CN109045362A - Collagen microparticle and its preparation method and application - Google Patents
Collagen microparticle and its preparation method and application Download PDFInfo
- Publication number
- CN109045362A CN109045362A CN201811057546.5A CN201811057546A CN109045362A CN 109045362 A CN109045362 A CN 109045362A CN 201811057546 A CN201811057546 A CN 201811057546A CN 109045362 A CN109045362 A CN 109045362A
- Authority
- CN
- China
- Prior art keywords
- microparticle
- collagen
- preparation
- drying device
- rotary spraying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Biophysics (AREA)
- Cosmetics (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention relates to collagen microparticles and its preparation method and application, preparation method includes the following steps: by the rotary spraying and drying device to spray collagen solution with the state of microparticle, after the microparticle encounters the hot-air that temperature is 38 DEG C~53 DEG C during decline, being mixed in moisture therein can be constantly evaporated and by the gas outlet discharge above the rotary spraying and drying device；The micro mist for falling on the drying regime of tower bottom is blown afloat with hot wind, collides and is sticked together with the moist microparticle in dropping process；By outside the microparticle output tower after adhesion, obtaining partial size is 30 mesh~50 mesh collagen microparticle.The preparation method of collagen microparticle of the invention so that dispersibility increases substantially by the collagen protein powder mobility after the technique and in water, and reduces the dust raising phenomenon in process, shortens dissolution time by improving technique.
Description
Technical field
The present invention relates to a kind of collagen microparticles and its preparation method and application.
Background technique
Collagen is the most abundant protein of animal in-vivo content, be tendon, cartilage, skin and blood vessel it is main at
Point, account for the 25%~30% of total protein.Collagen has unique triple helices structure, and molecular structure is highly stable,
And there is low immunogenicity and good biocompatibility.Collagen is non-degradable in water, weak acid and weak base, and is boiling
Become the gelatin of soluble single-stranded structure afterwards, but since the molecular weight of collagen is bigger than gelatin, there is higher bioactivity,
Therefore, collagen is more suitable for biological tissue's utilization, is easier to promote regeneration of damaged tissue, shortens agglutination.Collagen mentions
After taking out, since its molecular weight is smaller, only 1000 dalton~3000 dalton, viscosity is very low, so that preparation
Protein particulate it is very small, cause to generate dust raising phenomenon in process, and in water dispersibility it is also not high.
Summary of the invention
An object of the present invention is to provide a kind of preparation methods of collagen microparticle.
The preparation method of collagen microparticle of the invention, includes the following steps: S101: setting rotating spraying first is dry
The temperature of dry device be 38 DEG C~53 DEG C, then by collagen solution by the rotary spraying and drying device with the state of microparticle
It sprays, after the microparticle encounters the hot-air that temperature is 38 DEG C~53 DEG C during decline, is mixed in moisture meeting therein
It is constantly evaporated and by the gas outlet discharge above the rotary spraying and drying device；S102: tower bottom will be fallen on hot wind
The micro mist of drying regime is blown afloat, and is collided and is sticked together with the moist microparticle in dropping process；S103: after adhesion
Microparticle output tower outside, obtain partial size be 30 mesh~50 mesh collagen microparticle.
The preparation method of collagen microparticle of the invention, by improving technique, allow it is dry after powder by hot wind with
The collagen protein powder being not yet completely dried occurs conducting repeated adhesion continuous cropping and uses, and eventually becomes porous loose microparticle structure, so that logical
Collagen protein powder mobility after crossing the technique and dispersibility increases substantially in water, and reduce the fugitive dust in process
Phenomenon shortens dissolution time.Any adhesive is not added, is bonded merely with the viscosity of collagen itself, makes to make
Product out also has purity is high, no added feature.
In addition, the preparation method of above-mentioned collagen microparticle of the invention, can also have following additional technology special
Sign:
Further, in the step S101, the collagen solution mass concentration is 28%~32%.
Further, in the step S102, the temperature of the hot wind is 28 DEG C~40 DEG C.
Further, in the step S102, the wind speed of the hot wind of tower bottom is 28km/h~38km/h.
Further, in the step S102, after the step S102 continues 5s~10s, then step S103 is carried out.
It is another object of the present invention to the collagen microparticles for proposing that the preparation method obtains.
Another object of the present invention is to propose that the method is preparing the application in collagen product.
Additional aspect and advantage of the invention will be set forth in part in the description, and will partially become from the following description
Obviously, or practice through the invention is recognized.
Specific embodiment
The embodiment of the present invention is described below in detail, the embodiment is exemplary, it is intended to it is used to explain the present invention, and
It is not considered as limiting the invention.
Embodiment 1
Embodiment 1 proposes a kind of collagen microparticle, and preparation method includes the following steps:
(1) temperature that rotary spraying and drying device is arranged first is 38 DEG C, the collagen for being then 32% by mass concentration
Solution is sprayed by the rotary spraying and drying device with the state of microparticle, when the microparticle encounters temperature during decline
After 38 DEG C of hot-airs, being mixed in moisture therein can be constantly evaporated and by going out above the rotary spraying and drying device
Port discharge.
