CN108296213B - Plate washer - Google Patents
Plate washer Download PDFInfo
- Publication number
- CN108296213B CN108296213B CN201711431931.7A CN201711431931A CN108296213B CN 108296213 B CN108296213 B CN 108296213B CN 201711431931 A CN201711431931 A CN 201711431931A CN 108296213 B CN108296213 B CN 108296213B
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- China
- Prior art keywords
- liquid
- needle
- tray
- groove
- head
- Prior art date
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- 239000007788 liquid Substances 0.000 claims abstract description 511
- 238000002347 injection Methods 0.000 claims abstract description 125
- 239000007924 injection Substances 0.000 claims abstract description 125
- 230000007246 mechanism Effects 0.000 claims abstract description 88
- 239000011148 porous material Substances 0.000 claims abstract description 7
- 238000007667 floating Methods 0.000 claims description 24
- 238000000926 separation method Methods 0.000 claims description 23
- 238000004891 communication Methods 0.000 claims description 17
- 230000005540 biological transmission Effects 0.000 claims description 15
- 238000012423 maintenance Methods 0.000 abstract description 7
- 238000005213 imbibition Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 238000004140 cleaning Methods 0.000 description 24
- 239000002699 waste material Substances 0.000 description 7
- 238000007789 sealing Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000002965 ELISA Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 3
- 230000000149 penetrating effect Effects 0.000 description 3
- 230000037452 priming Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B15/00—Preventing escape of dirt or fumes from the area where they are produced; Collecting or removing dirt or fumes from that area
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a plate washer, which comprises a liquid injection mechanism, a liquid suction mechanism and a tray mechanism, wherein the liquid injection mechanism comprises a liquid injection head, the liquid suction mechanism comprises a liquid suction head and a first driving mechanism, the liquid suction head and the liquid injection head are arranged side by side along the left-right direction, and the first driving mechanism is connected with the liquid suction head to drive the liquid suction head to move up and down; the tray mechanism comprises a tray and a second driving mechanism, the tray is suitable for loading the micro-pore plate, and the second driving mechanism is connected with the tray to drive the tray to rotate at the lower positions of the liquid suction head and the liquid injection head. The plate washer provided by the embodiment of the invention is provided with the independent liquid injection mechanism and the independent liquid suction mechanism, has a simple structure and is convenient to process. In addition, when needs clearance or maintenance, the liquid mechanism or the imbibition mechanism of annotating can be purposefully dismantled, because inside pipeline and passageway simple structure, its clearance or maintenance are more convenient.
Description
Technical Field
The invention relates to biochemical equipment, in particular to a plate washer.
Background
The plate washer is specially used for cleaning the ELISA plate and is generally matched with an ELISA plate reader. Has been widely used for the ELISA plate cleaning work of hospitals, blood stations, sanitary and epidemic prevention stations, reagent factories and research rooms.
The plate washer in the related art is generally composed of a cleaning liquid bottle, a peristaltic pump, a vacuum pump and a cleaning head, wherein the cleaning head is provided with a liquid injection needle and a liquid suction needle, cleaning liquid is firstly pumped out of the cleaning liquid bottle through the peristaltic pump, is discharged into micropores of the ELISA plate through the liquid injection needle on the cleaning head, is used for cleaning the micropores, and liquid in the micropores after cleaning is sucked into the liquid suction needle through the adsorption effect of the vacuum pump and is then sucked into the waste liquid bottle through the liquid suction head to be discharged. In the above-mentioned structure, annotate liquid needle and imbibition needle all set up on the cleaning head, and quantity is great, for example 8 needles, 12 needles, 32 needles, 48 needles, 96 needles etc. need to set up independent imbibition passageway and annotate liquid passageway on the cleaning head to, these imbibition needles all need with imbibition passageway intercommunication, annotate liquid needle all need with annotate liquid passageway intercommunication, consequently, its structural design is complicated, and the processing degree of difficulty is big. In addition, because the liquid sucking needle and the liquid injecting needle are easy to be blocked in the using process, the cleaning and maintenance are difficult.
Disclosure of Invention
The present invention aims to solve at least one of the technical problems in the related art to some extent. To this end, the invention aims to propose a plate washer.
To achieve the above object, in one aspect, a plate washer according to an embodiment of the present invention includes:
The liquid injection mechanism comprises a liquid injection head;
The liquid suction mechanism comprises a liquid suction head and a first driving mechanism, the liquid suction head and the liquid injection head are arranged side by side along the left-right direction, and the first driving mechanism is connected with the liquid suction head so as to drive the liquid suction head to move up and down;
The tray mechanism comprises a tray and a second driving mechanism, the tray is suitable for loading the micro-pore plates, and the second driving mechanism is connected with the tray to drive the tray to rotate at the lower positions of the liquid suction head and the liquid injection head.
According to the plate washer provided by the embodiment of the invention, the plate washer is provided with the independent liquid injection mechanism and the liquid suction mechanism, the tray mechanism is configured, the tray mechanism comprises the tray and the second driving mechanism, the tray is suitable for loading the micro-pore plate, the second driving mechanism can drive the tray to rotate at the position below the liquid suction head of the liquid suction mechanism and the liquid injection head of the liquid injection mechanism, and therefore the liquid injection operation and the liquid suction operation can be respectively completed by the liquid injection mechanism and the liquid suction mechanism. The liquid injection mechanism and the liquid suction mechanism which are independently arranged can simplify the structure and facilitate the processing. In addition, when needs clearance or maintenance, the liquid mechanism or the imbibition mechanism of annotating can be purposefully dismantled, because inside pipeline and passageway simple structure, its clearance or maintenance are more convenient.
