CN109006878A - A kind of control method of mango shot hole - Google Patents
A kind of control method of mango shot hole Download PDFInfo
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- CN109006878A CN109006878A CN201810846949.1A CN201810846949A CN109006878A CN 109006878 A CN109006878 A CN 109006878A CN 201810846949 A CN201810846949 A CN 201810846949A CN 109006878 A CN109006878 A CN 109006878A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Microbiology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
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- Organic Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of control methods of mango shot hole, each of which month sprays mango: 20~30 parts of jute streptomycete secretion, 2~5 parts of muscardine pulvis, 0.1~0.5 part of Kocide SD, 0.1~1 part of microelement, 2~5 parts of titanium dioxide, 1~6 part of plant alcohol extract, 2~5 parts of chitosan, 2~6 parts of soybean protein, 1~5 part of calcium hydroxide using the reagent that the raw material of following mass parts forms, and citric acid is used to adjust actual pH value as 5.5~6.Using this method biocontrol of mango shot hole, mango hardly happens mango shot hole disease.
Description
Technical field
The present invention relates to planting fruit trees industry technical fields, and in particular to a kind of control method of mango shot hole.
Background technique
Mango shot hole is mainly caused harm the organs such as the tender branch of mango, blade, flower and fruit.The tender leaf infected mostly from
Lower epidermis starts crineous occur to black, the scab of subcircular, leaf curling deformity.Aggrieved compared with old leaf piece, the back side, which generates, is permitted
The small blackspot of more protrusions, center is split;Elliptical shaped lobes are formed in middle arteries, the stigma that center is split, leaf curling is abnormal when serious
Shape is easy to fall off.Tiny stain and grey mould layer are grown when wet weather on scab, this is the aceravlus of pathogen and mitogenetic
Spore.Branch is aggrieved to there is irregular grey patch.Green fruit is aggrieved, generates many protrusions in the base portion of fruit
, the spot of taupe, about 5 millimeters of diameter, the sick roughening lignifying of portion's pericarp, center is star-shaped to split, moist duration
Pore and the mould layer of grey, disease fruit are easy to fall off out.Mango shot hole is mainly infected by the full bacterium of mango scab circle and is caused.
Summary of the invention
The technical issues of the present invention overcomes in the prior art, mango easily causes shot hole disease, provides a kind of mango sore
The control method of scall.
To solve the above problems, the present invention adopts the following technical scheme that:
A kind of control method of mango shot hole includes the following steps:
A kind of control method of mango shot hole, each of which month spray awns using the reagent that the raw material of following mass parts forms
Fruit: 20~30 parts of jute streptomycete secretion, 2~5 parts of muscardine pulvis, 0.1~0.5 part of Kocide SD, microelement 0.1~
1 part, 2~5 parts of titanium dioxide, 1~6 part of plant alcohol extract, 2~5 parts of chitosan, 2~6 parts of soybean protein, calcium hydroxide 1~5
Part, and citric acid is used to adjust actual pH value as 5.5~6.
Further, the plant alcohol extract is by Buffalo bur alcohol extract, horseshoe skin alcohol extracting thing, citrus bark alcohol
Extract composition.
Further, the microelement is made of the following raw material of mass parts: 1~5 part of inositol, glycine 2~3
Part, 3~6 parts of niacin, vitamin B12~6 part, 1~12 part of vitamin B6,2~7 parts of manganese sulfate, 1~3 part of zinc sulfate, permanganic acid
4~8 parts of sodium, 1~5 part of copper sulphate, 2~5 parts of calcium chloride, 6~9 parts of potassium nitrate, 1~8 part of ammonium nitrate, 3~15 parts of potassium hydrogen phosphate,
5~7 parts of magnesium sulfate.
Further, the Buffalo bur alcohol extract, horseshoe skin alcohol extracting thing, citrus bark alcohol extracting thing are by as follows
Method is extracted to obtain: horseshoe skin, Buffalo bur, citrus bark crushed after being dried being sieved with 100 mesh sieve, by smashed horseshoe
Skin, Buffalo bur, citrus bark are placed in supercritical extract axe, are 29 DEG C in extraction temperature using 20% ethyl alcohol as entrainer,
Extracting pressure is 40MPa, and the flow velocity of carbon dioxide is that the isolated plant alcohol extracting thing after 20min is extracted under 35L/min.
Further, the jute streptomycete secretion is prepared by following technique, and training will be placed in jute streptomycete
It supports in base after 37 DEG C of 5~7d of culture, filtering removes bacterial strain, then using mass ratio for the ethyl alcohol of 1:5, ethylene glycol is extractant
It extracts to obtain the jute streptomycete secretion;
The formula of the culture medium are as follows: bagasse 10g;(NH4)2S044g;KH2P042g;CaCl20.3g;MgS04
0.4g;Peptone 2g;Carbon source 1Og;FeS04·7H20 8.5mg;MnS04·H20 1.5mg;ZnS04·7H20 4.2mg;
CoCl2·6H20 2.5mg;pH5.5±0.5.
