CN109463238A - A kind of breeding method of rhizoma polygonati stem tuber - Google Patents
A kind of breeding method of rhizoma polygonati stem tuber Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention belongs to Chinese medicines to breed field, specifically related to a kind of breeding method of rhizoma polygonati stem tuber, fresh rhizoma polygonati seedling stem is broken into two with one's hands according to every two section stem, as a breeding seedling stem, dry the stickum of wound, then it is immersed in 3-7min in treatment fluid, the treatment fluid includes following component: gibberellin, 6-BA, S-Ethyl ethylthio sulfonate and captan, it then will treated seedling stem and culture substrate wall paving, sealing, the humidity for controlling culture substrate is 55-65%, temperature is 22-27 DEG C, the culture substrate includes following component: perlite, peat soil, decomposed bark fines and treatment fluid, this method can be reduced the step of breeding, shorten the breeding time, and obtained seedling quality is preferable, consistency is high, the output value is higher.
Description
Technical field
The invention belongs to Chinese medicines to breed field, and in particular to a kind of breeding method of rhizoma polygonati stem tuber.
Background technique
Rhizoma polygonati (scientific name: Polygonatum sibiricum), also known as: polygonatum sibiricum Redoute, yellow chicken dish, pen tube dish, claw ginseng,
Tendrilleaf solomonseal rhizome, achickenclaw ginseng.For HUANGJING ZANYU CAPSULE, rhizome it is horizontal walk, cylindric, tubercle expands.Impeller generator, stockless.Medical and edible dual purpose plant,
With tonifying spleen, the effect of moistening lung and producing body fluid.In China, rhizoma polygonati kind one shares 31 kinds, and the only Yunnan for entering " Chinese Pharmacopoeia " is yellow
Essence, polygonatum sibiricum Redoute and polygonatum cyrtonema.No matter the source one of which kind of rhizoma polygonati seedling stem, which is taken from the wild rhizoma polygonati that nature is grown, at present is done
Seedling stem besides does breeding material using the rhizoma polygonati bud head of plantation and continues enlarged reproduction.Naturally the wild rhizoma polygonati grown is not only
Provenance is limited, and kind and quality cannot be guaranteed, and all digs down as long as rhizoma polygonati to do seedling.Seedling stem plantation the with terminal bud
2 years seedling stem second years that with rudiment, can not have terminal bud can only have a small amount of rudiment, the rhizoma polygonati kind of general 20% not terminal bud
Stem even arrives all uncertain energy rudiment of third year.And the rhizoma polygonati bud head planted does breeding material by long-distance transport, packs
It not giving careful note to details in journey, will also result in the damage of rhizoma polygonati bud head, rhizoma polygonati seedling stem extruding injury also results in a large amount of seedling stems and goes rotten, thus
Rhizoma polygonati is caused to plant third year ability rudiment after second year not rudiment or plantation, what seedling stem was gone rotten even not can rudiment.
Therefore, it is necessary to take effective means, expanding propagation provides the reliable and stable provenance of root system, bulb, terminal bud, Cai Nengman
The provenance demand of sufficient large area plantation rhizoma polygonati, could after planting second year rudiment it is neat.
The mating system of rhizoma polygonati specifically includes that rhizomes breeding and seed growing, different mating system, with greater need for not
Same processing step and time, success rate can also have apparent difference.And for rhizomes breeding, mating system base
This is consistent, is all directly rhizomes to be taken to be bred, and is only different that treatment measures planting percent is multifarious, and seedling cost also hangs
It is very very big.Although tissue culture propagation coefficient is high, operation difficulty is big, and operating process is cumbersome, breeds at high cost.Any life is not added
The propagation of seed stock method of long hormone and fungicide is at low cost, but seedling stem fungal infection is serious, and seedling stem rudiment planting percent is very low.