In addition, the plate washer according to the above embodiment of the present invention may have the following additional technical features:
according to one embodiment of the invention, the first driving mechanism includes:
The support is provided with a screw rod and a first guide rod which extend in the up-down direction;
the floating plate is in threaded sleeve fit with the screw rod, and the floating plate is in sliding sleeve fit with the first guide rod;
The output shaft of the first motor extends along the up-down direction and is connected with the screw rod so as to drive the screw rod to rotate and enable the floating plate to move up and down;
the liquid suction head is connected with the floating plate so as to move up and down along with the floating plate.
According to one embodiment of the invention, the second driving mechanism includes:
the transmission assembly comprises a driving wheel, a driven wheel and a transmission belt, and the transmission belt is sleeved between the driving wheel and the driven wheel;
the clamping block is clamped on the transmission belt;
the guide assembly comprises a first support, a second guide rod connected between the first support and the second support and a sliding block sleeved on the second guide rod in a sliding manner, the second guide rod extends along the left-right direction, and the sliding block is fixedly connected with the clamping block;
the output shaft of the second motor extends along the front-back direction and is connected with the driving wheel so as to drive the driving wheel to rotate and enable the driving belt to drive the clamping block and the sliding block to move left and right;
The tray is fixedly connected with the sliding block so as to slide left and right along with the sliding block.
According to one embodiment of the invention, the priming head comprises:
The first needle seat is provided with a plurality of first liquid distribution tanks and at least one communication tank on the top surface, and the plurality of first liquid distribution tanks are communicated through at least one communication tank; the inside of the first needle seat is provided with a liquid injection channel, one end of the liquid injection channel is formed into a liquid inlet, the liquid inlet is positioned on the side surface of the needle seat, the other end of the liquid injection channel is formed into a first liquid outlet, and the first liquid outlet upwards penetrates through at least one communication groove;
the first cover plate is detachably arranged on the top surface of the first needle seat and seals the first liquid distributing groove and the communication groove;
The liquid needle is annotated to multirow, the multirow annotate the liquid needle with a plurality of first branch cistern one-to-one, every annotate the liquid needle wear to locate on the first needle file, just annotate the upper end of liquid needle with first branch cistern is linked together, annotate the lower extreme of liquid needle and wear out from the bottom surface of first needle file.
According to one embodiment of the invention, the upper end of each liquid injection needle is higher than the bottom surface of the first liquid separation groove.
According to one embodiment of the invention, the pipette head comprises:
the top surface of the second needle seat is provided with at least one liquid groove unit, each liquid groove unit comprises a main liquid groove and a plurality of second liquid grooves, the second liquid grooves are communicated with the main liquid groove, and the bottom surface of the main liquid groove is lower than the bottom surface of each second liquid groove;
the second cover plate is detachably arranged on the top surface of the second needle seat and seals the liquid tank unit;
at least one second liquid outlet is formed in the second needle seat or the second cover plate, the at least one second liquid outlet corresponds to the at least one liquid tank unit one by one, and each second liquid outlet is communicated with the corresponding main liquid tank;
At least one liquid sucking needle unit, wherein the at least one liquid sucking needle unit corresponds to at least one liquid groove unit one by one, each liquid sucking needle unit comprises a plurality of rows of liquid sucking needles, and the liquid sucking needles correspond to a plurality of second liquid separating grooves one by one; each liquid suction needle penetrates through the second needle seat, the upper end of each liquid suction needle is communicated with the second liquid separation groove, and the lower end of each liquid suction needle penetrates out of the bottom surface of the second needle seat.
According to one embodiment of the invention, the second liquid outlet is arranged on the side surface of the second needle seat and is lower than the bottom surface of the second liquid separating groove.
According to one embodiment of the invention, the upper end of each of the pipetting needles is higher than the bottom surface of the second pipetting channel.
According to one embodiment of the invention, a tray table is arranged below the tray, a sliding frame capable of sliding along the left-right direction is arranged on the tray table, a connecting arm extends out of one side of the sliding frame, the connecting arm is fixedly connected with the sliding block, and the tray is arranged on the sliding frame.
According to one embodiment of the invention, the tray table is provided with a liquid collecting tank, and the liquid collecting tank is positioned right below the liquid injection head.
Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of a plate washer according to an embodiment of the present invention;
FIG. 2 is a schematic view of the structure of the inside of the plate washer according to the embodiment of the present invention;
FIG. 3 is a schematic view of an exploded construction of an embodiment of the invention in a plate washer;
FIG. 4 is a schematic view of the structure of the liquid filling head in the plate washer according to the embodiment of the invention;
FIG. 5 is an exploded view of the injector head of the trigger of the present invention;
fig. 6 is a top view of the first hub of the injector head in the plate washer according to the embodiment of the present invention;
FIG. 7 is a cross-sectional view taken at A-A of FIG. 6;
FIG. 8 is a cross-sectional view taken at B-B in FIG. 6;
Fig. 9 is a schematic view of the structure of a liquid suction head in the plate washer according to the embodiment of the present invention;
fig. 10 is an exploded view of a pipette tip in a trigger of the present invention;
Fig. 11 is a cross-sectional view of a pipette tip in a plate washer according to an embodiment of the present invention;
fig. 12 is a cross-sectional view of the second hub of the pipette head in the plate washer of the embodiment of the present invention.
