CN109134015A - A kind of compost maturity additive and its application - Google Patents
A kind of compost maturity additive and its application Download PDFInfo
- Publication number
- CN109134015A CN109134015A CN201810911355.4A CN201810911355A CN109134015A CN 109134015 A CN109134015 A CN 109134015A CN 201810911355 A CN201810911355 A CN 201810911355A CN 109134015 A CN109134015 A CN 109134015A
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- compost
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- seed liquor
- additive
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to a kind of compost maturity additives, according to the mass fraction, raw material by include 100 parts of fresh beer vinasse, 5-15 parts of wheat bran, 2-4 parts of ammonium sulfate, 10-20 parts of urea, 0.5-1 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of NaCl；Volume total amount is the strain seed liquor of the 10-20%v/w of fresh beer vinasse quality；Wherein strain seed liquor is aspergillus niger, and Penicillium notatum, Ralstonia bacterium and candida utili volume ratio are 2~3:2~3:1~2:1~2 seed liquid mixture.The present invention be rationally utilized fresh beer vinasse as culture medium the initial fermentation under the synbiosis of multiple-microorganism, both the quantity of bacterium had been expanded, cellulase further progress decomposition of cellulose is generated again, multi cellulose enzyme collaboration simultaneously makees that effective degradation of cellulose can also be improved, and preferably promotes the organic decomposition of poultry waste.
Description
Technical field
The invention belongs to field of biotechnology, it is related to a kind of compost maturity additive and its application.
Background technique
With the rapid development of China's scale animal and poultry cultivation industry, a large amount of feces of livestock and poultry have become new pollution sources.High temperature
Aerobic compost is feces of livestock and poultry harmless treatment and the important channel that Fertilizer Transformed utilizes.The general fermentation period of Traditional compost is longer,
Low efficiency, to accelerate compost maturity process, people often pre-process composting material, in preprocessing process, using most
More methods is that microorganism formulation is added into material.It is reported that China production microbial bacterial agent producer more than 2000,
But different composite microbial inoculum, different addition auxiliary materials and proportion cause compost maturity effect different, and directly add microorganism system
Agent, needing to add a large amount of bacterial strain can be only achieved effect.
Brewex's grains be produce the primary raw material rice of beer, malt etc. be filtered through gelatinization, Mashing process or filters pressing it
Remaining solid matter afterwards, main component include protein, fibre fractionation, vitamin, fat, minerals and starch etc., so
The nutritive value of brewex's grains is relatively high, and crude protein is up to 25% or more.There are many raisers to take away when feed is fine, but because of nutrition
Ingredient is preferable, and water content is big, very apt to deteriorate rancid, sometimes can also resolve into pectic acid and first during the fermentation
Aldehyde, these things are all the substances for having injury to human body and livestock and poultry, and livestock and poultry, which have eaten to have loose bowels instead, falls ill.Enterprises many in this way can only
It as waste discharge, not only results in waste of resources, or even generates serious environmental pollution.
Summary of the invention
In view of this, the purpose of the present invention is to provide it is a kind of make full use of brewex's grains prepare compost maturity additive and
It is applied.
In order to achieve the above objectives, the invention provides the following technical scheme:
1. a kind of compost maturity additive, the raw material of the additive according to the mass fraction, by following material composition: fresh beer
100 parts of vinasse, 5-15 parts of wheat bran, 2-4 parts of ammonium sulfate, 10-20 parts of urea, 0.5-1 parts of potassium dihydrogen phosphate, NaCl 0.1-0.5
Part；Volume total amount is the strain seed liquor of the 10-20%v/w of fresh beer vinasse quality；The strain seed liquor is aspergillus niger, mould
Bacterium, Ralstonia bacterium and candida utili volume ratio are 2~3:2~3:1~2:1~2 seed liquid mixture.
Further, strain seed liquor volume total amount is the 15%v/w of fresh beer vinasse quality.
Further, the seed liquor miospore number of the aspergillus niger is 2~2.5 × 108CFU/ml；The seed of the Penicillium notatum
Liquid miospore number is 2~2.5 × 108CFU/ml。
Further, bacteria concentration is 1~1.5 × 10 in the seed liquor of the Ralstonia bacterium8CFU/ml。
Further, bacteria concentration is 1.5~2 × 10 in the seed liquor of the candida utili8CFU/ml。
2. the application of compost maturity additive of the invention in compost production.
