CN109699812A - Solid state fermentation produces feeding saccharomyces cerevisiae-lactobacillus plantarum product mix method - Google Patents
Solid state fermentation produces feeding saccharomyces cerevisiae-lactobacillus plantarum product mix method Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The present invention relates to technical field of microbial fermentation, it is desirable to provide a kind of solid state fermentation produces feeding saccharomyces cerevisiae-lactobacillus plantarum product mix method.It include: saccharomyces cerevisiae seed liquor culture；The seed liquor culture of lactobacillus plantarum；Saccharomyces cerevisiae aerobic solids fermented and cultured；And saccharomyces cerevisiae and lactobacillus plantarum hybrid solid ferment.In product produced by the present invention, after saccharomyces cerevisiae, lactobacillus plantarum and its metabolite are used as additive that feed is added simultaneously, simple thallus is compared, can more promote the health of livestock and poultry, to also improve the application advantage of mixed fermentation products of the present invention.The present invention is easy to get using defatted rice bran etc., cheap agricultural and sideline product is fermentation raw material, lactobacillus plantarum is produced while producing saccharomyces cerevisiae, and use unsterilised raw material mixed fermentation method, drying is not had to after fermentation, the production cost of product is significantly reduced, application of the product in the feeds such as livestock and poultry, aquatic products can be pushed.
Description
Technical field
The present invention relates to technical field of microbial fermentation, more particularly to a kind of mixing of saccharomyces cerevisiae and lactobacillus plantarum
The production method of microbial fermentation.
Background technique
Saccharomyces cerevisiae (Saccharomyces cercvisiae) is a kind of edible microorganism and common Tiny ecosystem
Preparation strain.Except including yeast cells and extracellular metabolite and autolysate in the fermentation culture medium of the bacterium, as peptide,
Organic acid, oligosaccharides, amino acid, flavour enhancing substance and aromatic substance etc., also comprising beneficial to growth of animals or poultry there are many being proven
" unknown growth factor ".Through the scientific research and production application up to decades it was verified that yeast fermentation culture medium can have
Effect ground adjusts the quantity and ratio of microorganism in animal intestinal tract, inhibits pathogen, substantially reduces harmful microorganism in animal intestinal tract
Quantity, improve its intestinal microflora, reduce diarrhea rate, and improve food conversion ratio and daily gain, improve animal health shape
State, to significantly improve the productivity level of raising animal.Therefore, before saccharomyces cerevisiae has a wide range of applications in feed industry
Scape.
The lactic acid bacteria of lactobacillus plantarum this kind has been shown to have the work for improving animal intestinal tract health and improving immunity
With the lactic acid and lactein generated can directly act on pathogenetic bacteria, it is considered to be a kind of ideal growth promotion, substitution
The beneficial microbe of antibiotic, has broad application prospects.
Summary of the invention
The technical problem to be solved by the present invention is to overcome deficiency in the prior art, provide a kind of solid state fermentation production feeding
With saccharomyces cerevisiae-lactobacillus plantarum product mix method.This method is that the agricultural and sideline product being easy to get with cheap, material is original
Material, is aided with reasonable zymotechnique, while producing saccharomyces cerevisiae, is produced at low cost lactobacillus plantarum.
