CN109394639A - A kind of anti-ageing activating essence and preparation method thereof containing ferment - Google Patents
A kind of anti-ageing activating essence and preparation method thereof containing ferment Download PDFInfo
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The invention discloses a kind of anti-ageing activating essence and preparation method thereof containing ferment.The anti-ageing activating essence containing ferment includes following components: allantoin, glycerol, hydrolecithin, caprylic/capric triglyceride, plant enzyme, high mountain rose indian azalea leaf stem cell culture extract, citronellol, EDTA- disodium, preservative and deionized water.The effect of anti-ageing activating essence contains there are many active skull cap components, can effectively penetrate into skin histology, mutually coordinated performances moisturizing water conservation, desalination wrinkle, anti-ageing, whitening spot-removing, at the same it is property stabilization, highly-safe, without any adverse reaction.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of anti-ageing activating essence and its preparation side containing ferment
Method.
Background technique
Skin aging is the skin aging phenomenon as caused by natural cause or non-natural factor.Skin histology day after people's birth
It reaches increasingly, function is gradually active.The growth stage of skin histology generally ends at 25 years old or so, from hereafter growth with aging simultaneously into
Row, elastic fibers of skin is gradually thicker, and 40~50 the beginning of the year old phase, the aging of skin is slowly obvious.The aging of skin be it is a kind of from
So rule, anti-aging is eternal beauty topic, although we cannot prevent the natural aging of skin, can pass through science
Effective measures change the aging state of skin.
Ferment is that one kind contains enzyme and its microbes producing cellulase and the associated adjustment factor and interaction factor, is such as activated
The substance of agent and inhibitor, collaboration and the feedback regulation factor etc..There are a variety of ferment in human skin, to help epidermal cell
Regeneration and the secretion of sebaceous glands, sweat gland.As the age increases, internal ferment is opposite to be reduced, skin aging.Utilize modern biotechnology
Prepared by technology is added in beauty product the ferment of skin beneficiating, to reinforce the function of skin, improves skin.Publication No.
It is by malt, ginseng, core that the Chinese patent application of 105012225 A of CN, which discloses a kind of skin care ferment and its application, the ferment,
Several Chinese medicines of peach kernel etc. ten are added saccharomycetes to make fermentation and are prepared, the ferment can activating skin cells, promote new old
Metabolism, effectively reparation aged skin, and improve oxidation resistance simultaneously, can be used for preparing facial mask, but the system of the skin care ferment
Standby technique is relative complex, cumbersome.
In addition, ferment is applied in skin care item, there are still some problems: ferment molecule is unstable, and life cycle is short, one
As ferment in the intracorporal life cycle of body by a few hours to several weeks etc., well below the shelf life of skin care item；Ferment is usual
Stable not as good as other cosmetic materials, activity is easy to be influenced by soda acid, high temperature and surfactant, therefore in ferment
It needs to pay special attention in cosmetic preparation process；Ferment is in cosmetics such as improper use, it may appear that pruitus, decortication and allergy etc.
Adverse reaction.Therefore, ferment is incorporated into skin care item, is not easy thing, China is to the understanding of ferment cosmetics also in a step
Section, the product for actually entering market is relatively fewer, and most of cosmetics containing ferment are foreign brand name, such as European Biotherm,
Japanese Shiseido, Kosé, the face shop of South Korea etc..
Therefore, it is necessary to which it is mild, stable, non-stimulated to provide a kind of property, it is readily produced, it is quality controllable, and have preferable
The ferment skin care item of skin-care effect are to meet the market demand.
Summary of the invention
The object of the present invention is to provide a kind of anti-ageing activating essence and preparation method thereof containing ferment.The Essence
Containing there are many active skull cap components, capable of effectively penetrating into skin histology, mutually coordinated performance moisturizing water conservation, desalination wrinkle, it is anti-ageing,
The effect of whitening spot-removing, while property is stable, highly-safe, without any adverse reaction.