The full bacterium inoculation of mango scab circle is trained with containing in the jute streptomycete cultivation of cervical secretions base that above-mentioned preparation method obtains
It supports, the full bacterium of mango scab circle does not survive.
The present invention has the advantages that compared with prior art
In the present invention jute streptomycete secretion by after the culture medium culture containing bagasse again using ethyl alcohol, ethylene glycol into
Organic acid, alcohol, ketone, terpene isoreactivity ingredient is obtained by extraction in row, and it can be killed with muscardine, Kocide SD, the cooperation of plant alcohol extract
The full bacterium pathogen of mango scab circle and its conidium, microelement supplement the nutrients to mango, improve infecting for mango anti-microbial pathogen
Disease resistance.Titanium dioxide, chitosan, soybean protein, calcium hydroxide can form microemulsion for jute streptomycete secretion, white deadlock
Bacterium, Kocide SD and plant alcohol extract are wrapped in microemulsion, can form protective layer on mango surface when sprinkling, effective pre-
Shot hole occurs for anti-mango.
Specific embodiment
Below with reference to embodiment and test, the invention will be further described.
Embodiment 1
A kind of control method of mango shot hole, each of which month spray awns using the reagent that the raw material of following mass parts forms
Fruit: 30 parts of jute streptomycete secretion, 2 parts of muscardine pulvis, 0.5 part of Kocide SD, 0.1 part of microelement, titanium dioxide 5
Part, 1 part of plant alcohol extract, 5 parts of chitosan, 2 parts of soybean protein, 5 parts of calcium hydroxide, and actual pH value is adjusted using citric acid
It is 5.5.The microelement is made of the following raw material of mass parts: 5 parts of inositol, 2 parts of glycine, 6 parts of niacin, vitamin
B12 parts, 12 parts of vitamin B6,2 parts of manganese sulfate, 3 parts of zinc sulfate, 4 parts of sodium permanganate, 5 parts of copper sulphate, 2 parts of calcium chloride, nitric acid
9 parts of potassium, 1 part of ammonium nitrate, potassium hydrogen phosphate~15 part, 5 parts of magnesium sulfate.The plant alcohol extract by Buffalo bur alcohol extract,
Horseshoe skin alcohol extracting thing, citrus bark alcohol extracting thing composition, the plant alcohol extract extracts by the following method to be obtained: by horseshoe
Skin, Buffalo bur, citrus bark crushed after being dried sieve with 100 mesh sieve, by smashed horseshoe skin, Buffalo bur, mandarin tree
Skin is placed in supercritical extract axe, is 29 DEG C, extracting pressure 40MPa in extraction temperature using 20% ethyl alcohol as entrainer, dioxy
The flow velocity for changing carbon is that the isolated plant alcohol extracting thing after 20min is extracted under 35L/min.
The jute streptomycete secretion is prepared by following technique, will be placed in culture medium in jute streptomycete in 37
After DEG C culture 5d, filtering removal bacterial strain, then using mass ratio for the ethyl alcohol of 1:5, ethylene glycol is that extractant extracts to obtain
The jute streptomycete secretion;The formula of the culture medium are as follows: bagasse 10g;(NH4)2S044g;KH2P042g;
CaCl20.3g;MgS040.4g;Peptone 2g;Carbon source 1Og;FeS04·7H20 8.5mg;MnS04·H20 1.5mg;
ZnS04·7H20 4.2mg;CoCl2·6H20 2.5mg;pH5.5±0.5.
Embodiment 2
A kind of control method of mango shot hole, each of which month spray awns using the reagent that the raw material of following mass parts forms
Fruit: 20 parts of jute streptomycete secretion, 5 parts of muscardine pulvis, 0.1 part of Kocide SD, 1 part of microelement, 2 parts of titanium dioxide,
6 parts of plant alcohol extract, 2 parts of chitosan, 6 parts of soybean protein, 1 part of calcium hydroxide, and use citric acid adjust actual pH value for
6.The microelement is made of the following raw material of mass parts: 1 part of inositol, 3 parts of glycine, 3 parts of niacin, 6 parts of vitamin B1,
1 part of vitamin B6,7 parts of manganese sulfate, 1 part of zinc sulfate, 8 parts of sodium permanganate, 1 part of copper sulphate, 5 parts of calcium chloride, 6 parts of potassium nitrate, nitre
8 parts of sour ammonium, 3 parts of potassium hydrogen phosphate, 7 parts of magnesium sulfate.The plant alcohol extract is by Buffalo bur alcohol extract, horseshoe skin alcohol extracting
Object, citrus bark alcohol extracting thing composition, the plant alcohol extract extracts by the following method to be obtained: by horseshoe skin, Buffalo bur,
Citrus bark crushed after being dried sieves with 100 mesh sieve, and smashed horseshoe skin, Buffalo bur, citrus bark are placed in overcritical extraction
It takes in axe, is 29 DEG C in extraction temperature, the flow velocity of extracting pressure 40MPa, carbon dioxide are using 20% ethyl alcohol as entrainer
The isolated plant alcohol extracting thing after extraction 20min under 35L/min.
