CN109276558A - Functionalized nano diamond drug-loading system and preparation method with targeting - Google Patents
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Abstract
The present invention relates to functionalized nano diamond drug-loading systems and preparation method with targeting.The present invention has that reference area is big, characteristic of strong adsorption using Nano diamond, hydrophobic anticancer drug and optothermal material are adsorbed on Nano diamond surface, the physical action to be attracted each other using positive and negative charge is in Nano diamond adsorption albumen with anti-tumor activity, improve the dispersibility of nano particle in water, and in outer layer covers targeting material, optothermal material is added in internal layer, improves drugloading rate to realize, hydrophobic anticancer drug targeted delivery to tumor locus and combination photo-thermal therapy are reinforced drug to the therapeutic effect of tumour.The present invention solves the problems, such as the burst release of hydrophobic anticancer medicine by easy-to-use method, also solves the problems, such as the active targeting of nano particle.
Description
Technical field
Nano-medicament carrier material field of the present invention, and in particular to there is one kind targeted nanometer to carry drug carrier and its preparation
Method.
Background technique
Curcumin is a kind of chemical component extracted from the rhizome of some plants in Zingiber, Araeceae, wherein ginger
Huang is the very rare pigment with diketone of plant kingdom containing about 3%~6%, is cyclohexadione compounds.Medicine shows that curcumin is logical
It crosses inducing malignant tumor cell differentiation, inducing apoptosis of tumour cell and its anticancer is played to the depression effect of tumour growth each phase
Effect, clinical application are very extensive.But the hydrophobic effect of curcumin and stability difference limit its application on drug.
Indocyanine green (indocyanine green, ICG) is a kind of typical colored cyanines class nir dye and the U.S.
FDA approval can be used for clinical optothermal material.The launch wavelength of ICG in 800nm or so, therefore bio-imaging etc. application in
It is relatively stable.The application of the photothermal conversion and photodynamic activity of ICG, more its photo-thermal therapy and optical dynamic therapy provides may
Property.
Hyaluronic acid (HA) molecular weight with higher and the ability for being detained a large amount of water, it is extracellular with other in space between cells
In matrix (collagen, elastomer) hyaluronic acid maintain one open wide, moisten and stable environment, serve as lubricant and
The effect of damper.Meanwhile hyaluronic acid is the cancer target ligand of HA receptor-specific, such as CD44, due to its good life
Object degradability, biocompatibility and non-immunogenic toxicity have been widely used for modification transmitting carrier.
Photo-thermal therapy is by the agent of conjunctive use near-infrared absorption and near-infrared stronger for tissue penetration power
Light increases local tissue temperature, so that cell degeneration, necrosis, a kind of green remedy for reaching therapeutic purposes.It is medium, warm
Heat treatment with non-lethal dose can make tumour cell become sensitive to radiotherapy and many drugs.Joint chemistry-photo-thermal therapy
The effect of chemotherapeutic can be enhanced to a certain extent.By introducing optothermal material, anticancer drug such as curcumin is made after heating
Toxicity greatly enhance, blood circulation accelerates after tumor locus temperature increases, and tumor tissues greatly increase the intake of drug.
The advantage of multifunctional nano drug-loading system is: first, reinforce suction of the Nano diamond to drug after modifying by nucleoprotamine
Appendix pharmacological property stability, and slow release can be played the role of.Second, by introducing optothermal material, chemistry-photo-thermal can be passed through
The toxicity of combination therapy reinforcement anticancer drug.Third is added hyaluronic acid, target-oriented drug can be improved.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of multifunctional nano drug-loading systems and preparation method thereof.This
Invention is contained hydrophobic anticancer medicine and optothermal material (ICG) in protein nucleoprotamine by molecular self-assembling method, and
The encapsulating of outer layer hyaluronic acid solves anticancer drug dissolution in combination with photo-thermal therapy to realize the targeted delivery of drug
Spend low and passive target and therapeutic effect difference problem.
In order to achieve the above object, the present invention adopts the following technical scheme that.
A kind of multifunctional nano drug-loading system, characterized in that it comprises: Nano diamond is carrier, is modified in nanometer
The protein molecule of diamond surface and the material that tumor surface can be targeted to；Load hydrophobic anticancer drug and photo-thermal material
Material；
The average grain diameter of the nano medicament carrying system is 116.4-128.5nm, dispersion degree 0.142-0.156, encapsulates
Rate is 90%-95%.
