CN108207485A - It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method - Google Patents
It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method Download PDFInfo
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- CN108207485A CN108207485A CN201711473016.4A CN201711473016A CN108207485A CN 108207485 A CN108207485 A CN 108207485A CN 201711473016 A CN201711473016 A CN 201711473016A CN 108207485 A CN108207485 A CN 108207485A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
- C05D1/02—Manufacture from potassium chloride or sulfate or double or mixed salts thereof
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Abstract
The invention discloses it is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method, it is characterized in that, it is crushed after rice husk is dried, it is crushed after silkworm excrement is dried, pretreated powdered rice hulls, silkworm excrement and calcium carbonate, potassium dihydrogen sulfate mixing are obtained into culture material, adds water, wet culture material is fitted into material bag, sack is fastened, by gained bacterium bag high-temperature sterilization；The flat mushroom strain activated is inoculated on level-one kind culture medium, first class inoculum is cultivated to obtain, inoculates and second class inoculum is cultivated to obtain in second-generation culture medivm；Aseptically, oyster mushroom liquid spawn is inoculated into bacterium bag, gets through stomata at bacterium bag both ends, culture bacterium germination in constant incubator, stirring is primary during mycelia material feeding 5cm, increases oxygen content, treats that mycelia reaches physiological maturity；After treating bacterium germination, bacterium bag is transported to out mushroom shed, water spray control relative air humidity applies scattering light stimulus flower bud, and daily ventilation harvests before spore is not launched, with a little culture medium during harvesting, so as to change of tide fruiting.
Description
Technical field
The present invention relates to planting edible mushroom fields, and in particular to it is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom
Method.
Background technology
Edible fungi growth is influenced by external condition, even same fungi under different condition of culture, to straw of the same race
The degradation capability of lignin and cellulose in stalk is also significantly different, thus causes multifarious to the treatment effect of stalk.Its
Middle carbon source and nitrogen source are the factor the most main for influencing Fungi Degrading Lignin again.Now mostly with glucose, sucrose, corn flour
Etc. the carbon source for making deep drainpipe, the nitrogen source as deep drainpipe such as peptone, dusty yeast, analysis for soybean powder does carbon source using powdered rice hulls,
Silkworm excrement makees nitrogen source and carries out deep drainpipe to fungi.Rice husk is Rice producing by-product, and silkworm excrement is the by-product of Sericulture, usually profit
It is very low with rate.The two is used as to the carbon and nitrogen sources of edible mushroom culture, not only abundant raw materials, cheap, and can bring very
Good economic interests.
Invention content
The invention mainly solves the technical problem of providing it is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom side
Method, it is high, good in economic efficiency according to this method culture oyster mushroom cultivating rate.
The technical problems to be solved by the invention are realized using following technical solution：
It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method, which is characterized in that carry out according to the following steps：
（1）The pretreatment of culture material and being produced as follows for bacterium bag：
Fresh rice husk is dried, crushed 80 mesh sieve；By silkworm excrement, drying to constant weight in 80-85 DEG C of drying box, crushed 60 mesh
Sieve；4-5% powdered rice hulls, 1-2% silkworm excrements and 0.9-1% calcium carbonate, 0.9-1% potassium dihydrogen sulfate mixings is taken to obtain culture material, according to material water
Than 1:1.2 plus water, wet culture material is fitted into material bag, tie-down sack, bacterium bag is sterilized 1-2h in 120-122 DEG C of autoclave,
Cooling；
Material bag is low-pressure high-density polyethylene polybag, and specification is 22cm × 45cm × 0.0015cm；
（2）Preparing for oyster mushroom liquid spawn is as follows：
Level-one kind, second-generation culture medivm formula are：Glucose 2%, peptone 0.2%, MgSO4·7H2O 0.05%、KH2PO4
0.05%th, 10% Pleurotus eryngii mushroom bran extracting solution, 40% beer yeast fermenting liquid；
The slant strains activated are inoculated on level-one kind culture medium（Flat mushroom strain inoculum concentration is 15-20%）, in 170-200r/
6-7d is cultivated under the conditions of min, 24-26 DEG C and obtains first class inoculum, 8-10% is inoculated into second-generation culture medivm by volume, identical
Under the conditions of culture 4-5d obtain second class inoculum；
（3）The inoculated and cultured of oyster mushroom liquid spawn is as follows：
Aseptically operate, it will（2）Middle gained oyster mushroom liquid spawn is inoculated into（1）In middle gained bacterium bag, at bacterium bag both ends
The venthole of depth 6-10cm is beaten, cultivates bacterium germination in constant incubator at 24-26 DEG C, stirring is primary during mycelia material feeding 5cm, increase
Oxygen content treats that mycelia reaches physiological maturity, when there is caking protuberance phenomenon, carries out management of producing mushroom.