Reference numerals:
A liquid injection mechanism 100;
a liquid injection head 10;
A first needle holder 101;
a first liquid dividing tank 1011;
A communication groove 1012;
annular groove 1013;
A liquid injection passage 102;
A liquid inlet 1021;
A first liquid outlet 1022;
A first cover plate 103;
A liquid injection needle 104;
A first connector 105; a liquid suction mechanism 200;
A liquid suction head 20;
Second hub 201;
A liquid tank unit 2011;
a main liquid tank 2011a;
A second liquid separation tank 2011b;
A second cover plate 202;
A pipette needle unit 203;
a pipette needle 2031;
a second connector 204;
a first driving mechanism 21;
a bracket 211;
A screw 212;
A first guide bar 213;
A floating plate 214;
A first motor 215;
A tray mechanism 300;
a tray 30;
a second driving mechanism 31;
a driving wheel 311;
driven wheel 312;
a belt 313;
a clamping block 314;
A second guide bar 315;
A slider 316;
A second motor 317;
A first support 318;
A housing 400;
A fixing plate 401;
A pallet 402;
A liquid collection tank 4021;
A carriage 403;
Microplates W.
The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below are exemplary and intended to illustrate the present invention and should not be construed as limiting the invention, and all other embodiments, based on the embodiments of the present invention, which may be obtained by persons of ordinary skill in the art without inventive effort, are within the scope of the present invention.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "circumferential", "radial", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplify the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present invention, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
The following describes in detail a plate washer according to an embodiment of the present invention with reference to the accompanying drawings.
Referring to fig. 1 to 3, a plate washer according to an embodiment of the present invention includes a liquid injection mechanism 100, a liquid suction mechanism 200, and a tray mechanism 300.
Specifically, the liquid injection mechanism 100 includes the liquid injection head 10. It can be appreciated that the liquid injection mechanism 100 may further include a liquid injection pump, a liquid injection pipeline, and the like, wherein the liquid injection pump is connected with the liquid injection head 10 through the liquid injection pipeline, and is used for conveying liquid to the liquid injection head 10 through the liquid injection pipeline and finally injecting the liquid into the micropores on the microporous plate W through the liquid injection head 10.
The liquid suction mechanism 200 includes a liquid suction head 20 and a first driving mechanism 21, the liquid suction head 20 and the liquid injection head 10 are arranged side by side along the left-right direction, and the first driving mechanism 21 is connected with the liquid suction head 20 to drive the liquid suction head 20 to move up and down, that is, the first driving mechanism 21 can drive the liquid suction head 20 to move up and down. It can be appreciated that the liquid suction mechanism 200 may further include a liquid suction pump, a waste liquid bottle, a liquid suction pipeline, and the like, where the liquid suction pump is communicated with the waste liquid bottle through a conduit, the waste liquid bottle is connected with the liquid suction head 20 through the liquid suction pipeline, and negative pressure is generated through the liquid suction pump, so that the liquid suction head 20 sucks the liquid cleaned in the micropores of the microporous plate W into the liquid suction pipeline, and then enters the waste liquid bottle through the liquid suction pipeline.
The tray mechanism 300 includes a tray 30 and a second driving mechanism 31, wherein the tray 30 is suitable for loading the microplate W, and the second driving mechanism 31 is connected to the tray 30 to drive the tray 30 to rotate at a position below the pipetting head 20 and the pipetting head 10.
That is, the tray mechanism 300 is located below the pipetting head 10 and the pipetting head 20, the tray 30 is used to mount the microwell plate W, and the second driving mechanism 31 can drive the tray 30 to move in the left-right direction and can move to below the pipetting head 10 and below the pipetting head 20.
In a specific operation, a microplate W is mounted on the tray 30, and each of the microwells in the microplate W has liquid remaining after detection. First, the tray 30 is driven to move under the filling head 10 by the second driving mechanism 31, and then the filling head 10 fills the cleaning liquid into each of the wells on the microplate W. Then, the tray 30 is driven to move to the lower side of the liquid sucking head 20 by the second driving mechanism 31, and then the liquid sucking head 20 is driven to move downwards by the first driving mechanism 21, so that the liquid sucking head 20 is inserted into each micro-hole in the lower micro-hole plate W, and the liquid after cleaning in the micro-hole is sucked.
According to the plate washer provided by the embodiment of the invention, the plate washer is provided with the independent liquid injection mechanism 100 and the liquid suction mechanism 200, the tray mechanism 300 is configured, the tray mechanism 300 comprises the tray 30 and the second driving mechanism 31, the tray 30 is suitable for loading the micro-pore plate W, the second driving mechanism 31 can drive the tray 30 to rotate at the position below the liquid suction head 20 of the liquid suction mechanism 200 and the liquid injection head 10 of the liquid injection mechanism 100, and thus, the liquid injection operation and the liquid suction operation can be respectively finished by utilizing the liquid injection mechanism 100 and the liquid suction mechanism 200. The separate liquid injection mechanism 100 and the liquid suction mechanism 200 can simplify the structure and facilitate the processing. In addition, when cleaning or maintenance is needed, the liquid injection mechanism 100 or the liquid suction mechanism 200 can be disassembled in a targeted manner, and the cleaning or maintenance is more convenient due to the simple structure of the internal pipelines and the channels.
