CN100381810C - Fluorometric auxiliary detection device - Google Patents
Fluorometric auxiliary detection device Download PDFInfo
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- CN100381810C CN100381810C CNB2003101218265A CN200310121826A CN100381810C CN 100381810 C CN100381810 C CN 100381810C CN B2003101218265 A CNB2003101218265 A CN B2003101218265A CN 200310121826 A CN200310121826 A CN 200310121826A CN 100381810 C CN100381810 C CN 100381810C
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Abstract
The present invention relates to a fluorescence auxiliary detection device. The excitation light emitted by a light source module is used for radiating an object to be detected respectively by a collimation lens, a light splitting mirror and a first focusing lens, and thereby, the fluorescence to be detected is correspondingly emitted, and then the fluorescence is received by a filter lens group, a second focusing lens and a light detector to generate light current signals which are transferred to a data processing device so as to make detection and analysis operation. The fluorescence auxiliary detection device has the advantages of small volume, simple structure, low production cost, etc.
Description
Technical field
The present invention relates to a kind of auxiliary detection device, be applied to biomedical association area, relate in particular to a kind of fluoroscopic assist pick-up unit.
Background technology
Science and technology such as biomedicine has great breakthrough along with the step of world's progress in recent years, and cause is the rise of semiconductor industry, and the research and development that causes related electronic components to continue is rapid, and drives the also last layer more of research on the biomedicine.
The detection technique of biomedical sector wherein, it is present research emphasis, conventional detection, be that biochip is placed on the discs with data layer, and shine with the light of specific wavelength, the fluorescence signal that then utilizes the discs reading device to read biochip simultaneously to be distributed is with the data layer signal of discs data layer, at last via data processing unit with fluorescence signal and data layer signal, and rebuild the fluorescence signal of the biochip of two-dimensional format.United States Patent (USP) is announced No. 6320660 in addition, and also disclosing has relevant technology.
Except above-mentioned detection mode, (electrophoresis EP) carries out the technology of all kinds of detections, is also used widely to utilize electrophoresis.Wherein the ultimate principle of electrophoresis is the dissociation at any material itself, or surperficial other charging point of absorption of going up, and just can move to certain electrode in electric field.As charged particle, can be little ion, also can be biomacromolecule, protein, nucleic acid, virion.For example the amino acid sub-cell body of constitutive protein matter is an amphiprotic substance, and it is charged to dissociate under certain pH condition, and forms charge source.And this charged particle will move electrophoresis that Here it is towards opposite electrical electrode under electric field action.
In coherent detection fields such as biomedicine, principle of electrophoresis is also adopted widely, wherein imposes high voltage at kapillary, and be filled in the determinand in the kapillary this moment, just can produce electrophoresis; Follow nuclifort (the deoxyribonucleic acid of determinand, DNA) will combine with the fluorescent material that adds, see through the irradiation of light emitting sources such as laser, will produce the fluorescence of different wave length, just arrive the related gene characteristic and the concentration data of determinand whereby as can be known, and produce the usefulness that development for deliberation is reported in check and analysis.
Yet the required common complex structural designs of fluorescence detection device of this Capillary Electrophoresis genetic analysis process is bulky, and product price is quite expensive with relevant maintenance cost, moreover the procurement funding of research institution is limited usually; Therefore this is very big puzzlements for numerous researchers, also is the problem that related industry is endeavoured to improve.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of fluoroscopic assist pick-up unit, have simple in structure, volume is little, and modular design can detect a plurality of determinands simultaneously, and has advantages such as price is cheap.