In order to solve the technical problem, solution of the invention is:
A kind of solid state fermentation is provided and produces feeding saccharomyces cerevisiae-lactobacillus plantarum product mix method, including following steps
It is rapid:
(1) saccharomyces cerevisiae seed liquor culture
31015 slant strains of Wine brewing yeast strain are accessed in triangular flask seed culture medium, after 30 DEG C of static gas wave refrigerator 28h
As triangular flask seed；Triangular flask seed access liquid amount is no more than 25L Cattell tank (Cattell by 5% inoculum concentration by volume
Tank orthobaric volume is 30L) in seed culture medium, through 30 DEG C of static gas wave refrigerator 20h, obtain saccharomyces cerevisiae Cattell tank seed (i.e. solid
Fermentation seed liquor)；Seed culture medium in the triangular flask and Cattell tank is saccharification defatted rice bran culture medium；
(2) the seed liquor culture of lactobacillus plantarum
Lactobacillus plantarum strain 11016 is accessed in triangular flask seed culture medium, becomes triangular flask after 37 DEG C of culture 14h
Seed；Triangular flask seed access loading amount is to train in the blue lid bottle seed culture medium of 1L through 37 DEG C by 1% inoculum concentration by volume
12h is supported, lactobacillus plantarum solid fermentation seed liquor is obtained, is saved at 0~4 DEG C；Seed in the triangular flask and blue lid bottle
Culture medium is MRS fluid nutrient medium；
(3) saccharomyces cerevisiae aerobic solids fermented and cultured
The Cattell tank seed liquor in step (1) is taken to access in solid fermentation culture medium, access amount is every 100 kilograms of culture mediums
Access 3~5 liters of seed liquors；It is fitted into fermentation koji tray after mixing, fermentation materials are with a thickness of 3cm or so, under the conditions of 30 DEG C
In fermenting cellar culture 20h, saccharomyces cerevisiae aerobic solids fermentation training object is obtained；
(4) saccharomyces cerevisiae and the fermentation of lactobacillus plantarum hybrid solid
It takes in step (2) lactobacillus plantarum solid fermentation to access saccharomyces cerevisiae aerobic solids in step (3) with seed liquor to send out
Ferment train object in, access amount in step (3) for access Cattell tank seed liquor solid fermentation culture medium poidometer, every 100
Kilogram solid fermentation culture medium accesses 1~2 liter of seed liquor；Be packed into breathing bag after mixing, after sealing under the conditions of 30 DEG C after
Supervention ferment is for 24 hours；After the completion of heat-preservation fermentation, fermenting cellar is removed, breathing bag is kept to seal state, drying and processing is not necessarily to, as makes wine
Yeast-lactobacillus plantarum product mix.
It is that 300ml is added in triangular flask according to 500ml triangular flask loading amount in the step (1) and (2) in the present invention
Culture medium.
In the present invention, in the step (1), the saccharification defatted rice bran culture medium is prepared by following methods:
Defatted rice bran is crushed, is added in 60 DEG C of water of 6 times of weight；The ratio of 100 units is added in every gram of defatted rice bran
Carbohydrase is added, is saccharified in 58~60 DEG C of heat preservations；After lasting stirring hour, residue is removed with four layers of filtered through gauze, is used after boiling
Again with four layers of filtered through gauze once to get clear defatted rice bran saccharified liquid；Water is added to make defatted rice bran saccharified liquid volume and addition
Volume before carbohydrase is consistent；Then ammonium dihydrogen phosphate is added, adding proportion is to add in every liter of defatted rice bran saccharified liquid
Add 5g；It is filling after mixing, in 105 DEG C of sterilizing 20min to get saccharification defatted rice bran culture medium.
In the present invention, in the step (2), prepared in the following manner in the MRS fluid nutrient medium:
Each component is taken in the following proportions: glucose 20g/L, peptone 10g/L, beef extract 10g/L, yeast extract 5g/
L, dipotassium hydrogen phosphate 2g/L, dibasic ammonium citrate 2g/L, sodium acetate 5g/L, tween 1g/L, magnesium sulfate 0.58g/L, manganese sulfate
0.25g/L, surplus are water；Adjusting pH is 6.2, in 121 DEG C of sterilizing 15min to get to MRS fluid nutrient medium.
In the present invention, in the step (3), solid fermentation culture medium is formulated by following methods:
100: 1~2: 75~85 extracting degreasing rice brans, ammonium dihydrogen phosphate and tap water by weight, are added by every gram of defatted rice bran
The ratio of 150 enzyme-activity units is added to take carbohydrase；Carbohydrase is first dissolved in part of running water, then it is mixed with remaining component
It is even, solid fermentation culture medium is arrived without sterilizing.
Inventive principle description:
Saccharomyces cerevisiae and lactobacillus plantarum are first prepared into seed by the present invention respectively, then by a certain percentage and time interval
Successively inoculation carries out hybrid solid fermentation.Due to joined a certain amount of carbohydrase in defatted rice bran, carbohydrase can be by degreasing rice
Starch in chaff is decomposed, and the small-molecule substances such as the glucose that saccharomyces cerevisiae can utilize are generated.Saccharomyces cerevisiae just utilizes in this way
The nutriments such as glucose and ammonium dihydrogen phosphate carry out aerobic fermentation, yeast thallus constantly growth and breeding.When saccharomyces cerevisiae into
When while row growth and breeding, a large amount of small molecule metabolite can be generated, these metabolins can promote the life of lactobacillus plantarum
It is long；The self-dissolving due to aging etc. of some yeast, can generate the nitrogenous small molecule such as some amino acid in fermentation process
Organic matter, lactobacillus plantarum can utilize the small-molecule substances such as these amino acid and glucose.Carbohydrase in the fermentation medium
The metabolin and autolysate of glucose, yeast growth generation that starch-splitting generates, these are the efficient life of lactobacillus plantarum
Long breeding can continuously provide needed nutrient matter.Lactobacillus plantarum can generate lactic acid and lactic acid while growth and breeding
The substance of a large amount of useful animals intestinal healths such as rhzomorph.
