CN108529017A - High concentration long-time cyropreservation transport device under a kind of room temperature - Google Patents
High concentration long-time cyropreservation transport device under a kind of room temperature Download PDFInfo
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- CN108529017A CN108529017A CN201810537127.5A CN201810537127A CN108529017A CN 108529017 A CN108529017 A CN 108529017A CN 201810537127 A CN201810537127 A CN 201810537127A CN 108529017 A CN108529017 A CN 108529017A
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- inner casing
- shell
- high concentration
- cyropreservation
- room temperature
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- 239000007788 liquid Substances 0.000 claims abstract description 35
- 239000012528 membrane Substances 0.000 claims abstract description 29
- 230000000149 penetrating effect Effects 0.000 claims abstract description 11
- 230000035699 permeability Effects 0.000 claims abstract description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 26
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 13
- 239000001569 carbon dioxide Substances 0.000 claims description 13
- 210000004027 cell Anatomy 0.000 description 66
- 230000032258 transport Effects 0.000 description 39
- 230000004083 survival effect Effects 0.000 description 10
- 238000010586 diagram Methods 0.000 description 6
- 230000008595 infiltration Effects 0.000 description 6
- 238000001764 infiltration Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003537 structural cell Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
- B65D81/22—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient in moist conditions or immersed in liquids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/24—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to high concentration long-time cyropreservation transport devices under a kind of room temperature, including the first shell, the first inner casing is equipped in the inner cavity of first shell, the side wall of first inner casing is equipped with the permeability hole for penetrating shell side wall in first, and the permeable membrane being connect with first inner casing is equipped at the permeability hole.High concentration long-time cyropreservation transport device under the room temperature of the present invention is adapted to transport high concentration cell liquid over long distances, for a long time and cell liquid is not easy to be contaminated by the setting of the first shell and the first inner casing.
Description
Technical field
The present invention relates to a kind of cell transportation devices more particularly to one kind transporting preservation in the long period at normal temperatures
The preservation transport device of high concentration cell.
Background technology
The method for being presently used for preserving and transporting fresh cells at normal temperatures is that cell and culture solution are sealed in a culture
In ware.In order to ensure to provide enough nutritional supports to cell in transportational process, while in order to ensure that cell metabolism is produced
Object can effectively be diluted, cell usually with lower concentration distribution in the medium, such as 1 × 105/ml.It transports in this way
Defeated cell needs centrifugal concentrating to be re-prepared as suitable concentration after arriving at, or needs to continue to cultivate several
It.The former increases process, while cell is easily contaminated, and the processing mode of the latter makes laboratory and clinical manipulation people
Member cannot use cell at once.The two, which is required for receiving unit, must have certain laboratory equipment.Therefore it is unfavorable for cell
The clinical for the treatment of product directly uses.On the other hand, culture solution oscillation causes cell and culture dish in transportational process in order to prevent
Wall, which generates, to be hit and leads to cell death, while leading to protein inactivation in order to avoid generating air bubble in culture solution, general meeting
Culture solution is filled in culture dish, excludes residual air.But such way cannot ensure cell in long-time transportational process
In continue to obtain air, high concentration carbon dioxide especially therein.Further, since culture solution is in direct contact with culture dish mouth,
If having in operation any careless, external bacterium is possible by culture solution micro in sealing-plug and enters test tube
It is interior, to generate pollution to invisible spectro cell.
Due to above-mentioned many reasons, after long-distance transportation, the survival rate of cell it is often relatively low (generally 5 0%~
60% or so) requirement used, cannot be met.And if it is transporting over long distances, for a long time, cell even can be because of survival rate too
It is low and can not recover.
It is obvious that at present in the solution to the several critical issues of cell means of transportation, in cell concentration, cell culture
Liquid, cannot take into account on the Railway Projects such as rich carbonated air (such as it is above-mentioned in order to ensure that cell was transporting
Survival rate in journey, the sufficient culture solution that needs in test tube to prestore is cells with nutrients.However, the addition of culture solution then reduces
The concentration of cell.After reaching transport destination, operating personnel also need to use after concentrating cell liquid, not only increase
Workload is added, has also increased the possibility of outside contamination).These limitations have seriously affected fresh cells and have grown at room temperature
Distance, prolonged transport.Therefore in the case where carrying out clinical cytology treatment on a large scale, current cell transportation resources is badly in need of
Obtain revolutionary change.