To achieve these goals, the invention provides a kind of fluoroscopic assist pick-up unit, in order to shine a determinand and receive this determinand corresponding distribute one detect fluorescence, its characteristics are, include:
One light source module is in order to launch an exciting light;
One collimating mirror is assemblied in a side of this light source module, in order to receive and to change this exciting light, so that this exciting light advances with parallel mode;
One spectroscope is arranged at a side of this collimating mirror, so that parallel this exciting light that advances turns to refraction;
One first condenser lens is assemblied in this spectroscopical side, focuses on this determinand of irradiation in order to this exciting light after will reflecting, and makes this determinand correspondence distribute this detection fluorescence;
Wherein this detection fluorescence is to see through this first condenser lens conversion and to advance near parallel mode, penetrate this spectroscope again;
One optical filtering group is assemblied in this spectroscopical opposite side, filtering parasitic light and bias light, and passes through only for this detection fluorescence of a preset range wavelength;
One second condenser lens is assemblied in a side of this optical filtering group, focuses in order to this detection fluorescence with the preset range wavelength; And
One optical detector is equipped on a side of this second condenser lens, detects fluorescence in order to behind the collectiong focusing this, and to change this detection fluorescence be a photocurrent signal.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics are, also include a photoelectricity signal modular converter, include:
One smooth signal conversion unit is in order to receive this photocurrent signal and to be converted to a voltage signal;
One amplifier is in order to receive and to amplify this voltage signal; And
One digital signal converting unit is in order to be converted to a digital signal with this voltage signal.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics are that this light source module is a laser diode.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics be, this spectroscope receives reflecting the plane of this exciting light, is 45 degree angles with the incident direction of this exciting light.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics be, this optical filtering group of received should detect the plane of fluorescence, is 90 with the incident direction of this detections fluorescence and spends angles.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics are that this optical filtering group is an optical band pass optical filtering.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics are that this spectroscope is a pair of measuring spectroscope.
Another kind of fluoroscopic assist pick-up unit of the present invention includes a light source module, a collimating mirror, a spectroscope, one first condenser lens, an optical filtering group, one second condenser lens and an optical detector; Wherein this light source module is launched an exciting light to this collimating mirror and is received and change this exciting light and advance with parallel mode, continuous this exciting light being turned to by this spectroscope reflexes to this first condenser lens, and this determinand of focusing irradiation, cause this determinand correspondence to distribute one and detect fluorescence, see through this first condenser lens again and make this detection fluorescence be near parallel advancing, and penetrate this spectroscope filtered and limited a preset range wavelength by this optical filtering group detection fluorescence and pass through, utilize this second condenser lens should detect fluorescence and focus on, receive by this optical detector again and be converted to a photocurrent signal.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics are, also include a photoelectricity signal modular converter, include:
One smooth signal conversion unit is in order to receive this photocurrent signal and to be converted to a voltage signal;
One amplifier is in order to receive and to amplify this voltage signal; And
One digital signal converting unit is in order to be converted to a digital signal with this voltage signal.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics are that this light source module is a laser diode.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics be, this spectroscope receives the plane of this exciting light of reflection, is 45 degree angles with the incident direction of this exciting light.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics be, this optical filtering group of received should detect the plane of fluorescence, is 90 with the incident direction of this detections fluorescence and spends angles.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics are that this optical filtering group is an optical band pass optical filtering.
Above-mentioned fluoroscopic assist pick-up unit, its characteristics are that this spectroscope is a pair of measuring spectroscope.
According to the disclosed a kind of fluoroscopic assist pick-up unit of the present invention, consist predominantly of light source module, collimating mirror, spectroscope, first condenser lens, optical filtering group, second condenser lens and optical detector.Wherein light source module is in order to launching excitation light, and collimating mirror is assemblied in a side of light source module, in order to reception and conversion exciting light, and exciting light is advanced with parallel mode.Spectroscope is arranged at a side of collimating mirror, and with the working direction shape of exciting light in angle of 45 degrees so that exciting light turns to reflection.First condenser lens is installed in a spectroscopical side, focuses on and the irradiation determinand with the exciting light after reflection is turned to, and this moment, determinand thereby correspondence were distributed detection fluorescence.
