CN101164880A - Method for guiding chiral silicon oxide synthesis by DNA template - Google Patents
Method for guiding chiral silicon oxide synthesis by DNA template Download PDFInfo
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- CN101164880A CN101164880A CNA2007100592678A CN200710059267A CN101164880A CN 101164880 A CN101164880 A CN 101164880A CN A2007100592678 A CNA2007100592678 A CN A2007100592678A CN 200710059267 A CN200710059267 A CN 200710059267A CN 101164880 A CN101164880 A CN 101164880A
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Abstract
This invention discloses a method for synthesizing chiral silicon oxide guided by DNA matrix. The steps are: ethanol or methanol is mixed with the predecessor of silicon source to obtain the even solution; (2) DNA series is dissolved in NaCl solution, rising its temperature and then lowering the temperature, and being mild cultivation at 4deg.C for 4-24hrs; (3), the solution from step1 is added with the DNA solution from step 2, agitating at 3-40deg.C for 3-48hrs to obtain the inventive product. In this invention, utilized is the assembling character of the DNA moleculae and the interaction of the predecessor with said moleculae for guiding the hydrolysis products of the predecessor growth in proper to produce micron grade sized and chiral structure inorganic materials. This inventive method has the potencial value for application of chiral separation and asymmetrical catalysis.
Description
Technical field
The invention belongs to technical field, relate to a kind of method with dna profiling guiding synthesis of chiral inorganic materials with chemical self-assembling method manufacturing function material.
Background technology
Occurring in nature chiral inorganic material is very rare, and emerging molecular engineering---with the function is guiding, by computer aided design (CAD), the orientation of research specified property material synthetic with assembling---for chiral inorganic material synthetic opened up the new visual field.For example, Xu Ruren academician seminar is that template has been synthesized the zinc phosphate with foraminous spiral tract with chirality organo-metallic mixture
[1]The chiral liquid crystal that employing mnyristoyl-L-Ala salt such as Shunal Che and quaternized aminosilane are formed is template mutually, and it is the chirality mesoporous silica material of 2.2nm that channel diameter has been synthesized in guiding
[2]These achievements in research have indicated that template has crucial effects to inorganic materials synthetic.
The important substance of storage genetic information in the organism---thymus nucleic acid (DNA) is because of having highly narrow spectrum molecular recognition effect, and is easy to characteristics such as chemosynthesis, structure polymorphism, becomes the focus of function field of new research recently
[3]For example, Warner and Hutchison adopt the dna molecular skeleton, and the guiding nm gold particles has formed line style, branching type and ribbon conductor structure
[4]Numata etc. are that template has been synthesized the shaft-like of submicron-scale and ring-type silicon oxide particle with colibacillary double-stranded DNA (diameter is 2nm)
[5]Along with further investigation, study report about the assembling of three chains, four chains or multichain DNA and more increase the dna structure polymorphism.For example, Batalia
[6]The radial width that forms Deng the DNA multichain assembling by being rich in guanine base (G) of having reported a kind of novelty is the lead-type structure that 2.1nm and 6.7nm alternately change in order.The DNA multichain assembly that these emerge in an endless stream is for the development of functional materials provides multifarious formwork structure.On the other hand, DNA is the biomacromolecule of " chirality " information of a kind of containing, and can produce chiral induction effect---Garoff etc.
[7]Confirmed that achiral cyanine dye molecule can spontaneous formation dextrorotation aggregate under the effect of duplex DNA.These novel results of study show that regulating DNA supramolecule packaging assembly has the desirable template of chirality and ad hoc structure with design, and then guiding chirality or complicated texture inorganic materials synthetic research thinking is practicable.
