CN108553371A - Have effects that skin care compositions that antiallergic is releived and preparation method and application - Google Patents
Have effects that skin care compositions that antiallergic is releived and preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
The invention discloses a kind of skin care compositions and the preparation method and application thereof having effects that antiallergic is releived.The raw material that the skin care compositions is matched by following weight parts is prepared：1 10 parts of Japan cypress, 1 10 parts of white willow, 1 10 parts of hundred golden flower, 1 15 parts of rheum rhabarbarum, 1 10 parts of river paddy, 1 10 parts of plantain, 1 10 parts of rhodiola root.The present invention be verified by experiments can in antagonism cosmetics surfactant, preservative irritation, there is effective the effect of inhibiting allergy and itching-relieving efficacies, also having both skin whitening, moisturizing, play the role of skin barrier.
Description
Technical field
The invention belongs to cosmetic fields, more particularly to a kind of skin care compositions and preparation side having effects that antiallergic is releived
Method and application.
Background technology
The incidence of allergy is very high, and there are about the people of l/3 to suffer from anaphylactia in life for document report.According to China north
The statistics in capital area, the incidence of allergy is about 15% in population.The anaphylactia in some areas of the Pacific Ocean is even
Up to 50%.Since the 21th century, which has become one of most common global disease.The World Health Organization (WHO)
Statistics indicate that the current whole world has 22%~25% people to suffer from anaphylactia, and increased with every 10 years 23 times of speed, at present
Only just there is people's illness more than 200,000,000 in China.Therefore allergy and antiallergy, are the unavoidable severe challenges that modern faces.
Also there is sensitization specified risk material in cosmetics, such as：Surfactant, essence and flavoring agent, preservative.Consumer is to cosmetics
The stimulation and sensitive concern of initiation have reached unprecedented degree, and the safety of cosmetics is even more to come cosmetic properties
First of.
Invention content
The primary purpose of the present invention is that solving above-mentioned problem of the prior art, ensure that consumer uses the safety of product
And propose it is a kind of can be with the irritation of antagonism surfactant, essence and flavoring agent, preservative, while also having and repairing damaged skin
Effect has effects that antiallergic is releived the skin care compositions of anti-irritant.
The plant extract that another object of the present invention is to propose a kind of preparation method of above-mentioned skin care compositions and obtains
Object.
The third object of the present invention is to propose a kind of skin care item containing above-mentioned plant extracts.
The present invention utilizes advanced biotechnology, in conjunction with the theory of traditional Chinese medical science of Chinese tradition, by Japan cypress, white willow, hundred golden flower, wave
Leaf rheum officinale, Chuan Gu, plantain, rhodiola root are effectively combined in together, form compound, and as active component, further make
It is standby to be stimulated with antagonism Preservative Technology for Personal Care, surfactant at skin care formulation, also have and presses down quick, antipruritic, whitening anti-inflammatory
And other effects.
To achieve the purpose of the present invention, the present invention uses following specific technical solution：
A kind of skin care compositions for having effects that antiallergic and releiving, wherein the skin care compositions is matched by following weight parts
Raw material be made：
1-10 parts of Japan cypress, 1-10 parts of white willow, 1-10 parts of hundred golden flower, 1-15 parts of rheum rhabarbarum, paddy 1-10 parts of river, plantain 1-10
Part, 1-10 parts of rhodiola root.
The preferable weight of each raw material of composition is：
It is 2-8 parts of Japan cypress, 2-8 parts of white willow, 2-8 parts of hundred golden flower, 2-10 parts of rheum rhabarbarum, paddy 2-8 parts of river, 2-8 parts of plantain, red
2-8 parts of red-spotted stonecrop.
A kind of preparation method of above-mentioned skin care compositions, steps are as follows for the method：
(1) above-mentioned raw materials are weighed after crushing by the weight, mixing；
(2) it is that 70-90% ethyl alcohol extracts with percent by volume, the mass volume ratio g/mL of raw material and ethyl alcohol is 1：(10-
30), 70-80 DEG C of extraction 1-4h；
(3) extracting solution that step (2) obtains is cooled to room temperature (20-30 DEG C), the filtering of 80-100 mesh obtains filtrate；
(4) 1,3-BDO that original amount of alcohol is added in filtrate is miscible uniformly, evaporates the ethyl alcohol in solution, and vacuum is taken out
Filter, collect filtrate to get.
The percent by volume of ethyl alcohol is 80% in the step (2), and the mass volume ratio g/mL of raw material and ethyl alcohol is 1：20.
Vacuum filtration condition is that 1.2 μm of filter plates are spread in Buchner funnel in the step (4), is filtered by vacuum.
A kind of plant extracts for having effects that antiallergic and releiving, wherein what the extract was extracted by the above method.
Above-mentioned plant extracts has effects that in preparation in the external use skin care additive or skin care formulation that antiallergic is releived
Purposes.
A kind of skin care formulation for having effects that antiallergic and releiving, wherein the skin care formulation is by above-mentioned plant extracts and shield
Skin product field customary adjuvant is made.