The research of polygonatum cyrtonema tissue culture rapid propagating technology, Liu Hongmei etc., seed, volume 29, the 12nd phase, 2010 12
Month, have studied the influence that different hormones breeds rhizoma polygonati rhizome.Its method used for, by polygonatum cyrtonema band bud rhizome, from
It is dug out in soil, washes away soil.(1) band bud rhizome is cut with knife and impregnate 5min in dish washing liquid aqueous solution, then rushed with tap water
Wash clean；(2) surface is cleaned with 75% ethyl alcohol, then is rinsed well with tap water；(3) dish washing liquid water impregnates 5min, tap water punching
Wash 60min.(following steps are completed in superclean bench).(4) 75% ethyl alcohol 0.5m in sterile deionized waters rinse twice
2.5%, sodium hypochlorite 5min sterile deionized water rinses 2 times, and 2.5% sodium hypochlorite 5min sterile deionized water rinses 2 times
2.5% sodium hypochlorite 5min sterilizing, deionized water 5 times taking-up aseptic filter paper wipe dries, cuts wound necrosis part with knife
Prepare inoculation.By the rhizoma polygonati disinfected with sprout tuber stem be connected to MS be basic culture medium, choose various concentration 6-BA, NAA,
2,4-D exogenous hormone is matched, and best adventitious bud induction culture base is screened out from it.Then again with 6-BA, NAA, 2,4-D
Exogenous hormone match MS culture medium, obtain healthy and strong a large amount of rhizoma polygonati regrowths.Then it with MS for basic culture medium, adds not
With the exogenous hormone of 6-BA, 2,4-D, IBA of concentration.Sucrose, 0.5% agar, pH 5.8-6.0 of culture medium additional 3%,
Sterilize 20min at 121 DEG C.Condition of culture (24-26 DEG C) is cultivated under the conditions of 1600lx, continuous illumination 12h/d.It will take root
Rhizoma polygonati band bud rhizome opening hardening 3d, then take out and wash away agar with tap water, being planted to matrix, (perlite and vermiculite 21 are mixed
Close) in.1 nutrient solution is applied every 7d.In the above scheme, main process is that will sterilize with sprout tuber stem, then with multiple
The hormone of different formulations is catalyzed it, applies Solution culture method after then putting it into culture substrate.
In sterilization phase, preferable mode is alcohol wipe, sodium hypochlorite sterilizing.
In the induction period of adventitious bud, adventitious bud can be differentiated while proliferation only in the culture medium containing 2,4-D；
Though having differentiation in culture medium containing NAA but differentiation rate being low, and the adventitious bud that every piece of explant differentiates is few.So growth
Element 2,4-D ratio NAA is more suitable for inducing the differentiation of rhizoma polygonati adventitious bud, and the differentiation rate that concentration is 0.2mg/L is high.
In the proliferation and strong seedling culture of adventitious bud, high concentration 6-BA is conducive to the Proliferation, Differentiation of rhizoma polygonati.In growth potential, add
The growing way in the culture medium that auxin is 2,4-D is added to be better than the culture medium of addition NAA.GA3Mutually inhibit with 6-BA, while GA3Have
Make the effect that plant grows tall.The content of the proliferation 6-BA of rhizoma polygonati stem tuber should be not less than 1.0mg/L.
In culture of rootage, than 2,4-D, NAA are more suitable for inducing rhizoma polygonati unrooted seedling rooting IBA, meanwhile, it further demonstrates
In root media be added 6-BA rhizoma polygonati can be made without offspring bud while taking root also is grown.
In above-mentioned rhizoma polygonati stem tuber breeding, more seedling, each different stage are swashed using different in order to obtain
Element is handled, though the breeding quantity of stem tuber is high, program is cumbersome, the poor quality of seedling, easy sick insect pest, after plantation
Rhizoma polygonati slight of stature, the output value be not high.Operation in condition it is also possible that do not have in the laboratory that condition has and in intelligent Greenhouse
Place simply can not large area production.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of breeding method of rhizoma polygonati stem tuber, this method can be reduced breeding
Step shortens the breeding time, and obtained seedling quality is preferable, and consistency is high, and the output value is higher.The present invention takes full advantage of rhizoma polygonati
Breeding First Year root system, bulb, terminal bud, which are formed, does not need the biological characteristics of sunlight, can mass propgation rhizoma polygonati kind indoors
Stem, then by form complete root system, bulb, terminal bud rhizoma polygonati seedling be planted to crop field and produce, can be cultivated in short-term
A large amount of rhizoma polygonati seedlings with root system, bulb, terminal bud.Such plantation rhizoma polygonati seedling last year, second year terminal bud break up blade,
Subsequently into normal growth.
