CN108148923A - The molecular detection primer and its detection method of potato plant tikka class disease alternaria solani sorauer pathogen - Google Patents
The molecular detection primer and its detection method of potato plant tikka class disease alternaria solani sorauer pathogen Download PDFInfo
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- CN108148923A CN108148923A CN201810154052.2A CN201810154052A CN108148923A CN 108148923 A CN108148923 A CN 108148923A CN 201810154052 A CN201810154052 A CN 201810154052A CN 108148923 A CN108148923 A CN 108148923A
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Abstract
The molecular detection primer and its detection method of potato plant tikka class disease alternaria solani sorauer pathogen, it is related to potato detection field, the present invention is directed to potato to solve current traditional biological method, it is difficult the primer sequence of the invention such as sequence table Seq ID No species separation Alternaria is various the problem of：1 shown and Seq ID No：Shown in 2, PCR detections are carried out using above-mentioned primer pair sample to be tested, method of the invention can be quick, accurately identifies sporulation (Alternaria solani), and detection sensitivity is 10pg/ μ L.
Description
Technical field
The present invention relates to potato detection fields, specially potato plant tikka class disease Alternaria (alternaria solani sorauer
Bacterium (Alternaria solani)) pathogen molecular detection primer and its detection method.
Background technology
The gross area and total output of China potato leap to the first in the world, but per unit area yield is in reduced levels, various
It is one of the main reason for causing the potato underproduction that disease, which takes place frequently,.In potato disease, with fungal disease most species, and have
Propagated, prevention and control are difficult, and some fungies can also morph, and serious threat is caused to potato production.Alternaria is to cause
The important fungal pathogen of one kind of potato plant and leaf spot lesion.According to Zheng etc. (2015), potatoes plant is infected
Alternaria belong to cause of disease there are mainly three types of：Alternaria solani sorauer (Alternaria solani), rod method (Alternaria
Alternata) and Alternaria tenuissima (Alternaria tenuissima), these three cause of diseases cause potato early epidemic respectively
Disease, diplostomiasis and black spot can lead to the symptoms such as plant leaf is downright bad, plant withers, stem tuber can be also infected when serious, so as to
Cause the underproduction.The Heilongjiang Province potato production base important as the whole nation, Alternaria belong to cause of disease on potato plant
Species distributing situation also it is fresh for report, investigation in recent years Heilongjiang Province potato Alternaria belong to cause of disease type, for
All kinds of diseases caused by targetedly prevention Alternaria belongs to cause of disease are of great significance.
The different microspecies of many Alternarias have all been classified, and are shown in physiology, form, the base for planting interior or inter-species
Cause and virulence variation.This may be that the function served as bridge being connected with each other due to fungal mycelium can promote nutrition, moisture and signal
The mutual transmission of molecule.Many experimental methods as Virulent Analysis, vegetative compatibility group and molecule biochemical analysis are all used for examining
Variation in Alternaria inter-species and kind, the interior variation of Alternaria inter-species, kind can be with let us more in the probe season of growth
Epidemiology and management cause of disease between different year are understood well.These three diseases of target, diplostomiasis and black spot exist
Symptom on plant is extremely similar, and scab can be all formed on blade, and spot shape is also very similar with color, field this three
Kind cause of disease is under normal conditions mixed infection, therefore is difficult to judge these three diseases by estimating its symptom, further, since many
Morphological differences is smaller under the microscope for Alternaria microspecies, so traditional biological method is difficult each in species separation Alternaria
Microspecies.
Invention content
The present invention is in order to solve current traditional biological method for potato, it is difficult to various in species separation Alternaria
The problem of, the problem of being especially to discriminate between alternaria solani sorauer (Alternaria solani).And provide potato plant tikka class disease
The molecular detection primer and its detection method of evil alternaria solani sorauer pathogen.
The primer sequence such as sequence table Seq ID No of the present invention：1 shown and Seq ID No：Shown in 2.
The molecular detecting method of the potato plant tikka class disease alternaria solani sorauer pathogen of the present invention, it is according to following
What step carried out：
First, to extract potato gene group DNA to be detected as template, using the primer of claim 1, PCR expansions are carried out
Increase；
2nd, PCR product is taken to carry out detected through gel electrophoresis, if being capable of detecting when the segment of 270bp sizes, can determine whether out institute
There are alternaria solani sorauer pathogens in the potato of detection.