Then, detect fluorescence and will collect and approaching parallel advancing, and penetrate spectroscope through first condenser lens.Optical filtering assembling is assigned in a spectroscopical side, can receive and penetrate spectroscopical detection fluorescence, and make the detection wavelength of fluorescence that penetrates within limits so as to stopping parasitic light.Second detect lens focus in order to the detection fluorescence after will filtering concentrated, at last by being equipped on the optical detector of second condenser lens, one side, the detection fluorescence behind the receiving filtration and be converted to the photocurrent signal.And this photocurrent signal can pass through photoelectricity signal modular converter, is converted to digital signal and is passed to data processing equipments such as computing machine, can carry out analyzing and testing.
Fluoroscopic assist pick-up unit of the present invention, can be applicable to all kinds of fluorescence detection devices such as Capillary Electrophoresis genetic analysis instrument, genetic chip and protein-chip, it is simple to have small volume structure, modular design and the advantage that can detect a plurality of determinands simultaneously, and correlated parts is obtained easily and can effectively be reduced production costs.
Describe the present invention below in conjunction with the drawings and specific embodiments, but not as a limitation of the invention.
Description of drawings
Fig. 1 is the synoptic diagram of fluoroscopic assist pick-up unit of the present invention;
Fig. 2 is the synoptic diagram of fluoroscopic assist pick-up unit of the present invention and photoelectricity signal modular converter; And
Fig. 3 is the use synoptic diagram of the modularization fluorescence auxiliary detection device of fluoroscopic assist pick-up unit of the present invention.
Embodiment
According to the disclosed a kind of fluoroscopic assist pick-up unit of the present invention, please refer to Fig. 1 and Fig. 2, consist predominantly of light source module 10, collimating mirror 20, spectroscope 30, first condenser lens 40, determinand 50, optical filtering group 60, second condenser lens 70 and optical detector 80.Wherein light source module 10 enforceable kinds are a lot, and the mercury lamp of gas laser and continuous spectrum etc. is mostly adopted in the commercially available prod.Yet it costs an arm and a leg this type of light source module, and the serviceable life of mercury lamp is short, so the present invention recommends to use price cheap in comparison many, and the close laser diode (Laser Diode) of function is used as light source module 10.Light source module 10 is in order to launching excitation light, and collimating mirror (Collimator) 20 is assemblied in a side of light source module 10, in order to receive and the conversion exciting light, so that exciting light advances with parallel mode.Spectroscope 30 is inclined in a spectroscopical side, so that the parallel exciting light that advances turns to refraction, owing to spectroscope 30 has the different characteristics and the gradient of its installation according to its product specification, the present invention adopts dual wave length spectrophotometry mirror (Dichroic Mirror), and it receives the plane of exciting light and the incident direction of exciting light is 45 degree angles.First condenser lens 40 is a kind of aspheric surface object lens, puts in a spectroscopical side, and is positioned on the light path of the exciting light after the refraction, in order to the exciting light after this refraction is focused on irradiation determinand 50.Determinand 50 in this preferred embodiment imposes external high voltage, and makes inner filling material produce electrophoresis, therefore correspondence is distributed detection fluorescence behind excitation light irradiation.In other words, can produce Stoke ' s Shift effect behind this excitation light irradiation determinand 50, radiation (Emission) goes out the detection fluorescence longer than excitation wavelength.Detect then that fluorescence will be once again sees through that first condenser lens 40 is collected and advancing, and pass spectroscope 30 in the mode that almost completely penetrates near parallel mode.
Continue as shown in Figure 2,, therefore must see through extra photoelectricity signal modular converter and finish because the photocurrent signal that optical detector 80 provided can not directly be read by data processing equipment 120.Photoelectricity signal modular converter is made up of light signal conversion unit 90, amplifier 100, digital signal converting unit 110.Light signal conversion unit 90 is in order to receive the photocurrent signal that optical detector 80 is transmitted, and be converted to voltage signal, then see through amplifier 100 and receive and amplify voltage signal, see through digital signal converting unit 110 at last, voltage signal is converted to digital signal; And this digital signal can be accepted by data processing equipments such as computing machine, electrophoresis genetic analysis instrument, and can carry out check and analysis to the detection fluorescence that determinand 50 is distributed.
