CN109368107B

CN109368107B - Biological sample storage library and storage method thereof

Info

Publication number: CN109368107B
Application number: CN201811330237.0A
Authority: CN
Inventors: 瞿建国; 罗沉; 刘玲玲; 王建信
Original assignee: Shanghai OriginCell Biological Cryo Equipment Co Ltd
Current assignee: Shanghai OriginCell Biological Cryo Equipment Co Ltd
Priority date: 2018-11-09
Filing date: 2018-11-09
Publication date: 2024-10-11
Anticipated expiration: 2038-11-09
Also published as: CN109368107A; JP3235995U; WO2020093585A1

Abstract

The invention discloses a biological sample storage warehouse, which comprises a storage warehouse box body, a plurality of deep low-temperature storage tanks and an automatic transfer mechanism, wherein the storage warehouse box body is provided with a plurality of storage tanks; the at least one cryogenic storage tank and the automatic transfer mechanism are all arranged in the storage warehouse box body; the automatic transfer mechanism can receive a transfer tank which is transmitted from the outside through the transfer window and stores the frozen storage tube; the automatic transfer mechanism can transfer the received frozen storage pipe in the transfer tank into the deep low-temperature storage tank; the problem that the biological sample storage warehouse in the prior art cannot ensure full cold chain protection is solved, the damage risk is increased for the storage of biological samples, and the problem that the deep low-temperature storage tank in the prior art is very inconvenient to transport is solved; the invention also provides a storage method of the biological sample repository.

Description

Biological sample storage library and storage method thereof

Technical Field

The invention relates to the field of biological sample storage equipment, in particular to a biological sample storage library and a storage method thereof.

Background

A biological sample library is a device that standardizes the collection, processing, storage of samples of biological macromolecules, cells, tissues, and organs of healthy and diseased organisms. Before storing, the biological sample needs to be dormant by program cooling and then is stored in a liquid nitrogen container or a cryogenic refrigerator. For certain specific biological samples, the storage process needs to ensure the protection of an all-cold chain, and the whole process needs to be in a deep low-temperature environment to protect the biological activity and avoid repeated freeze thawing damage.

The existing storage warehouse is characterized in that a plurality of deep low-temperature storage tanks are arranged in a fixed warehouse, when a freezing storage pipe is required to be stored or adjusted, the freezing storage pipe is manually put into the warehouse, and the deep low-temperature storage tanks stored in corresponding categories are found for manual storage; when the biological sample is stored, the biological sample manually enters a warehouse, a deep low-temperature storage tank is opened, and a freezing pipe is placed into the deep low-temperature storage tank from a transferring tank; it should be further noted here that the biological sample is placed in the cryopreservation vessel at the beginning of extraction, and the cryopreservation vessel is placed in the transfer pot for convenience of transfer and transportation. The prior art has more limitations on the storage of the freezing storage tube through manual operation: 1. during the manual work, the freezing pipe or the transfer tank is always in a non-low temperature environment in the storage process, so that a large destructive risk is brought to the biological sample; 2, the biological sample is manually stored and enters a warehouse, a classified deep low-temperature storage tank of the biological sample is required to be found firstly, and then the biological sample is stored, so that the efficiency is low, the labor cost is high, the biological sample is not easy to store in a classified manner, and the risk is high; 3. when the deep low-temperature storage tank is opened manually to store the freezing pipe, the opening size of the deep low-temperature storage tank must conform to the operation degree of a person, the opening of the deep low-temperature storage tank in the process has destructive effect on the temperature of the deep low-temperature storage tank, and the deep low-temperature storage tank is taken as a liquid nitrogen tank as an example, so that the nitrogen consumption in operation can be great; 4. the manual storage is easy to generate the influence and mutual pollution between the mutual movements of the samples, and the risk is high; 5. the existing warehouse type arrangement mode is inconvenient to install and transport, and has poor field adaptability; 6. the subsequent maintenance of the deep cryogenic storage warehouse is very inconvenient.

The way can not ensure that the freezing tube can ensure the protection of the whole cold chain in the whole process of storing or transferring; not only does this create a significant risk of damage to the biological sample within the cryopreservation vessel, but for certain specific biological samples, this prior art storage mode appears to be undesirable when the requirements for full cold chain protection are high;

when the biological sample is required to be classified and transferred integrally, the biological sample is also very inconvenient; the deep low-temperature storage tank is large in volume and is inconvenient to set and transport;

In a word, the prior art has poor storage mode, the access tube is relatively inconvenient, and inconvenience is brought to users.

Disclosure of Invention

An object of the present invention is to provide a biological sample storage library, which solves the problem that the biological sample storage library in the prior art cannot ensure full cold chain protection, and the problem that the risk of damage is increased for the storage of biological samples, and solves the problem that the transportation of a deep low-temperature storage tank in the prior art is very inconvenient.

Another object of the present invention is to provide a storage method for a biological sample repository, which solves the problems of inconvenient storage mode, unable to ensure cold chain protection in the whole course, low storage efficiency and inconvenient transportation in the prior art.

The invention solves the first technical problem by adopting the following technical scheme: a biological sample storage warehouse comprises a storage warehouse box body, a plurality of cryogenic storage tanks and an automatic transfer mechanism; the at least one cryogenic storage tank and the automatic transfer mechanism are all arranged in the storage warehouse box body; the automatic transfer mechanism can receive a transfer tank which is transmitted from the outside through the transfer window and stores the frozen storage tube; and the automatic transfer mechanism can transfer the received frozen storage pipe in the transfer tank into the deep low-temperature storage tank.

Preferably, a basket at a movable position is arranged in the cryogenic storage tank, a plurality of tray supporting pieces are arranged in the basket, and a freezing tube tray is supported on the tray supporting pieces; the deep cryogenic storage tank is provided with a storage door, and each tray support can move along with the movement of the lifting basket so as to move to a position corresponding to the storage door; the automatic transfer mechanism comprises a shoveling component, and the shoveling component can be used for shoveling and receiving the frozen tube tray in the basket through the storage door or can be used for shoveling and returning the received frozen tube tray into the basket.

Preferably, the shoveling assembly comprises a shoveling box body, a shoveling first driving piece, a shoveling second driving piece, a shoveling sliding base, a sliding guide plate, a shoveling disc assembly and a temporary storage frame; a shoveling conveying opening is formed in the shoveling box body, and the shoveling conveying opening can correspond to a storage door of the cryogenic storage tank; the temporary storage frame and the shoveling sliding base are arranged in the shoveling box body, the sliding guide plate is slidably arranged on the shoveling sliding base, and the shovel disc assembly is slidably arranged on the sliding guide plate; the temporary storage frame can temporarily store the transfer tank; the shoveling box body is provided with a shoveling nitrogen hole, and the shoveling nitrogen hole is communicated with a nitrogen supply system; the shovel first driving piece drives the sliding of the sliding guide plate, and the shovel second driving piece drives the sliding of the shovel disc assembly.