Compared with prior art, the beneficial effects of the present invention are:
1, in product produced by the present invention, saccharomyces cerevisiae, lactobacillus plantarum and its metabolite add as additive simultaneously
After entering feed, simple thallus is compared, can more promote the health of livestock and poultry, to also improve the application of mixed fermentation products of the present invention
Advantage.
2, the present invention is easy to get using defatted rice bran etc., cheap agricultural and sideline product is fermentation raw material, while producing saccharomyces cerevisiae
Lactobacillus plantarum is produced, and uses unsterilised raw material mixed fermentation method, drying is not had to after fermentation, significantly reduces product
Production cost, application of the product in the feeds such as livestock and poultry, aquatic products can be pushed.
Specific embodiment
Saccharomyces cerevisiae (Saccharomycescercvisiae) bacterial strain 31015 and lactobacillus plantarum that the present invention uses
(Lactobacillus plantarum) bacterial strain 11016 belongs to the prior art, and applicant is to introduce from Chinese industrial microbial bacteria
Kind preservation administrative center (CICC), the public can also pass through other open approach and obtain.Because bacterial strain itself is not belonging to the present invention
Innovative content, therefore be no longer discussed in detail.
Below by specific embodiment, the present invention is described in further detail, but its summary of the invention is not by these
Appearance is limited.
Saccharification defatted rice bran culture medium used is prepared by following methods in each embodiment: taking certain amount degreasing rice
Chaff crushes, and adds 60 DEG C of water of 6 times of defatted rice bran weight, and carbohydrase is added, and the additive amount of every gram of defatted rice bran carbohydrase is
100 units are saccharified in 58~60 DEG C of heat preservations, are stirred continuously, and after 4 hours, with four layers of filtered through gauze, residue are removed, after boiling
With again with four layers of filtered through gauze once to get clarification defatted rice bran saccharified liquid, supply moisture, make defatted rice bran saccharification liquid measure with
Volume before addition carbohydrase is consistent, and adding ammonium dihydrogen phosphate makes content 5g/ in every liter of defatted rice bran saccharified liquid
L。
MRS fluid nutrient medium used in each embodiment, component include (g/L): glucose 20, peptone 10, beef leaching
Cream 10, yeast extract 5, dipotassium hydrogen phosphate 2, dibasic ammonium citrate 2, sodium acetate 5, tween 1, magnesium sulfate 0.58, manganese sulfate 0.25 are adjusted
PH is 6.2,121 DEG C of sterilizing 15min.
Embodiment 1
(1) the seed liquor culture of saccharomyces cerevisiae (Saccharomyces cercvisiae strain number CICC 31015):
105 DEG C after filling triangular flask and Cattell tank, 20 minutes are sterilized up to saccharification defatted rice bran triangular flask seed culture medium
And the seed culture medium of Cattell tank；Saccharomyces cerevisiae slant strains are accessed in triangle seed bottle culture medium, 500ml triangular flask culture
Base loading amount is 300ml, becomes triangular flask seed after 30 DEG C of static gas wave refrigerator 28h；5% inoculum concentration is by triangular flask kind by volume
Son access liquid amount is no more than in 25L Cattell tank seed culture medium, through 30 DEG C of static gas wave refrigerator 20h at saccharomyces cerevisiae Cattell tank kind
Son, that is, solid fermentation seed liquor, it is spare；
(2) the seed liquor culture of lactobacillus plantarum (Lactobacillus plantarum strain number CICC 11016):
Lactobacillus plantarum strain is accessed in triangular flask culture medium, 300ml culture medium, warp are packed into 500ml triangular flask
Become triangular flask seed after 37 DEG C of culture 14h.Triangular flask seed access loading amount is that the blue of 1L is covered by 1% inoculum concentration by volume
In bottle culture medium, become lactobacillus plantarum solid fermentation seed liquor after 37 DEG C of culture 12h, is saved in 0~4 DEG C, it is spare.On
The seed culture medium for stating triangular flask and blue lid bottle is all made of MRS fluid nutrient medium.
