CN108195809B - Quick biological trace developing agent - Google Patents
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- CN108195809B CN108195809B CN201711430542.2A CN201711430542A CN108195809B CN 108195809 B CN108195809 B CN 108195809B CN 201711430542 A CN201711430542 A CN 201711430542A CN 108195809 B CN108195809 B CN 108195809B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/80—Mixing plants; Combinations of mixers
- B01F33/81—Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles
- B01F33/811—Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles in two or more consecutive, i.e. successive, mixing receptacles or being consecutively arranged
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
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- B01F35/712—Feed mechanisms for feeding fluids
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- B01J4/00—Feed or outlet devices; Feed or outlet control devices
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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Abstract
The invention discloses a quick biological trace developing agent and a preparation method thereof, wherein the quick biological trace developing agent comprises the following raw materials: fluorescent substances, ninhydrin, lanthanum oxide, silver nitrate, polyether F68 and ethanol; the pH value of the biological trace rapid revealing agent is 4.5-6.5; the fluorescent substance is eosin Y, fluorescein or calcein; the fingerprint of the quick biological trace revealing agent is quick in revealing time, can be naturally revealed within 30 seconds, does not need a developing box or a developing instrument, is simple and convenient to operate when in use, and can be sprayed on a detected object by spray irrigation and packaging; the fingerprint can be seen by naked eyes without other expensive optical instruments, such as laser or multiband light sources; the quick biological trace developing agent has wide application range, can develop fingerprints of human bodies, and has quick developing function on body fluids secreted by the human bodies, such as saliva, blood, urine, seminal plaques and female secretion.
Description
Technical Field
The invention relates to the technical field of trace detection, in particular to a quick biological trace developing agent.
Background
The fingerprint technology is developed more slowly than other fields of court science in recent years, the DNA technology and the poison detection technology are developed rapidly in recent years, and the fingerprint developing technology is still based on the traditional physical method, chemical method and optical developing method, wherein the traditional physical method is commonly used as a powder developing method and an iodine fumigation developing method. The physical method is to show fingerprint lines by utilizing the adsorption effect of latent sweat fingerprints on powder materials, and the traditional chemical methods mainly comprise a ninhydrin display method, a DFO display method, a '502' fuming method, a tetramethyl benzidine display method and the like. The method mainly utilizes a chemical reagent and amino acid in the fingerprint to generate a color reaction to generate a compound to display the fingerprint, wherein the tetramethyl benzidine is used for displaying the potential blood fingerprint, hematoporphyrin in the blood reacts with catalase to release ecological oxygen, and the tetramethyl benzidine is oxidized into tetramethyl benzidine blue, thereby displaying the fingerprint.
The traditional fingerprint showing method has high use frequency in actual cases, the method is used for a long time in the national public security actual combat process, the operation is mature, the carrying is convenient, the material cost is low, and the use frequency in various cases related to fingerprints is high. However, many deficiencies are also observed during use. The development needs longer time, the definition and the resolution ratio are low, and in addition, some methods have certain destructiveness on DNA, so that irreversible damage is caused to later-stage DNA inspection, some important evidences are invalid, and much inconvenience is brought to criminal investigation work. The reasons of diversity (material types and background colors) and residual timeliness of latent fingerprint objects and the like are that the latent fingerprints are displayed by the traditional method and cannot meet the requirements of modern criminal investigation technology. Particularly for fingerprints on difficult objects such as tapes, skin surfaces, building materials, composites, etc.
With the economic form of rapid development in China, criminals have also evolved at a high rate. The form of crime also develops towards intellectualization, specialization and science and technology modernization. The criminal investigation technology has also made clear the demand for fingerprint technology: the developing reagent is required to be more sensitive, the developing time is shorter, the fingerprints can be seen without an instrument, the method is suitable for various environments, the using method is simple, the developing technology is more advanced, the requirement on the quality of the fingerprints on site is lower, the DNA damage is small (the detection of the DNA is not influenced), and the like.