Preferably, the shoveling sliding base comprises a left sliding base, a right sliding base and a base frame, wherein the left sliding base and the right sliding base are respectively arranged at the left side and the right side of the base frame;

the sliding guide plate comprises a left sliding guide rail, a right sliding guide rail and a sliding plate; the left sliding guide rail and the right sliding guide rail are respectively arranged at the left side end and the right side end of the sliding plate;

The shovel disc assembly comprises a shovel disc left sliding part, a shovel disc right sliding part and a shovel disc; the left sliding part of the shovel disc and the right sliding part of the shovel disc are arranged on the left side and the right side of the shovel disc;

The outside of left slip guide rail is in the slip of left sliding seat is in the slip of right slip guide rail is in the slip of right sliding seat is in, shovel dish left side slider is in the slip of left slip guide rail is in the inboard, shovel dish right side slider is in the slip of right slip guide rail is inboard.

Preferably, the left sliding seat comprises a sliding supporting plate and a plurality of pulleys, and the pulleys are arranged on the sliding supporting plate in a row; the right sliding seat and the left sliding seat are identical in structure and are symmetrically arranged;

the left sliding guide rail is I-shaped, and the right sliding guide rail and the left sliding guide rail are identical in structure and symmetrically arranged;

The shovel first driving piece comprises a shovel driving motor and a screw rod, a screw rod nut is arranged on the lower end face of the sliding plate, the screw rod nut is in threaded connection with the screw rod, and a motor shaft of the shovel driving motor is connected with the screw rod.

Preferably, the automatic transferring mechanism further comprises a code scanning component, and the code scanning component can scan the code of the frozen storage tube transferred by the automatic transferring mechanism.

Preferably, the automatic transfer mechanism further comprises a transfer receiving box body and a manipulator;

The transferring and receiving box body and the shoveling and sending box body are arranged side by side, and the manipulator is arranged above the shoveling and sending assembly and the transferring and receiving box body;

the transfer receiving box body receives the transfer tank conveyed in from the transfer window; the manipulator can grab the transfer tank;

The shovel box body is characterized in that a shovel receiving port is formed in the upper end face of the shovel box body, and the manipulator can move to a position corresponding to the shovel receiving port.

Preferably, the automatic transfer mechanism further comprises a transfer housing; the manipulator comprises a grabbing driving assembly, a Z shaft sleeve, a Z shaft lever, a Y shaft guide rail, a first X shaft guide rail, a second X shaft guide rail, a grabbing mechanism and a pipe fitting;

The first X-axis guide rail and the second X-axis guide rail are fixedly distributed at two side ends of the transferring shell, and the two side ends of the Y-axis guide rail are slidably arranged on the first X-axis guide rail and the second X-axis guide rail; the Z shaft sleeve is vertically arranged, and the middle part of the Z shaft sleeve is slidably arranged on the Y shaft guide rail;

the Z shaft rod is slidably arranged in the Z shaft sleeve, a Z bearing connector is arranged at the lower end of the Z shaft rod, and the tank grabbing mechanism and the pipe taking piece are respectively arranged on the Z bearing connector;

The tank grabbing mechanism can grab the transfer tank, and the pipe taking piece can take the frozen storage pipe;

the grabbing driving assembly is used for driving the movement of the Z shaft sleeve, the Y shaft guide rail and the Z shaft rod respectively.

Preferably, a rotating frame, a rotary driving assembly and a lifting member are arranged in the cryogenic storage tank;

The lifting member is nested in the rotating frame, and the upper part of the lifting member is fixed with the tank body of the cryogenic storage tank; the rotary driving assembly drives the rotary frame, so that the rotary frame rotates around the lifting member in the cryogenic storage tank;

The rotating frame is divided into a plurality of storage areas by a plurality of dividing plates, lifting operation channels are formed in the storage areas, and each lifting operation channel is communicated with the lifting member;

a basket is arranged in the storage area; the periphery of the basket extends outwards to form a pushing receiving block, and the pushing receiving block penetrates through the lifting operation channel to extend to a position corresponding to the lifting member;

the lifting member can drive the lifting basket to do lifting motion along the lifting operation channel in the storage area through the action of the pushing receiving block.

Preferably, a transition axial groove is concavely formed in the middle part above the rotating frame, a plurality of partition plates are outwards arranged on the periphery of the transition axial groove in an extending mode, and a plurality of lifting operation channels are formed in the circumferential groove wall of the transition axial groove; the lifting operation channels are in one-to-one correspondence with the storage areas divided by the partition plates;

The lifting member comprises a lifting sleeve, a lifting driving motor, a lifting driving wheel, a lifting driven wheel, a lifting transition wheel and a lifting table;

the lifting sleeve is arranged in the transition axial groove and can rotate relative to the rotating frame; the upper circumference of the lifting sleeve is outwards extended to form a lifting transition plate, the lifting drive motor is arranged outside the deep low-temperature storage tank, and a motor shaft of the lifting drive motor downwards extends to sequentially penetrate through a tank cover of the deep low-temperature storage tank and the lifting transition plate to form a lifting driving wheel;

The upper end of the periphery of the lifting sleeve is provided with a lifting transition wheel which is meshed with the lifting driving wheel, the lower end of the periphery of the lifting sleeve is provided with a lifting driven wheel, and a lifting conveying belt is arranged between the lifting driven wheel and the lifting transition wheel; the lifting transmission belt is provided with a lifting table, and the lifting table corresponds to the pushing receiving block.

Preferably, a storage guide sliding rail is arranged on the partition plate, a basket sliding strip matched with the storage guide sliding rail in a direction is also arranged on the periphery of the basket, and the basket sliding strip slides on the storage guide sliding rail.

Preferably, the lifting sleeve is a hollow pipe sleeve;

The center of the bottom of the transition axial groove extends to the inside of the lifting sleeve, and the upper end of the rotating action shaft is provided with a cross-shaped receiving groove;

the rotary driving assembly comprises a rotary driving motor and a rotary driving shaft, the rotary driving motor is fixed outside the deep low-temperature storage tank, a motor shaft of the rotary driving motor is coaxially connected with the rotary driving shaft, a cross-shaped clamping piece is arranged at the lower end of the rotary driving shaft, and the clamping piece of the rotary driving shaft is clamped in the receiving groove.

Preferably, the basket is fan-shaped, and a plurality of support bars are arranged on two sides in the basket, and each two support bars on the same horizontal plane form the tray support piece.

Preferably, the freezing pipe tray is in a sector shape matched with the lifting basket, and a plurality of pipe storage grooves are formed in the freezing pipe tray, and the bottoms of the pipe storage grooves are development type bottoms.

Preferably, the system further comprises an access door, a nitrogen supply system and a dehumidification system, wherein the access door is arranged on the storage box body, and the storage box body can be opened through the access door to overhaul the inside of the storage box body;

the nitrogen supply system can supply nitrogen into the storage box body and also can supply nitrogen into the automatic transfer mechanism;

The dehumidification system can dehumidify the storage box body.

