CN109276576A - The purposes of plain boiled pork ganoderma lucidum polysaccharide in the preparation of antitumor drugs - Google Patents
The purposes of plain boiled pork ganoderma lucidum polysaccharide in the preparation of antitumor drugs Download PDFInfo
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- CN109276576A CN109276576A CN201811387888.3A CN201811387888A CN109276576A CN 109276576 A CN109276576 A CN 109276576A CN 201811387888 A CN201811387888 A CN 201811387888A CN 109276576 A CN109276576 A CN 109276576A
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- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 62
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 62
- 235000015277 pork Nutrition 0.000 title claims abstract description 58
- 150000004676 glycans Chemical class 0.000 title claims abstract description 50
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 42
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000002246 antineoplastic agent Substances 0.000 title claims abstract description 13
- 229940041181 antineoplastic drug Drugs 0.000 title claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000001556 precipitation Methods 0.000 claims abstract description 12
- 239000012141 concentrate Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 9
- 230000000118 anti-neoplastic effect Effects 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 7
- 239000008194 pharmaceutical composition Substances 0.000 claims description 7
- 238000010992 reflux Methods 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 6
- 230000001476 alcoholic effect Effects 0.000 claims description 5
- 235000009508 confectionery Nutrition 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 238000005342 ion exchange Methods 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 241000640636 Ganoderma leucocontextum Species 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 239000000470 constituent Substances 0.000 claims description 2
- 230000001376 precipitating effect Effects 0.000 claims 1
- 230000004614 tumor growth Effects 0.000 abstract description 9
- 229930012538 Paclitaxel Natural products 0.000 abstract description 7
- 229960001592 paclitaxel Drugs 0.000 abstract description 7
- RCINICONZNJXQF-MZXODVADSA-N taxol Chemical compound O([C@@H]1[C@@]2(C[C@@H](C(C)=C(C2(C)C)[C@H](C([C@]2(C)[C@@H](O)C[C@H]3OC[C@]3([C@H]21)OC(C)=O)=O)OC(=O)C)OC(=O)[C@H](O)[C@@H](NC(=O)C=1C=CC=CC=1)C=1C=CC=CC=1)O)C(=O)C1=CC=CC=C1 RCINICONZNJXQF-MZXODVADSA-N 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 206010028980 Neoplasm Diseases 0.000 description 13
- 241000699666 Mus <mouse, genus> Species 0.000 description 11
- 150000004804 polysaccharides Polymers 0.000 description 8
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 230000000259 anti-tumor effect Effects 0.000 description 5
- 125000002456 taxol group Chemical group 0.000 description 5
- 206010006187 Breast cancer Diseases 0.000 description 4
- 208000026310 Breast neoplasm Diseases 0.000 description 4
- 230000003698 anagen phase Effects 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 241000222336 Ganoderma Species 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000011725 BALB/c mouse Methods 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102100028999 High mobility group protein HMGI-C Human genes 0.000 description 2
- 101000986379 Homo sapiens High mobility group protein HMGI-C Proteins 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000007928 intraperitoneal injection Substances 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000005859 cell recognition Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 125000000468 ketone group Chemical group 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000007472 neurodevelopment Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Mycology (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the purposes of plain boiled pork ganoderma lucidum polysaccharide in the preparation of antitumor drugs, the preparation method of the plain boiled pork ganoderma lucidum polysaccharide is the following steps are included: (1) is extracted；(2) alcohol precipitation；(3) it is lyophilized；(4) it isolates and purifies.It the beneficial effects of the present invention are: providing a kind of new application of plain boiled pork ganoderma lucidum polysaccharide, can effectively inhibit tumour growth, can be used for preparing anti-tumor drug, can be used as the synergist of anti-tumor drug such as taxol etc., there is good medical value.
Description
Technical field
The present invention relates to biomedicine technical fields, and in particular to the use of plain boiled pork ganoderma lucidum polysaccharide in the preparation of antitumor drugs
On the way.
Background technique
Cancer is the world's second largest cause of the death, causes within 2015 8800000 people dead, is had become global serious public strong
Kang Wenti.The important channel that independent or adjunct antineoplastic compound is still anti-tumor drug discovery is screened from natural products.
Polysaccharide is the high molecular polymer connected with ketone group by glycosidic bond by aldehyde radical, serves not only as energy source and support in vivo
Tissue, but prior participation biological phenomena and physiology course such as cell recognition, neurodevelopment, metastases etc., and
Many natural polysaccharide, which have, adjusts the functional activities such as immune, antitumor, hypoglycemic.Ganoderma lucidum is as a kind of food medicine dual-purpose
Macro fungi has long medicinal history in East Asia various countries.Ganoderma lucidum polysaccharide by modern pharmacology prove have protect liver, it is antitumor,
Lowering blood pressure and blood fat adjusts a variety of effects such as immunity of organisms.
