CN101023729A - Raspberry quick-breeding method - Google Patents
Raspberry quick-breeding method Download PDFInfo
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- CN101023729A CN101023729A CN 200610041844 CN200610041844A CN101023729A CN 101023729 A CN101023729 A CN 101023729A CN 200610041844 CN200610041844 CN 200610041844 CN 200610041844 A CN200610041844 A CN 200610041844A CN 101023729 A CN101023729 A CN 101023729A
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- bud
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- 235000011034 Rubus glaucus Nutrition 0.000 title claims abstract description 21
- 235000009122 Rubus idaeus Nutrition 0.000 title claims abstract description 21
- 238000009395 breeding Methods 0.000 title claims abstract description 19
- 244000235659 Rubus idaeus Species 0.000 title description 4
- 240000007651 Rubus glaucus Species 0.000 claims abstract description 17
- 230000001488 breeding effect Effects 0.000 claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 9
- 239000002689 soil Substances 0.000 claims abstract description 8
- 239000004576 sand Substances 0.000 claims abstract description 7
- 238000005286 illumination Methods 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims description 35
- 238000002360 preparation method Methods 0.000 claims description 23
- 230000001902 propagating effect Effects 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 11
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 9
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 235000016709 nutrition Nutrition 0.000 claims description 7
- 230000035764 nutrition Effects 0.000 claims description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- 240000007594 Oryza sativa Species 0.000 claims description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 235000009566 rice Nutrition 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 229930003427 Vitamin E Natural products 0.000 claims description 4
- 235000013339 cereals Nutrition 0.000 claims description 4
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 4
- 235000019165 vitamin E Nutrition 0.000 claims description 4
- 239000011709 vitamin E Substances 0.000 claims description 4
- 229940046009 vitamin E Drugs 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 229930003268 Vitamin C Natural products 0.000 claims description 3
- 239000003963 antioxidant agent Substances 0.000 claims description 3
- 230000003078 antioxidant effect Effects 0.000 claims description 3
- 235000006708 antioxidants Nutrition 0.000 claims description 3
- 239000012752 auxiliary agent Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 235000019160 vitamin B3 Nutrition 0.000 claims description 3
- 239000011708 vitamin B3 Substances 0.000 claims description 3
- 235000019154 vitamin C Nutrition 0.000 claims description 3
- 239000011718 vitamin C Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 2
- 230000004313 glare Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 210000003739 neck Anatomy 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 3
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- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
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- 244000082204 Phyllostachys viridis Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
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- 239000011425 bamboo Substances 0.000 description 2
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- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
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- 230000015572 biosynthetic process Effects 0.000 description 1
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- 201000011510 cancer Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
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- 230000001172 regenerating effect Effects 0.000 description 1
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- 239000010959 steel Substances 0.000 description 1
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Landscapes
- Cultivation Of Plants (AREA)
Abstract
The present invention discloses a rapid breeding method of raspberry. Said method includes the following steps: selecting and using normally-developed raspberry shoot with leaf, said shoot length is 6 mm; soaking said raspberry shoot in breeding solution for 1-2 hr; utilizing neck-treating agent to treat two end necks of said raspberry shoot, making neck-closing treatment; inoculating said shoot on the seedling-cultivating bed, lower layer of said seedling-cultivating bed is nutrient soil whose thickness is 3 cm, and the upper layer of said seedling-cultivating bed is fine sand layer whose thickness is 3 m; putting up an arch shed over the seedling-cultivating bed, the temperature of shed interior is 15-33 deg.C, humidity is 90% and illumination rate is 30%; after 20 days a whole plant can be formed.
Description
Technical field
The present invention relates to a kind of plant propagation renovation process, especially raspberry microbody material method for quickly breeding.
Background technology
Raspberry is perennial shrub type fruit tree, the tender succulence of fruit, and unique flavor, color and luster is tempting, and especially its distinctive fragrance and unique health-care effect cause that common people pay close attention to.Raspberry really is rich in each seed amino acid, wherein has eight kinds to be the amino acid of needed by human.Vitamin C, vitamin E in the raspberry fruit, superoxide dismutase acid (SOD) y-aminobutyric acid, tanning acid etc. are anti-ageing, cancer-resisting substance content is all very abundant.