The contents of the present invention include the following steps, fresh rhizoma polygonati seedling stem are broken into two with one's hands according to every two section stem, as one
Seedling stem is bred, the stickum of wound is dried, is then immersed in 3-7min in treatment fluid, the treatment fluid includes such as the following group
Point: gibberellin, 6-BA, S-Ethyl ethylthio sulfonate and captan, then will treated seedling stem and culture substrate wall paving, seal, control
The humidity of culture substrate be 55-65%, temperature be 22-27 DEG C, the culture substrate includes following component: perlite, peat soil,
Decomposed bark fines and treatment fluid.
Preferably, before fresh rhizoma polygonati seedling stem being broken into two with one's hands according to every two section stem, first by rhizoma polygonati seedling stem diaphragm seal
It saves.
Preferably, in the treatment fluid, gibberellin concentration 5-7mg/L, 6-BA concentration is 0.6-0.8mg/L, and S-Ethyl ethylthio sulfonate is dense
Degree is 0.5g/L, and captan concentration is 1g/L.
Preferably, the perlite, peat soil, decomposed bark fines weight ratio be (0.8-1.2): (0.8-1.2):
(0.8-1.2), more preferably 1:1:1.
Preferably, the humidity of the culture substrate is that 55-60% (holds culture substrate agglomerating, webs do not drip but
For degree), temperature is 25 DEG C.
The growth hormone is GA3 and 6-BA, and the root system that relying on seedling stem itself has promotes to sprout new root, be swashed by growing
Element promotes seedling stem to form new bulb and terminal bud.
Before fresh rhizoma polygonati seedling stem is broken into two with one's hands according to every two section stem, rhizoma polygonati seedling stem is placed in small basket uses film first
It is sealed.Material is bred first is that necessary used as needed, the bag packaging other than small basket cannot be used when breeding material by taking, with
Exempt from sack accumulated damage stem tuber and terminal bud that rhizoma polygonati stem tuber has been packed when measuring breeding material greatly, second is that root system must save
It is intact, with diaphragm seal moisturizing, guarantee that rhizoma polygonati stem tuber and root system are fresh and alive.
It has been observed that rhizoma polygonati stem tuber, which sprouts sprouting, needs the time, fast second year sprouts sprouting, and slow even all will not for 3 years
Sprout sprouting.Therefore, root-promoting hormone is not used in entire rhizoma polygonati seedling stem reproductive process, is relied on the root system that stem tuber has been formed and is existed
Restoration ecosystem in short-term in suitable temperature and humidity environment.GA3 and 6BA, which is only used only, promotes sprouting to be formed, and not only impregnates seedling stem
During need using growth hormone, also containing the growth hormone for promoting sprouting to be formed inside culture substrate, so that seedling stem is logical
Growth hormone is absorbed while crossing root system and seedling stem absorption moisture, to quickly form new bulb and terminal bud.
1. the rhizoma polygonatis kind such as polygonatum kingianurn, polygonatum cyrtonema, polygonatum sibiricum Redoute belongs to shade plants, bulb growth only needs suitable
Suitable temperature, humidity, moisture and nutrition, does not need sunlight.This biological characteristics give the indoor seedling stem of these three rhizoma polygonati stem tubers
Stripping and slicing quickly breeds and has established material base.In the environment of constant temperature and humidity, under the action of growth hormone, rhizoma polygonati seedling stem stripping and slicing
15 days sprouting new roots are bred, start within 60 days to sprout seed bud, 100 day time, one piece of stripping and slicing seedling stem can form a new plant
Strain.
2. indoor rhizome cutting propagation effectively reduces direct sunlight bring surface temperature and increases to Huang by Experimental Comparison
The influence of smart seedling stem.Indoor breeding planting percent is generally 94%, up to 99.7%, and outdoor is the same as numerous under height above sea level rhizoma polygonati
It educates planting percent and averagely there was only 32%.It is main the reason is that the mildew and rot ratio after outdoor stripping and slicing is very high, rainfall excessively will cause kind
Stem goes rotten, and temperature is more than 27 degree or more the formation and growth that can also inhibit rhizoma polygonati seedling stem bulb and terminal bud.
It is grown under area shading environment 3. rhizoma polygonati is suitble to cool, high altitude localities rhizome cutting propagation survival rate is relatively high, low sea
Area's rhizome cutting propagation survival rate of rising sheer from level ground is low.The seedling stem planting percent that the fresh and alive seedling stem of stem tuber root system is not damaged is high, uses by long-distance transport
Bag packaging carries out that rhizome cutting propagation mycotic infection rate is high, and planting percent is very low again.So guaranteeing that rhizoma polygonati stem tuber is fresh and alive and root system is fresh
It is living, the room temperature, humidity and mould of rhizome cutting propagation are controlled, is whether seedling stem rhizome cutting propagation can successful key technology.