(2) micro mist for falling on the drying regime of tower bottom is blown afloat with the hot wind that temperature is 40 DEG C, speed is 28km/h, with place
Moist microparticle in dropping process is collided and is sticked together.
(3) after the step (2) continue 10s, by outside the microparticle output tower after adhesion, the glue that partial size is 30 mesh is obtained
Former albumen microparticle.
Embodiment 2
Embodiment 2 proposes a kind of collagen microparticle, and preparation method includes the following steps:
(1) temperature that rotary spraying and drying device is arranged first is 53 DEG C, the collagen for being then 28% by mass concentration
Solution is sprayed by the rotary spraying and drying device with the state of microparticle, when the microparticle encounters temperature during decline
After 53 DEG C of hot-airs, being mixed in moisture therein can be constantly evaporated and by going out above the rotary spraying and drying device
Port discharge.
(2) micro mist for falling on the drying regime of tower bottom is blown afloat with the hot wind that temperature is 28 DEG C, speed is 38km/h, with place
Moist microparticle in dropping process is collided and is sticked together.
(3) after the step (2) continue 5s, by outside the microparticle output tower after adhesion, obtaining partial size is 30 mesh~50
Purpose collagen microparticle.
Embodiment 3
Embodiment 3 proposes a kind of collagen microparticle, and preparation method includes the following steps:
(1) temperature that rotary spraying and drying device is arranged first is 45 DEG C, the collagen for being then 30% by mass concentration
Solution is sprayed by the rotary spraying and drying device with the state of microparticle, when the microparticle encounters temperature during decline
After 45 DEG C of hot-airs, being mixed in moisture therein can be constantly evaporated and by going out above the rotary spraying and drying device
Port discharge.
(2) micro mist for falling on the drying regime of tower bottom is blown afloat with the hot wind that temperature is 36 DEG C, speed is 33km/h, with place
Moist microparticle in dropping process is collided and is sticked together.
(3) after the step (2) continue 8s, by outside the microparticle output tower after adhesion, the glue that partial size is 40 mesh is obtained
Former albumen microparticle.
The preparation method of collagen microparticle of the invention, by improving technique, allow it is dry after powder by hot wind with
The collagen protein powder being not yet completely dried occurs conducting repeated adhesion continuous cropping and uses, and eventually becomes porous loose microparticle structure, so that logical
Collagen protein powder mobility after crossing the technique and dispersibility increases substantially in water, and reduce the fugitive dust in process
Phenomenon shortens dissolution time.Any adhesive is not added, is bonded merely with the viscosity of collagen itself, makes to make
Product out also has purity is high, no added feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not
It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office
It can be combined in any suitable manner in one or more embodiment or examples.In addition, without conflicting with each other, the skill of this field
Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples
It closes and combines.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, modifies, replacement and variant.
Claims (7)
1. a kind of preparation method of collagen microparticle, which comprises the steps of:
S101: the temperature of setting rotary spraying and drying device first is 38 DEG C~53 DEG C, is then passed through collagen solution described
Rotary spraying and drying device is sprayed with the state of microparticle, is 38 DEG C~53 DEG C when the microparticle encounters temperature during decline
Hot-air after, being mixed in moisture therein can constantly be evaporated and by the gas outlet row above the rotary spraying and drying device
Out；
S102: being blown afloat the micro mist for falling on the drying regime of tower bottom with hot wind, is touched with the moist microparticle in dropping process
It hits and is sticked together；
S103: by outside the microparticle output tower after adhesion, obtaining partial size is 30 mesh~50 mesh collagen microparticle.
2. the preparation method of collagen microparticle according to claim 1, which is characterized in that in the step S101
In, the collagen solution mass concentration is 28%~32%.
3. the preparation method of collagen microparticle according to claim 1, which is characterized in that in the step S102
In, the temperature of the hot wind is 28 DEG C~40 DEG C.
4. the preparation method of collagen microparticle according to claim 1, which is characterized in that in the step S102
In, the wind speed of the hot wind of tower bottom is 28km/h~38km/h.
5. the preparation method of collagen microparticle according to claim 1, which is characterized in that in the step S102
In, after the step S102 continues 5s~10s, then carry out step S103.
6. the collagen microparticle that the described in any item preparation methods of claim 1-5 obtain.
