CN109825439A - A kind of preparation method of compound green alga and the method for carrying out culture of Chinese mitten crab using the compound green alga - Google Patents
A kind of preparation method of compound green alga and the method for carrying out culture of Chinese mitten crab using the compound green alga Download PDFInfo
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- CN109825439A CN109825439A CN201910194395.6A CN201910194395A CN109825439A CN 109825439 A CN109825439 A CN 109825439A CN 201910194395 A CN201910194395 A CN 201910194395A CN 109825439 A CN109825439 A CN 109825439A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses a kind of preparation method of compound green alga and the method for carrying out culture of Chinese mitten crab using the compound green alga, the preparation method includes the following steps: for 100 parts by weight tap water to be aerated more than for 24 hours；Injection disinfection removal miscellaneous bacteria is carried out to container inner wall with medicinal alcohol；After the volatilization of the medicinal alcohol of container inner wall is clean, the tap water of 100 parts by weight through aeration dechlorination is added, pours into the mixing concentration algae seedling solution containing chlorella, scenedesmus, haematococcus and egg capsule algae of 5 parts by weight, the culture medium dry powder of 0.2 parts by weight is then added；Container is placed in outdoor area without shade, is put into the sterilized inflator pump for installing pneumatic head, is sufficiently aerated；After 4~5 days, whole water body is dark green, and the inner wall of container has bubble wall built-up, obtains the first generation algae solution of compound green alga.The content astaxanthin for the chlorella that compound green alga of the invention contains itself is very high, and the yield and quality of freshwater shrimp is helped to improve for river crab intercropping freshwater shrimp pond, greatly improves cultivation comprehensive benefit.
Description
Technical field
The present invention relates to the preparation technical field of compound green alga, in particular to the preparation method of a kind of compound green alga and
The method for carrying out culture of Chinese mitten crab using the compound green alga.
Background technique
River crab industry is the pillar industry of Jiangsu Province's agricultural, the quality of culture of Chinese mitten crab benefit be not only related to fishery increase income,
Farmer richness.However the whole province's culture of Chinese mitten crab technical level is irregular, small part raiser river crab yield per mu is up to 200kg,
And many raiser's yield only reach 80kg reluctantly.Can this mainly protect good grass in the process with culture of Chinese mitten crab, mix up water, change
It is related to provide excellent environment for river crab growth for bottom.Water plant vitality it is vigorous whether, the quality of pond water quality substrate and early period
Rich water effect quality is inseparable.Early period is cultivated, raiser mainly uses inorganic fertilizer or fermented biological organic fertilizer fertilizing water
Matter, for inorganic fertilizer based on urea, calcium phosphate, fertility is quick, but the duration is not grown.Organic fertilizer is high to temperature requirement, but temperature
The high oxygen for being easy excessively liquid manure, largely consuming in ponds are spent, are difficult to control.In recent years, many raisers use chlorella
Come water quality of increasing fertilizer, but the finished color bought in the market it is dark green, it is tacky, without sedimentation, can not visually identify its quality, market price
Lattice are expensive.And single chlorella has a single function, rich water effect can not show a candle to compound green alga.Therefore it needs to explore a kind of suitable
Aquaculture cost is not only effectively reduced in the preparation method of compound green alga for culture of Chinese mitten crab, improves culture efficiency, and will
Biology spread cultivation technology introduce one line of breeding production, lead culture fishery mentions shelves upgrading.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation sides of compound green alga that can be improved river crab yield
Method and the method for carrying out culture of Chinese mitten crab using the compound green alga
In order to solve the above-mentioned technical problem, the technical solution of the present invention is as follows:
A kind of preparation method of compound green alga, includes the following steps:
(a1) 100 parts by weight tap water are aerated more than for 24 hours, are sufficiently dechlorinated；
(a2) injection disinfection removal miscellaneous bacteria is carried out to container inner wall with medicinal alcohol；
(a3) it is carried out disinfection with medicinal alcohol to inflator pump and pneumatic head；
(a4) after the volatilization of the medicinal alcohol of container inner wall is clean, 100 through aeration dechlorination in the step (a1) are added
The tap water of parts by weight pours into the mixing concentration algae seedling solution containing chlorella, scenedesmus, haematococcus and egg capsule algae of 5 parts by weight,
Then the culture medium dry powder of 0.2 parts by weight is added；
(a5) container filled in the step (a4) is placed in outdoor area without shade, is put into the sterilized pneumatic head that installs
Inflator pump is sufficiently aerated, and vessel port is opened wide completely, and daily illumination is not less than 8h；
(a6) visually it can be seen that compound green alga wall built-up, color are in emerald green after 2~3 days, will be aerated after interrupting can be observed
Container bottom has sediment, in faint yellow；Inflator pump is continued starting into work, after 4~5 days, whole water body is dark green, holds
The inner wall of device has bubble wall built-up, obtains the first generation algae solution of compound green alga；
(a7) close inflator pump, take middle layer solution carry out sediments microscope inspection, can be observed in microscope a large amount of chlorellas,
Scenedesmus, haematococcus and egg capsule algae can determine whether to be successfully prepared.