A kind of cream for having effects that antiallergic and releiving, wherein the cream includes the component of following weight percentage, such as
Shown in table 1：
Table 1
| Component | Weight percent % |
| Above-mentioned plant extracts | 1-10 |
| Cetostearyl alcohol | 1-3 |
| Stereth -2 | 0.5-2 |
| Ring five dimethyl silicone polymer | 1-3 |
| Cyclohexasiloxane | 0.1-1.5 |
| The poly- trimethicone of diphenyl silicon phenyl | 0.1-2 |
| Butyrospermum parkii (BUTYROSPERMUMPARKII) fruit fat | 0.1-1 |
| Hydrogenated coco | 0.1-2 |
| Dimethyl silicone polymer | 1-2 |
| Saualane | 0.1-2 |
| Olive (OLEAEUROPAEA) fruit oil | 0.1-2 |
| Tocopherol (vitamin E) | 0.1-1 |
| Hydrolecithin | 0.1-1 |
| Methyl stearyl sodium taurocholate | 0.1-1 |
| Pentaerythritol tetra (di-tert-butyl hydroxy hydrocinnamate) | 0.1-1 |
| Rice (ORYZASATIVA) oil extracted from rice husks | 0.1-1 |
| Glycine betaine | 0.5-2.5 |
| Tribehenin essence PEG-20 esters | 0.1-1 |
| Cremophor RH40 | 0.01-0.1 |
| EDETATE SODIUM | 0.01-0.05 |
| Sodium Hyaluronate | 0.01-0.05 |
| Nephrite powder | 0.01-0.2 |
| Xanthans | 0.1-0.3 |
| Polyalcohol | 5-15 |
| Preservative | 0.05-0.2 |
| Water | Surplus |
Wherein, polyalcohol described in above-mentioned cosmetics is sorbierite, D-sorbite, pungent glycol, dipropylene glycol, methyl-prop fibre
Tie up alcohol, erythritol, maltitol, mannitol, pentanediol, Sensiva SC50, isoprene, one kind in hexylene glycol or one
Kind or more mixing.
Wherein, the preservative described in above-mentioned cosmetics is at least one of potassium sorbate, benzyl alcohol, Phenoxyethanol.
In above-mentioned cream remove plant extract beyond the region of objective existence other components, be studied by inventor and plant extracts
Match and plays the ingredient of optimum synergistic effect.
Other dosage forms can also be prepared using this plant extracts and skin care field conventional method and auxiliary material, such as essence
China, toner, lotion, spray etc..
The preparation of the present invention recommends method and is：After daily face cleaning, human skin surface is imposed on smearing method, gently
Massage is until absorb.
The beneficial outcomes of the present invention：
The present invention, which is verified by experiments, can inhibit DNA Damage and leakage, increase skin-tolerant, improve cutaneous immunisation
Power and resistance prevent the generation of the damages such as skin allergy.Surface-active in the acceptable effectively antagonism cosmetics of the present invention simultaneously
The irritation of agent and preservative has effective inhibition allergy and itching-relieving efficacies, inhibits I type super quick anti-by reducing IL-6 contents
The effect for answering (immediate hypersensitivity) inhibits the work of IV type hypersensitivity (delayed allergy) by reducing IL-8 contents
With.The effect of also having both skin whitening, moisturizing, plays the role of skin barrier.Easy, preparation method letter that the raw material of the present invention obtain
Just, green safe, it is easy to be received by allergic human population, there are good application prospect and market prospects.
Specific implementation mode
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention will be made into one below
Step ground detailed description, embodiments of the present invention are not limited thereto, for not specifically specified technological parameter or condition, can refer to
Routine techniques carries out.
Seven tastes component effect according to the present invention is described below：
The Japan cypress is a kind of aiphyllium of Cupressaceae Platycladus, and Classification system is Platycladus orientalis
(Linn.) Franco, also known as cypress, arbor-vitae, arborvitae, leaf are used as medicine, acrid flavour, slightly sour hardship, cold in nature, enter the heart, liver, large intestine channel, have
It makes light of one's life by commiting suicide QI invigorating, makes us resistance to cold and heat, go arthritis with fixed pain caused by dampness, the effect of myogenic.
The white willow is Salicaceae sallow, and Classification system is Salix alba L., is used as medicine with leaf, bud or root, taste
Hardship, it is cold in nature, there is the effect of clearing heat and detoxicating, dispelling wind and eliminating dampness.
Hundred golden flower is Gentianaceae Centaurium Hill herbaceous plant, and Classification system is Centaurium pulchellum
(Swartz) Druce var.altaicum (Griseb.) Kitag.et Hara, all herbal medicine, bitter, cold nature have clear
The effect of heat, removing jaundice, cholagogic.
The rheum rhabarbarum is polygonaceae Rheum wave leaf group herbaceos perennial, Classification system Rheum
Undulatum Linn., root are used as medicine, cold in nature, bitter, enter stomach, large intestine channel, have the effect of removing toxic substances of purging heat, the cool blood row stasis of blood.
The Chuan Gu is that grass family Coix is annual or perennial herb, Classification system are Coix lacryma-jobi
Linn., benevolence is used as medicine, bitter but sweet flavor, cold in nature, nontoxic, enters spleen, bladder warp, has the effect of heat-clearing, dampness removing, invigorating the spleen, desinsection.
The plantain is Musaceae Musa herbaceos perennial, and Classification system is Musa sapientum
Linn., fruit is used as medicine, sweet in flavor；It is cold in nature, enter lung, the spleen channel, has effects that heat-clearing, moistening lung, laxation, removing toxic substances.
The rhodiola root is the full valve rhodiola root of Crassulaceae Rhodida plant, and Classification system is Rhodiola rosea
Linn., the effect of root is used as medicine, cold in nature, sweet-puckery flavor, enters the heart, lung channel, has and controls hemoptysis, spits blood, pneumonia cough, women leukorrhea.
