CN108956432A - A kind of streaming high speed super-resolution imaging device and method based on structure light - Google Patents
A kind of streaming high speed super-resolution imaging device and method based on structure light Download PDFInfo
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Abstract
The invention proposes a kind of streaming high speed super-resolution imaging device and method based on structure light.Apparatus of the present invention include: femtosecond pulse laser, coupler, the first photodetector, arbitrary waveform generator, the first pump laser, the first wavelength division multiplexer, dispersion compensating fiber, the second wavelength division multiplexer, the second pump laser, electrooptic modulator, circulator, collimator, diffraction grating, the first plano-convex lens, the second plano-convex lens, microcobjective, observation object, the second photodetector, high-speed oscilloscope, data processing unit.The present invention carries out coding by spectrum of the device to femtosecond pulse and generates the pulse with a variety of phases, optical spectrum encoded pulse is focused on observation object by the structure light that diffraction obtains linear one dimensional dispersion pulse, the information coding of observation object, by photodetector and high-speed oscilloscope continuous probe and acquisition, and obtains high quality graphic into linear one dimensional dispersion pulse in data processing unit.
Description
Technical field
The invention belongs to high speed super-resolution imaging field more particularly to a kind of streaming high speed super-resolution based on structure light at
As device and method.
Background technique
Imaging technique is human knowledge one of naturally with the important tool of nature remodeling, at present in numerous fields
It has been widely used.For the screening and classification in microfluidic channel inner cell or particle etc., there is high-space resolution
It can make the cell number not only screened and classified with the device and method of high time resolution ability and energy long-time continuous imaging
Amount and speed are greatly improved, and are able to achieve very high screening and nicety of grading.Research has high spatial and high time point
The imaging device and method distinguished are always one of the target that people pursue, but current imaging technique discuss respectively mostly how
Single raising spatial resolution or temporal resolution, fail simultaneously to improve the two, and existing in imaging process
High speed super-resolution device and method cannot achieve long-time continuous observation.
Currently, most widely used imaging technique is using charge coupling device imaging sensor and Complimentary Metal-Oxide
Semiconductor imaging device, but it cannot achieve the continuous probe of ultrafast process due to imaging frame rate is low.Pumping-spy
The temporal resolution of ps or even fs magnitude may be implemented in survey technology, but needs that measurement is repeated several times, and is usually applicable only to non-broken
The observation of bad property, artificial event, and its limitation that can not break through diffraction limit obtains the image of high-space resolution.
The current main method for improving spatial resolution has saturated structures to illuminate microtechnic, the micro- skill of stimulated emission depletion
Art, photoactivation positioning microtechnic and random optical reconstruct microtechnic etc., these imaging skills with high-space resolution ability
Its temporal resolution of art can only achieve microsecond rank.
It therefore, currently can be with there is an urgent need to a kind of imaging device with high spatial and high time resolution performance and method
Realize in microfluidic channel cell or particle etc. is continuous observes for a long time.
Summary of the invention
For existing imaging technique, only single aspect is improved in temporal resolution and spatial resolution mostly, is failed
The defect that the two is improved simultaneously, it is a kind of based on structure to proposing when microfluidic channel inner cell or particle etc. are imaged
The streaming high speed super-resolution imaging device and method of light, while there is the performance of high spatial and high time resolution, and can overcome
Existing high speed super-resolution is not able to satisfy the requirement of long-time continuous observation, to realize to microfluidic channel inner cell or particle etc.
Continuous observation obtains the image of high quality, meets the speed and precision requirement of screening, classification.