Plain boiled pork ganoderma lucidum (Ganoderma leucocontextum) is to acquire in 2011 in Linzhi Bowo County Zha Xi Gang Cun
To morphological feature be different from the ganoderma lucidum of known Ganoderma kind, it is raw according to morphology and molecule by research in 4 years
Object is as a result, be accredited as Ganoderma novel species (Li T et al.2014).The polysaccharide of plain boiled pork ganoderma lucidum fruitbody is detected,
The content of the main component polysaccharide of individual bacterial strains is 6-8 times higher than sold ganoderma lucidum on the market as the result is shown.
The current research in relation to plain boiled pork ganoderma lucidum is only limitted to the extracting method and use of the cultivation of plain boiled pork ganoderma lucidum, identification and extract
Way etc., but seldom it is related to research of the plain boiled pork ganoderma lucidum in terms of inhibition tumour growth and as anti-tumor drug synergist, because
This, which carries out in-depth study in this aspect to it, has significant meaning.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of new application of plain boiled pork ganoderma lucidum polysaccharide, that is, it is used as active constituent
Purposes in the preparation of antitumor drugs has the function of inhibiting tumour growth, while can be combined with antitumor drug paclitaxel,
Enhancing inhibits the effect of tumour growth.
The preparation method of above-mentioned plain boiled pork ganoderma lucidum polysaccharide specifically comprises the following steps:
(1) it extracts: plain boiled pork ganoderma lucidum fruitbody being smashed, is soaked in water, and is heated, refluxing extraction is multiple, merges filter
Filtrate is concentrated liquid, obtains concentrate；
(2) alcohol precipitation: concentrate is subjected to alcohol precipitation, stands overnight, is centrifuged later, precipitated；
(3) it is lyophilized: being dissolved and precipitated with pure water, freeze-drying obtains Thick many candies, and kept dry is spare；
(4) it isolates and purifies: the plain boiled pork ganoderma lucidum crude polysaccharide of acquisition is dissolved in water, stepwise elution is carried out by ion exchange column,
Plain boiled pork ganoderma lucidum polysaccharide is obtained after being freeze-dried again.
Preferably, the preparation method of above-mentioned plain boiled pork ganoderma lucidum polysaccharide may further include following steps:
(1) it extracts: plain boiled pork ganoderma lucidum fruitbody being smashed, is soaked in water half an hour according to the ratio of solid-liquid ratio 1:10~20,
And heated at 80~100 DEG C, three times, merging filtrate concentrates the filtrate to 10th to ten/7th of stoste to refluxing extraction
/ mono-, obtain concentrate；
(2) alcohol precipitation: being sink to final concentration of the 40~80% of alcoholic solvent for concentrate progress alcohol, stand overnight, be centrifuged later,
It is precipitated；
(3) it is lyophilized: being dissolved and precipitated with pure water, freeze-drying obtains Thick many candies, and kept dry is spare；
(4) it isolates and purifies: the plain boiled pork ganoderma lucidum crude polysaccharide of acquisition is dissolved in water, stepwise elution is carried out by ion exchange column,
Plain boiled pork ganoderma lucidum polysaccharide is obtained after being freeze-dried again.
Preferably, the time of refluxing extraction is respectively 2h, 2h, 1h three times in above-mentioned steps (1).
Preferably, the concentrate in above-mentioned steps (2) after alcohol precipitation is stood overnight at a temperature of 4 DEG C, and/or with 4000~
The revolving speed of 8000rpm is centrifuged 10~30min.
It is further preferred that the concentrate in above-mentioned steps (2) after alcohol precipitation is centrifuged 10min with the revolving speed of 6000rpm.
Preferably, above-mentioned steps (2) alcohol is sink to final concentration of the 75% of alcoholic solvent.
Preferably, the plain boiled pork ganoderma lucidum is plain boiled pork ganoderma lucidum Ganoderma leucocontextum HMGIM-
Z110122, deposit number are CCTCC NO:M2016087.
The above-mentioned plain boiled pork ganoderma lucidum of the present invention is picked up from the broad-leaf forest of Linzhi Area of Tibet Bowo County Zha Xi Gang Xiang, is identified as
Plain boiled pork Ganoderma Varieties By Uv Induced, and separation is organized to obtain original strain, it is named as plain boiled pork ganoderma lucidum Ganoderma
Leucocontextum HMGIM-Z110122 has been preserved in China typical culture collection center (letter on March 7th, 2016
Claim CCTCC, address is Wuhan City, Hubei Province Wuchang District Bayi Road Luo Jia Shan), deposit number is CCTCC NO:M2016087.