The invention solves the other technical problem by adopting the following technical scheme: r, directly placing a transfer tank with a freezing pipe into a storage warehouse through a transfer window of a storage warehouse box body; s, automatically transferring the received frozen storage pipes in the transfer tank into the deep low-temperature storage tank by the automatic transfer mechanism in the storage warehouse.

Preferably, S1, a manipulator of an automatic transfer mechanism grabs a transfer tank received by a storage warehouse onto a set temporary storage frame;

S2, simultaneously with the step S1 or after the step R, the automatic transfer mechanism moves to a corresponding deep low-temperature storage tank along a designated line;

S3, a storage door of the deep low-temperature storage tank is opened, and a shoveling and conveying assembly of the automatic transfer mechanism shovels a frozen storage tube tray in the deep low-temperature storage tank onto a platform corresponding to the automatic transfer mechanism;

s4, grabbing a frozen storage pipe in the transfer tank on the temporary storage frame by a manipulator, and placing the frozen storage pipe on a frozen storage pipe tray;

s5, conveying the freezing pipe tray back to the deep low-temperature storage tank by the shoveling and conveying assembly.

Preferably, when the step S4 is performed, the manipulator grabs the frozen storage tube from the temporary storage frame to the upper part of the code scanning component to scan the two-dimensional code on the frozen storage tube, and the set control system processes and stores the code scanning result, and then grabs the frozen storage tube to the frozen storage tube tray.

Preferably, before the step S3, the corresponding basket in the cryogenic storage tank is moved to a position corresponding to the storage door;

The step T is divided into:

T1, driving the rotating frame to rotate by the rotary driving assembly of the deep low-temperature storage tank, so as to drive the lifting basket on the rotating frame to move to a position corresponding to the storage door of the deep low-temperature storage tank in the circumferential direction;

And T2, lifting the basket by the lifting component, so that the freezing storage pipe tray on the basket is lifted to a position where the shoveling component can shovel the freezing storage pipe tray through the storage door.

Preferably, the method further comprises the step G: the storage box body is directly transported by a transport vehicle provided with a container.

The invention has the following first beneficial effects:

1. the biological sample storage warehouse is convenient for integral transportation, convenient to install, strong in field adaptability and strong in expansibility;

2. The storage operation is reliable, the efficiency is high, and the storage capacity is large;

3. The storage is convenient and the classification is easy;

4. energy conservation and effective reduction of energy (liquid nitrogen) consumption;

5. The influence between the mutual movements of the samples and the risk of mutual pollution can be reduced;

6. The maintenance is convenient, and the device can be quickly replaced in case of faults.

The invention has the following second beneficial effects: the storage method can be used for fast storage, guarantees whole-course cold chain storage, fast and accurate classified storage, and is high in efficiency, and labor cost is greatly reduced.

Drawings

FIG. 1 is a schematic diagram of a biological sample repository structure;

FIG. 2 is an exploded schematic view of a biological sample repository structure;

FIG. 3 is a top view of a biological sample reservoir;

FIG. 4 is a schematic diagram of the internal structure of the cryogenic storage tank;

FIG. 5 is a cross-sectional view of a cryogenic storage tank;

FIG. 6 is a schematic view of a rotating frame and a lifting member;

FIG. 7 is a schematic view of the structure of the basket from the rear view;

FIG. 8 is a schematic view of the structure of the basket from the front view;

FIG. 9 is a schematic view of a lifting member;

FIG. 10 is a schematic diagram of a rotary drive assembly and lift drive motor;

FIG. 11 is a schematic view of an automated transfer mechanism;

FIG. 12 is a schematic view of the internal structure of the automatic transfer mechanism;

FIG. 13 is a schematic view of a shovel assembly and a manipulator;

fig. 14 is an exploded view of the shovel assembly.

The labels in the figures are:

1-a repository box; 101-a transfer window;

2-a deep cryogenic storage tank; 201-basket; 202-a tray support; 203-a cryopreservation tube tray; 204-a storage gate; 205-rotating rack; 206-a storage area; 207-lifting members; 208-a rotary drive assembly; 209-a lift operation path; 2010-pushing the receiving block; 2011-transition axial slots; 2012-dividing plates; 2013-lifting the sleeve; 2014-a lift drive motor; 2015-lifting drive wheel; 2016-lifting driven wheel; 2017-lifting transition wheels; 2018-a lifting table; 2019-lifting the transition plate; 2020-lifting conveyor belt; 2022-store guide skid track; 2023-basket slide; 2024-rotating the action shaft; 2025-receiving slot; 2026-a rotary drive motor; 2027-rotating the drive shaft; 2028-clip; 2029-support bar; 2030-a pipe storage tank;

3-an automatic transfer mechanism;

301-a shoveling assembly; 3011-shoveling the box body; 3012-scooping the first driving member; 3014-shoveling the sliding base; 3015-sliding guide plate; 3016-a shovel disk assembly; 3017-a temporary storage rack; 3018-spading a delivery port; 3019-shoveling the nitrogen hole; 30110-left slide base; 30111-right slide base; 30112-a base frame; 30113-left slide guide rail; 30114-right slide guide rail; 30115-a sliding plate; 30116-a left slider of the shovel disk; 30117-a shovel pan right slider; 30118-a shovel disk; 30119-a sliding support plate; 30120-pulley; 30121-a shoveling driving motor; 30122-screw rod; 30123-lead screw nut; 30124-scooping the receiving opening;

302, a code sweeping component; 3021-a scanner; 3022-a code scanning mirror; 3023-a scanner mounting slot;

303-a transfer receiving box; 3031-a first opening; 3032-a second opening; 3033-transport upper cover;

304-a manipulator; 3041-a grip drive assembly; 3042-Z sleeve; 3043-Z shaft; 3044-Y axis guide rail; 3045-first X-axis guide rail; 3046-a second X-axis guide rail; 3047-a can-grabbing mechanism; 3048-taking out a pipe fitting; 3049-Z bearing connector;

305-a transport housing;

4-a transfer tank; 5-access door; 6-a nitrogen supply system; 7-a dehumidifier; 8-automatic transfer track.

Detailed Description

The technical scheme of the invention is further described below with reference to the embodiment and the attached drawings.

Example 1

The embodiment provides a biological sample storage warehouse, which comprises a storage warehouse box body 1, a plurality of cryogenic storage tanks 2 and an automatic transfer mechanism 3; the at least one cryogenic storage tank 1 and the automatic transfer mechanism 3 are arranged in the storage box body 1; the automatic transfer mechanism 3 can receive a transfer tank 4 which is transmitted from the outside through the transfer window 101 and stores the frozen storage tube, and the transfer window 101 is arranged on the storage box body 1; and the automatic transfer mechanism 3 can transfer the received frozen storage pipe in the transfer tank 4 into the cryogenic storage tank 2.