The technical solution of apparatus of the present invention is a kind of streaming high speed super-resolution imaging device based on structure light, and feature exists
In, comprising: femtosecond pulse laser, coupler, the first photodetector, arbitrary waveform generator, the first pump laser,
One wavelength division multiplexer, dispersion compensating fiber, the second wavelength division multiplexer, the second pump laser, electrooptic modulator, circulator, standard
Straight device, diffraction grating, the first plano-convex lens, the second plano-convex lens, microcobjective, observation object, the second photodetector, high speed
Oscillograph, data processing unit；
The femtosecond pulse laser is connect with the coupler；The coupler, the first photodetector, any wave
Shape generator is sequentially connected in series；The coupler, the first wavelength division multiplexer, dispersion compensating fiber, the second wavelength division multiplexer
It is sequentially connected in series；First wavelength division multiplexer is connect with first pump laser；Second wavelength division multiplexer with
The second pump laser connection；The arbitrary waveform generator is connect with the electrooptic modulator；Second wavelength-division is multiple
It is connect with device with the electrooptic modulator；The electrooptic modulator, circulator, collimator are sequentially connected in series；The diffraction
Grating d at a certain distance1And angle, θ1It is placed in front of the collimator；First plano-convex lens d at a certain distance2The angle and
Spend θ2It is placed in front of the diffraction grating；Second plano-convex lens d at a certain distance3Parallel is placed in first plano-convex
In front of lens；Microcobjective d at a certain distance4Parallel is placed in front of second plano-convex lens；The observation pair
As d at a certain distance5It is placed in front of the microcobjective in parallel；The circulator, the second photodetector, high speed oscillography
Device, data processing unit are sequentially connected in series.
The femtosecond pulse laser is for generating femtosecond pulse；The coupler is used to femtosecond pulse being divided into the first via
Optical signal pulses and the second tunnel optical signal pulses；First photodetector is for being converted to first via optical signal pulses
Pulse electrical signal；The arbitrary waveform generator generates N kind phase sinusoidal wave, N >=3 according to pulse electrical signal is synchronous；Pass through institute
It states dispersion compensating fiber and time domain stretching is carried out to the second tunnel optical signal pulses；Pass through the first wavelength division multiplexer, the first pumping laser
Device, the second wavelength division multiplexer and the second pump laser realize distributed raman amplification to compensate in the dispersion compensating fiber
Optical power loss of the second tunnel optical signal pulses in time domain stretching；What the electrooptic modulator was stretched and was compensated to time domain pull-up
Second tunnel optical signal pulses carry out light pulse modulation according to N kind phase sinusoidal wave and generate optical spectrum encoded pulse；The circulator
And the collimator is for adjusting optical spectrum encoded impulse ejection angle；Light after adjusting launch angle on the diffraction grating
The structure light of the linear one-dimensional dispersion pulse of the pulse scatter open form of spectral encoding；First plano-convex lens, second plano-convex
Lens and the microcobjective lead in linear one dimensional dispersion pulse concentration to the observation object being located in microfluidic channel
Cross the one-dimensional dispersion pulse of observation object reflecting linear；The microcobjective, second plano-convex lens, first plano-convex are saturating
The linear one dimensional dispersion pulse of reflection is reduced to pulse by mirror, the diffraction grating；Pulse is coupled by the collimator
Into the circulator；Pulse is converted to analog electrical signal by second photodetector；The high-speed oscilloscope acquires mould
Quasi- electric signal is converted to digital electric signal, and digital electric signal is transmitted to the data processing unit；The data processing list
Member processing digital electric signal obtains image and is stored.
