group.The effect of aided blood pressure-lowering is preferably arranged.
Above-mentioned evidence, the auxiliary antilipemic that extract tool of the present invention is good, hypotensive effect, this extract can be made have auxiliary antilipemic, the functional food of hypotensive double effects.
Embodiment 7: auxiliary antilipemic, blood pressure reduction effect test.
Get 40 of the healthy adult male rats (purchase of Zhejiang Academy of Medical Sciences animal center) of 150～200g body weight, divide four groups, 10 every group, one group is the high fat of blood control group, and three groups is test group.Four groups of rats all feed raises the feed 7～10 days that contains mass fraction 1% cholesterol, 10% yolk powder, 10% lard, and its serum total cholesterol value is all reached more than the 7.0mmol/L, and serum triglyceride all reaches more than the 2.0mmol/L.Three groups of rats of test, first group of per os irritated stomach, feed every day and raise 5 gram embodiment, 3 extracts, second group of per os irritated stomach, feeds every day and raises 3 gram embodiment, 3 extracts, the 3rd group of per os irritated stomach, feed every day and raise 4 gram embodiment, 3 extracts, high fat of blood group per os is irritated stomach, feeds every day and raises 5 gram water, four groups of rats all continue to feed to be raised and preceding identical feed, tests altogether 30 days.After measured, compare with control group, feed every day and raise 5 gram embodiment, 3 extract group rat blood serum T-CHOL decline average out to 17.3%, serum triglyceride decline average out to 20.9%, feed every day and raise 4 gram embodiment, 3 extract group rat blood serum T-CHOL decline average out to 13.5%, serum triglyceride decline average out to 17.7%, feed every day and raise 3 gram embodiment, 3 extract group rat blood serum T-CHOL decline average out to 10.8%, serum triglyceride decline average out to 16.1%, good auxiliary antilipemic effect is arranged.
Take from and send out 40 of Hypertensive Rats (purchase of Zhejiang Academy of Medical Sciences animal center), male and female half and half, body weight 180-220 gram, measuring blood pressure and heart rate divide four groups, 10 every group.One group is control group, and three groups is test group.First test group per os every day filling stomach is fed and is raised embodiment 3 extracts 6 grams, second test group per os every day is irritated stomach and is fed and raise embodiment 3 extracts 4 grams, feeds for the 3rd group and raises embodiment 3 extracts 5 grams, and control group is fed and raised 6 gram water, four groups of rats all feed normal diet, long run test 30 days simultaneously.Duration of test is twice of measuring blood pressure, heart rate weekly.Off-test, measuring blood pressure and heart rate are 3-5 time in 10 days.
Get normal rat 30-40 only (purchase of Zhejiang Academy of Medical Sciences animal center), requirement same as described above divides three groups, per os filling stomach is fed and is raised product, feeding three groups of every days and raising embodiment 3 extract amounts still is one group of one group of 6 gram, one group of 5 gram and 4 gram, and feeds and raise identical feed, continuous 30 days.
By the comparative measurements result, product does not have influence to the heart rate of spontaneous hypertension rat, and the blood pressure and the heart rate of normal rat do not had influence.Feed spontaneous hypertension rat every day and raise 6 gram embodiment, 3 extract group blood pressures and on average reduce 22.5mmHg than control group, feed every day and raise 5 gram embodiment, 3 extract group blood pressures and on average reduce 19.6mmHg, feed every day and raise 4 gram embodiment, 3 extract group blood pressures and on average reduce 17.3mmHg than control group than control group.The effect that good aided blood pressure-lowering is arranged.
Above-mentioned evidence, the auxiliary antilipemic that extract tool of the present invention is good, hypotensive effect, this extract can be made have auxiliary antilipemic, the functional food of hypotensive double effects.
Embodiment 8: auxiliary antilipemic, blood pressure reduction effect test.