In addition, since the cleaning liquid is injected into the micro holes of the micro holes plate W and the cleaning liquid is relatively clean when the liquid injection head 10 injects the liquid, the problem of clogging inside the liquid injection head 10 is relatively small, and the liquid suction head 20 sucks the liquid in the micro holes of the micro holes W during the liquid suction, and the liquid in the micro holes contains residual sample, reagent, cleaning liquid, etc., so that the inside of the liquid suction head 20 is easily clogged due to the sample (e.g., blood), and therefore, the liquid suction head 20 is relatively frequently cleaned and maintained. In the invention, the liquid suction head 20 and the liquid injection head 10 are independently arranged, so that the liquid suction head and the liquid injection head are not mutually influenced, and the liquid suction head and the liquid injection head are convenient to clean and maintain in a targeted manner.
Referring to fig. 1 to 2, for example, the plate washer may further include a housing 400 and a fixing plate 401 disposed in the housing 400, the liquid filling head 10 is fixedly mounted on a front side surface of the housing 400, the first driving mechanism 21 and the second driving mechanism 31 are mounted on the fixing plate 401 in the housing, the liquid filling head 20 extends from the front side surface, the tray 30 is located outside the housing 400 and below the liquid filling head 20 and the liquid filling head 10, so that the structure is simpler and more compact, and the appearance is simpler.
In one embodiment of the present invention, the first driving mechanism 21 includes a bracket 211, a floating plate 214, and a first motor 215, wherein the bracket 211 is provided with a screw 212 and a first guide bar 213 extending in an up-down direction. The floating plate 214 is in threaded fit with the screw rod 212, and the floating plate 214 is in sliding fit with the first guide rod 213. An output shaft of the first motor 215 extends in the up-down direction and is connected to the screw 212 to drive the screw 212 to rotate so as to move the floating plate 214 up-down. The pipette head 20 is coupled to the floating plate 214 to move up and down with the floating plate 214.
That is, the floating plate 214 has a screw hole and a through hole, the screw rod 212 is screw-fitted with the screw hole, and the first guide rod 213 is slide-fitted with the through hole. When the first motor 215 is operated, the output shaft of the first motor 215 drives the screw rod 212 to rotate, and the screw rod 212 drives the floating plate 214 to move in the up-down direction due to the threaded engagement between the screw rod 212 and the floating plate 214, and at the same time, the floating plate 214 can be ensured to move in the up-down direction more smoothly and reliably due to the sliding engagement between the first guide rod 213 and the floating plate 214 by the guide action of the first guide rod 213. Since the liquid suction head 20 is connected to the floating plate 214 by fixing the same with a screw, the floating plate 214 can drive the liquid suction head 20 to move up and down when moving up and down, and the structure is simple and the up and down movement is smooth and reliable.
Referring to fig. 3, in one embodiment of the present invention, the second driving mechanism 31 includes a transmission assembly, a clamping block 314, a guiding assembly, and a second motor 317, where the transmission assembly includes a driving wheel 311, a driven wheel 312, and a driving belt 313, the driving belt 313 is sleeved between the driving wheel 311 and the driven wheel 312, and the clamping block 314 is clamped on the driving belt 313; the guiding assembly comprises a first support 318, a second support, a second guiding rod 315 connected between the first support 318 and the second support, and a sliding block 316 slidably sleeved on the second guiding rod 315, wherein the second guiding rod 315 extends along the left-right direction, and the sliding block 316 is fixedly connected with the clamping block 314; an output shaft of the second motor 317 extends in the front-rear direction and is connected to the driving wheel 311, so as to drive the driving wheel 311 to rotate, so that the driving belt 313 drives the clamping block 314 and the sliding block 316 to move left and right; the tray 30 is fixedly connected with the sliding block 316 so as to slide left and right along with the sliding block 316.
When the second motor 317 works, the output shaft of the second motor 317 drives the driving wheel 311 to rotate, the driving wheel 311 drives the driving belt 313 to rotate, and since the clamping block 314 is clamped and fixed on the driving belt 313, the driving belt 313 can drive the clamping block 314 to move in a left-right direction when rotating, for example, when the second motor 317 rotates in one of a forward direction and a reverse direction, the driving belt 313 rotates clockwise, at this time, the clamping block 314 moves rightward, when the second motor 317 rotates in the other of the forward direction and the reverse direction, the driving belt 313 rotates counterclockwise, at this time, the clamping block 314 moves leftward, and thus, the clamping block 314 can be driven to move left and right by the forward rotation and the reverse rotation of the second motor 317. Since the sliding block 316 is slidably disposed on the second guide rod 315 and the sliding block 316 is fixedly connected to the clamping block 314, the sliding block 316 can move left and right along with the clamping block 314, and can move left and right smoothly and reliably under the guidance of the second guide rod 315. Since the tray 30 is fixedly connected to the slider 316, the tray 30 can be driven to move in the left-right direction when the slider 316 moves in the left-right direction.
It should be noted that, in this embodiment, the transmission assembly composed of the driving wheel 311, the driven wheel 312 and the transmission belt 313 is adopted, the transmission is more stable, and the cleaning solution is in the micro-pore plate W on the tray 30, so in the motion process, if vibration or shake occurs, the cleaning solution splashes out, so in this embodiment, the transmission is ensured to be stable through the transmission assembly, so that the micro-pore plate W can not have the problems of cleaning solution splashes out in the moving process, and the clean and sanitary of the working area in the cleaning process is maintained. In addition, the second driving mechanism 31 of the above structure is simple in structure and convenient in assembly.