The technical solution of the method for the present invention is a kind of streaming high speed super-resolution imaging method based on structure light, specific method
Are as follows:
Step 1: femtosecond pulse laser generates femtosecond pulse；
Step 2: femtosecond pulse is divided into first via optical signal pulses and the second tunnel optical signal pulses by coupler；First light
First via optical signal pulses are converted to pulse electrical signal by electric explorer；Arbitrary waveform generator is produced according to pulse electrical signal is synchronous
Raw N kind phase sinusoidal wave, N >=3；Time domain stretching is carried out to the second tunnel optical signal pulses by dispersion compensating fiber；Pass through first
Wavelength division multiplexer, the first pump laser, the second wavelength division multiplexer and the second pump laser are realized in dispersion compensating fiber
Distributed raman amplification is to compensate optical power loss of the second tunnel optical signal pulses in time domain stretching；Electrooptic modulator is to time domain
Upper stretching and the second tunnel optical signal pulses compensated generate optical spectrum encoded according to the progress light pulse modulation of N kind phase sinusoidal wave
Pulse；
Step 3: circulator and collimator are for adjusting optical spectrum encoded impulse ejection angle；It will transmitting on diffraction grating
The structure light of the optical spectrum encoded linear one-dimensional dispersion pulse of pulse scatter open form after angle adjustment；First plano-convex lens, second
Plano-convex lens and microcobjective work as sight in linear one dimensional dispersion pulse concentration to the observation object being located in microfluidic channel
Object is surveyed when high-speed mobile, the different location of observation object is illuminated in linear one dimensional dispersion pulse, by the table of observation object
Face information coding spectrally, completes space encoding to linear one dimensional dispersion pulse, the line that then will be reflected from observation object
Property one-dimensional dispersion pulse be reduced to carry observation pair by microcobjective, the second plano-convex lens, the first plano-convex lens, diffraction grating
As the pulse of surface information；The pulse for carrying observation object surface information is coupled into circulator by collimator；
Step 4: the pulse for carrying observation object surface information is converted to analog electrical signal by the second photodetector；
Step 5: high-speed oscilloscope acquisition analog electrical signal is converted to digital electric signal, and digital electric signal is transmitted to number
According to processing unit；
Step 6: data processing unit processing digital electric signal obtains image and is stored.
The present invention has the advantages that the optical instrument in imaging device is common optical instrument, realized convenient for system；
The bottleneck of diffraction limit can be broken through, realizes high spatial resolution；The bottleneck of energy break through resolution capability, realizes frame number
The imaging frame speed of 10MHz or more；High-space resolution module will be realized in imaging technique and realize that high time resolution module integrates
Together, the imaging device can be made while there is high spatial resolution and high time resolution；It is able to achieve to microfluidic channel
The continuous of inner cell or particle etc. is observed for a long time.
Detailed description of the invention
Fig. 1: the structural schematic diagram of the invention patent；
Fig. 2: there are three types of the sine waves of out of phase for the preset tool that arbitrary waveform generator generates；
Fig. 3: by the schematic diagram for the optical spectrum encoded pulse that electrooptic modulator generates.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
A kind of streaming high speed super-resolution imaging device based on structure light is as shown in Figure 1 characterized by comprising femtosecond
Pulse laser 101, coupler 102, the first photodetector 103, arbitrary waveform generator 104, the first pump laser
105, the first wavelength division multiplexer 106, dispersion compensating fiber 107, the second wavelength division multiplexer 108, the second pump laser 109, light
Electric modulator 110, circulator 111, collimator 112, diffraction grating 113, the first plano-convex lens 114, the second plano-convex lens 115,
Microcobjective 116, observation object 117, the second photodetector 118, high-speed oscilloscope 119, data processing unit 120；
101 type selecting of femtosecond pulse laser is the ti sapphire laser Vitara-T-HP of center wavelength 800nm；Institute
State the TW805R2F1 that 102 type selecting of coupler is Thorlabs；First photodetector, 103 type selecting is Newport-1481-
s；104 type selecting of arbitrary waveform generator is the M8195A that the U.S. is Deco skill；First pump laser, 105 type selecting is
Central wavelength is the pump laser of 633nm；First wavelength division multiplexer, 106 type selecting is the NR73F1 of Thorlabs；It is described
107 type selecting of dispersion compensating fiber is the dispersion compensating fiber that group velocity dispersion is -800ps/nm；Second wavelength division multiplexer
108 type selectings are the NR71F1 of Thorlabs；Second pump laser, 109 type selecting is that the pumping that center wavelength is 532nm swashs
Light device；110 type selecting of electrooptic modulator is the Mach-Zehnder modulators of 800nm wave band；111 type selecting of circulator is
The circulator of 800nm wave band；112 type selecting of collimator is the C40FC-B of Thorlabs；113 type selecting of diffraction grating is
Incisure density 1200/nm；First plano-convex lens, 114 type selecting is focal length f=100nm；Second plano-convex lens, 115 type selecting
For focal length f=50nm；116 type selecting of microcobjective is numerical aperture 0.65, enlargement ratio 40x；The observation object 117 selects
Type is cell or microparticle；Second photodetector, 118 type selecting is Newport-1481-s；The high-speed oscilloscope
119 type selectings are the DSA91304A that the U.S. is Deco skill；120 type selecting of data processing unit is server.