The preparation of 1 skin care compositions of embodiment
(1) following raw materials are weighed after crushing by the weight proportion, mixing；Japan cypress 100g, white willow 100g, hundred golden flower 50g,
Rheum rhabarbarum 150g, river paddy 10g, plantain 10g, rhodiola root 50g；
(2) ethyl alcohol that percent by volume is 90% is used to extract, raw material is 1 with ethyl alcohol mass volume ratio g/mL：20,75 DEG C carry
Take 2.5h；
(3) extracting solution that step (2) obtains is cooled to room temperature 20 DEG C, the filtering of 100 mesh obtains filtrate；
(4) 1,3-BDO that original amount of alcohol is added in filtrate is miscible uniform, and rotary evaporation goes out the ethyl alcohol in solution,
1.2 μm of filter plates are spread in Buchner funnel, above-mentioned gained filtrate is filtered by vacuum, and collect filtrate up to plant extracts A.
The preparation of 2 skin care compositions of embodiment
(1) following raw materials are weighed after crushing by the weight proportion, mixing；Japan cypress 10g, white willow 50g, hundred golden flower 10g, wave
Leaf rheum officinale 80g, river paddy 100g, plantain 50g, rhodiola root 100g；
(2) ethyl alcohol that percent by volume is 70% is used to extract, raw material is 1 with ethyl alcohol mass volume ratio g/mL：30,80 DEG C carry
Take 1h；
(3) extracting solution that step (2) obtains is cooled to room temperature 30 DEG C, the filtering of 80 mesh obtains filtrate；
(4) 1,3-BDO that original amount of alcohol is added in filtrate is miscible uniform, and rotary evaporation goes out the ethyl alcohol in solution,
1.2 μm of filter plates are spread in Buchner funnel, above-mentioned gained filtrate is filtered by vacuum, and collect filtrate up to plant extracts B.
The preparation of 3 skin care compositions of embodiment
(1) following raw materials are weighed after crushing by the weight proportion, mixing；Japan cypress 50g, white willow 10g, hundred golden flower 100g,
Rheum rhabarbarum 10g, river paddy 50g, plantain 100g, rhodiola root 10g；
(2) ethyl alcohol that percent by volume is 80% is used to extract, raw material is 1 with ethyl alcohol mass volume ratio g/mL：10,70 DEG C carry
Take 4h；
(3) extracting solution that step (2) obtains is cooled to room temperature (25 DEG C), the filtering of 90 mesh obtains filtrate；
(4) 1,3-BDO that original amount of alcohol is added in filtrate is miscible uniform, and rotary evaporation goes out the ethyl alcohol in solution,
1.2 μm of filter plates are spread in Buchner funnel, above-mentioned gained filtrate is filtered by vacuum, and collect filtrate up to plant extracts C.
The component and dosage of embodiment 4-6 Shu Min creams are shown in Table 2
Table 2
Wherein, the plant extracts described in embodiment 4 is plant extracts A obtained by embodiment 1 is prepared, described more
First alcohol is sorbierite, and the preservative is potassium sorbate；Plant extracts described in embodiment 5 is prepared by embodiment 2
Obtained by plant extracts B, the polyalcohol be dipropylene glycol, the preservative be benzyl alcohol；Described in embodiment 6
Plant extracts is plant extracts C obtained by embodiment 3 is prepared, and the polyalcohol is potassium sorbate, the anti-corrosion
Agent is benzyl alcohol.
Comparative example 1-7
Comparative example 1-7 is other than plant extracts is different, remaining component and dosage are same as Example 4, wherein comparative example 1
The plant extracts is prepared by Japan cypress preparation method described in embodiment 1；Wherein plant extracts described in comparative example 2 by
White willow preparation method described in embodiment 1 is prepared；Wherein plant extracts described in comparative example 3 is by hundred golden flower embodiments 1
The preparation method is prepared；Wherein plant extracts described in comparative example 4 is by rheum rhabarbarum preparation method described in embodiment 1
It is prepared；Wherein plant extracts described in comparative example 5 is prepared by Chuan Gu preparation methods described in embodiment 1；It is wherein right
Plant extracts described in ratio 6 is prepared by plantain preparation method described in embodiment 1；Wherein plant described in comparative example 7 carries
Object is taken to be prepared by rhodiola root preparation method described in embodiment 1.
Efficacy experiments of the present invention
One, embodiments 1, embodiment 2, the evaluation of 3 gained plant extracts A, B, C antipruritic effect of embodiment
Submitted sample is calculated to the itch-threshold of allergic skin itching model, for evaluation sample itching-relieving efficacies provide experiment according to
According to.Experimental method：
(1) test medicine：Plant extracts A, B, C that embodiment 1, embodiment 2 and embodiment 3 are prepared；
(2) it is grouped：Experiment mice totally 60 is divided into 1 plant extracts A large dosages group of embodiment, middle dose group and small dose
Amount group, 2 plant extracts B large dosages group of embodiment, middle dose group and small dose group, 3 plant extracts C large dosages of embodiment
Group, middle dose group and small dose group, model control group, every group 6.
(3) dose design
Plant extracts is by test product with 0.15mL/1cm2、0.1mL/1cm2、0.05mL/1cm2Partial smearing is as big agent
Amount, middle dose group and small dose group.Model control group gives 0.1mL/1cm2Distilled water partial smearing.