The present invention is achieved by the following technical programs:
A kind of anti-ageing activating essence containing ferment, including following components and its parts by weight: 0.5~2 part of allantoin, sweet
1~4 part of oil, 2~4 parts of hydrolecithin, 0.5~2 part of caprylic/capric triglyceride, 6~9 parts of plant enzyme, high mountain rose Du
Cuckoo floral leaf stem cell cultivates 1~3 part of extract, 0.05~0.1 part of citronellol, 0.01~0.03 part of EDTA- disodium, preservative
0.1~0.5 part and 65~75 parts of deionized water.
It is preferred that the anti-ageing activating essence containing ferment, including following components and its parts by weight: allantoin 1.5
Part, 3 parts of glycerol, 3 parts of hydrolecithin, 1.5 parts of caprylic/capric triglyceride, 8 parts of plant enzyme, high mountain rose indian azalea leaf
2 parts of extract of stem cell culture, 0.05 part of citronellol, 0.02 part of EDTA- disodium, 0.3 part of preservative and 70 parts of deionized water.
The plant enzyme includes that following parts by weight prepare raw material: 10~20 parts of oat, 10~20 parts of tremella and sweet
5~15 parts of grass.
It is preferred that the plant enzyme includes that following parts by weight prepare raw material: 15 parts of oat, 15 parts of tremella and Radix Glycyrrhizae 10
Part.
The plant enzyme is prepared by the following method to obtain, method includes the following steps:
(1) oat, tremella, the Radix Glycyrrhizae of formula ratio are weighed, is mixed, is crushed, the water of 10~12 times of its dry weight is added, impregnates 1
~2h, decocts 1~2h, and filtering obtains filter residue and Aqueous extracts；
(2) Aqueous extracts are concentrated into the one third of original volume at 55~60 DEG C, obtain concentrate；
(3) concentrate that filter residue that step (1) obtains, step (2) obtain is added into fermentor, it is warming up to 100~
120 DEG C, sterilize 30~45min, is cooled to 25~30 DEG C, and Bacillus acidi lactici is added, then Cattell is added in 48~72h of anaerobic fermentation
Saccharomycete is passed through clean compressed air toward fermentor, carries out under conditions of 25~30 DEG C, 400~800rpm/min aerobic
48~72h of fermentation by filtering fermentation liquor, obtains supernatant after fermentation；
(4) supernatant that step (3) obtains is filtered using sterilizing filter, collects bacteria-free filtrate, concentrates the filtrate to
The one third of original volume, obtains plant enzyme.
The inoculum concentration of Bacillus acidi lactici is the 2~4% of fermentation material total weight in the preparation step (3) of the plant enzyme,
The inoculum concentration of saccharomyces carlsbergensis bacterium is the 1~3% of fermentation material total weight.
The preservative be selected from Phenoxyethanol, methyl hydroxybenzoate, Sensiva SC50, phenyllactic acid, methylisothiazolinone,
At least one of 2,4- chlorobenzyl alcohol or methyl p-hydroxybenzoate.
It is preferred that the preservative is Phenoxyethanol and Sensiva SC50 with 1:(0.5~1) mass ratio form.More
It is preferred that the preservative is to be formed from Phenoxyethanol and Sensiva SC50 with the mass ratio of 1:1.
The present invention also provides a kind of preparation method of above-mentioned anti-ageing activating essence containing ferment, the preparation method include with
Lower step: removing ionized water, allantoin, glycerol, EDTA- disodium, and agitated kettle is added and stirs evenly after being heated to 70~75 DEG C,
It is pumped into vacuum emulsifying machine, is added hydrolecithin, caprylic/capric triglyceride, citronellol, booting stirring, in 800~
1000rpm/min mixes 30~45min, is cooled to 35~40 DEG C；Plant enzyme, the training of high mountain rose indian azalea leaf stem cell is added
Extract and preservative are supported, continues to stir evenly, be cooled to room temperature, it is filling to get Essence.
Anti-ageing activating essence provided by the invention containing ferment contains a variety of active skull cap components, including plant enzyme,
High mountain rose indian azalea leaf stem cell cultivates extract etc., each ingredient interaction, and collaboration plays good moisturizing water conservation, desalination
Wrinkle, anti-ageing, whitening spot-removing effect, while skin is not stimulated, skin care activity ingredient stability is good, is easy to Transdermal absorption.