The jute streptomycete secretion is prepared by following technique, will be placed in culture medium in jute streptomycete in 37
After DEG C culture 7d, filtering removal bacterial strain, then using mass ratio for the ethyl alcohol of 1:5, ethylene glycol is that extractant extracts to obtain
The jute streptomycete secretion;The formula of the culture medium are as follows: bagasse 10g;(NH4)2S044g;KH2P042g;
CaCl20.3g;MgS040.4g;Peptone 2g;Carbon source 1Og;FeS04·7H20 8.5mg;MnS04·H20 1.5mg;
ZnS04·7H20 4.2mg;CoCl2·6H20 2.5mg;pH5.5±0.5.
Embodiment 3
A kind of control method of mango shot hole, each of which month spray awns using the reagent that the raw material of following mass parts forms
Fruit: 25 parts of jute streptomycete secretion, 3 parts of muscardine pulvis, 0.3 part of Kocide SD, 0.3 part of microelement, titanium dioxide 3
Part, 5 parts of plant alcohol extract, 5 parts of chitosan, 4 parts of soybean protein, 3 parts of calcium hydroxide, and actual pH value is adjusted using citric acid
It is 5.8.The microelement is made of the following raw material of mass parts: 3 parts of inositol, 2 parts of glycine, 4 parts of niacin, vitamin
B15 parts, 10 parts of vitamin B6,5 parts of manganese sulfate, 2 parts of zinc sulfate, 5 parts of sodium permanganate, 3 parts of copper sulphate, 3 parts of calcium chloride, potassium nitrate
8 parts, 7 parts of ammonium nitrate, 12 parts of potassium hydrogen phosphate, 8 parts of magnesium sulfate.The plant alcohol extract is by Buffalo bur alcohol extract, horseshoe
Skin alcohol extracting thing, citrus bark alcohol extracting thing composition, the plant alcohol extract extracts by the following method to be obtained: by horseshoe skin, Huang
Flowers and plants wooden slippers, citrus bark crushed after being dried sieve with 100 mesh sieve, and smashed horseshoe skin, Buffalo bur, citrus bark are placed in
It is 29 DEG C, extracting pressure 40MPa in extraction temperature using 20% ethyl alcohol as entrainer in supercritical extract axe, carbon dioxide
Flow velocity is that the isolated plant alcohol extracting thing after 20min is extracted under 35L/min.
The jute streptomycete secretion is prepared by following technique, will be placed in culture medium in jute streptomycete in 37
After DEG C culture 6d, filtering removal bacterial strain, then using mass ratio for the ethyl alcohol of 1:5, ethylene glycol is that extractant extracts to obtain
The jute streptomycete secretion;The formula of the culture medium are as follows: bagasse 10g;(NH4)2S044g;KH2P042g;
CaCl20.3g;MgS040.4g;Peptone 2g;Carbon source 1Og;FeS04·7H20 8.5mg;MnS04·H20 1.5mg;
ZnS04·7H20 4.2mg;CoCl2·6H20 2.5mg;pH5.5±0.5.
Comparative example 1
Comparative example 1 and the control method of the mango shot hole of embodiment 1 are essentially identical, and difference is, comparative example 1 does not contain
Jute streptomycete secretion.
Comparative example 2
Comparative example 2 and the control method of the mango shot hole of embodiment 1 are essentially identical, and difference is, comparative example 2 does not contain
Muscardine pulvis.
Comparative example 3
Comparative example 3 and the control method of the mango shot hole of embodiment 1 are essentially identical, and difference is, comparative example 3 does not contain
Titanium dioxide, chitosan, soybean protein and calcium hydroxide.
Comparative example 4
Comparative example 4 and the control method of the mango shot hole of embodiment 1 are essentially identical, and difference is, comparative example 4 does not contain
Plant alcohol extract.
Comparative example 5
Comparative example 5 is using Bordeaux mixture and sprinkling mango can be mixed like property pulvis, and every month, sprinkling was primary.
After the mango shot hole control method biocontrol of mango of 1~embodiment of embodiment 3 and 1~comparative example of comparative example 5, awns
The diseases prevention rate of fruit is recorded in the following table 1.