（4）Management of producing mushroom and harvesting are as follows：
After treating bacterium germination, bacterium bag is transported to out mushroom shed, water spray control relative air humidity is 85-90%, temperature is 13-18 DEG C,
Apply scattering light stimulus flower bud, daily ventilation 1-2h, mushroom carpophore cap edge it is involute, do not launch before spore in time
Harvesting, with a little culture medium during harvesting, so as to change of tide fruiting.
Wherein, the preparation of Pleurotus eryngii mushroom bran extracting solution, beer yeast fermenting liquid prepare it is as follows：
It is dried dry at 60-62 DEG C without the Pleurotus eryngii mushroom bran gone mouldy, crushed 80 mesh sieve, boiling is added to boil 20-25min, mistake
Filter, adds water to obtain Pleurotus eryngii mushroom bran extracting solution；
Malt is crushed, 1:4-5 adds in water, and be saccharified 3-4h at 64-66 DEG C, and brewer's wort is obtained by filtration, and goes out at 120-122 DEG C
Bacterium 20-22min accesses brewer's yeast, and 1-2d is cultivated at 24-26 DEG C, and centrifugation 20-23min takes supernatant, water is added to obtain beer ferment
Female zymotic fluid.
The present invention reaction mechanism and have the beneficial effect that：
Using powdered rice hulls as carbon source, silkworm excrement it is nitrogen source, mycelia grows fine in culture medium, and the biological efficiency of oyster mushroom fruiting is big；
Pleurotus eryngii mushroom bran extracting solution, beer yeast fermenting liquid are added in culture medium, Pleurotus ostreatus yield and bacterium can be significantly improved
Ball number（Peloton density）；Materials bag cultivating oyster mushroom while stomata is got through, the with short production cycle of oyster mushroom, pollution rate are low, biology effect
Rate is high, good in economic efficiency；The mode of punching ventilation can meet the needs of Growth of Pleurotus Mycelium is to oxygen, can reduce water-in-bag point
It scatters and disappears.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment
The technical problems to be solved by the invention are realized using following technical solution：
It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method, which is characterized in that carry out according to the following steps：
（1）The pretreatment of culture material and being produced as follows for bacterium bag：
Fresh rice husk is dried, crushed 80 mesh sieve；By silkworm excrement, drying to constant weight in 80-85 DEG C of drying box, crushed 60 mesh
Sieve；4% powdered rice hulls, 1% silkworm excrement and 0.9% calcium carbonate, 1% potassium dihydrogen sulfate mixing is taken to obtain culture material, according to material-water ratio 1:1.2 plus
Wet culture material is fitted into material bag by water, fastens sack, and bacterium bag is sterilized 1h in 120-122 DEG C of autoclave, cooling；
Material bag is low-pressure high-density polyethylene polybag, and specification is 22cm × 45cm × 0.0015cm；
（2）Preparing for oyster mushroom liquid spawn is as follows：
Level-one kind, second-generation culture medivm formula are：Glucose 2%, peptone 0.2%, MgSO4·7H2O 0.05%、KH2PO4
0.05%th, 10% Pleurotus eryngii mushroom bran extracting solution, 40% beer yeast fermenting liquid；
The slant strains activated are inoculated on level-one kind culture medium（Flat mushroom strain inoculum concentration is 20%）, 170-200r/min,
6d is cultivated under the conditions of 24-26 DEG C and obtains first class inoculum, 8% is inoculated into second-generation culture medivm, cultivates under the same conditions by volume
4d obtains second class inoculum；
（3）The inoculated and cultured of oyster mushroom liquid spawn is as follows：
Aseptically operate, it will（2）Middle gained oyster mushroom liquid spawn is inoculated into（1）In middle gained bacterium bag, at bacterium bag both ends
The venthole of depth 6cm is beaten, cultivates bacterium germination in constant incubator at 24-26 DEG C, stirring is primary during mycelia material feeding 5cm, increase oxygen
Gas content treats that mycelia reaches physiological maturity, when there is caking protuberance phenomenon, carries out management of producing mushroom.