The femtosecond pulse laser 101 is connect with the coupler 102；The coupler 102, the first photodetection
Device 103, arbitrary waveform generator 104 are sequentially connected in series；The coupler 102, the first wavelength division multiplexer 106, dispersion are mended
Repay optical fiber 107, the second wavelength division multiplexer 108 is sequentially connected in series；First wavelength division multiplexer 106 swashs with first pumping
Light device 105 connects；Second wavelength division multiplexer 108 is connect with second pump laser 109；The random waveform occurs
Device 104 is connect with the electrooptic modulator 110；Second wavelength division multiplexer 108 is connect with the electrooptic modulator 110；Institute
The electrooptic modulator 110 stated, circulator 111, collimator 112 are sequentially connected in series；The diffraction grating 113 d at a certain distance1
=40mm and angle, θ1=60 ° are placed in 112 front of collimator；First plano-convex lens 114 d at a certain distance2=
100mm and angle, θ2=4 ° are placed in 113 front of diffraction grating；Second plano-convex lens 115 d at a certain distance3=
150mm is parallel to be placed in front of first plano-convex lens 114；The microcobjective 116 d at a certain distance4=50mm is flat
Capable is placed in 115 front of the second plano-convex lens；The observation object 117 d at a certain distance5=8mm is placed in institute in parallel
State 116 front of microcobjective；The circulator 111, the second photodetector 118, high-speed oscilloscope 119, data processing list
Member 120 is sequentially connected in series.
The femtosecond pulse laser 101 is for generating femtosecond pulse；The coupler 102 is for femtosecond pulse to be divided into
First via optical signal pulses and the second tunnel optical signal pulses；First photodetector 103 is used for first via optical signal
Pulses switch is pulse electrical signal；The arbitrary waveform generator 104 generates N kind phase sinusoidal according to pulse electrical signal is synchronous
Wave, N >=3；Time domain stretching is carried out to the second tunnel optical signal pulses by the dispersion compensating fiber 107；It is multiple by the first wavelength-division
With device 106, the first pump laser 105, the second wavelength division multiplexer 108 and the second pump laser 109 in the dispersion compensation
Distributed raman amplification is realized in optical fiber 107 to compensate optical power loss of the second tunnel optical signal pulses in time domain stretching；Institute
It states the second tunnel optical signal pulses that electrooptic modulator 110 is stretched and compensated to time domain pull-up and light is carried out according to N kind phase sinusoidal wave
Impulse modulation generates optical spectrum encoded pulse；The circulator 111 and the collimator 112 are for adjusting optical spectrum encoded arteries and veins
Rush launch angle；Optical spectrum encoded pulse scatter open form is linear one-dimensional after adjusting launch angle on the diffraction grating 113
The structure light of dispersion pulse；First plano-convex lens 114, second plano-convex lens 115 and the microcobjective 116 will
In linear one dimensional dispersion pulse concentration to the observation object 117 being located in microfluidic channel, and pass through 117 reflected ray of observation object
The one-dimensional dispersion pulse of property；The microcobjective 116, first plano-convex lens 114, described spreads out at second plano-convex lens 115
It penetrates grating 113 and the linear one dimensional dispersion pulse of reflection is reduced to pulse；Pulse is coupled into institute by the collimator 112
State circulator 111；Pulse is converted to analog electrical signal by second photodetector 118；The high-speed oscilloscope 119 is adopted
Collection analog electrical signal is converted to digital electric signal, and digital electric signal is transmitted to the data processing unit 120；The data
Processing unit 120 handles digital electric signal and obtains image and stored.
