CN101062260B - Buyin Yishen granule and production method thereof - Google Patents
Buyin Yishen granule and production method thereof Download PDFInfo
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- CN101062260B CN101062260B CN200710054493A CN200710054493A CN101062260B CN 101062260 B CN101062260 B CN 101062260B CN 200710054493 A CN200710054493 A CN 200710054493A CN 200710054493 A CN200710054493 A CN 200710054493A CN 101062260 B CN101062260 B CN 101062260B
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- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 claims description 6
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- 235000019202 steviosides Nutrition 0.000 claims description 4
- 238000000643 oven drying Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 208000019901 Anxiety disease Diseases 0.000 abstract description 12
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- 241000699670 Mus sp. Species 0.000 description 12
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- 238000002474 experimental method Methods 0.000 description 11
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- 229960004756 ethanol Drugs 0.000 description 8
- AAOVKJBEBIDNHE-UHFFFAOYSA-N diazepam Chemical compound N=1CC(=O)N(C)C2=CC=C(Cl)C=C2C=1C1=CC=CC=C1 AAOVKJBEBIDNHE-UHFFFAOYSA-N 0.000 description 7
- 230000007812 deficiency Effects 0.000 description 6
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- LQIAZOCLNBBZQK-UHFFFAOYSA-N 1-(1,2-Diphosphanylethyl)pyrrolidin-2-one Chemical compound PCC(P)N1CCCC1=O LQIAZOCLNBBZQK-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
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- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
本发明涉及一种补阴怡神颗粒及其生产方法，可有效用于防治焦虑的疾病问题，其解决的技术方案是，采用由熟地黄、百合、酸枣仁、知母制成，其生产方法是，先由熟地黄、百合、酸枣仁、知母中制备出水或醇提物，再加入辅料，用乙醇作溶剂制软材，混拌后，过筛，挤出颗粒，烘干成粒即可，本发明组方科学，生产方法先进，产品服用方便，可有效用于防治焦虑症，效果显著，是中药学上的一个创造，并造福于人类。The invention relates to a kind of Buyin Yishen granule and its production method, which can be effectively used to prevent and treat anxiety diseases. Yes, first prepare water or alcohol extracts from rehmannia glutinosa, lily, jujube kernels, and anemarrhena, then add auxiliary materials, use ethanol as a solvent to make soft materials, after mixing, sieve, extrude granules, and dry them into granules. Yes, the present invention has scientific formula, advanced production method, and convenient taking of the product, which can be effectively used to prevent and treat anxiety with remarkable effect, is a creation in Chinese pharmacy, and benefits mankind.
Description
One, technical field
The present invention relates to medical domain, particularly a kind of nourishing YIN soothe the spirit granule and production method thereof.
Two, background technology
Development along with society, the quickening of people's rhythm of life, the raising of special task efficient, corresponding people's the mental status has very big variation, the overdraw situation of health is in continuous increase, the restless phenomenon of spirit happens occasionally, not only influence the healthy of people, and be that work, study etc. bring adverse effect, people begin to note treatment and the control to restless with anxiety disease for this reason, but for various reasons, there is not gratifying novel drugs to take at present as yet, to improve people's health level and quality of life for people.
Three, summary of the invention
At above-mentioned situation, the present invention's purpose just provides a kind of nourishing YIN soothe the spirit granule and production method thereof, can be effective to prevent and treat the disease problem of anxiety, the technical scheme of its solution is, according to the Chinese medicine principle, granule of the present invention adopts by Radix Rehmanniae Preparata, Bulbus Lilii, Semen Ziziphi Spinosae, the Rhizoma Anemarrhenae is made, and its production method is, earlier by Radix Rehmanniae Preparata, Bulbus Lilii, Semen Ziziphi Spinosae, preparation water outlet or ethanol extract in the Rhizoma Anemarrhenae, add adjuvant again, make solvent system soft material, after puddling with ethanol, sieve, extrude granule, the oven dry granulating gets final product prescription science of the present invention, production method advanced person, the product taking convenience can be effective to prevent and treat anxiety neurosis, and effect is remarkable, be a creation on the Chinese materia medica, and bring benefit to the mankind.