Preferably, the medicinal alcohol is the medicinal alcohol that concentration is 75%.
Preferably, outdoor temperature is 20 DEG C~28 DEG C in the step (a5).
Preferably, the preparation method of the compound green alga further includes the step that spreads cultivation:
(a8) by the first generation algae solution of the compound green alga obtained in the step (a6) according to production actual needs according to
Culture medium dry powder, the first generation algae solution of compound green alga, pure water ratio 1:25:500 cultivated again, can be obtained multiple
The second generation algae solution of mould assembly green alga；
(a9) by the second generation algae solution of the compound green alga obtained in the step (a8) according to production actual needs according to
Culture medium dry powder, the second generation algae solution of compound green alga, pure water ratio 1:25:500 cultivated again, can be obtained multiple
The third generation algae solution of mould assembly green alga, and so on, the algae solution of the required number that spreads cultivation can be obtained.
Preferably, the number that spreads cultivation in the step (a9) is no more than 5 times.
A kind of compound green alga that the preparation method using the compound green alga is prepared carries out culture of Chinese mitten crab
Method, using the compound green alga, is used when culture of Chinese mitten crab is in 3~June of early stage according to crab water depth 1.2m per acre
2kg~5kg is measured, full pool spilling head opens simultaneously micro-pore oxygenation facility oxygenation；The frequency of splashing of the compound green alga is every 15
It is carried out 1 time.
A kind of compound green alga that the preparation method using the compound green alga is prepared carries out culture of Chinese mitten crab
Method, when culture of Chinese mitten crab is in 7~September of middle and later periods, using the compound green alga, according to crab water depth 1.2m per acre
Dosage 4kg~10kg, full pool spilling head open simultaneously micro-pore oxygenation facility oxygenation；The frequency of splashing of the compound green alga is every
It carries out 1 time within 7 days.
By adopting the above technical scheme, the content astaxanthin for the chlorella that compound green alga of the invention contains itself is very high,
The yield and quality that freshwater shrimp is helped to improve for river crab intercropping freshwater shrimp pond, greatly improves cultivation comprehensive benefit；The present invention
Compound green alga be also crab shrimp one of food, it is possible to reduce the investment of feed can reduce aquaculture cost, realize that abridged edition increases
Effect.
Specific embodiment
Specific embodiments of the present invention will be further explained below.It should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but and does not constitute a limitation of the invention.In addition, this hair disclosed below
Technical characteristic involved in bright each embodiment can be combined with each other as long as they do not conflict with each other.