[1]Y.Wang，J.H.Yu，M.Guo，R.R.Xu.“[{Zn
2(HPO
4)
4}{Co(dien)
2}]H
3O：A?zinc?phosphate?withmultidirectional?intersecting?helical?channels”Angew.Chem.Int.Ed.42(2003)，4089-4092
[2]S.Che，Z.Liu，T.Ohsuna，K.Sakamoto，O.Terasaki?and?T.Tatsumi.“Synthesis?and?characterization?of?chiralmesoporous?silca”，Nature?429(2004)281-284
[3]P.Albert?and?J.L.Mergny，“DNA?duplex-quadruplex?exchange?as?the?basis?for?a?nanomolecular?machine”，Proc.Natl.4cad.Sci.USA，2003，100，1569-1573
[4]M.G.Warner?and?J.E.Hutchison，“Linear?assemblies?of?nanoparticles?electrostatically?organized?on?DNAscaffolds”Nature?Materials?2(2003)272-278
[5]M.Numata，K.Sugiyasu，T.Hasegawa?and?S.Shinkai“Sol-Gel?reaction?using?DNA?as?a?template：an?attempttoward?transcription?of?DNA?into?inorganic?materials”Angew.Chem.Int.Ed.43(2004)3279-3283
[6]M.Batalia，E.Protozanova，R.B.Macgregor?Jr.and?D.Erie，“Self-assembly?of?Frayed?wires?and?Frayed?wirenetworks：nanoconstruction?with?multistranded?DNA”Nano?Letters?2(2002)269-274
[7]R.A.Garoff，E.A.Litzinger，R.E.Connor，I.Fisherman，and?B.A.Armitage“Helical?aggregation?of?cyaninedyes?on?DNA?templates：effect?of?dye?structure?on?formation?of?homo-and?heteroaggregates”Langmuir?18(2002)6330-6337
Summary of the invention
The purpose of this invention is to provide a kind of method with the dna profiling guiding chiral silicon oxide synthesis.
Technical scheme of the present invention is summarized as follows:
A kind of method with the dna profiling guiding chiral silicon oxide synthesis, form by following step:
(1) be 5-20 with volume ratio: 1 ethanol or methyl alcohol, silicon source presoma mix, and at 10-50 ℃, react 0.5-18h, obtain the homogeneous solution of siliceous source presoma;
(2) dna sequence dna is dissolved in the 0.02-0.3M NaCl aqueous solution in room temperature, the ultimate density that makes DNA is 1-100 μ M, and solution is warming up to 80-95 ℃, reduces to 20-30 ℃ again, and at 4 ℃ of incubation 4-24h;
(3) be 40-80 by volume in the dna solution that step (2) makes: 1 ratio adds the solution that step (1) makes, and stirs, and at 3-40 ℃ of reaction 3-48h, makes a kind of with dna profiling guiding synthetic chiral silicon oxide.
Described silicon source presoma is methyl silicate or tetraethoxy or positive isopropyl silicate or aminopropyl trimethoxysilane or aminopropyl triethoxysilane.
Described dna sequence dna is GxTyGz, and described G is guanine deoxyribonucleoside acid, and described T is a thymidylic acid, and described x is 2-8, and y is 3-6, and z is 2-8.
Characteristics of the present invention be to utilize dna molecular assembling characteristic and and silicon source precursor molecule between weak interaction force, the hydrolysate ordering growth of guiding silicon source presoma generates the micron order yardstick, has the inorganic materials of chiral structure feature.The made chiral inorganic material of the present invention has the potential using value at aspects such as chiral separation, asymmetry catalysis.
Description of drawings
Fig. 1 is atomic force microscope (AFM) figure with a kind of chiral silicon oxide of method synthetic of the present invention.
Fig. 2 is the AFM figure with the another kind of chiral silicon oxide of method synthetic of the present invention.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
The preparation method of dna sequence dna does not limit, can be synthetic with automatic DNA synthesizer DNA or phosphoramidic acid method.