In view of the above shortcomings, the designer, is actively subject to research and innovation, to found a kind of new structural cell
Storage device makes it with more the utility value in industry.
Invention content
In order to solve the above technical problems, the object of the present invention is to provide one kind being suitable for long range, for a long time transport high concentration
Cell liquid and cell liquid are not easy high concentration long-time cyropreservation transport device under contaminated room temperature.
High concentration long-time cyropreservation transport device under the room temperature of the present invention, including the first shell, first shell
Inner cavity in be equipped with the first inner casing, the side wall of first inner casing is equipped with the permeability hole for penetrating shell side wall in first, described to ooze
Through-hole position is equipped with the permeable membrane being connect with first inner casing.
According to the above aspect of the present invention, the present invention has at least the following advantages：High concentration long-time cell is protected under the room temperature of the present invention
Transport device, including the first shell and the first inner casing are hidden, permeable membrane is provided on the first inner casing, when specifically used, operating personnel
Cell liquid is placed in the first inner casing, culture solution is placed in the first shell.The setting of permeable membrane so that be located in the first shell
Culture solution in chamber can be entered in the first inner casing by permeable membrane, to be the cells with nutrients in the first inner casing, this
Sample, operating personnel can store the higher cell liquid of concentration, while the setting of external medium in the first inner casing so that highly concentrated
The cell liquid of degree will not reduce its survival rate due to long-time transport, and cell liquid can directly make after transporting predeterminated target
With without being concentrated.Further, since cell liquid is stored in the first inner casing, it is not directly arranged in culture solution, cell
It is separately positioned with culture solution, to reduce its contaminated risk.
In conclusion high concentration long-time cyropreservation transport device is suitable for over long distances, for a long time under the room temperature of the present invention
It transports high concentration cell liquid and cell liquid is not easy to be contaminated.
Further, high concentration long-time cyropreservation transport device under room temperature of the invention, the top of first shell
End is equipped with the head cover with the first cage connection, and the top of first inner casing is set on the head cover.
Further, high concentration long-time cyropreservation transport device under room temperature of the invention, first shell, first
Inner casing is connect by screw thread with the head cover.
Further, high concentration long-time cyropreservation transport device, the head cover include upper cover under room temperature of the invention
Body, the lower cover below upper cover body and the first ventilated membrane between upper cover body, lower cover, the upper cover body and lower cover
The venthole being connected to the inner cavity of the inner cavity of first shell or the first inner casing is accordingly equipped on body.(venthole be in order to
Air or carbon dioxide enter cell liquid or culture solution, and the carbon dioxide and air being otherwise arranged in upper shell cannot propagate into
Into lower housing, upper shell just loses effect)
The setting of venthole so that outside air can be entered by venthole and ventilated membrane in the first shell, to
Air is provided for the culture solution in the first shell, to increase the air content in culture solution, further increases cell
Survival rate.
Further, high concentration long-time cyropreservation transport device under room temperature of the invention, first shell it is upper
Side is equipped with second housing.
Further, high concentration long-time cyropreservation transport device under room temperature of the invention, the second housing are interior also
Equipped with the second inner casing, the second housing, the second inner casing are connect with head cover, and the side wall of second inner casing, which is equipped with, penetrates the
The air hole of shell side wall in two, the second ventilated membrane being connect with the second inner casing is equipped at the air hole, and the venthole is located at
The middle part of head cover, and venthole is connected to the inner cavity of the inner cavity of the second inner casing, the first inner casing respectively.
When due to long distance transportation, high concentration long-time cyropreservation transport device is set to more simultaneously under multiple room temperature
In closed space, in the holding tank in box body, therefore its air content is less.For this purpose, the setting of second housing so that behaviour
A certain amount of air can be stored in second housing in advance by making personnel, to increase the survival rate of cell, be cell liquid it is long when
Between store and transport provide possibility.And the setting of the second inner casing so that the gas in the first inner casing can pass through the second inner casing
On the second ventilated membrane, venthole and the first ventilated membrane enter in the first inner casing, to further completely cut off extraneous air and the
The inner cavity of one inner casing, to avoid cell liquid contaminated.
Further, high concentration long-time cyropreservation transport device under room temperature of the invention, first shell and
It is both provided with rubber stopper on the side wall of two shells.
The setting of rubber stopper so that operating personnel can be injected into the first shell culture solution by rubber stopper or to outside second
Injected gas in shell, while culture solution or gas are not easy to be contaminated.