The plant enzyme is formed by 3 kinds of oat, tremella and Radix Glycyrrhizae raw materials through microbial fermentation, and fermentation process safely may be used
Control, tunning is pollution-free, non-stimulated, highly-safe, contains microelement abundant, amino acid, oligopeptides, vitamin, mineral
Matter, enzyme isoreactivity ingredient can help activating skin cells, promote metabolism, effectively reparation aged skin, improve anti-oxidant energy
Power increases skin water content.High mountain rose indian azalea leaf stem cell cultivates the epigenetic factor that extract includes
Human adult stem cell provides nutrient, effectively makes skin epidermal stem cell, corium stem cell, subcutaneous tissue stem cell activation, energy
Reaching skin corium, the active constituent in plant stem cell can promote the power of regeneration of skin, improve epidermis and corium from inside to outside,
To protect and maintain the function of human adult cell, skin histology is made to be updated reparation and delay senescence, improve skin quality, restores
The ecological environment of Clinical application of skin situ regeneration medical technique duplication.
Ingredient in inventive formulation is unless otherwise instructed commercial product.Wherein, high mountain rose indian azalea leaf is thin
Born of the same parents cultivate the Phytocelltec that extract is the production of the biochemical corp Switzerland meter Bai LeTMAlp Rose, the rose leaf stem cell mention
Taking object is the powder-product based on the wild rose leaf stem cell of Alps, and main component is that high mountain rose azalea is extracted
Object, different malt, lecithin, sodium benzoate, lactic acid and water.
Compared with prior art, the invention has the following advantages:
(1) provided by the invention to go deep into skin bottom quickly by skin overall absorption containing the anti-ageing activating essence of ferment
Water lock while supplement is horizontal, hence it is evident that improve and have dry skin coarse situation, effectively calm down skin lines, promote skin new old generation
It thanks, the improvement colour of skin is obscure, improves the colour of skin, and the sensitive skin that can releive, and makes the soft and smooth delicacy of skin, and high resilience reappears skin beauty
Beautiful life, long-time service can effective delay skin aging, achieve the purpose that the tender skin of bright face.
(2) the anti-ageing activating essence each component provided by the invention containing ferment mutually cooperates with, multiple reparation skin, and safety
It is non-stimulated, stable system, while preparation method is simple, condition is controllable, process stabilizing, can be applicable.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.
The preparation of 1 plant enzyme of embodiment
Plant enzyme includes that parts by weight shown in following table prepare raw material:
| Raw material | A group | B group | C group | D group |
| Oat | 15 | 20 | 10 | 15 |
| Tremella | 15 | 10 | 20 | 20 |
| Radix Glycyrrhizae | 10 | 15 | 10 | 5 |
Preparation step:
(1) oat, tremella, the Radix Glycyrrhizae of formula ratio are weighed, is mixed, is crushed, the water of 12 times of its dry weight is added, impregnates 1.5h,
2h is decocted, filtering obtains filter residue and Aqueous extracts；
(2) Aqueous extracts are concentrated into the one third of original volume at 60 DEG C, obtain concentrate；
(3) concentrate that filter residue that step (1) obtains, step (2) obtain is added into fermentor, is warming up to 100 DEG C,
Sterilize 45min, is cooled to 25 DEG C, and Bacillus acidi lactici is added, and the inoculum concentration of Bacillus acidi lactici is the 3% of fermentation material total weight, anaerobism hair
Then saccharomyces carlsbergensis bacterium is added in ferment 72h, the inoculum concentration of saccharomyces carlsbergensis bacterium is the 2% of fermentation material total weight, is passed through toward fermentor clean
Net compressed air carries out aerobic fermentation 48h, after fermentation, by fermentation liquid mistake under conditions of 30 DEG C, 500rpm/min
Filter, obtains supernatant；
(4) supernatant that step (3) obtains is filtered using sterilizing filter, collects bacteria-free filtrate, concentrates the filtrate to
The one third of original volume, obtains plant enzyme.