Embodiment 1
A kind of method with the dna profiling guiding chiral silicon oxide synthesis, form by following step:
(1) be that 10: 1 methyl alcohol, aminopropyl trimethoxysilane mixes and follows vigorous stirring simultaneously with volume ratio, at 30 ℃, reaction 8h obtains the homogeneous solution of siliceous source presoma;
(2) dna sequence dna is dissolved in the 0.02M NaCl aqueous solution in room temperature, the ultimate density that makes DNA is 1 μ M, and solution is warming up to 90 ℃, reduce to 25 ℃ (this process is the intensification annealing process) again, and at 4 ℃ of incubation 20h, described dna sequence dna is G
2T
3G
4, described G is guanine deoxyribonucleoside acid, T is a thymidylic acid;
(3) be that 60: 1 ratio adds the solution that step (1) makes by volume in the dna solution that step (2) makes, stir,, make a kind of with dna profiling guiding synthetic chiral silicon oxide at 25 ℃ of reaction 20h.
Embodiment 1 a kind of of preparation sees Fig. 1 with the dna profiling guiding chiral silicon oxide synthesis.When having DNA, add the AFM figure (sweep limit 10 μ m*10 μ m) of the chiral silicon oxide of silicon source presoma aminopropyl trimethoxysilane solution reaction certain hour formation.
Embodiment 2
A kind of method with the dna profiling guiding chiral silicon oxide synthesis, form by following step:
(1) be that 15: 1 ethanol, aminopropyl triethoxysilane mixes and follows vigorous stirring simultaneously with volume ratio, at 40 ℃, reaction 10h obtains the homogeneous solution of siliceous source presoma;
(2) dna sequence dna is dissolved in the 0.3M NaCl aqueous solution in room temperature, the ultimate density that makes DNA is 1 μ M, and solution is warming up to 95 ℃, reduces to 30 ℃ again, and at 4 ℃ of incubation 24h, described dna sequence dna is G8T
6G
8, described G is guanine deoxyribonucleoside acid, T is a thymidylic acid;
(3) be that 70: 1 ratio adds the solution that step (1) makes by volume in the dna solution that step (2) makes, stir,, make a kind of with dna profiling guiding synthetic chiral silicon oxide at 30 ℃ of reaction 15h.
Embodiment 2 a kind of of preparation see Fig. 2 with the dna profiling guiding chiral silicon oxide synthesis.When having DNA, add the AFM figure (sweep limit 10 μ m*10 μ m) of the chiral silicon oxide of silicon source presoma aminopropyl triethoxysilane solution reaction certain hour formation.
Embodiment 3
A kind of method with the dna profiling guiding chiral silicon oxide synthesis, form by following step:
(1) be that 20: 1 ethanol, tetraethoxy mixes and follows vigorous stirring simultaneously with volume ratio, at 50 ℃, reaction 0.5h obtains the homogeneous solution of siliceous source presoma;
(2) dna sequence dna is dissolved in the 0.3M NaCl aqueous solution in room temperature, the ultimate density that makes DNA is 100 μ M, and solution is warming up to 80 ℃, reduces to 20 ℃ again, and at 4 ℃ of incubation 4h, described dna sequence dna is G
2T
3G
8, described G is guanine deoxyribonucleoside acid, T is a thymidylic acid;
(3) be that 40: 1 ratio adds the solution that step (1) makes by volume in the dna solution that step (2) makes, stir,, make a kind of with dna profiling guiding synthetic chiral silicon oxide at 40 ℃ of reaction 3h.
Embodiment 4
A kind of method with the dna profiling guiding chiral silicon oxide synthesis, form by following step:
(1) be that 5: 1 methyl alcohol, methyl silicate mixes and follows vigorous stirring simultaneously with volume ratio, at 10 ℃, reaction 18h obtains the homogeneous solution of siliceous source presoma;
(2) dna sequence dna is dissolved in the 0.1M NaCl aqueous solution in room temperature, the ultimate density that makes DNA is 10 μ M, and solution is warming up to 90 ℃, reduces to 25 ℃ again, and at 4 ℃ of incubation 12h, described dna sequence dna is G
6T
4G
2, described G is guanine deoxyribonucleoside acid, T is a thymidylic acid;
(3) be that 80: 1 ratio adds the solution that step (1) makes by volume in the dna solution that step (2) makes, stir,, make a kind of with dna profiling guiding synthetic chiral silicon oxide at 3 ℃ of reaction 48h.
