CN1089442A - The microorganism fodder additive Manufacturing approach and use - Google Patents
The microorganism fodder additive Manufacturing approach and use Download PDFInfo
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- CN1089442A CN1089442A CN93110883A CN93110883A CN1089442A CN 1089442 A CN1089442 A CN 1089442A CN 93110883 A CN93110883 A CN 93110883A CN 93110883 A CN93110883 A CN 93110883A CN 1089442 A CN1089442 A CN 1089442A
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- bacillus
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- fodder additive
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Abstract
The invention belongs to the biological chemistry and the microbiology field of chemistry, for utilizing bacillus cereus, Bacillus licheniformis, the method for Bacillus circulans, subtilis, bacillus firmus, bacillus megaterium manufacturing microorganism fodder additive.It is simple to have manufacturing process, and bacterial classification is easy to get, the characteristics that cost is low.Use the microorganism fodder additive and the feed of manufacturing of the present invention to be mixed for livestock and poultry such as piglet, growing and fattening pigs, fish, chicken, duck, ox, sheep, rabbit, have no side effect, noresidue can significantly improve growth of animals or poultry speed, improve efficiency of feed utilization, guarantee food sanitation and human consumer's health simultaneously.
Description
The present invention relates to animal microecology, system utilizes in animal body and filters out useful, normal genus bacillus, make active bacteria formulation through the series of process flow process, be used for the Manufacturing approach and use of fodder additives, belong to the biological chemistry and the microbiology field of chemistry.
The fodder industry of China is an emerging industry, and the core of rejuvenation and development fodder industry is a fodder additives.All adopt Methionin, methionine(Met), multivitamin and mineral additive, medicated premix (as microbiotic and chemicals) and nutritional additive etc. now both at home and abroad in a large number, they prepare production by the nutritional needs of different livestock and poultry, the cost height, and the livestock and poultry absorption rate is low, what have also has side effect, develops immunity to drugs, residual, harm livestock and poultry and people's health.Recently, the report that utilizes microorganism to make fodder additives is arranged also, but all specifically do not disclose bacterial strain, the manufacture method of additive and concrete purposes.
As everyone knows, animal and bird intestines microorganism and feed are in the intravital substantial connection of having digested and assimilated of livestock and poultry, and it is not only relevant with the metabolism of carbohydrate, protein, fat and mineral substance, but also have influence on the synthetic of the interior VITAMIN of body.Can prevent livestock and poultry to catch when useful mushroom is normal in the intestines and the harmful bacterium of antagonism, keep micropopulation normalizing in the intestines, produce lactic acid, hydrogen peroxide and antimicrobial substance; Promote digesting and assimilating of nutritive substance etc.The present invention just is based on and carries out growth and breeding after above-mentioned theory utilizes genus bacillus to enter livestock and poultry alimentary tract with the spore state; participate in the enteral prods metabolism on the one hand; help the digestion and the absorption of feed; the function of stimulating immune system on the other hand; be in equilibrium state between the microorganism in the protection intestines, the result reaches the purpose that improves growth of animals or poultry.
Bacterial strain of the present invention is known genus bacillus.Can obtain by normal channel, concrete genus bacillus goods catalogue, (China Committee for Culture Collection of Microorganisms writes, light industry press publishes to see " Chinese bacterial classification catalogue ".October nineteen eighty-three), in the book every strain microorganism is all indicated source, characteristic, purposes, document, substratum and culture temperature etc.
Genus bacillus and feature that the present invention adopts:
1. bacillus cereus (Bacillus cereus)
Give birth in Gram-positive, shaft-like, the gemma, amphitrichous.Having the growth of beta-hydroxy-butanoic acid salt particle, 7% sodium-chlor in the spore, glucose, sucrose are produced acid, is 0.9 * 2.0u and pectinose and N.F,USP MANNITOL are not produced acid, hemolytic test feminine gender, the nitrate reduction test positive, size.