The material that tumor surface can be targeted to is selected from one or more of polysaccharide, folic acid, polyethylene glycol.
The optothermal material is selected from one or more of indocyanine green ICG, IR780, Prussian blue, carbon nano rod.
The method for preparing a kind of multifunctional nano drug-loading system, which comprises the steps of:
(1) hydrophobic anticancer medicine is dissolved in organic solvent, optothermal material is dissolved in deionized water, is then added to nanometer
In diamond dispersion liquid, the first mixed liquor is obtained；
(2) protein molecule is dissolved in the water, obtains the second mixed liquor；
(3) after mixing the first solution with the second solution, vortex centrifugal is centrifuged again after redissolving gained precipitating；It repeats
Aforesaid operations are dispersed in water to obtain the first dispersion liquid more than three times afterwards；
(4) material that will be targeted to tumor surface is soluble in water, obtains third solution；By third solution and first point
Dispersion liquid mixing, is centrifuged after stirring half an hour, collects precipitating；
Wherein material, protein molecule, Nano diamond and the hydrophobic anticancer medicine that tumor surface can be targeted to
Mass ratio with optothermal material is (500-40): (200-20): (20-2): (1-5): 1.
The organic solvent is selected from the one or more of methanol, acetonitrile or dimethyl sulfoxide.
The average grain diameter of the nano pesticide composition be 114-153.7nm, such as 108.6nm, 144.5nm,
165.3nm,181.7nm,116.4nm,130.8nm,；It is preferred that average grain diameter is 116.4-128.5nm；
The dispersion degree of the Nano medication composition is 0.142-0.272；It is preferred that dispersion degree is 0.142-0.156；
The encapsulation rate of the Nano medication composition is 90%-95%.It can be seen that the drugloading rate using this programme is very high, substantially
Realize full cladding, and the nano-scale after optimization is smaller, it is easier to be enriched in tumor locus.
The solubility of the hydrophobic anticancer medicine in water is less than 0.5g/1000g；Curcumin, taxol, five fluorine urine are phonetic
One of pyridine, camptothecine are a variety of, further preferred curcumin.
The bioprotein molecule is selected from one or more of nucleoprotamine, polypeptide；It is preferred that nucleoprotamine.
The present invention provides a kind of preparation method of multifunctional nano drug-loading system, comprising: by self-assembly method by hydrophobicity
Anticarcinogen and optothermal material contain in nucleoprotamine modification Nano diamond in, after wrapped up with hyaluronic acid.
Concrete operations are as follows:
(1) curcumin is dissolved in organic solvent, takes and is added in nanodiamond liquid dispersion in right amount, it is water-soluble that ICG is added
Liquid obtains the first mixed liquor；
(2) appropriate nucleoprotamine is dissolved in the water, obtains the second solution liquid；
(3) after mixing the first mixed solution with the second solution, vortex centrifugal is centrifuged again after redissolving gained precipitating.
Aforesaid operations are repeated to be dispersed in water to obtain the first dispersion liquid three times afterwards；
(4) hyaluronic acid is soluble in water, obtain third solution.Third solution is mixed with the first dispersion liquid, stirring half
It is centrifuged after hour, collects precipitating, obtain nano pesticide composition.
In step (1), every milliliter of organic solvent contains curcumin 100-1000 microgram, for example, 100 micrograms, 200 micrograms,
300 micrograms, 400 micrograms, 500 micrograms, 1000 micrograms etc., preferably every milliliter of organic solvent contain the curcumin 200-400 milli
Gram.
In step (1), every milliliter of water contains the indocyanine green 100-400 microgram, for example, 100 micrograms, 200 micrograms,
300 micrograms, 400 micrograms, 500 micrograms etc., preferably 100-200 milligrams, further preferably 200 milligrams.
In step (1), the nanodiamond liquid dispersion concentration is 0.5-10 milligrams, for example, 0.5 milligram, 1 milligram, 2
Milligram, 4 milligrams, 10 milligrams etc., 1 mg/ml.
In step (2), the nucleoprotamine concentration of aqueous solution be 1-20 milligrams, for example, 1 milligram, 5 milligrams, 10 milligrams,
15 milligrams, 20 milligrams etc., 10 mg/mls.
