CN109566312A - A kind of method of bletilla striata seeds direct sowing and seedling - Google Patents

Abstract

The present invention provides a kind of methods of bletilla striata seeds direct sowing and seedling, the described method comprises the following steps: [vernalization], which is transferred directly in vessel after sufficiently impregnating seed, carries out vernalization, and seedling in greenhouse is carried out after seed greening；[seedling in greenhouse] does seedbed using conventional vegetable planting greenhouse, mixes the bletilla striata seeds of vernalization greening with the turfy soil for sufficiently pulverizing sieving after conventional method site preparation, and in uniform broadcasting to greenhouse seedbed, routine observation simultaneously keeps seedbed wet until seedling.The present invention uses conventional agriculture equipment and farming method nursery, and step is simple, completely disengage plant Zu Jie culturing room, MS culture medium, various hormones, various complexity professional techniques, facilities and equipment and the reagent such as microelement；Germination time foreshortens to 5~7 days, 98% or more germination percentage.Nursery material, facility are easy to get and at low cost, are easier to be grasped by professional technician and common peasant and practice.

Description

A kind of method of bletilla striata seeds direct sowing and seedling

Technical field

The invention belongs to Chinese herbal medicine planting technology fields, and in particular to a kind of to cultivate kind using the live streaming of Chinese herbal medicine bletilla striata seeds The method for culturing seedlings of seedling.

Background technique

The Chinese traditional herbs bletilla striata is because of its seed tiny (about 0.5mm × 2mm) and without whole seed structure, natural germination percentage pole It is low, used throughout the year by excavation wild resource sale, resource wretched insufficiency and by as a kind of rare rare Chinese herbal medicine, for a long time by It is pursued to market, thus application value with higher.

The main factor for restricting bletilla striata large-scale planting at present is the breeding of bletilla striata seedling, and block is mainly taken in artificial propagation Stem division propagation and plant tissue culture seedlings-raising.20~40 yuan/jin of stem tuber division propagation seedling market price, price and is not easy to obtain It takes, and continuous cropping diseases easily occurs；Plant tissue culture seedlings-raising, the intensive degree of technology is high, energy consumption is high, investment is high, even field Interior professional technician is limited by fund and technology and is also not easy to realize.

At this stage some scholars also proposed using bletilla striata seeds cultivate seedling technological invention, but such invention from Plant tissue culture seedlings-raising method is evolved, and height relies on facilities and equipment relevant to Plant Tissue Breeding method, MS culture medium, nothing Bacterium operating technology, expensive antibiotic, complicated microelement etc. are not equipment, technology and the reagent being generally familiar with and grasp, are hindered The large-scale promotion and application of bletilla striata seeds direct sowing and seedling technologies.

The present invention is directed to the deficiency of the drawbacks of above method for culturing seedlings and existing bletilla striata seeds seedling-raising technique, has invented one kind Using conventional farming method and culture facility, more the bletilla striata seeds direct sowing and seedling method of practicability, overall simplification bletilla seed Sub- nursery program is more suitable for those skilled in the art's grasp, while also easily being learnt and being practiced by other plant personnels outside field.

Summary of the invention

In view of the deficienciess of the prior art, it is an object of the present invention to provide a kind of bletilla striata seeds live streamings of more brief and practical The method of nursery, to solve the problems mentioned in the above background technology.

To achieve the goals above, the present invention is realized by following improved technology scheme, is specifically included:

(1) bletilla striata fruit pod is dried rapidly in 1~2 day after early September acquisition, and directly stripping fruit pod collects seed, is then turned It moves in container with lid in the potassium dihydrogen phosphate for using 2g/L and impregnates seed 8~24 hours；

(2) after soaking seed, seed-soaking liquid is directly transferred to the flat rectangular or bulge for being lined with single layer filter paper together with seed Interior, addition vernalization solution is to container bottom is just covered, and shaking container makes seed be evenly distributed, constant temperature illumination vernalization culture 5 ~7 days to seed greening；

(3) seedling raising greenhouse uses rotary tillage method site preparation, sufficiently smashes and paves not ridging and field ditch, is spaced 1~2 meter of laying, one brick Block band, spreading sawdust is made into seedbed after weeding 2~3 times；

(4) bletilla striata seeds of vernalization greening are sufficiently mixed with the turfy soil for sufficiently pulverizing sieving, are uniformly sprinkling upon on greenhouse seedbed, It sprinkles profoundly water, routine observation simultaneously keeps 60~70% humidity of seedbed.

Beneficial effects of the present invention:

(1), the present invention completely disengages facilities and equipment relevant to plant tissue culture seedlings-raising method and technical method；It does not need sterile Tissue culture room, completely disengages MS culture medium and other culture mediums, and overall process is special without aseptic technique, culture medium preparation technique etc. The very strong operating technology of industry is easier to realize plantation and cost is lower；

(2), overall process of the present invention is without using expensive antibiotic, without preparing and spraying the trace element solution of complicated ingredient； The matrix that seed seedbed uses special composition to prepare without spreading, easy to operate, at low cost, manageability；

(3), the present invention carries out nursery using conventional farming method and conventional greenhouse facility, and vernalization seed was completed to sprout in 5~7 days Greening is sent out, germination percentage saves sowing quantity and substantially shorten growing-seedling period 98% or more；

(4), the present invention is easier to be received by professional technician and common peasant and uses conventional farming method and facility very Plantation is realized under low economic input.

Detailed description of the invention

Attached drawing 1, presprouting of seeds culture first day.

Attached drawing 2, presprouting of seeds culture the 6th day.

Specific embodiment

To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to Specific embodiment, the present invention is further explained.

Example 1.