Embodiment 1
A kind of preparation method of compound green alga, includes the following steps:
Step 1: the tap water of 100kg is aerated more than for 24 hours, is sufficiently dechlorinated；
Step 2: preparation capacity is 250kg white clear plastic barrel 1, and with 75% medicinal alcohol to the inner wall of bucket
Carry out injection disinfection removal miscellaneous bacteria；
Step 3: inflator pump and 75% medicinal alcohol of pneumatic head are sterilized；
Step 4: tap water of the 100kg through aeration dechlorination is added after alcohol volatilization in bucket wall is clean, pours into containing bead
Algae, scenedesmus, haematococcus, egg capsule algae mixing concentration algae seedling solution 5kg, are then added culture medium dry powder 0.2kg, the culture backbone
Powder is using the culture medium dry powder for being applicable in algae；
Step 5: being placed in outdoor area without shade for bucket, is put into the sterilized inflator pump for installing pneumatic head, is sufficiently aerated, bucket
Mouth opens wide completely, and outdoor adaptive temperature is at 20 DEG C~28 DEG C, and the too high or too low breeding for being all unfavorable for algae, daily illumination is not
Lower than 8h, preparation time can slightly extend when lower than 8h；
Step 6: meticulous inspection, visually it can be seen that compound green alga wall built-up, color, will be in aerations in emerald green after 2~3 days
There is sediment at observable bucket bottom of having no progeny, in faint yellow.Inflator pump is continued starting into work afterwards, after 4~5 days, whole water body is in deep
Four walls of green, bucket have bubble wall built-up；
Step 7: close inflator pump, take middle layer solution carry out sediments microscope inspection, in the visual field it can be seen that a large amount of chlorellas,
Scenedesmus, haematococcus, egg capsule algae can determine whether to be successfully prepared；
Step 8: by obtain compound green alga can according to production actual needs according to culture medium dry powder, algae solution, pure water ratio
Example 1:25:500 is cultivated again, and the total degree that spreads cultivation is no more than 5 times.
The mixing green alga of acquisition after culture goes to shady place in time, and needs to be finished in 15~20 days, otherwise effect
Weaken.
Embodiment 2
A method of culture of Chinese mitten crab being carried out using the compound green alga, is included the following steps:
When culture of Chinese mitten crab is in 3~June of early stage, using compound green alga of the invention, according to the depth of crab water per acre
1.2m dosage 2kg~5kg, full pool spilling head open simultaneously micro-pore oxygenation facility oxygenation, and frequency of splashing is to carry out 1 time for every 15 days；
When culture of Chinese mitten crab is in 7~September of middle and later periods, using compound green alga of the invention, according to crab water per acre
Deep 1.2m dosage 4kg~10kg, full pool spilling head open simultaneously micro-pore oxygenation facility oxygenation, and frequency of splashing is to carry out 1 in every 7 days
It is secondary.
Early application compound green alga of the invention is cultivated, amino acid Fertilizer water paste and glucose are aided with, is not swum only dynamic
The quick breeding of the animal of object and cladocera provides nutrition, constructs stable plankton community, improves water body fertility, is crab
Shrimp or other aquatic products provide sufficient open-mouthed bait, and algae can be maintained to balance each other, and inhibit brown alga, dinoflagellate etc. in water body
Harmful algae.Early stage chlorella enters water body, and photosynthesis enhancing can promote the growth of pond water plant, control blue mud tongue fur hair
It is raw, build the excellent environment of crustacean existence.
It cultivates middle and later periods, especially high temperature season and uses compound green alga of the invention, can help to increase the molten of water body
Oxygen is conducive to the degradation of residual bait and poisonous and harmful substance, reduces ammonia nitrogen and nitrite of water body etc., prevents the outburst of cyanobacteria,
Improve breeding environment.
Furthermore the content astaxanthin for the chlorella that compound green alga of the invention contains itself is very high, green for river crab intercropping
The shrimp ponds pool helps to improve the yield and quality of freshwater shrimp, greatly improves cultivation comprehensive benefit.Compound green alga is also crab shrimp
One of food, it is possible to reduce the investment of feed can reduce aquaculture cost, realize cost-saving synergistic.
Above the embodiments of the present invention are described in detail, but the present invention is not limited to described embodiments.It is right
For those skilled in the art, in the case where not departing from the principle of the invention and spirit, these embodiments are carried out more
Kind change, modification, replacement and modification, still fall in protection scope of the present invention.
