CN101118226A - Multifunctional micro-electrode chip circulation detecting pool device - Google Patents
Multifunctional micro-electrode chip circulation detecting pool device Download PDFInfo
- Publication number
- CN101118226A CN101118226A CNA2007100707099A CN200710070709A CN101118226A CN 101118226 A CN101118226 A CN 101118226A CN A2007100707099 A CNA2007100707099 A CN A2007100707099A CN 200710070709 A CN200710070709 A CN 200710070709A CN 101118226 A CN101118226 A CN 101118226A
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- electrode chip
- guide rail
- elastic membrane
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- 239000012530 fluid Substances 0.000 claims abstract description 26
- 230000005672 electromagnetic field Effects 0.000 claims abstract description 18
- 239000012528 membrane Substances 0.000 claims description 25
- 239000004020 conductor Substances 0.000 claims description 4
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 2
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 abstract description 3
- 238000009434 installation Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 239000011324 bead Substances 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
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Abstract
The present invention discloses a multifunctional microelectrode chip circulation detecting pool device. The upper end face of a lower pressed plate is embedded with an electromagnetic field coil, and the two sides are provided with guide rail electrodes. The guide rail electrodes are respectively and electrically connected with the coil lead of the electromagnetic field coil. The lower end face of an elastic film is embedded with a microelectrode chip and the upper end face is provided with a circulating groove. The two sides of an upper pressed plate are respectively provided with guide rail electrodes. The guide rail electrodes are respectively and electrically connected with the electrode lead of the microelectrode chip. The upper pressed plate is provided with a fluid inlet and a fluid outlet. A pipeline is arranged on the fluid inlet and the fluid outlet of the upper pressed plate by two pipeline connectors. The microelectrode chip is positioned at the lower part of the elastic film and the upper part of the lower pressed plate. The electromagnetic field coil is positioned at the lower part of the microelectrode chip and the upper part of the lower pressed plate. The fluid inlet and the fluid outlet respectively aim at the two end ports of the circulating groove of the elastic film. The present invention has the advantages of simple structure, advanced function and convenient installation and cleaning. The present invention can be widely used in the fields of biology, chemistry and life sciences.
Description
Technical field
The present invention relates to a kind of multifunctional micro-electrode chip circulation detecting pool device.
Background technology
Micro-fluidic chip can reach micron and nanoscale because its physical size is very little, and the detection characteristic of the excellence that it had is widely used in fields such as biology, chemistry, life sciences.At present, the general foot of micro-fluidic chip is made up of micro-electrode chip and detection cell, by certain technology (chemical bond or physical bond) micro-electrode chip and detection cell fixedly are assembled into a micro-fluidic chip, but the production of micro-fluidic chip and processing but are subject to processing equipment and technology limitation, performance is subjected to very big influence, as problems such as flow cell obstruction, leakage, cleaning difficulties.
Summary of the invention
The purpose of this invention is to provide a kind of multifunctional micro-electrode chip circulation detecting pool device.
The technical solution used in the present invention is as follows:
Comprise top board, elastic membrane, lower platen, micro-electrode chip, electromagnetic field coil, four guide rail electrodes, two pipe jointers and two pipelines.The lower platen upper surface is embedded with the electromagnetic field coil, lower platen two long sides are respectively equipped with the guide rail electrode, two guide rail electrodes are electrically connected with the coil lead of electromagnetic field coil respectively, the elastic membrane lower surface is embedded with micro-electrode chip, the elastic membrane upper surface has circulation groove, top board two long sides are respectively equipped with the guide rail electrode, two guide rail electrodes are electrically connected with the contact conductor of micro-electrode chip respectively, top board has fluid intake and fluid egress point, and two pipe jointers are by being threaded respectively fluid intake and the outlet fluid of piping erection at top board; Top board, lower platen and elastic membrane are fixed by set bolt, wherein micro-electrode chip is positioned at the below of elastic membrane and is positioned at the top of lower platen, the electromagnetic field coil is positioned at the below of micro-electrode chip and is positioned at the top of lower platen, and fluid intake and outlet fluid are aligned in the two-port of elastic membrane circulation groove respectively.