2. Bacillus licheniformis (Bacillus licheniformis)
Give birth in Gram-positive, shaft-like, the gemma, sporangiocyst does not expand, no beta-hydroxy-butanoic acid salt particle in the spore, 7% sodium-chlor is grown, the V-P reacting positive, the starch hydrolysis positive, size is 0.6-0.7 * 2.8-3.0u
3. Bacillus circulans (Bacillus circulans)
G is with former group, Gram-positive, shaft-like.Give birth in the gemma, sporangiocyst does not expand, no beta-hydroxy-butanoic acid salt particle in the spore, the V-P reaction negative, the whole PH5.5 of V-P liquid, the acid of glucose product is aerogenesis not, pectinose does not produce acid, size is 0.9 * 2.1-3.10u
4. subtilis (Bacillus subtilis)
Give birth in Gram-positive, shaft-like, the gemma or partially in life, sporangiocyst expands but is not remarkable, the growth of 7% sodium-chlor, PH5.7 growth, glucose (+), pectinose (±), wood sugar (±), starch hydrolysis, the nitrate reduction test positive, size are 0.7-0.8 * 2.0-3.0u
5. bacillus firmus (Baillus firmus)
Life or the life of inferior end, sporangiocyst seldom expand in Gram-positive, the gemma.The growth of 7% sodium-chlor, the catalase positive, V-P reaction negative, starch hydrolysis, glucose and N.F,USP MANNITOL produce acid, and size is 0.6-0.9 * 1.2-4.0u
6. bacillus megaterium (Bacillus megaterium)
Give birth in Gram-positive, shaft-like, the gemma or partially in life, sporangiocyst expand not obvious, V-P reaction negative, the catalase positive, starch hydrolysis, glucose produce acid, N.F,USP MANNITOL (±), pectinose and the wood sugar product is sour, nitrate reduction test is positive, size is 1.2-1.5 * 2.0-5.0u
The manufacture method of microorganism fodder additive of the present invention:
1. in the substratum in seeding tank, by a kind in 6 kinds of hundred million active bacillus cereuses of every milliliter of culture medium inoculated 2-3, Bacillus licheniformis, Bacillus circulans, subtilis, bacillus firmus, Bacillus circulans, bacillus megaterium, be preferably under 37 ℃ of conditions at 32-37 ℃, cultivated 12-24 hour.
When in the substratum in the seeding tank every milliliter contain 25-30 more than hundred million during active genus bacillus, produce jar ratio of hundred million active genus bacillus of inoculation of medium 80-100 in per 100 milliliters, changing culture in the seeding tank over to a production jar interior substratum mid-deep strata aeration-agitation cultivated 24-48 hour, culture temperature is 28-32 ℃, stirs the number of times per minute 80-150 time
Production jar substratum is formed and a compound method: at first the tap water that purifies is put into and produced jar, add peptone 2%, extractum carnis 0.5%, glucose 0.3%, edible salt 0.5%, PH7-7.2, stir by aqueous solution weight again, 120 ℃ of temperature autoclavings 40-60 minute, being cooled to 28-32 ℃ again can use.
3. when a production jar endospore production rate reaches 85-95%, be about to nutrient solution and change high speed centrifugation equipment collection thalline over to, the thalline of collecting is a canescence, thalline (also can directly mix nutrient solution in starch in the 1 ratio uniform mixing than 3-6 with starch, and without centrifugal collection thalline operation) through 70-80 ℃ of drying and grinding repeatedly, cross 80 mesh sieves and make the bacterium powder, make the microorganism fodder additive that every gram contains hundred million active genus bacillus of 5-8 by the ratio row of bacterium powder (30%), glucose (10%), starch (60%) then.Also can directly the thalline of producing jar interior nutrient solution or collect through high speed centrifugation equipment be mixed with grape powder, starch, drying is mixed with the microorganism fodder additive that every gram contains hundred million active genus bacillus of 5-8.
Use above-mentioned manufacture method can make the bacillus cereus microorganism fodder additive respectively, the Bacillus licheniformis microorganism fodder additive, ring-type gemma bar bacillus microorganism fodder additive, subtilis microorganism fodder additive, bacillus firmus microorganism fodder additive and bacillus megaterium microorganism fodder additive.The purposes of each additive and consumption are pressed the kind of livestock and poultry, the etap, use different culture additive agents, and addition adds in the 0.1-0.15% ratio of feed weight.It is identical to add more than a kind each microorganism fodder additive weight of microorganism fodder additive, and gross weight is the 0.1-0.15% of feed weight
1. the microorganism fodder additive of piglet is with Bacillus licheniformis and 2 kinds of bacillus cereuses or only use a kind of microorganism fodder additive of bacillus cereus;
2. the microorganism fodder additive bacillus cereus of growing and fattening pigs, 3 kinds of microorganism fodder additives of subtilis and bacillus firmus are perhaps used 2 kinds of microorganism fodder additives of Bacillus licheniformis and subtilis;
3. the microorganism fodder additive of carp is with Bacillus licheniformis, subtilis and 3 kinds of microorganism fodder additives of Bacillus circulans;
4. the microorganism fodder additive of grass carp is with Bacillus circulans and 2 kinds of microorganism fodder additives of subtilis;
5. the microorganism fodder additive of fryer is with bacillus firmus and 2 kinds of microorganism fodder additives of bacillus cereus;
6. the microorganism fodder additive of meat duck is with Bacillus licheniformis and 2 kinds of microorganism fodder additives of bacillus megaterium.
7. the microorganism fodder additive of ox, sheep and rabbit is used Bacillus licheniformis, 3 kinds of microorganism fodder additives of subtilis and bacillus cereus.