Four, the specific embodiment
Below in conjunction with practical situation the specific embodiment of the present invention is elaborated.
According to the aforementioned techniques scheme, granule of the present invention is made by Radix Rehmanniae Preparata 10-20g, Bulbus Lilii 10-15g, Semen Ziziphi Spinosae 15-25g, Rhizoma Anemarrhenae 5-10g, most preferred embodiment is to be made by Radix Rehmanniae Preparata 10g, Bulbus Lilii 12g, Semen Ziziphi Spinosae 20g, Rhizoma Anemarrhenae 6g, in order to guarantee result of use and to take conveniently, also be added with the adjuvant that starch, dextrin and stevioside are formed in the described granule.Said adjuvant by starch, dextrin add again after with weight ratio preparation in 3: 1 starch and dextrin weight and 1/400 stevioside form.The adjuvant addition is ethanol extract or water extract weight and (promptly the ethanol extract of four kinds of Chinese medicines or water extract gross weight are 1: 1 with the adjuvant weight ratio) by Radix Rehmanniae Preparata, Bulbus Lilii, Semen Ziziphi Spinosae, the Rhizoma Anemarrhenae.Starch is the starch that is made by corn or Rhizoma Dioscoreae esculentae.
The nourishing YIN particulate production method of soothing the spirit is, the method that adopts alcohol extraction or water to carry Radix Rehmanniae Preparata, Bulbus Lilii, Semen Ziziphi Spinosae and the Rhizoma Anemarrhenae at first, produce ethanol extract or water extract, can be to extract after four kinds of medicament mixed, also can be to extract respectively, also can be per two kinds of united extraction, the present embodiment method of extracting respectively.
Prepared rehmannia root polysaccharide: Radix Rehmanniae is scrophulariaceae rehmannia glutinosa plant Rehamannia glutinosa Libosch, remove the root of clean earth, fibrous root, rice shoot, steaming becomes Radix Rehmanniae Preparata and dries, be 95% ethanol reflux, extract, 4h, 3h, 3h successively with the mass concentration of 8 times of amounts of Radix Rehmanniae Preparata dry weight, 6 times of amounts, 6 times of amounts respectively again, medicinal residues are put the ventilation and are dried; Medicinal residues add 10 times of amounts of water of its weight respectively, 8 times of amounts, 8 times of amounts, carry 4h in 98～100 ℃ of following water-baths temperature successively, 3h, 3h, solution was considered, merging filtrate, centrifugal 15min, get supernatant, be concentrated into 1: 1 (mg/ml), transfer with 95% ethanol to contain the alcohol amount to 80%, standing over night, sucking filtration gets the crude polysaccharides precipitation, get crude polysaccharides and be dissolved in water, adding equal-volume mass concentration is 30% trichloroacetic acid solution deproteinization, leaves standstill centrifugal, centrifugal liquid is transferred pH7.0 with the sodium hydroxide of 0.01mol/L, concentrating under reduced pressure, the dialysis of concentrated solution flowing water is evaporated to proper volume with dialysis solution, add 95% ethanol accent and contain the alcohol amount to 80%, place, sucking filtration, precipitation is used dehydrated alcohol respectively, acetone, absolute ether washs successively, vacuum drying gets prepared rehmannia root polysaccharide;
Lily aqueous extract: Bulbus Lilii is the dry meat bulb of liliaceous plant tiger lily Lilium brownii lanci folium thumb. or Bulbus Lilii Lilium brownii F.E.Brown var.viridulium Baker or Lilium tenuifolium L.Pumilum DC., decoct with water, the water that adds for the first time 8 times of amounts of Bulbus Lilii weight, boiled 45 minutes, add the water of 6 times of amounts of Bulbus Lilii weight for the second time and for the third time respectively, boiled merging filtrate 30 minutes, concentrating under reduced pressure, vacuum drying gets lily aqueous extract;
