CN108156986A - External source epiphysin is to apple dwarf from the application of rootstock seedling ozone stress relieving effect - Google Patents
External source epiphysin is to apple dwarf from the application of rootstock seedling ozone stress relieving effect Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a kind of external source epiphysin to apple dwarf from the application of rootstock seedling ozone stress relieving effect.The present invention provides epiphysins to alleviate apple dwarf from the application in the damage of rootstock ozone stress.The present invention specifies concentration threshold and physio-biochemical characteristics of the ozone to apple dwarf from rootstock M9T337 seedling injuries, it was found that alleviates epiphysin and its processing mode and appropriate concentration range of the apple dwarf from rootstock M9T337 seedling ozone stress.The present invention is scientific evaluation surface layer O3Influence and protection of the concentration variation to plant growth provide theoretical foundation.Present invention can apply to scientific evaluation surface layer O3Influence and protection and the selection and breeding of ozone-resistant dwarfing rootstock of the concentration variation to plant growth, have major application prospect in breeding plant.
Description
Technical field
The present invention relates to a kind of external source epiphysin to apple dwarf from the application of rootstock seedling ozone stress relieving effect.
Background technology
Ozone (O3) it is a kind of slightly special bad smell gas azury, it is present in stratosphere and troposphere air.
Ozone in stratosphere is " umbrella of life on earth ", because ozone can absorb the animals and plants of UV protection earth surface
It does not come to harm.And the existence of tropospheric ozone then serious threat animals and plants, the especially surface layer apart from ground 1-2km are smelly
The increase of oxygen content produces serious harm to tellurian life.Recently as China's industry and economic rapid
Development, industrial waste gas, vehicle exhaust discharge capacity increasingly increase, atmospheric environment is damaged, leads to tropospheric ozone
Concentration increases
According to the address prediction of IPCC, the ozone mean concentration of end of this century Northern Hemisphere continent summer air is likely to be breached
70nL·L-1More than.China's surface layer ozone concentration increases comparatively fast, and average monitored concentration has reached 50-60n LL-1.According to
74 urban air-qualities monitoring situation report that Environmental Protection Department in monthly, 2013 is issued, China's atmosphere pollution are mainly
PM2.5 and ozone, but as the adjoint temperature raising of seasonal law and illumination are strengthened, ozone substitution PM2.5 becomes many areas
Primary pollutant.Pearl River Delta area, which amounts to, has six months mainly based on ozone pollution；Yangtze River Delta Area ozone pollution is held
The continuous time is slightly shorter compared with Pearl River Delta area, concentrates on May to September, 5 months altogether；Beijing-tianjin-hebei Region whole year is mainly polluted with PM2.5
Based on, but have ozone exceeded phenomenon May to September, wherein the exceeded number of days of August part ozone accounts for the 48.3% of total exceeded number of days, surpasses
The 41.4% of PM2.5 is crossed, ozone pollution at present has become the primary pollution in China.
The U.S., Europe and other countries start to grind ozone deplation about ozone in the 80s and 90s in last century
Study carefully.Some researches show that high-concentrated ozone can reduce wheat, soybean yield, influence the growth of ornamental plant.High concentration
Ozone is entered by stomata in plant, can be generated visible injury to the blade of plant first, can generally be shown as chlorosis, burn
Burning, withered phenomenon and then the photosynthetical system for destroying plant, accelerate the degradation of chlorophyll, reduce photosynthetic efficiency, reduce the sight of plant
Reward property.Ozone stress can also destroy the antioxidant system of plant, reduce the activity of SOD, POD and CAT, and aggravate film fat peroxide
Change, seriously affect plant growth.Apple dwarf is widely used from rootstock M9T337 as a kind of in Malus cultivation
Stock, ozone stress can seriously affect growth, and then influence yield, quality and the ornamental character of ornamental plant of apple.
Invention content
Apple dwarf from rootstock seedling ozone stress is alleviated the object of the present invention is to provide a kind of external source epiphysin and is imitated
The application answered.