The beneficial effect that the present invention has is: multifunctional micro-electrode chip circulation detecting pool of the present invention, simple in structure, function advanced person, it is convenient to install and clean, the fields such as biology, chemistry, life science that can be used in are extensive use of, can replace complicated micro-fluidic chip based on MEMS (micro electronmechanical system of processing) technology, but all widespread uses in life science, chemical analysis, food security, environmental monitoring and relevant scientific domain.
Description of drawings
Fig. 1 is a system schematic of the present invention;
Fig. 2 is micro-electrode chip figure of the present invention;
Fig. 3 is an elastic membrane vertical view of the present invention;
Fig. 4 is the A-A sectional view of Fig. 3;
Fig. 5 is a top board vertical view of the present invention;
Fig. 6 is the front view of Fig. 5;
Fig. 7 is a lower platen vertical view of the present invention;
Fig. 8 is the A-A sectional view of Fig. 7.
Among the figure, 1, top board, 2, elastic membrane, 3, lower platen, 4, micro-electrode chip, 5, the electromagnetic field coil, 6,7, pipe jointer, 8,9,10,11, the guide rail electrode, 12,13, pipeline, 14,15, set bolt, 101,102,103,104, fixed orifice, 105, fluid intake, 106, fluid egress point, 201, circulation groove, 202,203,204,205, fixed orifice, 301,302,303,304, fixed orifice, 401,402, contact conductor, 501,502, coil lead.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
As Fig. 1～shown in Figure 8, the present invention includes top board 1, elastic membrane 2, lower platen 3, micro-electrode chip 4, electromagnetic field coil 5, four 6,7 and two pipelines 12,13 of 8,9,10,11, two pipe jointers of guide rail electrode; Lower platen 3 upper surfaces are embedded with electromagnetic field coil 5,3 liang of long sides of lower platen are respectively equipped with guide rail electrode 10,11, two guide rail electrodes 10,11 respectively with the coil lead 501 of electromagnetic field coil 5,502 are electrically connected, elastic membrane 2 lower surfaces are embedded with micro-electrode chip 4, elastic membrane 2 upper surfaces have circulation groove 201,1 liang of long side of top board is respectively equipped with guide rail electrode 8,9, two guide rail electrodes 8,9 respectively with the contact conductor 401 of micro-electrode chip 4,402 are electrically connected, top board 1 has fluid intake 105 and fluid egress point 106, two pipe jointers 6,7 by being threaded respectively pipeline 12,13 are installed in the fluid intake 105 and the outlet fluid 106 of top board 1; Top board 1, lower platen 3 and elastic membrane 2 are fixed by set bolt, be the fixed orifice 101,102,103,104 on 1 four jiaos of the top boards, fixed orifice 301,302,303, the 304 usefulness set bolts 14 on 3 four jiaos of fixed orifice 202,203,204,205 on 2 four jiaos of the elastic membranes and the lower platens and 15 fixing.Wherein micro-electrode chip 4 is positioned at the below of elastic membrane 2 and is positioned at the top of lower platen 3, electromagnetic field coil 5 is positioned at the below of micro-electrode chip 4 and is positioned at the top of lower platen 3, and fluid intake 105 and outlet fluid 106 are aligned in the two-port of elastic membrane 2 circulation grooves 201 respectively.
Described micro-electrode chip 4 adopts the micro-electrode chip of American AB TECH company.Described elastic membrane 2 adopts the PDMS material.Angle between described two pipe jointers 6,7 and the normal is 35 degree～85 degree.
Application process of the present invention is as follows:
1, micro-electrode chip circulation detecting pool device is pushed in the existing impedance detection instrument, guide rail electrode 8,9 on the top board 1 is connected with the signal input part of impedance detection instrument respectively, and the guide rail electrode 10,11 on the lower platen 3 is connected with electromagnetic field coil control circuit respectively.
2, sample to be detected is expelled to pipeline 12, goes forward side by side, and flow to the upper surface of micro-electrode chip 4 into circulation groove 201; Circulation groove 201 is vertical with the interdigital electrode of micro-electrode chip 4; The impedance signal of reading the impedance detection instrument just can be known the impedance operator of sample to be detected.