The breeding of raspberry has traditional root turion plant division, and cuttage and method for tissue culture are though traditional cuttage or plant division method are not high to its specification requirement, grasp easily, still, its reproduction speed is slow, to maternal material requirements height, consumption is big, survival rate is low, also is subjected to the restriction in season; If do the breeding of raspberry according to existing method for tissue culture, must in the laboratory, carry out so, need corresponding apparatus, the seedling quantity of cultivation is also limited, and not only cost is too high, and can't satisfy the wilderness demand of actual production.
Summary of the invention
The objective of the invention is to propose a kind of can be in the method for big field industrial fast speed propagating raspberry, its is not only grasped easily and uses, and the plant of breeding unified complete, survival rate is high, also should not be subject to seasonal restrictions.
The technical scheme that realizes above-mentioned purpose is to carry out as follows:
(1) the grow seedlings bed and the preparation of seedling growth greenhouse:
Grow seedlings the bed long 15-20 rice, wide 4-5 rice, excavate wide 0.3 meter at wide 1/2nd places of bed surface, one in dark 0.3 meter ditch, on the seedbed, spread the nutrition soil of 3 centimetres of bed thickness earlier, again at the fine sand grain of nutrition soil upper berth 3 cm thicks as the breeding bed surface, above this seedbed, take shed, the canopy face adopts thick 0.8mm agricultural film; After the bed surface of growing seedlings in the shed performs, to the environment in the canopy and the bed sterilization that carries out disinfection of growing seedlings, and a sand bed two shut, it is standby to shine the two ends of raising canopy after 48 hours with solar glare.
(2) preparation of propagating liquid:
Shine the grand 3000mg of benzene
Auxiliary agent DA-6 1800mg
Vitamin C 260mg
Antioxidant vitamin E 240mg
Preparation main points: take by weighing each material according to above-mentioned weight of material, all join in the medicinal alcohol of 100ml 95% and dissolve, add the warm boiling water of 1000ml during use again.
(3) preparation of raspberry otch material processed agent:
Cobastab
2, Cobastab
6, vitamin PP, starch, sterilization ling
Proportioning is respectively: 3: 4: 4: 88: 1
Preparation main points: take by weighing each material according to above-mentioned material proportion, stir, leave the shady and cool ventilation place in.
(4) female parent is bred the preparation of material:
Earlier intercepting 4-6cm is long, leaf bud is full, normotrophic band leaf raspberry branch, again 2mm place, the leaf bud of this material top is cut into 45, then this bud joint is immersed in the propagating liquid of above-mentioned (2) preparing 1-2 hour:
(5) inoculation:
Take out female parent and breed material from propagating liquid, dried in the air 5-10 minute, the two ends otch to band leaf bud joint seals processing with the otch material processed agent of above-mentioned (3) preparation again, then it is seeded on the husky bed according to spacing 5 * 5cm;
(6) postvaccinal management:
After bud joint is seeded in husky bed and goes up in 20 days, should control that temperature of shed is 90% at 15-33 ℃, humidity, the illumination rate is 30%.
According to the method described above, the material cut place began to take root in the 15th day, the whole plant that just had formation to regenerate in 25 days.Beneficial effect of the present invention is:
(1) technology is simple, and reproduction speed is fast, the installations and facilities less investment, and cost is low, is easy to promote;
(2) save maternal propagating materials, no matter south, the north can be bred throughout the year;
(3) carry out the quick propagation production of batch production in big field, reproduction coefficient becomes how much multiples to increase easily large-scale production:
(4) seedling of breeding fast, well developed root system, seedling are strengthened, survival rate is high, do not have variation.