The present invention provides epiphysins to alleviate apple dwarf from the application in the damage of rootstock ozone stress.
The application of present invention protection epiphysin, at least one of following (a1)-(a4)：
(a1) increase apple dwarf activities of antioxidant enzymes from rootstock under ozone stress；
(a2) inhibit the generation of apple dwarf active oxygen from rootstock under ozone stress；
(a3) apple dwarf active o content from rootstock under ozone stress is reduced；
(a4) apple dwarf is improved from rootstock ozone stress resistance.
The present invention also protects a kind of product, and active constituent is epiphysin；The purposes of the product is following (b1)-(b5)
At least one of：
(b1) alleviate apple dwarf to damage from rootstock ozone stress；
(b2) increase apple dwarf activities of antioxidant enzymes from rootstock under ozone stress；
(b3) inhibit the generation of apple dwarf active oxygen from rootstock under ozone stress；
(b4) apple dwarf active o content from rootstock under ozone stress is reduced；
(b5) apple dwarf is improved from rootstock ozone stress resistance.
The present invention also application of the protection epiphysin in product is produced；The purposes of the product is in following (b1)-(b5)
At least one：
(b1) alleviate apple dwarf to damage from rootstock ozone stress；
(b2) increase apple dwarf activities of antioxidant enzymes from rootstock under ozone stress；
(b3) inhibit the generation of apple dwarf active oxygen from rootstock under ozone stress；
(b4) apple dwarf active o content from rootstock under ozone stress is reduced；
(b5) apple dwarf is improved from rootstock ozone stress resistance.
The present invention also protects a kind of breeding method of apple dwarf from rootstock, includes the following steps：Using a concentration of 50-
100μmol·L-1Epiphysin to the apple dwarf from rootstock carry out pouring root.
Described method includes following steps：Using a concentration of 50 μm of olL-1Epiphysin to the apple dwarf from root anvil
Wood carries out pouring root.
Described method includes following steps：Using a concentration of 100 μm of olL-1Epiphysin to the apple dwarf from root
Stock carries out pouring root.
The present invention also protects a kind of breeding method of apple dwarf from rootstock, includes the following steps：Using a concentration of 1-
10μmol·L-1Epiphysin to the apple dwarf from rootstock carry out foliage-spray.
Described method includes following steps：Using a concentration of 1 μm of olL-1Epiphysin to the apple dwarf from root anvil
Wood carries out foliage-spray.
Described method includes following steps：Using a concentration of 10 μm of olL-1Epiphysin to the apple dwarf from root anvil
Wood carries out foliage-spray.
The present invention also protects a kind of method alleviated apple dwarf and damaged from rootstock ozone stress, includes the following steps：
Using a concentration of 50-100 μm of olL-1Epiphysin to the apple dwarf from rootstock carry out pouring root.
Described method includes following steps：Using a concentration of 50 μm of olL-1Epiphysin to the apple dwarf from root anvil
Wood carries out pouring root.
Described method includes following steps：Using a concentration of 100 μm of olL-1Epiphysin to the apple dwarf from root
Stock carries out pouring root.
The present invention also protects a kind of method alleviated apple dwarf and damaged from rootstock ozone stress, includes the following steps：
Using a concentration of 1-10 μm of olL-1Epiphysin to the apple dwarf from rootstock carry out foliage-spray.
Described method includes following steps：Using a concentration of 1 μm of olL-1Epiphysin to the apple dwarf from root anvil
Wood carries out foliage-spray.
Described method includes following steps：Using a concentration of 10 μm of olL-1Epiphysin to the apple dwarf from root anvil
Wood carries out foliage-spray.
In any description above method, the apple dwarf is from the apple dwarf of rootstock concretely Seedling Stage from root anvil
Wood.
The present invention also protection epiphysin is in ozone-resistant apple dwarf from the application in rootstock selection and breeding.