The present invention has comprising following with effect：
The present invention is using design according to I gene orders of Alternaria ribosomes the Internal Transcribed Spacer ITS and Histone 3
(Histone3) Alternaria of gene conserved sequence design belongs to (especially sporulation (Alternaria solani))
Primer carries out PCR amplification, establishes a set of PCR identification technologies that can fast and accurately identify Alternaria and belong to cause of disease, with reference to
Traditional biological identification method, investigation and analysis Heilongjiang Province potato tikka class disease Alternaria belong to the composition of cause of disease and divide
Cloth situation, to provide foundation targetedly to prevent such disease in production.
The method of the present invention can be quick, accurately identifies sporulation (Alternaria solani), and detection is sensitive
It spends for 10pg/ μ L.
Description of the drawings
Fig. 1 is the electrophoretogram that the susceptible potato leaf of PCR detections is carried out using primer WASF/WASR；Wherein, M:Standard scores
Son amount DL600；1-10:Potato tikka class disease DNA；11:Negative control (sterile water)；12:Sporulation
(Alternaria solani) positive strain DNA；
Fig. 2 is primer WASF/WASR specificity verification electrophoretograms；Wherein, M:Standard molecular weight DL600；1-8:Respectively
A.solani bacterial strains DNA, A.alternata bacterial strain DNA, A.tenuissima bacterial strain DNA, late blight bacterial strain DNA, Streptomyces scabies
Strain DNA, bacterial wilt bacterial strain DNA, balck shank bacterial strain DNA, tar spot bacterial strain DNA；9:A.solani positive strains DNA；10:It is negative
It compares (sterile water)；
Fig. 3 verifies electrophoretogram for primer WASF/WASR sensitivity；Wherein, M:Standard molecular weight DL600；1:Negative control
(sterile water)；2-7:The pure bacterial strain DNA of respectively A.solani are diluted to 100ng/ μ l, 50ng/ μ l, 10ng/ μ l, 1ng/ μ respectively
L, 100pg/ μ l, 10pg/ μ l.
Specific embodiment
Specific embodiment one：The primer sequence of present embodiment such as sequence table Seq ID No：1 shown and Seq ID
No：Shown in 2.
Specific embodiment two：The molecule inspection of the potato plant tikka class disease alternaria solani sorauer pathogen of present embodiment
Survey method, it is followed the steps below：
First, to extract potato gene group DNA to be detected as template, using the primer of claim 1, PCR expansions are carried out
Increase；
2nd, PCR product is taken to carry out detected through gel electrophoresis, if being capable of detecting when the segment of 270bp sizes, can determine whether out institute
There are alternaria solani sorauer pathogens in the potato of detection.
Specific embodiment three：Present embodiment is unlike specific embodiment two：The alternaria solani sorauer cause of disease
Bacterium is sporulation (Alternaria solani).It is other to be identical with embodiment two.
Specific embodiment four：Present embodiment is unlike specific embodiment two：The PCR reaction systems are
20 μ L are as follows：
PCR amplification condition is：5 DEG C of pre-degeneration 1min, 94 DEG C of denaturation 30sec, 54 DEG C of annealing 30sec, 72 DEG C extend
30sec, 72 DEG C extend 8min eventually, totally 29 cycles.
It is other to be identical with embodiment two.
Specific embodiment five：Present embodiment is unlike specific embodiment two：It, will after PCR is detected successfully
PCR product after purification, is sequenced, and carries out Blast comparisons and homology analysis.It is other to be identical with embodiment two.
The content of present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several specific embodiments
Contract sample can also realize the purpose of invention.
Beneficial effects of the present invention are verified by following embodiment：
Embodiment
1st, strains tested
2015-2016 respectively from Heilongjiang Academy of Agricultural Sciences democracy garden, Harbin, Hulan area, Jiamusi, Hegang,
The ground such as Keshan acquisition potato Alternaria belongs to tikka class disease blade, and pathogen is isolated and purified according to conventional organization partition method
It is spare.
2nd, the separation of pure bacterial strain
Early blight after isolating and purifying is observed under the microscope, with single-cell technic combination identification method, unit cell point
Sporulation (Alternaria solani) pathogen studied from technology separation, obtains pure bacterial strain.