Below with reference to Fig. 1 to Fig. 3, embodiments of the present invention are introduced.The specific embodiment of the invention realizes that steps are as follows:
Step 1: femtosecond pulse laser 101 generates femtosecond pulse, and wherein a length of 800nm of cardiac wave, band are wider than 60nm, weight
Complex frequency is 10MHz, and the frame rate of imaging is equal to the repetition rate of pulse, realizes the image taking speed of 10MHz；
Step 2: by coupling ratio be 90:10 coupler 102 by femtosecond pulse be divided into first via optical signal pulses and
Second tunnel optical signal pulses；First via optical signal pulses are converted to pulse electrical signal by the first photodetector 103；Random waveform
Generator 104 generates N=3 kind phase sinusoidal wave according to pulse electrical signal is synchronous, and phase is respectively120 °, 240 °, three kinds
The sinusoidal wave duration of out of phase is equal to the pulse period of femtosecond pulse laser, and arbitrary waveform generator is successive
Three kinds of outs of phase of generation sine wave as shown in Fig. 2, by photodetector as trigger guarantee modulation synchronism；
Time domain stretching is carried out to the second tunnel optical signal pulses by dispersion compensating fiber 107；Pass through the first wavelength division multiplexer 106, first
Pump laser 105,108 second pump laser 109 of the second wavelength division multiplexer realize distribution in dispersion compensating fiber 107
Raman amplifiction is to compensate optical power loss of the second tunnel optical signal pulses in time domain stretching；Electrooptic modulator 110 is in time domain
It stretches and the second tunnel optical signal pulses compensated is optical spectrum encoded according to the progress light pulse modulation generation of N=3 kind phase sinusoidal wave
Pulse, as shown in Figure 3, wherein red, orange, yellow etc. only to represent in pulse different wave length after time dispersal unit in time
It separates, does not represent actual wavelength.
Step 3: circulator 111 and collimator 112 are for adjusting optical spectrum encoded impulse ejection angle；Diffraction grating
The structure light of the linear one-dimensional dispersion pulse of optical spectrum encoded pulse scatter open form after adjusting launch angle on 113；First is flat
Convex lens 114, the second plano-convex lens 115 and microcobjective 116 lead to linear one dimensional dispersion pulse concentration to positioned at microfluid
In road as on the observation object 117 of cell or particle, when observation object is when high-speed mobile, linear one dimensional dispersion pulse
The surface information of observation object is encoded to linear one dimensional dispersion pulse spectrally by the different location for illuminating observation object, complete
It is then that the linear one dimensional dispersion pulse reflected from observation object 117 is flat by microcobjective 116, second at space encoding
Convex lens 115, the first plano-convex lens 114, diffraction grating 113 are reduced to carry the pulse of observation object surface information；It carries
The pulse of observation object surface information is coupled into circulator 111 by collimator 112；
Step 4: the pulse for carrying observation object surface information is converted to analog telecommunications by the second photodetector 118
Number；
Step 5: high-speed oscilloscope 119 acquires analog electrical signal and is converted to digital electric signal, and digital electric signal is transmitted
To data processing unit 120；
Step 6: data processing unit 120 handles digital electric signal and obtains image and stored.
To sum up, the optical component in above-mentioned imaging device and method is all common component, is easy to implement, while will be high
Spatial resolution and high time resolution module integrate, and can break through diffraction in linear one-dimensional dispersion pulse transverse direction
The limitation of the limit, improves the spatial resolution of imaging, and realizes the continuous imaging rate of 10MHz frame speed, can be to microfluid
The continuous of channel inner cell or particle etc. is observed for a long time, obtains the image of high quality.