The present invention also protection any description above method is in ozone-resistant apple dwarf from the application in rootstock selection and breeding.
Any description above apple dwarf from rootstock concretely apple dwarf from rootstock M9T337.
The present invention specifies concentration threshold and Physiology and biochemistry of the ozone to apple dwarf from rootstock M9T337 seedling injuries
Characteristic, it was found that alleviate apple dwarf from the epiphysin and its processing mode of rootstock M9T337 seedling ozone stress and suitable for dense
Spend range.The present invention is scientific evaluation surface layer O3Influence and protection of the concentration variation to plant growth provide theoretical foundation.
Present invention can apply to scientific evaluation surface layer O3Influence and protection and ozone-resistant dwarfing stock of the concentration variation to plant growth
The selection and breeding of wood have major application prospect in breeding plant.
Description of the drawings
Fig. 1 is the injury apparent to M9T337 blades of different ozone concentrations.
Fig. 2 is influence of the different ozone concentrations to M9T337 MDA concentration in leaves.
Fig. 3 is influence of the different ozone concentrations to SOD activity in M9T337 blades.
Fig. 4 is influence of the different ozone concentrations to POD activity in M9T337 blades.
Fig. 5 is influence of the different ozone concentrations to CAT activity in M9T337 blades.
Fig. 6 is influence of the different ozone concentrations to soluble protein content in M9T337 blades.
Fig. 7 be various concentration MT root irrigations to the variation of M9T337 blades Visible injury under ozone stress (young leaves liken to
Ripe leaf amplification is twice).
Fig. 8 is influence of the various concentration MT root irrigations to SOD activity in M9T337 mature leafs under ozone stress.
Fig. 9 is influence of the various concentration MT root irrigations to SOD activity in M9T337 young leaves blade under ozone stress.
Figure 10 is influence of the various concentration MT root irrigations to POD in M9T337 mature leafs under ozone stress.
Figure 11 is influence of the various concentration MT root irrigations to POD in M9T337 young leaves blade under ozone stress.
Figure 12 is influence of the various concentration MT root irrigations to CAT activity in M9T337 mature leafs under ozone stress.
Figure 13 is influence of the various concentration MT root irrigations to CAT activity in M9T337 young leaves blade under ozone stress.
Figure 14 is influence of the various concentration MT root irrigations to MDA contents in M9T337 mature leafs under ozone stress.
Figure 15 is influence of the various concentration MT root irrigations to M9T337 young leaves MDA concentration in leaves under ozone stress.
Figure 16 is various concentration MT root irrigations to soluble protein content in M9T337 mature leafs under ozone stress
It influences.
Figure 17 is various concentration MT root irrigations to soluble protein content in M9T337 young leaves blade under ozone stress
It influences.
Figure 18 is influence of the various concentration MT root irrigations to M9T337 mature leafs APx activity under ozone stress.
Figure 19 is influence of the various concentration MT root irrigations to M9T337 young leaves blade APx activity under ozone stress.
Figure 20 is influence of the various concentration MT root irrigations to AsA contents in M9T337 mature leafs under ozone stress.
Figure 21 is influence of the various concentration MT root irrigations to AsA contents in M9T337 young leaves blade under ozone stress.
Figure 22 is influence of the various concentration MT root irrigations to GSH contents in M9T337 mature leafs under ozone stress.
Figure 23 is influence of the various concentration MT root irrigations to GSH contents in M9T337 young leaves blade under ozone stress.
For variation of the various concentration MT foliar sprays to M9T337 blades Visible injury under ozone stress, (young leaves compares climax leaves to Figure 24
Amplification is twice).
Figure 25 is influences of the foliar spray various concentration MT to SOD activity in M9T337 mature leafs under ozone stress.
Figure 26 is influences of the foliar spray various concentration MT to SOD activity in M9T337 young leaves blade under ozone stress.
