CN109956801A - A kind of liquid fertilizer and preparation method thereof for fruit corn flower bud development - Google Patents
A kind of liquid fertilizer and preparation method thereof for fruit corn flower bud development Download PDFInfo
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- CN109956801A CN109956801A CN201711426418.9A CN201711426418A CN109956801A CN 109956801 A CN109956801 A CN 109956801A CN 201711426418 A CN201711426418 A CN 201711426418A CN 109956801 A CN109956801 A CN 109956801A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The liquid fertilizer and preparation method thereof that the invention discloses a kind of for fruit corn flower bud development, the liquid fertilizer include 1000 parts of active liquid fertilizer substrate preparation, multiple 1 ~ 10 part of phthalein nucleic acid, 0.01 ~ 3 part of chitosan oligosaccharide, 52 ~ 65 parts of urea, 1 ~ 8 part of ammonium hydrogencarbonate, 8 ~ 32 parts of amino acid 1,64 ~ 78 parts of potassium sulfate, 2 ~ 12 parts of boric acid, calcium superphosphate 35 ~ 48,0.1 ~ 5 part of calcium gluconate, 0.1 ~ 5 part of white vitriol；The liquid fertilizer has not only effectively facilitated the flower bud development of fruit corn, while having selectivity to the Inflorescence development of different corns, the most significant to the bud differentiation effect of fruit corn.
Description
Technical field
The invention belongs to the technical field of liquid fertilizer, more particularly, to a kind of for fruit corn flower bud development
Liquid fertilizer and preparation method thereof.
Background technique
Fruit corn is a kind of super-sweet corn for being suitble to eat it raw, and green stick stage thin skin, juice is more, matter is crisp and sweet tea, can directly be given birth to
Eat, for very thin epidermis once stinging brokenly, the juice of faint scent overflows tooth cheek, eat it raw it is ripe eat all special sweet tea, especially crisp, as fruit,
Therefore it is referred to as " fruit corn ".Fruit corn belongs to one of corn, is the developed countries such as American-European, South Korea and Japan
One of main vegetables, because that it is with nutrition abundant, sweet tea, fresh, crisp, tender characteristic is deep by each stratum consumers.Production
In corn can be divided into conventional corn, super-sweet corn and reinforced corn three classes, super-sweet corn is high, suitable due to sugar content
Suitable picking time is long and is planted extensively.Corn also has cultivation in China.Plant is shorter, and tillering ability is stronger.On fruit ear bract
There is boot leaf.Seed is yellowish or white, and embryo is larger, and milk stage is tender in fact, is rich in water-soluble polysaccharide, vitamin A, vitamin C, rouge
Fat and protein etc..
Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out
Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto-
Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people
Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline,
Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete
Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..
Bud differentiation is the physiology and morphological landmarks changed from nutrient growth to reproductive growth, directly influences opening for plant
Flower is as a result, therefore flower bud differentiation period has directly established the basis of fruiting period and harvest time to the nutritional supplementation of plant.In fruit jade
Often occur that bud differentiation is slow, and branch is few in rice planting process, causes inane fruit more, fruit is small.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the problems in existing fruit corn Process of Flower Bud Differentiation, provide one kind
Liquid fertilizer and preparation method thereof for fruit corn flower bud development.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of liquid fertilizer for fruit corn flower bud development, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation, multiple 1~10 part of phthalein nucleic acid, 0.01~3 part of chitosan oligosaccharide, urea 52~65
Part, 1~8 part of ammonium hydrogencarbonate, 8~32 parts of amino acid 1,64~78 parts of potassium sulfate, 2~12 parts of boric acid, calcium superphosphate 35~48, Portugal
0.1~5 part of grape Calciofon, 0.1~5 part of white vitriol；Total nitrogen content >=2.5mg/g, ammonia nitrogen contain in the liquid fertilizer
Amount >=0.4mg/g, the content of organic matter are 120~165g/L；
The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain
The culture medium of " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide
State city martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
Preferably, the liquid fertilizer is by following material composition: 1000 parts of active liquid fertilizer substrate preparation, multiple phthalein nucleic acid 3
~6 parts, 0.05~1 part of chitosan oligosaccharide, 55~60 parts of urea, 3~6 parts of ammonium hydrogencarbonate, 23~28 parts of amino acid, potassium sulfate 70~75
Part, 5~8 parts of boric acid, calcium superphosphate 39~43,1~3 part of calcium gluconate, 0.5~3 part of white vitriol；The liquid fertilizer
Middle total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter are 120~140g/L.
