CN108864131A - A kind of extracting method that viscid germander herb is fixed - Google Patents
A kind of extracting method that viscid germander herb is fixed Download PDFInfo
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- CN108864131A CN108864131A CN201810694484.2A CN201810694484A CN108864131A CN 108864131 A CN108864131 A CN 108864131A CN 201810694484 A CN201810694484 A CN 201810694484A CN 108864131 A CN108864131 A CN 108864131A
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- CN
- China
- Prior art keywords
- viscid germander
- germander herb
- fixed
- viscid
- herb
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/10—Spiro-condensed systems
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides a kind of extracting method that viscid germander herb is fixed, the fixed preparation method of the viscid germander herb includes the following steps：(1) viscid germander herb Chinese herbal medicine is taken, physical crushing is carried out；(2) it is flowed back, is stripped with ethanol solution；(3) extract is taken to be concentrated；(4) concentrate is eluted using sephadex LH-20 pillar, methanol；(5) condensing crystallizing is carried out to eluent；It obtains viscid germander herb and determines extract；Reach 98.72% using the fixed purity of viscid germander herb made from this method；Moreover, extraction process of the present invention is simple, elution is carried out using sephadex LH-20 column and replaces carrying out elution processing using traditional silicagel column in existing technology, substantially increases elution efficiency, and reduce solvent usage, more saving cost.
Description
Technical field
The present invention relates to effective ingredients in plant extracts, and in particular to the extracting method fixed to viscid germander herb.
Background technique
Viscid germander herb is fixed, English name：tencvidin；It is mainly extracted from viscid germander herb, existing extracting mode is using tradition
Silicagel column chromatographed, the fixed purity of viscid germander herb for needing to consume a large amount of organic solvent, and efficiency is extremely low, and obtaining
It is not high.Can be simple and direct the invention is intended to develop one kind, consumption solvent is few, and the high viscid germander herb of product purity determines extracting method.
Summary of the invention
In order to solve to need to consume a large amount of using traditional silica gel column chromatography during existing viscid germander herb extracts surely
Solvent, and extraction process is complicated, and the not high problem of purification purity, the present invention provides a kind of easy-to-use viscid germander herbs to mention surely
Take method；Including following content：
A kind of extracting method that viscid germander herb is fixed, the fixed extracting method of the viscid germander herb include the following steps：
(1) viscid germander herb Chinese herbal medicine is taken, physical crushing is carried out；
(2) it is flowed back, is stripped with ethanol solution；
(3) extract is taken to be concentrated；
(4) concentrate is eluted using sephadex LH-20 pillar, methanol,；
(5) condensing crystallizing is carried out to eluent；It obtains viscid germander herb and determines extract.
Preferably, the ethanol solution used in the fixed extracting method step (2) of the viscid germander herb is 3 liter 95% of second
Alcoholic solution.
Preferably, the fixed extracting method of the viscid germander herb includes the following steps：
(1) 1 kilogram of viscid germander herb Chinese herbal medicine is taken, physical crushing is carried out；
(2) it is flowed back, is extracted 2 times with 3 liter 95% of ethanol solution；
(3) extract is taken to be concentrated to dryness；
(4) concentrate is used into sephadex LH-20【500 grams】, 7 centimetres of column diameter (diameter), 2 liters of anhydrous methanols carry out
Elution,；
(5) it collects eluent part of the purity greater than 98% and carries out condensing crystallizing, obtain viscid germander herb and determine extract.
Reach 98.72% using the fixed purity of viscid germander herb made from this method；Moreover, extraction process of the present invention is simple, adopt
With using sephadex LH-20【500 grams】, 7 centimetres of columns of column diameter (diameter) carry out elution replace existing technology in using tradition
Silicagel column carries out elution processing, substantially increases elution efficiency, and reduce solvent usage, more saving cost.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, following embodiment be explanation of the invention and
The invention is not limited to following embodiments.