Claims (7)
1. a kind of preparation method of compound green alga, characterized by the following steps:
(a1) 100 parts by weight tap water are aerated more than for 24 hours, are sufficiently dechlorinated；
(a2) injection disinfection removal miscellaneous bacteria is carried out to container inner wall with medicinal alcohol；
(a3) it is carried out disinfection with medicinal alcohol to inflator pump and pneumatic head；
(a4) after the volatilization of the medicinal alcohol of container inner wall is clean, 100 weight through aeration dechlorination in the step (a1) are added
Part tap water, pour into 5 parts by weight containing chlorella, scenedesmus, haematococcus and egg capsule algae mixing concentration algae seedling solution, then plus
Enter the culture medium dry powder of 0.2 parts by weight；
(a5) container filled in the step (a4) is placed in outdoor area without shade, is put into the sterilized inflation for installing pneumatic head
Pump is sufficiently aerated, and vessel port is opened wide completely, and daily illumination is not less than 8h；
(a6) visually it can be seen that compound green alga wall built-up, color are in emerald green after 2~3 days, will be aerated after interrupting can be observed container
Sediment is arranged at bottom, in faint yellow；Inflator pump is continued starting into work, after 4~5 days, whole water body is dark green, container it is interior
Wall has bubble wall built-up, obtains the first generation algae solution of compound green alga；
(a7) inflator pump is closed, takes middle layer solution to carry out sediments microscope inspection, a large amount of chlorellas, grid can be observed in microscope
Algae, haematococcus and egg capsule algae can determine whether to be successfully prepared.
2. the preparation method of compound green alga according to claim 1, it is characterised in that: the medicinal alcohol is that concentration is
75% medicinal alcohol.
3. the preparation method of compound green alga according to claim 1, it is characterised in that: outdoor temp in the step (a5)
Degree is 20 DEG C~28 DEG C.
4. the preparation method of compound green alga according to claim 1, it is characterised in that: the preparation of the compound green alga
Method further includes the step that spreads cultivation:
(a8) first generation algae solution of the compound green alga obtained in the step (a6) is actually needed according to production according to culture
Backbone powder, the first generation algae solution of compound green alga, pure water ratio 1:25:500 cultivated again, can be obtained compound green
The second generation algae solution of algae；
(a9) second generation algae solution of the compound green alga obtained in the step (a8) is actually needed according to production according to culture
Backbone powder, the second generation algae solution of compound green alga, pure water ratio 1:25:500 cultivated again, can be obtained compound green
The third generation algae solution of algae, and so on, the algae solution of the required number that spreads cultivation can be obtained.
5. the preparation method of compound green alga according to claim 4, it is characterised in that: spreading cultivation in the step (a9)
Number is no more than 5 times.
6. a kind of compound green alga that the preparation method using compound green alga as claimed in claims 1-5 is prepared carries out
The method of culture of Chinese mitten crab, it is characterised in that: when culture of Chinese mitten crab is in 3~June of early stage, using the compound green alga, press
According to crab water depth 1.2m dosage 2kg~5kg, full pool spilling head per acre, micro-pore oxygenation facility oxygenation is opened simultaneously；It is described compound green
The frequency of splashing of algae is to carry out 1 time for every 15 days.
7. a kind of compound green alga that the preparation method using compound green alga as claimed in claims 1-5 is prepared carries out
The method of culture of Chinese mitten crab, it is characterised in that: when culture of Chinese mitten crab is in 7~September of middle and later periods, using the compound green alga,
According to crab water depth 1.2m dosage 4kg~10kg, full pool spilling head per acre, micro-pore oxygenation facility oxygenation is opened simultaneously；It is described compound
The frequency of splashing of type green alga is to carry out 1 time for every 7 days.
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| CN201910194395.6A CN109825439A (en) | 2019-03-14 | 2019-03-14 | A kind of preparation method of compound green alga and the method for carrying out culture of Chinese mitten crab using the compound green alga |
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| CN201910194395.6A CN109825439A (en) | 2019-03-14 | 2019-03-14 | A kind of preparation method of compound green alga and the method for carrying out culture of Chinese mitten crab using the compound green alga |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467266A (en) * | 2019-08-21 | 2019-11-19 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect shrimp-cultivation pond soil water regulation of algae |
| CN112616592A (en) * | 2020-12-18 | 2021-04-09 | 南县泽水居农业有限公司 | Eel rice planting method |
-
2019
- 2019-03-14 CN CN201910194395.6A patent/CN109825439A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467266A (en) * | 2019-08-21 | 2019-11-19 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect shrimp-cultivation pond soil water regulation of algae |
| CN112616592A (en) * | 2020-12-18 | 2021-04-09 | 南县泽水居农业有限公司 | Eel rice planting method |
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