3. adding the compost of compost maturity additive of the invention, the compost contains compost maturity additive, animal dung
Dirty and carbons organic waste.
Further, the carbon-nitrogen ratio of the livestock and poultry feces and carbons organic waste is 20-35, the matter of compost maturity additive
Amount is 1:2.5-4 than the ratio of livestock and poultry feces and carbons organic waste quality sum.
Further, carbons organic waste is soybean stalk, bagasse, wheat-straw, rice straw, wheat skin, skin of beancurd and/or stalk.
The beneficial effects of the present invention are: symbiosis of the fresh beer vinasse as culture medium in multiple-microorganism is rationally utilized and makees
With lower initial fermentation, the quantity of bacterium had not only been expanded, but also has generated cellulase further progress decomposition of cellulose, while multiple fiber
Plain enzyme collaboration makees that effective degradation of cellulose can also be improved.Added aspergillus niger, Penicillium notatum, Ralstonia bacterium and production protein
Candida reaches good symbiosis conditions, phase under the reasonable culture medium based on fresh beer vinasse, supplemented by other inorganic elements
Mutually promote breeding, is in composting process, labile organic matter (such as starch, carbohydrate) and more labile organic matter are (such as fine
Dimension element etc.) it lays the foundation.And reduce the dosage of bacterial strain seed liquor with the inventive method, more convenient for industrialization largely into
Row compost fermentation.
Specific embodiment
A preferred embodiment of the present invention will be described in detail below.The experiment side of actual conditions is not specified in embodiment
Method, usually according to conventional conditions or according to the manufacturer's recommendations.
Embodiment 1
Aspergillus niger is inoculated in PDA culture medium, 30 DEG C, with 220~250r/min culture 72 hours, reaches 2 with spore count
~2.5 × 108CFU/ml, as mature seed liquor.
Penicillium notatum is inoculated in PDA culture medium, 30 DEG C, with 220~250r/min culture 72 hours, reaches 2 with spore count
~2.5 × 108CFU/ml, as mature seed liquor.
Ralstonia bacterium (Ralstonia sp., Shanghai Ke Wei chemical technology Co., Ltd) culture medium: peptone
0.5%, beef leaches object 0.3%, magnesium sulfate 0.5-1%, NaCl 0.5%, agar 1.5%, pH7.0,220~250r/min
Culture 48-72 hours, reaches 1 × 10 with bacteria concentration8CFU/ml, as mature seed liquor.
Candida utili (purchase is in Chinese industrial Culture Collection) uses culture medium: peptone 2-3%, Portugal
Grape sugar 5%, potassium dihydrogen phosphate 0.5-1%, magnesium sulfate 0.1%, ammonium sulfate 0.5%, pH 5.5 are cultivated with 220~250r/min,
12~24 hours, 1.5-2 × 10 are reached with bacteria concentration8CFU/ml, as mature seed liquor.
Embodiment 2
Will in every 1000 grams of the quality of fresh beer vinasse be added 100 grams of wheat bran, 30 grams of ammonium sulfate, 15 grams of urea, biphosphate
10 grams of potassium, 5 grams of NaCl；Above-mentioned kind is successively inoculated with by 15% that inoculation liquid volume total amount (V) is fresh beer vinasse quality (w) again
Sub- liquid, wherein aspergillus niger 45ml, Penicillium notatum 45ml, Ralstonia bacterium 30ml and candida utili 30ml；The 48-72 that ferments is small
When.
Embodiment 3
Will in every 1000 grams of the quality of fresh beer vinasse be added 50 grams of wheat bran, 20 grams of ammonium sulfate, 10 grams of urea, potassium dihydrogen phosphate
8 grams, 3 grams of NaCl；Above-mentioned seed is successively inoculated with by 18% that inoculation liquid volume total amount (V) is fresh beer vinasse quality (w) again
Liquid, wherein aspergillus niger 40ml, Penicillium notatum 40ml, Ralstonia bacterium 20ml and candida utili 20ml；Fermentation 48-72 hours
?.