A storage box body 1 is arranged, a plurality of deep low temperature storage tanks 2 are arranged in the storage box body 1, an automatic operation mechanism 3 is arranged among the plurality of deep low temperature storage tanks 2, the automatic operation mechanism 3 can move among the plurality of deep low temperature storage tanks 2, the automatic transfer mechanism 3 can correspond to a transfer window 101 of the storage box body 1, a transfer tank 4 with a frozen storage tube enters the automatic transfer mechanism 3 through the transfer window 101, the automatic transfer mechanism 3 carries the transfer tank 101 to move to the corresponding deep low temperature storage tank 2, and the frozen storage tube of the transfer tank 101 is transferred into the deep low temperature storage tank 2 for storage; of course, a control system is arranged here to control the operation of the cryogenic storage tank 2 and the automatic transfer mechanism 3; the nitrogen supply system is arranged to supply nitrogen to the cryogenic storage tank 2 and keep a cryogenic state, and can also continuously supply nitrogen to the automatic operation mechanism 3 so as to keep the cryogenic state.

The biological sample storage warehouse 1 is characterized in that a plurality of deep low-temperature storage tanks are arranged in the storage warehouse box body, so that the on-site installation is convenient, the adaptability is strong, and the expansibility is strong; the number, the position and the like of the deep low-temperature storage tanks in the storage box body can be set according to the field requirement; when the storage box is transported, the storage box is convenient to transport, and the storage box is directly transported; 2. the destructive risk brought in the manual storage process in the prior art is changed, so that the transfer tank or the freezing tube is always in a low-temperature environment, and the destructive risk in the storage process is greatly reduced; 3, the warehouse does not need to be entered manually, the storage warehouse box body directly receives the transfer tank, the transfer tank is transferred by the automatic transfer mechanism, the transfer tank is in butt joint with the corresponding deep low-temperature storage tank, the corresponding classified storage can be fast carried out, the accuracy and the high efficiency are realized, and the risk is greatly reduced; 4. when the automatic transfer mechanism is in butt joint with the deep low-temperature storage tank for storage, only a port with a corresponding size of the middle transfer tank which can be stored can be opened, and the storage is rapid and in place, so that the risk that the low-temperature storage environment in the deep low-temperature storage tank is damaged when the deep low-temperature storage tank is opened for storage by manpower is avoided, the nitrogen consumption is greatly reduced, and the stability is high; 5, reducing the influence among sample interaction movements and the risk of mutual pollution; 6. the subsequent maintenance is very convenient.

The embodiment further discloses a structural mode of butt joint conveying and storing the freezing storage pipe between the automatic transfer mechanism 3 and the cryogenic storage tank 2; a basket 201 at a movable position is arranged in the cryogenic storage tank 2, a plurality of tray supports 202 are arranged in the basket 201, and a freezing pipe tray 203 is supported on the tray supports 202; the cryogenic storage tank 2 is provided with a storage door 204, and each of the tray supports 202 is movable with the basket 201 so as to be moved to a position corresponding to the storage door 204; the automated transfer mechanism 3 includes a scooping assembly 301, the scooping assembly 301 may scoop up the vial pallet 203 within the basket 201 through the storage door 204 or may scoop up the vial pallet 203 back into the basket 201. In order to ensure the stability of the automatic transfer mechanism 3 to transfer the frozen storage pipes, and also to reduce the size of a storage door 204 which is opened when the cryogenic storage tank 2 is in butt joint with the automatic transfer mechanism 3 as small as possible, the cryogenic environment inside the cryogenic storage tank 2 is not influenced, so that a shoveling component 301 matched with the cryogenic storage tank 2 is arranged, a movable basket 201 is arranged in the cryogenic storage tank 2, a plurality of tray supporting pieces 202 are arranged on the basket 201, the tray supporting pieces 202 are propped up with the frozen storage pipe trays 203, the shoveling component 301 penetrates through the storage door 204 and enters the frozen storage pipe trays 203 corresponding to the basket 201 to be shoveled out, then the automatic transfer mechanism 3 shovels the frozen storage pipes in the received transfer tank 4 onto the frozen storage pipe trays 203, and the frozen storage pipe trays 203 with the frozen storage pipes are shoveled back into the cryogenic storage tank 2; not only ensures the stability of the transportation of the freezing storage tube, but also reduces the environmental impact on the low-temperature storage tank 2 in the transportation process as much as possible. Of course, the docking mode of the automatic transfer mechanism 3 and the cryogenic storage tank 2 may be a mode of sucking or grabbing by a manipulator, or a mode of directly moving the basket out of the cryogenic storage tank 2 and docking the automatic transfer mechanism 3, and the like, which cannot provide a stable and effective transfer mode, and even if the transfer achieves the transfer purpose, the mode without the preferred shoveling component in the embodiment is more stable, has higher efficiency, and has no influence on the environment. As shown in fig. 3, 4 cryogenic storage tanks 2 are arranged in the storage tank body 1, an automatic transfer rail 8 is arranged between the 4 cryogenic storage tanks 2, and the automatic transfer mechanism 3 moves on the automatic transfer rail 8, so that the purpose of moving between the 4 cryogenic storage tanks 2 is achieved.

The embodiment further discloses a specific implementation manner of the shoveling assembly 301, where the shoveling assembly 301 includes a shoveling box 3011, a shoveling first driving member 3012, a shoveling second driving member, a shoveling sliding base 3014, a sliding guide plate 3015, a shoveling disc assembly 3016 and a temporary storage frame 3017; a shoveling and conveying opening 3018 is formed in the shoveling and conveying box 3011, and the shoveling and conveying opening 3018 can correspond to a storage door 204 of the cryogenic storage tank 2; the temporary storage frame 3017 and the shovel sliding base 3014 are both arranged in the shovel box body 3011, the sliding guide plate 3015 is slidably arranged on the shovel sliding base 3014, and the shovel disk assembly 3016 is slidably arranged on the sliding guide plate 3015; the temporary storage frame 3017 can temporarily store the transfer tank 4; a shoveling nitrogen hole 3019 is formed in the shoveling box body 3011, and the shoveling nitrogen hole 3019 is communicated with a nitrogen supply system; the shovel first driving member 3012 drives the sliding of the sliding guide plate 3015, and the shovel second driving member drives the sliding of the shovel disk assembly 3016.