The microorganism fodder additive of manufacturing of the present invention has the following advantages compared to existing technology:
1. have no side effect, noresidue, can guarantee meat product health and human consumer's health;
2. improve growth of animals or poultry speed, improve efficiency of feed utilization.
3. bacterial classification is easy to get, and manufacture method is simple and easy, and use is extensive.
Embodiment one:
In seeding tank, inject broth culture, inoculate hundred million active bacillus cereuses of 2-3 under 37 ℃ of conditions by every milliliter of broth culture, cultivated 12-24 hour, every milliliter contains 25-30 more than hundred million during active genus bacillus in detecting nutrient solution, change over to and produce jar interior a cultivation, produce the substratum composition and the compound method of jar and produce jar at first the tap water that purifies being put into, press aqueous solution weight again and add peptone 2%, extractum carnis 0.5%, glucose 0.3%, edible salt 0.5%, transfer PH7-7.2, stir, 120 ℃ autoclaving 40-60 minute, can use when being cooled to 30-32 ℃.Culture temperature in producing jar is 28-32 ℃, stir the number of times per minute 80-150 time, cultivated 24-48 hour, detect when the gemma production rate reaches 85-95% in the nutrient solution, to produce a jar interior nutrient solution and change 15000 rev/mins of high speed centrifugation device separates over to, remove supernatant liquor, collect thalline, then with thalline in 1 to 4 ratio uniform mixing in food starch, through 70-80 ℃ of drying and grinding repeatedly, cross 80 mesh sieve holes and make the bacterium powder, again with bacterium powder (30%), glucose (10%), starch (60%) ratio is made the microorganism fodder additive that every gram contains hundred million active bacillus cereuses of 5-8.
Use aforesaid method can make subtilis microorganism fodder additive and bacillus firmus microorganism fodder additive equally.
With bacillus cereus microorganism fodder additive and subtilis microorganism fodder additive and bacillus firmus microorganism fodder additive mixed by 1: 1: 1, and then be added in the feed by the 0.1-0.15% of feed weight and mix, the raising growing and fattening pigs show: day weight gain improves 10-12% than control group, efficiency of feed utilization improves 8-9% than control group, has better effect under the feed low nutrient level.
Embodiment two:
The difference of the manufacture method of the manufacture method of present embodiment and embodiment one is to produce jar interior nutrient solution without the high speed centrifugation device separates, also without being mixed and made into the operation of bacterium powder with starch, and directly with its nutrient solution and glucose, starch mixes, dry, make every gram and contain hundred million active genus bacillus microorganism fodder additive pulvis of 5-8, all the other are identical with the manufacture method of embodiment one, present embodiment specifically makes Bacillus licheniformis microorganism fodder additive and subtilis microorganism fodder additive, then by after 1: 1 the mixed, ratio in feed weight 0.1-0.15% adds the feed mixing again, be used to raise carp, its result shows that test group has a net increase of multiple than control group output and improves 11.9-51.2%, reduces feed coefficient 4.74-29.8%
Claims (5)
1, a kind of manufacture method of microorganism fodder additive, inoculate at seeding tank, in producing jar with a kind of in 6 kinds of the bacillus cereuses, Bacillus licheniformis, Bacillus circulans, subtilis, bacillus firmus, bacillus megaterium, cultivate breeding, it is characterized in that when jar gemma production rate that the breeding of an endospore bacillus generates reaches 85%-95%, mixing, drying, grinding be hybridly prepared into the microorganism fodder additive that every gram contains hundred million active genus bacillus of 5-8 with glucose, starch after sieving, make the bacterium powder with starch; Perhaps directly with produce jar gemma nutrient solution of endospore bacillus breeding generation with glucose, starch, mix, drying is mixed with the microorganism fodder additive that every gram contains hundred million active genus bacillus of 5-8.
2,, it is characterized in that the seeding tank inoculum density is hundred million viable bacterias of every milliliter of inoculation of medium 2-3 according to the manufacture method of the described microorganism fodder additive of claim 1; Producing a jar inoculum density is hundred million viable bacterias of per 100 milliliters of inoculation of medium 80-100.
3, according to the manufacture method of claim 1,2 described microorganism fodder additives, it is characterized in that the substratum in the seeding tank is a meat soup: the substratum in producing jar consists of peptone 2%, extractum carnis 0.5%, glucose 0.3%, edible salt 0.5%, all the other are the aqueous solution, PH7-7.2.
4, according to right 3 described microorganism fodder additive manufacture method, it is characterized in that genus bacillus employing jar interior deep ventilation and stir while fermentation culture mode, the seed tank culture temperature is 32-37 ℃, producing a jar culture temperature is 28-32 ℃, and it is 80-150 time that a production jar interior per minute stirs number of times.