Figure 27 is influences of the foliar spray various concentration MT to POD activity in M9T337 mature leafs under ozone stress.
Figure 28 is influences of the foliar spray various concentration MT to POD activity in M9T337 young leaves blade under ozone stress.
Figure 29 is influences of the foliar spray various concentration MT to CAT activity in M9T337 climax leaves blade under ozone stress.
Figure 30 is influences of the foliar spray various concentration MT to CAT activity in M9T337 young leaves blade under ozone stress.
Figure 31 is influences of the foliar spray various concentration MT to MDA contents in M9T337 mature leafs under ozone stress.
Figure 32 is influences of the foliar spray various concentration MT to M9T337 young leaves MDA concentration in leaves under ozone stress.
Figure 33 is influences of the foliar spray various concentration MT to soluble protein content in M9T337 mature leafs under ozone stress.
Figure 34 is influences of the foliar spray various concentration MT to soluble protein content in M9T337 young leaves blade under ozone stress.
Figure 35 is influences of the foliar spray various concentration MT to APx activity in M9T337 mature leafs under ozone stress.
Figure 36 is influences of the foliar spray various concentration MT to APx activity in M9T337 young leaves blade under ozone stress.
Figure 37 is influences of the foliar spray various concentration MT to AsA contents in M9T337 mature leafs under ozone stress.
Figure 38 is influences of the foliar spray various concentration MT to AsA contents in M9T337 young leaves blade under ozone stress.
Figure 39 is influences of the foliar spray various concentration MT to GSH contents in M9T337 mature leafs under ozone stress.
Figure 40 is influences of the foliar spray various concentration MT to GSH contents in M9T337 young leaves blade under ozone stress.
Specific embodiment
Following embodiment facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is conventional method unless otherwise specified.Test material used in following embodiments is certainly unless otherwise specified
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Using Dwarf rootstocks wood M9T337 as test material in embodiment.Dwarf rootstocks wood M9T337 (also known as apples
Fruit Dwarf rootstocks wood M9T337)：Bibliography：Apples Dwarf Stocks M9T337 [J] northwest gardening (fruit tree), 2014, (05):
53.；The public can obtain from Shandong Agricultural University.
Ozone treatment in embodiment is carried out using ozone fumigation system.
Ozone fumigation system：Ozone fumigation system is respectively by open top type air chamber (OTC), air supply system (oxygen tank), ozone
Occur and concentration control system (ozone generator and flowmeter), ozone concentration monitoring system (real-time ozone concentration monitor) four
Part forms.Bibliography：Geng Chunmei, Yang Wen, Yin Baohui wait fields original position top-open type ozone fumigation system research [J] rings
Border scientific research, 2011,24 (6):593-600.
The workflow of ozone fumigation system is as follows：The oxygen exported by oxygen tank enters ozone generator (WJ-HY5, Ji
It is output in pipeline after Nan Sankang), is then entered top-open type under the action of axial flow blower (SF2-2 types, Shenyang Shen Li boards)
In gas chamber (OTC).The indoor ozone concentration monitor of gas (DR70C- ozone-types, Shenzhen Wo Saite) can be monitored in gas chamber in real time
Ozone concentration, and in data transmission to computer for observing, preserve.Ozone generator can be adjusted by spinner flowmeter
Oxygen feeding amount, so as to control the indoor ozone concentration of gas.
Epiphysin (MT)：It buys in sigma companies, article No. M5250.
The ozone treatment M9T337 and physiological index determining of embodiment 1, various concentration
First, test process
Using various concentration (60 ± 10nlL-1、80±10nl·L-1、100±10nl·L-1With 120 ± 10nlL-1)
Ozone Dwarf rootstocks wood M9T337 seedling is handled, setting control group (CK) ozone concentration be 20 ± 10nlL-1,
Experiment carries out in ozone fumigation system, coprocessing 3 hours.