Further, the present invention also provides a kind of plain boiled pork ganoderma lucidum polysaccharide, and the plain boiled pork ganoderma lucidum polysaccharide is by above-mentioned preparation method
It obtains.
Secondly, the present invention also provides a kind of antineoplastic pharmaceutical compositions, wherein the antineoplastic pharmaceutical compositions contain
Effective quantity plain boiled pork ganoderma lucidum polysaccharide as described above.
Preferably, the antineoplastic pharmaceutical compositions further comprise one or more pharmaceutically acceptable auxiliary materials,
The auxiliary material may include acid, alkali, salt, hydrate or ester and other auxiliary materials.
Compared with prior art, the invention has the following beneficial effects:
The present invention provides a kind of new applications of plain boiled pork ganoderma lucidum polysaccharide, can effectively inhibit tumour growth, can be used for preparing anti-
Tumour medicine, anti-cancer food or health care product can be used as the synergist of anti-tumor drug, have good medical value.
Detailed description of the invention
Fig. 1 is that the tumor weight of each experimental group in experimental example 1 compares figure；
Fig. 2 is that the tumor weight of each experimental group in experimental example 2 compares figure.
Specific embodiment
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
Unless otherwise indicated, reagent and instrument involved in following embodiment can be bought from conventional on the market.
Embodiment 1
The preparation method of plain boiled pork ganoderma lucidum polysaccharide, comprising the following steps:
1) it extracts: plain boiled pork ganoderma lucidum fruitbody being smashed, is soaked in water half an hour according to the ratio of solid-liquid ratio 1:10~20, and
It is heated at 80~100 DEG C, three times, each reflux extracting time is respectively 2h, 2h, 1h to refluxing extraction, and merging filtrate will
Filtrate is concentrated into 1/10th of stoste, obtains concentrate；
2) alcohol precipitation: being sink to final concentration of the 75% of alcoholic solvent for concentrate progress alcohol, stand overnight at a temperature of 4 DEG C, it
10min is centrifuged with the revolving speed of 6000rpm afterwards, is precipitated；
3) it is lyophilized: being dissolved and precipitated with pure water, freeze-drying obtains Thick many candies, and kept dry is spare；
4) it isolates and purifies: the plain boiled pork ganoderma lucidum crude polysaccharide of acquisition is dissolved in water, stepwise elution is carried out by ion exchange column, then
Plain boiled pork ganoderma lucidum polysaccharide is obtained after secondary freeze-drying.
Experimental example 1
1. reagent and instrument
Cell strain: breast cancer mouse cell strain (4T1)；
Mouse: BALB/c mouse.
2. cell culture
Mouse breast cancer is carried out with the DMEM culture solution containing 1% penicillin, 1% streptomysin and 10% fetal calf serum (FBS)
4T1 culture is placed in the middle culture of Thermo Forma3110 incubator for tissue culture (originating from German Thermo company) of 37 DEG C, 5%CO2
To logarithmic growth phase.
3. tumor model zoopery
3.1 is subcutaneous to BABL/C mouse by logarithmic growth phase tumor cell inoculation, and is grouped into model group at random, taxol
Group, plain boiled pork ganoderma lucidum polysaccharide group, taxol+plain boiled pork ganoderma lucidum polysaccharide group；
3.2 second days, start that each group is administered respectively, administration mode is intraperitoneal injection, dosage 50mg/kg/day, mould
Type group be injecting normal saline, successive administration 4 weeks；
Second day after 3.3 last time administrations, mouse was sacrificed in a manner of carbon dioxide, takes tumour, measures tumour weight
Amount and size；
It is antitumor to inhibition mice tumors grew and auxiliary synergy taxol that the plain boiled pork ganoderma lucidum polysaccharide is analyzed in 3.4 calculating
It influences, determines optimal dose.
4. as the result is shown
Table 1
| Group | Tumor weight (g) | Inhibiting rate (%) |
| Model group | 0.255±0.13 | 0 |
| Taxol group | 0.178±0.08 | 30.2±3.0 |
| Polysaccharide group | 0.195±0.1 | 23.5±4.0 |
Note: inhibition rate of tumor growth calculates compared with model group.
Experimental example 2
1. reagent and instrument
Cell strain: breast cancer mouse cell strain (4T1)；
Mouse: BALB/c mouse.