The preparation of anti-ageing activating essence of the embodiment 2 containing ferment
Anti-ageing activating essence containing ferment includes that parts by weight shown in following table prepare raw material:
Preparation step: removing ionized water, allantoin, glycerol, EDTA- disodium, and agitated kettle is added and stirs after being heated to 75 DEG C
It mixes uniformly, is pumped into vacuum emulsifying machine, hydrolecithin, caprylic/capric triglyceride, citronellol is added, booting is stirred, in
800rpm/min mixes 45min, is cooled to 35 DEG C；Plant enzyme is added, high mountain rose indian azalea leaf stem cell cultivates extract
And preservative, continue to stir evenly, be cooled to room temperature, it is filling to get Essence.
The preparation of anti-ageing activating essence of the comparative example 1 containing ferment
Anti-ageing activating essence of the comparative example 1 containing ferment and the difference of the formula of embodiment 21 are, are free of plant enzyme, tool
Body includes following components and its parts by weight: 1.5 parts of allantoin, 3 parts of glycerol, 3 parts of hydrolecithin, caprylic/capric glycerol three
1.5 parts of ester, high mountain rose indian azalea leaf stem cell cultivate 2 parts of extract, 0.05 part of citronellol, 0.02 part of EDTA- disodium, anti-corrosion
0.3 part and 70 parts of deionized water of agent.
The preparation of anti-ageing activating essence of the comparative example 2 containing ferment
Anti-ageing activating essence of the comparative example 2 containing ferment and the difference of the formula of embodiment 21 are, shut out without high mountain rose
Cuckoo floral leaf stem cell cultivates extract, specifically includes following components and its parts by weight: 1.5 parts of allantoin, 3 parts of glycerol, hydrogenation
3 parts of lecithin, 8 parts of plant enzyme, 0.05 part of citronellol, 0.02 part of EDTA- disodium, is prevented at 1.5 parts of caprylic/capric triglyceride
0.3 part and 70 parts of deionized water of rotten agent.
The preparation of anti-ageing activating essence of the comparative example 3 containing ferment
Anti-ageing activating essence of the comparative example 3 containing ferment and the difference of the formula of embodiment 21 are, thin with apple stem cell
Born of the same parents cultivate extract replacement high mountain rose indian azalea leaf stem cell and cultivate extract, specifically include following components and its parts by weight
Number: 1.5 parts of allantoin, 3 parts of glycerol, 3 parts of hydrolecithin, 1.5 parts of caprylic/capric triglyceride, 8 parts of plant enzyme, apple
2 parts of stem cells culture extract, 0.05 part of citronellol, 0.02 part of EDTA- disodium, 0.3 part of preservative and deionized water 70
Part.
The apple stem cells culture extract is the production of the biochemical corp Switzerland meter Bai Le
PhytocelltecTMMalus Domestica, the dried apple slices cell extract are based on rare Switzerland apple stem cell
And develop liposomes product, main component be Switzerland's apple berry extract, xanthan gum, glycerol, lecithin, Phenoxyethanol and
Water.
Test example one, Product Safety evaluation (experiment of human skin patch)
(1) trial target: embodiment 2 is formulated Essence made from Essence made from 1-5 and comparative example 1-3.
Tested crowd's number: 80 people, women, the age: between 20~50 years old, subjects skin's health, no skin disease allergy
History meets subject and volunteers inclusion criteria, grouping: 8 groups, every group of 10 people, and average age 30 ± 2 years old.
(2) patch method: selecting qualified spot tester, with closed patch experiment method, by tested material about 0.02~
For 0.025mL drop in spot tester, external application special adhesive tape is covered on subject back, and tested material is removed after 24 hours, is removed respectively
Observation dermoreaction in 0.5,6,12,24,48 hour afterwards, records it according to dermoreaction grade scale in " cosmetics health specification "
As a result.