Referring to fig. 4 to 8, in one embodiment of the present invention, the priming head 10 comprises a first needle holder 101, a first cover plate 103, and a plurality of priming needles 104, wherein a top surface S101 of the first needle holder 101 is provided with a plurality of first liquid dividing grooves 1011 and at least one communicating groove 1012, and a plurality of the first liquid dividing grooves 1011 are communicated with each other through at least one of the communicating grooves 1012; the inside of first needle file 101 is equipped with annotates liquid passageway 102, annotate the one end of liquid passageway 102 and form into inlet 1021, inlet 1021 is located the side S102 of first needle file 101, annotate the other end of liquid passageway 102 and form first liquid outlet 1022, first liquid outlet 1022 upwards link up at least one the intercommunication groove 1012. That is, the liquid inlet 1021, the liquid injection passage 102, the first liquid outlet 1022, the communication groove 1012, and the first liquid dividing groove 1011 are sequentially communicated.
The first cover plate 103 is detachably arranged on the top surface S101 of the first needle seat 101 and seals the first liquid distributing groove 1011 and the communicating groove 1012; that is, the first cover 103 is detachably connected to the first needle holder 101, and the first cover 103 seals the first liquid dividing groove 1011 and the communication groove 1012 after the first cover 103 is connected to the first needle holder 101.
The multiple rows of the liquid injection needles 104 are in one-to-one correspondence with the multiple first liquid separating tanks 1011, each liquid injection needle 104 is arranged on the first needle seat 101 in a penetrating manner, the upper ends of the liquid injection needles 104 are communicated with the first liquid separating tanks 1011, and the lower ends of the liquid injection needles 104 penetrate out from the bottom surface of the first needle seat 101. That is, each of the first liquid dividing tanks 1011 is correspondingly connected with a plurality of liquid injection needles 104, and the plurality of liquid injection needles 104 are sequentially arranged at intervals along the extending direction of the first liquid dividing tank 1011, and the liquid in the first liquid dividing tank 1011 can be discharged through the liquid injection needles 104.
In a specific application, the liquid inlet 1021 may be connected to a liquid injection pump through a liquid injection pipeline, and the liquid delivered by the liquid injection pump enters the liquid injection channel 102 from the liquid inlet 1021, then enters the communicating groove 1012 through the liquid outlet, then flows into each first liquid dividing groove 1011 through the communicating groove 1012, then flows into the liquid injection needle 104 from the first liquid dividing groove 1011, and finally is injected into each micro-hole on the micro-hole plate W through the liquid injection needle 104.
The number of the first liquid dividing grooves 1011 and the number of the liquid injection needles 104 may be configured according to specific needs, for example, may be configured according to the specification of the microplate W, in the example of fig. 2, two first liquid dividing grooves 1011 are provided, one communicating groove 1012 is provided, two first liquid dividing grooves 1011 are communicated through the communicating groove 1012, and 8 liquid injection needles 104 are provided in each first liquid dividing groove 1011, so that the requirement of injecting liquid into the microplate W according to the specification of 8×m (M is a natural number greater than 1) may be satisfied.
In the related art, the liquid injection head is generally of an integrated structure, that is, a liquid injection channel is directly formed in the side face of a plate body along the horizontal direction, a pinhole is formed in the bottom of the plate body along the vertical direction, and a liquid injection needle is arranged in the pinhole. In addition, the liquid injection speed of each liquid injection port is inconsistent, the liquid injection speed of the liquid injection needle close to the liquid injection port is high, and the liquid injection speed of the liquid injection needle far away from the liquid injection port is low. In addition, the liquid injection needle has the problem of hanging liquid after stopping liquid injection.
In this embodiment, this annotate liquid head 10 adopts split type structure, when the assembly, can clear up the piece in the first needle file 101 earlier, guarantees that there is not piece, burr etc. in the first needle file 101 afterwards, again assembles first apron 103 and first needle file 101 assembly, so, can avoid the problem such as notes liquid needle 104 jam that debris, burr etc. easily cause. In addition, after the filling needle 104 is blocked or used for a certain period of time, the first cover 103 may be opened to clean and dredge the first liquid separating groove 1011, the communicating groove 1012, dirt in the filling needle 104, and the like on the first needle holder 101.
Further, the first needle seat 101 is provided with the liquid injection channel 102, one end of the liquid injection channel 102 is formed into the liquid inlet 1021, the liquid inlet 1021 is located at the side surface S102 of the needle seat, the other end of the liquid injection channel 102 is formed into the first liquid outlet 1022, and the first liquid outlet 1022 penetrates the communication groove 1012 upwards, so that when liquid is injected, the liquid flows into the communication groove 1012 upwards through the first liquid outlet 1022 of the liquid injection channel 102, flows into each first liquid separating groove 1011 through the communication groove 1012, finally flows into the liquid injection needle 104 from each first liquid separating groove 1011, and because the liquid flows upwards from the first liquid outlet 1022, the liquid can only enter each liquid injection needle 104 after the liquid level rises wholly, and the problems that the liquid injection speed of the liquid injection needle close to the liquid injection port in the related art is high, the liquid injection speed of the liquid injection needle far away from the liquid injection port is slow and the like are avoided, and the consistency of the liquid injection speed of each liquid injection needle 104 is ensured.