(3) saccharomyces cerevisiae aerobic solids fermented and cultured:
Step (1) Cattell tank seed liquor is taken, the amount access solid hair of 5 liters of seed liquors is accessed by every 100 kilograms of defatted rice brans
It in ferment culture medium, is uniformly mixed, is fitted into fermentation koji tray, fermentation materials are being sent out under room temperature with a thickness of 3cm or so, in 30 DEG C
Ferment room fermented and cultured 20h, it is spare.
Solid fermentation culture medium is obtained by following manner: extracting degreasing rice bran, ammonium dihydrogen phosphate, carbohydrase add tap water mixed
It closes, without sterilizing, at solid fermentation culture medium, each component content is respectively as follows: defatted rice bran in solid fermentation culture medium: di(2-ethylhexyl)phosphate
Hydrogen ammonium: tap water 100: 1: 75 ratios by weight are formulated；Carbohydrase additive amount is that every gram of defatted rice bran adds 150 enzyme activity
Unit, when use, first dissolve carbohydrase in water.
(4) saccharomyces cerevisiae and the fermentation of lactobacillus plantarum hybrid solid:
Step (3) saccharomyces cerevisiae aerobic solids fermentation training object is taken, before fermenting based on defatted rice bran weight, every 100 kilograms de-
The amount that rouge rice bran accesses 2 liters of step (2) lactobacillus plantarum seed liquors is mixed, and breathing bag is packed into after mixing, in 30 after sealing
DEG C continue fermentation under room temperature for 24 hours, after the completion of heat-preservation fermentation, removes fermenting cellar, breathing bag is kept to seal state, without drying
Processing, as product.
Embodiment 2
In the present embodiment step (3), extracting degreasing rice bran, ammonium dihydrogen phosphate, carbohydrase add tap water to mix, without sterilizing,
At solid fermentation culture medium, each component content is respectively as follows: defatted rice bran in solid fermentation culture medium: ammonium dihydrogen phosphate: tap water
100: 1.5: 85 ratios are formulated by weight；Carbohydrase additive amount is that every gram of defatted rice bran adds 150 enzyme-activity units, is used
When first carbohydrase is dissolved in water.Step (1) Cattell tank seed liquor is taken, accesses 3 liters of seed liquors by every 100 kilograms of defatted rice brans
Amount access solid fermentation culture medium in, be uniformly mixed, is fitted into ferment koji tray in, fermentation materials are with a thickness of 3cm or so, in 30 DEG C
It is spare under room temperature in fermenting cellar fermented and cultured 20h；
In step (4), step (3) saccharomyces cerevisiae aerobic solids fermentation training object is taken, before fermenting based on defatted rice bran weight, often
The amount that 100 kilograms of defatted rice brans access 1 liter of step (2) lactobacillus plantarum seed liquor is mixed, and breathing bag is packed into after mixing, is sealed
Continue fermentation under room temperature for 24 hours in 30 DEG C after mouthful, after the completion of heat-preservation fermentation, remove fermenting cellar, breathing bag is kept to seal state,
Without drying and processing, as product.
Remaining technique, step are the same as embodiment 1.
Embodiment 3
In the present embodiment step (3), extracting degreasing rice bran, ammonium dihydrogen phosphate, carbohydrase add tap water to mix, without sterilizing,
At solid fermentation culture medium, each component content is respectively as follows: defatted rice bran in solid fermentation culture medium: ammonium dihydrogen phosphate: tap water
100: 2: 80 ratios are formulated by weight；Carbohydrase additive amount is that every gram of defatted rice bran adds 150 enzyme-activity units, when use
First carbohydrase is dissolved in water.Step (1) Cattell tank seed liquor is taken, accesses 4 liters of seed liquors by every 100 kilograms of defatted rice brans
It in amount access solid fermentation culture medium, is uniformly mixed, is fitted into fermentation koji tray, fermentation materials are with a thickness of 3cm or so, in 30 DEG C of rooms
It is spare in fermenting cellar fermented and cultured 20h under the conditions of temperature；
In step (4), step (3) saccharomyces cerevisiae aerobic solids fermentation training object is taken, before fermenting based on defatted rice bran weight, often
The amount that 100 kilograms of defatted rice brans access 1.5 liters of step (2) lactobacillus plantarum seed liquors is mixed, and breathing bag is packed into after mixing,
Continue fermentation under room temperature for 24 hours in 30 DEG C after sealing, after the completion of heat-preservation fermentation, removes fermenting cellar, breathing bag is kept to seal shape
State is not necessarily to drying and processing, as product.