Get 40 of the healthy adult male rats (purchase of Zhejiang Academy of Medical Sciences animal center) of 150～200g body weight, divide four groups, 10 every group, one group is the high fat of blood control group, and three groups is test group.Four groups of rats all feed raises the feed 7～10 days that contains mass fraction 1% cholesterol, 10% yolk powder, 10% lard, and its serum total cholesterol value is all reached more than the 7.0mmol/L, and serum triglyceride all reaches more than the 2.0mmol/L.Three groups of rats of test, first group of per os irritated stomach, feed every day and raise 5 gram embodiment, 5 extracts, second group of per os irritated stomach, feeds every day and raises 3 gram embodiment, 5 extracts, the 3rd group of per os irritated stomach, feed every day and raise 4 gram embodiment, 5 extracts, high fat of blood group per os is irritated stomach, feeds every day and raises 5 gram water, four groups of rats all continue to feed to be raised and preceding identical feed, tests altogether 30 days.After measured, compare with control group, feed every day and raise 5 gram embodiment, 3 extract group rat blood serum T-CHOL decline average out to 18.4%, serum triglyceride decline average out to 20.7%, feed every day and raise 4 gram embodiment, 3 extract group rat blood serum T-CHOL decline average out to 14.1%, serum triglyceride decline average out to 16.9%, feed every day and raise 3 gram embodiment, 3 extract group rat blood serum T-CHOL decline average out to 10.3%, serum triglyceride decline average out to 16.6%, good auxiliary antilipemic effect is arranged.
Take from and send out 40 of Hypertensive Rats (purchase of Zhejiang Academy of Medical Sciences animal center), male and female half and half, body weight 180-220 gram, measuring blood pressure and heart rate divide four groups, 10 every group.One group is control group, and three groups is test group.First test group per os every day filling stomach is fed and is raised embodiment 5 extracts 6 grams, second test group per os every day is irritated stomach and is fed and raise embodiment 5 extracts 4 grams, feeds for the 3rd group and raises embodiment 5 extracts 5 grams, and control group is fed and raised 6 gram water, four groups of rats all feed normal diet, long run test 30 days simultaneously.Duration of test is twice of measuring blood pressure, heart rate weekly.Off-test, measuring blood pressure and heart rate are 3-5 time in 10 days.
Get normal rat 30-40 only (purchase of Zhejiang Academy of Medical Sciences animal center), requirement same as described above divides three groups, per os filling stomach is fed and is raised product, feeding three groups of every days and raising embodiment 5 extract amounts still is one group of one group of 6 gram, one group of 5 gram and 4 gram, and feeds and raise identical feed, continuous 30 days.
By the comparative measurements result, product does not have influence to the heart rate of spontaneous hypertension rat, and the blood pressure and the heart rate of normal rat do not had influence.Feed spontaneous hypertension rat every day and raise 6 gram embodiment, 5 extract group blood pressures and on average reduce 19.7mmHg than control group, feed every day and raise 5 gram embodiment, 3 extract group blood pressures and on average reduce 16.5mmHg, feed every day and raise 4 gram embodiment, 3 extract group blood pressures and on average reduce 14.8mmHg than control group than control group.The effect that good aided blood pressure-lowering is arranged.
Above-mentioned evidence, the auxiliary antilipemic that extract tool of the present invention is good, hypotensive effect, this extract can be made have auxiliary antilipemic, the functional food of hypotensive double effects.
Claims (5)
1. hypotensive plant extracts of reducing blood lipid, obtained by following method: the mixture with chrysanthemum or chrysanthemum rice or chrysanthemum and chrysanthemum rice is a raw material, pulverizes, and adds the ethanol water of 50～80% volumetric concentrations of 4～7 times of quality, 60～100 ℃ of insulations were extracted 1～3 hour, filtration obtains extract, adjusts ethanol volumetric concentration to 75%～80% in the extract, stirs, left standstill 0.5～2 hour, filter once more, get filtrate recycling ethanol, concentrate paste; Macroporous absorbent resin post on the paste, distilled water drip washing, again with the ethanol water drip washing of 10～20% volumetric concentrations, remove impurity, last ethanol water wash-out with 60～90% volumetric concentrations, collect eluent and reclaim ethanol, be dried to water content below 12%, get the hypotensive plant extracts of described reducing blood lipid.
2. the method for preparing the hypotensive plant extracts of reducing blood lipid as claimed in claim 1, described method comprises: the mixture with chrysanthemum or chrysanthemum rice or chrysanthemum and chrysanthemum rice is a raw material, pulverizes, and adds the ethanol water of 50～80% volumetric concentrations of 4～7 times of quality, 60～100 ℃ of insulations were extracted 1～3 hour, filtration obtains extract, adjusts ethanol volumetric concentration to 75%～80% in the extract, stirs, left standstill 0.5～2 hour, filter once more, get filtrate recycling ethanol, concentrate paste; Macroporous absorbent resin post on the paste, elder generation's distilled water drip washing, remove impurity with the ethanol water drip washing of 10～20% volumetric concentrations again, last ethanol water wash-out with 60～90% volumetric concentrations, collect eluent and reclaim ethanol, be dried to water content below 12%, get the hypotensive plant extracts of described reducing blood lipid.