1, seed is sprouted

(1) seed impregnates: bletilla striata fruit pod is divided after early September acquires in drying rapidly in 1~2 day on cement flooring, or In 30~40 DEG C of flash baking in xeothermic baking oven.It directly peels off fruit pod and collects seed, eliminate remaining fruit pod fragment.Afterwards with being transferred to It uses in container with lid and is impregnated seed 8~24 hours in the 2g/L potassium dihydrogen phosphate of 2~3 times of seed soaking amounts；

(2) seed culture: seed, which directly pours into seed-soaking liquid together with seed after impregnating, is lined with the rectangular of single layer filter paper or circle In shape Flat bottom container, pouring volume is that seed-soaking liquid just covers container bottom, and shaking container makes seed be evenly distributed.Room temperature 25~ 30 DEG C, common 10 watts of LED white light desk lamp uninterruptedly irradiates；Or 27 DEG C of temperature are controlled using 70 liters of illumination boxs, intensity of illumination 500~1500Lx uninterruptedly irradiates, vernalization culture 5~7 days to seed greening.

2, seedbed makes

(1) seedbed forms: conventional vegetable greenhouse uses rotary tillage method site preparation, soil is sufficiently smashed pave after not ridging and field ditch, It is spaced 1~2 meter of laying, one brick band, 1~2 meter of wide seedbed is made；

(2) seedbed weeding: soil moisture 50~70% in greenhouse is kept, in first 2 months 50% Simanex wettable powders of sprinkling of sowing seeds 100~300 times of (mass volume ratio) dilution closing weedings of agent are primary；In sowing seeds 41% glyphosate isopropyl amine is sprayed again within first 1 month 60~170 times of salt aqua (volume ratio) dilution weedings are primary；

(3) seedbed covers: being uniformly spread on seedbed, 1~3 centimetre of thickness, is sufficiently poured in sowing the previous day using common sawdust Water-wet is saturating, keeps humidity 60~70%.

3, seedbed nursery

(1) seed broadcasting: the bletilla striata seeds of every 1~2 gram of vernalization greening and 5~10 kilograms sufficiently pulverize and through 6~10 targets standards The turfy soil that steel sieve or bamboo are sieved through sieve is sufficiently mixed, then uniform broadcasting is on greenhouse seedbed；

(2) seedling management: routine observation seedbed humidity, and keep 60~70% humidity of seedbed.

Claims (10)

1. a kind of bletilla striata method for culturing seedlings, which is characterized in that described method includes following steps:

(1) it is dried rapidly after the acquisition of bletilla striata fruit pod, directly stripping fruit pod collects seed, and is transferred in container with lid and impregnates；

(2) after soaking seed, directly seed is transferred in Flat bottom container, addition vernalization solution is to just covering container bottom Portion, vernalization culture to seed greening；

(3) greenhouse uses conventional method site preparation, not ridging and field ditch, interval 1~2 meter of laying, one brick band, and weeding 2~3 times Spreading sawdust afterwards；

(4) bletilla striata seeds of vernalization greening are sufficiently mixed with the turfy soil for sufficiently pulverizing sieving, are uniformly sprinkling upon on greenhouse seedbed, It sprinkles profoundly water, routine observation keeps seedbed wet.

2. method for culturing seedlings according to claim 1, which is characterized in that as described in step (1) to dry method are as follows: harvesting Bletilla striata fruit pod, which was directly placed under sunlight in 1~2 day, dries or is placed in 30~40 DEG C of flash baking in xeothermic baking oven.

3. method for culturing seedlings according to claim 1, which is characterized in that seed-soaking method as described in step (1) are as follows: will dry Bletilla striata fruit pod directly peel off collect seed, be immersed in the 2 g/L potassium dihydrogen phosphates of 2~3 times of seed soaking amounts, impregnate 8~ 24 hours.

4. breeding method according to claim 1, which is characterized in that method for treating seeds described in step (2) are as follows: Place mat single layer filter paper in Flat bottom container directly pours into seed-soaking liquid on filter paper together with seed, until soaking filter paper and just covering Firmly container bottom.

5. breeding method according to claim 1, which is characterized in that vernalization condition described in step (2) are as follows: room temperature 25 ~30 DEG C, common 10 watts of LED white light desk lamp uninterruptedly irradiates；Or 27 DEG C of temperature are controlled using 70 liters of illumination boxs, illumination is strong 500~1500Lx of degree uninterruptedly irradiates.

6. according to claim 5 urge wants condition, it is characterised in that: the time of presprouting of seeds greening is 5~7 days.

7. breeding method according to claim 1, which is characterized in that site preparation herbicidal methods described in step (3) are as follows: in First 2 months are sowed seeds by the rotary tillage of greenhouse soil and sufficiently smashes leveling；Soil moisture 50~70% is kept, 50% Simanex of sprinkling is wettable Property pulvis 100~300 times of (mass volume ratio) dilutions closing weedings it is primary, in sowing seeds first 1 month, to spray 41% glyphosate again different 60~170 times of (volume ratio) the dilution weedings of propylamine salt aqua are primary.

8. breeding method according to claim 1, which is characterized in that sawdust spreading described in step (3) with a thickness of 1~ 3 centimetres.

9. breeding method according to claim 1, which is characterized in that the sieving sieve of turfy soil described in step (4) is 6 ~10 mesh standard steels sieve or bamboo sieve.

10. breeding method according to claim 1, which is characterized in that type of seeding described in step (4) are as follows: often put down Square rice seedbed is broadcasted sowing after evenly mixing using 1~2 gram of seed and 5~10 kilograms of sieving turfy soils, keep seedbed humidity be 60~ 70%。