3rd, sporulation (Alternaria solani) scab blade total DNA that potato Alternaria belongs to carries
It takes
Carry out DNA's using the TIANGEN Plant Genomes extracts kit of TIANGEN Biotech (Beijing) Co., Ltd.
Extraction is extracted according to the following operating procedure of kit：
(1) mycelia or disease leaf texture 100mg are taken, liquid nitrogen is added in and is fully ground.Add in 400 μ L buffer solutions LP1 and 6ul
RNaseA (10mg/ml), vortex oscillation 1min are placed at room temperature for 10min.
(2) 130 μ L buffer solutions LP2, abundant mixing, vortex oscillation 1min are added in.
(3) 12000rpm centrifuges 5min, supernatant is moved in new centrifuge tube.
(4) the buffer solution LP3 (such as the supernatant of 500 μ L adds in the buffer solution LP3 of 750 μ L) of 1.5 times of volumes is added in, immediately
Fully oscillation mixing 15sec.
(5) previous step acquired solution and flocculent deposit are all added in an adsorption column, 12000rpm centrifugation 30sec,
Fall waste liquid, adsorption column is put back into collecting pipe.
(6) 600 μ L bleaching liquid PW, 12000rpm centrifugation 30sec are added in into adsorption column, outwell waste liquid, adsorption column is put back to
In collecting pipe.
(7) step 6 is repeated.
(8) adsorption column is put back in collecting pipe, 12000rpm centrifugation 2min outwell waste liquid.Adsorption column, which is placed in, to be placed at room temperature for
It dries for several minutes.
(9) adsorption column is transferred in a clean centrifuge tube, it is slow vacantly to add in 50 μ L elutions to adsorbed film centre position
Fliud flushing TE, is placed at room temperature for 2-5min, and solution is collected into centrifuge tube, -20 DEG C of preservations by 12000rpm centrifugation 2min.
4th, the detection of DNA mass and concentration
(1) agarose gel electrophoresis detects：Using DL2000 as DNALadder, the genomic DNA and 5 μ L of 2 μ L are taken
Loading buffer mixings are clicked and entered in 1.0% Ago-Gel glue hole, the electrophoresis 30min under the direct current of voltage regulation of 5V/cm
Afterwards, the quality of gel imaging system image checking DNA.
(2) ultraviolet spectrophotometry：With UV spectrophotometer measuring DNA concentration.
5th, primer
1 primer sequence of table
6th, PCR reactions and product purification sequencing
Using above-mentioned primer, with ddH2O is negative control, and positive control is respectively department of plant pathology of China Agricultural University Wu
The positive sporulation (Alternaria solani) that macro professor laboratory provides is learned, to potato plant Alternaria
The total DNA for belonging to pathogen carries out PCR reactions, and reaction system and response procedures are as follows：
2 20 μ L PCR reaction systems of table
The PCR reaction conditions of primer WASF/WASR：95 DEG C of pre-degeneration 1min, 94 DEG C of denaturation 30sec, 54 DEG C are annealed
30sec, 72 DEG C of extension 30sec, 72 DEG C extend 8min eventually, totally 29 cycles.
Band is seen whether after the imaging of PCR product gel imaging system, after purification, be sent to Shanghai life work has PCR product
Limit company is sequenced, after obtaining sequencing result, by blast search nucleic acid database (blastn), be compared with it is homologous
Property analysis.
The result shows that：
Acquisition contains the sample that more than sporulation (Alternaria solani) infects, and extracts blade total DNA, should
With primer WASF/WASR of the present invention, with ddH2O is negative control, and positive control is department of plant pathology of China Agricultural University Wu Xue
The positive alternaria solani sorauer A.solani that macro professor laboratory provides, carries out PCR amplification.Amplify alternaria solani sorauer (A.solani)
270bp (Fig. 1), is determined as positive.Glue is cut to corresponding purpose band afterwards, recycling, is sequenced after purification, sequencing result is shown：
Purpose band sequence and A.solani Histone genes at A.solani 270bp ((Accession JF796059 and
EU617716 BLAST sequence alignments)) are carried out, it is 100% as a result to show homology, is judged as corresponding positive accordingly.
7th, the verification of the specificity of primer
After extracting potato sporulation (Alternaria solani) pure bacterial strain DNA, positive control is Chinese agriculture
The positive alternaria solani sorauer A.solani bacterial strains that department of plant pathology of university Wu Xuehong professors laboratory provides, negative control ddH2O,
It is reacted according to above-mentioned PCR reaction systems and response procedures, 1.5% agarose gel electrophoresis imaging and after taking pictures is compared
The specificity of primer.