Fluoroscopic assist pick-up unit provided by the present invention also can carry out modular design, as shown in Figure 3, said modules can be packaged in the mechanism to form modularization fluorescence auxiliary detection device 200, and the fluoroscopic examination analysis that can be correlated with to a plurality of determinands 50 simultaneously, and the related data that the detection fluorescence of each determinand 50 is produced is passed to data processing equipment 120 carries out check and analysis.
Certainly; the present invention also can have other various embodiments; under the situation that does not deviate from spirit of the present invention and essence thereof; those of ordinary skill in the art work as can make various corresponding changes and distortion according to the present invention, but these corresponding changes and distortion all should belong to the protection domain of the appended claim of the present invention.
Claims (14)
1. fluoroscopic assist pick-up unit, in order to shine a determinand and receive this determinand corresponding distribute one detect fluorescence, it is characterized in that, include:
One light source module is in order to launch an exciting light;
One collimating mirror is assemblied in a side of this light source module, in order to receive and to change this exciting light, so that this exciting light advances with parallel mode;
One spectroscope is arranged at a side of this collimating mirror, so that parallel this exciting light that advances turns to refraction;
One first condenser lens is assemblied in this spectroscopical side, focuses on this determinand of irradiation in order to this exciting light after will reflecting, and makes this determinand correspondence distribute this detection fluorescence;
Wherein this detection fluorescence is to see through this first condenser lens conversion and to advance near parallel mode, penetrate this spectroscope again;
One optical filtering group is assemblied in this spectroscopical opposite side, filtering parasitic light and bias light, and passes through only for this detection fluorescence of a preset range wavelength;
One second condenser lens is assemblied in a side of this optical filtering group, focuses in order to this detection fluorescence with the preset range wavelength; And
One optical detector is equipped on a side of this second condenser lens, detects fluorescence in order to behind the collectiong focusing this, and to change this detection fluorescence be a photocurrent signal.
2. fluoroscopic assist pick-up unit according to claim 1 is characterized in that, also includes a photoelectricity signal modular converter, includes:
One smooth signal conversion unit is in order to receive this photocurrent signal and to be converted to a voltage signal;
One amplifier is in order to receive and to amplify this voltage signal; And
One digital signal converting unit is in order to be converted to a digital signal with this voltage signal.
3. fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this light source module is a laser diode.
4. fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this spectroscope receives reflecting the plane of this exciting light, is 45 degree angles with the incident direction of this exciting light.
5. fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this optical filtering group of received should detect the plane of fluorescence, is 90 with the incident direction of this detections fluorescence and spends angles.
6. fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this optical filtering group is an optical band pass optical filtering.
7. fluoroscopic assist pick-up unit according to claim 1 is characterized in that, this spectroscope is a pair of measuring spectroscope.
8. a fluoroscopic assist pick-up unit includes a light source module, a collimating mirror, a spectroscope, one first condenser lens, an optical filtering group, one second condenser lens and an optical detector; Wherein this light source module is launched an exciting light to this collimating mirror and is received and change this exciting light and advance with parallel mode, continuous this exciting light being turned to by this spectroscope reflexes to this first condenser lens, and this determinand of focusing irradiation, cause this determinand correspondence to distribute one and detect fluorescence, see through this first condenser lens again and make this detection fluorescence be near parallel advancing, and penetrate this spectroscope filtered and limited a preset range wavelength by this optical filtering group detection fluorescence and pass through, utilize this second condenser lens should detect fluorescence and focus on, receive by this optical detector again and be converted to a photocurrent signal.
9. fluoroscopic assist pick-up unit according to claim 8 is characterized in that, also includes a photoelectricity signal modular converter, includes:
One smooth signal conversion unit is in order to receive this photocurrent signal and to be converted to a voltage signal;
One amplifier is in order to receive and to amplify this voltage signal; And
One digital signal converting unit is in order to be converted to a digital signal with this voltage signal.
10. fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this light source module is a laser diode.
11. fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this spectroscope receives the plane of this exciting light of reflection, is 45 degree angles with the incident direction of this exciting light.
12. fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this optical filtering group of received should detect the plane of fluorescence, was 90 with the incident direction of this detections fluorescence and spent angles.
13. fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this optical filtering group is an optical band pass optical filtering.
14. fluoroscopic assist pick-up unit according to claim 8 is characterized in that, this spectroscope is a pair of measuring spectroscope.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101218265A CN100381810C (en) | 2003-12-19 | 2003-12-19 | Fluorometric auxiliary detection device |
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| CNB2003101218265A CN100381810C (en) | 2003-12-19 | 2003-12-19 | Fluorometric auxiliary detection device |
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| CN1629620A CN1629620A (en) | 2005-06-22 |
| CN100381810C true CN100381810C (en) | 2008-04-16 |
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| CNB2003101218265A Expired - Fee Related CN100381810C (en) | 2003-12-19 | 2003-12-19 | Fluorometric auxiliary detection device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8119066B2 (en) * | 2006-02-08 | 2012-02-21 | Molecular Devices, Llc | Multimode reader |
| CN101535796B (en) * | 2006-11-03 | 2013-05-01 | 图德·阿尔温特 | Method and apparatus for evaluating a sample |
| CN102375615A (en) * | 2010-08-20 | 2012-03-14 | 硕擎科技股份有限公司 | Laser optical contact module |
| US9471179B2 (en) | 2010-08-20 | 2016-10-18 | Serafim Technologies Inc. | Laser optical touch control module analog-to-digital conversion system and method of the same |
| CN102645425A (en) * | 2011-02-21 | 2012-08-22 | 中国检验检疫科学研究院 | A ray machine structure of a portable laser spectral analyzer |
| CN102488564B (en) * | 2011-12-15 | 2013-12-18 | 杭州电子科技大学 | Dental plaque detector capable of imaging by aid of fluorescent area arrays |
| US9978341B2 (en) | 2014-12-23 | 2018-05-22 | Mediatek Inc. | Visual data processing method and visual data processing system which can perform a process operation according to a gazing point |
| CN109799219A (en) * | 2019-03-13 | 2019-05-24 | 浙江荷清柔性电子技术有限公司 | Oxygen detection device and system |
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|---|---|---|---|---|
| US4855243A (en) * | 1983-09-26 | 1989-08-08 | H.S.G. Venture (A Joint Venture Partnership) | Neurological and biological molecular electro-optical devices and methods |
| CN1184247A (en) * | 1996-12-06 | 1998-06-10 | 三星电子株式会社 | Apparatus for measuring degree of inclination of objective lens for optical pickup |
| CN1311436A (en) * | 2000-03-01 | 2001-09-05 | 上海和泰光电科技有限公司 | Reading of biological chip fluorescent image on rotary platform |
| CN1317692A (en) * | 2000-04-10 | 2001-10-17 | 财团法人工业技术研究院 | Biochip tester |
| TW495748B (en) * | 2000-10-17 | 2002-07-21 | Ind Tech Res Inst | Signal detecting method and device of biochip |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855243A (en) * | 1983-09-26 | 1989-08-08 | H.S.G. Venture (A Joint Venture Partnership) | Neurological and biological molecular electro-optical devices and methods |
| CN1184247A (en) * | 1996-12-06 | 1998-06-10 | 三星电子株式会社 | Apparatus for measuring degree of inclination of objective lens for optical pickup |
| CN1311436A (en) * | 2000-03-01 | 2001-09-05 | 上海和泰光电科技有限公司 | Reading of biological chip fluorescent image on rotary platform |
| CN1317692A (en) * | 2000-04-10 | 2001-10-17 | 财团法人工业技术研究院 | Biochip tester |
| TW495748B (en) * | 2000-10-17 | 2002-07-21 | Ind Tech Res Inst | Signal detecting method and device of biochip |
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