（4）Management of producing mushroom and harvesting are as follows：
After treating bacterium germination, bacterium bag is transported to out mushroom shed, water spray control relative air humidity is 85-90%, temperature is 13-18 DEG C,
Apply scattering light stimulus flower bud, daily ventilation 1h, mushroom carpophore cap edge it is involute, do not launch spore before adopt in time
It receives, with a little culture medium during harvesting, so as to change of tide fruiting.
Wherein, the preparation of Pleurotus eryngii mushroom bran extracting solution, beer yeast fermenting liquid prepare it is as follows：
It is dried dry at 60-62 DEG C without the Pleurotus eryngii mushroom bran gone mouldy, crushed 80 mesh sieve, boiling is added to boil 25min, filter,
Water is added to obtain Pleurotus eryngii mushroom bran extracting solution；
Malt is crushed, 1:5 add in water, and be saccharified 3h at 64-66 DEG C, and brewer's wort is obtained by filtration, and sterilizes at 120-122 DEG C
22min accesses brewer's yeast, and 1d is cultivated at 24-26 DEG C, and centrifugation 23min takes supernatant, water is added to obtain beer yeast fermenting liquid.
Claims (6)
1. it is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method, which is characterized in that crushed after rice husk is dried,
It is crushed after silkworm excrement is dried, pretreated powdered rice hulls, silkworm excrement and calcium carbonate, potassium dihydrogen sulfate mixing is obtained into culture material, add water,
Wet culture material is fitted into material bag, sack is fastened, by gained bacterium bag high-temperature sterilization；Inoculation has activated on level-one kind culture medium
Flat mushroom strain cultivates to obtain first class inoculum, inoculates and second class inoculum is cultivated to obtain in second-generation culture medivm；Aseptically, will
Oyster mushroom liquid spawn is inoculated into bacterium bag, and stomata is got through at bacterium bag both ends, bacterium germination, mycelia material feeding 5cm are cultivated in constant incubator
When stirring it is primary, increase oxygen content, treat that mycelia reaches physiological maturity；After treating bacterium germination, bacterium bag is transported to out mushroom shed, is sprayed water
Relative air humidity is controlled, applies scattering light stimulus flower bud, daily ventilation harvests in time before spore is not launched, harvests
When with a little culture medium, so as to change of tide fruiting.
2. according to claim 1 a kind of using powdered rice hulls, silkworm excrement as the method for the culture oyster mushroom of culture material, feature exists
In the pretreatment of culture material and being produced as follows for bacterium bag：
Fresh rice husk is dried, crushed 80 mesh sieve；By silkworm excrement, drying to constant weight in 80-85 DEG C of drying box, crushed 60 mesh
Sieve；4-5% powdered rice hulls, 1-2% silkworm excrements and 0.9-1% calcium carbonate, 0.9-1% potassium dihydrogen sulfate mixings is taken to obtain culture material, according to material water
Than 1:1.2 plus water, wet culture material is fitted into material bag, tie-down sack, bacterium bag is sterilized 1-2h in 120-122 DEG C of autoclave,
Cooling；
Material bag is low-pressure high-density polyethylene polybag, and specification is 22cm × 45cm × 0.0015cm.
3. according to claim 1 a kind of using powdered rice hulls, silkworm excrement as the method for the culture oyster mushroom of culture material, feature exists
In preparing for oyster mushroom liquid spawn is as follows：
Level-one kind, second-generation culture medivm formula are：Glucose 2%, peptone 0.2%, MgSO4·7H2O 0.05%、KH2PO4
0.05%th, 10% Pleurotus eryngii mushroom bran extracting solution, 40% beer yeast fermenting liquid；
The slant strains activated are inoculated on level-one kind culture medium（Flat mushroom strain inoculum concentration is 15-20%）, in 170-200r/
6-7d is cultivated under the conditions of min, 24-26 DEG C and obtains first class inoculum, 8-10% is inoculated into second-generation culture medivm by volume, identical
Under the conditions of culture 4-5d obtain second class inoculum.
