CN108690803A - A kind of device for formulating for microbial mineralization culture medium - Google Patents
A kind of device for formulating for microbial mineralization culture medium Download PDFInfo
- Publication number
- CN108690803A CN108690803A CN201810808829.2A CN201810808829A CN108690803A CN 108690803 A CN108690803 A CN 108690803A CN 201810808829 A CN201810808829 A CN 201810808829A CN 108690803 A CN108690803 A CN 108690803A
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- Prior art keywords
- blender
- rod
- bottle
- culture medium
- mixer
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000001963 growth medium Substances 0.000 title claims abstract description 30
- 230000033558 biomineral tissue development Effects 0.000 title claims abstract description 16
- 230000000813 microbial effect Effects 0.000 title claims abstract description 15
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 238000002479 acid--base titration Methods 0.000 claims abstract description 10
- 230000007246 mechanism Effects 0.000 claims abstract description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 15
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 238000002360 preparation method Methods 0.000 abstract description 6
- 238000004140 cleaning Methods 0.000 abstract description 4
- 230000007812 deficiency Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 11
- 230000006698 induction Effects 0.000 description 9
- 229910052500 inorganic mineral Inorganic materials 0.000 description 9
- 239000011707 mineral Substances 0.000 description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 9
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 9
- 230000008569 process Effects 0.000 description 5
- 239000007640 basal medium Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 208000037516 chromosome inversion disease Diseases 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000010977 unit operation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
Landscapes
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of device for formulating for microbial mineralization culture medium, belong to the preparation technique field of culture medium.Which solve prepare the technological deficiencies such as cumbersome in the prior art.It includes the lateral body of rod, the vertical body of rod, pedestal, the first mixer, the second mixer, pH detectors, acid base titration pipe, removable Jie Ye mechanisms and the reserve tank equipped with saturation KCl, the lateral body of rod is mutually parallel with pedestal, and pedestal is located at the lower section of the lateral body of rod, the first mixer, the second mixer, pH detectors, acid base titration pipe and the reserve tank equipped with saturation KCl are each attached on the lateral body of rod;First mixer includes the first bottle and the first blender, second mixer includes the second bottle and the second blender, second blender is located in the second bottle, and the first blender and the second blender share a rocking arm, makes the first blender and the second blender by rotation rocking arm while stirring.Device for formulating of the present invention is removable to be filled, easy cleaning, can directly be sterilized.
Description
Technical field
The present invention relates to the preparation technique fields of culture medium, and in particular to a kind of microbial mineralization culture medium preparing device.
Background technology
Microorganism induction mineral deposition is a hot spot of world today's biomineralization area research, illustrates micro- life emphatically
The instrumentality of effect of the object in mineral forming process, microorganisms class is biological to the carbon and other elements in region or the whole world
The influence of geochemical cycle.Microorganism induction mineral deposition is formed due to growth and the metabolism of microorganism, is led to
The research to microorganism induction mineral is crossed not only it will be seen that the course of biological evolution, additionally aids the mineral from microorganism induction
The chemical element index of middle extraction effectively reflection weather and environmental change, to carry out geologic climate and palaeoenvironmental reconstruction.Most
Microorganism induction mineral and its Significance for Environment have become the research field that have developed rapidly in close 20 years.More and more people
Begin to focus on this field of microorganism induction mineral, more and more scholars begin one's study it is various under the conditions of microorganism induction mineral
Result.
Operating personnel prepare culture medium when doing experiment of the microorganism induction at mine, or using traditional method at present,
Conventional method does not form a complete preparation system, therefore its process for preparation and its cumbersome, and the prior art is at present
There is no the device for formulating of complete set, it is contemplated that following defect existing for culture medium configuration process under traditional difference Mg/Ca:
The first, culture medium configuration process is extremely complex, and each similar drug of addition is easy to cause confusion too much, and technological process is too
It is lengthy and tedious;
The second, raw material is easy to be pasted onto on container inner wall during unit operation, easily loses more raw material, and experiment is caused to miss
Difference, and it is easy leakage in the bottle inversion stage, and too many, easy dye miscellaneous bacteria is operated in preparation process.
Thus, it is necessary to study a kind of device for formulating for microbial mineralization culture medium.
Invention content
The purpose of the present invention is to provide a kind of device for formulating for microbial mineralization culture medium, the device for formulating is readily removable
Easily dress, easy cleaning can sterilize, reduce with the extraneous chance contacted, reduce because prepare culture medium and by the risk of microbiological contamination,
It is more convenient that culture medium is configured under different Mg/Ca solution environmentals.