Embodiment 4
Will in every 1000 grams of the quality of fresh beer vinasse be added 100 grams of wheat bran, 40 grams of ammonium sulfate, 20 grams of urea, biphosphate
8 grams of potassium, 3 grams of NaCl；Above-mentioned seed is successively inoculated with by 12% that inoculation liquid volume total amount (V) is fresh beer vinasse quality (w) again
Liquid, wherein aspergillus niger 60ml, Penicillium notatum 60ml, Ralstonia bacterium 30ml and candida utili 30ml；Fermentation 48-72 hours
?.
Embodiment 5
After 80kg fowl and animal excrement and the 10kg stalk of crushing are mixed, the carbon-nitrogen ratio for adjusting composting material is 30~40, water
Dividing content is 50~60%, adds the brewex's grains 30kg that the fermentation of embodiment 1 finishes, mixes well rear static fermentation；To 2-3 days
Temperature fermentation is to 55-60 DEG C of progress turning, then ferments 15-20 days to completely decomposed.If using having fermentation in the prior art
If the compost reactor of slot carries out compost, reach the complete decomposed decomposed time being substantially shorter.
Embodiment 6
After 80kg fowl and animal excrement and the 10kg stalk of crushing are mixed, the carbon-nitrogen ratio for adjusting composting material is 32, and moisture contains
Amount is 50~60%, adds the brewex's grains 30kg that the fermentation of embodiment 1 finishes, mixes well rear static fermentation；To 2-3 days temperature
Fermentation is to 55-60 DEG C of progresss turning, then ferments 15-20 days to complete decomposed.
Embodiment 7
After 80kg fowl and animal excrement and the 5kg wheat-straw of crushing, 5kg rice straw, 5kg stalk are mixed, composting material is adjusted
Carbon-nitrogen ratio is 35, and moisture content is 50~60%, adds the brewex's grains 35kg that the fermentation of embodiment 2 finishes, mixes well rear quiet
Only ferment；To 2-3 days temperature fermentations to 55-60 DEG C of progress turning, then ferment 15-20 days to completely it is decomposed.
Embodiment 8
After 80kg fowl and animal excrement and the 20kg bagasse of crushing are mixed, the carbon-nitrogen ratio for adjusting composting material is 35, and moisture contains
Amount is 50~60%, adds the brewex's grains 30kg that the fermentation of embodiment 1 finishes, mixes well rear static fermentation；To 2-3 days temperature
Fermentation is to 55-60 DEG C of progresss turning, then ferments 15-20 days to complete decomposed.
9 seed germination index of embodiment
The measurement of seed germination index (germination index, GI): sample and distilled water are sufficiently mixed in 1:10 ratio
It closes, extract 24 hours at 30 DEG C, filter, filtrate is centrifuged 10min with 3 000r/min, and absorption 5mL supernatant is in being covered with filter paper
In culture dish, 20 mung bean seeds are placed, use distilled water as control, 5 repetitions, 25 ± 1 DEG C of culture 2d calculate the hair of seed
Bud rate, and with the root long of vernier caliper measurement seed.According to following equation, the germination index (GI) of seed is calculated:
GI (%)=[(compost extraction percentage of seedgermination × seed root long)/(distilled water percentage of seedgermination × seminal root
It is long)] × 100%.
Kjeldahl method；Organic carbon uses potassium dichromate oxidation.Embodiment 7-9 sample tests such as table
Shown in 1.
The germination index of each embodiment sample of table 1
| Sample | Germination percentage | Germination index |
| Embodiment 7 | 96.67% | 93.4% |
| Embodiment 8 | 95% | 85.7% |
| Embodiment 9 | 96.67% | 91.2% |
| Common compost | 90.0% | 74.6% |
| Control | 85% |
It is decomposed to illustrate that compost prepared by compost maturity additive of the invention has reached, and does not have phytotoxicity.
Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (9)
1. a kind of compost maturity additive, which is characterized in that the raw material of the additive according to the mass fraction, by following substance group
At: 100 parts of fresh beer vinasse, 5-15 parts of wheat bran, 2-4 parts of ammonium sulfate, 10-20 parts of urea, 0.5-1 parts of potassium dihydrogen phosphate, NaCl
0.1-0.5 parts；Volume total amount is the strain seed liquor of the 10-20%v/w of fresh beer vinasse quality；The strain seed liquor is black song
Mould, Penicillium notatum, Ralstonia bacterium and candida utili volume ratio are the mixing of 2~3:2~3:1~2:1~2 seed liquor
Object.