2nd, physiological index determining
Phenotypic Observation and physical signs (leaf are carried out to each group Dwarf rootstocks wood M9T337 seedling leaves of step 1 processing
Chlorophyll contents, superoxide dismutase activity, peroxidase activity, catalase activity, mda content and solubility
Protein content) it measures.
Chlorophyll content is measured using acetone method；Superoxide dismutase (SOD) activity is surveyed using superoxide dismutase
It is fixed；The measure of peroxidase (POD) activity is measured using guaiacol method；Catalase (CAT) activity uses ultraviolet point
Light photometry measures；Malonaldehyde (MDA) content uses thiobarbituricacidα- (TBA) determination of color；Soluble protein content is adopted
It is measured with dying method with coomassie brilliant blue.
3rd, result
Each group Phenotypic Observation the result is shown in Figure 1.Each group measuring chlorophyll content the results are shown in Table 1.Each group MDA assay results
See Fig. 2.Each group SOD determination of activity results are shown in Fig. 3.Each group POD Activity determination results are shown in Fig. 4.Each group CAT Activity determination results are shown in
Fig. 5.Each group soluble protein content measurement result is shown in Fig. 6.
The above results show the raising with ozone concentration, and the Visible injury of M9T337 blades constantly increases；Chlorophyll
Content continuously decreases；SOD (superoxide dismutase), the activity of CAT (catalase) and soluble protein content are in first to rise
Downward trend after height, and the activity of POD (peroxidase) and the content of MDA (malonaldehyde) gradually rise.Comprehensive analysis
Indices show that M9T337 seedling can endure the short time (the ﹤ 80nlL of (﹤ 3h) interior low concentration-1) ozone stress, when smelly
Oxygen concentration is more than 80nlL-1When can generate serious injury to M9T337.Therefore this experiment uses 100nlL-1Ozone it is dense
Degree carries out next step experiment.
Influence of the different ozone concentrations of table 1 to M9T337 chlorophyll content in leaf blades
The suitable epiphysin concentration and physiological and biochemical index of embodiment 2, apple rootstock M9T337 seedling ozone-resistant stress
First, test process
Pouring root is respectively adopted and two methods of foliage-spray are tested.
1st, pouring root：The the 3rd, 6,9,12,15 day daily 17:30-18:30 pairs of Dwarf rootstocks wood M9T337 seedling roots
It pours and contains various concentration epiphysin (0.1 μm of olL-1、1μmol·L-1、10μmol·L-1、50μmol·L-1With 100 μ
mol·L-1) 1/2Hoagland nutrient solutions, control group (CK), which pours, does not contain the 1/2Hoagland nutrient solutions of epiphysin, often
A concentration handles 20 seedling；(labeled as T0) was first sampled for the first time at the 18th day, after blade sampling for the first time, to each group seedling
Carry out ozone treatment (ozone concentration 100nlL-1), common 3h is handled, 0h, 8h and 16h progress the after ozone treatment
2nd, three and four sub-sampling (being respectively labeled as T1, T2 and T3).
It is above-mentioned not take young leaves and functional leaf per the sub-sampling time-division.
2nd, foliage-spray：The the 2nd, 4,6,8,10 day daily 17:30-18:30 pairs of Dwarf rootstocks wood M9T337 seedling
Foliage-spray contains various concentration epiphysin (0.1 μm of olL-1、1μmol·L-1、10μmol·L-1、50μmol·L-1With 100 μ
mol·L-1) water, Application is dripped with blade to be advisable, and control group (CK), which pours, does not contain the water of epiphysin, at each concentration
Manage 20 seedling；It was first sampled (labeled as T0) for the first time at the 12nd day, after blade sampling for the first time, ozone is carried out to each group seedling
Handle (ozone concentration 100nlL-1), handle common 3h, after ozone treatment 0h, 8h and 16h carry out second and third and
Four sub-samplings (are respectively labeled as T1, T2 and T3).