Further, high concentration long-time cyropreservation transport device under room temperature of the invention is set in the second housing
It is equipped with mixed gas, the mixed gas includes air and carbon dioxide, and the volume ratio of the carbon dioxide and air is 5%~
10%.
Carbon dioxide and air stoichiometry are most suitable for the growth of cell at 5%.Carbon dioxide can be by being arranged outside second
Sodium bicarbonate in shell is formed.
Further, high concentration long-time cyropreservation transport device under room temperature of the invention, first inner casing and
The volume ratio of one shell is 1:10~1:50, the volume ratio of the first inner casing and second housing is 1:10~1:50.
Further, high concentration long-time cyropreservation transport device under room temperature of the invention is set in first inner casing
It is equipped with cell liquid, culture solution is provided in first shell.
Above description is only the general introduction of technical solution of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, below with presently preferred embodiments of the present invention and after coordinating attached drawing to be described in detail such as.
Description of the drawings
Fig. 1 be embodiment one room temperature under high concentration long-time cyropreservation transport device structural schematic diagram；
Fig. 2 be embodiment two room temperature under high concentration long-time cyropreservation transport device structural schematic diagram；
Fig. 3 be embodiment two room temperature under high concentration long-time cyropreservation transport device structural schematic diagram；
Fig. 4 be example IV room temperature under high concentration long-time cyropreservation transport device structural schematic diagram；
Fig. 5 be embodiment five room temperature under high concentration long-time cyropreservation transport device structural schematic diagram；
Fig. 6 be embodiment six room temperature under high concentration long-time cyropreservation transport device structural schematic diagram；
Fig. 7 is cell survival rate curve graph corresponding with a kind of table 1 of embodiment.
In figure, 1：First shell；2：First inner casing；3：Permeable membrane；4：Head cover；5：Upper cover body；6：Lower cover；7：First thoroughly
Air film；8：Venthole；9：Rubber stopper；10：Cell liquid；11：Culture solution； 12：Second housing；13：Second inner casing；14：Second thoroughly
Air film.
Specific implementation mode
With reference to the accompanying drawings and examples, the specific implementation mode of the present invention is described in further detail.Implement below
Example is not limited to the scope of the present invention for illustrating the present invention.
Embodiment one：
Referring to Fig. 1,7, high concentration long-time cyropreservation transport device under the room temperature of the present embodiment, including the first shell 1,
The first inner casing 2 is equipped in the inner cavity of first shell, the side wall of the first inner casing is equipped with the permeability hole for penetrating shell side wall in first, oozes
Through-hole position is equipped with the permeable membrane 3 being connect with the first inner casing.
Preferably, the top of the first shell is equipped with the head cover 4 with the first cage connection, the top of the first inner casing is set to top
It covers.
Preferably, the first shell, the first inner casing are connect by screw thread with head cover.
Preferably, head cover include upper cover body 5, the lower cover 6 below upper cover body and positioned at upper cover body, lower cover it
Between the first ventilated membrane 7, the venthole 8 being connected to the inner cavity of the first shell is accordingly equipped in upper cover body and lower cover.
Preferably, being provided with rubber stopper 9 on the side wall of the first shell.
Preferably, being provided with cell liquid 10 in the first inner casing, culture solution 11 is provided in the first shell.
When specifically used, cell liquid is placed in the first inner casing by operating personnel, and culture solution is placed in the first shell.Infiltration
The setting of film so that the culture solution in the first shell inner cavity can be entered by permeable membrane in the first inner casing, to for
Cells with nutrients in first inner casing, in this way, operating personnel can store the higher cell liquid of concentration in the first inner casing, together
When culture solution setting so that the cell liquid of high concentration will not due to long-time transport and reduce its survival rate, cell liquid exists
It can be used directly after transporting predeterminated target without being concentrated.Further, since cell liquid is stored in the first inner casing, no
It is directly arranged in culture solution, cell is separately positioned with culture solution, to reduce its contaminated risk.
Following table 1 is that 1ml initial concentrations are 4 × 107The NAML-6 of/ml individually trains cell liquid using conventional method
It supports behind several days and is filled the case where cell viability and using high concentration long-time cyropreservation transport under the room temperature of the present embodiment
Set and place it in the culture solution of 25ml cultivate several days after cell viability the case where compare.The result shows that placing one week
Afterwards, the cell of the high concentration preserved using conventional method has already decreased to half, and the cell for using this method to preserve still surpasses
Cross 80% survival rate.