(3) experimental result: human skin patch test result is shown: each group subject is formulated 1-5, right by embodiment 2
The patch test of Essence made from ratio 1-3, in observation dermoreaction in 0.5,6,12,24,48 hour, wherein 0 skin occurs
It is safe to illustrate that the Essence uses for skin adverse reaction, nonirritant.
Test example two, product be crease-resistant, moisturizing effect test
Essence made from 1-5 and comparative example 1-3 is formulated to embodiment 2 respectively and carries out crease-resistant, moisturizing measure of merit.
(1) test method: choosing 80 healthy volunteers, and face has an obvious wrinkle, the age between 35~55 years old it
Between.All subjects are randomly divided into 8 test groups, every group of 10 people, respectively test 1-5 group and comparison 1-3 group, wherein testing
1-5 group is formulated Essence made from 1-5 using embodiment 2 respectively, and comparison 1-3 group is respectively using essence made from comparative example 1-3
Liquid.Appropriate Essence is taken uniformly to be applied to face after subject's morning and evening face cleaning of all test groups, 2~3min of massage is inhaled to complete
It receives.Trial employment period prevents solar exposure, and is forbidden to use other skin care item.After continuous use 1 month, all subjects are carried out
Anti-wrinkle, the detection of moisturizing effect and record.
(2) effect assessment standard
Skin care item of the present invention are evaluated using the variation of the change rate and moisture content of skin of dermatoglyph degree and elasticity
Anti-wrinkle, moisturizing effect, the specific method is as follows: 1. dermatoglyph degree: utilizing German skin image analysis system (Skin
Visiometer SV600) measurement subject's face skin texture situation.Before on-test, first to each subject
The texture of skin detected, and record；Test 1 month after, again to the texture of the skin of each subject into
Row detection, and record.The texture rate of descent of skin: texture rate of descent=(numerical value-examination before testing is calculated using following formula
Test rear numerical value)/test preceding numerical value × 100%.Texture rate of descent is bigger, shows that anti-wrinkle effect is better；2. skin elasticity: utilizing
Skin elasticity tester (Cutometer dual MPA580) measures the elastic numerical value change situation of subject's face skin to comment
The elastic stage of valence skin, numerical value is higher, and surface elasticity of skin is better.Before on-test, first to each subject's skin
Elasticity is detected, and is recorded；After test 1 month, the elasticity of each subject's skin is detected again, and records.
The climbing of the elasticity of skin is calculated using following formula: before elastic climbing=(numerical value after numerical value-test before testing)/test
Numerical value × 100%.Elastic climbing is bigger, shows that anti-wrinkle effect is better.3. skin moisture content: utilizing the skin of CK company, Germany
The moisture content of skin water tester (Corneometer CM825) measurement subject's face skin.It is first right before on-test
The moisture content of each subject's skin detects, and records；After test 1 month, again to each subject's skin
Moisture content detected, and record.The climbing of skin moisture content: moisture content climbing is calculated using following formula
Numerical value × 100% before=(numerical value before numerical value-test after test)/test.Moisture content climbing is bigger, shows that moisturizing effect is got over
It is good.
It the results are shown in Table 1.
The Essence of the present invention of table 1 is crease-resistant, moisturizing measure of merit result
The results show that after the trial volunteer of test 1-5 group uses embodiment 2 to be formulated Essence made from 1-5 respectively, skin
The average texture degree rate of descent of skin is up to 46.2%, and average elasticity climbing is up to 38.4%, and average water content climbing is up to
70.5%, hence it is evident that better than the service condition of comparison 1-3 group trial volunteer.The above result shows that Essence provided by the invention
It is formulated the mutually coordinated gain of each ingredient, plays optimal crease-resistant, moisturizing effect jointly.
Test example three, the test of product whitening effect
Essence made from 1-5 and comparative example 1-3 is formulated to embodiment 2 respectively and carries out whitening effect test.