Further, when the liquid injection is stopped, part of the liquid in the first liquid dividing groove 1011 and the communicating groove 1012 flows back to the liquid injection channel 102 through the first liquid outlet 1022, so that the liquid in the first liquid dividing groove 1011 and the communicating groove 1012 can be reduced, the liquid entering the liquid injection needle 104 can be reduced, and the problem of liquid hanging can be reduced to a certain extent.
In one embodiment of the present invention, the device further comprises a first connector 105, and the first connector 105 is disposed on the first needle stand 101 and is connected with the liquid inlet 1021 in a sealing manner.
That is, a first connector 105 is connected to the inlet 1021, so that the injection line can be connected to the injection pump through the first connector 105, and the injection line can be connected to the injection pump conveniently.
Referring to fig. 8, in some embodiments of the present invention, the upper end of each of the injection needles 104 is higher than the bottom surface S103 of the first liquid dividing groove 1011. On the other hand, if dirt is accumulated in the first liquid dividing groove 1011, the dirt is generally located on the bottom surface S103 of the first liquid dividing groove 1011, and therefore, the dirt is not likely to enter the injection needle 104, and the injection needle 104 is not likely to be clogged. On the other hand, when the liquid is injected, the first liquid outlet 1022 flows out to the communication groove 1012 and the first liquid dividing groove 1011, and the liquid can enter the injection needles 104 at the same time only when the liquid level rises above the upper ends of the injection needles 104, whereby the consistency of the injection speed of the injection needles 104 can be further improved.
As shown in fig. 5 to 8, advantageously, in one example of the present invention, the top surface S101 of the first needle holder 101 is provided with an annular groove 1013, the annular groove 1013 is provided around the first liquid dividing groove 1011 and the communicating groove 1012, and an annular seal ring is provided in the annular groove 1013 to seal the gap between the first cover plate 103 and the first needle holder 101, that is, a seal ring is provided between the first cover plate 103 and the top surface of the first needle holder 101, with which the gap between the first cover plate 103 and the first needle holder 101 can be sealed, so that a better sealing effect can be achieved.
Illustratively, the first cover 103 is connected to the first hub 101 by a plurality of screws, so that the first cover 103 can be conveniently connected to and detached from the first hub 101, and meanwhile, the structure is firm.
Referring to fig. 9 to 12, in one embodiment of the present invention, the liquid suction head 20 includes a second needle holder 201, a second cover plate 202 and at least one liquid suction needle unit 203, wherein at least one liquid groove unit 2011 is disposed on a top surface of the second needle holder 201, each liquid groove unit 2011 includes a main liquid groove 2011a and a plurality of second liquid separation grooves 2011b, each of the plurality of second liquid separation grooves 2011b is in communication with the main liquid groove 2011a, and a bottom surface S201 of the main liquid groove 2011a is lower than a bottom surface S202 of each of the second liquid separation grooves 2011 b.
Second cover plate 202 is detachably disposed on the top surface of second needle holder 201 and closes liquid tank unit 2011. That is, second cover plate 202 is detachably connected to second hub 201, and when second cover plate 202 is connected to second hub 201, second cover plate 202 may seal liquid tank unit 2011 such that main liquid tank 2011a and second liquid-dividing tank 2011b in liquid tank unit 2011 form a sealed state.
Second needle stand 201 or second cover plate 202 is provided with at least one second liquid outlet, at least one second liquid outlet corresponds to at least one liquid tank unit 2011 one by one, and each second liquid outlet is communicated with a corresponding main liquid tank 2011 a. In a specific application, the second liquid outlet may be connected to a waste liquid bottle through a liquid suction pipe, and the waste liquid bottle is connected to a liquid suction pump through a conduit, and since the liquid tank unit 2011 is sealed by a cover plate, a negative pressure may be formed in the main liquid tank 2011a and the second liquid separation tank 2011b in the liquid tank unit 2011 by using the suction effect of the liquid suction pump.
At least one liquid sucking needle unit 203 is in one-to-one correspondence with at least one liquid groove unit 2011, each liquid sucking needle unit 203 comprises a plurality of rows of liquid sucking needles 2031, and the plurality of rows of liquid sucking needles 2031 are in one-to-one correspondence with a plurality of second liquid separating grooves 2011 b; each of the liquid suction needles 2031 is disposed on the second needle holder 201 in a penetrating manner, and has an upper end communicating with the second liquid separation tank 2011b and a lower end penetrating from the bottom surface of the second needle holder 201.
That is, the liquid suction needles 2031, the second liquid separation tank 2011b, the main liquid tank 2011a, and the second liquid outlet are sequentially connected, when the second liquid outlet is connected to the liquid suction pump, each liquid suction needle 2031 sucks the liquid in each microwell on the microwell plate W to the second liquid separation tank 2011b, flows into the main liquid tank 2011a from the second liquid separation tank 2011b, and finally flows into the second liquid outlet from the main liquid tank 2011a, and is discharged from the second liquid outlet.
It should be noted that, the number of the liquid tank units 2011 and the number of the liquid sucking needles 2031 may be configured according to the need, for example, according to the specification of the micro-well plate W, in the example of fig. 1, two liquid tank units 2011 are arranged side by side, corresponding two second liquid outlets are two, and the two second liquid outlets are respectively communicated with the main liquid tank 2011a in the two liquid tank units 2011, so, when in specific application, each second liquid outlet is respectively connected to one liquid sucking pipeline, and the two liquid sucking pipelines respectively suck the liquid in the two liquid tank units 2011, so that the negative pressure in each liquid tank unit 2011 can be improved, and the liquid sucking effect is further improved. In addition, 8 liquid suction needles 2031 are disposed in each second liquid separation tank 2011b, and thus, the liquid suction requirement of the microplate W of the 8×m (M is a natural number greater than 1) specification can be satisfied.