5, the 0.1-0.15% that the microorganism fodder additive of producing according to claim 1, its purposes are pressed feed weight adds microorganism fodder additive; Add microorganism fodder additive more than a kind, each microorganism fodder additive weight is identical, and gross weight is the 0.1-0.15% of feed
A. the microorganism fodder additive of piglet is with Bacillus licheniformis and 2 kinds of bacterial classifications of bacillus cereus or only use a kind of bacterial classification microorganism fodder additive of bacillus cereus;
B. the microorganism fodder additive of growing and fattening pigs is with bacillus cereus and subtilis and 3 kinds of bacterial classifications of bacillus firmus, microorganism fodder additive or with Bacillus licheniformis and 2 kinds of bacterial classification microorganism fodder additives of subtilis;
C. the microorganism fodder additive of carp is with Bacillus licheniformis, subtilis and 3 kinds of bacterial classification microorganism fodder additives of Bacillus circulans;
D. the microorganism fodder additive of grass carp is with Bacillus circulans and 2 kinds of bacterial classification microorganism fodder additives of subtilis;
E. the microorganism fodder additive of fryer is with bacillus firmus and 2 kinds of bacterial classification microorganism fodder additives of bacillus cereus;
F. the microorganism fodder additive of meat duck is with Bacillus licheniformis and 2 kinds of bacterial classification microorganism fodder additives of bacillus megaterium;
G. the microorganism fodder additive of ox, sheep and rabbit is used Bacillus licheniformis, 3 kinds of bacterial classification microorganism fodder additives of subtilis and bacillus cereus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93110883A CN1089442A (en) | 1993-01-03 | 1993-01-03 | The microorganism fodder additive Manufacturing approach and use |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93110883A CN1089442A (en) | 1993-01-03 | 1993-01-03 | The microorganism fodder additive Manufacturing approach and use |
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| CN1089442A true CN1089442A (en) | 1994-07-20 |
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| CN93110883A Pending CN1089442A (en) | 1993-01-03 | 1993-01-03 | The microorganism fodder additive Manufacturing approach and use |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101190003B (en) * | 2006-12-01 | 2010-12-01 | 北京东方联鸣科技发展有限公司 | High-efficiency biological active fodder additives products and producing method and application thereof |
| CN1989831B (en) * | 2005-12-28 | 2011-06-08 | 上海创博生态工程有限公司 | Microorganism feed addictive improving reproduction ability of boar and making method thereof |
| CN102334610A (en) * | 2011-09-05 | 2012-02-01 | 李文有 | Antibiotic-free environment-friendly biological feed and production method thereof |
| CN103005163A (en) * | 2012-12-29 | 2013-04-03 | 青岛农业大学 | Compound microorganism preparation for aquatic product |
| CN103156061A (en) * | 2013-04-15 | 2013-06-19 | 山东德州神牛药业有限公司 | Compound microbial active additive |
| CN103255082A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding finishing pigs and application thereof |
| CN104489276A (en) * | 2014-12-29 | 2015-04-08 | 邳州市小河科技发展有限公司 | Rabbit biological feed additive |
| CN106720942A (en) * | 2016-12-15 | 2017-05-31 | 上海创博生态工程有限公司 | A kind of additive for microbe feedstuff for improving Eriocheir sinensis meat and preparation method thereof |
-
1993
- 1993-01-03 CN CN93110883A patent/CN1089442A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1989831B (en) * | 2005-12-28 | 2011-06-08 | 上海创博生态工程有限公司 | Microorganism feed addictive improving reproduction ability of boar and making method thereof |
| CN101190003B (en) * | 2006-12-01 | 2010-12-01 | 北京东方联鸣科技发展有限公司 | High-efficiency biological active fodder additives products and producing method and application thereof |
| CN102334610A (en) * | 2011-09-05 | 2012-02-01 | 李文有 | Antibiotic-free environment-friendly biological feed and production method thereof |
| CN103005163A (en) * | 2012-12-29 | 2013-04-03 | 青岛农业大学 | Compound microorganism preparation for aquatic product |
| CN103255082A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding finishing pigs and application thereof |
| CN103156061A (en) * | 2013-04-15 | 2013-06-19 | 山东德州神牛药业有限公司 | Compound microbial active additive |
| CN103156061B (en) * | 2013-04-15 | 2014-09-17 | 山东德州神牛药业有限公司 | Compound microbial active additive |
| CN104489276A (en) * | 2014-12-29 | 2015-04-08 | 邳州市小河科技发展有限公司 | Rabbit biological feed additive |
| CN106720942A (en) * | 2016-12-15 | 2017-05-31 | 上海创博生态工程有限公司 | A kind of additive for microbe feedstuff for improving Eriocheir sinensis meat and preparation method thereof |
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