(4) experimentation is first taken off the right metapedes dorsal body setae hair of mouse with depilatory cream, is uniformly applied respectively at depilation for three days on end
Corresponding dosage is smeared by test product, model control group gives distilled water smearing.It tests the 3rd day, it is appropriate that precision weighs histamine phosphate, faces use
It is preceding be made into 0.01% with distilled water, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%,
0.10% gradient concentration is spare.It will be abraded at instep depilation behind the cavy right side with coarse sandpaper, area about 1cm2, part coating 1 time again,
Drip 0.01% histamine phosphate 0.05mL in abrasion after last coating 10min, later every 3min according to 0.01%, 0.02%,
0.03%, 0.04% ... progressive concentration is 0.05mL every time.Right metapedes later is licked until there is cavy, finally to occur
Cavy turns one's head to drip the histamine phosphate total amount taken when licking right metapedes to be itch-threshold.Calculate the difference between each group itch-threshold and comparative group
Property.
(5) experimental result
The results show that after being itched using histamine phosphate cause, cavy occurs later licking right metapedes behavior.It is caused using being smeared by test product
Itch behind position, plant extracts A, B, C are remarkably improved cavy histamine phosphate itch-threshold, more significant with model control group
Difference (P<0.01P<0.05) 3, are shown in Table.
3 sample of table causes the influence (x ± s, n=6) of reaction of itching to cavy histamine phosphate
Note：Compared with model control group, * P<0.05, * * P<0.01
(6) experiment conclusion
Plant extracts A, B, C have good itching-relieving efficacies to pruitus after allergy.
Two, embodiments 1, embodiment 2, embodiment 3 gained plant extracts A, B, C inhibit skin allergy efficacy assessments
(1) test medicine：Plant extracts A, B, C is prepared in embodiment 1, embodiment 2 and embodiment 3；
(2) it is grouped：Experiment mice totally 55 is divided into 1 plant extracts A large dosages group of embodiment, middle dose group and small dose
Amount group, 2 plant extracts B large dosages group of embodiment, middle dose group and small dose group, 3 plant extracts C large dosages of embodiment
Group, middle dose group and small dose group, blank control group, model control group, every group 5.
(3) dose design
By test product with 0.15mL/1cm2、0.1mL/1cm2、0.05mL/1cm2Partial smearing is as large dosage, middle dose group
And small dose group.Blank control group and model control group give 0.1mL/1cm2Distilled water partial smearing.
(4) experimentation
First mouse back hair is taken off with depilatory cream, in addition to blank control group, remaining each group rat back chooses 3
Point, every is subcutaneously injected anti-DNP IgE0.5 μ g (0.5 μ L).After 48h, the injection of each group rat tail vein contains 4% ivens
Blue 100 μ g of DNP-HAS (100 μ L).The 1h before tail vein injection DNP-HAS, in administration each group rat back, with injection
Centered on the point of anti-DNP IgE, every smearing 2cm2The relative medicine of area.30min is put to death after being injected intravenously DNP-HAS
Rat.Blue dye skin is cut, uses 1：1 acetone-physiological saline mixed solution impregnates for 24 hours, and centrifuging and taking supernatant uses spectrophotometer
OD values are detected at 620nm.Using Evans Blue solution make standard curve, calculate every rat dorsum skin dye content and
PCA response inhabitation rates.
(5) experimental result
The results show that rat local skin indigo plant contaminates after sensitization, after being smeared by test product, rat can be significantly reduced by test product
Indigo plant dye skin dye content, with the more significant difference (P of model control group<0.01, P<0.05) (4 are shown in Table)
The influence that 4 sample of table reacts P of Rats CA
(5) experimental result
Note：Blank control group compares, * * P<0.01；Compared with model control group, * P<0.05.
(6) conclusion (of pressure testing)
Embodiment 1, embodiment 2, embodiment 3 gained plant extracts A, B, C, which can effectively have, inhibits the passive allergy of skin.
The Ku812 cells that three, embodiments 1, embodiment 2,3 gained plant extracts A, B, C of embodiment induce PAMCI
The influence of IL-8, IL-6
(1) experimental principle IL-6 is a kind of multifunctional cytokine, and the mononuclear macrophage activated in blood is that it is main
Source.It is also to start the strongest cell factor of systemic inflammatory reaction, is capable of the increment differentiation of inducing T cell and B cell, thorn
Swash liver cell and generate acute phase protein etc., so that inflammatory effect is amplified by cascade reaction.Virus, bacterium infection can induce in vivo
IL-6 expression increases.
IL-8 is the cell factor secreted by Th1 cells, the immune response of main mediated cell poison and local inflammation-related
Auxiliary antibody generates, and participates in the generation of cellular immunity and the super quick type inflammation of delayed, and principal biological effect is chemotactic and activates
Neutrophil leucocyte promotes lysosomal enzyme activities and the phagocytosis of neutrophil leucocyte, also has one to basophilic granulocyte and T cell
Metamorphosis occurs after being contacted with IL-8 for fixed chemotaxis, neutrophil leucocyte, and orientation migration to reactive site simultaneously discharges a system
Row activated product, these effects can lead to body local inflammation reaction, achieve the purpose that sterilization and damaging cells.
Show that plant extracts has to the inhibition of inflammatory factor IL-6 and IL-8 to inhibit by ultraviolet injury, free radical oxygen
Scytitis caused by change, environmental pollution, hypersteatosis, bacterium infection etc., such as general red, itch.