(1) test method: 80 healthy volunteers are chosen, the age is between 25~55 years old.By all subjects
It is randomly divided into 8 test groups, every group of 10 people, respectively test 1-5 group and comparison 1-3 group, wherein test 1-5 group is respectively using real
It applies example 2 and is formulated Essence made from 1-5, comparison 1-3 group is respectively using Essence made from comparative example 1-3.All test groups
Appropriate Essence is taken uniformly to be applied to face after subject's morning and evening face cleaning, 2~3min of massage is to fully absorbing.Trial employment period prevents
Solar exposure, and it is forbidden to use other skin care item.After continuous use 1 month, all subjects are carried out with the detection of whitening effect
And record.
(2) effect assessment standard
The whitening effect of skin care item of the present invention is evaluated using the change rate of dermal melanin content and brightness, specific side
Method is as follows: 1. dermal melanin content: using the black of Japanese Minolta CD-2600 spectrophotometric analysis subject's face skin
Pigment content situation of change.It uses Lab color system, collects the reflected light vertical with skin surface, in measurement parameter
B reflection is from Huang to blue color variation range (value variation range :+60 yellow~-60 blues), with the black pigment of skin content in just
Correlation, this is related with melanin reflection yellow light.Before on-test, first the melanin content of each subject's skin is carried out
Detection, and record；After test 1 month, the melanin of each subject's skin is detected again, and records.Utilize with
Lower formula calculates the melanin rate of descent of skin: melanin rate of descent=(numerical value after numerical value-test before testing)/numerical value before testing
× 100%.Melanin content rate of descent is bigger, shows that whitening effect is better.2. skin brightness: utilizing skin measurement system
(VISIA) brightness of skin is measured, the skin the bright, is worth higher, and the skin the dim, is worth lower.Before on-test,
First the brightness of each subject's skin is detected, and is recorded；After test 1 month, again to each subject's flesh
The brightness of skin is detected, and is recorded.Utilize following formula calculate skin brightness change rate: brightness change rate=
(numerical value before numerical value-test after test)/test preceding numerical value × 100%.Brightness climbing is bigger, shows that whitening effect is better.
It the results are shown in Table 2.
The Essence whitening effect test result of the present invention of table 2
| Group | Average black element rate of descent (%) | Average luminance climbing (%) |
| Test 1 group | 39.2 | 20.6 |
| Test 2 groups | 36.4 | 18.4 |
| Test 3 groups | 32.8 | 16.0 |
| Test 4 groups | 37.5 | 19.2 |
| Test 5 groups | 35.6 | 17.6 |
| Compare 1 group | 24.8 | 11.4 |
| Compare 2 groups | 23.2 | 10.8 |
| Compare 3 groups | 28.8 | 14.6 |
The results show that after the trial volunteer of test 1-5 group uses embodiment 2 to be formulated Essence made from 1-5 respectively, skin
The average black element rate of descent of skin is up to 39.2%, and average luminance climbing is up to 20.6%, hence it is evident that better than comparison 1-3 group will
It is willing to the service condition of subject.The above result shows that the mutually coordinated gain of each ingredient of essence formula of liquid provided by the invention, jointly
Play optimal whitening effect.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (9)
1. a kind of anti-ageing activating essence containing ferment, which is characterized in that including following components and its parts by weight: allantoin
0.5~2 part, 1~4 part of glycerol, 2~4 parts of hydrolecithin, 0.5~2 part of caprylic/capric triglyceride, plant enzyme 6~9
Part, high mountain rose indian azalea leaf stem cell culture 1~3 part of extract, 0.05~0.1 part of citronellol, EDTA- disodium 0.01~
0.03 part, 0.1~0.5 part of preservative and 65~75 parts of deionized water.
2. the anti-ageing activating essence according to claim 1 containing ferment, which is characterized in that including following components and its again
Measure number: 1.5 parts of allantoin, 3 parts of glycerol, 3 parts of hydrolecithin, 1.5 parts of caprylic/capric triglyceride, 8 parts of plant enzyme,
High mountain rose indian azalea leaf stem cell cultivates 2 parts of extract, 0.05 part of citronellol, 0.02 part of EDTA- disodium, 0.3 part of preservative
With 70 parts of deionized water.