4. according to claim 1 a kind of using powdered rice hulls, silkworm excrement as the method for the culture oyster mushroom of culture material, feature exists
In the inoculated and cultured of oyster mushroom liquid spawn is as follows：
It aseptically operates, gained oyster mushroom liquid spawn in power 3 is inoculated into power 2 in gained bacterium bag, at bacterium bag both ends
The venthole of depth 6-10cm is beaten, cultivates bacterium germination in constant incubator at 24-26 DEG C, stirring is primary during mycelia material feeding 5cm, increase
Oxygen content treats that mycelia reaches physiological maturity, when there is caking protuberance phenomenon, carries out management of producing mushroom.
5. according to claim 1 a kind of using powdered rice hulls, silkworm excrement as the method for the culture oyster mushroom of culture material, feature exists
In management of producing mushroom and harvesting are as follows：
After treating bacterium germination, bacterium bag is transported to out mushroom shed, water spray control relative air humidity is 85-90%, temperature is 13-18 DEG C,
Apply scattering light stimulus flower bud, daily ventilation 1-2h, mushroom carpophore cap edge it is involute, do not launch before spore in time
Harvesting, with a little culture medium during harvesting, so as to change of tide fruiting.
6. according to claim 3 a kind of using powdered rice hulls, silkworm excrement as the method for the culture oyster mushroom of culture material, feature exists
Preparing for preparation, beer yeast fermenting liquid in, Pleurotus eryngii mushroom bran extracting solution is as follows：
It is dried dry at 60-62 DEG C without the Pleurotus eryngii mushroom bran gone mouldy, crushed 80 mesh sieve, boiling is added to boil 20-25min, mistake
Filter, adds water to obtain Pleurotus eryngii mushroom bran extracting solution；
Malt is crushed, 1:4-5 adds in water, and be saccharified 3-4h at 64-66 DEG C, and brewer's wort is obtained by filtration, and goes out at 120-122 DEG C
Bacterium 20-22min accesses brewer's yeast, and 1-2d is cultivated at 24-26 DEG C, and centrifugation 20-23min takes supernatant, water is added to obtain beer ferment
Female zymotic fluid.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109089729A (en) * | 2018-09-07 | 2018-12-28 | 贵州高山百益食用菌发展有限公司 | A kind of making and use method of external source corncob nutrition packet |
| CN113079951A (en) * | 2021-05-24 | 2021-07-09 | 宁德师范学院 | Preparation method of wide-substrate-adaptability edible fungus liquid strain |
| CN115426872A (en) * | 2020-05-12 | 2022-12-02 | 株式会社乐乐 | Culture medium, fungal bed, bagged fungal bed, culture medium production method, fungal bed production method, and bagged fungal bed production method |
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| CN104115668A (en) * | 2013-04-26 | 2014-10-29 | 汤阴县食用菌协会 | Production method for oyster mushrooms rich in selenium and zinc |
| CN104823709A (en) * | 2015-04-22 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Cultivation method of oyster mushrooms based on mixed compost |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104115668A (en) * | 2013-04-26 | 2014-10-29 | 汤阴县食用菌协会 | Production method for oyster mushrooms rich in selenium and zinc |
| CN104823709A (en) * | 2015-04-22 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Cultivation method of oyster mushrooms based on mixed compost |
| CN105237147A (en) * | 2015-09-01 | 2016-01-13 | 广西南宁胜祺安科技开发有限公司 | Culture medium for oyster mushroom and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109089729A (en) * | 2018-09-07 | 2018-12-28 | 贵州高山百益食用菌发展有限公司 | A kind of making and use method of external source corncob nutrition packet |
| CN115426872A (en) * | 2020-05-12 | 2022-12-02 | 株式会社乐乐 | Culture medium, fungal bed, bagged fungal bed, culture medium production method, fungal bed production method, and bagged fungal bed production method |
| CN113079951A (en) * | 2021-05-24 | 2021-07-09 | 宁德师范学院 | Preparation method of wide-substrate-adaptability edible fungus liquid strain |
| CN113079951B (en) * | 2021-05-24 | 2023-09-12 | 宁德师范学院 | Preparation method of wide-matrix adaptive edible fungus liquid strain |
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