(2) experimental method：Positive drug and embodiment 1, embodiment 2,3 gained plant of embodiment are prepared with DMSO or distilled water
Extract A, B, C, used time are diluted with containing 10% fetal calf serum culture medium, basic, normal, high a concentration of 25mg/mL, 50mg/mL,
100mg/mL。
Ku812 cell densities are adjusted to 2.2 × 10 with the IMDM culture mediums containing 10% fetal calf serum6The cell of a/mL
Suspension is inoculated in 96 orifice plates, per 200 μ L of hole.If cell blank control group, model group, positive drug group and test medicine group, often
Group sets 3 parallel holes, 37 DEG C, 5%CO2Incubator culture stands 10min, positive drug and test medicine group be added positive drug and by
Reagent object, final concentration of 50 μ g/mL set pre-temperature in incubator and incubate, and after 15min in addition to cell blank group, thorn is added in each group cell
Swash agent PMA, A12387 make final concentration be respectively 5nm, 1 μm, cell blank group be added the same volume culture mediums of DMSO containing isoconcentration.2h
Centrifugal separating cell afterwards collects cell supernatant, measures IL-8, IL-6 content in supernatant.
(3) the experimental result calculating basic, normal, high concentration of extract A is respectively to IL-6 inhibiting rates：10.3%, 16.9%,
30.7%；It is respectively to IL-8 inhibiting rates：6.7%, 18.3%, 31.6%；The basic, normal, high concentration of extract B is to IL-6 inhibiting rates
Respectively：7.8%, 15.5%, 28.3%；It is respectively to IL-8 inhibiting rates：8.1%, 17.4%, 29.9%；Extract C pair
IL-6 inhibiting rates are respectively：6.1%, 16.3%, 23.1%；It is respectively to IL-8 inhibiting rates：5.8%, 17.4%, 26.2%.
Show that embodiment 1, embodiment 2, embodiment 3 gained plant extracts A, B, C can inhibit I type super quick by inhibiting IL-6 to play
The effect for reacting (immediate hypersensitivity) inhibits IV type hypersensitivity (delayed allergy) by inhibiting IL-8 to play
Effect.
Four, embodiments 1, embodiment 2,3 gained plant extracts A, B, C of embodiment test hyaluronic acid enzyme inhibition
Method：
It is carried out using hyaluronidase body outer suppressioning experiment Elson-Morgan methods.Take 0.1mL 0.25mmol/L CaCl2
37 DEG C of heat insulating culture 20min of solution and 0.5mL hyaluronic acids enzyme solution；Plant extracts 0.5mL is added, continues 37 DEG C of heat insulating cultures
20min；0.5mL Sodium Hyaluronates liquid 37 DEG C of heat preservations 30min, room temperature 5min are added；0.1mL0.4mol/L NaOH are added
Solution and 0.5mL acetylacetone,2,4-pentanedione solution are placed in boiling water bath after heating 15min and carry out cooling 5min with ice water immediately；Addition angstrom
Er Lixi reagents 1.0mL is simultaneously diluted with 3.0mL absolute ethyl alcohols, 20min colour developings is placed, with its extinction of spectrophotometric determination
Angle value.
Antiallergic activity calculation formula：
In formula：A --- contrast solution ABS values (replace sample solution) with hac buffer
B --- control blank solution ABS values (replacing sample solution and enzyme solution with hac buffer)
C --- sample solution ABS values
D --- sample blank solution A BS values (replacing enzyme solution with hac buffer)
The length scanning of 450~700nm ranges is first carried out when experiment to A group samples, to determine maximum absorption wavelength, then
Using deionized water as reference, ABS value measurement is carried out respectively at the maximum absorption wavelength.
Each extract refers to table 5, experimental result to hyaluronic acid enzyme inhibition in vitro：
5 sample clear matter acid enzyme inhibition rate of table
Experiment conclusion：Embodiment 1, embodiment 2, embodiment 3 gained plant extracts A, B, C in vitro have hyaluronidase
Good inhibiting effect.
Five, embodiments 1, embodiment 2,3 gained plant extracts of embodiment inhibit surfactant effect of stimulation
Lauryl sodium sulfate is respectively adopted as surfactant stimulus in experiment, stimulates erythrocyte haemolysis.Selection
Reason：National standard GB17149.2-1997《Cosmetics contact dermatitis diagnostic criteria and treatment principle》In it is common in listed cosmetics
Sensibiligen, and data show that stimulation degree is larger in national standard, common cosmetic surfactants well known to those skilled in the art,
The mechanism of action of other anion surfactants is similar to lauryl sodium sulfate.The molten of erythrocyte is can be found that by experiment
Blood rate rises rapidly with the increase of surfactant concentration, and it is molten to will produce obvious red blood cell in 0.3% additive amount
Blood phenomenon.
By being previously added the embodiment 1 of various concentration into RBC Test System test systems, embodiment 2, implementing
3 gained plant extracts of example tests it to the irritating inhibiting effect of Typical surfactants lauryl sodium sulfate respectively.
The test of RBC haemolysis shows the plant extracts of embodiment 1, embodiment 2, embodiment 3 in 0.5% additive amount
Start to have and significantly inhibit effect of stimulation, inhibiting rate reaches 50% or so when 0.8%.With embodiment 1, embodiment 2,
3 plant extracts concentration of embodiment gradually increases, and the effect of stimulation of lauryl sodium sulfate is inhibited to gradually increase, when using dense
When degree is 1.2%, stimulation inhibition can reach 80% or so.Show the plant of embodiment 1, embodiment 2, embodiment 3
Extract has effects that good reduction surfactant is irritating.