Disclosure of Invention
In order to solve the above problems, it is an object of the present invention to provide a rapid agent for visualizing biological tracks.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a quick biological trace developing agent comprises the following raw materials in parts by weight: 1-2 parts of fluorescent substance, 4-8 parts of ninhydrin, 0.2-1 part of lanthanum oxide, 3-10 parts of silver nitrate, 0.4 part of polyether F680.1 and 90-110 parts of ethanol; the pH value of the biological trace rapid revealing agent is 4.5-6.5; the fluorescent substance is eosin Y, fluorescein or calcein; the quick biological trace developing agent is prepared by the following steps:
①, adding 1-2 parts by weight of fluorescent substance into 45-55 parts by weight of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 4-8 parts by weight of ninhydrin into the mixture until the fluorescent substance is completely dissolved to obtain a first solution;
②, adding 3-10 parts of silver nitrate and 0.2-1 part of lanthanum oxide into 45-55 parts of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 4.5-6.5 by using an alkaline solution, adding 0.1-0.4 part of polyether F68, stirring and mixing uniformly, and filling into an aluminum tank filled with nitrogen to obtain the quick biological trace developing agent.
Preferably, the ethanol is absolute ethanol.
Preferably, the fluorescent substance is calcein.
Preferably, when the fluorescent substance is eosin Y, the pH value of the quick biological trace developing agent is 5.7-6.3; when the fluorescent substance is fluorescein, the pH value of the quick biological trace developing agent is 4.7-5.3; when the fluorescent substance is calcein, the pH value of the quick biological trace developing agent is 5.2-5.8.
Preferably, when the fluorescent substance is eosin Y, the pH of the quick-visualizing agent for biological traces is 6.0; when the fluorescent substance is fluorescein, the pH value of the quick biological trace developing agent is 5.0; when the fluorescent substance is calcein, the pH of the quick-acting agent for biological trace is 5.5.
Preferably, the alkaline solution is a sodium carbonate or potassium carbonate water solution, and the mass concentration of the alkaline solution is 5-10%.
Preferably, the alkaline solution is a plant ash buffer solution; the preparation method of the plant ash buffer solution comprises the following steps: burning straws to obtain ash, mixing and stirring the obtained ash and double distilled water, settling for 5-8 hours, taking supernatant fluid, and filtering to obtain filtrate which is plant ash buffer solution; the mass volume ratio of the ash to the double distilled water is 5-10 g: 100 ml.
A mixing reaction kettle system is adopted in the preferred preparation process, and comprises a first mixing tank, a second mixing tank and a connecting pipeline between the two tank bodies;
a first feeding hopper is arranged on one side of the top of the first mixing tank, a first rotating motor is arranged at the axis of the top of the first mixing tank, a first stirrer is connected to the lower portion of the first rotating motor, one end of a connecting pipeline is connected with the bottom of the first mixing tank, the other end of the connecting pipeline is connected with the top of the second mixing tank, a first control valve is arranged on one side, close to the bottom of the tank, of the connecting pipeline at the bottom of the tank of the first mixing tank, and a circulating pump is further arranged on the connecting pipeline;
the upper part of the second mixing tank body is surrounded with a circle of air bag, the side wall of the joint of the tank body and the air bag is provided with a plurality of through holes, one side of the air bag is connected with an air inlet pipe, the upper part of the air inlet pipe is provided with a second feeding hopper, a vibrating screen which is obliquely installed is arranged below the second feeding hopper, the bottom of one side of the air inlet pipe away from the second mixing tank is provided with a collector, one side of the middle part of the second mixing tank body is provided with an alkaline solution feeding port and a stabilizer feeding port, the other side of the second mixing tank body is provided with a display, the periphery of the second mixing tank body is provided with a circle of heaters; the tank top axle center department of second blending tank is equipped with the second rotating electrical machines, installs the hollow tube on the second rotating electrical machines, and the upper end of hollow tube stretches out the second rotating electrical machines and is equipped with rotary joint, the hollow tube is located and is equipped with two sets of sprinkler on the pipe wall in the second blending tank, and two sets of sprinkler arrange from top to bottom, and the sprinkler on upper portion comprises two first sparge pipes of horizontal symmetry, and first sparge pipe top-down sprays the operation, and the sprinkler of lower part comprises two second sparge pipes of horizontal symmetry, and the second sparge pipe sprays the operation from bottom to top, the second agitator is connected to the hollow tube lower extreme, second blending tank inner wall department is equipped with three pH probe, and three pH probe top-down arranges in proper order, and three pH probe passes through the wire and is connected with the.
Compared with the prior art, the invention has the following advantages:
the quick biological trace developing agent adopts a silver ion-fluorescent substance fluorescence quenching system, achieves a good quick biological trace developing effect through scientific material proportion and adjustment of the pH value of the system, does not need to be detected by an optical instrument, can improve the developing degree and the fluorescence output rate of the quenching system due to the addition of lanthanum oxide which has high refractive index sensitive to light, enables the developing effect to be clearer, and can ensure the chemical stability of other materials due to the addition of polyether F68, so that the system can be stored for 1-2 years under the protection of nitrogen.
