CN109939268A - A kind of antiseptic anti-adhesion sticking patch and the preparation method and application thereof - Google Patents
A kind of antiseptic anti-adhesion sticking patch and the preparation method and application thereof Download PDFInfo
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- CN109939268A CN109939268A CN201910216896.XA CN201910216896A CN109939268A CN 109939268 A CN109939268 A CN 109939268A CN 201910216896 A CN201910216896 A CN 201910216896A CN 109939268 A CN109939268 A CN 109939268A
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Abstract
The present invention relates to medical material technical field, a kind of antiseptic anti-adhesion sticking patch and the preparation method and application thereof is disclosed, the antiseptic anti-adhesion sticking patch includes polypropylene mesh and the poly- tea polyphenols film and nano-Ag particles for being coated on polypropylene mesh surface.Antiseptic anti-adhesion sticking patch of the present invention has both excellent antibacterial and preventing adhesiving effect, and raw material inexpensively easily obtains, and can reduce related complication after hernia mesh repair, improves the success rate and clinical efficacy of the repairing of hernia sticking patch, has a good application prospect.Preparation method is simple for the antiseptic anti-adhesion sticking patch, practical.
Description
Technical field
The present invention relates to medical material technical fields, more particularly, to a kind of antiseptic anti-adhesion sticking patch and its preparation side
Method and application.
Background technique
Hernia is the common disease of department of general surgery, and concept is that tissue, the organ in human body normal chamber gap pass through human body weakness
Position and leave normal anatomical position, can lead to the complication such as change, incarceration, the ischemic necrosis of organ dysfunction, in time
Effective clinical intervention is the important means preventing complication and occurring.In China, newly-increased hernia number of the infected is no less than 3,500,000 every year
Example, more than half patient need to take operative treatment, and China at least needs 1,800,000 hernia patching materials every year.It is clinically right at present
In the treatment of such disease, surgical operation row hernia sticking patch is mainly taken to repair.At present the sticking patch that clinically uses mainly include with
Lower four major class: the first kind is the polyester patches of nonabsorable, propene polymer patch, expanded polytetrafluoroethylsealing sticking patch；Second class is can
Polyglycolic acid, the polylactic-co-glycolic acid of absorption；Third class is composite patch；4th class is cell free extracellular matrix benefit
Piece (AEM).And in the minimally-invasive treatment age, the therapeutic effect of laparoscopic hernia repair gradually gets the nod and praises highly.At the same time,
Requirement of the laparoscopic hernia repair for hernia sticking patch still concentrates on good stretching tensile property, biocompatibility, anti-infective
And it is anti-be adhered, to prevent to occur after laparoscopic hernia repair the infection of art area, the complication such as intestinal obstruction caused by abdominal cavity is adhered, in turn
Lead to the repairing failure of hernia sticking patch.And it is anti-infective and it is anti-be adhered in two performances, it is single although there are the type on current market
Functional product, expensive, effect is inaccurate, and clinical application and popularization degree be not high.Thus, it is intended that research and develop and a kind of prepare letter
Single, price economy takes into account anti-infective, the anti-hernia sticking patch being adhered, meets clinical demand.
Summary of the invention
The present invention is directed to overcome at least one defect of the above-mentioned prior art, a kind of antiseptic anti-adhesion sticking patch is provided, it is described
Sticking patch has both excellent antibacterial and preventing adhesiving effect, and raw material inexpensively easily obtains, and correlation is concurrent after can reduce hernia mesh repair
Disease improves the success rate and clinical efficacy of the repairing of hernia sticking patch, has a good application prospect.
Another object of the present invention is to provide the preparation method of the antiseptic anti-adhesion sticking patch, the preparation method is simple
It is easy, it is practical.
The technical solution adopted by the present invention is that:
A kind of antiseptic anti-adhesion sticking patch, including polypropylene mesh and be coated on polypropylene mesh surface poly- tea polyphenols film and
Nano-Ag particles.