8th, the verification of primer sensitivity
It determines corresponding three positives of primer WASF/WASR, positive DNA concentration is diluted to 100ng/ respectively
μ l, 50ng/ μ l, 10ng/ μ l, 1ng/ μ l, 100pg/ μ l, 10pg/ μ l, 100fg/ μ l, obtain 7 groups of dilute samples.It detects respectively
Its nucleotide content determines to carry out the Standard PCR of above-mentioned primer after concentration, by 2.5% agarose gel electrophoresis and take pictures,
Find the greatest dilution of primer pair detection sample.
It is verified by specificity and sensitivity, the results showed that：
With ddH2O is negative control, and positive control is taught laboratory for department of plant pathology of China Agricultural University Wu Xuehong and carried
The positive alternaria solani sorauer Alternaria solani bacterial strains supplied.PCR reaction amplification potatos are carried out using primer WASF/WASR
Plant leaf scab tissue total DNA.Gel electrophoresis imaging results show：The only positive alternaria solani sorauer Alternaria at 270bp
Solani has band (Fig. 2).And the minimum detectable concentration of primer is 10pg (Fig. 3).
9th, Heilongjiang Province each department potato Alternaria belongs to pathogen (predominantly sporulation (Alternaria
Solani distribution)) and composition
2015-2016, the Alternaria of acquisition Heilongjiang Province difference potato main producing region belong to the leaf of tikka class disease
Totally 150 parts of piece incidence tissue, extracts its total DNA respectively, and corresponding leaf spot lesion position isolation and purification culture obtains corresponding
Bacterial strain.Belong to the composition of pathogen with above-mentioned round pcr system identification Heilongjiang Province potato Alternaria, estimate each department
Alternaria belongs to pathogenic distribution ratio.Amplified production uses Universal DNA Purification Kit (TIANGEN
Biochemical technology Co., Ltd) kit carry out after purification, amplified fragments are connect with PUC-T carriers using TA cloning process, turn
Change and enter bacillus coli DH 5 alpha, after the LB plate screenings by containing X-Gal, Amp and IPTG, select the monoclonal bacterium of white
It falls, extraction plasmid carries out PCR verifications, while sends to the sequencing of Shanghai Sheng Gong Co., Ltds.Sequencing result passes through blast search nucleic acid
Database (blastn), is compared and homology analysis, sequence results carry out Multiple sequence alignments point with DNAMAN softwares
Analysis, is finally analyzed with MEGA5.05 softwares.
Sequence table
<110>Plant toxic nursery stock research institute of Heilongjiang Academy of Agricultural Sciences
<120>The molecular detection primer and its detection method of potato plant tikka class disease alternaria solani sorauer pathogen
<160>2
<210> 1
<211>20
<212> DNA
<213>Artificial sequence
<220>
<223>WASF primers.
<400> 1
AAGGACCAACCC ATA AACCT 20
<210>2
<211>18
<212> DNA
<213>Artificial sequence
<220>
<223>WASR primers.
<400> 2
CGCAAGGGGAGACAA AAA 18
Specification
1
10003
2006.7
Claims (5)
1. the molecular detection primer of potato plant tikka class disease alternaria solani sorauer pathogen, it is characterised in that the primer sequence
Row such as sequence table Seq ID No：1 shown and Seq ID No：Shown in 2.
2. the molecular detecting method of potato plant tikka class disease alternaria solani sorauer pathogen, it is characterised in that it is according to following
What step carried out：
First, to extract potato gene group DNA to be detected as template, using the primer of claim 1, PCR amplification is carried out；
2nd, PCR product is taken to carry out detected through gel electrophoresis, if being capable of detecting when the segment of 270bp sizes, can determine whether out to be detected
Potato in there are alternaria solani sorauer pathogens.
3. the molecular detecting method of potato plant tikka class disease alternaria solani sorauer pathogen according to claim 2,
It is sporulation Alternaria solani to be characterized in that the alternaria solani sorauer pathogen.