The fingerprint of the quick biological trace revealing agent is quick in revealing time, can be naturally revealed within 30 seconds, does not need a developing box or a developing instrument, is simple and convenient to operate when in use, and can be sprayed on a detected object by spray irrigation and packaging; the fingerprint can be seen by naked eyes without other expensive optical instruments, such as laser or multiband light sources; the case solving cost can be greatly reduced, and the method is healthy and environment-friendly, has small harm to the health of users and cannot cause environmental pollution; the quick biological trace developing agent has wide application range, can develop fingerprints of human bodies, and has quick developing function on body fluid secreted by the human bodies, such as saliva, blood, urine, seminal stains and female secretion; the quick biological trace developing agent has complete color system, and different color developing agents can be selected for different trace bearing objects to improve the trace resolution.
The mixed reaction kettle system can well solve the two problems, can fully protect the structures of the fluorescent substance and the silver nitrate, accelerates the dissolving speed of the silver nitrate and the lanthanum oxide, and better and faster obtains the quick biological trace developing agent.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view of the second mixing tank of FIG. 1;
FIG. 3 is a view showing the effect of the quick agent for visualizing biological tracks of example 7 on the palm of a hand;
in the figure, 1, a first mixing tank, 2, a second mixing tank, 3, a connecting pipeline, 4, a first feeding hopper, 5, a first rotating motor, 6, a first stirrer, 7, a second feeding hopper, 8, a vibrating screen, 9, a collector, 10, an air inlet pipe, 11, an air bag, 12, a through hole, 13, a second rotating motor, 14, a hollow pipe, 15, a second stirrer, 16, a first spraying pipe, 17, a second spraying pipe, 18, a heater, 19, a display, 20, an alkaline solution feeding port, 21, a stabilizer feeding port, 22, a circulating pump, 23, a first control valve, 24, a second control valve, 25, a pH probe and 26 are connected in a rotating mode.
Detailed Description
The invention aims to provide a quick biological trace revealing agent and a preparation method thereof, and the quick biological trace revealing agent is realized by the following technical scheme:
a quick biological trace developing agent comprises the following raw materials in parts by weight: 1-2 parts of fluorescent substance, 4-8 parts of ninhydrin, 0.2-1 part of lanthanum oxide, 3-10 parts of silver nitrate, 0.4 part of polyether F680.1 and 90-110 parts of ethanol; the pH value of the biological trace rapid revealing agent is 4.5-6.5; the fluorescent substance is eosin Y, fluorescein or calcein, the eosin Y is red correspondingly, the fluorescein is yellow correspondingly, and the calcein is green correspondingly;
the quick biological trace developing agent is prepared by the following steps:
①, adding 1-2 parts by weight of fluorescent substance into 45-55 parts by weight of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 4-8 parts by weight of ninhydrin into the mixture until the fluorescent substance is completely dissolved to obtain a first solution;
②, adding 3-10 parts of silver nitrate and 0.2-1 part of lanthanum oxide into 45-55 parts of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 4.5-6.5 by using an alkaline solution, adding 0.1-0.4 part of polyether F68, stirring and mixing uniformly, and filling into an aluminum tank filled with nitrogen to obtain the quick biological trace developing agent.
Preferably, the ethanol is absolute ethanol, the absolute ethanol is used as a solvent, the dissolving rate of the fluorescent substance, the ninhydrin, the lanthanum oxide and the silver nitrate can be increased, the ratio change of the concentration in each reagent is small when the pH value is adjusted by adding the pH buffer solution, the fingerprint display intensity of the reagent under low light can be improved by using the absolute ethanol and the lanthanum oxide in a matching way, and the fluorescence output rate is enhanced under the excitation of strong light.
Preferably, the fluorescent substance is calcein.
Preferably, the quick biological trace developing agent uses plant ash buffer solution to adjust the pH value; the preparation method of the plant ash buffer solution comprises the following steps: burning straws to obtain ash, mixing and stirring the obtained ash and double distilled water, settling for 5-8 hours, taking supernatant fluid, and filtering to obtain filtrate which is plant ash buffer solution; the mass volume ratio of the ash to the double distilled water is 5-10 g: 100ml of the optimized plant ash buffer solution can not only adjust the pH value, but also increase the chemical stability of the quick biological trace developing agent, and ensure that the substances are well preserved for 1-2 years in a sealed state.