Remaining technique, step are the same as embodiment 1.
Application method:
Feeding saccharomyces cerevisiae-lactobacillus plantarum product mix that the present invention prepares is used as feed addictive, can
This product is added in feed according to 0.4~0.6% weight percent, is uniformly mixed.
Claims (5)
1. a kind of solid state fermentation produces feeding saccharomyces cerevisiae-lactobacillus plantarum product mix method, which is characterized in that including under
State step:
(1) saccharomyces cerevisiae seed liquor culture
31015 slant strains of Wine brewing yeast strain are accessed in triangular flask seed culture medium, are become after 30 DEG C of static gas wave refrigerator 28h
Triangular flask seed；Triangular flask seed access liquid amount is no more than the Cattell tank seed culture of 25L by 5% inoculum concentration by volume
In base, through 30 DEG C of static gas wave refrigerator 20h, saccharomyces cerevisiae Cattell tank seed is obtained；Seed culture in the triangular flask and Cattell tank
Base is saccharification defatted rice bran culture medium；
(2) the seed liquor culture of lactobacillus plantarum
Lactobacillus plantarum strain 11016 is accessed in triangular flask seed culture medium, becomes triangular flask kind after 37 DEG C of culture 14h
Son；Triangular flask seed access loading amount is to cultivate in the blue lid bottle seed culture medium of 1L through 37 DEG C by 1% inoculum concentration by volume
12h obtains lactobacillus plantarum solid fermentation seed liquor, saves at 0~4 DEG C；Seed training in the triangular flask and blue lid bottle
Feeding base is MRS fluid nutrient medium；
(3) saccharomyces cerevisiae aerobic solids fermented and cultured
The Cattell tank seed liquor in step (1) is taken to access in solid fermentation culture medium, access amount is the access of every 100 kilograms of culture mediums
3~5 liters of seed liquors；It is fitted into fermentation koji tray after mixing, fermentation materials are with a thickness of 3cm, in fermenting cellar under the conditions of 30 DEG C
20h is cultivated, saccharomyces cerevisiae aerobic solids fermentation training object is obtained；
(4) saccharomyces cerevisiae and the fermentation of lactobacillus plantarum hybrid solid
Lactobacillus plantarum solid fermentation is taken in step (2) to access saccharomyces cerevisiae aerobic solids fermentation training in step (3) with seed liquor
In object, access amount in step (3) for access Cattell tank seed liquor solid fermentation culture medium poidometer, every 100 kilograms
Solid fermentation culture medium accesses 1~2 liter of seed liquor；It is packed into breathing bag after mixing, after supervention under the conditions of 30 DEG C after sealing
Ferment is for 24 hours；After the completion of heat-preservation fermentation, fermenting cellar is removed, breathing bag is kept to seal state, is not necessarily to drying and processing, as saccharomyces cerevisiae-
Lactobacillus plantarum product mix.
2. bottled according to 500ml triangle the method according to claim 1, wherein in the step (1) and (2)
Amount is that culture medium is added in 300ml in triangular flask.
3. the method according to claim 1, wherein in the step (1), the saccharification defatted rice bran culture medium
It is prepared by following methods:
Defatted rice bran is crushed, is added in 60 DEG C of water of 6 times of weight；It is added in the ratio that every gram of defatted rice bran adds 100 units
Carbohydrase is saccharified in 58~60 DEG C of heat preservations；After lasting stirring hour, residues are removed with four layers of filtered through gauze, with using again after boiling
Four layers of filtered through gauze are once to get clear defatted rice bran saccharified liquid；Add water that defatted rice bran saccharified liquid volume and addition is made to be saccharified
Volume before enzyme is consistent；Then ammonium dihydrogen phosphate is added, adding proportion is to add 5g in every liter of defatted rice bran saccharified liquid；
It is filling after mixing, in 105 DEG C of sterilizing 20min to get saccharification defatted rice bran culture medium.