Table 1
Detection | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 |
Disease incidence % | 0 | 0 | 0 | 75% | 12% | 37% | 43% | 3% |
By in table 1 it is found that 1~embodiment of embodiment, 3 mango does not generate shot hole disease, and 1~comparative example of comparative example 5
There is different degrees of mango shot hole disease.Illustrate the present invention using jute streptomycete secretion, muscardine pulvis, hydroxide
Copper, microelement, titanium dioxide, plant alcohol extract, chitosan, soybean protein, calcium hydroxide mutual cooperation can be killed preferably
The full bacterium of mango scab circle, effectively preventing mango shot hole.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (5)
1. a kind of control method of mango shot hole, which is characterized in that each of which month is formed using the raw material of following mass parts
Reagent sprays mango: 20 ~ 30 parts of jute streptomycete secretion, 2 ~ 5 parts of muscardine pulvis, 0.1 ~ 0.5 part of Kocide SD, micro member
Plain 0.1 ~ 1 part, 2 ~ 5 parts of titanium dioxide, 1 ~ 6 part of plant alcohol extract, 2 ~ 5 parts of chitosan, 2 ~ 6 parts of soybean protein, calcium hydroxide 1 ~ 5
Part, and citric acid is used to adjust actual pH value as 5.5 ~ 6.
2. a kind of control method of mango shot hole according to claim 1, which is characterized in that the plant alcohol extract
It is made of Buffalo bur alcohol extract, horseshoe skin alcohol extracting thing, citrus bark alcohol extracting thing.
3. a kind of control method of mango shot hole according to claim 1, which is characterized in that the microelement by
The following raw materials of mass parts forms: 1 ~ 5 part of inositol, 2 ~ 3 parts of glycine, 3 ~ 6 parts of niacin, vitamin B12 ~ 6 part, vitamin B6 1 ~
12 parts, 2 ~ 7 parts of manganese sulfate, 1 ~ 3 part of zinc sulfate, 4 ~ 8 parts of sodium permanganate, 1 ~ 5 part of copper sulphate, 2 ~ 5 parts of calcium chloride, potassium nitrate 6 ~ 9
Part, 1 ~ 8 part of ammonium nitrate, 3 ~ 15 parts of potassium hydrogen phosphate, 5 ~ 7 parts of magnesium sulfate.
4. a kind of control method of mango shot hole according to claim 2, which is characterized in that the Buffalo bur
Alcohol extract, horseshoe skin alcohol extracting thing, citrus bark alcohol extracting thing are extracted obtain by the following method: by horseshoe skin, Buffalo bur,
Citrus bark crushed after being dried sieves with 100 mesh sieve, and smashed horseshoe skin, Buffalo bur, citrus bark are placed in overcritical extraction
It takes in axe, is 29 DEG C in extraction temperature, the flow velocity of extracting pressure 40MPa, carbon dioxide are using 20% ethyl alcohol as entrainer
The isolated plant alcohol extracting thing after extraction 20min under 35L/min.
5. a kind of control method of mango shot hole according to claim 1, which is characterized in that the jute streptomycete point
Secretion is prepared by following technique, will be placed in culture medium in jute streptomycete after 37 DEG C of 5 ~ 7d of culture, be filtered off degerming
Strain, then using mass ratio for the ethyl alcohol of 1:5, ethylene glycol is that extractant extracts to obtain the jute streptomycete secretion;
The formula of the culture medium are as follows: bagasse 10g;(NH4)2S044g;KH2P042g;CaCl2 0.3g;MgS04 0.4g;
Peptone 2g;Carbon source 1Og;FeS04·7H20 8.5mg;MnS04·H20 1.5mg;ZnS04·7H20 4.2 mg;
CoCl2·6H20 2.5mg;PH5.5 ± 0.5.
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Citations (2)
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2018
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CN108029709A (en) * | 2017-10-16 | 2018-05-15 | 钟山佳欣果蔬有限责任公司 | A kind of fungicide for preventing citrus scab and preparation method thereof |
CN107750694A (en) * | 2017-11-23 | 2018-03-06 | 荔浦县万家兴果蔬专业合作社 | A kind of high-yield method of Ponkan |
Non-Patent Citations (4)
Title |
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何胜强,郭青: "《芒果疮痂病及其防治》", 《植物医生》 * |
刘步林: "《农药剂型加工技术 第二版》", 31 October 1998, 化学工业出版社 * |
李少华: "《植物农药》", 31 October 2012, 中国农业科学技术出版社 * |
杨敬辉,文平兰,陈宏州,等: "《放线菌NF0919的鉴定及生防活性》", 《西北农业学报》 * |
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