Wild jujube kernel alcohol extractive: Semen Ziziphi Spinosae (parched) is the dry mature seed (removing its sarcocarp and outer duricrust) of Rhamnaceae plant Ziziphi Spinosae Ziziphu jujubaMill.Var.spinosae (Bunge) Hu ex HF.Chou, smash to pieces, with the mass concentration of 8 times of amounts of Semen Ziziphi Spinosae weight is 80% alcohol reflux three times, each 1.5h, filter, merge three times filtrate, concentrate and reclaim ethanol to there not being the alcohol flavor, extractum is dissolved in water, with defat with petroleum ether three times, collect lower aqueous layer, water layer is with n-butanol extraction three times, get the upper strata n-butanol layer in 60 ℃ of evaporates to dryness of water-bath, get the dry product wild jujube kernel alcohol extractive;
Rhizoma Anemarrhenae ethanol extract: the Rhizoma Anemarrhenae is a liliaceous plant Rhizoma Anemarrhenae Anemarrhena asphodeloides Bge rhizome, use processed with salt, section, the drying in the sun dry salt Rhizoma Anemarrhenae, 8 times of amounts with Rhizoma Anemarrhenae weight, mass concentration is 80% alcohol reflux three times, each 1.5h, filter, merge three times filtrate, concentrate and reclaim ethanol to there not being the alcohol flavor, extractum adds suitable quantity of water dissolving, with an amount of defat with petroleum ether three times, and the collection lower aqueous layer, water layer is with an amount of n-butanol extraction three times, get the upper strata n-butanol layer and get dry product Rhizoma Anemarrhenae ethanol extract, get prepared rehmannia root polysaccharide 0.3-1g then in 60 ℃ of evaporates to dryness of water-bath, lily aqueous extract 1-3g, wild jujube kernel alcohol extractive 0.2-0.5g, four kinds of raw material blendings of Rhizoma Anemarrhenae ethanol extract 0.2-0.4g, 1: 1 ratio adds adjuvant by weight.After the best is the auxiliary materials and mixing of forming with prepared rehmannia root polysaccharide 0.833g, lily aqueous extract 2.667g, wild jujube kernel alcohol extractive 0.4g, Rhizoma Anemarrhenae ethanol extract 0.333g with by the stevioside of starch 3.17475g, dextrin 1.058258g and 0.01058g, with mass concentration is that 70% ethanol is made soft material as solvent, sieve (being generally 14 mesh sieves), extrusion granulator, put into 60 ℃ of oven dryings 3 hours granule of the present invention, take after the passed examination.
Be noted that; the present invention can be with four kinds of medicines by the component compatibility requirement with Radix Rehmanniae Preparata 10-20g; Bulbus Lilii 10-15g; Semen Ziziphi Spinosae 15-25g; Rhizoma Anemarrhenae 5-10g mixes; directly get ethanol extract or water extract with alcohol or water extracting method; add adjuvant again; make soft material with ethanol as solvent; sieve; extrusion granulator is dried to certified products again; even four kinds of direct decoctions of Chinese medicine; to preventing and treating the anxiety disease certain effect is arranged also; therefore, which kind of form pharmacy that don't work is so long as by Radix Rehmanniae Preparata; Bulbus Lilii; Semen Ziziphi Spinosae; it all is the scope of protection of the invention that four kinds of drug regimens of the Rhizoma Anemarrhenae or extract combination or blended extract are used to prevent and treat anxiety neurosis.
Above-mentioned granule of the present invention (medicine) has the nourishing YIN function of soothing the spirit, and is effective to prevent and treat anxiety neurosis, and is proved by zoopery, and relevant experimental data is as follows:
1 experimental drug: experimental group medicine of the present invention, the matched group diazepam tablets, specification: the 2.5mg/ sheet, the time spent pulverize is mixed with the 2.0mg/kg suspension with 0.5%CMC-Na solution; Thyroid tablet, specification: the 40mg/ sheet, time spent washing desaccharide clothing, pulverize is mixed with the 320mg/kg suspension with 0.5%CMC-Na solution.