In step (4), every milliliter of water contains 1-50 milligrams of hyaluronic acid, for example, 5 milligrams, 10 milligrams, 15 milligrams, 20 millis
Gram, 30 milligrams, 40 milligrams, 50 milligrams etc., preferably 5-20 milligrams, further preferably 10-20 milligrams.
Preparation method is simple for multifunctional nano drug-loading system of the present invention, by by hydrophobic anticancer medicine medicine and
Optothermal material ICG is contained in the Nano diamond of nucleoprotamine functionalization, and outer layer covers have the hyaluronic acid of targeting,
Realize the targeted delivery of drug.The introducing of optothermal material also achieves the synergistic treatment of anticancer drug and photo-thermal therapy.It solves
The problems such as drug is degradable in tumor environment, burst release.
Detailed description of the invention
Fig. 1 is the image of the Nano medication composition obtained in bioelectricity microscopic observation embodiment 1.
Fig. 2 is the image that the Nano medication composition that embodiment 1 obtains is observed under scanning electron microscope.
Fig. 3 is the grain-size graph that laser particle analyzer measures the Nano medication composition that embodiment 1 obtains.
Fig. 4 is the potential diagram that laser particle analyzer measures the Nano medication composition that embodiment 1 obtains.
Fig. 5 is the in vitro toxicity lab diagram of drug-loading nanoparticles and raw medicine to HepG-2 cell.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
In the present invention, the volume of gas and liquid is the numerical value of normal atmosphere pressure at 20 DEG C.Nano diamond
The partial size of dispersion liquid is 50~60nm.
Embodiment 1
Steps are as follows:
(1) 2 milligrams of curcumin is dissolved in 1mL methanol, 2 milligrams of ICG are dissolved in 1mL deionized water, respectively take 100 microlitres
It is added in the nanodiamond liquid dispersion of 1 mg/ml and obtains the first mixed liquor.
(2) 10 milligrams of nucleoprotamine (protamine sulfate PS is purchased from Sigma) is dissolved in 1mL deionized water, is obtained
To the second solution
(3) the first mixed solution is added in the second solution, is vortexed 1 minute, after centrifugation plus deionized water is redissolved.It is centrifuged again
Add water to redissolve, repeats above operation 3 times.Obtaining stable dispersion liquid is the first dispersion liquid.
(4) 20 milligrams of hyaluronic acids are dissolved in 1 ml deionized water, obtain third solution.By the first dispersion liquid and
The mixing of three solution, is stirred 30 minutes, is centrifuged and is collected precipitating to get stable nano particle is arrived.
Testing result:
According to described in document (Qing Xu is waited biomaterial (Biomaterials), 2012,33,1627-1639)
Method is in cluster state, particle size is about in the nanometer drug composition (see Fig. 1) that bioelectricity microscopic observation the present embodiment obtains
For 130.8nm.
According to described in document (Qing Xu is waited biomaterial (Biomaterials), 2012,33,1627-1639)
Method observes the nanometer drug composition (see Fig. 2) that the present embodiment obtains, at cluster-shaped state under scanning electron microscope.
According to described in document (Qing Xu is waited biomaterial (Biomaterials), 2012,33,1627-1639)
Method is 130.4 ± 2nm (see Fig. 3) using the partial size that laser particle analyzer measures the nanometer drug composition that the present embodiment obtains, point
Divergence is 0.156.
According to described in document (Qing Xu is waited biomaterial (Biomaterials), 2012,33,1627-1639)
Method is -21.3mV using the current potential that laser particle analyzer measures the nanometer drug composition that the present embodiment obtains.
Comparative example 1
The present embodiment prepares Nano medication composition in the same manner as shown in Example 1, the difference is that being added
Hyaluronic acid contents are 0 milligram in three solution.
According to measurement method same as Example 1, the nano pesticide that the present embodiment obtains is measured using laser particle analyzer
The partial size of composition is 115.7nm；Dispersion degree is 0.182, and measuring surface potential is+32mV.
Comparative example 2
The present embodiment prepares Nano medication composition in the same manner as shown in Example 1, the difference is that ginger be added
Flavine and ICG are 0mg.
According to measurement method same as Example 1, the nano pesticide that the present embodiment obtains is measured using laser particle analyzer
The partial size of composition is 122.3nm；Dispersion degree is 0.091, and measuring surface potential is -24.6mV.
Embodiment 3
The present embodiment prepares Nano medication composition in the same manner as shown in Example 1, the difference is that ginger be added
Flavine is 50 microlitres.