If there is immunity to separate magnetic bead in 3 samples to be detected, start electromagnetic field coil control circuit and produce magnetic field, make immunity separate the surface that magnetic bead accumulates in micro-electrode chip 4, detect the impedance operator that impedance signal just can be known sample to be detected.
4, cleaning electrode only need be dismantled micro-electrode chip circulation detecting pool device, cleans elastic membrane 2 and micro-electrode chip 4 and gets final product, and can reuse chip like this.
5, by changing the size of elastic membrane 2 circulation grooves 201, just can change the size of circulation detecting pool.
Above-mentioned embodiment is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change to the present invention makes all fall into protection scope of the present invention.
Claims (4)
1. multifunctional micro-electrode chip circulation detecting pool device, it is characterized in that: comprise top board (1), elastic membrane (2), lower platen (3), micro-electrode chip (4), electromagnetic field coil (5), four guide rail electrodes (8,9,10,11), two pipe jointers (6,7) and two pipelines (12,13); Lower platen (3) upper surface is embedded with electromagnetic field coil (5), lower platen (3) two long sides are respectively equipped with guide rail electrode (10,11), two guide rail electrodes (10,11) respectively with the coil lead (501 of electromagnetic field coil (5), 502) be electrically connected, elastic membrane (2) lower surface is embedded with micro-electrode chip (4), elastic membrane (2) upper surface has circulation groove (201), top board (1) two long side is respectively equipped with guide rail electrode (8,9), two guide rail electrodes (8,9) respectively with the contact conductor (401 of micro-electrode chip (4), 402) be electrically connected, top board (1) has fluid intake (105) and fluid egress point (106), two pipe jointers (6,7) by being threaded respectively pipeline (12,13) be installed in top board (1) fluid intake (105) and the outlet fluid (106); Top board (1), lower platen (3) and elastic membrane (2) are fixed by set bolt, wherein micro-electrode chip (4) is positioned at the below of elastic membrane (2) and is positioned at the top of lower platen (3), electromagnetic field coil (5) is positioned at the below of micro-electrode chip (4) and is positioned at the top of lower platen (3), and fluid intake (105) and outlet fluid (106) are aligned in the two-port of elastic membrane (2) circulation groove (201) respectively.
2. a kind of multifunctional micro-electrode chip circulation detecting pool device according to claim 1 is characterized in that: described micro-electrode chip (4) adopts the micro-electrode chip of American AB TECH company.
3. a kind of multifunctional micro-electrode chip circulation detecting pool device according to claim 1 is characterized in that: described elastic membrane (2) adopts the PDMS material.
4. a kind of multifunctional micro-electrode chip circulation detecting pool device according to claim 1 is characterized in that: the angle between described two pipe jointers (6,7) and the normal is 35 degree～85 degree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100707099A CN100549683C (en) | 2007-08-06 | 2007-08-06 | Multifunctional micro-electrode chip circulation detecting pool device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100707099A CN100549683C (en) | 2007-08-06 | 2007-08-06 | Multifunctional micro-electrode chip circulation detecting pool device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101118226A true CN101118226A (en) | 2008-02-06 |
| CN100549683C CN100549683C (en) | 2009-10-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007100707099A Expired - Fee Related CN100549683C (en) | 2007-08-06 | 2007-08-06 | Multifunctional micro-electrode chip circulation detecting pool device |