Although femtosecond pulse laser 101 is used more herein, coupler 102, the first photodetector 103, appoints
Meaning waveform generator 104, the first pump laser 105, the first wavelength division multiplexer 106, dispersion compensating fiber 107, the second wavelength-division
Multiplexer 108, the second pump laser 109, electrooptic modulator 110, circulator 111, collimator 112, diffraction grating 113,
One plano-convex lens 114, the second plano-convex lens 115, microcobjective 116, observation object 117, the second photodetector 118, high speed
The terms such as oscillograph 119, data processing unit 120, but a possibility that be not precluded using other terms.Only using these terms
Merely to more easily describing essence of the invention, being construed as any additional limitation all is and present invention essence
What mind was disagreed.
It should be understood that the above-mentioned description for preferred embodiment is more detailed, can not therefore be considered to this
The limitation of invention patent protection range, those skilled in the art under the inspiration of the present invention, are not departing from power of the present invention
Benefit requires to make replacement or deformation under protected ambit, fall within the scope of protection of the present invention, this hair
It is bright range is claimed to be determined by the appended claims.
Claims (3)
1. a kind of streaming high speed super-resolution imaging device based on structure light, characterized by comprising: femtosecond pulse laser
101, coupler 102, the first photodetector 103, arbitrary waveform generator 104, the first pump laser 105, the first wavelength-division
Multiplexer 106, dispersion compensating fiber 107, the second wavelength division multiplexer 108, the second pump laser 109, electrooptic modulator 110,
Circulator 111, collimator 112, diffraction grating 113, the first plano-convex lens 114, the second plano-convex lens 115, microcobjective 116,
Observation object 117, the second photodetector 118, high-speed oscilloscope 119, data processing unit 120；
The femtosecond pulse laser 101 is connect with the coupler 102；The coupler 102, the first photodetector
103, arbitrary waveform generator 104 is sequentially connected in series；The coupler 102, the first wavelength division multiplexer 106, dispersion compensation
Optical fiber 107, the second wavelength division multiplexer 108 are sequentially connected in series；First wavelength division multiplexer 106 and first pumping laser
Device 105 connects；Second wavelength division multiplexer 108 is connect with second pump laser 109；The arbitrary waveform generator
104 connect with the electrooptic modulator 110；Second wavelength division multiplexer 108 is connect with the electrooptic modulator 110；It is described
Electrooptic modulator 110, circulator 111, collimator 112 be sequentially connected in series；The diffraction grating 113 d at a certain distance1With
Angle, θ1It is placed in 112 front of collimator；First plano-convex lens 114 d at a certain distance2And angle, θ2It is placed in described
113 front of diffraction grating；Second plano-convex lens 115 d at a certain distance3Parallel is placed in first plano-convex lens
114 fronts；The microcobjective 116 d at a certain distance4Parallel is placed in 115 front of the second plano-convex lens；The sight
Survey the d at a certain distance of object 1175It is placed in 116 front of microcobjective in parallel；The circulator 111, the second photoelectricity are visited
Device 118, high-speed oscilloscope 119, data processing unit 120 is surveyed to be sequentially connected in series.