According to measurement method same as Example 1, the nanometer medicine group that the present embodiment obtains is measured using laser particle analyzer
The partial size for closing object is 108.6nm；Dispersion degree is 0.142, and measuring surface potential is -11.3mV, encapsulation rate 95%
Embodiment 4
The present embodiment prepares a nanometer drug composition in the same manner as shown in Example 1, the difference is that turmeric be added
Element is 200 microlitres.
According to measurement method same as Example 1, the nanometer medicine group that the present embodiment obtains is measured using laser particle analyzer
The partial size for closing object is 165.3nm；Dispersion degree be 0.170 measure surface potential be -15.1mV, encapsulation rate 92%
Embodiment 5
The present embodiment prepares nano pesticide composition in the same manner as shown in Example 1, the difference is that ginger be added
Flavine is 400 microlitres.
According to measurement method same as Example 1, the nanometer medicine group that the present embodiment obtains is measured using laser particle analyzer
The partial size for closing object is 181.7nm；Dispersion degree is 0.272, and measuring surface potential is -14.9mV, encapsulation rate 90%.
Embodiment 6
The present embodiment prepares nano pesticide composition in the same manner as shown in Example 1, the difference is that the third
Hyaluronic acid contents are 5 milligrams in solution.
According to measurement method same as Example 1, the nanometer medicine group that the present embodiment obtains is measured using laser particle analyzer
The partial size for closing object is 116.4nm；Dispersion degree is 0.225；Measuring surface potential is -11.9mV, encapsulation rate 92%
Embodiment 7
The present embodiment prepares nano pesticide composition in the same manner as shown in Example 1, the difference is that the third
Hyaluronic acid contents are 10 milligrams in solution.
According to measurement method same as Example 1, the nanometer medicine group that the present embodiment obtains is measured using laser particle analyzer
The partial size for closing object is 144.5nm；Dispersion degree is 0.176；Measuring surface potential is -13.6mV, encapsulation rate 92%
It is obtained by above-described embodiment as drawn a conclusion:
(1) by adjusting Nano diamond, the mass ratio of curcumin, determine that Nano diamond contains the best matter of curcumin
Amount ratio is (10-2): 1.
(2) under the premise of material gross mass is certain, with the increase of dosage, partial size and PDI are increased
Increase.
(3) by changing the input amount of hyaluronic acid, available surface is on behalf of different nano particles.So that anticarcinogen
When in use, particle is more stable for object.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (7)
1. a kind of multifunctional nano drug-loading system, characterized in that it comprises: Nano diamond is carrier, is modified in nanogold
The protein molecule on hard rock surface and the material that tumor surface can be targeted to；Load hydrophobic anticancer drug and optothermal material；
The average grain diameter of the nano medicament carrying system is 116.4-128.5nm, dispersion degree 0.142-0.156, encapsulation rate are
90%-95%.
2. nano medicament carrying system according to claim 1, which is characterized in that the protein molecule be selected from nucleoprotamine,
One or more of polypeptide.
3. nano medicament carrying system according to claim 1, which is characterized in that the material that tumor surface can be targeted to
Selected from one or more of polysaccharide, folic acid, polyethylene glycol.
4. nano medicament carrying system according to claim 1, which is characterized in that the optothermal material be selected from indocyanine green ICG,
One or more of IR780, Prussian blue, carbon nano rod.
5. nano medicament carrying system according to claim 1, which is characterized in that the dissolution of the hydrophobic anticancer medicine in water
Degree is less than 0.5g/1000g.
6. preparing a kind of method of multifunctional nano drug-loading system as described in claim 1, which is characterized in that including walking as follows
It is rapid:
(1) hydrophobic anticancer medicine is dissolved in organic solvent, optothermal material is dissolved in deionized water, is then added to a nanometer Buddha's warrior attendant
In stone dispersion liquid, the first mixed liquor is obtained；
(2) protein molecule is dissolved in the water, obtains the second mixed liquor；
(3) after mixing the first solution with the second solution, vortex centrifugal is centrifuged again after redissolving gained precipitating；It repeats above-mentioned
Operation is dispersed in water to obtain the first dispersion liquid more than three times afterwards；
(4) material that will be targeted to tumor surface is soluble in water, obtains third solution；By third solution and the first dispersion liquid
Mixing is centrifuged after stirring half an hour, collects precipitating；
Wherein material, protein molecule, Nano diamond and the hydrophobic anticancer medicine and light that tumor surface can be targeted to
The mass ratio of hot material is (500-40): (200-20): (20-2): (1-5): 1.