2 laboratory animals
Kunming mouse, body weight is 20 ± 2g, and is male, and sub-cage rearing is 20 ± 2 ℃ animal housing, and the experimental mouse feed is the standard complete feed.
3 key instruments and reagent
3.1 key instrument
JY6002 type electronic balance: go up balance equipment factory of company of Nereid section
D2KW-C electronic thermostatic water-bath: the bright instrument plant in Beijing
Vacuum drying oven: North China, Tianjin experimental apparatus company limited
The temperature adjustable electrically heated cover of ZDHW type: emerging Instr Ltd. in the Huanghua City, Hebei province
Overhead cross labyrinth device and mice light and shade case apparatus: Chengdu TME Technology Co., Ltd.
3.2 main agents
Petroleum ether: Tianjin chemical reagent three factory's lot numbers: 20060226
N-butyl alcohol: Tianjin section close europeanized reagent development centre lot number: 20051020
4 confirmatory experiments
4.1 experimental design
Experiment is four factors with prepared rehmannia root polysaccharide, lily aqueous extract, wild jujube kernel alcohol extractive, Rhizoma Anemarrhenae ethanol extract, determine the optimal dose (middle dosage) of the nourishing YIN side of soothing the spirit with the synthesis result of orthonormal design of experiments and uniform Design experiment, its two times and/2nd are respectively high dose and low dosage.
4.2 be subjected to the configuration of reagent thing
Dosage according to design takes by weighing prepared rehmannia root polysaccharide, lily aqueous extract, wild jujube kernel alcohol extractive, Rhizoma Anemarrhenae ethanol extract, mix homogeneously, dissolved in distilled water configuration.
4.3 administering mode and animal grouping
90 of Male Kunming strain mice, be divided into 6 groups at random, every group 15, be respectively the high, medium and low dosage group of matched group, deficiency of YIN model group, diazepam group and prescription, every Mus is pressed 0.2ml/10g and irritates stomach, and wherein by the dosage administration of design, positive control drug is a 2.0mg/kg diazepam tablets suspension to the prescription group respectively, model contrast medicine is a 320mg/kg thyroid tablet suspension, all adopts the 0.2ml/10g gastric infusion.Every group the morning 8:00 except that matched group, all irritate stomach and give deficiency of YIN modeling medicine, matched group gives the suspension with volume 0.5%CMC-Na, the continuous irrigation stomach Ninth Heaven, in beginning in the 7th day, every afternoon, 15:00 was except that matched group and deficiency of YIN model group, give diazepam, prescription compatibility dosage respectively, matched group and deficiency of YIN model group give the suspension with volume 0.5%CMC-Na respectively, successive administration three days, 7:00 administration in the morning in the tenth day (with the 7th, eight, Ninth Heaven administration in afternoon), administration experimentized after 60 minutes.
4.4 mice elevated plus-maze test
60 minutes begin to test after the last administration.During experiment mice is placed labyrinth central authorities, head is towards opening arm, with the active situation of computer recording experiment periods (5 minutes) mice on overhead cross labyrinth.Entering number of times percentage ratio (OE%) with the mice open arms is that to enter number of times/(open arms enters number of times+closure arm and enters number of times) and open arms time of staying percentage ratio (OT%) be that open arms time of staying/(open arms entry time+closure arm entry time) is as estimating medicine index antianxity to open arms.
4.5 the mice light and shade is worn the case experiment
Begin experiment after 60 minutes in the last administration.Mice is placed camera-lucida central authorities, and the back of the body is towards the darkroom, and mice is worn the case number of times in the observed and recorded 10 minutes, with this as the index of estimating the medicine angst resistance effect.
In order to improve the accuracy of experimental result, begin a few days ago to stroke animal every day 1～2 minute in experiment usually, to reduce of the influence of irrelevant stress stimulation to this experiment.For avoiding in the experiment mice to hide and closing arm or camera bellows, usually mice is placed prologue to put into experimental provision in movable 5 minutes or 10 minutes later on again.