Preferably, when the fluorescent substance is eosin Y, the pH value of the quick biological trace developing agent is 5.7-6.3; when the fluorescent substance is fluorescein, the pH value of the quick biological trace developing agent is 4.7-5.3; when the fluorescent substance is calcein, the pH value of the quick biological trace developing agent is 5.2-5.8.
Preferably, when the fluorescent substance is eosin Y, the pH of the quick-visualizing agent for biological traces is 6.0; when the fluorescent substance is fluorescein, the pH value of the quick biological trace developing agent is 5.0; when the fluorescent substance is calcein, the pH of the quick-acting agent for biological trace is 5.5.
Preferably, the alkaline solution is a sodium carbonate or potassium carbonate water solution, and the mass concentration of the alkaline solution is 5-10%.
Preferably, the alkaline solution is a plant ash buffer solution; the preparation method of the plant ash buffer solution comprises the following steps: burning straws to obtain ash, mixing and stirring the obtained ash and double distilled water, settling for 5-8 hours, taking supernatant fluid, and filtering to obtain filtrate which is plant ash buffer solution; the mass volume ratio of the ash to the double distilled water is 5-10 g: 100 ml.
As shown in figure 1, the mixing reaction kettle system comprises a first mixing tank 1, a second mixing tank 2 and a connecting pipeline 3 between the two tank bodies, wherein one end of the connecting pipeline 3 is connected with the bottom of the first mixing tank 1, and the other end of the connecting pipeline is connected with the top of the second mixing tank 2.
As shown in fig. 1, a first feeding hopper 4 is arranged on one side of the top of the first mixing tank 1, a first rotating motor 5 is arranged at the axis of the top of the tank body, a first stirrer 6 is connected to the lower part of the first rotating motor 5, and the first rotating motor 5 drives the first stirrer 6 to stir liquid in the tank. The first mixing tank 1 has a first control valve 23 on the connecting pipe 3 near the bottom of the tank, which may be a general-purpose solenoid valve. The connecting pipeline 3 is further provided with a circulating pump 22 for pumping the liquid in the first mixing tank 1 into the second mixing tank 2.
As shown in FIG. 2, a circle of air bags 11 is enclosed at the upper part of the tank body of the second mixing tank 2, and a plurality of through holes 12, preferably three rows of through holes 12, are arranged in sequence from top to bottom on the side wall of the joint of the tank body and the air bags 11. One side of the air bag 11 is connected with an air inlet pipe 10, the upper part of the air inlet pipe 10 is provided with a second feeding hopper 7, a vibrating screen 8 which is obliquely arranged is arranged below the second feeding hopper 7, and the bottom of the air inlet pipe 10 is provided with a collector 9. The material put in the second feeding hopper 7 belongs to the substance silver nitrate which is not easy to dissolve and is easy to oxidize, nitrogen is filled in the air inlet pipe 10 for anti-oxidation protection, the material which accords with the particle size can fall into the air inlet pipe 10 after being screened by the vibrating screen 8, and the material is blown into the air bag 11 through the nitrogen and then enters the tank body through the through holes 12 which are uniformly distributed. The too big material of particle diameter falls into collector 9 through income tuber pipe 10 under the effect of 8 inclination of reciprocating sieve, waits to collect after certain degree can put into the screening again in second hopper 7 to large granule material crushing again, has avoided the waste of material. The pressure of the nitrogen is controlled so that the qualified material can be blown into the air bag 11 without affecting the falling of the large-particle material into the collector 9.
As shown in fig. 2, a second rotating electrical machine 13 is disposed at the top axis of the second mixing tank 2, a hollow pipe 14 is mounted on the second rotating electrical machine 13, a rotary joint 26 is disposed at the upper end of the hollow pipe 14 extending out of the second rotating electrical machine 13, and a liquid substance is introduced into the hollow pipe 14 through the rotary joint 26. Two groups of spraying devices are arranged on the pipe wall of the part of the hollow pipe 14 positioned in the second mixing tank 2, and the two groups of spraying devices are arranged up and down. The upper spraying device is composed of two first spraying pipes 16 which are horizontally symmetrical, the first spraying pipes 16 spray from top to bottom, the lower spraying device is composed of two second spraying pipes 17 which are horizontally symmetrical, and the second spraying pipes 17 spray from bottom to top. Two sets of sprinkler all can rotate under hollow tube 14 drives, and the liquid matter that lets in can evenly spray the jar internal, through-hole 12 is seted up between two sets of sprinklers, forms the violent collision between powder and the liquid drop, can make material and liquid matter intensive mixing, mixes the solution with higher speed. The lower end of the hollow pipe 14 is connected with a second stirrer 15 for stirring the mixed liquid in the tank.