2nd, physiological index determining
Phenotypic Observation and physical signs (leaf are carried out to each group Dwarf rootstocks wood M9T337 seedling leaves of step 1 processing
Chlorophyll contents, superoxide dismutase activity, peroxidase activity, catalase activity, mda content, soluble egg
Bai Hanliang, ascorbate peroxidase enzyme, ascorbic acid content and glutathione content) it measures.
Chlorophyll content is measured using acetone method；Superoxide dismutase (SOD) activity is surveyed using superoxide dismutase
It is fixed；The measure of peroxidase (POD) activity is measured using guaiacol method；Catalase (CAT) activity uses ultraviolet point
Light photometry measures；Malonaldehyde (MDA) content uses thiobarbituricacidα- (TBA) determination of color；Soluble protein content is adopted
It is measured with dying method with coomassie brilliant blue；Ascorbate peroxidase enzyme (APx) determination of activity is with reference to Nakano＆AsAds (1981)
Method (Nakano Y, Asada K.Hydrogen Peroxide is Scavenged by Ascorbate-specific
Peroxidase in Spinach Chloroplasts[J].Plant&Cell Physiology,1981,22(5):867-
880.)；Ascorbic acid (AsA) content is measured using spectrophotometer method；Glutathione (GSH) content refers to Griffith
(1980) method (Griffith O W.Determination of glutathione and glutathione
disulfide using glutathione reductase and 2-vinylpyridine.[J].Analytical
Biochemistry,1980,106(1):207.)。
3rd, measurement result
Blade (T3) Phenotypic Observation result that pouring root mode is handled is shown in Fig. 7.Pouring root mode handles the leaf in after ripening blade
Chlorophyll contents statistical result is shown in Table 2.Chlorophyll content statistical result after pouring root mode is handled in young leaves blade is shown in Table 3.Pouring root
Mode handles SOD determinations of activity result in after ripening blade and sees Fig. 8.SOD determinations of activity in young leaves blade after pouring root mode is handled
As a result see Fig. 9.Pouring root mode handles POD determinations of activity the result is shown in Figure 10 in after ripening blade.Young leaves leaf after pouring root mode is handled
POD determinations of activity the result is shown in Figure 11 in piece.Pouring root mode handles CAT determinations of activity the result is shown in Figure 12 in after ripening blade.Pouring root
CAT determinations of activity the result is shown in Figure 13 in young leaves blade after mode is handled.Pouring root mode handles after ripening MDA concentration in leaves measure
The result is shown in Figure 14.Young leaves MDA concentration in leaves measurement result is shown in Figure 15 after pouring root mode is handled.Pouring root mode handles after ripening leaf
Soluble protein content measurement result is shown in Figure 16 in piece.Soluble protein content measures knot in young leaves blade after pouring root mode is handled
Fruit sees Figure 17.Pouring root mode handles APx determinations of activity the result is shown in Figure 18 in after ripening blade.Young leaves blade after pouring root mode is handled
Middle APx determinations of activity the result is shown in Figure 19.Pouring root mode handles AsA assays result in after ripening blade and sees Figure 20.Pouring root side
AsA assay results are shown in Figure 21 in young leaves blade after formula processing.Pouring root mode handles GSH assays knot in after ripening blade
Fruit sees Figure 22.GSH assays result is shown in Figure 23 in young leaves blade after pouring root mode is handled.