Preferably, the liquid fertilizer is by following material composition: 1000 parts of active liquid fertilizer substrate preparation, multiple phthalein nucleic acid 4
Part, 0.1 part of chitosan oligosaccharide, 58 parts of urea, 5 parts of ammonium hydrogencarbonate, 25 parts of amino acid, 73 parts of potassium sulfate, 6 parts of boric acid, calcium superphosphate
41.5 parts, 2 parts of calcium gluconate, 1 part of white vitriol；Total nitrogen content >=2.5mg/g, amino nitrogen content in the liquid fertilizer
>=0.4mg/g, the content of organic matter are 125~130g/L.
Further, the ratio between N, P, K are 2~4:1:2~4, preferably 3:1:3 in the liquid fertilizer.
Further, the amino acid is glycine, in proline, lysine, tyrosine, phenylalanine, aspartic acid
One or more.
Further, the ratio between the boron, zinc, calcium are 3~5:1:10~40.
A kind of preparation method of the liquid fertilizer for fruit corn flower bud development, comprising the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past
After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, continuing fermentation 7~8 days at 20~28 DEG C
Afterwards, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. multiple phthalein nucleic acid, chitosan oligosaccharide, urea, carbon the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
Sour hydrogen ammonia, amino acid, potassium sulfate, boric acid, calcium superphosphate, calcium gluconate, white vitriol, obtain the liquid fertilizer.
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, deposit number are GDMCC No.:60152.
Further, the pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material
When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.
Preferably, its amino-acid nitrogen content >=4.5g/L after the active liquid fertilizer substrate preparation is stored 15 days.
Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is
165g:250L。
Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge,
Show there is condensed milk shape gloss；Spore Gram's staining is positive.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:
Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7
It, is made bacterial strain after activation；
Y2. strain inoculated is into fluid nutrient medium after taking step Y1 activation obtained, temperature is 25~30 DEG C, revolution is
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.
Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white
Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the following advantages:
Liquid fertilizer of the invention can effectively facilitate the bud differentiation of fruit corn, and be conducive to quality and the fruit of fruit
The nutrient growth of tree.
Liquid fertilizer fertilizer efficiency prepared by the present invention is lasting, can constantly supply fruit tree growth and result in annual growth period
It needs, bud differentiation quality can be strengthened, increase yield, improve quality.
Liquid fertilizer of the invention increases branch pole length and the rugosity all remarkable effects of fruit corn, length
31.4%, rugosity increases 57.9%.The liquid fertilizer increases one times to the flower number of fruit corn, achieves unexpected
Technical effect.However, the liquid fertilizer does not all play substantive work to the new-tip length and fruit ear flower number of black waxy corn
With.As it can be seen that liquid fertilizer of the invention has selectivity to the bud differentiation of different corns, the bud differentiation of fruit corn is made
With and strong plantlets and rootage have very significant effect, to the corn of different cultivars selectivity with higher.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
It supports.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)
1. experimental material and condition
(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1
It rises, pH 7.5.
Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar,
Water is settled to 1 liter, pH 7.5.
Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).
(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Equal operations carry out in superclean bench.
(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns
The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to
It is saved in case.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, on oscillation shaking table under the conditions of 220 revs/min, 25 DEG C
Culture 5 days.
(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.
(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most
Good surviving colonies access solid medium squamous subculture.
(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium
All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium
Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.
(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar
100g is settled to 1000mL, pH 7.5.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining
It is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, deposit number are GDMCC No.:60152, and preservation address is that the province of GuangZhou, China city martyr Road 100 is micro-
Biological five building, institute's laboratory building.
The embodiment 2 a kind of preparation and application of the liquid fertilizer for fruit corn flower bud development
A kind of preparation method of the liquid fertilizer for fruit corn flower bud development the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation；
S5. multiple 1 part of phthalein nucleic acid, chitosan oligosaccharide the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
0.01 part, 52 parts of urea, 1 part of ammonium hydrogencarbonate, 8 parts of amino acid 1, (8 parts of lysine, 2 parts of tyrosine, 4 parts of phenylalanine, asparagus fern
4 parts of propylhomoserin), 64 parts of potassium sulfate, 2 parts of boric acid, 35 parts of calcium superphosphate, 0.1 part of calcium gluconate, 0.6 part of white vitriol obtain
Liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter are
120g/L.Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017,
Deposit number is GDMCC No.:60152.