As shown in fig. 2, an alkaline solution feeding port 20 and a stabilizer feeding port 21 are formed at one side of the middle part of the second mixing tank 2, and a display 19 is arranged at the other side. The display 19 may be composed of an existing control module and circuit board, and the pH value may be set by the user for automatic control. The second mixing tank 2 is provided with a ring of heaters 18 at the periphery of the tank body, and the heaters 18 can be heating wires or other heating devices. The second control valve 24 is arranged on the discharge port at the bottom of the tank to control the discharge of the mixed liquid, and the control valve can be a general electromagnetic valve. The inner wall of the second mixing tank 2 is provided with three pH probes 25, and the three pH probes 25 are sequentially arranged from top to bottom and can test the pH values of three different positions of liquid in the tank. When the three pH values are approximately the same, the mixing is completed, the three pH probes 25 are connected with the display 19 through leads, and the pH values of the three probes can be displayed on the display 19. The value detected by the pH probe 25 is converted into an electric signal and transmitted to the display 19, when the value difference of the three pH probes 25 is large, the module of the display 19 is not processed, and when the values of the three pH probes 25 are the same or are close to be within the design range, the module of the display 19 starts to process: and if the set value is not reached, signaling to stop adding the alkaline solution, and if the set value is not reached, signaling to add the alkaline solution again until the set value is reached, and then signaling to require adding the stabilizer.
When the mixing reactor system works, on one hand, the fluorescent substance and the ethanol are put into the first feeding hopper 4 of the first mixing tank 1, on the other hand, the silver nitrate and the lanthanum oxide are put into the second feeding hopper 7 of the second mixing tank 2, and the ethanol is added into the rotary joint 26. The whole mixing and dissolving process in the two mixing tanks is in a light-tight environment. After fluorescent substance and ethanol are fully dissolved and mixed in the first mixing tank 1 to form mixed liquid, when the mixing in the second mixing tank 2 is completed, the first control valve 23 is opened, and the first solution is pumped into the second mixing tank 2 through the circulating pump 22 to be mixed with the original second solution in the second mixing tank 2 for the second time. And after the materials are stirred in the second mixing tank 2 for a set time, adding an alkaline solution to adjust the pH value, continuously stirring in the process, when the values measured by the three pH probes 25 are the same, indicating that the materials are uniformly mixed, at the moment, if the pH value does not reach the set value, continuously adding the alkaline solution to continue stirring until the pH values detected by the three pH probes 25 reach the set value, adding a stabilizer polyether F68, continuously stirring for the set time, opening the second control valve 24, and discharging the biological trace quick-showing agent.
The invention is further described with reference to specific examples.
Example 1
A quick biological trace developing agent comprises the following raw materials: 1kg of fluorescent material, 4kg of ninhydrin, 0.2kg of lanthanum oxide, 3kg of silver nitrate, 680.1kg of polyether and 90kg of ethanol; the pH value of the biological trace rapid-revealing agent is 4.5; the fluorescent substance is eosin Y.
The quick biological trace developing agent is prepared by the following steps:
① adding 1kg of fluorescent substance (eosin Y) into 45kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 4kg of ninhydrin thereto until the fluorescent substance is completely dissolved to obtain a first solution;
② adding 3kg of silver nitrate and 0.2kg of lanthanum oxide into 45kg of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 4.5 by using an alkaline solution, adding 0.1kg of polyether F68, stirring and mixing uniformly, and filling into an aluminum pot filled with nitrogen to obtain the quick biological trace developing agent.
Example 2
A quick biological trace developing agent comprises the following raw materials: 2kg of fluorescent material, 8kg of ninhydrin, 1kg of lanthanum oxide, 10kg of silver nitrate, 680.4 kg of polyether and 110kg of ethanol; the pH value of the biological trace rapid-revealing agent is 6.5; the fluorescent substance is fluorescein.
The quick biological trace developing agent is prepared by the following steps:
① adding 2kg of fluorescent substance into 55kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 8kg of ninhydrin into the mixture until the ninhydrin is completely dissolved to obtain a first solution;
② adding 10kg of silver nitrate and 1kg of lanthanum oxide into 55kg of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 6.5 by using an alkaline solution, adding 0.4kg of polyether F68, stirring and mixing uniformly, and filling into an aluminum pot filled with nitrogen to obtain the quick biological trace developing agent.