Six, embodiments 1, embodiment 2,3 gained plant extracts of embodiment inhibit preservative effect of stimulation
According to national standard GB17149.2-1997《Cosmetics contact dermatitis diagnostic criteria and treatment principle》In listed makeup
Common sensibiligen in product, experiment as representing, measure it using Common Preservatives methyl p-hydroxybenzoate (methyl hydroxybenzoate)
Stimulate the power of erythrocyte haemolysis.Wherein, nipalgin lipid preservative is that the major class that those skilled in the art most often select is anti-
Rotten agent, methyl hydroxybenzoate can represent a major class preservative of the lipid containing nipalgin.The experimental results showed that the haemolysis of erythrocyte
Rate rises rapidly with the increase of concentration of preservatives, will produce obvious Haemolysis in 0.3% additive amount.
By being previously added the embodiment 1 of various concentration into RBC Test System test systems, embodiment 2, implementing
3 gained plant extracts of example tests it to the irritating inhibiting effect of methyl p-hydroxybenzoate respectively.
The test of RBC haemolysis shows the additive amount of embodiment 1, embodiment 2,3 gained plant extracts of embodiment 0.8%
When start to have and significantly inhibit effect of stimulation, inhibiting rate reaches 45% or so.With embodiment 1, embodiment 2, implement
3 gained plant extracts concentration of example gradually increases, it can be clearly seen that it inhibits stimulation effect caused by methyl p-hydroxybenzoate
Fruit gradually increases, and when using a concentration of 1.6%, inhibits effect of stimulation that can reach 75% or so.Show embodiment 1, implement
Example 2,3 gained plant extracts of embodiment have effects that good reduction preservative is irritating.
Seven, embodiments 1, embodiment 2,3 gained plant extracts of embodiment inhibit DNA Damage and leakage
DNA stores the hereditary information that organism depends on for existence and multiplies, therefore safeguards the integrality of DNA molecular to cell
And organism is most important.Factor inside external environment and organism all often leads to the damage or change of DNA molecular, such as
The damage of fruit DNA or the change of hereditary information cannot correct, and may influence function or the existence of cell.So during evolution
The ability for the DNA plerosis damage that biological cell is obtained just seems that particularly significant and biological energy source keeps the institute of genetic stability
.The adverse circumstances of nature, such as the irradiation of strong ultraviolet light, physical factor, chemical factor can cause the damage of DNA.
Inhibit DNA Damage and leakage can improve cutaneous immunisation power and resistance, with cope in adverse circumstances to radiation or other
The damage of stimulus.
Experimental method is ground with reference to screening, extraction and the mechanism of action of Wang Haitao antiallergic anti-irritant active materials used for cosmetic
Study carefully Beijing Technology and Business Universitys 2010:66.
Experiment is made using the embodiment 1, embodiment 2,3 gained plant extracts of embodiment of 1.0%, 5.0% two gradient
For test concentrations (diluting 20 times of uses when experiment), its protective effect to SDS induced DNA damages is measured respectively.
Pass through OxiSelectTMThe observation of Comet Assay kit, the damage that DNA is subject to is bigger, broken section of DNA just the more, more
Broken section of free speed of small DNA is just faster, and that also dissociates is remoter, and then forms the tail portion of comet, and some larger are broken
Fragment position forms the head of comet then close to nucleus.The degree difference of broken section of travelling of DNA makes image show comet
Shape.The length of comet and the being proportionate property of the extent of damage of DNA, comet tail end is longer, and the extent of damage of cell is bigger.
6 embodiment 1 of table, embodiment 2,3 gained plant extracts of embodiment inhibit DNA Damage comet result
Table 6
Comet picture is measured by image analysis software, analysis is found, in no embodiment 1, embodiment 2, real
In the presence of applying 3 gained plant extracts of example, 100% damage occurs for DNA, and the tail of a comet is about 45.5 ± 2.7 μm.It is adding in advance
Embodiment 1, embodiment 2, the protection of 3 gained plant extracts of embodiment, when then being stimulated with SDS, occur apparent DNA damage and subtract
The reduction of conspicuousness occurs for weak phenomenon, DNA damage cell hangover rate and tail of a comet length.Experimental result is shown in Table 6.
The experimental results showed that, embodiment 1, embodiment 2,3 gained plant extracts of embodiment to the damage of DNA and are let out above
Dew has good inhibiting effect.
Eight, embodiments 4-6 have effects that the cream that antiallergic is releived increases skin-tolerant clinical verification
Using arm inner surface smears the cream that embodiment 4-6 has effects that antiallergic releives on the skin in advance, 30 points
Pass through 10%SDS solution patch (areas in corresponding site after clock：2cm × 2cm) stimulation skin.Before experiment, by 60 volunteers
Respectively 3 groups, volunteer's forearm is cleaned up with clear water, forearm is divided into 3 pieces of regions, embodiment 4-6 is smeared in region 2 in advance to be had
Antiallergic is releived the cream of effect, and region 1 and region 3 are not processed.Sit quietly 30min, respectively in region 1 and the patch of region 2 10%
SDS patches, region 3 be used as complete blank control, it is without any processing.It is molten to SDS patches dropwise addition 0.2mLSDS per 15min
Liquid.After 45min, by skin coloration tester Mexameter MX18 test skin region erythema value E, reflection skin by
The degree of stimulation.