Embodiment 5
A kind of method with the dna profiling guiding chiral silicon oxide synthesis, form by following step:
(1) is that vigorous stirring is followed in 10: 1 ethanol, positive isopropyl silicate mixing simultaneously with volume ratio,, reacts 18h, obtain the homogeneous solution of siliceous source presoma at 20 ℃;
(2) dna sequence dna is dissolved in the 0.1M NaCl aqueous solution in room temperature, the ultimate density that makes DNA is 10 μ M, and solution is warming up to 90 ℃, reduces to 25 ℃ again, and at 4 ℃ of incubation 12h, described dna sequence dna is G
4T
5G
6, described G is guanine deoxyribonucleoside acid, T is a thymidylic acid;
(3) be that 80: 1 ratio adds the solution that step (1) makes by volume in the dna solution that step (2) makes, stir,, make a kind of with dna profiling guiding synthetic chiral silicon oxide at 3 ℃ of reaction 48h.
Claims (3)
1. method with the dna profiling guiding chiral silicon oxide synthesis is characterized in that being made up of following step:
(1) be 5-20 with volume ratio: 1 ethanol or methyl alcohol, silicon source presoma mix, and at 10-50 ℃, react 0.5-18h, obtain the homogeneous solution of siliceous source presoma;
(2) dna sequence dna is dissolved in the 0.02-0.3M NaCl aqueous solution in room temperature, the ultimate density that makes DNA is 1-100 μ M, and solution is warming up to 80-95 ℃, reduces to 20-30 ℃ again, and at 4 ℃ of incubation 4-24h;
(3) be 40-80 by volume in the dna solution that step (2) makes: 1 ratio adds the solution that step (1) makes, and stirs, and at 3-40 ℃ of reaction 3-48h, makes a kind of with dna profiling guiding synthetic chiral silicon oxide.
2. a kind of method with the dna profiling guiding chiral silicon oxide synthesis according to claim 1 is characterized in that described silicon source presoma is methyl silicate or tetraethoxy or positive isopropyl silicate or aminopropyl trimethoxysilane or aminopropyl triethoxysilane.
3. a kind of method with the dna profiling guiding chiral silicon oxide synthesis according to claim 1 is characterized in that described dna sequence dna is GxTyGz, and described G is guanine deoxyribonucleoside acid, described T is a thymidylic acid, described x is 2-8, and y is 3-6, and z is 2-8.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103417975A (en) * | 2013-07-12 | 2013-12-04 | 四川大学 | Preparing method of corrosion type silicon dioxide xerogel drug sustained-release material |
| CN106882812A (en) * | 2017-04-24 | 2017-06-23 | 厦门大学 | A kind of preparation method of the Nano particles of silicon dioxide of morphology controllable |
| CN109534349A (en) * | 2018-03-30 | 2019-03-29 | 中国科学院上海应用物理研究所 | A kind of synthetic method and application of organic mineralized structures based on frame nucleic acid encode |
| CN114849643A (en) * | 2022-04-27 | 2022-08-05 | 西安建筑科技大学 | Coal gangue-based porous material taking actinomycetes as template and preparation method thereof |
-
2007
- 2007-08-24 CN CNB2007100592678A patent/CN100560488C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103417975A (en) * | 2013-07-12 | 2013-12-04 | 四川大学 | Preparing method of corrosion type silicon dioxide xerogel drug sustained-release material |
| CN103417975B (en) * | 2013-07-12 | 2016-02-10 | 四川大学 | A kind of preparation method of corrosion type silicon dioxide xerogel drug sustained-release material |
| CN106882812A (en) * | 2017-04-24 | 2017-06-23 | 厦门大学 | A kind of preparation method of the Nano particles of silicon dioxide of morphology controllable |
| CN109534349A (en) * | 2018-03-30 | 2019-03-29 | 中国科学院上海应用物理研究所 | A kind of synthetic method and application of organic mineralized structures based on frame nucleic acid encode |
| CN114849643A (en) * | 2022-04-27 | 2022-08-05 | 西安建筑科技大学 | Coal gangue-based porous material taking actinomycetes as template and preparation method thereof |
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