3. the anti-ageing activating essence according to claim 1 or 2 containing ferment, which is characterized in that the plant enzyme
Raw material is prepared including following parts by weight: 10~20 parts of oat, 10~20 parts of tremella and 5~15 parts of Radix Glycyrrhizae.
4. the anti-ageing activating essence according to claim 3 containing ferment, which is characterized in that the plant enzyme includes
Following parts by weight prepare raw material: 15 parts of oat, 15 parts of tremella and 10 parts of Radix Glycyrrhizae.
5. the anti-ageing activating essence according to claim 3 containing ferment, which is characterized in that the system of the plant enzyme
It is standby the following steps are included:
(1) oat, tremella, the Radix Glycyrrhizae of formula ratio are weighed, is mixed, is crushed, the water of 10~12 times of its dry weight is added, impregnates 1~2h,
1~2h is decocted, filtering obtains filter residue and Aqueous extracts；
(2) Aqueous extracts are concentrated into the one third of original volume at 55~60 DEG C, obtain concentrate；
(3) concentrate that filter residue that step (1) obtains, step (2) obtain is added into fermentor, is warming up to 100~120
DEG C, sterilize 30~45min, is cooled to 25~30 DEG C, and Bacillus acidi lactici is added, then saccharomyces carlsbergensis is added in 48~72h of anaerobic fermentation
Bacterium is passed through clean compressed air toward fermentor, aerobic fermentation is carried out under conditions of 25~30 DEG C, 400~800rpm/min
48~72h by filtering fermentation liquor, obtains supernatant after fermentation；
(4) supernatant that step (3) obtains is filtered using sterilizing filter, collects bacteria-free filtrate, concentrates the filtrate to substance
Long-pending one third, obtains plant enzyme.
6. the anti-ageing activating essence according to claim 5 containing ferment, which is characterized in that the system of the plant enzyme
The inoculum concentration of Bacillus acidi lactici is the 2~4% of fermentation material total weight in standby step (3), and the inoculum concentration of saccharomyces carlsbergensis bacterium is fermentation material
The 1~3% of total weight.
7. according to the anti-ageing activating essence of any of claims 1 or 2 containing ferment, which is characterized in that the preservative is selected from
Phenoxyethanol, methyl hydroxybenzoate, Sensiva SC50, phenyllactic acid, methylisothiazolinone, 2,4- chlorobenzyl alcohol or P-hydroxybenzoic acid
At least one of methyl esters.
8. according to the anti-ageing activating essence as claimed in claim 7 containing ferment, which is characterized in that the preservative is benzene oxygen second
Pure and mild Sensiva SC50 is with 1:(0.5~1) mass ratio form.
9. a kind of method for preparing the anti-ageing activating essence described in claim 1 containing ferment, which is characterized in that including following
Step: removing ionized water, allantoin, glycerol, EDTA- disodium, and agitated kettle is added and stirs evenly after being heated to 70~75 DEG C, takes out
Enter in vacuum emulsifying machine, is added hydrolecithin, caprylic/capric triglyceride, citronellol, booting stirring, in 800~
1000rpm/min mixes 30~45min, is cooled to 35~40 DEG C；Plant enzyme, the training of high mountain rose indian azalea leaf stem cell is added
Extract and preservative are supported, continues to stir evenly, be cooled to room temperature, it is filling to get Essence.
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| CN110960481A (en) * | 2019-12-20 | 2020-04-07 | 广州市拉凯尔干细胞研究所 | Preparation method of skin care composition for increasing exosome yield |
| CN111904908A (en) * | 2020-08-19 | 2020-11-10 | 上海怡宝化妆品集团有限公司 | Essence containing rose fermentation liquor and preparation method thereof |
| CN113304099A (en) * | 2021-07-15 | 2021-08-27 | 杭州宏果科技有限公司 | Rose energy cream and preparation method thereof |
| CN115109739A (en) * | 2022-08-03 | 2022-09-27 | 肽源（广州）生物科技有限公司 | Method for rapidly culturing desert rose suspension cells at high density to produce chlorogenic acid at high yield |
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Application publication date: 20190301 |