Table 1：4X107Survival rate compares at 25 DEG C of cells/ml
Embodiment two：
Referring to Fig. 2, high concentration long-time cyropreservation transport device under the room temperature of the present embodiment, including the first shell 1,
The first inner casing 2 is equipped in the inner cavity of one shell, the side wall of the first inner casing is equipped with the permeability hole for penetrating shell side wall in first, infiltration
The permeable membrane 3 being connect with the first inner casing is equipped at hole.
Preferably, the top of the first shell is equipped with the head cover 4 with the first cage connection, the top of the first inner casing is set to top
It covers.
Preferably, the first shell, the first inner casing are connect by screw thread with head cover.
Preferably, head cover include upper cover body 5, the lower cover 6 below upper cover body and positioned at upper cover body, lower cover it
Between the first ventilated membrane 7, the venthole 8 being connected to the inner cavity of the first inner casing is accordingly equipped in upper cover body and lower cover.
Preferably, being provided with rubber stopper 9 on the side wall of the first shell.
Preferably, being provided with cell liquid 10 in the first inner casing, culture solution 11 is provided in the first shell.
The present embodiment venthole is located at the middle part of head cover, has been connected to the inner cavity of air and the first inner casing so that cell liquid
Air can be directly absorbed from air.
Embodiment three：
Referring to Fig. 3, high concentration long-time cyropreservation transport device under the room temperature of the present embodiment, including the first shell 1,
The first inner casing 2 is equipped in the inner cavity of one shell, the side wall of the first inner casing is equipped with the permeability hole for penetrating shell side wall in first, infiltration
The permeable membrane 3 being connect with the first inner casing is equipped at hole.
Preferably, the top of the first shell is equipped with the head cover 4 with the first cage connection, the top of the first inner casing is set to top
It covers.
Preferably, the first shell, the first inner casing are connect by screw thread with head cover.
Preferably, head cover include upper cover body 5, the lower cover 6 below upper cover body and positioned at upper cover body, lower cover it
Between the first ventilated membrane 7, the venthole 8 being connected to the inner cavity of the first shell is accordingly equipped in upper cover body and lower cover.
The top of first shell is equipped with second housing 12.
Preferably, being both provided with rubber stopper on the side wall of the first shell, second housing.
Preferably, being provided with cell liquid in the first inner casing, culture solution is provided in the first shell.
Embodiment adds second housings, and when due to long distance transportation, high concentration long-time cell is protected under multiple room temperature
It hides transport device to be set in more closed space simultaneously, in the holding tank in box body, therefore its air content is less.For
This, the setting of second housing so that operating personnel can store a certain amount of air in second housing in advance, to increase cell
Survival rate, for cell liquid long-time storage and transport provides possibility.
Example IV：
Referring to Fig. 4, high concentration long-time cyropreservation transport device under the room temperature of the present embodiment, including the first shell 1,
The first inner casing 2 is equipped in the inner cavity of one shell, the side wall of the first inner casing is equipped with the permeability hole for penetrating shell side wall in first, infiltration
The permeable membrane 3 being connect with the first inner casing is equipped at hole.
Preferably, the top of the first shell is equipped with the head cover 4 with the first cage connection, the top of the first inner casing is set to top
It covers.
Preferably, the first shell, the first inner casing are connect by screw thread with head cover.
Preferably, head cover include upper cover body 5, the lower cover 6 below upper cover body and positioned at upper cover body, lower cover it
Between the first ventilated membrane 7, the venthole 8 being connected to the inner cavity of the first inner casing is accordingly equipped in upper cover body and lower cover.
The top of first shell is equipped with second housing 9.
Preferably, being both provided with rubber stopper on the side wall of the first shell and second housing.
Preferably, being provided with cell liquid in the first inner casing, culture solution is provided in the first shell.
Compared to embodiment three, the present embodiment venthole is located at the middle part of head cover, has been connected in the first shell and first
The inner cavity of shell so that cell liquid directly can absorb air from the first shell.
Embodiment five：
Referring to Fig. 5, high concentration long-time cyropreservation transport device under the room temperature of the present embodiment, including the first shell 1,
The first inner casing 2 is equipped in the inner cavity of one shell, the side wall of the first inner casing is equipped with the permeability hole for penetrating shell side wall in first, infiltration
The permeable membrane 3 being connect with the first inner casing is equipped at hole.