Embodiment：
A kind of extracting method that viscid germander herb is fixed is present embodiments provided, is included the following steps：
(1) 1 kilogram of viscid germander herb Chinese herbal medicine is taken, physical crushing is carried out；
(2) it is flowed back, is extracted 2 times with 3 liter 95% of ethanol solution；
(3) extract is taken to be concentrated to dryness；
(4) concentrate is used into sephadex LH-20【500 grams】, 7 centimetres of column diameter (diameter), 2 liters of anhydrous methanols carry out
Elution,；
(5) it collects eluent part of the purity greater than 98% and carries out condensing crystallizing, obtain 9 Keshan wrinkled giant hyssops and determine extract.This
The fixed purity of the viscid germander herb that method obtains reaches 98.72%；Molecular weight：328；White crystalline powder；Fusing point：215℃；Dissolution
Property：It is dissolved in methanol, ethyl alcohol, ethyl acetate is slightly soluble in petroleum ether, stability not soluble in water：It is stable at room temperature, it please set and be protected from light yin
Liang Chu；TLC detection method：Petroleum ether：Acetone=10:3, vanillic aldehyde baking colour developing；HPLC detection method：Acetonitrile：Water=60:
40, Detection wavelength 238nm.
In addition, it should be noted that, the specific embodiments described in this specification, raw material, name of product etc. can be with
It is different.The equivalent or simple change that all principles described according to the invention patent design are done, is included in the guarantor of the invention patent
It protects in range.Those skilled in the art can make various modifications to described specific embodiment
Or supplement or be substituted in a similar manner, without departing from structure of the invention or surmount defined in the claims
Range is within the scope of protection of the invention.
Claims (3)
1. a kind of extracting method that viscid germander herb is fixed, it is characterised in that：The fixed extracting method of the viscid germander herb includes the following steps：
(1) viscid germander herb Chinese herbal medicine is taken, physical crushing is carried out；
(2) it is flowed back, is stripped with ethanol solution；
(3) extract is taken to be concentrated；
(4) concentrate is eluted using sephadex LH-20 pillar, methanol,；
(5) condensing crystallizing is carried out to eluent；It obtains viscid germander herb and determines extract.
2. the fixed extracting method of viscid germander herb according to claim 1, it is characterised in that：Use in the step (2)
Ethanol solution be 3 liter 95% of ethanol solution.
3. the fixed extracting method of viscid germander herb according to claim 1, it is characterised in that：The extracting method includes as follows
Step：
(1) 1 kilogram of viscid germander herb Chinese herbal medicine is taken, physical crushing is carried out；
(2) it is flowed back, is extracted 2 times with 3 liter 95% of ethanol solution；
(3) extract is taken to be concentrated to dryness；
(4) concentrate is used into sephadex LH-20【500 grams】, 7 centimetres of column diameter (diameter), 2 liters of anhydrous methanols are washed
It is de-,；
(5) it collects part of the purity greater than 98% and carries out condensing crystallizing, obtain viscid germander herb and determine extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810694484.2A CN108864131A (en) | 2018-06-29 | 2018-06-29 | A kind of extracting method that viscid germander herb is fixed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810694484.2A CN108864131A (en) | 2018-06-29 | 2018-06-29 | A kind of extracting method that viscid germander herb is fixed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108864131A true CN108864131A (en) | 2018-11-23 |
Family
ID=64297187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810694484.2A Pending CN108864131A (en) | 2018-06-29 | 2018-06-29 | A kind of extracting method that viscid germander herb is fixed |
Country Status (1)
| Country | Link |
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| CN (1) | CN108864131A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771394A (en) * | 2019-02-28 | 2019-05-21 | 广东岭南职业技术学院 | A kind of viscid germander herb anti-inflammatory plastics and preparation method thereof |
| CN116173124A (en) * | 2022-12-14 | 2023-05-30 | 宏铖同创(山东)医药科技有限公司 | Traditional Chinese medicine composition for treating digestive system tumor postoperative gastroparesis and preparation system |
-
2018
- 2018-06-29 CN CN201810694484.2A patent/CN108864131A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771394A (en) * | 2019-02-28 | 2019-05-21 | 广东岭南职业技术学院 | A kind of viscid germander herb anti-inflammatory plastics and preparation method thereof |
| CN109771394B (en) * | 2019-02-28 | 2022-03-15 | 广东岭南职业技术学院 | Agastache rugosus anti-inflammation film coating agent and preparation method thereof |
| CN116173124A (en) * | 2022-12-14 | 2023-05-30 | 宏铖同创(山东)医药科技有限公司 | Traditional Chinese medicine composition for treating digestive system tumor postoperative gastroparesis and preparation system |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181123 |
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| WD01 | Invention patent application deemed withdrawn after publication |