The automatic transfer mechanism 3 temporarily stores the transfer tank 4 received from the transmission window 101 of the storage box body 1in the temporary storage frame 3017, a shoveling box body 3011 is arranged, a shoveling nitrogen through hole 3019 is formed in the shoveling box body 3011, the shoveling nitrogen through hole 3019 is communicated with a nitrogen supply system, so that the continuous nitrogen supply in the shoveling box body 3011 is ensured to be in a deep low temperature state all the time, and the received transfer tank 4 is also in the deep low temperature state immediately; when the transfer tank 4 is temporarily stored in the temporary storage frame 3017, the automatic transfer mechanism 3 has moved to a position corresponding to the cryogenic storage tank 2, and the shovel delivery port 3018 corresponds to the storage door 204 of the cryogenic storage tank 2; the first shoveling driving piece 3012 drives the sliding guide plate 3015 to move towards the shoveling conveying opening 3018, then the second shoveling driving piece 3013 drives the shovel disk assembly 3016 to move out of the shoveling conveying opening 3018 and extend into the cryogenic storage tank 2 to shoveling the corresponding frozen tube tray 203, and then the frozen tube tray is retracted to the initial position; after the freezing pipe tray 203 is brought into the automatic transfer mechanism, the freezing pipe in the transfer tank 4 is placed into the freezing pipe tray 204; the frozen tube tray 203 after receiving the frozen tube is shoveled back into the deep cryogenic storage tank 2 by the shoveling and conveying component 301 for storage; of course, the door opening mode of the storage door 204 of the cryogenic storage tank 2 is set to be a mode of sliding up and down to be opened, so that the door opening mode is more convenient to match with the butt joint of the automatic transfer mechanism 3.

The arrangement 1 is that the whole process is in a deep low-temperature environment, so that the safety of biological samples is greatly improved; 2. the temporary storage rack provides temporary storage positions for the transfer tank, so that the frozen storage pipes in the transfer tank can be conveniently extracted; 3. the shoveling assembly is high in stability in the shoveling process.

While ensuring stable operation of the shoveling process, in order to reduce the structural occupation space of the shoveling assembly 301 as much as possible, so that the structure is compact, and the nitrogen supply energy source is saved, this embodiment further preferably discloses a further implementation mode of the shoveling assembly 301, the shoveling sliding base 3014 includes a left sliding base 30110, a right sliding base 30111 and a base frame 30112, and the left sliding base 30110 and the right sliding base 30111 are respectively arranged at the left side and the right side of the base frame 30112; the sliding guide plate 3015 includes a left sliding guide rail 30113, a right sliding guide rail 30114, and a sliding plate 30115; the left sliding guide rail 30113 and the right sliding guide rail 30114 are respectively arranged at the left and right side ends of the sliding plate 30115; the shovel disk assembly 3016 comprises a shovel disk left sliding part 30116, a shovel disk right sliding part 30117 and a shovel disk 30118; the shovel disk left sliding part 30116 and the shovel disk right sliding part 30117 are arranged at the left side and the right side of the shovel disk 30118; the outer side of the left slide guide track 30113 slides on the left slide base 30110, the outer side of the right slide guide track 30114 slides on the right slide base 30111, the blade left slide 30116 slides on the inner side of the left slide guide track 30113, and the blade right slide 30117 slides on the inner side of the right slide guide track 30114.

The left sliding seat 30110 includes a sliding support plate 30119 and a plurality of pulleys 30120, the plurality of pulleys 30120 being disposed in a row on the sliding support plate 30119; the right sliding seat 30111 and the left sliding seat 30110 have the same structure and are symmetrically arranged; the left sliding guide rail 30113 is in an i shape, and the right sliding guide rail 30114 and the left sliding guide rail 30113 have the same structure and are symmetrically arranged; the shoveling first driving member 3012 comprises a shoveling driving motor 30121 and a screw rod 30122, a screw rod nut 30123 is arranged on the lower end face of the sliding plate 30115, the screw rod nut 30123 is in threaded connection with the screw rod 30122, and a motor shaft of the shoveling driving motor 30121 is connected with the screw rod 30122.

A shovel driving motor of the shovel first driving piece 3012 drives a screw 30122 to rotate, the screw 30122 is in threaded connection with a screw nut 30123, and the rotary motion is converted into linear motion to drive a sliding plate 30115 to linearly move; the left sliding guide rail 30113 is in an i-shaped structure, so that one side end of the left sliding guide rail 30113 is matched with a plurality of pulleys 30120 of the left sliding seat 30110 to slide on a sliding support plate 30119; meanwhile, the other side end of the I-shaped form a sliding track of the shovel disk left sliding part 30116, so that the shovel disk left sliding part 30116 slides on the sliding track; the right sliding guide rail 30114 and the left sliding guide rail 30113 have the same structure and are symmetrically arranged; this arrangement is compact in space scale and stable in operation while providing sufficient telescopic length of the shovel assembly 301.

In a preferred further implementation manner of this embodiment, the automatic transferring mechanism 3 further includes a code scanning member 302, and the code scanning member 302 may scan the code of the frozen storage tube transferred by the automatic transferring mechanism. After receiving the transfer tank 4, the automatic transfer mechanism 3 temporarily stores the transfer tank 4 through a temporary storage frame 3017, extracts a frozen storage pipe from the transfer tank 4, moves the frozen storage pipe to a code scanning component 302, scans codes of the frozen storage pipe, and then sends the frozen storage pipe into the deep low-temperature storage tank 2 through a shovel; the code scanning is used for processing, analyzing and storing the information of the freezing storage tube, so that high-standard automation is realized. Further disclosed herein is a code scanning component 302 comprising a scanner 3021 and a code scanning mirror 3022, wherein a code scanner mounting groove 3023 can be arranged in the automatic transfer mechanism 3, the code scanner 3021 is mounted in the code scanner mounting groove 3023, and the code scanning mirror 3022 is covered above the code scanner mounting groove 3023; thus facilitating code scanning.

In order to better receive the transfer tank 4 and transfer the transfer tank 4 and cooperate with the shovel assembly 301, the automatic transfer mechanism 3 further includes a transfer receiving box 303 and a manipulator 304; the transferring and receiving box 303 is arranged side by side with the shoveling and sending box 3011, and the manipulator 304 is arranged above the shoveling and sending assembly 301 and the transferring and receiving box 303; the transfer receiving box 303 receives the transfer tank 4 fed in from the transfer window 101; the manipulator 304 can grab the transfer pot 4; a shovel receiving opening 30124 is formed in the upper end face of the shovel box 3011, and the manipulator 304 is movable to a position corresponding to the shovel receiving opening 30124. A first opening 3031 and a second opening 3032 are formed on the transfer receiving box 303, the first opening 3031 is correspondingly attached to the transfer window 101, the second opening 3032 is formed on the upper end surface of the transfer receiving box 303, and a transfer upper cover 3033 can be arranged at the second opening 3032; the first opening 3031 receives the transfer pot 4, the transfer window 101 is closed, the transfer upper cover 3033 at the second opening 3032 is opened, the manipulator 304 penetrates through the second opening 3032 to grasp the transfer pot 4 and put the transfer pot 4 into the temporary storage frame 3017, the shoveling and conveying assembly 301 shovels the frozen tube tray 203 into the shoveling and conveying box 3011 of the automatic transfer mechanism 3, and the manipulator 304 grasps the frozen tube in the transfer pot 4 on the temporary storage frame 3017; firstly grabbing to a code scanning component, scanning codes, and processing and storing the information of the freezing storage tube; the manipulator 304 grabs and moves the frozen tube to the shovel receiving opening 30124, and places the frozen tube into the frozen tube tray 203 corresponding to the position below the shovel receiving opening 30124; the shovel assembly 301 then shovel the frozen tube tray 203 back into the cryogenic storage tank 2 for storage.