Tea polyphenols are a kind of green plants extracts, and performance safety has been confirmed in the safety of people's vivo applications
Approve with extensive, tea polyphenols have similar catechol structure with dopamine, can be formed on the surface of the material similar poly- more
The nanometer film of bar amine, which is to stablize attachment by complicated physics chemical action to be coated on polypropylene material surface, raw
Object safety is good, and property is stablized, and has good hydrophily and biocompatibility, polypropylene material is made to be not directly contacted with abdominal cavity
The complication such as the intestinal adhesion as caused by Polypropylene contact abdominal viscera can be greatly reduced in internal organs, meanwhile, tea polyphenols are also equipped with
Certain anti-microbial property can preferably prevent and treat the In vivo infection problem of implantation material, and the tea polyphenols prices of raw materials are low, and source is wide
It is general, belong to green renewable resource, there is huge application prospect.Nano silver is the metallic silver simple substance of Nano Particle, nanometer
Silver has strong inhibition and killing effect to the tens of kinds of pathogenic microorganisms such as Escherichia coli, gonococcus, chlamydia trachomatis, and
Drug resistance will not be generated, nano silver can kill 650 various bacterias in several minutes, and there is broad spectrum antibacterial and strong bactericidal to make
With, and durable antibacterial effect, it may additionally facilitate wound healing, promote the reparation and regeneration of damaged cell.The present invention passes through poly- third
Alkene mesh sheet surface coats poly- tea polyphenols film and nano silver obtains a kind of new antiseptic anti-adhesion sticking patch, keeps polypropylene material not direct
Abdominal viscera is contacted, can reduce the complication such as the intestinal adhesion as caused by Polypropylene contact abdominal viscera, while nano silver energy
Enough stabilizations are covered on polypropylene bodies surface, and are less prone to decomposition, and nano silver constituent concentration is low, and rate of release is slow, more with tea
Phenol all has good biocompatibility, and asepsis environment-protecting plays excellent antibacterial action so that sticking patch have excellent antibacterial and
Preventing adhesiving effect.
Preferably, the poly- tea polyphenols film with a thickness of 500nm~1.5 μm.It is highly preferred that the thickness of the poly- tea polyphenols film
Degree is 300nm~1.2 μm.
Preferably, the nano-Ag particles are adsorbed in poly- tea polyphenols film surface or poly- tea polyphenols film.
The polypropylene mesh is medical light weight sticking patch made of polypropylene woven, this polypropylene mesh can be not from after buying
It is directly used through any processing.Preferably, the mesh diameter of the polypropylene mesh is 0.1mm-1cm.Select mesh diameter
For the polypropylene mesh of 0.1mm-1cm, that repairing can be made in prepared peritonaeum to be maintained with polypropylene/tea polyphenols sticking patch is certain
It is mesh-structured, it is grown into conducive to tissue, the free entry and exit of macrophage and leucocyte, so as to make polypropylene/tea polyphenols sticking patch tool
There are preferable tissue intensity, pull resistance and anti-infection property.
The preparation method of the antiseptic anti-adhesion sticking patch, includes the following steps:
S1,0.3~1g tea polyphenols are weighed, is added in the hydrochloric acid that 45~55mL concentration is 1.5~2.5mol/L, is then added
25~35ml DMSO solution；
S2, polypropylene mesh is immersed in the solution of step S1 configuration, is heated to 65~75 DEG C, magnetic stirs 40~60h, reaction
After washing and room temperature dry to obtain polypropylene/tea polyphenols sticking patch.
Neutral reaction condition is maintained under S3, normal temperature state, and polypropylene/tea polyphenols sticking patch is immersed in 25~35mmol/L's
AgNO312h or more in solution continues magnetic and stirs to get antiseptic anti-adhesion sticking patch.