2. cell culture
Mouse breast cancer is carried out with the DMEM culture solution containing 1% penicillin, 1% streptomysin and 10% fetal calf serum (FBS)
4T1 culture, is placed in 37 DEG C, 5%CO2Thermo Forma3110 incubator for tissue culture (originating from German Thermo company) in culture
To logarithmic growth phase.
3. tumor model zoopery
3.1 is subcutaneous to BABL/C mouse by logarithmic growth phase tumor cell inoculation, and is grouped into model group at random, taxol
Group, plain boiled pork ganoderma lucidum polysaccharide group, taxol+plain boiled pork ganoderma lucidum polysaccharide group；
3.2 second days, start that each group is administered respectively, administration mode is intraperitoneal injection, dosage 100mg/kg/day, mould
Type group be injecting normal saline, successive administration 4 weeks；
Second day after 3.3 last time administrations, mouse was sacrificed in a manner of carbon dioxide, takes tumour, measures tumour weight
Amount and size；
It is antitumor to inhibition mice tumors grew and auxiliary synergy taxol that the plain boiled pork ganoderma lucidum polysaccharide is analyzed in 3.4 calculating
It influences, determines optimal dose.
4. as the result is shown
Table 2
| Group | Tumor weight (g) | Inhibiting rate (%) | Increasing Efficiency (%) |
| Model group | 0.143±0.06 | 0 | |
| Taxol group | 0.103±0.07 | 27.8±7 | |
| Polysaccharide group | 0.067±0.05 | 52.9±15 | |
| Polysaccharide+taxol group | 0.034±0.01** | 75.6±21 | 66±14* |
Note: inhibition rate of tumor growth calculates compared with model group；
Increasing Efficiency is polysaccharide+taxol group compared with taxol group.
According to Fig. 1,2 and table 1,2, it can be found that: plain boiled pork ganoderma lucidum polysaccharide has the function of inhibition mouse interior tumor growth,
And the effect that antitumor drug paclitaxel inhibits tumour growth can be significantly increased, is the ganoderma lucidum product with potentiality to be exploited
Kind.
The above, preferable specific embodiment only of the invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. purposes of the plain boiled pork ganoderma lucidum polysaccharide as active constituent in the preparation of antitumor drugs.
2. the preparation method of plain boiled pork ganoderma lucidum polysaccharide, which comprises the steps of:
(1) it extracts: plain boiled pork ganoderma lucidum fruitbody being smashed, is soaked in water, and is heated, refluxing extraction is multiple, merging filtrate, will
Filtrate concentration, obtains concentrate；
(2) alcohol precipitation: concentrate is subjected to alcohol precipitation, stands overnight, is centrifuged later, precipitated；
(3) it is lyophilized: being dissolved and precipitated with pure water, freeze-drying obtains Thick many candies, and kept dry is spare；
(4) it isolates and purifies: the plain boiled pork ganoderma lucidum crude polysaccharide of acquisition is dissolved in water, stepwise elution is carried out by ion exchange column, again
Plain boiled pork ganoderma lucidum polysaccharide is obtained after freeze-drying.
3. the preparation method of plain boiled pork ganoderma lucidum polysaccharide according to claim 2, which is characterized in that the preparation method is further
Include the following steps:
(1) it extracts: plain boiled pork ganoderma lucidum fruitbody being smashed, is soaked in water half an hour according to the ratio of solid-liquid ratio 1:10~20, and
Heated at 80~100 DEG C, refluxing extraction three times, merging filtrate, concentrate the filtrate to stoste 10 to ten/7th/
One, obtain concentrate；
(2) alcohol precipitation: concentrate progress alcohol is sink to final concentration of the 40~80% of alcoholic solvent, stands overnight, is centrifuged later, obtain
Precipitating；
(3) it is lyophilized: being dissolved and precipitated with pure water, freeze-drying obtains Thick many candies, and kept dry is spare；
(4) it isolates and purifies: the plain boiled pork ganoderma lucidum crude polysaccharide of acquisition is dissolved in water, stepwise elution is carried out by ion exchange column, again
Plain boiled pork ganoderma lucidum polysaccharide is obtained after freeze-drying.
4. the preparation method of plain boiled pork ganoderma lucidum polysaccharide according to claim 3, which is characterized in that step flows back three times in (1)
The time of extraction is respectively 2h, 2h, 1h.
5. the preparation method of plain boiled pork ganoderma lucidum polysaccharide according to claim 3, which is characterized in that in step (2) after alcohol precipitation
Concentrate is stood overnight at a temperature of 4 DEG C, and/or is centrifuged 10~30min with the revolving speed of 4000~8000rpm；And/or step
(2) alcohol is sink to final concentration of the 75% of alcoholic solvent.