In a preferred further implementation of this embodiment, the automatic transfer mechanism 3 further comprises a transfer housing 305; the manipulator 304 includes a grabbing driving assembly 3041, a Z-axis sleeve 3042, a Z-axis shaft 3043, a Y-axis guide rail 3044, a first X-axis guide rail 3045, a second X-axis guide rail 3046, a grabbing mechanism 3047, and a grabbing pipe 3048; the first X-axis guide rail 3045 and the second X-axis guide rail 3046 are distributed and fixed at two side ends of the transferring housing 305, and two side ends of the Y-axis guide rail 3044 are slidably disposed on the first X-axis guide rail 3045 and the second X-axis guide rail 3046; the Z-axis sleeve 3042 is vertically disposed, and the middle part of the Z-axis sleeve 3042 is slidably disposed on the Y-axis guide rail 3044; the Z shaft 3043 is slidably disposed in the Z shaft sleeve 3042, a Z bearing connector 3049 is disposed at the lower end of the Z shaft 3043, and the tank grabbing mechanism 3047 and the pipe fitting 3048 are disposed on the Z bearing connector 3049 respectively; the tank grabbing mechanism 3047 can grab the transfer tank 4, and the pipe grabbing piece 3048 can grab the frozen storage pipe; the gripping driving assembly 3041 drives the movement of the Z-axis sleeve 3042, the Y-axis guide rail 3044, and the Z-axis shaft 3043, respectively.

The two side ends of the Y-axis guide rail 3044 can slide along the first X-axis guide rail 3045 and the second X-axis guide rail 3046 to realize movement in the X-axis direction; the Z shaft sleeve can slide on the Y-axis guide rail 3044 to realize movement in the Y-axis direction; meanwhile, the Z shaft lever 3043 can vertically move up and down in the Z shaft sleeve 3042, so that the movement in the Z shaft direction is realized; as shown in fig. 13, a Z-axis receiver 3049 is disposed at the lower end of the Z-axis receiver 3043, and a tank grabbing mechanism 3047 and a pipe fitting 3048 are disposed at the left and right ends of the Z-axis receiver 3049, wherein the tank grabbing mechanism 3047 can grab the transfer tank 4, and the pipe fitting 3048 can take out the freezing storage pipe; firstly, the transfer tank 4 is grabbed onto the temporary storage frame 3017 by utilizing the tank grabbing mechanism 3047, and the tank cover of the transfer tank 4 is opened, wherein the tank cover can be grabbed and opened by the tank grabbing mechanism 3047, and other cover opening modes can be adopted, so that the description is omitted; after the cover is opened, the pipe fitting 3048 in the transfer pot 4 is grabbed by the pipe fitting, then the code scanning is carried out, and the subsequent actions such as grabbing the frozen storage pipe tray 203 are carried out.

In order to optimize the storage environment and the matching butt joint of the cryogenic storage tank 2 and the automatic transfer mechanism 3 as much as possible; in the further embodiment of the cryogenic tank 2 of the present embodiment, the basket 201 in the storage area 206 to be stored is rotated to the corresponding position by the rotation of the rotating frame 205, and then the basket 201 is extracted to the position corresponding to the storage door 204 by the lifting member 207; the design of the cryogenic storage tank 2 of the invention aims at fully utilizing the storage space in a limited space, and the whole basket 201 is not lifted, and only the operation of the required freezing storage tube tray 203 is correspondingly carried out with the storage door 204, so that a further optimized implementation mode is provided: a rotating frame 205, a rotary driving assembly 208 and a lifting member 207 are arranged in the cryogenic storage tank 2; The lifting member 207 is nested in the rotating frame 205, and the upper part of the lifting member 207 is fixed with the tank body of the cryogenic storage tank 2; the rotary driving assembly 208 drives the rotary rack 205, so that the rotary rack 205 rotates around the lifting member 207 in the cryogenic storage tank 2; the rotating frame 205 is divided into a plurality of storage areas 206 by a plurality of dividing plates, the storage areas 206 are provided with lifting operation channels 209, and each lifting operation channel 209 is communicated with the lifting member 207; a basket 201 is arranged in the storage area 206; A push receiving block 2010 is arranged at the periphery of the basket 201 in an outward extending manner, and the push receiving block 2010 penetrates through the lifting operation channel 209 to a position corresponding to the lifting member 207; the lifting member 207 may drive the basket 201 to move up and down along the lifting operation path 209 in the storage region 206 by the action of the push receiving block 2010. The lifting member 207 is nested in the rotating frame 205, so that space is saved greatly, and a large amount of space can be saved to be set as a storage area 206; further, a transition axial groove 2011 is concavely formed in the middle of the upper portion of the rotating frame 205, a plurality of partition boards 2012 are outwardly extended from the periphery of the transition axial groove 2011, and a plurality of lifting operation channels 209 are formed on the circumferential groove wall of the transition axial groove 2011; The lifting operation channels 209 are in one-to-one correspondence with the storage areas 206 divided by the partition 2012; the lifting member 207 includes a lifting sleeve 2013, a lifting drive motor 2014, a lifting driving wheel 2015, a lifting driven wheel 2016, a lifting transition wheel 2017, and a lifting platform 2018; the lifting sleeve 2013 is disposed in the transition axial slot 2011 and is rotatable relative to the rotating frame 205; a lifting transition plate 2019 extends outwards from the upper circumference of the lifting sleeve 2013, and the lifting member 207 is fixed with the cryogenic storage tank 2 by the lifting transition plate 2019; The preferred embodiment of the cryogenic storage tank 2 of the present invention is a liquid nitrogen tank; the lifting driving motor 2014 is disposed outside the cryogenic storage tank 2, and a motor shaft of the lifting driving motor 2014 extends downward to sequentially pass through a tank cover of the cryogenic storage tank 2 and the lifting transition plate 2019 to be provided with a lifting driving wheel 2015; the upper end of the periphery of the lifting sleeve 2013 is provided with a lifting transition wheel 2017 which is in meshed connection with the lifting driving wheel 2015, wherein the lifting driving wheel 2015 is preferably a sector gear which is in arc-shaped meshed with the sector lifting transition wheel 2017 and is arranged in the form of most saving moment; a lifting driven wheel 2016 is arranged at the lower end of the periphery of the lifting sleeve 2013, and a lifting conveying belt 2020 is arranged between the lifting driven wheel 2016 and the lifting transition wheel 2017; A lifting table 2018 is provided on the lifting belt 2020, and the lifting table 2018 corresponds to the push receiving block 2010.