Example 3
A quick biological trace developing agent comprises the following raw materials: 1.5kg of fluorescent material, 7kg of ninhydrin, 0.6kg of lanthanum oxide, 8kg of silver nitrate, 680.2kg of polyether and 105kg of ethanol; the pH value of the biological trace rapid-revealing agent is 5.5; the fluorescent substance is calcein.
The quick biological trace developing agent is prepared by the following steps:
① adding 1.5kg of fluorescent substance into 52.5kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 7kg of ninhydrin into the mixture until the fluorescent substance is completely dissolved to obtain a first solution;
② adding 8kg of silver nitrate and 0.6kg of lanthanum oxide into 52.5kg of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 5.5 by using an alkaline solution, adding 0.2kg of polyether F68, uniformly stirring, and filling into an aluminum pot filled with nitrogen to obtain the quick biological trace developing agent.
Example 4
A quick biological trace developing agent comprises the following raw materials: 1.2kg of fluorescent material, 4kg of ninhydrin, 0.5kg of lanthanum oxide, 4kg of silver nitrate, 680.3 kg of polyether and 95kg of absolute ethyl alcohol; the pH value of the biological trace rapid-revealing agent is 5.7; the fluorescent substance is eosin Y.
The quick biological trace developing agent is prepared by the following steps:
① adding 1.2kg of fluorescent substance (eosin Y) into 47.5kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 4kg of ninhydrin thereto until the fluorescent substance is completely dissolved to obtain a first solution;
② adding 4kg of silver nitrate and 0.5kg of lanthanum oxide into 47.5kg of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 5.7 by using an alkaline solution, adding 0.3kg of polyether F68, uniformly stirring, and filling into an aluminum tank filled with nitrogen to obtain a quick biological trace developing agent;
the alkaline solution is a sodium carbonate aqueous solution, and the mass concentration of the alkaline solution is 5%.
Example 5
A quick biological trace developing agent comprises the following raw materials: 1.6kg of fluorescent material, 5kg of ninhydrin, 0.6kg of lanthanum oxide, 6kg of silver nitrate, 680.2kg of polyether and 105kg of absolute ethyl alcohol; the pH value of the biological trace rapid-revealing agent is 4.7; the fluorescent substance is fluorescein.
The quick biological trace developing agent is prepared by the following steps:
① adding 1.6kg of fluorescent substance into 52.5kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 5kg of ninhydrin into the mixture until the fluorescent substance is completely dissolved to obtain a first solution;
② adding 6kg of silver nitrate and 0.6kg of lanthanum oxide into 52.5kg of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 4.7 by using an alkaline solution, adding 0.2kg of polyether F68, uniformly stirring, and filling into an aluminum tank filled with nitrogen to obtain a quick biological trace developing agent;
the alkaline solution is a potassium carbonate water solution, and the mass concentration of the alkaline solution is 10%.
Example 6
A quick biological trace developing agent comprises the following raw materials: 1.8kg of fluorescent material, 7kg of ninhydrin, 0.7kg of lanthanum oxide, 8kg of silver nitrate, 680.25kg of polyether and 100kg of anhydrous ethyl; the pH value of the biological trace rapid-revealing agent is 5.8; the fluorescent substance is calcein.
The quick biological trace developing agent is prepared by the following steps:
① adding 1.8kg of fluorescent substance into 50kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 7kg of ninhydrin into the mixture until the fluorescent substance is completely dissolved to obtain a first solution;
② adding 8kg of silver nitrate and 0.7kg of lanthanum oxide into 50kg of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 5.8 by using an alkaline solution, adding 0.25kg of polyether F68, uniformly stirring, and filling into an aluminum tank filled with nitrogen to obtain a quick biological trace developing agent;
the alkaline solution is a sodium carbonate aqueous solution, and the mass concentration of the alkaline solution is 8%.
Example 7
A quick biological trace developing agent comprises the following raw materials: 1.5kg of fluorescent material, 5kg of ninhydrin, 0.5kg of lanthanum oxide, 5kg of silver nitrate, 680.2kg of polyether and 100kg of absolute ethyl alcohol; the pH value of the biological trace rapid-revealing agent is 6.0; the fluorescent substance is eosin Y;
the quick biological trace developing agent is prepared by the following steps:
① adding 1.5kg of fluorescent substance (eosin Y) into 50kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 5kg of ninhydrin thereto until the fluorescent substance is completely dissolved to obtain a first solution;
② adding silver nitrate 5kg and lanthanum oxide 0.5kg into ethanol 50kg, heating and stirring to dissolve completely, and controlling the heating temperature within 65 deg.C to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 6.0 by using an alkaline solution, adding 0.2kg of polyether F68, uniformly stirring, and filling into an aluminum tank filled with nitrogen to obtain a quick biological trace developing agent;
the alkaline solution is a plant ash buffer solution; the preparation method of the plant ash buffer solution comprises the following steps: burning straws to obtain ash, mixing the obtained ash with double distilled water, stirring, settling for 5 hours, taking supernatant fluid, and filtering to obtain filtrate which is plant ash buffer solution; the mass volume ratio of the ash to the double distilled water is 5 g: 100 ml.