The invention has the advantages that the prior art when handling rhizoma polygonati seedling stem, is generally required to be the root with bud directly
Stem or terminal bud are adjusted using plant growth regulator after sterilizing (such as alcohol wipe) processing, promote its germination
Long root, although the root and bud of the stem tuber that this mode obtains are more, it is more fragile, practices seedling in the process and is transplanted to certainly
When in right boundary, whichever link is out of joint to be all easy to cause seedling withered death, along with operation link is many and diverse, Mei Yibu
Operation creates a great impression to seedling.Besides tissue cultures are high to environmental requirement, and practical operation feasibility difficulty is big.This hair
It is bright break seedling stem according to every two section stem into two with one's hands, it is immersed in the mixed liquor of plant growth regulator and bacteriostatic agent, is then moved
It plants in the culture medium containing above-mentioned mixed liquor, although each stem tuber only sprouts a bulb and terminal bud, seldom sprouts two
A or more than two bulbs and terminal bud, but its rooting rate greatly improves, each seedling stem forms complete root system, and
After obtained seedling transplanting to crop field or other matrix, resistance and seedling early growth strength are promoted more bright
It is aobvious.By comparative test, the possible reason of the above results several aspects just like under are generated.1,2 section stems are compared to only with single bud
Seedling stem, and the contact area of plant growth regulator is less, decreases plant growth regulator and crosses intermuscular needling to seedling stem
Swash, is more appropriate in terms of the regulation of plant growth regulator.2, S-Ethyl ethylthio sulfonate and captan belong to sterilization material, phase
For alcohol and other sterilization materials, reduce the injury to seedling stem.S-Ethyl ethylthio sulfonate and captan also have plant growth suitable
Work as stimulation, and growth hormone combines generated effect obviously more preferable, so this may be not only that institute is impregnated in mixing
Bring addition effect, and growth hormone are combined and be achieved a better balance in stimulation and inhibition.
Specific embodiment
Embodiment 1
1. drying wound immediately after the polygonatum cyrtonema with root, snowy peak leaflet polygonatum sibiricum Redoute or polygonatum kingianurn break into two section stems
Then discharge surface is immersed in addition GA3,6-BA, S-Ethyl ethylthio sulfonate and captan solution five minutes.Growth hormone GA3 concentration 5-
7mg/L, 6-BA concentration 0.6-0.8mg/L, S-Ethyl ethylthio sulfonate 0.5g/L (2000 times), captan 1g/L (1000 times).
2. perlite, peat soil, decomposed crushing bark (weight ratio 1:1:1) stir evenly, then and by GA3 5-
7mg/L, 6-BA 0.6-0.8mg/L, S-Ethyl ethylthio sulfonate 0.5g/L (2000 times), captan 1g/L (1000 times) concentration be watered it is molten
Liquid is mixed.Said medicine stirs after being watered followed by perlite, peat soil, decomposed crushing bark are stirred
Uniformly, stop stirring when water content reaches 60%, cover film 2 hours, after waiting the culture substrates uniform pickup moisture such as perlites
Start to sow.In the culture substrate, the mixture of the perlite, peat soil and decomposed bark fines is grown with being added to
The water of hormone GA3 5-7mg/L, 6-BA 0.6-0.8mg/L, S-Ethyl ethylthio sulfonate 0.5g/L (2000 times), captan 1g/L (1000 times)
Solution is sufficiently mixed stirring, and control moisture content in medium is 55-60%.Appearance index is to hold agglomerating, webs cannot but drip
For degree.
3. spreading film in plastic crate, film bottom burrows, to exclude excessive moisture.One layer of culture substrate, one layer of rhizoma polygonati
Seedling stem, culture substrate maintain humidity 55-60%, then use diaphragm seal code heap.
4. 25 ± 2 degree of temperature of control.
5. outside air humidity maintains 60%.
6. in the case where 25 ± 2 degree of constant temperature, rhizoma polygonati seedling stem 15 days original root system sprouting new roots, rhizome cutting propagation two months
Afterwards, seed bud starts sprout growth, is initially formed than more complete root system, bulb and terminal bud, after 100 days after 60-70 days
Band new root sprouting is colonized, and the next spring rhizoma polygonati seedling stem sprouts sprouting.