Application of the antiseptic anti-adhesion sticking patch in herniorrhaphy.Polypropylene in antiseptic anti-adhesion sticking patch of the present invention
With good tensile strength, chemical species aggressivity, foreign body sensation is light, and body fibroblast can be promoted to grow into, cladding
Then there is good antibiotic property, hydrophily and biocompatibility, and performance safety in the poly- tea polyphenols of polypropylene surface, it both can be with
The In vivo infection problem of prevention and treatment implantation material, and significantly reduce the intestinal adhesion as caused by Polypropylene contact abdominal viscera etc. simultaneously
Disease is sent out, and almost without security risk, nano silver has excellent antibiotic property, further improves the antibacterial effect of sticking patch, it can
Applied to herniorrhaphy.
Compared with prior art, the invention has the benefit that
(1) repairing polypropylene/tea polyphenols sticking patch contains a strata tea polyphenols film, tea polyphenols in peritonaeum of the present invention
Cheap as green plants extract, biological safety is good, and property is stablized, and has good hydrophily and bio-compatible
Property, stablizing attachment by complicated physics chemical action is coated on polypropylene material surface, forms a layers of polymer nanometer film, makes
Polypropylene material is not directly contacted with abdominal viscera, reduces the complication such as intestinal adhesion caused by being said by Polypropylene contact abdominal viscera,
Also the incidences such as intestinal obstruction can be reduced, and the tea polyphenols prices of raw materials are low, it is from a wealth of sources, belong to green renewable resource, has
Huge application prospect；Polypropylene has good tensile strength, chemical species aggressivity, and foreign body sensation is light, can promote machine
Body fibroblast grows into；Nano silver can kill 650 various bacterias in several minutes, and there is broad spectrum antibacterial and strong bactericidal to make
With, and durable antibacterial effect, it may additionally facilitate wound healing, promote the reparation of damaged cell and regeneration that there is excellent antibacterial to imitate
Fruit, and drug resistance will not be generated；
(2) antiseptic anti-adhesion sticking patch of the present invention there remains certain mesh-structured, grows into conducive to tissue, is huge
The free entry and exit of phagocyte and leucocyte has preferable tissue intensity, pull resistance and anti-infection property, is not easy bending and extruding
Deformation has excellent support power, convenient for fixing；
(3) during preparing the antiseptic anti-adhesion sticking patch, inorganic group (Ag+) can pass through specific reaction condition
It polymerize with tea polyphenols, forms tea polyphenol nano silver film, be covered on propene polymer patch surface.Nano silver (Ag
Nanopaticles, AgNPs) there is broad-spectrum antibacterial action, sustainedly and stably discharge process, low cytotoxicity and be not susceptible to
The characteristics such as drug resistance and be applied in the research and development of antimicrobial medical material, can be for antiseptic anti-adhesion sticking patch of the present invention
Antibacterial efficacy provides guarantee, preferably the In vivo infection problem of prevention and treatment implantation material, reduces the infection in the area herniorrhaphy Hou Shu, drop
The probability of low hernia sticking patch graft failure；
(4) manufacture craft of present invention preparation antiseptic anti-adhesion sticking patch is simple, at low cost, and property is stablized.
Detailed description of the invention
Fig. 1 is that the SEM of 1 polypropylene mesh of the embodiment of the present invention schemes.
Fig. 2 is 1 polypropylene of the embodiment of the present invention/tea polyphenols SEM figure.
Fig. 3 is that the SEM of 1 antiseptic anti-adhesion sticking patch of the embodiment of the present invention schemes.
Fig. 4 is the state diagram of bacterium solution painting on a lbmc agar plate in No. 1 24 hole porous plates.
Fig. 5 is that 6 polypropylene of embodiment/tea polyphenols sticking patch and antiseptic anti-adhesion sticking patch are placed in the bacteriostasis on agar plate
Figure.
Fig. 6 is that 11 antiseptic anti-adhesion sticking patch of embodiment is implanted into the 10th day lab diagram after rat abdominal cavity.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention more comprehensible, below in conjunction with attached drawing and specific embodiment party
Formula, the present invention is further described in detail.It should be understood that the specific embodiments described herein are only to solve
The present invention is released, and the scope of protection of the present invention is not limited.