The embodiment 3 a kind of preparation and application of the liquid fertilizer for fruit corn flower bud development
A kind of preparation method of the liquid fertilizer for fruit corn flower bud development the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. multiple 3 parts of phthalein nucleic acid, chitosan oligosaccharide the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
0.05 part, 55 parts of urea, 3 parts of ammonium hydrogencarbonate, 23 parts of amino acid (10 parts of lysine, 3 parts of tyrosine, 4 parts of phenylalanine, asparagus fern
6 parts of propylhomoserin), 70 parts of potassium sulfate, 5 parts of boric acid, 39 parts of calcium superphosphate, 1 part of calcium gluconate, 0.8 part of white vitriol obtain liquid
Body fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, content of organic matter 123g/
L。
The embodiment 4 a kind of preparation and application of the liquid fertilizer for fruit corn flower bud development
A kind of preparation method of the liquid fertilizer for fruit corn flower bud development the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. multiple 4 parts of phthalein nucleic acid, chitosan oligosaccharide the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
0.1 part, 58 parts of urea, 5 parts of ammonium hydrogencarbonate, 25 parts of amino acid (10 parts of lysine, 3 parts of tyrosine, 4 parts of phenylalanine, asparagus fern ammonia
Acid 8 parts), 73 parts of potassium sulfate, 6 parts of boric acid, 41.5 parts of calcium superphosphate, 2 parts of calcium gluconate, 1 part of white vitriol obtain liquid
Fertilizer；；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, content of organic matter 128g/L.
The embodiment 5 a kind of preparation and application of the liquid fertilizer for fruit corn flower bud development
A kind of preparation method of the liquid fertilizer for fruit corn flower bud development the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. multiple 10 parts of phthalein nucleic acid, chitosan oligosaccharide the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
3 parts, 65 parts of urea, 8 parts of ammonium hydrogencarbonate, 32 parts of amino acid (15 parts of lysine, 3 parts of tyrosine, 4 parts of phenylalanine, aspartic acid
10 parts), 78 parts of potassium sulfate, 12 parts of boric acid, 48 parts of calcium superphosphate, 5 parts of calcium gluconate, 3 parts of white vitriol obtain Liquid Fertilizer
Material；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, content of organic matter 161g/L.
The comparative example 1 a kind of preparation and application of the liquid fertilizer for fruit corn flower bud development
A kind of preparation method of the liquid fertilizer for fruit corn flower bud development the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S4. 58 parts of urea, ammonium hydrogencarbonate 5 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
Part, 73 parts of potassium sulfate, 6 parts of boric acid, 41.5 parts of calcium superphosphate, 2 parts of calcium gluconate, 1 part of white vitriol.
The application effect appraisal of liquid fertilizer for fruit corn flower bud development:
Nutrient needed for liquid fertilizer can quickly supplement the growth of fruit rattan, promotes fruit corn bud differentiation.To verify this hair
The liquid fertilizer of the bright preparation actual effect that large-scale demonstration is applied on fruit corn, applicant's selection are enterprising in fruit corn
Experiment and demonstration is gone.
1 materials and methods
1.1 for examination place 1 be located in Zixing City fruit corn base, 12 mu of testing ground area, soil fertility level compared with
Uniformly.
It is located in Jianning city black waxy corn base for examination place 2,12 mu of testing ground area, soil fertility level is more equal
It is even.
1.2 for studying 1 fruit corn of object, rattan line-spacing 0.4m*0.8m, the growing way golden mean of the Confucian school and consistent.
For studying 2 black waxy corn of object, rattan line-spacing 0.4m*0.8m, the growing way golden mean of the Confucian school and consistent.
1.3 for examination fertilizer embodiment 2~5 made from fruit corn root development liquid fertilizer, product forms are liquid.
1.4 it is each for examination place carry out 3 test process:
1. experimental group: every month, which pours, after plantation applies once for trying fertilizer, 500mL/ mus each, dilutes 200 times.
2. control group (CK): per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing
①。
3. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied
The same processing of fertile measure is 1..
Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre
It for cell, is repeated 3 times, management is consistent.Each processing randomly selects 5 plants of fruit rattans, observes rattan body young sprout growing state, acquires average
Value calculates bud quantity,.
2 results and analysis
2.1 different disposals are to young sprout growth, the influence of bud rate and flower number
As shown in Table 1, after applying the liquid fertilizer, the new-tip length and rugosity of fruit corn be significantly higher than control group with
Comparative example group, length increases 31.4% compared to the control group, and rugosity increases 57.9%, and fruit ear flower number increases compared to the control group
100%, achieve unexpected technical effect.However, the liquid fertilizer does not all have the growth of flower bud of black waxy corn
To the effect of essence.As it can be seen that liquid fertilizer of the invention has selectivity to the bud differentiation of different corns, to fruit corn
Bud differentiation effect is the most significant.