Embodiment 2
1. drying wound immediately after the polygonatum cyrtonema with root, snowy peak leaflet polygonatum sibiricum Redoute or polygonatum kingianurn break into two section stems
Then discharge surface is immersed in addition GA3,6-BA, S-Ethyl ethylthio sulfonate and captan solution five minutes.Growth hormone GA3 concentration 6mg/
L, 6-BA concentration 0.8mg/L, S-Ethyl ethylthio sulfonate concentration 0.5g/L (2000 times), captan concentration 1g/L (1000 times).
2. perlite, peat soil, decomposed crushing bark (weight ratio 0.8:1.2:1) stir evenly, then and by GA3
6mg/L, 6-BA 0.8mg/L, S-Ethyl ethylthio sulfonate 0.5g/L (2000 times), the solution that the concentration of captan 1g/L (1000 times) is watered mix
Close stirring.Said medicine stirs after being watered followed by perlite, peat soil, decomposed crushing bark are stirred
Even, water content stops stirring when reaching 60%, covers film 2 hours, opens after waiting the culture substrates uniform pickup moisture such as perlites
Begin to sow.In the culture substrate, the mixture of the perlite, peat soil and decomposed bark fines swashs with growth is added to
Plain GA3 6mg/L, 6-BA 0.8mg/L, S-Ethyl ethylthio sulfonate 0.5g/L (2000 times), the aqueous solution of captan 1g/L (1000 times) are abundant
It is mixed, control moisture content in medium is 55-60%.Appearance index is to hold agglomerating, webs cannot but drip for degree.
3. spreading film in plastic crate, film bottom burrows, to exclude excessive moisture.One layer of culture substrate, one layer of rhizoma polygonati
Seedling stem, culture substrate maintain humidity 55-60%, then use diaphragm seal code heap.
4. 25 ± 2 degree of temperature of control.
5. outside air humidity maintains 60%.
6. in the case where 25 ± 2 degree of constant temperature, rhizoma polygonati seedling stem 15 days original root system sprouting new roots, rhizome cutting propagation two months
Afterwards, seed bud starts sprout growth, is initially formed than more complete root system, bulb and terminal bud, after 100 days after 60-70 days
Band new root sprouting is colonized, and the next spring rhizoma polygonati seedling stem sprouts sprouting.
Embodiment 3
Embodiment 3 and embodiment 1 are distinguished as, the perlite, peat soil, the decomposed weight ratio crushed between bark
For 1.2:1.1:0.9.
Comparative example 1
Compared to embodiment 1, the difference of comparative example 1 is according to each section stem to break fresh rhizoma polygonati seedling stem into two with one's hands.It is other
With embodiment 1.
Comparative example 2
Compared to embodiment 1, the difference of comparative example 2 is, fresh rhizoma polygonati seedling stem is broken into two with one's hands according to every two section stem, as
One breeding seedling stem is first impregnated with S-Ethyl ethylthio sulfonate and captan solution, it is molten that the rhizoma polygonati seedling stem of immersion is then added to growth hormone
In liquid (GA3 and 6-BA).The other the same as in Example 1.
Comparative example 3
Compared to embodiment 1, the difference of comparative example 3 is, culture substrate includes following component: perlite, peat soil, decomposed
Bark fines do not include treatment fluid.The other the same as in Example 1.
Comparative example 4
Compared to embodiment 1, the difference of comparative example 4 is, the treatment fluid includes following component: growth hormone and alcohol.
The other the same as in Example 1.
The seedling stem rudiment situation of embodiment 1-3 and comparative example 1-4 is compared, main standard of comparison is when identical
Rooting rate, bulb and the terminal bud formation rate of interior stem tuber, the new fresh weight ratio for forming seedling stem and seedling stem, the mildew and rot rate of seedling stem, fresh goods produce
Amount, dry product dehydration rate (directly drying to obtain) and polyoses content, obtain following data.
Illustrate: in all data of the following example and comparative example, the data only compiled by taking polygonatum cyrtonema as an example.