Embodiment
Below in conjunction with embodiment the present invention is done description in further detail:
Embodiment 1
(1) the grow seedlings bed and the preparation of seedling growth greenhouse:
One of arrangement is long 15 meters on the level land, the wide 4 meters bed of growing seedlings, vertically one of excavation is wide 0.3 meter at wide 1/2nd places of bed surface, dark 0.3 meter ditch, the brick one-tenth of this ditch, on this seedbed, spread the nutrition soil of a bed thickness 3cm earlier, again at the thick fine sand grain of nutrition soil upper berth 3cm as the breeding bed surface, after the bed of growing seedlings is carried out, take a shed with bamboo chip or steel pipe above it, the highest top of canopy is a 1.5-1.7 rice, the canopy face adopts thick 0.8mm film, land around the canopy face,, at last the film at breeding bed two is hung down the breeding bed is shut then with the bed surface disinfection.When fine, by solar light irradiation 48 hours, control breeding bed tempertaure reached 50 ℃, and the two ends of raising canopy then are standby.
(2) preparation of propagating liquid:
Shine the grand 3000mg of benzene
Auxiliary agent DA-6 1800mg
Vitamin 260mg
Antioxidant vitamin E 240mg
Above-mentioned material mixes according to quantity, and elder generation dissolves with 95% alcohol, adds water to 1000ml then.
(3) preparation of raspberry otch material processed agent:
Cobastab
2, Cobastab
6, vitamin PP, starch, sterilization ling
Proportioning is respectively: 3: 4: 4: 88: 1
Above-mentioned material mixes in proportion, and leaves the shady and cool ventilation place in.
(4) female parent is bred the preparation of material:
Vertical intercepting 6cm is long, leaf bud is full, normotrophic band leaf raspberry branch, branch after exsomatizing will in time keep the skin wet, again 2mm place in the leaf bud of this material top is cut into 45 ℃ of angles, below 3mm vertically cuts off at place, this bud is saved to be immersed in the propagating liquid of above-mentioned (2) preparing 1 to 2 hour then.
(5) inoculation:
Take out female parent and breed material from propagating liquid, dried in the air 10 minutes, the two ends otch to band leaf bud joint seals it with the otch material processed agent of above-mentioned (3) preparation again, then the bud joint is seeded on the breeding bed according to spacing 5 * 5cm.During inoculation, the lower end notch portion of bud joint is imbedded in the fine sand grain of breeding bed, and the leaf bud of parent and upper cut position are exposed.
(6) postvaccinal management:
Should to control temperature of shed after the breeding bed is gone up be 90% at 15-33 ℃, humidity when bud joint is seeded in, and the illumination rate is 30%.The material cut place just began to take root in the 15th day, just can form the whole plant of regenerating in 25 days.
Embodiment 2
(1) the grow seedlings bed and the preparation of seedling growth greenhouse:
Long 15 meters, wide 4 meters bed of growing seedlings of arrangement on the level land vertically excavates wide 0.3 meter, dark 0.3 a meter ditch at wide 1/2nd places of bed surface, takes a shed with bamboo chip above it, and the highest top of canopy is a 1.5-1.7 rice, and the canopy face adopts thick 0.8mm film.
(2) preparation of propagating liquid:
Finish according to embodiment 1 described step;
(3) preparation of raspberry otch material processed agent:
Finish according to embodiment 1 described step;
(4) female parent is bred the preparation of material:
Finish according to embodiment 1 described step;
(5) inoculation:
Take out female parent and breed material from propagating liquid, dried in the air 10 minutes, the two ends otch to band leaf bud joint seals it with the agent of otch material processed again, then the bud joint is seeded in the nutrient cup that nutrition soil is housed.
(6) inoculation back management:
After bud joint is seeded in the nutrient cup, nutrient cup is placed on the bed of growing seedlings according to spacing 5 * 5cm or 4 * 4cm, the control temperature of shed is 90% at 15-33 ℃, humidity, the illumination rate is 30%.