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| CN (1) | CN100549683C (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120061239A1 (en) * | 2010-09-09 | 2012-03-15 | Elibol Oguz H | Electronic and fluidic interface |
| CN102809594A (en) * | 2012-08-18 | 2012-12-05 | 哈尔滨工业大学 | Electrochemistry detection cell |
| CN101788515B (en) * | 2010-01-25 | 2012-12-19 | 首都医科大学附属北京口腔医院 | Method for detecting bacteria by using electrochemical impedance principle and microfluidic chip |
| CN103308559A (en) * | 2013-06-03 | 2013-09-18 | 浙江大学 | Rotating type rapidly-assembled micro-circulation detection pool device |
| CN103323493A (en) * | 2013-06-03 | 2013-09-25 | 浙江大学 | Direct-compression type rapid assembling micro-circulation detection tank device |
| CN103323492A (en) * | 2013-06-03 | 2013-09-25 | 浙江大学 | Rapid assembly micro-flow detection cell device with magnetic separation |
| CN103558135A (en) * | 2013-11-07 | 2014-02-05 | 西南石油大学 | Repeatedly-usable glass chip model |
| CN104777274A (en) * | 2015-04-20 | 2015-07-15 | 浙江大学 | Fixing hold-down device for PDMS micro flow cell |
| CN109239381A (en) * | 2018-08-02 | 2019-01-18 | 深圳大学 | A kind of micro-fluidic chip and micro fluidic device for biomolecule detection |
| CN109331191A (en) * | 2018-09-10 | 2019-02-15 | 浙江大学 | A kind of high voltage electric field coupling ultrasonic wave treatment liquid sterilizing test device and method |
| CN110988087A (en) * | 2019-11-25 | 2020-04-10 | 中国计量大学 | Microfluidic impedance type biological online detection device |
-
2007
- 2007-08-06 CN CNB2007100707099A patent/CN100549683C/en not_active Expired - Fee Related
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101788515B (en) * | 2010-01-25 | 2012-12-19 | 首都医科大学附属北京口腔医院 | Method for detecting bacteria by using electrochemical impedance principle and microfluidic chip |
| US8444835B2 (en) * | 2010-09-09 | 2013-05-21 | Intel Corporation | Electronic and fluidic interface |
| JP2013537803A (en) * | 2010-09-09 | 2013-10-07 | インテル コーポレイション | Electrical fluid interface |
| US20120061239A1 (en) * | 2010-09-09 | 2012-03-15 | Elibol Oguz H | Electronic and fluidic interface |
| CN102809594A (en) * | 2012-08-18 | 2012-12-05 | 哈尔滨工业大学 | Electrochemistry detection cell |
| CN103308559B (en) * | 2013-06-03 | 2015-10-28 | 浙江大学 | The micro-circulation detecting pool device of rotary quick assembling |
| CN103308559A (en) * | 2013-06-03 | 2013-09-18 | 浙江大学 | Rotating type rapidly-assembled micro-circulation detection pool device |
| CN103323493A (en) * | 2013-06-03 | 2013-09-25 | 浙江大学 | Direct-compression type rapid assembling micro-circulation detection tank device |
| CN103323492A (en) * | 2013-06-03 | 2013-09-25 | 浙江大学 | Rapid assembly micro-flow detection cell device with magnetic separation |
| WO2014194556A1 (en) * | 2013-06-03 | 2014-12-11 | 浙江大学 | Direct-pressing quick-assemble microcirculation detection tank device |
| CN103323493B (en) * | 2013-06-03 | 2015-05-06 | 浙江大学 | Direct-compression type rapid assembling micro-circulation detection tank device |
| CN103323492B (en) * | 2013-06-03 | 2015-05-06 | 浙江大学 | Rapid assembly micro-flow detection cell device with magnetic separation |
| CN103558135A (en) * | 2013-11-07 | 2014-02-05 | 西南石油大学 | Repeatedly-usable glass chip model |
| CN104777274A (en) * | 2015-04-20 | 2015-07-15 | 浙江大学 | Fixing hold-down device for PDMS micro flow cell |
| CN109239381A (en) * | 2018-08-02 | 2019-01-18 | 深圳大学 | A kind of micro-fluidic chip and micro fluidic device for biomolecule detection |
| CN109331191A (en) * | 2018-09-10 | 2019-02-15 | 浙江大学 | A kind of high voltage electric field coupling ultrasonic wave treatment liquid sterilizing test device and method |
| CN110988087A (en) * | 2019-11-25 | 2020-04-10 | 中国计量大学 | Microfluidic impedance type biological online detection device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100549683C (en) | 2009-10-14 |
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Granted publication date: 20091014 Termination date: 20120806 |