The palm prints are shown by the result of using the quick biological trace revealing agent, and the result is shown in figure 1, so that the patterns are clear and visible to the naked eye.
Example 8
A quick biological trace developing agent comprises the following raw materials: 1.5kg of fluorescent material, 5kg of ninhydrin, 0.5kg of lanthanum oxide, 5kg of silver nitrate, 680.2kg of polyether and 100kg of absolute ethyl alcohol; the pH value of the biological trace rapid-revealing agent is 5.0; the fluorescent substance is fluorescein;
the quick biological trace developing agent is prepared by the following steps:
① adding 1.5kg of fluorescent substance into 50kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 5kg of ninhydrin into the mixture until the fluorescent substance is completely dissolved to obtain a first solution;
② adding silver nitrate 5kg and lanthanum oxide 0.5kg into ethanol 50kg, heating and stirring to dissolve completely, and controlling the heating temperature within 65 deg.C to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 5.0 by using an alkaline solution, adding 0.2kg of polyether F68, uniformly stirring, and filling into an aluminum tank filled with nitrogen to obtain a quick biological trace developing agent;
the alkaline solution is a plant ash buffer solution; the preparation method of the plant ash buffer solution comprises the following steps: burning straws to obtain ash, mixing the obtained ash with double distilled water, stirring, settling for 8 hours, taking supernatant fluid, and filtering to obtain filtrate which is plant ash buffer solution; the mass volume ratio of the ash to the double distilled water is 10 g: 100 ml.
Example 9
A quick biological trace developing agent comprises the following raw materials: 1.5kg of fluorescent material, 5kg of ninhydrin, 0.5kg of lanthanum oxide, 5kg of silver nitrate, 680.0 kg of polyether F680 and 100kg of anhydrous ethyl; the pH value of the biological trace rapid-revealing agent is 5.5; the fluorescent substance is calcein;
the quick biological trace developing agent is prepared by the following steps:
① adding 1.5kg of fluorescent substance into 50kg of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 5kg of ninhydrin into the mixture until the fluorescent substance is completely dissolved to obtain a first solution;
② adding silver nitrate 5kg and lanthanum oxide 0.5kg into ethanol 50kg, heating and stirring to dissolve completely, and controlling the heating temperature within 65 deg.C to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 5.5 by using an alkaline solution, adding 0.20kg of polyether F68, uniformly stirring, and filling into an aluminum tank filled with nitrogen to obtain a quick biological trace developing agent;
the alkaline solution is a plant ash buffer solution; the preparation method of the plant ash buffer solution comprises the following steps: burning straws to obtain ash, mixing the obtained ash with double distilled water, stirring, settling for 6 hours, taking supernatant, and filtering to obtain filtrate which is plant ash buffer solution; the mass volume ratio of the ash to the double distilled water is 6 g: 100 ml.