After the rotation driving assembly 208 drives the rotation frame 205 to rotate, i.e., each storage area 206 rotates in the axial direction, the basket 201 rotates along with the storage areas 206; because the lifting operation channel 209 is formed in the transition axial slot 2011, that is, the rotating frame 205, a pushing receiving block 2010 is formed on the periphery of the basket 201 of each storage area 206 in an extending manner, so that not only is the stability of the basket 201 in the circumferential direction along with the rotation of the storage area 206 ensured, but also when the corresponding basket 201 is required, the basket 201 is stopped at the position corresponding to the lifting member 207, and of course, the lifting member 207 always maintains the position corresponding to the storage door 204 in the circumferential direction; the lifting driving motor 2014 drives the lifting driving wheel 2015 to drive the lifting transition wheel 2017, so that the lifting conveyor 2020 is driven to move on the lifting transition wheel 2017 and the lifting driven wheel 2016; since the actual position of the lifting platform 2018 is kept lower than the position of the push receiving block 2010 where the basket 201 extends toward the lifting operation channel 209, when the lifting conveyor 2020 moves, the lifting platform 2018 on the lifting conveyor 2020 pushes the push receiving block 2010 to move upward when moving to the position of the storage door 204, thereby driving the basket 201 to move upward along the lifting operation channel 209 until the required movement of the freezing tube tray 203 corresponds to the position of the storage door 204.

In a preferred further implementation manner of this embodiment, a storage guide runner 2022 is provided on the partition 2012, and a basket slide 2023 that is matched with the storage guide runner 2022 in a direction of the periphery of the basket 201 is also provided, and the basket slide 2023 slides on the storage guide runner 2022. Enhancing stability of the lifting movement of the basket 201.

In a preferred further implementation of this embodiment, the lifting sleeve 2013 is a hollow-interior pipe sleeve; a rotation acting shaft 2024 extends towards the inside of the lifting sleeve 2013 at the center of the bottom of the transition axial groove 2011, and a cross-shaped receiving groove 2025 is arranged at the upper end of the rotation acting shaft 2024; the rotary driving assembly 208 comprises a rotary driving motor 2026 and a rotary driving shaft 2027, the rotary driving motor 2026 is fixed outside the cryogenic storage tank 2, a motor shaft of the rotary driving motor 2026 is coaxially connected with the rotary driving shaft 2027, a cross-shaped clamping piece 2028 is arranged at the lower end of the rotary driving shaft 2026, and the clamping piece 2028 of the rotary driving shaft 2027 is clamped in the receiving groove 2025.

In a preferred further implementation manner of this embodiment, the basket 201 is fan-shaped, and a plurality of support bars 2029 are disposed on two sides in the basket 201, and each two support bars 2029 on the same horizontal plane form the tray support 202.

In a preferred further embodiment of the present embodiment, the freezing tube tray 203 is in a sector shape that fits with the basket 201, and a plurality of tube storage grooves 2030 are provided on the freezing tube tray 203, and the groove bottom of the tube storage groove 2030 is a development groove bottom. The open tank bottom is provided with an open hole as shown in fig. 6, and the freezing pipe can be inserted into the open tank bottom for more stable storage.

The preferred further implementation manner of the embodiment further comprises an access door 5, a nitrogen supply system and a dehumidification system, wherein the access door 5 is arranged on the storage box body 1, and the storage box body 1 can be opened through the access door 5 to access the interior of the storage box body; the nitrogen supply system can supply nitrogen into the storage box body 1 and also can supply nitrogen into the automatic transfer mechanism; the dehumidification system can dehumidify the storage box 1, and comprises a plurality of dehumidifiers 7. The maintenance is convenient, and the device can be quickly replaced in case of faults.

Example 2

The embodiment provides a storage method of a biological sample storage library, which comprises the following steps of R, directly putting a transfer tank with a freezing storage tube into the storage library through a transmission window of a storage library box body; s, automatically transferring the received frozen storage pipes in the transfer tank into the deep low-temperature storage tank by the automatic transfer mechanism in the storage warehouse. The storage mode abandons the risk of damaging the environment of the freezing storage pipe caused by manual storage in the prior art, and is convenient, efficient and accurate.

In a preferred further implementation manner of the embodiment, step S1, a manipulator of an automatic transfer mechanism grabs a transfer tank received by a storage warehouse onto a set temporary storage frame;

The automatic transfer mechanism is utilized to move among the plurality of deep low-temperature storage tanks, so that the process of manually searching for butt joint among the plurality of deep low-temperature storage tanks in the prior art is replaced, the deep low-temperature storage tanks are more convenient to classify and set, and the storage efficiency is greatly improved. The automatic transfer mechanism is in butt joint with the deep low-temperature storage tank in a shoveling mode, so that the efficiency of storing the frozen storage tube into the deep low-temperature storage tank is greatly improved.

In a preferred further implementation manner of this embodiment, when the step S4 is performed, the manipulator first grabs the frozen storage tube from the temporary storage frame to the position above the code scanning component to scan the two-dimensional code on the frozen storage tube, and the set control system processes and stores the code scanning result, and then grabs the frozen storage tube onto the frozen storage tube tray.

In a preferred further implementation manner of this embodiment, before the step S3 is performed, the corresponding basket in the cryogenic storage tank is moved to a position corresponding to the storage door;

The step T is divided into:

A preferred further implementation manner of this embodiment further includes step G: the storage box body is directly transported by a transport vehicle provided with a container. One container can be transported, and the installation is convenient, and the site adaptability and the expansibility are strong.

The sequence of the above embodiments is only for convenience of description, and does not represent the advantages and disadvantages of the embodiments.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. The biological sample storage warehouse is characterized by comprising a storage warehouse box body, a plurality of deep low-temperature storage tanks and an automatic transfer mechanism; at least one cryogenic storage tank and an automatic transfer mechanism are both arranged in the storage box body, a basket at a movable position is arranged in the cryogenic storage tank, a plurality of tray supporting pieces are arranged in the basket, and a freezing storage tube tray is supported on the tray supporting pieces; the deep cryogenic storage tank is provided with a storage door, and each tray support can move along with the movement of the lifting basket so as to move to a position corresponding to the storage door;

The automatic transfer mechanism comprises a shoveling component, the shoveling component can shovel and receive a frozen tube tray in the basket through a storage door or can shovels and returns the received frozen tube tray into the basket, and the shoveling component comprises a shoveling box body, a shoveling first driving piece, a shoveling second driving piece, a shoveling sliding base, a sliding guide plate, a shoveling disc component and a temporary storage frame;

a shoveling conveying opening is formed in the shoveling box body, and the shoveling conveying opening can correspond to a storage door of the cryogenic storage tank;

The temporary storage frame and the shoveling sliding base are arranged in the shoveling box body, the sliding guide plate is slidably arranged on the shoveling sliding base, and the shovel disc assembly is slidably arranged on the sliding guide plate;

The temporary storage frame can temporarily store the transfer tank;

The shoveling box body is provided with a shoveling nitrogen hole, and the shoveling nitrogen hole is communicated with a nitrogen supply system;