Polypropylene mesh used in following example 1-5 is medical light weight sticking patch made of polyacrylic fibres braiding, and size is about
For 2cm × 2cm, mesh diameter is about 2mm.
Embodiment 1
A kind of antiseptic anti-adhesion sticking patch, including polypropylene mesh and be coated on polypropylene mesh surface poly- tea polyphenols film and
Nano-Ag particles, preparation process are as follows:
S1,0.5g tea polyphenols are weighed, is added in the hydrochloric acid that 50mL concentration is 2mol/L, 30ml DMSO is then added
Solution；
S2, polypropylene mesh is immersed in the solution of step S1 configuration, heats 70 DEG C, magnetic stirs 48h, after reaction
It washes and room temperature dries to obtain polypropylene/tea polyphenols sticking patch；
Neutral reaction condition is maintained under S3, normal temperature state, and polypropylene/tea polyphenols sticking patch is immersed in 30mmol/L's
AgNO312h or more in solution continues magnetic and stirs to get antiseptic anti-adhesion sticking patch.
Embodiment 2
A kind of antiseptic anti-adhesion sticking patch, including polypropylene mesh and be coated on polypropylene mesh surface poly- tea polyphenols film and
Nano-Ag particles, preparation process are as follows:
S1,0.5g tea polyphenols are weighed, is added in the hydrochloric acid that 50mL concentration is 2.5mol/L, 35ml is then added
DMSO solution；
S2, polypropylene mesh is immersed in the solution of step S1 configuration, heats 75 DEG C, magnetic stirs 40h, after reaction
It washes and room temperature dries to obtain polypropylene/tea polyphenols sticking patch；
Neutral reaction condition is maintained under S3, normal temperature state, and polypropylene/tea polyphenols sticking patch is immersed in 25mmol/L's
AgNO312h or more in solution continues magnetic and stirs to get antiseptic anti-adhesion sticking patch.
Embodiment 3
A kind of antiseptic anti-adhesion sticking patch, including polypropylene mesh and be coated on polypropylene mesh surface poly- tea polyphenols film and
Nano-Ag particles, preparation process are as follows:
S1,0.5g tea polyphenols are weighed, is added in the hydrochloric acid that 50mL concentration is 1.5mol/L, 25ml is then added
DMSO solution；
S2, polypropylene mesh is immersed in the solution of step S1 configuration, heats 65 DEG C, magnetic stirs 60h, after reaction
It washes and room temperature dries to obtain polypropylene/tea polyphenols sticking patch；
Neutral reaction condition is maintained under S3, normal temperature state, and polypropylene/tea polyphenols sticking patch is immersed in 35mmol/L's
AgNO312h or more in solution continues magnetic and stirs to get antiseptic anti-adhesion sticking patch.
Embodiment 4
A kind of antiseptic anti-adhesion sticking patch, including polypropylene mesh and be coated on polypropylene mesh surface poly- tea polyphenols film and
Nano-Ag particles, preparation process are as follows:
S1,0.3g tea polyphenols are weighed, is added in the hydrochloric acid that 45mL concentration is 2mol/L, 30ml DMSO is then added
Solution；
S2, polypropylene mesh is immersed in the solution of step S1 configuration, heats 70 DEG C, magnetic stirs 48h, after reaction
It washes and room temperature dries to obtain polypropylene/tea polyphenols sticking patch；
Neutral reaction condition is maintained under S3, normal temperature state, and polypropylene/tea polyphenols sticking patch is immersed in 30mmol/L's
AgNO312h or more in solution continues magnetic and stirs to get antiseptic anti-adhesion sticking patch.
Embodiment 5
A kind of antiseptic anti-adhesion sticking patch, including polypropylene mesh and be coated on polypropylene mesh surface poly- tea polyphenols film and
Nano-Ag particles, preparation process are as follows:
S1,0.8g tea polyphenols are weighed, is added in the hydrochloric acid that 55mL concentration is 2mol/L, 30ml DMSO is then added
Solution；
S2, polypropylene mesh is immersed in the solution of step S1 configuration, heats 70 DEG C, magnetic stirs 48h, after reaction
It washes and room temperature dries to obtain polypropylene/tea polyphenols sticking patch；
Neutral reaction condition is maintained under S3, normal temperature state, and polypropylene/tea polyphenols sticking patch is immersed in 30mmol/L's
AgNO312h or more in solution continues magnetic and stirs to get antiseptic anti-adhesion sticking patch.