Its technical solution includes:
A kind of device for formulating for microbial mineralization culture medium comprising the lateral body of rod, the vertical body of rod, pedestal, first
Mixer, the second mixer, pH detectors, acid base titration pipe, removable Jie Ye mechanisms and the reserve tank equipped with saturation KCl, institute
The lateral body of rod stated is mutually parallel with the pedestal, and the pedestal is located at the lower section of the lateral body of rod, the vertical body of rod
It is connected to the homonymy of the lateral body of rod and the pedestal;
First mixer, the second mixer, pH detectors, acid base titration pipe and the reserve tank equipped with saturation KCl
It is each attached on the lateral body of rod;
First mixer includes the first bottle and the first blender, is used to place basis in first bottle
Culture medium, second mixer include the second bottle and the second blender, are used to place in second bottle
NaHCO3And Na2CO3Solution, second blender are located in second bottle, and first blender and the second stirring
Device shares a rocking arm, makes first blender and the second blender by the rotation rocking arm while stirring;Described
Filter is additionally provided in two bottles, second bottle is also connected with vacuum pump by a rubber catheter;
It is both provided with first outlet and second outlet in the bottom of first bottle, the second bottle, and described first goes out
Mouthful, the adjacent place of second outlet be provided with the first control valve and the second control valve, when vacuum pump be in open state and second controls
When valve processed is opened, the NaHCO in the second bottle after filtering3And Na2CO3Solution is discharged through second outlet;
The removable Jie Ye mechanisms include a vertically arranged supporting rod and the support above the supporting rod
Disk is horizontally arranged with a sliding rail on the pedestal, and the bottom of the supporting rod is movably connected on the sliding rail, described
Setting is there are six groove on pallet, and each groove is interior to place a conical flask, and the conical flask is interior for holding through described first
The liquid of the outlet discharge of the reserve tank of outlet, the outlet of second outlet acid base titration pipe and saturation KCl.
As a preferred solution of the present invention, logger is provided on the vertical body of rod, the logger
There are four control buttons for setting on panel, and when pressing one of control button, record is this time prepared and completed.
As another preferred embodiment of the present invention, first blender, the second blender include paddle, stir
Bar and gear are mixed, the paddle is located on the stirring rod, and the gear is arranged at the top of the stirring rod, and with
The rocking arm intermeshing.
Further, the bottom of the supporting rod is flexibly connected by three balls with the sliding rail realization.
Further, the pH detectors include electrode tip, and the electrode tip is placed on the deposit equipped with saturation KCl
In case, when needing to measure pH, the electrode tip is taken out.
Further, the filter includes filter membrane, and the filter membrane is located at the middle part of second bottle.
Compared with prior art, present invention offers following advantageous effects:
Device for formulating of the present invention is integrally standardized, simple in structure, removable to fill, easy cleaning, can directly sterilize, in different Mg/
It is more convenient that culture medium is configured under Ca solution environmentals, is saved a large amount of time and manpower and materials, has been saved experimentation cost.
Description of the drawings
The present invention will be further described below in conjunction with the accompanying drawings:
Fig. 1 is the structure diagram of device for formulating of the present invention;
Fig. 2 is the structural schematic diagram of the first blender of the present invention, the second blender and rocking arm;
In figure, 1,2,23,24,38,39- fixed rings, 3,4,25,26- gears, 5, rocking arm, 6, basal medium dispensing throws
Entrance, 7,28, paddle, the 8, first mixer, the 9, first control valve, 10, first outlet, 11, pallet, 12, handle, 13,14,
15,16,17,18- conical flasks, 19, supporting rod, 20, slideway base, 21, ball, 22, slideway, 27, NaHCO3Drug is launched
Mouthful, 29, filter, 30, filter membrane, the 31, second mixer, the 32, second control valve, 33, second outlet, 34, vacuum pump, 35,
Rubber catheter, 36, pedestal, 37, support plate, 40, pH meter, 41, electrode tip, 42, KCl solution reserve tanks, 43, third control valve,
44,45- conduits, 46, electrode tip containing tube, 47, clip, 48, clip, 49, scale, 50, scale, 51, acid buret, 52,
Base buret, 53, logger.
Specific implementation mode
The present invention proposes a kind of device for formulating for microbial mineralization culture medium, in order to make advantages of the present invention, skill
Art scheme is clearer, clear, elaborates to the present invention with reference to specific embodiment.