4. the method according to claim 1, wherein leading in the MRS fluid nutrient medium in the step (2)
Following manner is crossed to prepare:
Each component is taken in the following proportions: glucose 20g/L, peptone 10g/L, beef extract 10g/L, yeast extract 5g/L, phosphorus
Sour hydrogen dipotassium 2g/L, dibasic ammonium citrate 2g/L, sodium acetate 5g/L, tween 1g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L,
Surplus is water；Adjusting pH is 6.2, in 121 DEG C of sterilizing 15min to get to MRS fluid nutrient medium.
5. the method according to claim 1, wherein solid fermentation culture medium passes through following in the step (3)
Method is formulated:
100: 1~2: 75~85 extracting degreasing rice brans, ammonium dihydrogen phosphate and tap water by weight are added by every gram of defatted rice bran
The ratio of 150 enzyme-activity units takes carbohydrase；Carbohydrase is first dissolved in part of running water, then it is mixed with remaining component
It is even, solid fermentation culture medium is arrived without sterilizing.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112544787A (en) * | 2020-11-02 | 2021-03-26 | 浙江大学 | Method for fermenting paper mulberry compound feed by bacteria and enzyme in synergy mode and compound feed thereof |
| CN112646736A (en) * | 2020-12-15 | 2021-04-13 | 北京化工大学 | Method for improving ethanol tolerance of saccharomyces cerevisiae by lactobacillus plantarum in bioethanol fermentation |
| CN113907187A (en) * | 2021-11-01 | 2022-01-11 | 山东省烟台市农业科学研究院 | Compound microbial feed additive and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101708031A (en) * | 2009-12-07 | 2010-05-19 | 浙江省农业科学院 | Method for producing anti-stress growth promoting fodder additive |
| CN102488089A (en) * | 2011-11-18 | 2012-06-13 | 吉林省农业科学院 | Preparation method of microecological culture feed additive |
| CN102618609A (en) * | 2012-03-26 | 2012-08-01 | 郑州凯乐生物能有限公司 | Method for comprehensively utilizing degreased rice bran |
| WO2015180176A1 (en) * | 2014-05-26 | 2015-12-03 | 上海创博食品技术发展有限公司 | Protein feed raw material prepared from asian carps and preparation method for protein feed raw material |
| CN105941899A (en) * | 2016-04-27 | 2016-09-21 | 浙江省农业科学院 | Production method for compound micro-ecological preparation for weaned piglets |
| CN107853452A (en) * | 2017-11-13 | 2018-03-30 | 广西塔锡科技有限公司 | A kind of production method of additive for microbe feedstuff |
-
2019
- 2019-02-24 CN CN201910139300.0A patent/CN109699812A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101708031A (en) * | 2009-12-07 | 2010-05-19 | 浙江省农业科学院 | Method for producing anti-stress growth promoting fodder additive |
| CN102488089A (en) * | 2011-11-18 | 2012-06-13 | 吉林省农业科学院 | Preparation method of microecological culture feed additive |
| CN102618609A (en) * | 2012-03-26 | 2012-08-01 | 郑州凯乐生物能有限公司 | Method for comprehensively utilizing degreased rice bran |
| WO2015180176A1 (en) * | 2014-05-26 | 2015-12-03 | 上海创博食品技术发展有限公司 | Protein feed raw material prepared from asian carps and preparation method for protein feed raw material |
| CN105941899A (en) * | 2016-04-27 | 2016-09-21 | 浙江省农业科学院 | Production method for compound micro-ecological preparation for weaned piglets |
| CN107853452A (en) * | 2017-11-13 | 2018-03-30 | 广西塔锡科技有限公司 | A kind of production method of additive for microbe feedstuff |
Non-Patent Citations (1)
| Title |
|---|
| 侍宝路等: "混菌固态发酵豆渣生产酸化饲料工艺条件研究", 《饲料工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112544787A (en) * | 2020-11-02 | 2021-03-26 | 浙江大学 | Method for fermenting paper mulberry compound feed by bacteria and enzyme in synergy mode and compound feed thereof |
| CN112646736A (en) * | 2020-12-15 | 2021-04-13 | 北京化工大学 | Method for improving ethanol tolerance of saccharomyces cerevisiae by lactobacillus plantarum in bioethanol fermentation |
| CN113907187A (en) * | 2021-11-01 | 2022-01-11 | 山东省烟台市农业科学研究院 | Compound microbial feed additive and preparation method thereof |
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