The shovel conveying device comprises a shovel conveying box body, a shovel conveying first driving piece, a shovel conveying second driving piece, a shovel conveying sliding base, a sliding guide plate, a shovel tray assembly and a temporary storage frame, wherein the shovel conveying first driving piece drives the sliding of the sliding guide plate, the shovel conveying second driving piece drives the sliding of the shovel tray assembly, the shovel conveying assembly comprises a shovel conveying box body, a shovel conveying first driving piece, a shovel conveying second driving piece, a shovel conveying sliding base, a sliding guide plate, a shovel tray assembly and a temporary storage frame, the shovel conveying sliding base comprises a left sliding seat, a right sliding seat and a base frame, and the left sliding seat and the right sliding seat are respectively arranged on the left side and the right side of the base frame;

The outer side of the left sliding guide rail slides on the left sliding seat, the outer side of the right sliding guide rail slides on the right sliding seat, the left sliding part of the shovel disk slides on the inner side of the left sliding guide rail, the right sliding part of the shovel disk slides on the inner side of the right sliding guide rail, the left sliding seat comprises a sliding support plate and a plurality of pulleys, and the pulleys are arranged on the sliding support plate in a row; the right sliding seat and the left sliding seat are identical in structure and are symmetrically arranged;

The first shoveling driving piece comprises a shoveling driving motor and a screw rod, a screw rod nut is arranged on the lower end face of the sliding plate, the screw rod nut is in threaded connection with the screw rod, and a motor shaft of the shoveling driving motor is connected with the screw rod;

The automatic transfer mechanism can receive a transfer tank which is transmitted from the outside through the transfer window and stores the frozen storage tube; the automatic transfer mechanism can transfer the received frozen storage pipe in the transfer tank into the deep low-temperature storage tank, and further comprises a transfer shell;

The automatic transfer mechanism further comprises a code scanning component, the code scanning component can scan the frozen storage tubes transferred by the code scanning component, the automatic transfer mechanism further comprises a transfer receiving box body and a manipulator, the transfer receiving box body and the shoveling box body are arranged side by side, and the manipulator is arranged above the shoveling assembly and the transfer receiving box body; the transfer receiving box body receives the transfer tank conveyed in from the transfer window; the manipulator can grab the transfer tank; the shovel receiving port is formed in the upper end face of the shovel box body, the manipulator can move to a position corresponding to the shovel receiving port, the storage box body is directly used for receiving the transfer tank, the transfer tank is transferred by the automatic transfer mechanism, and the transfer tank is used for butt joint of the corresponding deep low-temperature storage tank; the automatic transfer mechanism only opens a mouth with a corresponding size when being in butt joint storage with the cryogenic storage tank, the manipulator comprises a grabbing driving component, a Z shaft sleeve, a Z shaft lever, a Y shaft guide rail, a first X shaft guide rail, a second X shaft guide rail, a tank grabbing mechanism and a pipe fitting taking out, and a rotating frame, a rotating driving component and a lifting component are arranged in the cryogenic storage tank;

A basket is arranged in the storage area; the periphery of the lifting basket is outwards extended to be provided with a pushing receiving block, the pushing receiving block penetrates through the lifting operation channel to extend to a position corresponding to the lifting component, the lifting basket is fan-shaped, two sides in the lifting basket are provided with a plurality of supporting bars, each two supporting bars on the same horizontal plane form the tray supporting piece, the freezing pipe tray is fan-shaped and matched with the lifting basket, the freezing pipe tray is provided with a plurality of pipe storage grooves, the bottom of each pipe storage groove is a development type groove bottom, and the freezing pipe tray after receiving freezing pipes is shoveled back into the deep low-temperature storage tank through the shoveling and conveying component for storage; the door opening mode of the storage door of the deep low-temperature storage tank is set to be a mode of sliding up and down to be opened;

The lifting member can drive the lifting basket to do lifting motion in the storage area along the lifting operation channel through the action of the pushing receiving block;

The grabbing driving assembly drives the movement of the Z shaft sleeve, the Y shaft guide rail and the Z shaft rod respectively, a transition axial groove is concavely formed in the middle of the upper part of the rotating frame, a plurality of partition plates are outwards arranged on the periphery of the transition axial groove in an extending mode, and a plurality of lifting operation channels are formed in the circumferential groove wall of the transition axial groove; the lifting operation channels are in one-to-one correspondence with storage areas divided by the partition plates, storage guide sliding rails are arranged on the partition plates, basket sliding strips matched with the storage guide sliding rails are also arranged on the peripheries of the baskets, and the basket sliding strips slide on the storage guide sliding rails;

The upper end of the periphery of the lifting sleeve is provided with a lifting transition wheel which is meshed with the lifting driving wheel, the lower end of the periphery of the lifting sleeve is provided with a lifting driven wheel, and a lifting conveying belt is arranged between the lifting driven wheel and the lifting transition wheel; the lifting conveyor belt is provided with a lifting table, the lifting table corresponds to the pushing receiving block and further comprises an access door, a nitrogen supply system and a dehumidification system, the access door is arranged on the storage box body, the storage box body can be opened through the access door to overhaul the inside of the storage box body, and the lifting sleeve is a hollow pipe sleeve in the inside; the center of the bottom of the transition axial groove extends to the inside of the lifting sleeve, and the upper end of the rotating action shaft is provided with a cross-shaped receiving groove; the rotary driving assembly comprises a rotary driving motor and a rotary driving shaft, the rotary driving motor is fixed outside the deep low-temperature storage tank, a motor shaft of the rotary driving motor is coaxially connected with the rotary driving shaft, a cross-shaped clamping piece is arranged at the lower end of the rotary driving shaft, and the clamping piece of the rotary driving shaft is clamped in the receiving groove;

The dehumidification system can dehumidify the storage box body.

2. A storage method using the biological sample repository of claim 1, comprising:

r, directly putting the transfer tank with the freezing storage pipe into a storage warehouse through a transfer window of a storage warehouse box body;

s, automatically transferring the received frozen storage pipes in the transfer tank into the deep low-temperature storage tank by the automatic transfer mechanism in the storage warehouse.

3. The method of storing a biological sample repository according to claim 2, wherein step S is divided into:

s1, grabbing a transfer tank received by a storage warehouse onto a set temporary storage frame by a mechanical arm of an automatic transfer mechanism;

4. The method for storing a biological sample repository according to claim 3, wherein,

When the step S4 is carried out, the manipulator grabs the frozen storage tube from the temporary storage frame to the upper part of the code scanning component to scan the two-dimensional code on the frozen storage tube, the set control system processes and stores the code scanning result, and then grabs the frozen storage tube to the frozen storage tube tray.

5. A method of storing a biological sample repository according to claim 3, comprising: t, before the step S3 is carried out, the corresponding basket in the cryogenic storage tank is moved to a position corresponding to the storage door;

The step T is divided into:

6. The method of claim 5, further comprising the step of G: the storage box body is directly transported by a transport vehicle provided with a container.