2. the streaming high speed super-resolution imaging device according to claim 1 based on structure light, it is characterised in that: described to fly
Pulse per second (PPS) laser 101 is for generating femtosecond pulse；The coupler 102 is used to femtosecond pulse being divided into first via optical signal arteries and veins
Punching and the second tunnel optical signal pulses；First photodetector 103 is used to first via optical signal pulses being converted to pulse
Electric signal；The arbitrary waveform generator 104 generates N kind phase sinusoidal wave, N >=3 according to pulse electrical signal is synchronous；By described
Dispersion compensating fiber 107 carries out time domain stretching to the second tunnel optical signal pulses；It is pumped by the first wavelength division multiplexer 106, first
Laser 105, the second wavelength division multiplexer 108 and the second pump laser 109 realize distribution in the dispersion compensating fiber 107
Formula Raman amplifiction is to compensate optical power loss of the second tunnel optical signal pulses in time domain stretching；The electrooptic modulator 110 is right
It is stretched in time domain and the second tunnel optical signal pulses compensated carries out light pulse modulation generation spectrum volume according to N kind phase sinusoidal wave
The pulse of code；The circulator 111 and the collimator 112 are for adjusting optical spectrum encoded impulse ejection angle；It is described to spread out
Penetrate the structure light of the linear one-dimensional dispersion pulse of pulse scatter open form optical spectrum encoded after launch angle being adjusted on grating 113；
First plano-convex lens 114, second plano-convex lens 115 and the microcobjective 116 are by linear one dimensional dispersion pulse
It focuses on the observation object 117 being located in microfluidic channel, and passes through the one-dimensional dispersion pulse of 117 reflecting linear of observation object；
The microcobjective 116, second plano-convex lens 115, first plano-convex lens 114, the diffraction grating 113 will reflect
Linear one dimensional dispersion pulse be reduced to pulse；Pulse couples optical fiber by the collimator 112；The circulator 111
The pulse that will be coupled into optical fiber is transferred to the second photodetector 118；Second photodetector 118 converts pulse
For analog electrical signal；The high-speed oscilloscope 119 acquires analog electrical signal and is converted to digital electric signal, and digital electric signal is passed
Transport to the data processing unit 120；The data processing unit 120 handles digital electric signal and obtains image and stored.
3. a kind of streaming high speed super-resolution imaging device using described in claim 1 based on structure light is carried out based on structure light
Streaming high speed super-resolution imaging method, which comprises the following steps:
Step 1: the femtosecond pulse laser 101 generates femtosecond pulse；
Step 2: femtosecond pulse is divided into first via optical signal pulses and the second tunnel optical signal pulses by the coupler 102；Institute
It states the first photodetector 103 and first via optical signal pulses is converted into pulse electrical signal；The arbitrary waveform generator 104
N kind phase sinusoidal wave, N >=3 are generated according to pulse electrical signal is synchronous；By the dispersion compensating fiber 107 to the second road optical signal
Pulse carries out time domain stretching；Pass through the first wavelength division multiplexer 106, the first pump laser 105,108 and of the second wavelength division multiplexer
Second pump laser 109 realizes distributed raman amplification to compensate the second road optical signal in the dispersion compensating fiber 107
Optical power loss of the pulse in time domain stretching；The electrooptic modulator 110 pulls up the second road light stretched and compensated to time domain
Signal pulse carries out light pulse modulation according to N kind phase sinusoidal wave and generates optical spectrum encoded pulse；
Step 3: the circulator 111 and the collimator 112 are for adjusting optical spectrum encoded impulse ejection angle；It is described to spread out
Penetrate the structure light of the linear one-dimensional dispersion pulse of pulse scatter open form optical spectrum encoded after launch angle being adjusted on grating 113；
First plano-convex lens 114, second plano-convex lens 115 and the microcobjective 116 are by linear one dimensional dispersion pulse
It focuses on the observation object 117 being located in microfluidic channel, when observation object is when high-speed mobile, linear one dimensional
The different location of observation object is illuminated in dispersion pulse, and the surface information of observation object is encoded to the light of linear one dimensional dispersion pulse
In spectrum, complete space encoding, then by the linear one dimensional dispersion pulse reflected from observation object 117 by microcobjective 116,
Second plano-convex lens 115, the first plano-convex lens 114, diffraction grating 113 are reduced to carry the simple venation of observation object surface information
Punching；The pulse for carrying observation object surface information is coupled into the circulator 111 by the collimator 112；
Step 4: the pulse for carrying observation object surface information is converted to analog telecommunications by second photodetector 118
Number；
Step 5: the high-speed oscilloscope 119 acquires analog electrical signal and is converted to digital electric signal, and digital electric signal is transmitted
To the data processing unit 120；
Step 6: the data processing unit 120 handles digital electric signal and obtains image and stored.
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