4. the molecular detecting method of potato plant tikka class disease alternaria solani sorauer pathogen according to claim 2,
It is characterized in that the PCR reaction systems are as follows for 20 μ L：
PCR amplification condition is：5 DEG C of pre-degeneration 1min, 94 DEG C of denaturation 30sec, 54 DEG C of annealing 30sec, 72 DEG C of extension 30sec, 72
DEG C eventually extension 8min, totally 29 cycle.
5. the molecular detecting method of potato plant tikka class disease alternaria solani sorauer pathogen according to claim 2,
It is characterized in that after PCR is detected successfully, by PCR product after purification, is sequenced, carry out Blast comparisons and homology analysis.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3083248A1 (en) * | 2018-06-28 | 2020-01-03 | Anova-Plus | KIT FOR DETECTION OF ALTERNARIOSIS AND DISCRIMINATION BETWEEN ALTERNARIA STRAINS OF THE ALTERNARIA SECTION AND THOSE OF THE PORRI SECTION |
| RU2804687C1 (en) * | 2022-12-30 | 2023-10-03 | Федеральное государственное бюджетное учреждение науки Пермский федеральный исследовательский центр Уральского отделения Российской академии наук (ПФИЦ УрО РАН) | Method for pcr identification of phytopathogenic fungus alternaria solani |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434997A (en) * | 2008-12-23 | 2009-05-20 | 华中农业大学 | Early stage rapid molecular detection method for rape sclerotinia rot |
| CN101501217A (en) * | 2006-05-25 | 2009-08-05 | 孟山都技术有限公司 | A method to identify disease resistant quantitative trait loci in soybean and compositions thereof |
| WO2013140119A2 (en) * | 2012-03-21 | 2013-09-26 | Natural Biotechnology Sprl | Pre-harvest treatment |
| CN103343168A (en) * | 2013-07-26 | 2013-10-09 | 中国农业科学院油料作物研究所 | Rapid identification method for pathogenic fungi |
| CN103484539A (en) * | 2013-08-30 | 2014-01-01 | 福建农林大学 | Group of microsatellite primers for diversity analysis of early blight pathogen alternaria solani |
| CN104120170A (en) * | 2013-04-24 | 2014-10-29 | 河北农业大学 | Potato early blight bacterium detection kit and detection method |
-
2018
- 2018-02-22 CN CN201810154052.2A patent/CN108148923A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101501217A (en) * | 2006-05-25 | 2009-08-05 | 孟山都技术有限公司 | A method to identify disease resistant quantitative trait loci in soybean and compositions thereof |
| CN101434997A (en) * | 2008-12-23 | 2009-05-20 | 华中农业大学 | Early stage rapid molecular detection method for rape sclerotinia rot |
| WO2013140119A2 (en) * | 2012-03-21 | 2013-09-26 | Natural Biotechnology Sprl | Pre-harvest treatment |
| CN104120170A (en) * | 2013-04-24 | 2014-10-29 | 河北农业大学 | Potato early blight bacterium detection kit and detection method |
| CN103343168A (en) * | 2013-07-26 | 2013-10-09 | 中国农业科学院油料作物研究所 | Rapid identification method for pathogenic fungi |
| CN103484539A (en) * | 2013-08-30 | 2014-01-01 | 福建农林大学 | Group of microsatellite primers for diversity analysis of early blight pathogen alternaria solani |
Non-Patent Citations (3)
| Title |
|---|
| GERD STAMMLER等: "Pathogenicity of Alternaria-species on potatoes and tomatoes", 《FOURTEENTH EUROBLIGHT WORKSHOP》 * |
| SUDHEER KUMAR等: "Rapid detection and quantification of Alternaria solani in tomato", 《SCIENTIA HORTICULTURAE》 * |
| 郭倩倩: "马铃薯早疫病的分子检测及田间早期诊断", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3083248A1 (en) * | 2018-06-28 | 2020-01-03 | Anova-Plus | KIT FOR DETECTION OF ALTERNARIOSIS AND DISCRIMINATION BETWEEN ALTERNARIA STRAINS OF THE ALTERNARIA SECTION AND THOSE OF THE PORRI SECTION |
| RU2804687C1 (en) * | 2022-12-30 | 2023-10-03 | Федеральное государственное бюджетное учреждение науки Пермский федеральный исследовательский центр Уральского отделения Российской академии наук (ПФИЦ УрО РАН) | Method for pcr identification of phytopathogenic fungus alternaria solani |
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Application publication date: 20180612 |