2. compost maturity additive according to claim 1, which is characterized in that strain seed liquor volume total amount is draft beer
The 15%v/w of poor quality.
3. compost maturity additive according to claim 1, which is characterized in that the seed liquor miospore number of the aspergillus niger
It is 2~2.5 × 108CFU/ml；The seed liquor miospore number of the Penicillium notatum is 2~2.5 × 108CFU/ml。
4. compost maturity additive according to claim 1, which is characterized in that in the seed liquor of the Ralstonia bacterium
Bacteria concentration is 1~1.5 × 108CFU/ml。
5. compost maturity additive according to claim 1, which is characterized in that in the seed liquor of the candida utili
Bacteria concentration is 1.5~2 × 108CFU/ml。
6. application of described in any item compost maturity additives in compost production according to claim 1~5.
7. adding the compost of the described in any item compost maturity additives of Claims 1 to 5, which is characterized in that the compost contains
There are compost maturity additive, livestock and poultry feces and carbons organic waste.
8. compost according to claim 7, which is characterized in that the carbon-nitrogen ratio of the livestock and poultry feces and carbons organic waste
For 20-35, the quality of compost maturity additive is 1:2.5- than the ratio of livestock and poultry feces and carbons organic waste quality sum
4。
9. compost according to claim 7, which is characterized in that carbons organic waste be soybean stalk, bagasse, wheat-straw,
Rice straw, wheat skin, skin of beancurd and/or stalk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810911355.4A CN109134015A (en) | 2018-08-11 | 2018-08-11 | A kind of compost maturity additive and its application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810911355.4A CN109134015A (en) | 2018-08-11 | 2018-08-11 | A kind of compost maturity additive and its application |
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| CN109134015A true CN109134015A (en) | 2019-01-04 |
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| CN201810911355.4A Pending CN109134015A (en) | 2018-08-11 | 2018-08-11 | A kind of compost maturity additive and its application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111410569A (en) * | 2020-04-22 | 2020-07-14 | 江苏食品药品职业技术学院 | Biological composting method for garden waste |
| CN113122483A (en) * | 2021-05-31 | 2021-07-16 | 哈尔滨工业大学 | Rapid heat-producing microbial inoculum for aerobic composting of corn straws and application of rapid heat-producing microbial inoculum |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0874790A1 (en) * | 1996-01-18 | 1998-11-04 | Miroslav Hofschneider | Fertilizer |
| CN105272395A (en) * | 2015-11-02 | 2016-01-27 | 仲恺农业工程学院 | Compound microbial preparation for quickly degrading cassava residues and preparation method and application thereof |
| CN105316243A (en) * | 2015-11-23 | 2016-02-10 | 鲁东大学 | Preparing method and application of composite biological agent for agricultural root-knot nematodes |
| CN105924246A (en) * | 2016-04-25 | 2016-09-07 | 上海交通大学 | Preparation method for biological fermentation integrated organic fertilizer |
-
2018
- 2018-08-11 CN CN201810911355.4A patent/CN109134015A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0874790A1 (en) * | 1996-01-18 | 1998-11-04 | Miroslav Hofschneider | Fertilizer |
| CN105272395A (en) * | 2015-11-02 | 2016-01-27 | 仲恺农业工程学院 | Compound microbial preparation for quickly degrading cassava residues and preparation method and application thereof |
| CN105316243A (en) * | 2015-11-23 | 2016-02-10 | 鲁东大学 | Preparing method and application of composite biological agent for agricultural root-knot nematodes |
| CN105924246A (en) * | 2016-04-25 | 2016-09-07 | 上海交通大学 | Preparation method for biological fermentation integrated organic fertilizer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111410569A (en) * | 2020-04-22 | 2020-07-14 | 江苏食品药品职业技术学院 | Biological composting method for garden waste |
| CN113122483A (en) * | 2021-05-31 | 2021-07-16 | 哈尔滨工业大学 | Rapid heat-producing microbial inoculum for aerobic composting of corn straws and application of rapid heat-producing microbial inoculum |
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Application publication date: 20190104 |