It is shown after experimental result statistics, 10%SDS stimulates rear region skin malaise symptoms apparent, occurs a series of as red
Swollen, itch condition susceptible.And smearing 4-6 containing embodiment in advance has effects that the cream that antiallergic is releived, then use 10%SDS
Stimulated zone malaise symptoms unobvious, corresponding red and swollen and itch phenomenon obviously weaken, and skin-tolerant is remarkably reinforced, and complete
White space compares, and the corresponding physical signs of skin is close；Comprehensive trizonal comparison can clearly show that embodiment 4-6 tools
There is the releive cream of effect of antiallergic that can enhance the tolerance of skin.
Nine, embodiments 4-6 and comparative example 1-7 have effects that the cream itching-relieving efficacies clinical verification that antiallergic is releived
Experimental method：Patient source is affiliated hospital of Zhongshan University Dermatology Outpatient Department patient.
This group totally 500, man 215, female 285, the oldest 45 years old, 21 years old minimum, course of disease longest 1 year was most 2 short
Month, it is divided into 10 groups at random.
Clinical manifestation has discomfort when being face, hand, lower limb, pedal skin, itches, furfur, after especially using cosmetics,
There is a series of symptom, repeatedly refractory (only for the crowd based on face of sensitive group, cosmetics can be with user；
Exclude severe allergy, systemic anaphylaxis and other patients with medical sign disease).
Methods of clinical observation and criterion of therapeutical effect
1, sample application method：
After daily face cleaning, applying embodiment 4-6 in face and sensitive part has effects that the cream that antiallergic is releived is appropriate, early
Evening is each primary.Respectively statistics is primary after 1 week, 4 weeks.
2, curative effect determinate standard
(1) it cures：Clinical symptoms disappear, condition susceptible subsides；
(2) effectively：Clinical symptom relief, condition susceptible subside or disappear, and reduce or reduce；
(3) invalid：Clinical symptoms, condition susceptible do not improve.
Clinical observation result and conclusion
1, result
Embodiment 4-6 and comparative example 1-7 has effects that the total situation of cream clinical observation and subject's disease that antiallergic is releived
The case where shape performance and curative effect, refers to table 7.
7 embodiment 4-6 of table has effects that the total situation of cream clinical observation that antiallergic is releived
2, clinical effectiveness is analyzed
(1) it is directed to skin of face sensitive group, skin of face cardinal symptom (itches, erythema, dry furfur situation, uses reality
Cream that a 4-6 has effects that antiallergic is releived is applied 1 week to efficient up to 90% (45), and 1 month cure rate is 72%
(36).
(2) it is learnt by embodiment 4-6 and comparative example 1-7 comparisons, it is Japan cypress, white willow, hundred golden flower, rheum rhabarbarum, Chuan Gu, big
When any of several broadleaf plants, rhodiola root only share, it is just notable to treat sensitive skin effect.
(3) trier has effects that Application Example 4-6 the overall assessment after cream that antiallergic is releived 4 weeks is shown in Table 8：
Table 8
(4) as long as adhering to that use is notable to sensitive skin crowd therapeutic effect.
Prepared by ten, embodiments 4-6 has effects that the cream white-skinned face function experiment that antiallergic is releived
1. experimental principle
Human experimentation forms test population by particular experiment crowd, and test subject uses cosmetics (and cosmetics work(
Imitate ingredient) before and after skin color variation, so that it is determined that the white-skinned face function of cosmetics (or functional component).
2. laboratory apparatus and sample
Instrument：Dermal melanin and ferroheme tester (Mexameter MX18, German CK electronics corporations production)；Lab
Color difference meter (MPA9, German CK electronics corporations production).
Sample：The cream that embodiment 4-6 is prepared.
3. Subject Population and testing time
Totally 150 people, age between 34~48 years old, are divided into 3 groups.
Volunteer's usage time：Sooner or later the cream for having effects that antiallergic and releiving smeared 1 embodiment 4-6 and prepared each daily
Frost.
Testing time：Test in one week is primary, tests 4 weeks.
4. experimental method
(1) before subject smears sample, first examination Test sites is cleaned, sample is smeared after drying.The left and right arm of subject
The tested region in inside and control zone, the area of each 4 × 4cm of determination sizes is as Test sites.
(2) subject takes appropriate cream painting to be uniformly put on test zone.Subject uses bare substrate in check plot simultaneously
Domain, it is sooner or later each daily to smear 1 time.During experiment, subject cannot smear any cosmetics in Test sites.
(3) subject's same time weekly after sample is used continuously, using dermal melanin and ferroheme tester with
Lab color difference meters test the variation of skin color, are averaged, are shown in Table 9：
Table 9
| Melanin content | Initial value | After 1 week | After 2 weeks | After 3 weeks | After 4 weeks |
| 4 control group of embodiment | 190.8 | 189.8 | 188.4 | 186.7 | 185.3 |
| Embodiment 4 | 190.8 | 183.3 | 177.1 | 170.5 | 164.3 |
| 5 control group of embodiment | 191.1 | 189.9 | 188.5 | 187.3 | 186.6 |
| Embodiment 5 | 191.1 | 184.5 | 177.3 | 170.4 | 165.2 |
| 6 control group of embodiment | 190.6 | 189.2 | 187.8 | 186.4 | 185.2 |
| Embodiment 6 | 190.6 | 183.2 | 176.4 | 169.3 | 164.7 |
5. interpretation of result
Melanin content mutation analysis
(1) as can be seen from Table 9, before using sample, sample sets are anisotropic with control group melanin content indifference；In sample
After use, more there is significant difference in each period sample sets compared with control group melanin content.