7. preparation method according to claim 6, it is characterised in that: in step (1), the organic solvent be selected from methanol,
Acetonitrile or dimethyl sulfoxide it is one or more.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110882398A (en) * | 2019-12-24 | 2020-03-17 | 辽宁科技学院 | Oral curcumin-nano diamond compound and preparation method thereof |
| EP3939612A4 (en) * | 2019-03-13 | 2023-03-29 | National Institute Of Advanced Industrial Science And Technology | Optically heat-generating composite material, nanocluster, substance delivery carrier and pharmaceutical composition |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102459064A (en) * | 2009-05-28 | 2012-05-16 | 西北大学 | Nano-diamond particle complexes |
| WO2013068625A1 (en) * | 2011-11-11 | 2013-05-16 | Universidade De Santiago De Compostela | Nanoparticulate systems prepared from sorbitan esters |
| CN103849652A (en) * | 2013-12-22 | 2014-06-11 | 北京工业大学 | Nano carrier compound for microRNA (Ribonucleic Acid) targeting delivery and preparation method and application thereof |
| WO2014087413A1 (en) * | 2012-12-03 | 2014-06-12 | Vishwa Vidya Peetham University Amrita | Nanoparticles comprising sorafenib |
| CN104784703A (en) * | 2015-04-20 | 2015-07-22 | 北京工业大学 | Aptamer-based targeted delivery microRNA nanometer carrier as well as preparation method and application thereof |
-
2018
- 2018-09-19 CN CN201811091205.XA patent/CN109276558A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102459064A (en) * | 2009-05-28 | 2012-05-16 | 西北大学 | Nano-diamond particle complexes |
| WO2013068625A1 (en) * | 2011-11-11 | 2013-05-16 | Universidade De Santiago De Compostela | Nanoparticulate systems prepared from sorbitan esters |
| WO2014087413A1 (en) * | 2012-12-03 | 2014-06-12 | Vishwa Vidya Peetham University Amrita | Nanoparticles comprising sorafenib |
| CN103849652A (en) * | 2013-12-22 | 2014-06-11 | 北京工业大学 | Nano carrier compound for microRNA (Ribonucleic Acid) targeting delivery and preparation method and application thereof |
| CN104784703A (en) * | 2015-04-20 | 2015-07-22 | 北京工业大学 | Aptamer-based targeted delivery microRNA nanometer carrier as well as preparation method and application thereof |
Non-Patent Citations (7)
| Title |
|---|
| BARBARA STROJNY等: "Diamond Nanoparticles Modify Curcumin", 《PLOS ONE》 * |
| CAIYAN ZHAO等: "Photosensitive Nanoparticles Combining Vascular-Independent", 《ADVANCED SCIENCE NEWS》 * |
| MINJUN CAO等: "Protamine sulfate–nanodiamond hybrid nanoparticles", 《NANOSCALE》 * |
| QIAN CHEN等: "An Imagable and Photothermal "Abraxane-Like" Nanodrug for Combination Cancer Therapy to Treat Subcutaneous and Metastatic Breast Tumors", 《ADVANCED MATERIALS》 * |
| SHIHUA WANG等: "Degradable Hyaluronic Acid/Protamine Sulfate", 《ADVANCED HEALTHCARE MATERIALS》 * |
| 曹敏军: "新型纳米技术介导的 miRNA 的靶向递送及其在肿瘤治疗中的应用", 《中国博士学位论文全文数据库》 * |
| 钟冬望等: "《爆炸技术新进展》", 31 October 2002, 湖北科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3939612A4 (en) * | 2019-03-13 | 2023-03-29 | National Institute Of Advanced Industrial Science And Technology | Optically heat-generating composite material, nanocluster, substance delivery carrier and pharmaceutical composition |
| CN110882398A (en) * | 2019-12-24 | 2020-03-17 | 辽宁科技学院 | Oral curcumin-nano diamond compound and preparation method thereof |
| CN110882398B (en) * | 2019-12-24 | 2022-11-08 | 辽宁科技学院 | Oral curcumin-nano diamond compound and preparation method thereof |
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Application publication date: 20190129 |