In conjunction with shown in Fig. 1 and Fig. 2, a kind of device for formulating for microbial mineralization culture medium of the invention, fixed ring 1,2,
23,24,38,39, gear 3,4,25,26, Rocker arm 5, basal medium dispensing input port 6, paddle 7,28, the first mixer 8,
First control valve 9, the second control valve 32, third control valve 43, conduit 44 and 45,51,52, culture medium outlet 10, rotary-tray
11, rotary-tray handle 12, conical flask 13,14,15,16,17,18, basetray 19, slideway base 20, pulley 21, slideway
22,Na2CO3,NaHCO3Drug dispensing port 27, filter membrane 30, filter 29, detachable conical flask 31, Na2CO3,NaHCO3Outlet
33, vacuum pump 34, rubber catheter 35, pedestal 36, support plate 37, pH meter 40, electrode tip 41, saturation KCl solution reserve tank 42, lead
Pipe 44,45, electrode tip containing tube 46, clip 47,48, acid buret 51 (carrying scale 49 above), 52 (band of base buret
Have scale 50) and logger 53.
First mixer includes the first bottle and the first blender, is used to place basal medium in the first bottle, i.e., will
The dispensing of culture medium is added by basal medium dispensing input port 6 in proportion, and the second mixer 31 includes the second bottle and the
Two blenders, the second bottle are interior for placing NaHCO3And Na2CO3Solution, the second blender are located in the second bottle, Na2CO3,
NaHCO3Pass through NaHCO3Drug dispensing port 27 is added, and the first blender, the second blender include paddle, stirring rod and tooth
Wheel, paddle are located on stirring rod, and gear is arranged at the top of stirring rod, and are intermeshed with rocking arm;And first blender and
Second blender shares a rocking arm, makes the first blender and the second blender by rotation rocking arm while stirring;Second bottle
It is inside additionally provided with filter 29, second bottle is also connected with vacuum pump 34 by a rubber catheter;
Supporting rod 19 is arranged in the lower section of above-mentioned pallet, supports and it is driven to be rotated, below supporting rod
For slideway base 20, a slideway 22 (sliding rail) is horizontally arranged on slideway base 20, the bottom of supporting rod is movably connected in cunning
On rail, preferably the bottom of supporting rod is flexibly connected by three balls 21 with the sliding rail realization.Six are provided on pallet
A groove, places a conical flask in each groove, for holding through first outlet, second outlet acid base titration pipe in conical flask
The liquid of the outlet discharge of the reserve tank of outlet and saturation KCl;Rocker arm 5 is rotated to the rotation with moving gear 4 and 25, in gear
Under the action of 4 and 25, the rotation with moving gear 3 and 25, the rotation of gear 3 and 26 to drive the stirring of paddle 7 and 28,
Under the stirring of paddle 7, after culture medium is stirred evenly, rotary-tray 11 is slided into the bottom of the first blending bin 8, opens first
Control valve 9, by first outlet 10, rotation rotary-tray flows into conical flask 13,14,15,16,17,18 successively, for convenience of grasping
Make, handle 12 can be set on pallet, rotary-tray 11 is disassembled from pedestal 20 later, is sterilized.It beats this moment
Vacuum pump 34 is opened, under the action of vacuum pump 34, Na2CO3The sterilizing that is filtered slowly is dripped into conical flask 31.It will be sterilized
Conical flask is taken out afterwards be attached to and be cooled to room temperature on pedestal after, open the second control valve 32, be slowly added dropwise according to required reagent requirement
Na2CO3Solution is sequentially added from second outlet 33 into 6 conical flasks 13,14,15,16,17,18.NaHCO3The dropwise addition of solution
It is then to repeat aforesaid operations.
For the ease of controlling third control valve 43 is provided on the pipe below saturation KCl solution reserve tank 42.
In all culture medium and Na2CO3,NaHCO3After being linked into conical flask, by pH meter 40 in each conical flask
Culture medium solution carry out pH value measurement, electrode tip 41 is taken out from electrode tip containing tube 46, by the rotation with conical flask
The disk 11 that asks slides into the bottom of pH meter 40, electrode tip is put to the measurement that pH value is carried out in conical flask into, if properly after continued access bacterium
Culture, if the improper lower section progress acid base titration that rotary-tray 11 is gone to acid buret 51, base buret 52, directly
Until being transferred to suitable pH value.