(2) sample sets using after 1 week compared with the control group compared with there is significantly otherness, melanin content is substantially reduced；
Sample sets inhibit melanin with obvious effects in 4 weeks, and duration is good.
6. conclusion
The above test results show that：
(1) after sampling, there is preferable inhibition melanin ability, and the lasting effect in 4 weeks is good.
(2) sampling first week increases the effect unobvious of skin brightness, tries out 2~4 weeks, and it is bright to increase skin
The significant effect of degree.Illustrate that this sample can effectively promote whitening effect.
The above described is only a preferred embodiment of the present invention, limitation in any form not is done to the present invention, therefore
All contents without departing from technical solution of the present invention, it is made to the above embodiment according to the technical essence of the invention any simply to repair
Change, equivalent variations and modification, in the range of still falling within technical solution of the present invention.
Claims (10)
1. a kind of skin care compositions for having effects that antiallergic and releiving, which is characterized in that prepared by the raw material of following weight parts proportioning
It forms：1-10 parts of Japan cypress, 1-10 parts of white willow, 1-10 parts of hundred golden flower, 1-15 parts of rheum rhabarbarum, paddy 1-10 parts of river, plantain 1-10
Part, 1-10 parts of rhodiola root.
2. the skin care compositions according to claim 1 for having effects that antiallergic and releiving, which is characterized in that by following weight parts
The raw material of proportioning is prepared：2-8 parts of Japan cypress, 2-8 parts of white willow, 2-8 parts of hundred golden flower, 2-10 parts of rheum rhabarbarum, paddy 2-8 parts of river,
2-8 parts of plantain, 2-8 parts of rhodiola root.
3. having effects that the preparation method for the skin care compositions that antiallergic is releived described in a kind of claims 1 or 2, which is characterized in that
The step of preparation method, is as follows：
(1) raw material described in claims 1 or 2 is weighed after crushing by the weight, mixing；
(2) it is the extraction of 70-90% ethyl alcohol with percent by volume, raw material is 1 with ethyl alcohol mass volume ratio g/mL：（10-30）, 70-
80 DEG C of extraction 1-4h；
(3) extracting solution that step (2) obtains is cooled to room temperature (20-30 DEG C), the filtering of 80-100 mesh obtains filtrate；
(4) 1,3-BDO that original amount of alcohol is added in filtrate is miscible uniform, evaporates the ethyl alcohol in solution, is filtered by vacuum,
Collect filtrate to get.
4. preparation method according to claim 3, which is characterized in that the percent by volume of ethyl alcohol is in the step (2)
80%, the mass volume ratio g/mL of raw material and ethyl alcohol is 1：20.
5. preparation method according to claim 3, which is characterized in that vacuum filtration condition is in cloth in the step (4)
1.2 μm of filter plates are spread in family name's funnel, are filtered by vacuum.
6. a kind of plant extracts for having effects that antiallergic and releiving, which is characterized in that the plant extracts is by claim 3-5
Any one of the preparation method be prepared.
7. plant extracts described in claim 6 is preparing the external use skin care additive or skin care system for having effects that antiallergic is releived
Purposes in agent.
8. a kind of skin care formulation for having effects that antiallergic and releiving, which is characterized in that the skin care formulation is planted described in claim 6
Object extract and skin care field customary adjuvant are made.
9. a kind of cream for having effects that antiallergic and releiving, which is characterized in that the cream includes the component of following weight percentage：
Plant extracts 1-10% described in claim 6
Cetostearyl alcohol 1-3%
- 2 0.5-2% of stereth
Ring five dimethyl silicone polymer 1-3%
Cyclohexasiloxane 0.1-1.5%
The poly- trimethicone 0.1-2% of diphenyl silicon phenyl
Butyrospermum parkii（BUTYROSPERMUM PARKII）Fruit fat 0.1-1%
Hydrogenated coco 0.1-2%
Dimethyl silicone polymer 1-2%
Saualane 0.1-2%
Olive（OLEA EUROPAEA）Fruit oil 0.1-2%
Tocopherol（Vitamin E） 0.1-1%
Hydrolecithin 0.1-1%
Methyl stearyl sodium taurocholate 0.1-1 %
Pentaerythrite four（Double-tert-butyl hydroxy hydrocinnamic acid）Ester 0.1-1%
Rice（ORYZA SATIVA）Oil extracted from rice husks 0.1-1%
Glycine betaine 0.5-2.5%
Tribehenin essence PEG-20 esters 0.1-1%
PEG-40 rilanit specials 0.01-0.1%
EDTA disodiums 0.01-0.05%
Sodium Hyaluronate 0.01-0.05%
Nephrite powder 0.01-0.2%
Xanthans 0.1-0.3%
Polyalcohol 5-15%
Preservative 0.05-0.2%
Water surplus.
10. the cream according to claim 9 for having effects that antiallergic and releiving, which is characterized in that the polyalcohol is sorb
Alcohol, D-sorbite, pungent glycol, dipropylene glycol, methyl-prop inositol, erythritol, maltitol, mannitol, pentanediol, ethyl
One or more kinds of mixing in hexyl glycerine, isoprene, hexylene glycol；The preservative is potassium sorbate, benzene first
At least one of alcohol, Phenoxyethanol.
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