Claims (7)
1. A quick-acting agent for developing biological traces, which is characterized in that: the composite material comprises the following raw materials in parts by weight: 1-2 parts of fluorescent substance, 4-8 parts of ninhydrin, 0.2-1 part of lanthanum oxide, 3-10 parts of silver nitrate, 0.4 part of polyether F680.1 and 90-110 parts of ethanol; the pH value of the biological trace rapid revealing agent is 4.5-6.5; the fluorescent substance is eosin Y, fluorescein or calcein;
the quick biological trace developing agent is prepared by the following steps:
①, adding 1-2 parts by weight of fluorescent substance into 45-55 parts by weight of ethanol, stirring and mixing until the fluorescent substance is completely dissolved, and then adding 4-8 parts by weight of ninhydrin into the mixture until the fluorescent substance is completely dissolved to obtain a first solution;
②, adding 3-10 parts of silver nitrate and 0.2-1 part of lanthanum oxide into 45-55 parts of ethanol, heating and stirring until the silver nitrate and the lanthanum oxide are completely dissolved, and controlling the heating temperature within 65 ℃ to obtain a second solution;
③, mixing the first solution obtained in the step ① and the second solution obtained in the step ② to obtain a mixed solution, adjusting the pH of the mixed solution to 4.5-6.5 by using an alkaline solution, adding 0.1-0.4 part of polyether F68, stirring and mixing uniformly, and filling into an aluminum tank filled with nitrogen to obtain a quick biological trace developing agent;
a mixing reaction kettle system is adopted in the preparation process, and the mixing reaction kettle system comprises a first mixing tank (1), a second mixing tank (2) and a connecting pipeline (3) between the two tank bodies;
a first feeding hopper (4) is arranged on one side of the top of the first mixing tank (1), a first rotating motor (5) is arranged at the top axle center of the first mixing tank (1), a first stirrer (6) is connected to the lower portion of the first rotating motor (5), one end of the connecting pipeline (3) is connected with the bottom of the first mixing tank (1), the other end of the connecting pipeline is connected with the top of the second mixing tank (2), a first control valve (23) is arranged on one side, close to the bottom of the tank, of the connecting pipeline (3) at the bottom of the tank of the first mixing tank (1), and a circulating pump (22) is further arranged on the connecting pipeline (3);
a circle of air bag (11) is arranged on the upper portion of the tank body of the second mixing tank (2), a plurality of through holes (12) are formed in the side wall of the joint of the tank body and the air bag (11), an air inlet pipe (10) is connected to one side of the air bag (11), a second feeding hopper (7) is arranged on the upper portion of the air inlet pipe (10), a vibrating screen (8) which is installed in an inclined mode is arranged below the second feeding hopper (7), a collector (9) is arranged at the bottom of one side, away from the second mixing tank (2), of the air inlet pipe (10), an alkaline solution feeding port (20) and a stabilizer feeding port (21) are formed in one side of the middle of the tank body of the second mixing tank (2), a display (19) is arranged on the other side of the second mixing tank (2), a circle of heater (18) is arranged on the; a second rotating motor (13) is arranged at the top axis of the second mixing tank (2), a hollow pipe (14) is mounted on the second rotating motor (13), the upper end of the hollow pipe (14) extends out of the second rotating motor (13) and is provided with a rotary joint (26), two groups of spraying devices are arranged on the pipe wall of the hollow pipe (14) in the second mixing tank (2), the two groups of spraying devices are arranged up and down, the spraying device at the upper part consists of two first spraying pipes (16) which are horizontally symmetrical, the first spraying pipes (16) carry out spraying operation from top to bottom, the spraying device at the lower part consists of two second spraying pipes (17) which are horizontally symmetrical, the second spraying pipes (17) carry out spraying operation from bottom to top, the lower end of the hollow pipe (14) is connected with a second stirrer (15), three pH probes (25) are arranged at the inner wall of the second mixing tank (2), and the three pH probes (25) are sequentially arranged from top to bottom, the three pH probes (25) are connected with the display (19) through leads.
2. The agent for rapidly developing biological tracks according to claim 1, wherein: the ethanol is absolute ethanol.
3. The agent for rapidly developing biological tracks according to claim 1, wherein: the fluorescent substance is calcein.
4. The agent for rapidly developing biological tracks according to claim 1, wherein: when the fluorescent substance is eosin Y, the pH value of the quick biological trace developing agent is 5.7-6.3; when the fluorescent substance is fluorescein, the pH value of the quick biological trace developing agent is 4.7-5.3; when the fluorescent substance is calcein, the pH value of the quick biological trace developing agent is 5.2-5.8.
5. The agent for rapidly developing biological tracks according to claim 1, wherein: when the fluorescent substance is eosin Y, the pH of the quick-visualization agent for biological traces is 6.0; when the fluorescent substance is fluorescein, the pH value of the quick biological trace developing agent is 5.0; when the fluorescent substance is calcein, the pH of the quick-acting agent for biological trace is 5.5.
6. The agent for rapidly developing biological tracks according to claim 1, wherein: the alkaline solution is a sodium carbonate or potassium carbonate water solution, and the mass concentration of the alkaline solution is 5-10%.
7. The agent for rapidly developing biological tracks according to claim 1, wherein: the alkaline solution is a plant ash buffer solution; the preparation method of the plant ash buffer solution comprises the following steps: burning straws to obtain ash, mixing and stirring the obtained ash and double distilled water, settling for 5-8 hours, taking supernatant fluid, and filtering to obtain filtrate which is plant ash buffer solution; the mass volume ratio of the ash to the double distilled water is 5-10 g: 100 ml.
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