3. method as claimed in claim 2 is characterized in that: the ethanol water volumetric usage of described 60～90% volumetric concentrations is 3～5 times of macroporous absorbent resin volume, elution flow rate 1.5～4L/min.
4. method as claimed in claim 2 is characterized in that described method is as follows: the mixture with chrysanthemum or chrysanthemum rice or chrysanthemum and chrysanthemum rice is a raw material, pulverizes, the ethanol water that adds 50～70% volumetric concentrations of 4～7 times of quality, 60～100 ℃ of insulations were extracted 1～3 hour, filtered and obtained extract, regulated ethanol to ethanol volumetric concentration 75%～80%, stir, left standstill 0.5～2 hour, and filtered once more, get filtrate recycling ethanol, being concentrated into relative density is 1.10～1.15, gets paste; Get macroporous absorbent resin with soaked in absolute ethyl alcohol 24 hours, distilled water immersion 24 hours, again with distilled water washing three times, drain, get the amount dress post of 1L macroporous absorbent resin with 2.0～4.0g medicinal extract thing, macroporous absorbent resin post on the paste, elder generation is with the distilled water drip washing of 2 times of volumes of macroporous absorbent resin, remove impurity with the ethanol water drip washing of 10～20% volumetric concentrations of 1 times of volume of macroporous absorbent resin again, at last with the ethanol water wash-out of 60～90% volumetric concentrations of 3.5～4.5 times of volumes of macroporous absorbent resin, collect the eluent vacuum and reclaim ethanol for 50 ℃, be dried to water content below 12%, get the hypotensive plant extracts of described reducing blood lipid.
5. the hypotensive plant extracts of reducing blood lipid as claimed in claim 1 is used in the functional food of preparation tool auxiliary antilipemic, hypotensive double effects.
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| CNA2008100609226A CN101248870A (en) | 2008-04-02 | 2008-04-02 | Lowering blood and blood pressure plants extract, preparation and applications thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101972291A (en) * | 2010-09-30 | 2011-02-16 | 哈尔滨医科大学 | Helichrysum arenarium Moench extractive as well as preparation method and application thereof |
| CN102491964A (en) * | 2011-12-11 | 2012-06-13 | 卞毓平 | Method for extracting acacetin from chrysanthemum |
| CN102772466A (en) * | 2012-08-29 | 2012-11-14 | 苏州大学 | Application of chrysanthemum extract |
| CN104107206A (en) * | 2014-06-13 | 2014-10-22 | 苏州大学 | Application of Hangzhou chrysanthemum extract product in preparation of healthcare foods and medicines for preventing and treating hyperglycemia |
| US12128082B2 (en) * | 2021-12-23 | 2024-10-29 | Industrial Technology Research Institute | Method for extracting flavone aglycones in chrysanthemum morifolium, extract obtained thereby and anti-inflammatory pharmaceutical composition |
-
2008
- 2008-04-02 CN CNA2008100609226A patent/CN101248870A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101972291A (en) * | 2010-09-30 | 2011-02-16 | 哈尔滨医科大学 | Helichrysum arenarium Moench extractive as well as preparation method and application thereof |
| CN101972291B (en) * | 2010-09-30 | 2012-11-21 | 哈尔滨医科大学 | Helichrysum arenarium Moench extractive as well as preparation method and application thereof |
| CN102491964A (en) * | 2011-12-11 | 2012-06-13 | 卞毓平 | Method for extracting acacetin from chrysanthemum |
| CN102491964B (en) * | 2011-12-11 | 2013-08-21 | 湖北诺克特药业有限公司 | Method for extracting acacetin from chrysanthemum |
| CN102772466A (en) * | 2012-08-29 | 2012-11-14 | 苏州大学 | Application of chrysanthemum extract |
| CN104107206A (en) * | 2014-06-13 | 2014-10-22 | 苏州大学 | Application of Hangzhou chrysanthemum extract product in preparation of healthcare foods and medicines for preventing and treating hyperglycemia |
| US12128082B2 (en) * | 2021-12-23 | 2024-10-29 | Industrial Technology Research Institute | Method for extracting flavone aglycones in chrysanthemum morifolium, extract obtained thereby and anti-inflammatory pharmaceutical composition |
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