The new formation bulb increment of snowy peak leaflet polygonatum sibiricum Redoute is 2.125 times that polygonatum cyrtonema newly forms bulb increment, polygonatum kingianurn
The new bulb increment that formed is 3.88 times that polygonatum cyrtonema newly forms bulb increment.Snowy peak leaflet polygonatum sibiricum Redoute bulb polysaccharide contains
Amount is generally in the section 15%-17%, and polygonatum kingianurn rhizoma polygonati bulb polyoses content is generally in the section 7%-8.3%, and items refer to
Mark is very regular, and repeats no more herein.Following table is polygonatum cyrtonema seedling stem breeding data, wherein seedling stem rooting rate, bulb
It is to sow 100 days data for carrying out statistic mixed-state with terminal bud formation rate, new bulb and the seedling stem fresh weight ratio of being formed is sowing current year 11
Month detection data, the correlation datas such as fresh goods yield, dry product dehydration rate, polyoses content, seedling plant height and seedling stem size be move
Plant the data detected November to crop field second year.
The bulb and product quality contrast table that 1 polygonatum cyrtonema distinct methods of table processing seedling stem obtains
After the dry product dehydration rate refers to that the fresh goods of same weight sufficiently removes moisture, obtained dry product weight is divided by fresh
The percentage of product weight.
Because be with root sowing seedling stem, as long as stem tuber and root system Preservation Treatment are proper, no matter embodiment or comparative example
New root rooting rate is generally very high, and from the point of view of dry product dehydration rate and Siberian solomonseal rhizome polysaccharide content, embodiment and comparative example difference is little, and two
Data are stable in a more constant section.From the point of view of bulb and terminal bud formation rate, embodiment and comparative example 1, comparative example 2
It is relatively high, and comparative example 3,4 bulb of comparative example and terminal bud formation rate only have 52.3% and 85.3%, 3 culture substrate of comparative example
In do not contain growth hormone, 4 culture medium of comparative example contains growth hormone, but alcohol still has certain stimulation to seedling stem.
Therefore the mildew and rot rate of seedling stem is relatively high with respect to embodiment after sowing, the mildew and rot rate 21.2% of the seedling stem of comparative example 3, the kind of comparative example 4
Stem goes rotten rate 7.63%.Above data can affirm that the growth hormone formation of corm new to seedling stem and terminal bud formation relationship are very big,
Fungal attack is controlled without the use of fungicide, the seedling stem that will cause sowing to a certain extent is gone rotten, so that seedling stem be made to lose life
Power.Comparative example 1 is because wound surface is excessive, and the mildew and rot rate of seedling stem increases for opposite embodiment, reaches 1.33%.Comparative example 3 is trained
It supports base and does not contain growth hormone and fungicide, there is that not sprout within continuous 3 years bulb and terminal bud existing after seedling stem wound healing unexpectedly
As.Therefore, during rhizoma polygonati rhizome cutting propagation, keep root system and stem tuber it is fresh and alive be guarantee stem tuber rhizome cutting propagation survival rate substance
Basis, and appropriate supplemented with exogenous hormone, stimulation seedling stem form new bulb and terminal bud, are to guarantee that seedling stem breeds successful technology
Key point.
The seedling quality that different stem tuber breeding methods obtains is preferably consistent, and the quality of the product harvested in this way just has guarantor
Barrier, the present invention compare the distribution of the plant height that different processing methods obtains and bulb size, for evaluating point of product quality
Cloth situation, is specifically shown in Table 2-3.
The seedling plant height contrast table (%) that 2 polygonatum cyrtonema distinct methods of table processing seedling stem obtains
The bulb size contrast table (%) that 3 polygonatum cyrtonema distinct methods of table processing stem tuber obtains
From the analysis of the data of table 2-3 it is found that growth (plant height and the ball of the application treated seedling that stem tuber breeds
Stem size) distribution situation more concentrates, and the distribution of concentration is more advantageous to the factorial production and breeding, improves the quality of product.And
And:
1. the processing of 1 seedling stem of comparative example is breaks into two with one's hands according to each section, because parent is with respect to embodiment 1, embodiment 2, implementation
Example 3 and comparative example 2, comparative example 3, comparative example 4 will be small, so the bulb formed is with respect to embodiment 1, embodiment 2, embodiment
3 and comparative example 2, comparative example 3, comparative example 4 will be small, the bulb newly formed with seedling stem fresh weight than only 0.412 times, obtained kind
Seedling mean height ratio embodiment 1, embodiment 2, embodiment 3 and comparative example 2, comparative example 4 will be short, and new formation bulb size will
It will be small than embodiment 1, embodiment 2, embodiment 3 and comparative example 2, comparative example 4.Seedling stem size and maternal nutrition number it is certain
Seedling height and the new size for forming bulb are determined in degree.