6. the preparation method of plain boiled pork ganoderma lucidum polysaccharide according to claim 5, which is characterized in that in step (2) after alcohol precipitation
Concentrate is centrifuged 10min with the revolving speed of 6000rpm.
7. the preparation method of plain boiled pork ganoderma lucidum polysaccharide according to claim 3, which is characterized in that the plain boiled pork ganoderma lucidum is plain boiled pork
Ganoderma lucidum Ganoderma leucocontextum HMGIM-Z110122, deposit number are CCTCC NO:M2016087.
8. a kind of plain boiled pork ganoderma lucidum polysaccharide, wherein plain boiled pork ganoderma lucidum polysaccharide method according to claim 2 to 7 any one
It is prepared.
9. a kind of antineoplastic pharmaceutical compositions, wherein the antineoplastic pharmaceutical compositions contain described in a effective amount of claim 8
Plain boiled pork ganoderma lucidum polysaccharide.
10. antineoplastic pharmaceutical compositions according to claim 9, which is characterized in that the antineoplastic pharmaceutical compositions into
One step includes one or more pharmaceutically acceptable auxiliary materials.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094358A (en) * | 2020-09-22 | 2020-12-18 | 广东省微生物研究所（广东省微生物分析检测中心） | Tibetan ganoderma lucidum polysaccharide GLP-1 with antioxidant effect, and preparation method and application thereof |
| CN112569336A (en) * | 2020-12-16 | 2021-03-30 | 广东省微生物研究所（广东省微生物分析检测中心） | Application of grifola frondosa glycoprotein in preparation of drugs or health foods for enhancing body immunity and preventing and/or treating tumor diseases |
| CN113122458A (en) * | 2020-06-11 | 2021-07-16 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Ganoderma leucocontextum Z160097 and cultivation method and application thereof |
| CN113278535A (en) * | 2021-06-30 | 2021-08-20 | 四川省中医药科学院 | New ganoderma lucidum strain ZL167 and new application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113122458A (en) * | 2020-06-11 | 2021-07-16 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Ganoderma leucocontextum Z160097 and cultivation method and application thereof |
| CN112094358A (en) * | 2020-09-22 | 2020-12-18 | 广东省微生物研究所（广东省微生物分析检测中心） | Tibetan ganoderma lucidum polysaccharide GLP-1 with antioxidant effect, and preparation method and application thereof |
| WO2022062380A1 (en) * | 2020-09-22 | 2022-03-31 | 广东省微生物研究所（广东省微生物分析检测中心） | Tibetan ganoderma lucidum polysaccharide glp-1 with antioxidant effect, preparation method and use |
| CN112094358B (en) * | 2020-09-22 | 2022-05-27 | 广东省微生物研究所（广东省微生物分析检测中心） | Tibetan ganoderma lucidum polysaccharide GLP-1 with antioxidant effect, and preparation method and application thereof |
| CN112569336A (en) * | 2020-12-16 | 2021-03-30 | 广东省微生物研究所（广东省微生物分析检测中心） | Application of grifola frondosa glycoprotein in preparation of drugs or health foods for enhancing body immunity and preventing and/or treating tumor diseases |
| CN112569336B (en) * | 2020-12-16 | 2023-08-11 | 广东省微生物研究所（广东省微生物分析检测中心） | Application of russula cinerea glycoprotein in preparation of medicines or health foods for enhancing organism immunity and preventing and/or treating tumor diseases |
| CN113278535A (en) * | 2021-06-30 | 2021-08-20 | 四川省中医药科学院 | New ganoderma lucidum strain ZL167 and new application thereof |
| WO2023040033A1 (en) * | 2021-09-18 | 2023-03-23 | 广东粤微食用菌技术有限公司 | Anti-oxidant repair ganoderma lucidum extract and preparation method therefor |
| CN114766285A (en) * | 2022-04-24 | 2022-07-22 | 云南省农业科学院生物技术与种质资源研究所 | Ganoderma leucocontextum strain L4495 and cultivation method and application thereof |
| CN114766285B (en) * | 2022-04-24 | 2023-10-27 | 云南省农业科学院生物技术与种质资源研究所 | Ganoderma lucidum strain L4495 and cultivation method and application thereof |
| CN115886242A (en) * | 2022-11-23 | 2023-04-04 | 河北岁神本草肉灵芝生物科技有限公司 | Ganoderma lucidum, high-concentration oral liquid containing ganoderma lucidum and preparation method of oral liquid |
| CN116270712A (en) * | 2023-02-24 | 2023-06-23 | 安徽中医药大学 | Application of ganoderma lucidum polysaccharide based on white meat in preparation of antitumor drugs |
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