4.6 data statistics is handled
Each organize data information with mean ± standard deviation (expression of x ± s), the significance test between many groups is handled with one-way ANOVA, all data informations are all handled with the SPSS11.5 for windows of statistical software, P＜0.05, difference has significance.
5. result
Confirmatory experiment is to the influence of mice overhead cross labyrinth and the behavior of light and shade case (x ± s)
Normal group is compared △ P＜0.05, compares with model group,
*P＜0.05,
*P＜0.01,
* *P＜0.001
By table as can be seen, diazepam group and deficiency of YIN model group compare, and there is significance P＜0.05; Dosage group and deficiency of YIN model group compare in the prescription, and there is significance P＜0.05; And dosage group and diazepam group compare in the prescription, P＞0.05, and no significance illustrates the two there was no significant difference, can be with dosage replacing positive contrast medicine diazepam in the prescription, to overcome its untoward reaction.
6, conclusion
Clearly show that by above-mentioned situation the present invention has the significant nourishing YIN angst resistance effect of soothing the spirit, actual clinical meaning is arranged, it is succeeded in developing to people and prevents and treats anxiety, particularly the YIN-deficiency type anxiety provides the excellent drug condition, is that of Chinese medicine and pharmacy creates greatly, and its application benefits society with human.
Claims (5)
1. nourishing YIN granule of soothing the spirit is characterized in that this granule is to be made by Radix Rehmanniae Preparata 10-20g, Bulbus Lilii 10-15g, Semen Ziziphi Spinosae 15-25g, Rhizoma Anemarrhenae 5-10g.
2. the nourishing YIN according to claim 1 granule of soothing the spirit is characterized in that this granule is to be made by said Radix Rehmanniae Preparata 10g, Bulbus Lilii 12g, Semen Ziziphi Spinosae 20g, Rhizoma Anemarrhenae 6g.
3. the nourishing YIN according to claim 1 granule of soothing the spirit, it is characterized in that, be added with the adjuvant that starch, dextrin and stevioside are formed in said this granule, its adjuvant by starch, dextrin add again after with weight ratio preparation in 3: 1 starch and dextrin weight and 1/400 stevioside form.
4. the described nourishing YIN of the claim 1 particulate production method of soothing the spirit, it is characterized in that, at first with Radix Rehmanniae Preparata, Bulbus Lilii, the method that the Semen Ziziphi Spinosae and the Rhizoma Anemarrhenae adopt alcohol extraction or water to carry, produce prepared rehmannia root polysaccharide and ethanol extract or water extract, get prepared rehmannia root polysaccharide 0.3-1g then, lily aqueous extract 1-3g, wild jujube kernel alcohol extractive 0.2-0.5g, four kinds of raw material blendings of Rhizoma Anemarrhenae ethanol extract 0.2-0.4g, 1: 1 ratio adds adjuvant by weight, after four kinds of raw materials and the auxiliary materials and mixing, with mass concentration is that 70% ethanol is made soft material as solvent, sieve, extrusion granulator was put into 60 ℃ of oven dryings 3 hours.
5. a nourishing YIN particulate production method of soothing the spirit, it is characterized in that, after the auxiliary materials and mixing of forming by prepared rehmannia root polysaccharide 0.833g, lily aqueous extract 2.667g, wild jujube kernel alcohol extractive 0.4g, Rhizoma Anemarrhenae ethanol extract 0.333g with by the stevioside of starch 3.17475g, dextrin 1.058258g and 0.01058g, with mass concentration is that 70% ethanol is made soft material as solvent, sieve, extrusion granulator was put into 60 ℃ of oven dryings 3 hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200710054493A CN101062260B (en) | 2007-06-05 | 2007-06-05 | Buyin Yishen granule and production method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200710054493A CN101062260B (en) | 2007-06-05 | 2007-06-05 | Buyin Yishen granule and production method thereof |
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