In this embodiment, the liquid suction head 20 adopts a split structure, when assembling, the scraps in the second needle stand 201 can be cleaned first, so that after the scraps, burrs and the like in the second needle stand 201 are avoided, the second cover plate 202 and the second needle stand 201 are assembled, and thus, the problems of blockage and the like of the liquid suction needle 2031, which are easily caused by the scraps, the burrs and the like, can be avoided. In addition, after pipette tip 2031 is clogged or used for a certain period of time, second cover 202 may be opened to clear away dirt and the like in pipette tip 2031 and the liquid tank unit on second hub 201.
Further, the liquid tank unit 2011 includes a main liquid tank 2011a and a plurality of second liquid separation tanks 2011b, the plurality of second liquid separation tanks 2011b are all communicated with the main liquid tank 2011a, and the bottom surface of the main liquid tank 2011a lower than the bottom surface of each second liquid separation tank 2011b, so that the liquid sucked by the liquid suction needle 2031 is converged to the main liquid tank 2011a through each second liquid separation tank 2011b, enters the second liquid outlet from the main liquid tank 2011a, and is finally discharged through the second liquid outlet, and since the bottom surface of the main liquid tank 2011a is lower than the bottom surface of each second liquid separation tank 2011b, the liquid can only flow from the high second liquid separation tank 2011b to the main liquid tank 2011a, and the problems that the liquid suction speed of the liquid suction needle 2031 close to the liquid outlet is high, the liquid suction speed of the liquid suction needle 2031 far away from the liquid outlet is slow and the like in the related art are avoided, and the consistency of the liquid suction speed of each liquid suction needle 2031 is ensured to be better.
In an embodiment of the present invention, the medical device further includes at least one second connector 204, at least one second connector 204 corresponds to at least one second liquid outlet one by one, and each second connector 204 is disposed on the second needle stand 201 and is connected with the corresponding second liquid outlet in a sealing manner.
That is, each second liquid outlet is connected to one second connector 204, so that the liquid suction pipeline can be connected to the liquid suction pump through the second connector 204, and the connection is convenient.
Referring to fig. 12, in one embodiment of the present invention, the second liquid outlet is advantageously provided on the side surface of second hub 201 below bottom surface S202 of second liquid separation groove 2011 b. In this way, on the one hand, when the liquid in the main liquid tank 2011a is sucked out from the second liquid outlet, the liquid in the second liquid separation tank 2011b can be ensured to flow into the main liquid tank 2011a entirely and be sucked out from the main liquid tank 2011 a. On the other hand, if the second liquid outlet is too high, the upper liquid in the second liquid separating tank 2011b is directly sucked out from the second liquid outlet, so that the liquid sucking speed of the liquid sucking needle 2031 close to the second liquid outlet is fast, and the liquid sucking speed of the liquid sucking needle 2031 far away from the second liquid outlet is slow, in this example, the second liquid outlet is lower than the bottom surface S202 of the second liquid separating tank 2011b, so that the liquid always flows from the main liquid tank 2011a to the second liquid outlet, and the consistency of the liquid sucking speeds of the liquid sucking needles 2031 can be further improved.
Referring to fig. 12, in some embodiments of the present invention, the upper end of each of the liquid sucking needles 2031 is higher than the bottom surface S202 of the second liquid dividing groove 2011 b. As described above, when dirt is accumulated in the second liquid separation tank 2011b, the dirt is generally located on the bottom surface S202 of the second liquid separation tank 2011b, and therefore, the dirt is not likely to enter the liquid suction needle 2031, and the liquid suction needle 2031 is not likely to be clogged.
Illustratively, second cover 202 is coupled to second hub 201 via a plurality of screws, which facilitates coupling and decoupling between second cover 202 and second hub 201, while providing a secure structure.
Advantageously, a sealing ring may be disposed between second cover plate 202 and the top surface of second hub 201, and when second cover plate 202 and second hub 201 are fixed by screw connection, sealing ring may seal the gap between second cover plate 202 and second hub 201, so as to achieve better sealing effect.
Referring to fig. 1 to 2, in one embodiment of the present invention, a tray table 402 is disposed below the tray 30, a sliding frame 403 is disposed on the tray table 402 and is slidable in a left-right direction, a connecting arm extends from the sliding frame 403 and one side, the connecting arm is fixedly connected to the sliding block 316, and the tray 30 is mounted on the sliding frame 403. In this way, on the one hand, the tray 30 can be supported by the carriage 403 so that the tray 30 is kept stable, and on the other hand, the tray 30 can be slid by the carriage 403, and thus, the movement of the tray 30 can be made smoother and more reliable.
Illustratively, the liquid receiving tank 4021 is disposed on the supporting platform 402, and the liquid receiving tank 4021 is located under the liquid injection head 10, so that the dropped liquid can be collected by the liquid receiving tank 4021 when the liquid injection head 10 hangs the liquid, and pollution to other positions of the working area is avoided.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
The foregoing description is only of the preferred embodiments of the present invention and is not intended to limit the scope of the invention, and all equivalent structural changes made by the description of the present invention and the accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the invention.