7. method described in any one of claims 1-6 is preparing the application in collagen product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811057546.5A CN109045362A (en) | 2018-09-11 | 2018-09-11 | Collagen microparticle and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811057546.5A CN109045362A (en) | 2018-09-11 | 2018-09-11 | Collagen microparticle and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109045362A true CN109045362A (en) | 2018-12-21 |
Family
ID=64760101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811057546.5A Pending CN109045362A (en) | 2018-09-11 | 2018-09-11 | Collagen microparticle and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109045362A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112143243A (en) * | 2019-06-28 | 2020-12-29 | 柏登生医股份有限公司 | Scale-derived articles of daily use and method for making same |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6482433B1 (en) * | 1999-06-30 | 2002-11-19 | Givaudan Sa | Encapsulation of active ingredients |
| CN201255558Y (en) * | 2008-08-05 | 2009-06-10 | 蒋中昕 | Spray drying apparatus |
| CN103740791A (en) * | 2013-03-01 | 2014-04-23 | 青岛蒂芬妮生物工程有限公司 | Method for extracting collagen powder from deep sea cod skin |
| CN203999580U (en) * | 2014-07-17 | 2014-12-10 | 王书敏 | Utilize fresh ox bone to prepare the production line of small-molecular peptides |
| CN105188740A (en) * | 2013-03-07 | 2015-12-23 | 普罗菲布里克有限公司 | Powder formulation comprising thrombin and fibrinogen |
| CN105746843A (en) * | 2016-03-18 | 2016-07-13 | 安勇 | Processing method of collagen |
-
2018
- 2018-09-11 CN CN201811057546.5A patent/CN109045362A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6482433B1 (en) * | 1999-06-30 | 2002-11-19 | Givaudan Sa | Encapsulation of active ingredients |
| CN201255558Y (en) * | 2008-08-05 | 2009-06-10 | 蒋中昕 | Spray drying apparatus |
| CN103740791A (en) * | 2013-03-01 | 2014-04-23 | 青岛蒂芬妮生物工程有限公司 | Method for extracting collagen powder from deep sea cod skin |
| CN105188740A (en) * | 2013-03-07 | 2015-12-23 | 普罗菲布里克有限公司 | Powder formulation comprising thrombin and fibrinogen |
| CN203999580U (en) * | 2014-07-17 | 2014-12-10 | 王书敏 | Utilize fresh ox bone to prepare the production line of small-molecular peptides |
| CN105746843A (en) * | 2016-03-18 | 2016-07-13 | 安勇 | Processing method of collagen |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112143243A (en) * | 2019-06-28 | 2020-12-29 | 柏登生医股份有限公司 | Scale-derived articles of daily use and method for making same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Steipel et al. | Electrospray for generation of drug delivery and vaccine particles applied in vitro and in vivo | |
| Zhu et al. | Recent progress of polysaccharide‐based hydrogel interfaces for wound healing and tissue engineering | |
| Sharmila et al. | Fabrication and characterization of Spinacia oleracea extract incorporated alginate/carboxymethyl cellulose microporous scaffold for bone tissue engineering | |
| TWI611816B (en) | Water-soluble hyaluronic acid gel and preparation method thereof | |
| Maleki et al. | Nanofiber-based systems intended for diabetes | |
| Chen et al. | Injectable nanofiber microspheres modified with metal phenolic networks for effective osteoarthritis treatment | |
| CN101601986A (en) | A kind of method for making of chitosan-silicon dioxide compound hollow microballoon and application | |
| Kruk et al. | Alginates combined with natural polymers as valuable drug delivery platforms | |
| CN103965310A (en) | Self-assembling method for preparing fibroin microspheres | |
| CN109045362A (en) | Collagen microparticle and its preparation method and application | |
| CN106924196A (en) | A kind of growth factor-loaded slow-release gelatin microspheres and preparation method thereof | |
| CN105194734A (en) | Chitosan-extracellular matrix tissue repairing membrane and preparation method thereof | |
| Niu et al. | Microencapsulation of mechano growth factor E peptide for sustained delivery and bioactivity maintenance | |
| CN107376005A (en) | A kind of biodegradable medical hemostatic paper and preparation method thereof | |
| CN110313618A (en) | A kind of vitamin D2The preparation method of microcapsules | |
| Yadav et al. | Application of natural polysaccharide for delivery of biopharmaceuticals. | |
| CN107427561A (en) | Skin wound therapeutic combination | |
| RU2013125503A (en) | PRESSED, HIGH-VISCOUS POWDER BASED ON POLYSACCHARIDE AND POLYOL | |
| Ding et al. | Dual green hemostatic sponges constructed by collagen fibers disintegrated from Halocynthia roretzi by a shortcut method | |
| CN106377290B (en) | A kind of medicine-carried titanium nail and load medicine titanium nail and preparation method thereof | |
| Miguel et al. | Biomedical applications of biodegradable polymers in wound care | |
| CN101485904A (en) | Injectable calcium sulphate-based sustained-release implantation component containing medicine-carrying particle and application | |
| CN101868298B (en) | Collagen-based microspheres and methods of preparation and use thereof | |
| Sanchez-Gonzalez et al. | Composite hydrogels of pectin and alginate | |
| Alpizar-Reyes et al. | Recent approaches in alginate-based carriers for delivery of therapeutics and biomedicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20201013 Address after: 528300 unit 1306, building 4, SINOSURE Plaza, No. 46, Tianhong Road, Foshan new city, Lecong community residents committee, Lecong Town, Shunde District, Foshan City, Guangdong Province (application for residence) Applicant after: Kangluoxin (Guangdong) Biotechnology Co.,Ltd. Address before: 529300 Guangdong Changsha Jiangmen Street office Changsha 7 Baohua South Road 703 703 Applicant before: Yu Bizhi |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181221 |