Preferably, the top of the first shell is equipped with the head cover 4 with the first cage connection, the top of the first inner casing is set to top
It covers.
Preferably, the first shell, the first inner casing are connect by screw thread with head cover.
Preferably, head cover include upper cover body 5, the lower cover 6 below upper cover body and positioned at upper cover body, lower cover it
Between the first ventilated membrane 7, the venthole 8 being connected to the inner cavity of the first shell is accordingly equipped in upper cover body and lower cover.
The top of first shell is equipped with second housing 9.
The second inner casing 13 is additionally provided in second housing, second housing, the second inner casing are connect with head cover, the side of the second inner casing
Wall is equipped with the air hole for penetrating shell side wall in second, is equipped with the second ventilated membrane 14 being connect with the second inner casing at air hole, leads to
Stomata is located at the middle part of head cover, and venthole is connected to the inner cavity of the inner cavity of the second inner casing, the first inner casing respectively.
Preferably, being both provided with rubber stopper on the side wall of the first shell and second housing.
Preferably, being provided with cell liquid in the first inner casing, culture solution is provided in the first shell.
Embodiment adds the second inner casing, the second inner casing makes cell liquid be less susceptible to by external contamination.
Preferably, being provided with mixed gas in second housing, mixed gas includes air and carbon dioxide, carbon dioxide
Volume ratio with air is 5%~10%.
Carbon dioxide and air stoichiometry are most suitable for the growth of cell at 5%.Carbon dioxide can be by being arranged outside second
Sodium bicarbonate in shell is formed.
Preferably, the volume ratio of the first inner casing and the first shell is 1:10~1:50, the first inner casing and second housing
Volume ratio is 1:10~1:50.
Embodiment six：
Referring to Fig. 6, high concentration long-time cyropreservation transport device under the room temperature of the present embodiment, including the first shell 1,
The first inner casing 2 is equipped in the inner cavity of one shell, the side wall of the first inner casing is equipped with the permeability hole for penetrating shell side wall in first, infiltration
The permeable membrane 3 being connect with the first inner casing is equipped at hole.
Preferably, the top of the first shell is equipped with the head cover 4 with the first cage connection, the top of the first inner casing is set to top
It covers.
Preferably, the first shell, the first inner casing are connect by screw thread with head cover.
Preferably, head cover include upper cover body 5, the lower cover 6 below upper cover body and positioned at upper cover body, lower cover it
Between the first ventilated membrane 7, the venthole 8 being connected to the inner cavity of the first shell is accordingly equipped in upper cover body and lower cover.
It is additionally provided with the second inner casing 13, the second inner casing is connect with head cover, and the side wall of the second inner casing, which is equipped with, penetrates the second inner casing
The air hole of side wall is equipped with the second ventilated membrane 14 being connect with the second inner casing at air hole, and venthole is located at the middle part of head cover, and
And venthole is connected to the inner cavity of the inner cavity of the second inner casing, the first inner casing respectively.
Preferably, being provided with rubber stopper on the first side wall of outer shell.
Preferably, being provided with cell liquid in the first inner casing, culture solution is provided in the first shell.
Compared to embodiment five, the present embodiment is not provided with second housing.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case of spirit or essential attributes of the invention, those skilled in the art can realize this in other specific forms
Invention.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, the present invention
Protection domain be indicated by the appended claims rather than the foregoing description.
In addition, the above is only a preferred embodiment of the present invention, being not intended to restrict the invention, it is noted that right
For those skilled in the art, without departing from the technical principles of the invention, it can also make several
Improvement and modification, these improvements and modifications also should be regarded as protection scope of the present invention.Although it will also be appreciated that this specification
Be described in terms of embodiments, every embodiment includes only a single technical solution, specification it is this
Narrating mode is merely for the sake of clarity, and the skilled in the art should refer to the specification as a whole, in each embodiment
Technical solution may also be suitably combined to form that other embodiments which can be understood by those skilled in the art.
Claims (10)
1. high concentration long-time cyropreservation transport device under a kind of room temperature, it is characterised in that：Including the first shell, described first
The first inner casing is equipped in the inner cavity of shell, the side wall of first inner casing is equipped with the permeability hole for penetrating shell side wall in first, institute
State the permeable membrane for being equipped at permeability hole and being connect with first inner casing.