TEM test is carried out to the antiseptic anti-adhesion sticking patch of embodiment 1-5 preparation, measures and resists described in gained embodiment 1-5
It is 300nm~1.2 μm that bacterium, which prevents adhesion and is coated on the poly- tea polyphenols film thickness on polypropylene mesh surface in sticking patch,.
SEM test is carried out to the antiseptic anti-adhesion sticking patch of embodiment 1-5 preparation, it is observed that compared to virgin pp mesh sheet,
Prepared antiseptic anti-adhesion patch faces are coated with one layer of white films, and visible partial size is about the small of 20~70nm in white films
Microballoon is as shown in Figure 1 the SEM figure of polypropylene mesh described in embodiment 1, and Fig. 2 is polypropylene/tea polyphenols sticking patch described in embodiment
SEM figure, Fig. 3 show antiseptic anti-adhesion sticking patch described in embodiment 1 SEM figure.By Fig. 1, Fig. 2, Fig. 3 it is found that polypropylene
Mesh sheet is coated with tea polyphenols film and nano-Ag particles, and the antiseptic anti-adhesion sticking patch has successfully been made.
By the proportional arrangement escherichia coli bacterium solution of 1:1000,6 24 hole porous plates are taken, respectively label 1,2,3,4,5,6,
1mL bacterium solution is added in each orifice plate, antiseptic anti-adhesion sticking patch prepared by embodiment 1-5 is respectively placed in the hole marked as 1-5
In plate, and the bacterium solution not to be put into antiseptic anti-adhesion sticking patch (label 6) is as blank control experimental group.It is observed that after 24 hours,
Bacterium solution still clear in orifice plate marked as 1-5, bacterium solution occurs muddy in the porous plate marked as 6, takes 0.1mL respectively
Bacterium solution in 1-5 orifice plate applies on a lbmc agar plate, it is observed that being showed no bacterial growth.Fig. 4 show the bacterium solution in No. 1 orifice plate
Apply state diagram on a lbmc agar plate.
Embodiment 6-10
The polypropylene mesh that size is about 1.5cm × 1.5cm is selected, respectively by side described in embodiment 1-5 step S1, S2
Method prepares polypropylene/tea polyphenols sticking patch, mends by the preparation antiseptic anti-adhesion of method described in step S1, S2, S3 described in embodiment 1-5
Piece is denoted as embodiment 6-10 polypropylene/tea polyphenols sticking patch and embodiment 6-10 antiseptic anti-adhesion sticking patch respectively.By the ratio of 1:1000
Example configuration staphylococcus aureus bacterium solution, bacterium solution 0.1ml staphylococcus aureus bacterium solution is applied on a lbmc agar plate, and in agar plate
Upper polypropylene/tea polyphenols the sticking patch and antiseptic anti-adhesion sticking patch placed by above-described embodiment 6-10 preparation, observes its shape afterwards for 24 hours
State, it is observed that polypropylene/tea polyphenols sticking patch and antiseptic anti-adhesion sticking patch have certain fungistatic effect, but in antiseptic anti-adhesion
The surrounding of sticking patch forms a more obvious antibacterial ring size, and diameter is about 3cm.From the experiment it is found that polypropylene/tea polyphenols sticking patch and
Antiseptic anti-adhesion sticking patch all has fungistatic effect, but is attached to the antibacterial effect of the antiseptic anti-adhesion sticking patch of nano silver more preferably.
It is illustrated in figure 56 polypropylene of embodiment/tea polyphenols sticking patch and antiseptic anti-adhesion sticking patch is placed in bacteriostasis figure on agar plate.