Claims (7)
1. A plate washer, comprising:
The liquid injection mechanism comprises a liquid injection head;
The liquid suction mechanism comprises a liquid suction head and a first driving mechanism, the liquid suction head and the liquid injection head are arranged side by side along the left-right direction, and the first driving mechanism is connected with the liquid suction head so as to drive the liquid suction head to move up and down;
The tray mechanism comprises a tray and a second driving mechanism, the tray is suitable for loading the micro-pore plates, and the second driving mechanism is connected with the tray to drive the tray to rotate at the lower positions of the liquid suction head and the liquid injection head;
the liquid injection head comprises:
The first needle seat is provided with a plurality of first liquid distribution tanks and at least one communication tank on the top surface, and the plurality of first liquid distribution tanks are communicated through at least one communication tank; the inside of the first needle seat is provided with a liquid injection channel, one end of the liquid injection channel is formed into a liquid inlet, the liquid inlet is positioned on the side surface of the needle seat, the other end of the liquid injection channel is formed into a first liquid outlet, and the first liquid outlet upwards penetrates through at least one communication groove;
the first cover plate is detachably arranged on the top surface of the first needle seat and seals the first liquid distributing groove and the communication groove;
The liquid injection needles are in one-to-one correspondence with the first liquid distribution tanks, each liquid injection needle penetrates through the first needle seat, the upper ends of the liquid injection needles are communicated with the first liquid distribution tanks, and the lower ends of the liquid injection needles penetrate out of the bottom surface of the first needle seat;
the upper end of each liquid injection needle is higher than the bottom surface of the first liquid distribution groove;
the first driving mechanism includes:
The support is provided with a screw rod and a first guide rod which extend in the up-down direction;
the floating plate is in threaded sleeve fit with the screw rod, and the floating plate is in sliding sleeve fit with the first guide rod;
The output shaft of the first motor extends along the up-down direction and is connected with the screw rod so as to drive the screw rod to rotate and enable the floating plate to move up and down;
the liquid suction head is connected with the floating plate so as to move up and down along with the floating plate.
2. The plate washer of claim 1, wherein the second drive mechanism comprises:
the transmission assembly comprises a driving wheel, a driven wheel and a transmission belt, and the transmission belt is sleeved between the driving wheel and the driven wheel;
the clamping block is clamped on the transmission belt;
the guide assembly comprises a first support, a second guide rod connected between the first support and the second support and a sliding block sleeved on the second guide rod in a sliding manner, the second guide rod extends along the left-right direction, and the sliding block is fixedly connected with the clamping block;
the output shaft of the second motor extends along the front-back direction and is connected with the driving wheel so as to drive the driving wheel to rotate and enable the driving belt to drive the clamping block and the sliding block to move left and right;
The tray is fixedly connected with the sliding block so as to slide left and right along with the sliding block.
3. The plate washer of claim 1, wherein the pipette head comprises:
the top surface of the second needle seat is provided with at least one liquid groove unit, each liquid groove unit comprises a main liquid groove and a plurality of second liquid grooves, the second liquid grooves are communicated with the main liquid groove, and the bottom surface of the main liquid groove is lower than the bottom surface of each second liquid groove;
the second cover plate is detachably arranged on the top surface of the second needle seat and seals the liquid tank unit;
at least one second liquid outlet is formed in the second needle seat or the second cover plate, the at least one second liquid outlet corresponds to the at least one liquid tank unit one by one, and each second liquid outlet is communicated with the corresponding main liquid tank;
At least one liquid sucking needle unit, wherein the at least one liquid sucking needle unit corresponds to at least one liquid groove unit one by one, each liquid sucking needle unit comprises a plurality of rows of liquid sucking needles, and the liquid sucking needles correspond to a plurality of second liquid separating grooves one by one; each liquid suction needle penetrates through the second needle seat, the upper end of each liquid suction needle is communicated with the second liquid separation groove, and the lower end of each liquid suction needle penetrates out of the bottom surface of the second needle seat.
4. A plate washer according to claim 3, wherein the second liquid outlet is provided in a side face of the second needle holder and lower than a bottom face of the second liquid dividing groove.
5. A plate washer according to claim 3, wherein the upper end of each of said liquid sucking needles is higher than the bottom surface of said second liquid dividing groove.
6. The plate washer according to claim 2, wherein a pallet is provided below the tray, a carriage slidable in a left-right direction is provided on the pallet, a connecting arm is extended from the carriage and the one side, the connecting arm is fixedly connected with the sliding block, and the tray is mounted on the carriage.
7. The plate washer of claim 6, wherein a liquid receiving trough is provided on the pallet, the liquid receiving trough being located directly below the liquid injection head.
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CN110860540B (en) * | 2018-08-27 | 2021-07-27 | 深圳迎凯生物科技有限公司 | Cleaning method and sample analysis method |
CN109772779B (en) * | 2019-02-12 | 2019-11-08 | 深圳市华科瑞科技有限公司 | A kind of board-washing machine without washing lotion cross contamination |
CN109807106B (en) * | 2019-03-21 | 2024-09-03 | 安图实验仪器(郑州)有限公司 | Full-automatic tray type slide vibration cleaning mechanism |
CN112570357B (en) * | 2020-11-19 | 2022-02-01 | 遵义医药高等专科学校 | 96 orifice plate wash plate device with defoaming function |
CN114178226B (en) * | 2021-11-05 | 2023-05-23 | 山东博科生物产业有限公司 | Plate washer integrating sterilization function of drying plate |
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