The young sprout growth of the different corn different disposals of table 1, fruit ear flower number situation result
| Processing | Bar is long (m) | Bar rugosity (cm) | Fruit ear flower number/piece |
| Fruit corn experimental group | 2.01 | 3.0 | 10 |
| Fruit corn control group | 1.53 | 1.9 | 5 |
| Fruit corn comparative example group | 1.73 | 2.3 | 7 |
| Black waxy corn experimental group | 1.87 | 2.6 | 7 |
| Black waxy corn control group | 1.68 | 1.9 | 5 |
| Black waxy corn comparative example group | 1.79 | 2.4 | 6 |
The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc. should be included within scope of the invention.
Claims (8)
1. a kind of liquid fertilizer for fruit corn flower bud development, which is characterized in that the liquid fertilizer is by following substance group
At: 1000 parts of active liquid fertilizer substrate preparation, multiple 1 ~ 10 part of phthalein nucleic acid, 0.01 ~ 3 part of chitosan oligosaccharide, 52 ~ 65 parts of urea, bicarbonate
1 ~ 8 part of ammonia, 8 ~ 32 parts of amino acid 1,64 ~ 78 parts of potassium sulfate, 2 ~ 12 parts of boric acid, calcium superphosphate 35 ~ 48, calcium gluconate 0.1 ~ 5
Part, 0.1 ~ 5 part of white vitriol；Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=0.4mg/g are organic
Matter content is 120 ~ 165g/L；
The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain " TY-
The culture medium of 001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then into filtrate
After sugar is added, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
2. being used for the liquid fertilizer of fruit corn flower bud development according to claim 1, which is characterized in that the liquid fertilizer
By following material composition: 1000 parts of active liquid fertilizer substrate preparation, multiple 3 ~ 6 parts of phthalein nucleic acid, 0.05 ~ 1 part of chitosan oligosaccharide, urea 55 ~
60 parts, 3 ~ 6 parts of ammonium hydrogencarbonate, 23 ~ 28 parts of amino acid, 70 ~ 75 parts of potassium sulfate, 5 ~ 8 parts of boric acid, calcium superphosphate 39 ~ 43, glucose
1 ~ 3 part of sour calcium, 0.5 ~ 3 part of white vitriol；Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=
0.4mg/g, the content of organic matter are 120 ~ 140g/L.
3. being used for the liquid fertilizer of fruit corn flower bud development according to claim 1, which is characterized in that the liquid fertilizer
By following material composition: 1000 parts of active liquid fertilizer substrate preparation, multiple 4 parts of phthalein nucleic acid, 0.1 part of chitosan oligosaccharide, 58 parts of urea, carbonic acid
5 parts of hydrogen ammonia, 25 parts of amino acid, 73 parts of potassium sulfate, 6 parts of boric acid, 41.5 parts of calcium superphosphate, 2 parts of calcium gluconate, white vitriol
1 part；Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter are 125 ~ 130g/
L。
4. being used for the liquid fertilizer of fruit corn flower bud development according to claim 1, which is characterized in that the Liquid Fertilizer
The ratio between N, P, K are 2 ~ 4:1:2 ~ 4, preferably 3:1:3 in material.
5. being used for the liquid fertilizer of fruit corn flower bud development according to claim 1, which is characterized in that the amino acid is
One or more of glycine, proline, lysine, tyrosine, phenylalanine, aspartic acid.
6. being used for the liquid fertilizer of fruit corn flower bud development according to claim 1, which is characterized in that the liquid fertilizer
The ratio between middle boron, zinc, calcium are 3 ~ 5:1:10 ~ 40.
7. a kind of preparation method of the liquid fertilizer for fruit corn flower bud development, which comprises the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20 ~ 28 DEG C after continuing fermentation 7 ~ 8 days, obtain
To fermentation liquid；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. multiple phthalein nucleic acid, chitosan oligosaccharide, urea, carbonic acid the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
Hydrogen ammonia, amino acid, potassium sulfate, boric acid, calcium superphosphate, calcium gluconate, white vitriol obtain the liquid fertilizer.
8. being used for the liquid fertilizer of fruit corn flower bud development according to claim 1, which is characterized in that the active liquid
Its amino-acid nitrogen content >=4.5g/L after fertile substrate preparation is stored 15 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113354474A (en) * | 2021-07-14 | 2021-09-07 | 新疆格莱克农牧科技有限公司 | Agricultural and forestry crop foliar bacterial liquid and preparation method thereof |
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