Animal embodiment
Embodiment 11-15
The polypropylene mesh that size is about 2.5cm × 2.5cm is selected, according to step S1, S2, S3 institute described in embodiment 1-5
The method preparation antiseptic anti-adhesion sticking patch stated, is denoted as embodiment 11-15 antiseptic anti-adhesion sticking patch respectively, takes 5 to weigh about 250g-
300g female sd inbred rats carry out intraperitoneal injection of anesthesia to rat with 1% yellow Jackets, dosage 50ml/kg, when anesthesia induction
Between be 8min.Exposed Rats abdomen immediately cleans out one piece of hairless region 6cm × 6cm in abdomen center with scissors.Routine disinfection paving
Towel is about 3.5cm skin incision, slit space 4cm, cuts parallel rat trunk at rat two sides abdominal incision two with scalpel
The longitudinal axis then successively separates simultaneously each layer of hara kiri muscle, into abdominal cavity along notch.
The antiseptic anti-adhesion sticking patch ethane via epoxyethane disinfection that embodiment 11-15 is prepared, soft being put into is intraperitoneal,
The good suture in sticking patch quadrangle is fixed on the inside of stomach wall with absorbable suture, successively closes abdominal cavity.Subsequent observation rat spirit,
Diet situation opens abdominal cavity after 10 days, whether there is or not adhesion and adhesion severity for observation sticking patch.
Through experimental observation, after 10 days, 5 rat abdominal cavities are opened, abdominal cavity is showed no infection, no covering property hydrops and empyema,
Sticking patch is showed no obvious adhesion, and sticking patch edge visible Filamentous mesentery adhesion in part has no the abdominal visceras adhesion such as small intestine, sticking patch connects
The one side for touching abdominal viscera is substantially feasibility, and Fig. 6 is that repairing is big with polypropylene/tea polyphenols sticking patch implantation in 11 peritonaeum of embodiment
10th day lab diagram behind mouse abdominal cavity.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate technical solution of the present invention example, and
It is not the restriction to a specific embodiment of the invention.It is all made within the spirit and principle of claims of the present invention
Any modifications, equivalent replacements, and improvements etc., should all be included in the scope of protection of the claims of the present invention.
Claims (7)
1. a kind of antiseptic anti-adhesion sticking patch, which is characterized in that including polypropylene mesh and be coated on the poly- of polypropylene mesh surface
Tea polyphenols film and nano-Ag particles.
2. antiseptic anti-adhesion sticking patch according to claim 1, which is characterized in that the poly- tea polyphenols film with a thickness of
500nm~1.5 μm.
3. antiseptic anti-adhesion sticking patch according to claim 2, which is characterized in that the poly- tea polyphenols film with a thickness of
300nm~1.2 μm.
4. antiseptic anti-adhesion sticking patch according to claim 1, which is characterized in that it is more that the nano-Ag particles are adsorbed on poly- tea
In phenol film surface or poly- tea polyphenols film.
5. antiseptic anti-adhesion sticking patch according to claim 1, which is characterized in that the mesh diameter of the polypropylene mesh is
0.1mm~1cm.
6. the preparation method of antiseptic anti-adhesion sticking patch described in claim 1-5 any claim, which is characterized in that including such as
Lower step:
S1,0.3-1g tea polyphenols are weighed, is added in the hydrochloric acid that 45~55mL concentration is 1.5~2.5mol/L, is then added
25~35ml DMSO solution；
S2, polypropylene mesh is immersed in the solution of step S1 configuration, is heated to 65~75 DEG C, magnetic stirs 40~60h, reaction
After washing and room temperature dry to obtain polypropylene/tea polyphenols sticking patch.
Neutral reaction condition is maintained under S3, normal temperature state, and polypropylene/tea polyphenols sticking patch is immersed in the AgNO of 25~35mmol/L3
12h or more in solution continues magnetic and stirs to get antiseptic anti-adhesion sticking patch.
7. application of the antiseptic anti-adhesion sticking patch in herniorrhaphy described in claim 1-5 any claim.
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