2. high concentration long-time cyropreservation transport device under room temperature according to claim 1, it is characterised in that：Described
The top of one shell is equipped with the head cover with the first cage connection, and the top of first inner casing is set on the head cover.
3. high concentration long-time cyropreservation transport device under room temperature according to claim 2, it is characterised in that：Described
One shell, the first inner casing are connect by screw thread with the head cover.
4. high concentration long-time cyropreservation transport device under room temperature according to claim 3, it is characterised in that：The top
Lid includes upper cover body, the lower cover below upper cover body and the first ventilated membrane between upper cover body, lower cover, it is described on
The venthole being connected to the inner cavity of the inner cavity of first shell or the first inner casing is accordingly equipped on lid and lower cover.
5. high concentration long-time cyropreservation transport device under room temperature according to claim 4, it is characterised in that：Described
The top of one shell is equipped with second housing.
6. high concentration long-time cyropreservation transport device under room temperature according to claim 5, it is characterised in that：Described
The second inner casing is additionally provided in two shells, the second housing, the second inner casing are connect with head cover, on the side wall of second inner casing
Equipped with the air hole for penetrating shell side wall in second, the second ventilated membrane being connect with the second inner casing is equipped at the air hole, it is described
Venthole is located at the middle part of head cover, and venthole is connected to the inner cavity of the inner cavity of the second inner casing, the first inner casing respectively.
7. high concentration long-time cyropreservation transport device under room temperature according to claim 6, it is characterised in that：Described
It is both provided with rubber stopper on the side wall of one shell and second housing.
8. high concentration long-time cyropreservation transport device under room temperature according to claim 6, it is characterised in that：Described
Mixed gas is provided in two shells, the mixed gas includes air and carbon dioxide, the body of the carbon dioxide and air
Product is than being 5%~10%.
9. high concentration long-time cyropreservation transport device under room temperature according to claim 1, it is characterised in that：Described
The volume ratio of one inner casing and the first shell is 1:10~1:50, the volume ratio of the first inner casing and second housing is 1:10~1:50.
10. high concentration long-time cyropreservation transport device under room temperature according to claim 1, it is characterised in that：It is described
It is provided with cell liquid in first inner casing, culture solution is provided in first shell.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810537127.5A CN108529017A (en) | 2018-05-30 | 2018-05-30 | High concentration long-time cyropreservation transport device under a kind of room temperature |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810537127.5A CN108529017A (en) | 2018-05-30 | 2018-05-30 | High concentration long-time cyropreservation transport device under a kind of room temperature |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050101009A1 (en) * | 2003-11-10 | 2005-05-12 | Wilson John R. | Compartmentalized device for cell culture, cell processing, and sample dialysis |
| CN102015997A (en) * | 2008-05-05 | 2011-04-13 | 威尔森沃尔夫制造公司 | Cell container |
| CN102602605A (en) * | 2012-03-13 | 2012-07-25 | 张一心 | Novel sample storage bottle |
| US20140356939A1 (en) * | 2012-01-04 | 2014-12-04 | Japan Tissue Engineering Co., Ltd | Cell separation container |
| CN207109018U (en) * | 2017-08-22 | 2018-03-16 | 江苏健安生物科技有限公司 | A kind of modular cell culture and transport assembly |
| CN208560343U (en) * | 2018-05-30 | 2019-03-01 | 江苏健安生物科技有限公司 | High concentration long-time cyropreservation transport device under a kind of room temperature |
-
2018
- 2018-05-30 CN CN201810537127.5A patent/CN108529017A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050101009A1 (en) * | 2003-11-10 | 2005-05-12 | Wilson John R. | Compartmentalized device for cell culture, cell processing, and sample dialysis |
| CN102015997A (en) * | 2008-05-05 | 2011-04-13 | 威尔森沃尔夫制造公司 | Cell container |
| US20140356939A1 (en) * | 2012-01-04 | 2014-12-04 | Japan Tissue Engineering Co., Ltd | Cell separation container |
| CN102602605A (en) * | 2012-03-13 | 2012-07-25 | 张一心 | Novel sample storage bottle |
| CN207109018U (en) * | 2017-08-22 | 2018-03-16 | 江苏健安生物科技有限公司 | A kind of modular cell culture and transport assembly |
| CN208560343U (en) * | 2018-05-30 | 2019-03-01 | 江苏健安生物科技有限公司 | High concentration long-time cyropreservation transport device under a kind of room temperature |
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