Claims (1)
1. raspberry quick-breeding method is characterized in that carrying out as follows:
(1) the grow seedlings bed and the preparation of seedling growth greenhouse:
Grow seedlings the bed long 15-20 rice, wide 4-5 rice, excavate wide 0.3 meter at wide 1/2nd places of bed surface, one in dark 0.3 meter ditch, on the seedbed, spread the nutrition soil of 3 centimetres of bed thickness earlier, again at the fine sand grain of nutrition soil upper berth 3 cm thicks as the breeding bed surface, above this seedbed, take shed, the canopy face adopts thick 0.8mm agricultural film; After the bed surface of growing seedlings in the shed performs, to the environment in the canopy and the bed sterilization that carries out disinfection of growing seedlings, and a sand bed two shut, it is standby to shine the two ends of raising canopy after 48 hours with solar glare;
(2) preparation of propagating liquid:
Shine the grand 3000mg of benzene
Auxiliary agent DA-6 1800mg
Vitamin C 260mg
Antioxidant vitamin E 240mg
Preparation main points: take by weighing each material according to above-mentioned weight of material, all join in the medicinal alcohol of 100ml95% and dissolve, add the warm boiling water of 1000ml during use again;
(3) preparation of raspberry otch material processed agent:
Material: Cobastab
2, Cobastab
6, vitamin PP, starch, sterilization ling
Proportioning is: 3: 4: 4: 88: 1
Preparation main points: take by weighing each material according to above-mentioned material proportion, stir, leave the shady and cool ventilation place in;
(4) female parent is bred the preparation of material:
Earlier intercepting 4-6cm is long, leaf bud is full, normotrophic band leaf raspberry branch, again the branch at 2mm place, the leaf bud of this material top is cut into 45, then this bud joint is immersed in the propagating liquid of above-mentioned (2) preparing 1-2 hour;
(5) inoculation:
Take out female parent and breed material from propagating liquid, dried in the air 5-10 minute, the two ends otch to the bud joint seals processing with the otch material processed agent of above-mentioned (3) preparation again, then it is seeded on the husky bed according to spacing 5 * 5cm;
(6) postvaccinal management:
After bud joint is seeded in husky bed and goes up in 20 days, should control that temperature of shed is 90% at 15-33 ℃, humidity, the illumination rate is 30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610041844 CN101023729A (en) | 2006-02-21 | 2006-02-21 | Raspberry quick-breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610041844 CN101023729A (en) | 2006-02-21 | 2006-02-21 | Raspberry quick-breeding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101023729A true CN101023729A (en) | 2007-08-29 |
Family
ID=38742632
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610041844 Pending CN101023729A (en) | 2006-02-21 | 2006-02-21 | Raspberry quick-breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101023729A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477985A (en) * | 2013-09-09 | 2014-01-01 | 华南农业大学 | Regeneration culture medium and culture method for improving regenerated adventitious buds of echinacea explants |
| CN104145663A (en) * | 2014-07-25 | 2014-11-19 | 宁国市瑞龙生态农林开发有限公司 | Ecological imitated rubus idaeus planting method |
| CN106171374A (en) * | 2016-06-29 | 2016-12-07 | 崔素清 | A kind of cuttage breeding method of rasp berry |
| WO2017166567A1 (en) * | 2016-04-02 | 2017-10-05 | 江苏辉丰农化股份有限公司 | Synergistic plant growth regulator composition |
| CN108605786A (en) * | 2018-04-10 | 2018-10-02 | 宁夏农林科学院枸杞工程技术研究所 | A method for making wolfberry bonsai with hairy roots of old pile branches |
-
2006
- 2006-02-21 CN CN 200610041844 patent/CN101023729A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477985A (en) * | 2013-09-09 | 2014-01-01 | 华南农业大学 | Regeneration culture medium and culture method for improving regenerated adventitious buds of echinacea explants |
| CN103477985B (en) * | 2013-09-09 | 2015-08-19 | 华南农业大学 | A kind of regeneration culture medium and cultural method improving echinacea purpurea explant regeneration indefinite bud |
| CN104145663A (en) * | 2014-07-25 | 2014-11-19 | 宁国市瑞龙生态农林开发有限公司 | Ecological imitated rubus idaeus planting method |
| WO2017166567A1 (en) * | 2016-04-02 | 2017-10-05 | 江苏辉丰农化股份有限公司 | Synergistic plant growth regulator composition |
| CN106171374A (en) * | 2016-06-29 | 2016-12-07 | 崔素清 | A kind of cuttage breeding method of rasp berry |
| CN108605786A (en) * | 2018-04-10 | 2018-10-02 | 宁夏农林科学院枸杞工程技术研究所 | A method for making wolfberry bonsai with hairy roots of old pile branches |
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Open date: 20070829 |