Blade (T3) Phenotypic Observation result that foliage-spray mode is handled is shown in Figure 24.Foliage-spray mode handles after ripening leaf
Chlorophyll content statistical result in piece is shown in Table 4.Chlorophyll content statistics knot after foliage-spray mode is handled in young leaves blade
Fruit is shown in Table 5.Foliage-spray mode handles SOD determinations of activity result in after ripening blade and sees Figure 25.After foliage-spray mode is handled
SOD determinations of activity result is shown in Figure 26 in young leaves blade.Foliage-spray mode handles POD determinations of activity result in after ripening blade and sees
Figure 27.POD determinations of activity result is shown in Figure 28 in young leaves blade after foliage-spray mode is handled.Foliage-spray mode handles after ripening
CAT determinations of activity result is shown in Figure 29 in blade.CAT determinations of activity result is shown in figure in young leaves blade after foliage-spray mode is handled
30.Foliage-spray mode handles after ripening MDA concentration in leaves measurement result and sees Figure 31.Young leaves leaf after foliage-spray mode is handled
MDA assays result is shown in Figure 32 in piece.Foliage-spray mode handles soluble protein content measurement result in after ripening blade
See Figure 33.Soluble protein content measurement result is shown in Figure 34 in young leaves blade after foliage-spray mode is handled.Foliage-spray mode
APx determination of activity results are shown in Figure 35 in processing after ripening blade.APx determinations of activity in young leaves blade after foliage-spray mode is handled
As a result see Figure 36.Foliage-spray mode handles AsA assays result in after ripening blade and sees Figure 37.Foliage-spray mode is handled
AsA assay results are shown in Figure 38 in young leaves blade afterwards.Foliage-spray mode handles GSH assays result in after ripening blade
See Figure 39.GSH assays result is shown in Figure 40 in young leaves blade after foliage-spray mode is handled.
2 various concentration MT root irrigations of table are to M9T337 climax leaves Determination of Chlorophyll content under ozone stress
Chlorophyll content statistical result after 3 pouring root mode of table is handled in young leaves blade
The processing of 4 various concentration MT foliar sprays of table is to M9T337 climax leaves Determination of Chlorophyll content under ozone stress
The processing of 5 various concentration MT foliar sprays of table is to M9T337 climax leaves Determination of Chlorophyll content under ozone stress
It is that pouring root mode is handled the result shows that, with the increase of epiphysin concentration, chlorophyll content, activities of antioxidant enzymes
(SOD, POD, CAT, APx) and GSH contents are also gradually increased, in a concentration of 100 μm of olL of epiphysin-1When reach highest.
At T3 (16h after ozone stress), pouring root applies 100 μm of olL-1The Chlorophyll content of epiphysin, climax leaves and young leaves
16.76% and 13% are increased respectively relative to control；71.69% and 61.07% (P has been respectively increased in SOD activity<0.0.1)；
38.1% and 23.22% (P has been respectively increased in POD activity<0.0.1)；28.05% and 45.46% (P is respectively increased in CAT activity<
0.0.1)；66.23% and 55.32% has been respectively increased in APx activity；124.9% and 127.77% has been respectively increased in GSH contents.
Soluble protein and AsA contents, the increase with epiphysin concentration is in the trend reduced afterwards is first increased, in epiphysin a concentration of 50
μmol·L-1When reach highest, hereafter reduce.In addition M9T337 seedling leaf under ozone stress can be significantly reduced by applying epiphysin
The content of MDA in piece, with the raising of epiphysin concentration, raised trend after first reducing is presented in MDA contents, and pouring root application is taken off black
Element, as a concentration of 50 μm of olL-1When, content is minimum.
It is that foliage-spray mode is handled the result shows that, the application of variation tendency and pouring root is consistent, with epiphysin concentration
Increase its indices (except MDA) in increased trend, but epiphysin chlorophyll content, the activities of antioxidant enzymes of foliage-spray
(SOD, POD, CAT, APx) and GSH contents are in a concentration of 10 μm of olL of epiphysin-1When reach highest.Soluble protein and
AsA contents are in a concentration of 1 μm of olL of epiphysin-1When reach highest.When epiphysin concentration continues to increase the meeting of its indices gradually
It reduces, when epiphysin concentration reaches 100 μm of olL-1When even can be less than control.During foliage-spray epiphysin, MDA contents with
The raising of epiphysin concentration and reduce, a concentration of 1 μm of olL of epiphysin when MDA contents are minimum-1。
In conclusion external source epiphysin can effectively improve M9T337 seedling photosynthesis under ozone stress, enhance plant
Interior activities of antioxidant enzymes, promotes the level of antioxidant, the generation of inhibitory activity oxygen or removes the active oxygen generated, alleviates
The adverse reaction that ozone comes vegetational zone enhances resistance.Optium concentration when epiphysin pouring root is applied is 50-100 μm of ol
L-1, the optium concentration that epiphysin fills blade spraying is 1-10 μm of olL-1。
Claims (10)
1. epiphysin is alleviating apple dwarf from the application in the damage of rootstock ozone stress.