Package unit of the present invention is easy to operate, easy cleaning, for more fast and convenient preparation microorganism in different Mg/Ca
Culture medium used, greatly reduces operation complexity, saves manpower and materials when lower induction mineral;Save time and essence
Power reduces experimental cost.
It uses for reference the prior art and can be realized in the part that do not addressed in the present invention.
It should be noted that any equivalent way that those skilled in the art are made under the introduction of this specification, or
Obvious variant should all be within the scope of the present invention.
Claims (6)
1. a kind of device for formulating for microbial mineralization culture medium comprising the lateral body of rod, the vertical body of rod, pedestal, first mix
Clutch, the second mixer, pH detectors, acid base titration pipe, removable Jie Ye mechanisms and the reserve tank equipped with saturation KCl, it is special
Sign is:
The lateral body of rod is mutually parallel with the pedestal, and the pedestal is located at the lower section of the lateral body of rod, described perpendicular
The homonymy of the lateral body of rod and the pedestal is connected to the body of rod;
First mixer, the second mixer, pH detectors, acid base titration pipe and reserve tank equipped with saturation KCl are solid
It is scheduled on the lateral body of rod;
First mixer includes the first bottle and the first blender, is used to place basic culture in first bottle
Base, second mixer include the second bottle and the second blender, are used to place NaHCO in second bottle3With
Na2CO3Solution, second blender are located in second bottle, and first blender and the second blender share one
Rocking arm makes first blender and the second blender by the rotation rocking arm while stirring;In second bottle
It is additionally provided with filter, second bottle is also connected with vacuum pump by a rubber catheter;
It is both provided with first outlet and second outlet, and the first outlet, in the bottom of first bottle, the second bottle
The adjacent places of two outlets are provided with the first control valve and the second control valve, when vacuum pump is in open state and the second control valve is opened
Qi Shi, the NaHCO in the second bottle after filtering3And Na2CO3Solution is discharged through second outlet;
The removable Jie Ye mechanisms include a vertically arranged supporting rod and the pallet above the supporting rod, institute
A sliding rail is horizontally arranged on the pedestal stated, the bottom of the supporting rod is movably connected on the sliding rail, in the pallet
Upper setting places a conical flask there are six groove in each groove, in the conical flask for hold through the first outlet,
The liquid of the outlet of second outlet acid base titration pipe and the outlet discharge of the reserve tank of saturation KCl.
2. a kind of device for formulating for microbial mineralization culture medium according to claim 1, it is characterised in that:Described
The vertical body of rod on be provided with logger, setting is there are four control button on the panel of the logger, when pressing therein one
Record is this time prepared and is completed when a control button.
3. a kind of device for formulating for microbial mineralization culture medium according to claim 1, it is characterised in that:Described
First blender, the second blender include paddle, stirring rod and gear, and the paddle is located on the stirring rod,
The gear is arranged at the top of the stirring rod, and is intermeshed with the rocking arm.
4. a kind of device for formulating for microbial mineralization culture medium according to claim 1, it is characterised in that:Described
The bottom of supporting rod is flexibly connected by three balls with the sliding rail realization.
5. a kind of device for formulating for microbial mineralization culture medium according to claim 1, it is characterised in that:Described
PH detectors include electrode tip, and the electrode tip is placed in the reserve tank equipped with saturation KCl, will when needing to measure pH
The electrode tip takes out.
6. a kind of device for formulating for microbial mineralization culture medium according to claim 1, it is characterised in that:Described
Filter includes filter membrane, and the filter membrane is located at the middle part of second bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810808829.2A CN108690803A (en) | 2018-07-23 | 2018-07-23 | A kind of device for formulating for microbial mineralization culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810808829.2A CN108690803A (en) | 2018-07-23 | 2018-07-23 | A kind of device for formulating for microbial mineralization culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108690803A true CN108690803A (en) | 2018-10-23 |
Family
ID=63850793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810808829.2A Pending CN108690803A (en) | 2018-07-23 | 2018-07-23 | A kind of device for formulating for microbial mineralization culture medium |
Country Status (1)
| Country | Link |
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| CN (1) | CN108690803A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113413803A (en) * | 2021-06-22 | 2021-09-21 | 吉林建筑大学 | A configuration device for microorganism mineralize mineralization culture medium |
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2018
- 2018-07-23 CN CN201810808829.2A patent/CN108690803A/en active Pending
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| CN113413803A (en) * | 2021-06-22 | 2021-09-21 | 吉林建筑大学 | A configuration device for microorganism mineralize mineralization culture medium |
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Application publication date: 20181023 |