2. the processing of 3 seedling stem of comparative example does not have to growth hormone, without S-Ethyl ethylthio sulfonate and captan, the kind Stem nematode newly formed is weary
Power, sprouting sprout the section that the later plant strain growth of blade is highly concentrated in 26-30 centimetres, and the bulb size newly formed also all only collects
In in 1-2.6 centimetres of section.Growth hormone and fungicide have played key effect in rhizoma polygonati stem tuber rhizome cutting propagation.
3. polygonatum kingianurn, the various processing modes of bulb size of snowy peak leaflet polygonatum sibiricum Redoute are all than polygonatum cyrtonema bulb increment
Much larger, polygonatum kingianurn newly forms bulb formation bulb newer than polygonatum cyrtonema and is averaged 3.88 times big, the new shape of snowy peak leaflet polygonatum sibiricum Redoute
It is average 2.125 times big at bulb formation bulb newer than polygonatum cyrtonema.
4. the highest kind of Siberian solomonseal rhizome polysaccharide content is snowy peak leaflet polygonatum sibiricum Redoute, polyoses content is in 15%--17%, secondly
It is polygonatum cyrtonema, Siberian solomonseal rhizome polysaccharide content 12.02%--13.6%, the minimum kind of Siberian solomonseal rhizome polysaccharide content is polygonatum kingianurn, and rhizoma polygonati is more
Sugared content 7%-8.3% or so.
5. polygonatum cyrtonema plants First Year, it is 0.477 times that new formation bulb, which increases weight with seedling stem than average value, plants second year
Stem tuber mushrooms out after forming huge root mass, increases by 6.325 times of multiplying power.Therefore it can affirm, the growth of rhizoma polygonati stem tuber is with root
System forms in close relations, and therefore, during rhizome cutting propagation, the root system for protecting rhizoma polygonati seedling stem is particularly important.
Claims (10)
1. a kind of breeding method of rhizoma polygonati stem tuber, it is characterised in that: include the following steps, by fresh rhizoma polygonati seedling stem according to every two
A section stem is broken into two with one's hands, as a breeding seedling stem, is dried the stickum of wound, is then immersed in 3-7min in treatment fluid,
The treatment fluid includes following component: gibberellin, 6-BA, S-Ethyl ethylthio sulfonate and captan, then will treated seedling stem and culture medium
Matter wall paving, sealing, the humidity for controlling culture substrate is 55-65%, and temperature is 22-27 DEG C, and the culture substrate includes such as
Lower component: perlite, peat soil, decomposed bark fines and treatment fluid.
2. the breeding method of rhizoma polygonati seedling stem as described in claim 1, characterized in that by fresh rhizoma polygonati seedling stem according to every two
Before section stem is broken into two with one's hands, rhizoma polygonati seedling stem diaphragm seal is saved first.
3. the breeding method of rhizoma polygonati seedling stem as described in claim 1, characterized in that in the treatment fluid, gibberellin concentration 5-
7mg/L。
4. the breeding method of rhizoma polygonati seedling stem as described in claim 1, characterized in that in the treatment fluid, 6-BA concentration is
0.6-0.8mg/L。
5. the breeding method of rhizoma polygonati seedling stem as described in claim 1, characterized in that in the treatment fluid, S-Ethyl ethylthio sulfonate concentration is
0.5g/L。
6. the breeding method of rhizoma polygonati seedling stem as described in claim 1, characterized in that in the treatment fluid, captan concentration is
1g/L。
7. the breeding method of rhizoma polygonati seedling stem as claimed in any one of claims 1 to 6, characterized in that the perlite, peat soil,
The weight ratio of decomposed bark fines is (0.8-1.2): (0.8-1.2): (0.8-1.2).
8. the breeding method of rhizoma polygonati seedling stem as claimed in claim 7, characterized in that the perlite, peat soil, decomposed bark
The weight ratio of powder is 1:1:1.
9. the breeding method of rhizoma polygonati seedling stem as claimed in any one of claims 1 to 6, characterized in that the humidity of the culture substrate
For 55-60%.
10. the breeding method of rhizoma polygonati seedling stem as claimed in any one of claims 1 to 6, characterized in that the temperature of the culture substrate
Degree is 25 DEG C.
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