2. the application of epiphysin, at least one of following (a1)-(a4)：
(a1) increase apple dwarf activities of antioxidant enzymes from rootstock under ozone stress；
(a2) inhibit the generation of apple dwarf active oxygen from rootstock under ozone stress；
(a3) apple dwarf active o content from rootstock under ozone stress is reduced；
(a4) apple dwarf is improved from rootstock ozone stress resistance.
3. a kind of product, active constituent is epiphysin；The purposes of the product is at least one of following (b1)-(b5)：
(b1) alleviate apple dwarf to damage from rootstock ozone stress；
(b2) increase apple dwarf activities of antioxidant enzymes from rootstock under ozone stress；
(b3) inhibit the generation of apple dwarf active oxygen from rootstock under ozone stress；
(b4) apple dwarf active o content from rootstock under ozone stress is reduced；
(b5) apple dwarf is improved from rootstock ozone stress resistance.
4. application of the epiphysin in product is produced；The purposes of the product is at least one of following (b1)-(b5)：
(b1) alleviate apple dwarf to damage from rootstock ozone stress；
(b2) increase apple dwarf activities of antioxidant enzymes from rootstock under ozone stress；
(b3) inhibit the generation of apple dwarf active oxygen from rootstock under ozone stress；
(b4) apple dwarf active o content from rootstock under ozone stress is reduced；
(b5) apple dwarf is improved from rootstock ozone stress resistance.
5. a kind of apple dwarf includes the following steps from the breeding method of rootstock：Using a concentration of 50-100 μm of olL-1's
Epiphysin carries out pouring root to the apple dwarf from rootstock.
6. a kind of apple dwarf includes the following steps from the breeding method of rootstock：Using a concentration of 1-10 μm of olL-1Take off
Melanocyte carries out foliage-spray to the apple dwarf from rootstock.
7. a kind of method alleviated apple dwarf and damaged from rootstock ozone stress, includes the following steps：Using a concentration of 50-
100μmol·L-1Epiphysin to the apple dwarf from rootstock carry out pouring root.
8. a kind of method alleviated apple dwarf and damaged from rootstock ozone stress, includes the following steps：Using a concentration of 1-10 μ
mol·L-1Epiphysin to the apple dwarf from rootstock carry out foliage-spray.
9. epiphysin is in ozone-resistant apple dwarf from the application in rootstock selection and breeding.
10. any methods of claim 5-8 are in ozone-resistant apple dwarf from the application in rootstock selection and breeding.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116018955A (en) * | 2022-11-28 | 2023-04-28 | 河北农业大学 | Application of melatonin in relieving growth inhibition of phlorizin on plants |
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| CN1300277A (en) * | 1998-05-12 | 2001-06-20 | 阿迪尔公司 | Novel substituted cyclic compounds, preparation method and pharmaceutical compositions containing them |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1300277A (en) * | 1998-05-12 | 2001-06-20 | 阿迪尔公司 | Novel substituted cyclic compounds, preparation method and pharmaceutical compositions containing them |
Non-Patent Citations (1)
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| 耿庆伟等: "外源褪黑素对臭氧胁迫下‘赤霞珠’葡萄叶片光合作用的影响", 《园艺学报》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116018955A (en) * | 2022-11-28 | 2023-04-28 | 河北农业大学 | Application of melatonin in relieving growth inhibition of phlorizin on plants |
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